RLG00000022349

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020125
Physical Location & Seq
Forward (+)
82745209 .. 82746061
853 bp
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UTR
Exon/CDS
Intron
RLM00000022349

Sequence Viewer

Length: 360 bp
ATGTGTGAAGAAGACATCCCTAAGACGGCATTCCATACTCATAAGGGACATTATGAGTTCTTGGTGATACCTTTTGGCCTTTCCAACGCCCCATCCACATTTCAAGCCTTGATGAATGTCATTTTCAAGCCAATTTTACGGAGGTTTGTCTTAGTCTTCTTTGATGATAGCTTGGTTTATAGCAAAGATCTCAATTCTCACCTTGAGCATCTTAAAGTGGTATTTGAGATCTTACTTGAGCATGGATTAAAAGTTAAGTTCTCTAAGTGTTCTTTTGCTCAAAACCATGTTAAATATTTGGGTCATGTAGTTTCTGCAAAGTGTGTTGCAATGGCCCTGATAAGGTTCAGTGTATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

120

Amino Acids

13.67

Weight (kDa)

8.83

Isoelectric Point (pI)

29.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 5 - 103 6.2e-25 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020125)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19792 FvH4_4g07791
pyrus_communis pycom13g26120 pycom16g15860
rosa_laevigata RLG00000022349
rosa_roxburghii Rroxscaffold_3G00238350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 2 cut(s) 25, 342
AgsI TTSAA 2 cut(s) 104, 127
AluBI AGCT 1 cut(s) 171
AluI AGCT 1 cut(s) 171
AoxI GGCC 2 cut(s) 76, 333
AspS9I GGNCC 1 cut(s) 334
AsuHPI GGTGA 2 cut(s) 76, 191
BbsI GAAGAC 2 cut(s) 18, 148
BccI CCATC 1 cut(s) 100
BceAI ACGGC 1 cut(s) 42
BglII AGATCT 2 cut(s) 187, 228
BmgT120I GGNCC 1 cut(s) 334
BmsI GCATC 1 cut(s) 217
BpiI GAAGAC 2 cut(s) 18, 148
BpuEI CTTGAG 2 cut(s) 224, 257
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 342
Bse3DI GCAATG 1 cut(s) 336
BseGI GGATG 2 cut(s) 15, 92
BseLI CCNNNNNNNGG 2 cut(s) 25, 342
BseMI GCAATG 1 cut(s) 336
BshFI GGCC 2 cut(s) 78, 335
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 25, 342
BsmFI GGGAC 1 cut(s) 60
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 2 cut(s) 78, 335
Bsp143I GATC 2 cut(s) 187, 228
BspANI GGCC 2 cut(s) 78, 335
BsrDI GCAATG 1 cut(s) 336
BssMI GATC 2 cut(s) 187, 228
BstDEI CTNAG 3 cut(s) 21, 151, 264
BstF5I GGATG 2 cut(s) 15, 92
BstKTI GATC 2 cut(s) 190, 231
BstMBI GATC 2 cut(s) 187, 228
BstV2I GAAGAC 2 cut(s) 18, 148
BstX2I RGATCY 2 cut(s) 187, 228
BstYI RGATCY 2 cut(s) 187, 228
BsuRI GGCC 2 cut(s) 78, 335
BtsCI GGATG 2 cut(s) 15, 92
BtsIMutI CAGTG 1 cut(s) 355
Cfr13I GGNCC 1 cut(s) 334
CviAII CATG 3 cut(s) 242, 287, 305
CviJI RGCY 5 cut(s) 78, 107, 130, 171, 335
CviKI_1 RGCY 5 cut(s) 78, 107, 130, 171, 335
DdeI CTNAG 3 cut(s) 21, 151, 264
DpnI GATC 2 cut(s) 189, 230
DpnII GATC 2 cut(s) 187, 228
FaeI CATG 3 cut(s) 245, 290, 308
FaiI YATR 7 cut(s) 36, 42, 54, 180, 243, 288, 306
FaqI GGGAC 1 cut(s) 60
FatI CATG 3 cut(s) 241, 286, 304
FokI GGATG 2 cut(s) 2, 79
HaeIII GGCC 2 cut(s) 78, 335
Hin1II CATG 3 cut(s) 245, 290, 308
HphI GGTGA 2 cut(s) 76, 191
HpyCH4V TGCA 2 cut(s) 317, 329
HpyF3I CTNAG 3 cut(s) 21, 151, 264
Hsp92II CATG 3 cut(s) 245, 290, 308
Kzo9I GATC 2 cut(s) 187, 228
LpnPI CCDG 1 cut(s) 350
LweI GCATC 1 cut(s) 217
MalI GATC 2 cut(s) 189, 230
MboI GATC 2 cut(s) 187, 228
MboII GAAGA 3 cut(s) 20, 23, 148
MflI RGATCY 2 cut(s) 187, 228
MluCI AATT 2 cut(s) 132, 193
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 1 cut(s) 135
MseI TTAA 4 cut(s) 213, 248, 255, 291
Mva1269I GAATGC 1 cut(s) 29
NdeII GATC 2 cut(s) 187, 228
NlaIII CATG 3 cut(s) 245, 290, 308
PctI GAATGC 1 cut(s) 29
PspPI GGNCC 1 cut(s) 334
PsuI RGATCY 2 cut(s) 187, 228
SaqAI TTAA 4 cut(s) 213, 248, 255, 291
Sau3AI GATC 2 cut(s) 187, 228
Sau96I GGNCC 1 cut(s) 334
SetI ASST 5 cut(s) 73, 146, 173, 204, 347
SfaNI GCATC 1 cut(s) 217
SmlI CTYRAG 2 cut(s) 203, 236
SmoI CTYRAG 2 cut(s) 203, 236
Sse9I AATT 2 cut(s) 132, 193
SspI AATATT 1 cut(s) 296
TasI AATT 2 cut(s) 132, 193
Tru1I TTAA 4 cut(s) 213, 248, 255, 291
Tru9I TTAA 4 cut(s) 213, 248, 255, 291
TscAI CASTG 1 cut(s) 355
TspDTI ATGAA 1 cut(s) 128
TspGWI ACGGA 1 cut(s) 154
TspRI CASTG 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.