pycom14g00300

Plant phosphoribosyltransferase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0015332
Physical Location & Seq
Reverse (-)
170179 .. 176502
6324 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4005 bp
ATGGCTACTATACGGAAACTGATAGTTGAGGTTGTCGATGCTCGAAATCTGTCGCCGAAAGATGGGCACGGCACGTCGAGCCCGTACGTGGTGATGGACTACTACGGGCAGCGGAAGAGGACGCAGACAAAGATACGAGACCTAAACCCCGTGTGGAACGAGTTGCTCGAGTTCAACGTTGTCAAGCCTTCCGATGTTTTTGGGGATGTGCTGGAGCTCGATGTCCACCACGACAAGAACTACGGACCCACCCGGAGGAACAACTTCCTCGGGAGGATCCGACTGAGTTCCACTCAGTTTGTCAAGAAAGGCGAGGAGGCTTTGATTTATTTCCCATTGGAGAAGAAGAGCCTCTTTAGTTGGATTCAAGGTGAAATTGGCTTGAAGATTTATTACGTCGATGAGGTTGTCCCGCCGCCTGCACCGCCAACACCGCCGCCTCAAGTGGAGGAGCCAAAGGCTGAAACTGCACCGCCTCCGTCGTCCGAGGCGCCGCCGCCACCAGAATCTGACTCAACCCCACCACCAGGCGAGAAGGAAACTAAGGCGGACCCACCACCCGAAGCAGAACAAGCACCGACACCGGAAGGTGAGAAGTCCGCCGAAGAACCACCGACTGAGGTGGTGCCACCACCAGAAACTGCAGCTCCTGAAGATGTACAATCTGATCCTCCACCGGGGCCGACGTCAACACCACCACCACCCACTGATGATGCAAATCATGATCAAGGGGACCCGCCGCCACCACCTGAAGCAGCCATGCAACGTCAACCCGAAATAGAGATGATGGCAGCCGCCGTATCGAAATCAGTTCCAGATTTCAAATTCCACGGAATCAGCGGCCCACAGCCAATGCCAAGGCCACCAGTAATGAACTATACGTCGCAGCTGGAGCCAACAGAAAGCATGTCGATGGATCGGCCGTCGTTTGATCTGGTGGAGAAGATGCACTACCTCTTCGTCCGAGTGGTGAAGGCGCGCTTCCTTCCCGCCAGCGGCAGTCCGATCGTTAAAATCTCCACTGCCAACCACCACATCACGTCCAAACCCGCCAGAAAAATCCAATGCTTCGAATGGGACCAGACCTTCGCCTTCGCCCGCCAATCCCCCGACCAATCCGACGCCTCCATCCTAGAAATTTCAGTGTGGGACCCGCCCATGTTTGACCCCGCGACCGCCTCCGAGGTTGCCTCCGGGCATCACTTCCTCGGTGGAATCTGTTTCGACGTGACGGAAATTCACCTGAGAGACCCGCCCGACAGCCCCTTGGCTCCGCAGTGGTACAGGCTGGAGGGTGGCGGGGCCCGCATGAACGGCGACCTGATGCTCGCCACGTGGATGGGAACCCAGGCGGACGATTCGTTTCCCGACGCGTGGAAGACCGATACAGCCGGTAACCCAAACGCGCGAGCCAAAGTCTATCAATCGCCGAAGCTGTGGTATCTCCGAGCAACAGTCCTCGAAGCCCAAGACGTTCTCCCCATGACGCCGTCGTTGACAGAGTCATCTGTCCAAGTCAAAGCCCAGCTCGGATTCCAGTCCCTCAAGACCGACCCCGCCGTCACCCAAAACGGCACGGTGTCGTGGAACCAAGACTTGATGTTCGTAGCCGCGGAGCCGTTCAGCGATCGTTTAATTTTCACTGTAGAGAATCGGCTAGCGAAAGCACCGGTCACATTGGGGGTGGCGACAATACCCCTTACCGCCATCGAACGACGCGTCGACGACCGCAAAGTGGCTTCGAGATGGTTCACATTGGAGAGCCCAGGCGAGGAAGACCAGAAGAAGAGGACGGCCTACAGAGGCAGAATGCACCTGCGCCTCTGTTTCGACGGCGGGTATCACGTGATGGACGAGGCGGCGTATGTGTGCAGCGACTACCGGCCCACCGCGAGGCAGCTCTGGAAGCCGCCGCTGGGCACCGTCCAGCTCGGCGTCATCGGGTGCAAGAACTTGATACCTGTCAAGACGGTGAGCGGTAAGGGATGTACGGATGCGTATTGCGTGGCAAAATATGGATCCAAATGGGTACGGACACGGACCGTGTGCGATAGCTTGGAGCCCAAATGGAACGAGCAGTATACGTTTAAAGTATACGATCCGTGTACGGTGTTGAGCATCGGCGTTTTTGATAGCTCAGGACCGGGATTCGAAATCGACCGCTCGACAGATGCCACGCGTCCCGACTTTCGTATCGGGAAGATCCGCGTGCGTATTTCGACGCTGGCTACGGGTAGAGTATACAGAAATACGTATCCGCTGTTGGTTTTGTCTCCCGCCGGTTTGAAAAAAATGGGGGAGGTGCAGGTGGCCATACGGTTTGTTCGAGTGTGTCCGACGTTGGATTTGATCCACGCGTACTCGCAGCCTTCACTGCCATTGATGCACCACATAAAGCCGCTAGGTGTGATGCAACAGGACTTGCTGCGGAGGGCGGCGGTTAAGATCGTCGCCGCACACCTGTCTCGCTCCGAACCGCCGCTCGGGCGCGAGGTTGTAATTTATATGCTGGACGCAGACTCGCAGGGATTCAGCATGCGGAAAGTGCGCGCCAATTATTTCCGGATCATCAATGTCGTAGCAGGGGTTGTGGATGTCGTAGCGTGGATAAACGACACGCGTTCTTGGAAGAAACCAATGGCAACGATTCTTGTGCACGCGCTACTGGTGCTGCTTGTTTGGTTCCCGGATCTGATCGTGCCCACCCTGTTGTTTTACGTGTTTGTGATTGGTGCGTGGAACTACAGGTTTCGCTCTCGGGCCCCACTCCAGCACTTTGACCCGAAGCTCTCGCTGGCGGACAACGTTGACCGTGACGAGCTGGACGAGGAGTTTGATGCGGTGCCGAGCAACAGGCCGTATGAGGTGGTGCGGGCAAGGTACGACAAGCTTAGGATGTTGGGGGCACGCGTGCAGACCGTGCTGGGAGATTTTGCAGCGCAAGGGGAACGAGTTCAGGCGCTGGTGACGTGGCGGGATCCGCGTGCGACGGGGATCTTTGTGGTGTTGTGTTTGGTGGTGGCGATGATACTGTACGTGGTGCCGTCGAAGATGGTGGCGATGGCGTTTGGGTTCTATTATCTCCGTCATCCGATATTCCGTGATCGGATGCCGTCGCCGGCTTTGAACTTCATTCGACGGCTTCCGTCGTTGTCTGATCGCCTTTTGTTCTCTGGGGTCTGTAGCTGTGTTTGGTGTGAAGCTTGGACTTGGATGCTGGTGTCGGTGAGGTGTGGCTTTGTTTGGGGATCGAATTTGACTGGATCAACATGCATGGCTGCTATTTTGGTGGTTGCAGCTGGCTTTCTAATTGGGCGCTGCGTTACATTCTTGTGTAAATATTTGGAACAGGAAATGGCTAGGAATCCCTTCTTGTTGGGGTTTCTTTTAGTTTTCTGCATAGCTATTGCAGAAGCAGAATACATTAAATATAAAGATCCCAAACAACCATTGAATTCTCGAATCATTGATCTACTGAGCCGGATGACGTTGGAGGAAAAGATTGGCCAAATGGTGCAGATAGACTGCAGCATTGCTTCGCCTGAGGTGACGAAGAAGCACTTCGTTGAAAATTTGTTTTGTTCAGGAAGTATATTAAGAGGGGTTGTGAATGTTCCGCCACAAAAGGATTCTGCAGAAACTTGGATTAACGTGGTGAATGATCTTCAGAAGGGTTCTTTATCAACTCGGCTTGGAATTCCGTTGATTTTTGGTATCGATGCTGTTCATGACCACAATAATGTTTACAAAGCAACAATCTTTCCTCACAATGTTGGCCTTGGAGCTACCAGGCAAGTAGATATCTCTCCATTGCTAACGGATCCTGAACTTGTTAAAAGGATCGGAGCTGCAACTGCGCTTGAAGTTAGAGCTACAGACATTCCTTACGTCTTTGCACCTTGTATAGCGGTAAATAAAATAAACTGTACATATCCGATGATTGATATGAACACAAGTCAATTGATGCTGGCACTGAACTCCATATTGATTTTGTCCGTGCAGTGA

Protein Analysis

1335

Amino Acids

148.68

Weight (kDa)

6.59

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 106 3.9e-18 C2 domain
C2 PF00168 480 - 586 8.1e-08 C2 domain
C2 PF00168 640 - 754 1e-16 C2 domain
PRT_C PF08372 900 - 1056 2.1e-70 Plant phosphoribosyltransferase C-terminal
Glyco_hydro_3 PF00933 1163 - 1307 4.1e-17 Glycosyl hydrolase family 3 N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015332)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17980
fragaria_vesca FvH4_5g32500
malus_domestica MD14G1004500.v1.1
prunus_persica Prupe.7G007300_v2.0.a1
pyrus_communis pycom14g00300
rosa_chinensis RchiOBHm_Chr7g0229081
rosa_laevigata RLG00000001532
