pycom13g19490

Ubiquitin-binding WIYLD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0020844
Physical Location & Seq
Reverse (-)
15470025 .. 15472454
2430 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
ATGTCCTCTGCAGAACAGGGAGGAGGAGGAGAAATAAGACCTTCCCAAAAGCCCATTTCTAAGAGAGGCCACGACAATGTTCAAGAGGCCCACGTGTTACCCGGCAGACAACCCTACGCCGCTCTCTCTCTATATATACATCTCCTAAACCCTAATCGCTCTCTCAGTCCCGTTTCCGCAGCAGCCAAGATGTCGAAGCGAGGGCGTGGAGGGTCCGCTGGAAACAAGTTTAGGATGTCACTGGGTCTTCCAGTGGCAGCCACTGTGAACTGTGCTGATAACACCGGAGCTAAGAATCTTTACATCATTTCCGTGAAGGGGATCAAGGGTCGTTTGAACAGGTTGCCATCTGCTTGCGTTGGTGACATGGTCATGGCCACTGTCAAGAAGGGAAAGCCTGACCTCAGGAAGAAGGTCCTGCCTGCTGTCATCGTGAGGCAGCGCAAGCCATGGCGCCGAAAGGATGGTGTTTTCATGTACTTTGAAGATAATGCTGGTGTTATTGTGAATCCGAAGGGAGAAATGAAAGGGTCTGCTATTACTGGTCCGATCGGAAAGGAGTGTGCTGATCTCTGGCCTAGGATTGCAAGTGCTGCTAATGCCATCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Pre-SET Ribosomal_L14 SET WIYLD

Protein Analysis

204

Amino Acids

21.67

Weight (kDa)

