pycom14g06290

RRP12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011076
Physical Location & Seq
Forward (+)
6149132 .. 6152176
3045 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1374 bp
ATGCATGTGATCTGTTGGGATTACTACCTGCTTTCTGCCAATCTTCCAACCGATATAGGCCACAAGTTTCGGCCGTTGGCTGGGATTTTAGTAACATTTCTTCAGGACTCCTTAATGCATGAAAATACTGCAGTTGCCTCGCAGGTTATTGTGAATCAGAACAAAAGTGTACTTGATCAAAAAGATGGTGCTGGTGACACAAGTTCCTATGCAGTAAACAAATCTTTACTAGAGTTCTGCAACATACCCACTTATTCAAAGAAAACTGCTACCAGAAACATTAAGGCTTTGACATCATGTTCTTCTGAGCTGCTTATAGCTCGCACAGACGTATTTCTTGATTCACCCCCTGAGAAGCGTTCATATTTAAAGGATGCCATAGGATGCTTGGCTTCAGTAACTGATTCTTCCAATGCCAAGAAGATTTTCATGTCGTTGCTTAGGAAGTTCCAGTTCAAAGATGGTAGTCATGAGCTTGAAAAGGTCGAGAGTCAGACTGATGCATTGGCCGGTGAAGAACAACACAACCTAAGTACTAGAGAAAAAGATGCTCTGCGATGCGTGATGATGGAATTAGCGTCTTCTTTTGTTAAAGGAGCTAAGGAGGATCTTATTTATTTAATCTATACCTTTGCTAAAGATACTTTACAGAACGATGATGAGATCGCCATTAGTGAATCATACCATACATTGAGCAGAATTCTAGAGGAGCATGATTGGTTCTGTTCGTCTGGCTATGCTGAATTGATTTATCTGCTACTTGGTTTGAGATCTCCCGTTGATATCATATCTCTAAGGAGTCGTTTTGCTAGTTTCCAGAGTTTAATGGTTCATACTTTGAAGATAGACTCAGAGGTGGAAAATTCAAAATGGTTTCTTATTCTCAATGAGATCGTACTCAAGTTAAAAGATGTACAACTTTCTGGATACATGACAATGGATCTGTTAATAATATTTAGTGTTTCTGGATACATGACAATGGAGAAAGCTGTTGAGATTTTTGATGAGCTTAAGGAGAGATGTTTGGTTTATGATGGGGTCGTGAATTCGACGTTTATGGATGGGTTTTTCAACTGGGGGAAGGAGAAGGAGGCGATGGAGTCGTACAAGTCCTTGCTTGATAGGCAGTTGAGGATGGCCCCGGCAACTTGTAATGTTCTCTTGGAGGTATTGCTGAAGTACGGGAAGAAGAAGGAAGCCTGGGATTTGTTCGACCAGATGTTGGATAATCACACCCCGCCAAATTTCCAGGCTGTGAATTCGGAAACCTTTACCATAATGGTTAATGAGTGTTTTAAGCTTGGAAAGTGCGACGGGGCGCTTACCACGTTCACGGCCATAGGACACAACTTGCATGTGTATGAGTATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

458

Amino Acids

51.88

Weight (kDa)

5.28

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 383 - 433 5.9e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011076)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23540
fragaria_vesca FvH4_6g24030 FvH4_6g24030 FvH4_6g24030 FvH4_6g24030
malus_domestica MD12G1075000.v1.1
prunus_persica Prupe.7G058600_v2.0.a1
pyrus_communis pycom12g06460 pycom14g06290
rosa_chinensis RchiOBHm_Chr3g0482611
rosa_laevigata RLG00000023336
rosa_multiflora Rmu_co8265035.1_g000001 Rmu_sc0015494.1_g000006