rosa_multiflora Rmu_co8189216.1_g000001 Rmu_sc0000271.1_g000019
rosa_roxburghii Rroxscaffold_3G00230740
rosa_rugosa Rorug07G0255500
rosa_samantha Rh7AG406700 Rh7BG386100 Rh7CG424400 Rh7DG401000
rosa_wichuraiana Rw7G033760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1824, 2296
AasI GACNNNNNNGTC 1 cut(s) 1559
AatII GACGTC 1 cut(s) 689
Acc36I ACCTGC 2 cut(s) 1824, 2296
AccB1I GGYRCC 5 cut(s) 490, 625, 1919, 2842, 3040
AccBSI CCGCTC 3 cut(s) 1977, 2163, 2482
AccI GTMKAC 4 cut(s) 1722, 2081, 2094, 2241
AccIII TCCGGA 1 cut(s) 2562
AclI AACGTT 2 cut(s) 177, 2805
AcoI YGGCCR 3 cut(s) 920, 2310, 3505
AcsI RAATTY 7 cut(s) 824, 1137, 1236, 3253, 3454, 3571, 3696
AcuI CTGAAG 3 cut(s) 672, 771, 3650
AcvI CACGTG 2 cut(s) 1335, 1846
AcyI GRCGYC 5 cut(s) 491, 686, 1122, 1487, 1935
AflIII ACRYGT 7 cut(s) 1371, 1717, 2177, 2355, 2618, 2718, 2908
AgeI ACCGGT 1 cut(s) 1669
AgsI TTSAA 9 cut(s) 175, 368, 385, 823, 2287, 3127, 3454, 3569, 3863
AjiI CACGTC 4 cut(s) 75, 1041, 1228, 2970
AjnI CCWGG 4 cut(s) 526, 1347, 1765, 3788
Alw21I GWGCWC 2 cut(s) 219, 2658
Alw26I GTCTC 4 cut(s) 132, 1242, 2277, 2469
Alw44I GTGCAC 1 cut(s) 2654
AlwNI CAGNNNCTG 4 cut(s) 509, 641, 650, 2962
Ama87I CYCGRG 4 cut(s) 167, 269, 2483, 2757
Aor13HI TCCGGA 1 cut(s) 2562
ApaI GGGCCC 2 cut(s) 1306, 2764
ApaLI GTGCAC 1 cut(s) 2654
ApoI RAATTY 7 cut(s) 824, 1137, 1236, 3253, 3454, 3571, 3696
AsiGI ACCGGT 1 cut(s) 1669
Asp700I GAANNNNTTC 4 cut(s) 263, 2952, 3368, 3560
AsuC2I CCSGG 5 cut(s) 253, 678, 1195, 2145, 2687
AsuII TTCGAA 2 cut(s) 1071, 2151
AsuNHI GCTAGC 1 cut(s) 1657
AvaI CYCGRG 4 cut(s) 167, 269, 2483, 2757
AvaII GGWCC 7 cut(s) 245, 550, 733, 1078, 1150, 2040, 2141
AxyI CCTNAGG 1 cut(s) 3543
BaeGI GKGCMC 7 cut(s) 69, 1306, 1922, 2658, 2703, 2764, 2908
BaeI ACNNNNGTAYC 2 cut(s) 2176, 2209
BalI TGGCCA 2 cut(s) 2312, 3507
BamHI GGATCC 4 cut(s) 276, 2018, 2977, 3820
BanI GGYRCC 5 cut(s) 490, 625, 1919, 2842, 3040
BanII GRGCYC 6 cut(s) 83, 219, 1306, 1766, 2064, 2764
BarI GAAGNNNNNNTAC 4 cut(s) 377, 409, 935, 967
BbrPI CACGTG 2 cut(s) 1335, 1846
BbsI GAAGAC 2 cut(s) 1385, 1782
Bbv12I GWGCWC 2 cut(s) 219, 2658
BcgI CGANNNNNNTGC 4 cut(s) 988, 1022, 2635, 2669
BciT130I CCWGG 4 cut(s) 528, 1349, 1767, 3790
BciVI GTATCC 1 cut(s) 2265
BclI TGATCA 1 cut(s) 724
BcnI CCSGG 5 cut(s) 253, 678, 1195, 2145, 2687
BcoDI GTCTC 4 cut(s) 132, 1242, 2277, 2469
BfaI CTAG 4 cut(s) 1133, 1658, 2402, 3360
BfmI CTRYAG 8 cut(s) 642, 1644, 1798, 2743, 3181, 3526, 3633, 3873