10.4

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L14 PF00238 84 - 203 3.8e-41 Ribosomal protein L14p/L23e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020844)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04480 AT2G33370 AT3G04400
pyrus_communis pycom13g19490
rosa_wichuraiana Rw7G016970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 453
AccBSI CCGCTC 1 cut(s) 122
AciI CCGC 3 cut(s) 120, 177, 216
AclWI GGATC 1 cut(s) 329
AcoI YGGCCR 1 cut(s) 375
AcvI CACGTG 1 cut(s) 94
AcyI GRCGYC 1 cut(s) 454
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 1 cut(s) 318
AflIII ACRYGT 1 cut(s) 93
AgsI TTSAA 3 cut(s) 83, 337, 485
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
AlwI GGATC 1 cut(s) 329
AlwNI CAGNNNCTG 1 cut(s) 263
AoxI GGCC 4 cut(s) 67, 87, 375, 575
ApeKI GCWGC 5 cut(s) 179, 182, 257, 439, 593
AspA2I CCTAGG 1 cut(s) 578
AspLEI GCGC 2 cut(s) 444, 456
AspS9I GGNCC 4 cut(s) 88, 213, 415, 545
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 374
AvaII GGWCC 3 cut(s) 213, 415, 545
AvrII CCTAGG 1 cut(s) 578
AxyI CCTNAGG 1 cut(s) 404
BalI TGGCCA 1 cut(s) 377
BanI GGYRCC 1 cut(s) 453
BbrPI CACGTG 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 239
BbvI GCAGC 5 cut(s) 191, 194, 269, 451, 580
BccI CCATC 3 cut(s) 355, 458, 611
BcnI CCSGG 1 cut(s) 102
BfaI CTAG 1 cut(s) 579
BfmI CTRYAG 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 457
BisI GCNGC 6 cut(s) 120, 180, 183, 258, 440, 594
BlnI CCTAGG 1 cut(s) 578
BlsI GCNGC 6 cut(s) 121, 181, 184, 259, 441, 595
Bme1390I CCNGG 1 cut(s) 102
Bme18I GGWCC 3 cut(s) 213, 415, 545
BmgT120I GGNCC 4 cut(s) 88, 213, 415, 545
BmiI GGNNCC 2 cut(s) 214, 455
BmrFI CCNGG 1 cut(s) 102
BmrI ACTGGG 1 cut(s) 251
BmuI ACTGGG 1 cut(s) 251
BpiI GAAGAC 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 102
BsaAI YACGTR 1 cut(s) 94
BsaHI GRCGYC 1 cut(s) 454
BsaJI CCNNGG 2 cut(s) 449, 578
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse1I ACTGG 3 cut(s) 246, 251, 547
Bse21I CCTNAGG 1 cut(s) 404
BseDI CCNNGG 2 cut(s) 449, 578
BseGI GGATG 2 cut(s) 240, 469
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMII CTCAG 2 cut(s) 178, 418
BseNI ACTGG 3 cut(s) 246, 251, 547
BseRI GAGGAG 3 cut(s) 36, 39, 42
BseXI GCAGC 5 cut(s) 191, 194, 269, 451, 580
Bsh1285I CGRYCG 1 cut(s) 552
BshFI GGCC 4 cut(s) 69, 89, 377, 577
BshNI GGYRCC 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 552
BsiSI CCGG 2 cut(s) 102, 285
BslFI GGGAC 1 cut(s) 153
BslI CCNNNNNNNGG 1 cut(s) 318
BsmFI GGGAC 1 cut(s) 153
BsnI GGCC 4 cut(s) 69, 89, 377, 577
Bsp143I GATC 3 cut(s) 321, 549, 568
Bsp19I CCATGG 1 cut(s) 449
BspACI CCGC 3 cut(s) 120, 177, 216
BspANI GGCC 4 cut(s) 69, 89, 377, 577
BspCNI CTCAG 2 cut(s) 177, 417
BspLI GGNNCC 2 cut(s) 214, 455
BspMAI CTGCAG 1 cut(s) 13
BspPI GGATC 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 453
BsrBI CCGCTC 1 cut(s) 122
BsrI ACTGG 3 cut(s) 246, 251, 547
BssECI CCNNGG 2 cut(s) 449, 578
BssMI GATC 3 cut(s) 321, 549, 568
BssNI GRCGYC 1 cut(s) 454
BssT1I CCWWGG 2 cut(s) 449, 578
Bst4CI ACNGT 3 cut(s) 265, 272, 382
BstACI GRCGYC 1 cut(s) 454
BstAPI GCANNNNNTGC 1 cut(s) 593
BstBAI YACGTR 1 cut(s) 94
BstC8I GCNNGC 3 cut(s) 355, 423, 446
BstDEI CTNAG 4 cut(s) 60, 164, 291, 404
BstDSI CCRYGG 1 cut(s) 449
BstF5I GGATG 2 cut(s) 240, 469
BstH2I RGCGCY 1 cut(s) 457
BstHHI GCGC 2 cut(s) 444, 456
BstKTI GATC 3 cut(s) 324, 552, 571
BstMBI GATC 3 cut(s) 321, 549, 568
BstMCI CGRYCG 1 cut(s) 552
BstMWI GCNNNNNNNGC 3 cut(s) 445, 593, 599
BstSCI CCNGG 1 cut(s) 100
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 5 cut(s) 191, 194, 269, 451, 580
BstV2I GAAGAC 1 cut(s) 239
Bsu36I CCTNAGG 1 cut(s) 404
BsuRI GGCC 4 cut(s) 69, 89, 377, 577
BtgI CCRYGG 1 cut(s) 449
BtsCI GGATG 2 cut(s) 240, 469
BtsIMutI CAGTG 4 cut(s) 239, 258, 261, 378
Cac8I GCNNGC 3 cut(s) 355, 423, 446
CaiI CAGNNNCTG 1 cut(s) 263
CfoI GCGC 2 cut(s) 444, 456
Cfr13I GGNCC 4 cut(s) 88, 213, 415, 545
Csp6I GTAC 1 cut(s) 478
CviAII CATG 4 cut(s) 367, 373, 450, 475
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 4 cut(s) 60, 164, 291, 404
DinI GGCGCC 1 cut(s) 455
DpnI GATC 3 cut(s) 323, 551, 570
