pycom13g26120

Retroviral aspartyl protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0020125
Physical Location & Seq
Forward (+)
22854774 .. 22856883
2110 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g26120.4

Sequence Viewer

Length: 1761 bp
ATGATGGCCTACTTTGACCAGTTCTGTCATGAACTCTCCTATGGGGGTGGGTGTGCATCCTCCTCTGAAACTGCTCTGATCTGGAACAGTAGCGCCAACTCCGTCGCGAGATGTGTGAGTTCGGTGCTCTGTTTCCACGTCCTCAATGGCAACCGAGGATTGACTTTGATTGTTACATGGACGGAGATGATCCGCTCGCCTGGACTTACAAAGCGGAGTAGTTTTTTCACTACTACAACACACTGGATGCCCAGAAAGTTATTACAACCTCCTTTCATCTTGAAGGCAAGGGACTGCAATGGTTCCGCTGGTTTGACTGTATCAATTCCACTCCCCACTGGCCGGTGTTCACTAAGGCTTTCTGCAGGGAGTTTGGCCCTTTTGAATTCGAGGATAATGTTAGTCAATCGAACTTCTGATTTAGGTCATGTTTTACTCAAGAGTTGTTTCCTGGGGGGATTGAGGTGTAAATTAAAGTTTGATGTCAGATTATTTAAGCCCGTTACTGTTCATGAAGCAATTGCACTTGCTGTTCAATTAGATGAAAAATTGTCTGAGCTCAAGTCTTCCACCGCTAAGTTTGCCCAACCTATTAAACTTGCACCTACACCACCAGCCTTACCACATGCCATTCCCAGAAATAGGCTAGTAGGACTACTTTTCAAGAAGTTAACTCATGTTGAAGTACAGCGGGGTGGCTCAGTGAACAATCAACCAGTTAGGATTTTACTAGATTCTGGCAGCACACATAATTTTGTGGACTCTAGACTAGTGAAACACTTAGGTTGGCAAAGTAAGAAGTCAACGTTGCTTCGGACAGGTAGCCTTGGAAATTGGGGGGTATACGTGCTTTTCCACCCCTTATTCTCTACCCTTAAGAGGGTGTGGTGTGGTTCTTGGAGTATAATGGTTTTCTATAGTGAGTCTTGTGTTATGGGACTTTCAATTGCTCACAATGGAATTCTCTGTGGGGCAAAACCAATTCAAATTCAGCTTGACAAGGAATTTTGTAATTCGAACTTGGGTATGTTTCTTTATTCTATGGATGCCAACAAGAGGGAAGAACTCACACTAGACGGTACACAACTCAATGAGTTGGAAGCATTGTTGGAGGTGGTCTCTAGAGCCAAGCCACCAAATATTAGGCCATACCACTATGGTCCCTTGCAGAAAACTGAGATAGAGAAAGAAATGCAAGAATTACTCCAAGCTGGTTTTATTTGTCCGAGCCACATCCCATTCTCATTTCCAATGCTATTGGTTAGAAAGAAAGAGGGTACTTGGAGACTTTGCATGGATTATAGGGAACTCAAGTCCTTGAAAATCAAATACAAATATCCCATACCCTTGATCGATGACTTGTTGGATGAGTTATTTGGAGCCAAGTACTTTTCAAAGCTTGACTTAAGATCGGGCTACCATCAAATTCGAATGAATTCTAGAGATGTGGAAAAGACAACCTTTAGGACTCATGAGGGGCACTATAAGTTCTTAGTTATGCCATTTAGGCTAACTAATGCCCCTGCTTCTTTTCAAAGTTTGATGAATGATGTTTTCAAAGCTCACCTGAGAAAATTCATGCTAGTCCTCTTTGATGATATCCTAATTTACAGCAAGTCTTGGAACAAGCATTTACAACATTTGCACACTACTTTTGAGGATCCTGCAACCATCAGGCAATCGAATCCTATAAGCTACTGGACCAATTATTTCTTTCACTTCAAATGGTCCATAGTACTTAGGATACAGCTTGTGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag

Protein Analysis

587

Amino Acids

66.7

Weight (kDa)

9.58

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020125)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19792 FvH4_4g07791