rosa_roxburghii Rroxscaffold_6G00399070
rosa_rugosa Rorug03G0197600
rosa_samantha Rh3AG248700 Rh3BG284200 Rh3CG281700 Rh3DG277900
rosa_wichuraiana Rw3G022460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 36, 133
AccB7I CCANNNNNTGG 1 cut(s) 1222
AciI CCGC 1 cut(s) 1238
AclWI GGATC 2 cut(s) 615, 948
AcoI YGGCCR 3 cut(s) 71, 507, 1335
AcsI RAATTY 5 cut(s) 699, 862, 1045, 1243, 1258
AcuI CTGAAG 3 cut(s) 86, 378, 1196
AfaI GTAC 6 cut(s) 171, 535, 897, 915, 1106, 1181
AfiI CCNNNNNNNGG 2 cut(s) 80, 1222
AflII CTTAAG 1 cut(s) 1010
AgsI TTSAA 6 cut(s) 258, 457, 479, 841, 867, 1072
AjnI CCWGG 2 cut(s) 1199, 1248
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 7 cut(s) 310, 320, 475, 599, 989, 1009, 1300
AluI AGCT 7 cut(s) 310, 320, 475, 599, 989, 1009, 1300
AlwI GGATC 2 cut(s) 615, 948
AlwNI CAGNNNCTG 1 cut(s) 401
AoxI GGCC 5 cut(s) 58, 71, 507, 1137, 1335
ApeKI GCWGC 1 cut(s) 310
ApoI RAATTY 5 cut(s) 699, 862, 1045, 1243, 1258
Asp700I GAANNNNTTC 1 cut(s) 425
AspLEI GCGC 1 cut(s) 1321
AspS9I GGNCC 1 cut(s) 1138
AsuC2I CCSGG 1 cut(s) 1142
AsuHPI GGTGA 3 cut(s) 206, 336, 524
BarI GAAGNNNNNNTAC 2 cut(s) 84, 116
BbsI GAAGAC 1 cut(s) 573
BbvI GCAGC 1 cut(s) 297
BccI CCATC 7 cut(s) 179, 455, 562, 1028, 1055, 1090, 1129
BceAI ACGGC 2 cut(s) 58, 1350
BcgI CGANNNNNNTGC 2 cut(s) 120, 154
BciT130I CCWGG 2 cut(s) 1201, 1250
BciVI GTATCC 2 cut(s) 920, 962
BclI TGATCA 1 cut(s) 175
BcnI CCSGG 1 cut(s) 1142
BfaI CTAG 5 cut(s) 230, 537, 704, 810, 1372
BfmI CTRYAG 1 cut(s) 129
BfoI RGCGCY 1 cut(s) 1322
BfrI CTTAAG 1 cut(s) 1010
BfuAI ACCTGC 2 cut(s) 36, 133
BfuI GTATCC 2 cut(s) 920, 962
BglII AGATCT 1 cut(s) 770
BisI GCNGC 1 cut(s) 311
BlsI GCNGC 1 cut(s) 312
BmcAI AGTACT 1 cut(s) 535
Bme1390I CCNGG 3 cut(s) 1142, 1201, 1250
BmgT120I GGNCC 1 cut(s) 1138
BmiI GGNNCC 1 cut(s) 1140
BmrFI CCNGG 3 cut(s) 1142, 1201, 1250
BmrI ACTGGG 1 cut(s) 1084
BmsI GCATC 5 cut(s) 364, 374, 490, 538, 548
BmuI ACTGGG 1 cut(s) 1084
BpiI GAAGAC 1 cut(s) 573
Bpu10I CCTNAGC 2 cut(s) 440, 600
BpuEI CTTGAG 1 cut(s) 884
BpuMI CCSGG 1 cut(s) 1142
BsaJI CCNNGG 2 cut(s) 1140, 1200
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 1222
Bse118I RCCGGY 1 cut(s) 509