BfoI RGCGCY 3 cut(s) 494, 2963, 3319
BfuAI ACCTGC 2 cut(s) 1824, 2296
BfuI GTATCC 1 cut(s) 2265
BglI GCCNNNNNGGC 1 cut(s) 2485
Bme1390I CCNGG 9 cut(s) 253, 528, 678, 1195, 1349, 1767, 2145, 2687, 3790
Bme18I GGWCC 7 cut(s) 245, 550, 733, 1078, 1150, 2040, 2141
BmeT110I CYCGRG 4 cut(s) 167, 269, 2483, 2757
BmgBI CACGTC 4 cut(s) 75, 1041, 1228, 2970
BmrFI CCNGG 9 cut(s) 253, 528, 678, 1195, 1349, 1767, 2145, 2687, 3790
BmtI GCTAGC 1 cut(s) 1661
BpiI GAAGAC 2 cut(s) 1385, 1782
BplI GAGNNNNNCTC 4 cut(s) 277, 309, 1175, 1207
BpmI CTGGAG 4 cut(s) 233, 911, 1310, 2753
Bpu10I CCTNAGC 2 cut(s) 2137, 2891
Bpu14I TTCGAA 2 cut(s) 1071, 2151
BpuEI CTTGAG 2 cut(s) 426, 1529
BpuMI CCSGG 5 cut(s) 253, 678, 1195, 2145, 2687
Bsa29I ATCGAT 1 cut(s) 3717
BsaAI YACGTR 6 cut(s) 88, 1335, 1846, 2253, 2719, 3037
BsaBI GATNNNNATC 1 cut(s) 717
BsaHI GRCGYC 5 cut(s) 491, 686, 1122, 1487, 1935
BsaI GGTCTC 2 cut(s) 132, 1242
BsaWI WCCGGW 3 cut(s) 583, 1669, 2562
BsaXI ACNNNNNCTCC 2 cut(s) 631, 661
Bse118I RCCGGY 5 cut(s) 1391, 1669, 1881, 2279, 3118
Bse1I ACTGG 4 cut(s) 866, 1537, 2670, 3265
Bse21I CCTNAGG 1 cut(s) 3543
Bse3DI GCAATG 2 cut(s) 3531, 3809
Bse8I GATNNNNATC 1 cut(s) 717
BseAI TCCGGA 1 cut(s) 2562
BseBI CCWGG 4 cut(s) 528, 1349, 1767, 3790
BseCI ATCGAT 1 cut(s) 3717
BseJI GATNNNNATC 1 cut(s) 717
BseMI GCAATG 2 cut(s) 3531, 3809
BseMII CTCAG 7 cut(s) 275, 308, 609, 1235, 2151, 3467, 3534
BseNI ACTGG 4 cut(s) 866, 1537, 2670, 3265
BsePI GCGCGC 2 cut(s) 977, 2548
BseRI GAGGAG 3 cut(s) 329, 464, 2843
BseSI GKGCMC 7 cut(s) 69, 1306, 1922, 2658, 2703, 2764, 2908
BseX3I CGGCCG 1 cut(s) 920
BseYI CCCAGC 3 cut(s) 1524, 1915, 2923
BsgI GTGCAG 6 cut(s) 405, 453, 1891, 2324, 2933, 3536
Bsh1285I CGRYCG 6 cut(s) 923, 1008, 1176, 1630, 1729, 2161
BshNI GGYRCC 5 cut(s) 490, 625, 1919, 2842, 3040
BshTI ACCGGT 1 cut(s) 1669
BshVI ATCGAT 1 cut(s) 3717
BsiEI CGRYCG 6 cut(s) 923, 1008, 1176, 1630, 1729, 2161
BsiHKAI GWGCWC 2 cut(s) 219, 2658
BsiHKCI CYCGRG 4 cut(s) 167, 269, 2483, 2757
BsiWI CGTACG 1 cut(s) 84
BslFI GGGAC 6 cut(s) 395, 746, 1091, 1163, 1525, 2166
BsmAI GTCTC 4 cut(s) 132, 1242, 2277, 2469
BsmFI GGGAC 6 cut(s) 395, 746, 1091, 1163, 1525, 2166
BsmI GAATGC 1 cut(s) 1815
Bso31I GGTCTC 2 cut(s) 132, 1242
BsoBI CYCGRG 4 cut(s) 167, 269, 2483, 2757
Bsp119I TTCGAA 2 cut(s) 1071, 2151
Bsp120I GGGCCC 2 cut(s) 1302, 2760
Bsp13I TCCGGA 1 cut(s) 2562
Bsp1407I TGTACA 2 cut(s) 658, 3926
BspCNI CTCAG 7 cut(s) 276, 307, 610, 1236, 2150, 3468, 3535