pyrus_communis pycom13g26120 pycom16g15860
rosa_laevigata RLG00000022349
rosa_roxburghii Rroxscaffold_3G00238350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 195
AccI GTMKAC 1 cut(s) 843
AccII CGCG 1 cut(s) 107
AciI CCGC 5 cut(s) 193, 214, 306, 573, 691
AclI AACGTT 1 cut(s) 806
AclWI GGATC 3 cut(s) 184, 1655, 1668
AcoI YGGCCR 1 cut(s) 340
AcsI RAATTY 7 cut(s) 385, 960, 987, 1004, 1425, 1435, 1574
AfaI GTAC 5 cut(s) 687, 1081, 1279, 1388, 1737
AfiI CCNNNNNNNGG 5 cut(s) 342, 642, 879, 880, 1056
AflII CTTAAG 2 cut(s) 875, 1405
AhlI ACTAGT 1 cut(s) 769
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 2 cut(s) 199, 450
AluBI AGCT 7 cut(s) 559, 994, 1211, 1399, 1562, 1695, 1750
AluI AGCT 7 cut(s) 559, 994, 1211, 1399, 1562, 1695, 1750
Alw21I GWGCWC 2 cut(s) 129, 561
Alw26I GTCTC 2 cut(s) 1123, 1279
AlwI GGATC 3 cut(s) 184, 1655, 1668
AoxI GGCC 4 cut(s) 6, 340, 375, 1145
ApeKI GCWGC 1 cut(s) 741
ApoI RAATTY 7 cut(s) 385, 960, 987, 1004, 1425, 1435, 1574
Asp700I GAANNNNTTC 1 cut(s) 1435
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 4 cut(s) 376, 1160, 1701, 1728
AsuHPI GGTGA 1 cut(s) 1556
AsuII TTCGAA 2 cut(s) 1016, 1429
AvaII GGWCC 3 cut(s) 1160, 1701, 1728
BaeGI GKGCMC 1 cut(s) 1482
BamHI GGATCC 1 cut(s) 1660
BanII GRGCYC 1 cut(s) 561
BbsI GAAGAC 1 cut(s) 558
Bbv12I GWGCWC 2 cut(s) 129, 561
BbvI GCAGC 1 cut(s) 753
BccI CCATC 2 cut(s) 1428, 1679
BciT130I CCWGG 2 cut(s) 201, 452
BciVI GTATCC 1 cut(s) 1737
BcoDI GTCTC 2 cut(s) 1123, 1279
BcuI ACTAGT 1 cut(s) 769
BfaI CTAG 8 cut(s) 647, 731, 765, 770, 1073, 1122, 1440, 1583
BfmI CTRYAG 2 cut(s) 363, 916
BfoI RGCGCY 1 cut(s) 96
BfrI CTTAAG 2 cut(s) 875, 1405
BfuI GTATCC 1 cut(s) 1737
BglI GCCNNNNNGGC 1 cut(s) 1507
BisI GCNGC 1 cut(s) 742
BlsI GCNGC 1 cut(s) 743
BmcAI AGTACT 2 cut(s) 1388, 1737
Bme1390I CCNGG 2 cut(s) 201, 452
Bme18I GGWCC 3 cut(s) 1160, 1701, 1728
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 4 cut(s) 376, 1160, 1701, 1728
BmiI GGNNCC 4 cut(s) 304, 1162, 1381, 1662
BmrFI CCNGG 2 cut(s) 201, 452
BmsI GCATC 3 cut(s) 65, 237, 1036
BpiI GAAGAC 1 cut(s) 558
BplI GAGNNNNNCTC 2 cut(s) 1103, 1135
Bpu14I TTCGAA 2 cut(s) 1016, 1429
BpuEI CTTGAG 3 cut(s) 422, 545, 1295
Bsa29I ATCGAT 1 cut(s) 1353