Bse1I ACTGG 2 cut(s) 451, 1079
BseBI CCWGG 2 cut(s) 1201, 1250
BseDI CCNNGG 2 cut(s) 1140, 1200
BseGI GGATG 4 cut(s) 379, 389, 1066, 1140
BseLI CCNNNNNNNGG 2 cut(s) 80, 1222
BseMII CTCAG 3 cut(s) 297, 342, 864
BseNI ACTGG 2 cut(s) 451, 1079
BseRI GAGGAG 1 cut(s) 722
BseX3I CGGCCG 1 cut(s) 71
BseXI GCAGC 1 cut(s) 297
BseYI CCCAGC 1 cut(s) 80
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 5 cut(s) 60, 73, 509, 1139, 1337
BsiEI CGRYCG 1 cut(s) 74
BsiSI CCGG 2 cut(s) 510, 1142
BslI CCNNNNNNNGG 2 cut(s) 80, 1222
BsnI GGCC 5 cut(s) 60, 73, 509, 1139, 1337
Bsp1407I TGTACA 1 cut(s) 913
Bsp143I GATC 7 cut(s) 9, 175, 607, 663, 770, 891, 940
BspACI CCGC 1 cut(s) 1238
BspANI GGCC 5 cut(s) 60, 73, 509, 1139, 1337
BspCNI CTCAG 3 cut(s) 298, 343, 863
BspHI TCATGA 1 cut(s) 469
BspLI GGNNCC 1 cut(s) 1140
BspMAI CTGCAG 1 cut(s) 133
BspMI ACCTGC 2 cut(s) 36, 133
BspPI GGATC 2 cut(s) 615, 948
BspTI CTTAAG 1 cut(s) 1010
BsrFI RCCGGY 1 cut(s) 509
BsrGI TGTACA 1 cut(s) 913
BsrI ACTGG 2 cut(s) 451, 1079
BssAI RCCGGY 1 cut(s) 509
BssECI CCNNGG 2 cut(s) 1140, 1200
BssMI GATC 7 cut(s) 9, 175, 607, 663, 770, 891, 940
Bst2UI CCWGG 2 cut(s) 1201, 1250
BstAFI CTTAAG 1 cut(s) 1010
BstAUI TGTACA 1 cut(s) 913
BstC8I GCNNGC 1 cut(s) 322
BstDEI CTNAG 7 cut(s) 306, 351, 440, 530, 600, 794, 850
BstF5I GGATG 4 cut(s) 379, 389, 1066, 1140
BstH2I RGCGCY 1 cut(s) 1322
BstHHI GCGC 1 cut(s) 1321
BstKTI GATC 7 cut(s) 12, 178, 610, 666, 773, 894, 943
BstMBI GATC 7 cut(s) 9, 175, 607, 663, 770, 891, 940
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 1 cut(s) 1123
BstNI CCWGG 2 cut(s) 1201, 1250
BstNSI RCATGY 2 cut(s) 8, 1358
BstSCI CCNGG 3 cut(s) 1140, 1199, 1248
BstSFI CTRYAG 1 cut(s) 129
BstV1I GCAGC 1 cut(s) 297
BstV2I GAAGAC 1 cut(s) 573
BstX2I RGATCY 3 cut(s) 607, 770, 940
BstYI RGATCY 3 cut(s) 607, 770, 940
BstZI CGGCCG 1 cut(s) 71
BsuI GTATCC 2 cut(s) 920, 962
BsuRI GGCC 5 cut(s) 60, 73, 509, 1139, 1337
BtgZI GCGATG 2 cut(s) 571, 1109
BtsCI GGATG 4 cut(s) 379, 389, 1066, 1140
BveI ACCTGC 2 cut(s) 36, 133
Cac8I GCNNGC 1 cut(s) 322
CaiI CAGNNNCTG 1 cut(s) 401
CciI TCATGA 1 cut(s) 469
CfoI GCGC 1 cut(s) 1321
Cfr10I RCCGGY 1 cut(s) 509
Cfr13I GGNCC 1 cut(s) 1138
