pycom13g19370

Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013313
Physical Location & Seq
Reverse (-)
15372162 .. 15373904
1743 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 453 bp
ATGGCAGAGAAGAAAGCAAGCATAACCCACCAGCTTCAGGCCGCGAAGCACAAGTCCGGCAAGTCCTACAGCCAGCTTGCCAAGGAGACGGGCCTCACCAACGTTTATGTTGCCCAGCTTCTCAGGCGCCAAGCTCAGCTCAAGCCTGAGACTGCCCCTAAGTTGCAGGCTGCCCTGCCCGACCTGCCCCAAGAACTCATTCAGGAGATGAAGATTCCACCCTTGAGGTCCTACGATCCTAATTTGATCCAGGACCCCACTATTTACAGGTTGAATGAGGCAGTTATGCATTTTGGTGAAAGCATCAAGGAGATTGTCAATGAGGAATTTGGCGATGGAATTATGTCAGCTATAGACTTCTACTGTTCAGTCGACAAAGTTAAAGGTGTGGATGGCAAGGATCGGGTAGTCCTGACATTTGATGGGAAATATTTACCTCATTCTGAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008824 GO:0016829 GO:0016840
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Cyanate_lyase

Protein Analysis

151

Amino Acids

16.84

Weight (kDa)

6.42

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyanate_lyase PF02560 81 - 148 1.3e-30 Cyanate lyase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23490 AT3G23490 AT3G23490
fragaria_vesca FvH4_4g04220 FvH4_4g04220
malus_domestica MD13G1218700.v1.1
prunus_persica Prupe.1G045500_v2.0.a1
pyrus_communis pycom13g19370
rosa_chinensis RchiOBHm_Chr4g0394701
rosa_laevigata RLG00000009671
rosa_multiflora Rmu_sc0005356.1_g000020 Rmu_sc0005424.1_g000001
rosa_roxburghii Rroxscaffold_5G00339710
rosa_samantha Rh4AG054400 Rh4BG052100 Rh4CG059000 Rh4DG050700
rosa_wichuraiana Rw4G004340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 192
AccB1I GGYRCC 1 cut(s) 126
AccI GTMKAC 1 cut(s) 372
AccII CGCG 1 cut(s) 44
AciI CCGC 1 cut(s) 42
AclI AACGTT 1 cut(s) 102
AclWI GGATC 3 cut(s) 230, 241, 408
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 1 cut(s) 20
AcyI GRCGYC 1 cut(s) 127
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 1 cut(s) 274
AjnI CCWGG 1 cut(s) 249
AjuI GAANNNNNNNTTGG 2 cut(s) 183, 215
AluBI AGCT 6 cut(s) 34, 76, 118, 134, 139, 350
AluI AGCT 6 cut(s) 34, 76, 118, 134, 139, 350
Alw26I GTCTC 2 cut(s) 80, 143
AlwI GGATC 3 cut(s) 230, 241, 408
AoxI GGCC 2 cut(s) 39, 91
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 1 cut(s) 326
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
Asp700I GAANNNNTTC 1 cut(s) 198
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 3 cut(s) 91, 228, 253
AsuHPI GGTGA 2 cut(s) 88, 308
AvaII GGWCC 2 cut(s) 228, 253
BanI GGYRCC 1 cut(s) 126
BbvI GCAGC 1 cut(s) 157
BccI CCATC 3 cut(s) 329, 386, 416
BciT130I CCWGG 1 cut(s) 251
BcoDI GTCTC 2 cut(s) 80, 143
BfmI CTRYAG 2 cut(s) 67, 351
BfoI RGCGCY 1 cut(s) 130
BfuAI ACCTGC 1 cut(s) 192
BisI GCNGC 2 cut(s) 42, 171
BlpI GCTNAGC 1 cut(s) 135
BlsI GCNGC 2 cut(s) 43, 172
Bme1390I CCNGG 1 cut(s) 251