BspDI ATCGAT 1 cut(s) 3717
BspEI TCCGGA 1 cut(s) 2562
BspHI TCATGA 2 cut(s) 721, 3727
BspMAI CTGCAG 3 cut(s) 646, 3530, 3637
BspMI ACCTGC 2 cut(s) 1824, 2296
BspOI GCTAGC 1 cut(s) 1661
BspQI GCTCTTC 1 cut(s) 341
BspT104I TTCGAA 2 cut(s) 1071, 2151
BspT107I GGYRCC 5 cut(s) 490, 625, 1919, 2842, 3040
BspTNI GGTCTC 2 cut(s) 132, 1242
BsrBI CCGCTC 3 cut(s) 1977, 2163, 2482
BsrDI GCAATG 2 cut(s) 3531, 3809
BsrFI RCCGGY 5 cut(s) 1391, 1669, 1881, 2279, 3118
BsrGI TGTACA 2 cut(s) 658, 3926
BsrI ACTGG 4 cut(s) 866, 1537, 2670, 3265
BssAI RCCGGY 5 cut(s) 1391, 1669, 1881, 2279, 3118
BssHII GCGCGC 2 cut(s) 977, 2548
BssNAI GTATAC 3 cut(s) 2082, 2095, 2242
BssNI GRCGYC 5 cut(s) 491, 686, 1122, 1487, 1935
BssT1I CCWWGG 3 cut(s) 857, 1266, 3778
Bst1107I GTATAC 3 cut(s) 2082, 2095, 2242
Bst2UI CCWGG 4 cut(s) 528, 1349, 1767, 3790
Bst6I CTCTTC 4 cut(s) 110, 341, 962, 1781
BstACI GRCGYC 5 cut(s) 491, 686, 1122, 1487, 1935
BstAPI GCANNNNNTGC 1 cut(s) 2920
BstAUI TGTACA 2 cut(s) 658, 3926
BstBAI YACGTR 6 cut(s) 88, 1335, 1846, 2253, 2719, 3037
BstBI TTCGAA 2 cut(s) 1071, 2151
BstDEI CTNAG 9 cut(s) 284, 294, 543, 618, 1244, 2137, 2891, 3476, 3543
BstDSI CCRYGG 2 cut(s) 829, 1611
BstEII GGTNACC 1 cut(s) 1394
BstH2I RGCGCY 3 cut(s) 494, 2963, 3319
BstMAI GTCTC 4 cut(s) 132, 1242, 2277, 2469
BstMCI CGRYCG 6 cut(s) 923, 1008, 1176, 1630, 1729, 2161
BstNI CCWGG 4 cut(s) 528, 1349, 1767, 3790
BstNSI RCATGY 3 cut(s) 910, 2539, 3273
BstPI GGTNACC 1 cut(s) 1394
BstSCI CCNGG 9 cut(s) 251, 526, 676, 1193, 1347, 1765, 2143, 2685, 3788
BstSFI CTRYAG 8 cut(s) 642, 1644, 1798, 2743, 3181, 3526, 3633, 3873
BstSLI GKGCMC 7 cut(s) 69, 1306, 1922, 2658, 2703, 2764, 2908
BstSNI TACGTA 1 cut(s) 2253
BstV2I GAAGAC 2 cut(s) 1385, 1782
BstX2I RGATCY 8 cut(s) 276, 2018, 2202, 2689, 2977, 2994, 3436, 3820
BstXI CCANNNNNNTGG 1 cut(s) 1339
BstYI RGATCY 8 cut(s) 276, 2018, 2202, 2689, 2977, 2994, 3436, 3820
BstZ17I GTATAC 3 cut(s) 2082, 2095, 2242
BstZI CGGCCG 1 cut(s) 920
Bsu15I ATCGAT 1 cut(s) 3717
Bsu36I CCTNAGG 1 cut(s) 3543
BsuI GTATCC 1 cut(s) 2265
BsuTUI ATCGAT 1 cut(s) 3717
BtgI CCRYGG 2 cut(s) 829, 1611
BtgZI GCGATG 2 cut(s) 3038, 3074
BtrI CACGTC 4 cut(s) 75, 1041, 1228, 2970
BtsI GCAGTG 3 cut(s) 1020, 1283, 2372
BtsIMutI CAGTG 7 cut(s) 705, 1020, 1149, 1283, 1641, 2372, 3971
BveI ACCTGC 2 cut(s) 1824, 2296
CaiI CAGNNNCTG 4 cut(s) 509, 641, 650, 2962
CciI TCATGA 2 cut(s) 721, 3727
Cfr10I RCCGGY 5 cut(s) 1391, 1669, 1881, 2279, 3118