BsaAI YACGTR 1 cut(s) 847
BsaI GGTCTC 1 cut(s) 1123
BsaJI CCNNGG 3 cut(s) 154, 451, 826
Bsc4I CCNNNNNNNGG 5 cut(s) 342, 642, 879, 880, 1056
Bse118I RCCGGY 1 cut(s) 342
Bse1I ACTGG 5 cut(s) 19, 248, 343, 716, 1703
Bse3DI GCAATG 1 cut(s) 304
BseBI CCWGG 2 cut(s) 201, 452
BseCI ATCGAT 1 cut(s) 1353
BseDI CCNNGG 3 cut(s) 154, 451, 826
BseGI GGATG 5 cut(s) 56, 252, 1051, 1233, 1372
BseLI CCNNNNNNNGG 5 cut(s) 342, 642, 879, 880, 1056
BseMI GCAATG 1 cut(s) 304
BseMII CTCAG 4 cut(s) 546, 714, 1167, 1559
BseNI ACTGG 5 cut(s) 19, 248, 343, 716, 1703
BseRI GAGGAG 1 cut(s) 52
BseSI GKGCMC 1 cut(s) 1482
BseXI GCAGC 1 cut(s) 753
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 4 cut(s) 8, 342, 377, 1147
BshVI ATCGAT 1 cut(s) 1353
BsiHKAI GWGCWC 2 cut(s) 129, 561
BsiSI CCGG 1 cut(s) 343
BslFI GGGAC 3 cut(s) 305, 951, 1146
BslI CCNNNNNNNGG 5 cut(s) 342, 642, 879, 880, 1056
BsmAI GTCTC 2 cut(s) 1123, 1279
BsmFI GGGAC 3 cut(s) 305, 951, 1146
BsnI GGCC 4 cut(s) 8, 342, 377, 1147
Bso31I GGTCTC 1 cut(s) 1123
Bsp119I TTCGAA 2 cut(s) 1016, 1429
Bsp1286I GDGCHC 3 cut(s) 129, 561, 1482
Bsp143I GATC 5 cut(s) 78, 189, 1350, 1409, 1660
Bsp68I TCGCGA 1 cut(s) 107
BspACI CCGC 5 cut(s) 193, 214, 306, 573, 691
BspANI GGCC 4 cut(s) 8, 342, 377, 1147
BspCNI CTCAG 4 cut(s) 547, 713, 1168, 1560
BspDI ATCGAT 1 cut(s) 1353
BspFNI CGCG 1 cut(s) 107
BspHI TCATGA 3 cut(s) 28, 511, 1471
BspLI GGNNCC 4 cut(s) 304, 1162, 1381, 1662
BspMAI CTGCAG 1 cut(s) 367
BspPI GGATC 3 cut(s) 184, 1655, 1668
BspT104I TTCGAA 2 cut(s) 1016, 1429
BspTI CTTAAG 2 cut(s) 875, 1405
BspTNI GGTCTC 1 cut(s) 1123
BsrBI CCGCTC 1 cut(s) 195
BsrDI GCAATG 1 cut(s) 304
BsrFI RCCGGY 1 cut(s) 342
BsrI ACTGG 5 cut(s) 19, 248, 343, 716, 1703
BssAI RCCGGY 1 cut(s) 342
BssECI CCNNGG 3 cut(s) 154, 451, 826
BssMI GATC 5 cut(s) 78, 189, 1350, 1409, 1660
BssNAI GTATAC 1 cut(s) 844
BssT1I CCWWGG 1 cut(s) 826
Bst1107I GTATAC 1 cut(s) 844
Bst2UI CCWGG 2 cut(s) 201, 452
Bst4CI ACNGT 4 cut(s) 89, 319, 508, 1079
BstAFI CTTAAG 2 cut(s) 875, 1405
BstBAI YACGTR 1 cut(s) 847
BstBI TTCGAA 2 cut(s) 1016, 1429
BstC8I GCNNGC 1 cut(s) 197
BstDEI CTNAG 9 cut(s) 353, 555, 576, 700, 781, 1176, 1492, 1568, 1739
BstF5I GGATG 5 cut(s) 56, 252, 1051, 1233, 1372
BstFNI CGCG 1 cut(s) 107