DpnII GATC 3 cut(s) 321, 549, 568
EaeI YGGCCR 1 cut(s) 375
Eco130I CCWWGG 2 cut(s) 449, 578
Eco47I GGWCC 3 cut(s) 213, 415, 545
Eco72I CACGTG 1 cut(s) 94
Eco81I CCTNAGG 1 cut(s) 404
EcoO109I RGGNCCY 1 cut(s) 415
EcoT14I CCWWGG 2 cut(s) 449, 578
EgeI GGCGCC 1 cut(s) 455
EheI GGCGCC 1 cut(s) 455
ErhI CCWWGG 2 cut(s) 449, 578
FaeI CATG 4 cut(s) 370, 376, 453, 478
FaiI YATR 7 cut(s) 133, 135, 137, 368, 374, 451, 476
FaqI GGGAC 1 cut(s) 153
FatI CATG 4 cut(s) 366, 372, 449, 474
Fnu4HI GCNGC 6 cut(s) 120, 180, 183, 258, 440, 594
FokI GGATG 2 cut(s) 247, 476
Fsp4HI GCNGC 6 cut(s) 120, 180, 183, 258, 440, 594
FspBI CTAG 1 cut(s) 579
GlaI GCGC 2 cut(s) 443, 455
GluI GCNGC 6 cut(s) 120, 180, 183, 258, 440, 594
HaeII RGCGCY 1 cut(s) 457
HaeIII GGCC 4 cut(s) 69, 89, 377, 577
HapII CCGG 2 cut(s) 102, 285
HhaI GCGC 2 cut(s) 444, 456
Hin1I GRCGYC 1 cut(s) 454
Hin1II CATG 4 cut(s) 370, 376, 453, 478
Hin6I GCGC 2 cut(s) 442, 454
HinP1I GCGC 2 cut(s) 442, 454
HinfI GANTC 2 cut(s) 295, 508
HpaII CCGG 2 cut(s) 102, 285
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 4 cut(s) 513, 549, 554, 611
Hpy188III TCNNGA 4 cut(s) 83, 385, 406, 433
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 5 cut(s) 51, 310, 382, 406, 508
HpyCH4III ACNGT 3 cut(s) 265, 272, 382
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 2 cut(s) 11, 587
HpyF10VI GCNNNNNNNGC 3 cut(s) 445, 593, 599
HpyF3I CTNAG 4 cut(s) 60, 164, 291, 404
HpySE526I ACGT 1 cut(s) 93
Hsp92I GRCGYC 1 cut(s) 454
Hsp92II CATG 4 cut(s) 370, 376, 453, 478
HspAI GCGC 2 cut(s) 442, 454
KasI GGCGCC 1 cut(s) 453
Kzo9I GATC 3 cut(s) 321, 549, 568
LmnI GCTCC 1 cut(s) 287
Lsp1109I GCAGC 5 cut(s) 191, 194, 269, 451, 580
MaeI CTAG 1 cut(s) 579
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 3 cut(s) 96, 237, 362
MalI GATC 3 cut(s) 323, 551, 570
MbiI CCGCTC 1 cut(s) 122
MboI GATC 3 cut(s) 321, 549, 568
MboII GAAGA 3 cut(s) 239, 421, 497
MlsI TGGCCA 1 cut(s) 377
MluNI TGGCCA 1 cut(s) 377
Mly113I GGCGCC 1 cut(s) 454
Mox20I TGGCCA 1 cut(s) 377
MscI TGGCCA 1 cut(s) 377
MslI CAYNNNNRTG 3 cut(s) 75, 311, 371
Msp20I TGGCCA 1 cut(s) 377
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 2 cut(s) 102, 285
MspR9I CCNGG 1 cut(s) 102
MwoI GCNNNNNNNGC 3 cut(s) 445, 593, 599
NarI GGCGCC 1 cut(s) 454
NciI CCSGG 1 cut(s) 102
NcoI CCATGG 1 cut(s) 449
NdeII GATC 3 cut(s) 321, 549, 568
NlaIII CATG 4 cut(s) 370, 376, 453, 478
NlaIV GGNNCC 2 cut(s) 214, 455
NmuCI GTSAC 2 cut(s) 237, 362
PfeI GAWTC 2 cut(s) 295, 508
PflFI GACNNNGTC 1 cut(s) 368
PkrI GCNGC 6 cut(s) 121, 181, 184, 259, 441, 595
Ple19I CGATCG 1 cut(s) 552
PluTI GGCGCC 1 cut(s) 457
PmaCI CACGTG 1 cut(s) 94
PmlI CACGTG 1 cut(s) 94
Ppu21I YACGTR 1 cut(s) 94
PpuMI RGGWCCY 1 cut(s) 415
Psp5II RGGWCCY 1 cut(s) 415
PspCI CACGTG 1 cut(s) 94
PspN4I GGNNCC 2 cut(s) 214, 455
PspPI GGNCC 4 cut(s) 88, 213, 415, 545
PspPPI RGGWCCY 1 cut(s) 415
PstI CTGCAG 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 368
PvuI CGATCG 1 cut(s) 552
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
RseI CAYNNNNRTG 3 cut(s) 75, 311, 371
SatI GCNGC 6 cut(s) 120, 180, 183, 258, 440, 594
Sau3AI GATC 3 cut(s) 321, 549, 568
Sau96I GGNCC 4 cut(s) 88, 213, 415, 545
ScrFI CCNGG 1 cut(s) 102
SetI ASST 6 cut(s) 43, 96, 292, 344, 405, 417
SfcI CTRYAG 1 cut(s) 9
SfoI GGCGCC 1 cut(s) 455
SinI GGWCC 3 cut(s) 213, 415, 545
SmiMI CAYNNNNRTG 3 cut(s) 75, 311, 371
SsiI CCGC 3 cut(s) 120, 177, 216
SspDI GGCGCC 1 cut(s) 453
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 100
StyI CCWWGG 2 cut(s) 449, 578
TaaI ACNGT 3 cut(s) 265, 272, 382
TaiI ACGT 1 cut(s) 96
TaqI TCGA 1 cut(s) 194
TatI WGTACW 1 cut(s) 477
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 2 cut(s) 295, 508
TscAI CASTG 4 cut(s) 246, 258, 268, 385
TseFI GTSAC 2 cut(s) 237, 362
TseI GCWGC 5 cut(s) 179, 182, 257, 439, 593
Tsp45I GTSAC 2 cut(s) 237, 362
TspDTI ATGAA 2 cut(s) 463, 539
TspGWI ACGGA 1 cut(s) 301
TspRI CASTG 4 cut(s) 246, 258, 268, 385
Tth111I GACNNNGTC 1 cut(s) 368
VpaK11BI GGWCC 3 cut(s) 213, 415, 545
XmaJI CCTAGG 1 cut(s) 578
XspI CTAG 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.