Cfr42I CCGCGG 1 cut(s) 1614
ClaI ATCGAT 1 cut(s) 3717
CpoI CGGWCCG 1 cut(s) 2040
CspAI ACCGGT 1 cut(s) 1669
CspCI CAANNNNNGTGG 2 cut(s) 280, 315
CspI CGGWCCG 1 cut(s) 2040
DdeI CTNAG 9 cut(s) 284, 294, 543, 618, 1244, 2137, 2891, 3476, 3543
DinI GGCGCC 1 cut(s) 492
DraI TTTAAA 1 cut(s) 2089
DrdI GACNNNNNNGTC 1 cut(s) 1559
DseDI GACNNNNNNGTC 1 cut(s) 1559
EaeI YGGCCR 3 cut(s) 920, 2310, 3505
EagI CGGCCG 1 cut(s) 920
Eam1104I CTCTTC 4 cut(s) 110, 341, 962, 1781
EarI CTCTTC 4 cut(s) 110, 341, 962, 1781
EciI GGCGGA 5 cut(s) 563, 589, 1367, 2813, 3606
Ecl136II GAGCTC 1 cut(s) 217
EclXI CGGCCG 1 cut(s) 920
Eco105I TACGTA 1 cut(s) 2253
Eco130I CCWWGG 3 cut(s) 857, 1266, 3778
Eco24I GRGCYC 6 cut(s) 83, 219, 1306, 1766, 2064, 2764
Eco31I GGTCTC 2 cut(s) 132, 1242
Eco32I GATATC 1 cut(s) 3802
Eco47I GGWCC 7 cut(s) 245, 550, 733, 1078, 1150, 2040, 2141
Eco52I CGGCCG 1 cut(s) 920
Eco53kI GAGCTC 1 cut(s) 217
Eco57I CTGAAG 3 cut(s) 672, 771, 3650
Eco72I CACGTG 2 cut(s) 1335, 1846
Eco81I CCTNAGG 1 cut(s) 3543
Eco88I CYCGRG 4 cut(s) 167, 269, 2483, 2757
Eco91I GGTNACC 1 cut(s) 1394
EcoICRI GAGCTC 1 cut(s) 217
EcoO109I RGGNCCY 4 cut(s) 733, 1150, 1302, 2761
EcoO65I GGTNACC 1 cut(s) 1394
EcoRI GAATTC 2 cut(s) 3454, 3696
EcoRII CCWGG 4 cut(s) 526, 1347, 1765, 3788
EcoRV GATATC 1 cut(s) 3802
EcoT14I CCWWGG 3 cut(s) 857, 1266, 3778
EcoT22I ATGCAT 1 cut(s) 3275
EcoT38I GRGCYC 6 cut(s) 83, 219, 1306, 1766, 2064, 2764
EgeI GGCGCC 1 cut(s) 492
EheI GGCGCC 1 cut(s) 492
ErhI CCWWGG 3 cut(s) 857, 1266, 3778
FalI AAGNNNNNCTT 6 cut(s) 338, 370, 965, 997, 3545, 3577
FaqI GGGAC 6 cut(s) 395, 746, 1091, 1163, 1525, 2166
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 4 cut(s) 1722, 2081, 2094, 2241
FriOI GRGCYC 6 cut(s) 83, 219, 1306, 1766, 2064, 2764
FspBI CTAG 4 cut(s) 1133, 1658, 2402, 3360
GsaI CCCAGC 3 cut(s) 1528, 1919, 2927
GsuI CTGGAG 4 cut(s) 233, 911, 1310, 2753
HaeII RGCGCY 3 cut(s) 494, 2963, 3319
Hin1I GRCGYC 5 cut(s) 491, 686, 1122, 1487, 1935
HincII GTYRAC 5 cut(s) 690, 770, 1497, 1723, 2809
HindII GTYRAC 5 cut(s) 690, 770, 1497, 1723, 2809
HindIII AAGCTT 2 cut(s) 2888, 3201
HpyF3I CTNAG 9 cut(s) 284, 294, 543, 618, 1244, 2137, 2891, 3476, 3543
Hsp92I GRCGYC 5 cut(s) 491, 686, 1122, 1487, 1935
KasI GGCGCC 1 cut(s) 490
KflI GGGWCCC 2 cut(s) 733, 1150
Kpn2I TCCGGA 1 cut(s) 2562
KroI GCCGGC 1 cut(s) 3118
KroNI GCCGGC 1 cut(s) 3120
Ksp22I TGATCA 1 cut(s) 724
KspI CCGCGG 1 cut(s) 1614
LguI GCTCTTC 1 cut(s) 341