Bme18I GGWCC 2 cut(s) 228, 253
BmgT120I GGNCC 3 cut(s) 91, 228, 253
BmiI GGNNCC 2 cut(s) 128, 255
BmrFI CCNGG 1 cut(s) 251
BmsI GCATC 1 cut(s) 312
Bpu1102I GCTNAGC 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 125, 244
BsaHI GRCGYC 1 cut(s) 127
BsaJI CCNNGG 1 cut(s) 81
Bsc4I CCNNNNNNNGG 1 cut(s) 37
BseBI CCWGG 1 cut(s) 251
BseDI CCNNGG 1 cut(s) 81
BseGI GGATG 1 cut(s) 397
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMII CTCAG 4 cut(s) 136, 138, 149, 435
BseXI GCAGC 1 cut(s) 157
BseYI CCCAGC 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 44
BshFI GGCC 2 cut(s) 41, 93
BshNI GGYRCC 1 cut(s) 126
BsiSI CCGG 1 cut(s) 57
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 2 cut(s) 80, 143
BsmBI CGTCTC 1 cut(s) 80
BsnI GGCC 2 cut(s) 41, 93
Bsp143I GATC 3 cut(s) 235, 246, 400
Bsp1720I GCTNAGC 1 cut(s) 135
BspACI CCGC 1 cut(s) 42
BspANI GGCC 2 cut(s) 41, 93
BspCNI CTCAG 4 cut(s) 135, 139, 148, 436
BspFNI CGCG 1 cut(s) 44
BspLI GGNNCC 2 cut(s) 128, 255
BspMI ACCTGC 1 cut(s) 192
BspPI GGATC 3 cut(s) 230, 241, 408
BspT107I GGYRCC 1 cut(s) 126
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 3 cut(s) 235, 246, 400
BssNI GRCGYC 1 cut(s) 127
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 1 cut(s) 365
BstACI GRCGYC 1 cut(s) 127
BstC8I GCNNGC 4 cut(s) 19, 74, 78, 168
BstDEI CTNAG 5 cut(s) 122, 135, 147, 159, 444
BstF5I GGATG 1 cut(s) 397
BstFNI CGCG 1 cut(s) 44
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 3 cut(s) 238, 249, 403
BstMAI GTCTC 2 cut(s) 80, 143
BstMBI GATC 3 cut(s) 235, 246, 400
BstMWI GCNNNNNNNGC 2 cut(s) 124, 184
BstNI CCWGG 1 cut(s) 251
BstSCI CCNGG 1 cut(s) 249
BstSFI CTRYAG 2 cut(s) 67, 351
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 1 cut(s) 157
BsuRI GGCC 2 cut(s) 41, 93
BtgZI GCGATG 1 cut(s) 348
BtsCI GGATG 1 cut(s) 397
BveI ACCTGC 1 cut(s) 192
Cac8I GCNNGC 4 cut(s) 19, 74, 78, 168
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 3 cut(s) 91, 228, 253
DdeI CTNAG 5 cut(s) 122, 135, 147, 159, 444
DinI GGCGCC 1 cut(s) 128
DpnI GATC 3 cut(s) 237, 248, 402
DpnII GATC 3 cut(s) 235, 246, 400
Eco130I CCWWGG 1 cut(s) 81
Eco47I GGWCC 2 cut(s) 228, 253
Eco57I CTGAAG 1 cut(s) 20
EcoO109I RGGNCCY 2 cut(s) 228, 253
EcoRII CCWGG 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 291
EgeI GGCGCC 1 cut(s) 128
EheI GGCGCC 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 80
FaiI YATR 5 cut(s) 23, 108, 287, 344, 353
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 2 cut(s) 42, 171
FokI GGATG 1 cut(s) 404
Fsp4HI GCNGC 2 cut(s) 42, 171
GlaI GCGC 1 cut(s) 128
GluI GCNGC 2 cut(s) 42, 171
GsaI CCCAGC 1 cut(s) 118
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 2 cut(s) 41, 93
HapII CCGG 1 cut(s) 57