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 5 cut(s) 81, 192, 1353, 1412, 1663
BstMAI GTCTC 2 cut(s) 1123, 1279
BstMBI GATC 5 cut(s) 78, 189, 1350, 1409, 1660
BstMWI GCNNNNNNNGC 2 cut(s) 581, 1507
BstNI CCWGG 2 cut(s) 201, 452
BstNSI RCATGY 1 cut(s) 629
BstSCI CCNGG 2 cut(s) 199, 450
BstSFI CTRYAG 2 cut(s) 363, 916
BstSLI GKGCMC 1 cut(s) 1482
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 1 cut(s) 753
BstV2I GAAGAC 1 cut(s) 558
BstX2I RGATCY 1 cut(s) 1660
BstYI RGATCY 1 cut(s) 1660
BstZ17I GTATAC 1 cut(s) 844
Bsu15I ATCGAT 1 cut(s) 1353
BsuI GTATCC 1 cut(s) 1737
BsuRI GGCC 4 cut(s) 8, 342, 377, 1147
BsuTUI ATCGAT 1 cut(s) 1353
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 5 cut(s) 56, 252, 1051, 1233, 1372
BtsIMutI CAGTG 3 cut(s) 241, 336, 708
BtuMI TCGCGA 1 cut(s) 107
Cac8I GCNNGC 1 cut(s) 197
CciI TCATGA 3 cut(s) 28, 511, 1471
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 1 cut(s) 342
Cfr13I GGNCC 4 cut(s) 376, 1160, 1701, 1728
ClaI ATCGAT 1 cut(s) 1353
Csp6I GTAC 5 cut(s) 686, 1080, 1278, 1387, 1736
CviAII CATG 9 cut(s) 29, 177, 428, 512, 626, 677, 1294, 1472, 1579
CviQI GTAC 5 cut(s) 686, 1080, 1278, 1387, 1736
DdeI CTNAG 9 cut(s) 353, 555, 576, 700, 781, 1176, 1492, 1568, 1739
DpnI GATC 5 cut(s) 80, 191, 1352, 1411, 1662
DpnII GATC 5 cut(s) 78, 189, 1350, 1409, 1660
EaeI YGGCCR 1 cut(s) 340
Ecl136II GAGCTC 1 cut(s) 559
Eco130I CCWWGG 1 cut(s) 826
Eco24I GRGCYC 1 cut(s) 561
Eco31I GGTCTC 1 cut(s) 1123
Eco32I GATATC 1 cut(s) 1600
Eco47I GGWCC 3 cut(s) 1160, 1701, 1728
Eco53kI GAGCTC 1 cut(s) 559
EcoICRI GAGCTC 1 cut(s) 559
EcoRI GAATTC 3 cut(s) 385, 960, 1435
EcoRII CCWGG 2 cut(s) 199, 450
EcoRV GATATC 1 cut(s) 1600
EcoT14I CCWWGG 1 cut(s) 826
EcoT38I GRGCYC 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 826
FaeI CATG 9 cut(s) 32, 180, 431, 515, 629, 680, 1297, 1475, 1582
FalI AAGNNNNNCTT 4 cut(s) 1388, 1420, 1445, 1477
FaqI GGGAC 3 cut(s) 305, 951, 1146
FatI CATG 9 cut(s) 28, 176, 427, 511, 625, 676, 1293, 1471, 1578
FauI CCCGC 1 cut(s) 684
FblI GTMKAC 1 cut(s) 843
Fnu4HI GCNGC 1 cut(s) 742
FokI GGATG 5 cut(s) 43, 259, 1058, 1220, 1379
FriOI GRGCYC 1 cut(s) 561
Fsp4HI GCNGC 1 cut(s) 742
FspBI CTAG 8 cut(s) 647, 731, 765, 770, 1073, 1122, 1440, 1583
GlaI GCGC 1 cut(s) 94
GluI GCNGC 1 cut(s) 742