MaeI CTAG 4 cut(s) 1133, 1658, 2402, 3360
MaeIII GTNAC 8 cut(s) 1228, 1394, 1561, 1672, 2813, 2965, 3322, 3547
MbiI CCGCTC 3 cut(s) 1977, 2163, 2482
MfeI CAATTG 1 cut(s) 3959
MflI RGATCY 8 cut(s) 276, 2018, 2202, 2689, 2977, 2994, 3436, 3820
MlsI TGGCCA 2 cut(s) 2312, 3507
MluI ACGCGT 6 cut(s) 1371, 1717, 2177, 2355, 2618, 2908
MluNI TGGCCA 2 cut(s) 2312, 3507
Mly113I GGCGCC 1 cut(s) 491
MlyI GAGTC 3 cut(s) 506, 1511, 2513
MmeI TCCRAC 6 cut(s) 304, 341, 1143, 2322, 2360, 3471
Mox20I TGGCCA 2 cut(s) 2312, 3507
Mph1103I ATGCAT 1 cut(s) 3275
MroI TCCGGA 1 cut(s) 2562
MroNI GCCGGC 1 cut(s) 3118
MroXI GAANNNNTTC 4 cut(s) 263, 2952, 3368, 3560
MscI TGGCCA 2 cut(s) 2312, 3507
MseI TTAA 8 cut(s) 1011, 1634, 2088, 2442, 3426, 3596, 3648, 3834
MslI CAYNNNNRTG 4 cut(s) 911, 1337, 3331, 3738
Msp20I TGGCCA 2 cut(s) 2312, 3507
MspA1I CMGCKG 8 cut(s) 112, 840, 889, 996, 1613, 1915, 2260, 3299
MspR9I CCNGG 9 cut(s) 253, 528, 678, 1195, 1349, 1767, 2145, 2687, 3790
MunI CAATTG 1 cut(s) 3959
Mva1269I GAATGC 1 cut(s) 1815
MvaI CCWGG 4 cut(s) 528, 1349, 1767, 3790
NaeI GCCGGC 1 cut(s) 3120
NarI GGCGCC 1 cut(s) 491
NciI CCSGG 5 cut(s) 253, 678, 1195, 2145, 2687
NgoMIV GCCGGC 1 cut(s) 3118
NheI GCTAGC 1 cut(s) 1657
NmeAIII GCCGAG 3 cut(s) 1911, 2871, 3667
NmuCI GTSAC 6 cut(s) 1228, 1561, 1672, 2813, 2965, 3547
NsiI ATGCAT 1 cut(s) 3275
NspI RCATGY 3 cut(s) 910, 2539, 3273
NspV TTCGAA 2 cut(s) 1071, 2151
PaeI GCATGC 1 cut(s) 2539
PaeR7I CTCGAG 1 cut(s) 167
PagI TCATGA 2 cut(s) 721, 3727
PaqCI CACCTGC 2 cut(s) 1824, 2296
PauI GCGCGC 2 cut(s) 977, 2548
PciSI GCTCTTC 1 cut(s) 341
PcsI WCGNNNNNNNCGW 7 cut(s) 80, 165, 174, 1716, 1851, 2323, 2823
PctI GAATGC 1 cut(s) 1815
PdiI GCCGGC 1 cut(s) 3120
PdmI GAANNNNTTC 4 cut(s) 263, 2952, 3368, 3560
Pfl23II CGTACG 1 cut(s) 84
PflFI GACNNNGTC 2 cut(s) 1489, 1501
PfoI TCCNGGA 1 cut(s) 2685
PinAI ACCGGT 1 cut(s) 1669
Ple19I CGATCG 2 cut(s) 1008, 1630
PleI GAGTC 3 cut(s) 506, 1510, 2513
PluTI GGCGCC 1 cut(s) 494
PmaCI CACGTG 2 cut(s) 1335, 1846
PmlI CACGTG 2 cut(s) 1335, 1846
PpsI GAGTC 3 cut(s) 506, 1510, 2513
Ppu21I YACGTR 6 cut(s) 88, 1335, 1846, 2253, 2719, 3037
PpuMI RGGWCCY 2 cut(s) 733, 1150
Psp124BI GAGCTC 1 cut(s) 219
Psp1406I AACGTT 2 cut(s) 177, 2805
Psp5II RGGWCCY 2 cut(s) 733, 1150
Psp6I CCWGG 4 cut(s) 526, 1347, 1765, 3788
PspCI CACGTG 2 cut(s) 1335, 1846
PspEI GGTNACC 1 cut(s) 1394
PspFI CCCAGC 3 cut(s) 1524, 1915, 2923
PspGI CCWGG 4 cut(s) 526, 1347, 1765, 3788
PspLI CGTACG 1 cut(s) 84