CseI GACGC 1 cut(s) 567
Csp6I GTAC 6 cut(s) 170, 534, 896, 914, 1105, 1180
CviAII CATG 9 cut(s) 5, 119, 297, 430, 470, 713, 931, 973, 1355
CviQI GTAC 6 cut(s) 170, 534, 896, 914, 1105, 1180
DdeI CTNAG 7 cut(s) 306, 351, 440, 530, 600, 794, 850
DpnI GATC 7 cut(s) 11, 177, 609, 665, 772, 893, 942
DpnII GATC 7 cut(s) 9, 175, 607, 663, 770, 891, 940
DraI TTTAAA 1 cut(s) 369
EaeI YGGCCR 3 cut(s) 71, 507, 1335
EagI CGGCCG 1 cut(s) 71
EclXI CGGCCG 1 cut(s) 71
Eco32I GATATC 1 cut(s) 784
Eco52I CGGCCG 1 cut(s) 71
Eco57I CTGAAG 3 cut(s) 86, 378, 1196
EcoRI GAATTC 3 cut(s) 699, 1045, 1258
EcoRII CCWGG 2 cut(s) 1199, 1248
EcoRV GATATC 1 cut(s) 784
EcoT22I ATGCAT 3 cut(s) 6, 120, 505
FaeI CATG 9 cut(s) 8, 122, 300, 433, 473, 716, 934, 976, 1358
FatI CATG 9 cut(s) 4, 118, 296, 429, 469, 712, 930, 972, 1354
FauI CCCGC 1 cut(s) 1245
FbaI TGATCA 1 cut(s) 175
Fnu4HI GCNGC 1 cut(s) 311
FokI GGATG 4 cut(s) 386, 396, 1073, 1147
Fsp4HI GCNGC 1 cut(s) 311
FspBI CTAG 5 cut(s) 230, 537, 704, 810, 1372
GlaI GCGC 1 cut(s) 1320
GluI GCNGC 1 cut(s) 311
GsaI CCCAGC 1 cut(s) 84
HaeII RGCGCY 1 cut(s) 1322
HaeIII GGCC 5 cut(s) 60, 73, 509, 1139, 1337
HapII CCGG 2 cut(s) 510, 1142
HgaI GACGC 1 cut(s) 567
HhaI GCGC 1 cut(s) 1321
Hin1II CATG 9 cut(s) 8, 122, 300, 433, 473, 716, 934, 976, 1358
Hin6I GCGC 1 cut(s) 1319
HinP1I GCGC 1 cut(s) 1319
HindIII AAGCTT 1 cut(s) 1298
HinfI GANTC 9 cut(s) 107, 154, 341, 404, 490, 677, 799, 848, 1100
HpaII CCGG 2 cut(s) 510, 1142
HphI GGTGA 3 cut(s) 206, 336, 524
Hpy166II GTNNAC 3 cut(s) 170, 217, 1332
Hpy188I TCNGA 5 cut(s) 159, 307, 495, 853, 1264
Hpy188III TCNNGA 9 cut(s) 104, 338, 470, 487, 704, 817, 924, 966, 1042
Hpy8I GTNNAC 3 cut(s) 170, 217, 1332
Hpy99I CGWCG 2 cut(s) 1054, 1316
HpyAV CCTTC 3 cut(s) 1075, 1081, 1186
HpyCH4IV ACGT 3 cut(s) 330, 1052, 1328
HpyCH4V TGCA 7 cut(s) 4, 118, 131, 212, 240, 503, 1354
HpyF10VI GCNNNNNNNGC 1 cut(s) 1123
HpyF3I CTNAG 7 cut(s) 306, 351, 440, 530, 600, 794, 850
HpySE526I ACGT 3 cut(s) 330, 1052, 1328
Hsp92II CATG 9 cut(s) 8, 122, 300, 433, 473, 716, 934, 976, 1358
HspAI GCGC 1 cut(s) 1319
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 7 cut(s) 9, 175, 607, 663, 770, 891, 940