HhaI GCGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 127
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 1 cut(s) 214
HpaII CCGG 1 cut(s) 57
HphI GGTGA 2 cut(s) 88, 308
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 1 cut(s) 445
Hpy188III TCNNGA 2 cut(s) 203, 412
Hpy8I GTNNAC 1 cut(s) 373
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 2 cut(s) 166, 289
HpyF10VI GCNNNNNNNGC 2 cut(s) 124, 184
HpyF3I CTNAG 5 cut(s) 122, 135, 147, 159, 444
HpySE526I ACGT 1 cut(s) 102
Hsp92I GRCGYC 1 cut(s) 127
HspAI GCGC 1 cut(s) 127
KasI GGCGCC 1 cut(s) 126
Kzo9I GATC 3 cut(s) 235, 246, 400
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 1 cut(s) 312
MaeII ACGT 1 cut(s) 102
MalI GATC 3 cut(s) 237, 248, 402
MboI GATC 3 cut(s) 235, 246, 400
MboII GAAGA 2 cut(s) 22, 223
MluCI AATT 3 cut(s) 241, 326, 339
Mly113I GGCGCC 1 cut(s) 127
MnlI CCTC 5 cut(s) 104, 219, 271, 316, 447
Mph1103I ATGCAT 1 cut(s) 291
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 294
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 1 cut(s) 251
MvaI CCWGG 1 cut(s) 251
MvnI CGCG 1 cut(s) 44
MwoI GCNNNNNNNGC 2 cut(s) 124, 184
NarI GGCGCC 1 cut(s) 127
NdeII GATC 3 cut(s) 235, 246, 400
NlaIV GGNNCC 2 cut(s) 128, 255
NsiI ATGCAT 1 cut(s) 291
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 1 cut(s) 214
PfoI TCCNGGA 1 cut(s) 249
PkrI GCNGC 2 cut(s) 43, 172
PluTI GGCGCC 1 cut(s) 130
PpuMI RGGWCCY 2 cut(s) 228, 253
Psp1406I AACGTT 1 cut(s) 102
Psp5II RGGWCCY 2 cut(s) 228, 253
Psp6I CCWGG 1 cut(s) 249
PspFI CCCAGC 1 cut(s) 114
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 2 cut(s) 128, 255
PspPI GGNCC 3 cut(s) 91, 228, 253
PspPPI RGGWCCY 2 cut(s) 228, 253
RseI CAYNNNNRTG 1 cut(s) 294
SalI GTCGAC 1 cut(s) 371
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 2 cut(s) 42, 171
Sau3AI GATC 3 cut(s) 235, 246, 400
Sau96I GGNCC 3 cut(s) 91, 228, 253
ScrFI CCNGG 1 cut(s) 251
SfaNI GCATC 1 cut(s) 312
SfcI CTRYAG 2 cut(s) 67, 351
SfoI GGCGCC 1 cut(s) 128
SinI GGWCC 2 cut(s) 228, 253
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 2 cut(s) 140, 223
SmoI CTYRAG 2 cut(s) 140, 223
Sse9I AATT 3 cut(s) 241, 326, 339
SsiI CCGC 1 cut(s) 42
SspDI GGCGCC 1 cut(s) 126
SspI AATATT 1 cut(s) 431
StyD4I CCNGG 1 cut(s) 249
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 365
TaiI ACGT 1 cut(s) 105
TaqI TCGA 1 cut(s) 372
TasI AATT 3 cut(s) 241, 326, 339
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 1 cut(s) 214
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 224
VpaK11BI GGWCC 2 cut(s) 228, 253
XapI RAATTY 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 372
XmnI GAANNNNTTC 1 cut(s) 198
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.