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 4 cut(s) 8, 342, 377, 1147
HapII CCGG 1 cut(s) 343
HhaI GCGC 1 cut(s) 95
Hin1II CATG 9 cut(s) 32, 180, 431, 515, 629, 680, 1297, 1475, 1582
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HincII GTYRAC 2 cut(s) 672, 804
HindII GTYRAC 2 cut(s) 672, 804
HindIII AAGCTT 1 cut(s) 1397
HinfI GANTC 5 cut(s) 734, 761, 923, 1468, 1684
HpaI GTTAAC 1 cut(s) 672
HpaII CCGG 1 cut(s) 343
HphI GGTGA 1 cut(s) 1556
Hpy166II GTNNAC 7 cut(s) 350, 672, 706, 760, 804, 844, 1082
Hpy188I TCNGA 7 cut(s) 67, 78, 418, 488, 556, 816, 1227
Hpy8I GTNNAC 7 cut(s) 350, 672, 706, 760, 804, 844, 1082
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 1 cut(s) 277
HpyCH4III ACNGT 4 cut(s) 89, 319, 508, 1079
HpyCH4IV ACGT 3 cut(s) 138, 806, 846
HpyF10VI GCNNNNNNNGC 2 cut(s) 581, 1507
HpyF3I CTNAG 9 cut(s) 353, 555, 576, 700, 781, 1176, 1492, 1568, 1739
HpySE526I ACGT 3 cut(s) 138, 806, 846
Hsp92II CATG 9 cut(s) 32, 180, 431, 515, 629, 680, 1297, 1475, 1582
HspAI GCGC 1 cut(s) 93
KspAI GTTAAC 1 cut(s) 672
Kzo9I GATC 5 cut(s) 78, 189, 1350, 1409, 1660
LmnI GCTCC 1 cut(s) 1379
Lsp1109I GCAGC 1 cut(s) 753
LweI GCATC 3 cut(s) 65, 237, 1036
MaeI CTAG 8 cut(s) 647, 731, 765, 770, 1073, 1122, 1440, 1583
MaeII ACGT 3 cut(s) 138, 806, 846
MaeIII GTNAC 2 cut(s) 172, 502
MalI GATC 5 cut(s) 80, 191, 1352, 1411, 1662
MbiI CCGCTC 1 cut(s) 195
MboI GATC 5 cut(s) 78, 189, 1350, 1409, 1660
MboII GAAGA 2 cut(s) 558, 1073
MfeI CAATTG 2 cut(s) 519, 945
MflI RGATCY 1 cut(s) 1660
MhlI GDGCHC 3 cut(s) 129, 561, 1482
MlyI GAGTC 3 cut(s) 755, 932, 1462
MmeI TCCRAC 3 cut(s) 1077, 1089, 1344
MroXI GAANNNNTTC 1 cut(s) 1435
MseI TTAA 6 cut(s) 473, 495, 594, 671, 876, 1406
MspA1I CMGCKG 2 cut(s) 308, 691
MspCI CTTAAG 2 cut(s) 875, 1405
MspI CCGG 1 cut(s) 343
MspR9I CCNGG 2 cut(s) 201, 452
MunI CAATTG 2 cut(s) 519, 945
MvaI CCWGG 2 cut(s) 201, 452
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 2 cut(s) 581, 1507
NdeII GATC 5 cut(s) 78, 189, 1350, 1409, 1660
NlaIII CATG 9 cut(s) 32, 180, 431, 515, 629, 680, 1297, 1475, 1582
NlaIV GGNNCC 4 cut(s) 304, 1162, 1381, 1662
NruI TCGCGA 1 cut(s) 107
NspI RCATGY 1 cut(s) 629
NspV TTCGAA 2 cut(s) 1016, 1429
PagI TCATGA 3 cut(s) 28, 511, 1471