PspOMI GGGCCC 2 cut(s) 1302, 2760
PspPPI RGGWCCY 2 cut(s) 733, 1150
PspXI VCTCGAGB 1 cut(s) 167
PstI CTGCAG 3 cut(s) 646, 3530, 3637
PstNI CAGNNNCTG 4 cut(s) 509, 641, 650, 2962
PsuI RGATCY 8 cut(s) 276, 2018, 2202, 2689, 2977, 2994, 3436, 3820
PsyI GACNNNGTC 2 cut(s) 1489, 1501
PteI GCGCGC 2 cut(s) 977, 2548
PvuI CGATCG 2 cut(s) 1008, 1630
PvuII CAGCTG 2 cut(s) 889, 3299
RseI CAYNNNNRTG 4 cut(s) 911, 1337, 3331, 3738
Rsr2I CGGWCCG 1 cut(s) 2040
RsrII CGGWCCG 1 cut(s) 2040
SacI GAGCTC 1 cut(s) 219
SacII CCGCGG 1 cut(s) 1614
SalI GTCGAC 1 cut(s) 1721
SapI GCTCTTC 1 cut(s) 341
SaqAI TTAA 8 cut(s) 1011, 1634, 2088, 2442, 3426, 3596, 3648, 3834
SchI GAGTC 3 cut(s) 506, 1511, 2513
ScrFI CCNGG 9 cut(s) 253, 528, 678, 1195, 1349, 1767, 2145, 2687, 3790
SfcI CTRYAG 8 cut(s) 642, 1644, 1798, 2743, 3181, 3526, 3633, 3873
SfoI GGCGCC 1 cut(s) 492
Sfr274I CTCGAG 1 cut(s) 167
Sfr303I CCGCGG 1 cut(s) 1614
SfuI TTCGAA 2 cut(s) 1071, 2151
SgrBI CCGCGG 1 cut(s) 1614
SgrDI CGTCGACG 1 cut(s) 1721
SinI GGWCC 7 cut(s) 245, 550, 733, 1078, 1150, 2040, 2141
SlaI CTCGAG 1 cut(s) 167
SmiMI CAYNNNNRTG 4 cut(s) 911, 1337, 3331, 3738
SmlI CTYRAG 3 cut(s) 167, 441, 1544
SmoI CTYRAG 3 cut(s) 167, 441, 1544
SnaBI TACGTA 1 cut(s) 2253
SphI GCATGC 1 cut(s) 2539
SspDI GGCGCC 1 cut(s) 490
SspI AATATT 1 cut(s) 3341
SspMI CTAG 4 cut(s) 1133, 1658, 2402, 3360
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 9 cut(s) 251, 526, 676, 1193, 1347, 1765, 2143, 2685, 3788
StyI CCWWGG 3 cut(s) 857, 1266, 3778
TaqII GACCGA 2 cut(s) 1397, 1565
TatI WGTACW 2 cut(s) 658, 3926
Tru1I TTAA 8 cut(s) 1011, 1634, 2088, 2442, 3426, 3596, 3648, 3834
Tru9I TTAA 8 cut(s) 1011, 1634, 2088, 2442, 3426, 3596, 3648, 3834
TscAI CASTG 7 cut(s) 712, 1027, 1149, 1283, 1648, 2379, 3978
TseFI GTSAC 6 cut(s) 1228, 1561, 1672, 2813, 2965, 3547
Tsp45I GTSAC 6 cut(s) 1228, 1561, 1672, 2813, 2965, 3547
TspDTI ATGAA 5 cut(s) 887, 1325, 3121, 3716, 3962
TspRI CASTG 7 cut(s) 712, 1027, 1149, 1283, 1648, 2379, 3978
Tth111I GACNNNGTC 2 cut(s) 1489, 1501
VneI GTGCAC 1 cut(s) 2654
VpaK11BI GGWCC 7 cut(s) 245, 550, 733, 1078, 1150, 2040, 2141
XapI RAATTY 7 cut(s) 824, 1137, 1236, 3253, 3454, 3571, 3696
XceI RCATGY 3 cut(s) 910, 2539, 3273
XhoI CTCGAG 1 cut(s) 167
XmiI GTMKAC 4 cut(s) 1722, 2081, 2094, 2241
XmnI GAANNNNTTC 4 cut(s) 263, 2952, 3368, 3560
XspI CTAG 4 cut(s) 1133, 1658, 2402, 3360
ZraI GACGTC 1 cut(s) 687
Zsp2I ATGCAT 1 cut(s) 3275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.