PdmI GAANNNNTTC 1 cut(s) 1435
PfeI GAWTC 2 cut(s) 734, 1684
PkrI GCNGC 1 cut(s) 743
PleI GAGTC 3 cut(s) 755, 931, 1462
PpsI GAGTC 3 cut(s) 755, 931, 1462
Ppu21I YACGTR 1 cut(s) 847
Psp124BI GAGCTC 1 cut(s) 561
Psp1406I AACGTT 1 cut(s) 806
Psp6I CCWGG 2 cut(s) 199, 450
PspGI CCWGG 2 cut(s) 199, 450
PspN4I GGNNCC 4 cut(s) 304, 1162, 1381, 1662
PspPI GGNCC 4 cut(s) 376, 1160, 1701, 1728
PstI CTGCAG 1 cut(s) 367
PsuI RGATCY 1 cut(s) 1660
RruI TCGCGA 1 cut(s) 107
RsaI GTAC 5 cut(s) 687, 1081, 1279, 1388, 1737
RsaNI GTAC 5 cut(s) 686, 1080, 1278, 1387, 1736
SacI GAGCTC 1 cut(s) 561
SaqAI TTAA 6 cut(s) 473, 495, 594, 671, 876, 1406
SatI GCNGC 1 cut(s) 742
Sau3AI GATC 5 cut(s) 78, 189, 1350, 1409, 1660
Sau96I GGNCC 4 cut(s) 376, 1160, 1701, 1728
ScaI AGTACT 2 cut(s) 1388, 1737
SchI GAGTC 3 cut(s) 755, 932, 1462
ScrFI CCNGG 2 cut(s) 201, 452
SduI GDGCHC 3 cut(s) 129, 561, 1482
SfaNI GCATC 3 cut(s) 65, 237, 1036
SfcI CTRYAG 2 cut(s) 363, 916
SfuI TTCGAA 2 cut(s) 1016, 1429
SinI GGWCC 3 cut(s) 1160, 1701, 1728
SmlI CTYRAG 5 cut(s) 437, 560, 875, 1310, 1405
SmoI CTYRAG 5 cut(s) 437, 560, 875, 1310, 1405
SpeI ACTAGT 1 cut(s) 769
SsiI CCGC 5 cut(s) 193, 214, 306, 573, 691
SspI AATATT 1 cut(s) 1141
SspMI CTAG 8 cut(s) 647, 731, 765, 770, 1073, 1122, 1440, 1583
SstI GAGCTC 1 cut(s) 561
StyD4I CCNGG 2 cut(s) 199, 450
StyI CCWWGG 1 cut(s) 826
TaaI ACNGT 4 cut(s) 89, 319, 508, 1079
TaiI ACGT 3 cut(s) 141, 809, 849
TaqI TCGA 6 cut(s) 389, 409, 1016, 1353, 1429, 1682
TatI WGTACW 3 cut(s) 685, 1386, 1735
TfiI GAWTC 2 cut(s) 734, 1684
Tru1I TTAA 6 cut(s) 473, 495, 594, 671, 876, 1406
Tru9I TTAA 6 cut(s) 473, 495, 594, 671, 876, 1406
TscAI CASTG 3 cut(s) 248, 343, 708
TseI GCWGC 1 cut(s) 741
TspDTI ATGAA 8 cut(s) 45, 265, 500, 528, 558, 1448, 1559, 1567
TspGWI ACGGA 2 cut(s) 91, 197
TspRI CASTG 3 cut(s) 248, 343, 708
Vha464I CTTAAG 2 cut(s) 875, 1405
VpaK11BI GGWCC 3 cut(s) 1160, 1701, 1728
XapI RAATTY 7 cut(s) 385, 960, 987, 1004, 1425, 1435, 1574
XbaI TCTAGA 3 cut(s) 764, 1121, 1439
XceI RCATGY 1 cut(s) 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 843
XmnI GAANNNNTTC 1 cut(s) 1435
XspI CTAG 8 cut(s) 647, 731, 765, 770, 1073, 1122, 1440, 1583
ZrmI AGTACT 2 cut(s) 1388, 1737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.