LmnI GCTCC 2 cut(s) 596, 709
Lsp1109I GCAGC 1 cut(s) 297
LweI GCATC 5 cut(s) 364, 374, 490, 538, 548
MaeI CTAG 5 cut(s) 230, 537, 704, 810, 1372
MaeII ACGT 3 cut(s) 330, 1052, 1328
MaeIII GTNAC 3 cut(s) 91, 194, 397
MalI GATC 7 cut(s) 11, 177, 609, 665, 772, 893, 942
MboI GATC 7 cut(s) 9, 175, 607, 663, 770, 891, 940
MflI RGATCY 3 cut(s) 607, 770, 940
MluCI AATT 7 cut(s) 572, 699, 743, 862, 1045, 1243, 1258
MlyI GAGTC 5 cut(s) 101, 499, 808, 842, 1109
MmeI TCCRAC 2 cut(s) 71, 1203
MnlI CCTC 7 cut(s) 148, 598, 700, 847, 1084, 1125, 1159
Mph1103I ATGCAT 3 cut(s) 6, 120, 505
MroXI GAANNNNTTC 1 cut(s) 425
MslI CAYNNNNRTG 3 cut(s) 935, 977, 1359
MspCI CTTAAG 1 cut(s) 1010
MspI CCGG 2 cut(s) 510, 1142
MspR9I CCNGG 3 cut(s) 1142, 1201, 1250
MvaI CCWGG 2 cut(s) 1201, 1250
MwoI GCNNNNNNNGC 1 cut(s) 1123
NciI CCSGG 1 cut(s) 1142
NdeII GATC 7 cut(s) 9, 175, 607, 663, 770, 891, 940
NlaIII CATG 9 cut(s) 8, 122, 300, 433, 473, 716, 934, 976, 1358
NlaIV GGNNCC 1 cut(s) 1140
NmuCI GTSAC 1 cut(s) 194
NsiI ATGCAT 3 cut(s) 6, 120, 505
NspI RCATGY 2 cut(s) 8, 1358
PagI TCATGA 1 cut(s) 469
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 4 cut(s) 154, 341, 404, 677
PflMI CCANNNNNTGG 1 cut(s) 1222
PkrI GCNGC 1 cut(s) 312
PleI GAGTC 5 cut(s) 101, 498, 807, 842, 1108
PpsI GAGTC 5 cut(s) 101, 498, 807, 842, 1108
Psp6I CCWGG 2 cut(s) 1199, 1248
PspFI CCCAGC 1 cut(s) 80
PspGI CCWGG 2 cut(s) 1199, 1248
PspN4I GGNNCC 1 cut(s) 1140
PspPI GGNCC 1 cut(s) 1138
PstI CTGCAG 1 cut(s) 133
PstNI CAGNNNCTG 1 cut(s) 401
PsuI RGATCY 3 cut(s) 607, 770, 940
RsaI GTAC 6 cut(s) 171, 535, 897, 915, 1106, 1181
RsaNI GTAC 6 cut(s) 170, 534, 896, 914, 1105, 1180
RseI CAYNNNNRTG 3 cut(s) 935, 977, 1359
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 7 cut(s) 9, 175, 607, 663, 770, 891, 940
Sau96I GGNCC 1 cut(s) 1138
ScaI AGTACT 1 cut(s) 535
SchI GAGTC 5 cut(s) 101, 499, 808, 842, 1109
ScrFI CCNGG 3 cut(s) 1142, 1201, 1250
SfaNI GCATC 5 cut(s) 364, 374, 490, 538, 548
SfcI CTRYAG 1 cut(s) 129
SmiMI CAYNNNNRTG 3 cut(s) 935, 977, 1359
SmlI CTYRAG 2 cut(s) 899, 1010
SmoI CTYRAG 2 cut(s) 899, 1010
Sse9I AATT 7 cut(s) 572, 699, 743, 862, 1045, 1243, 1258
SsiI CCGC 1 cut(s) 1238
SspI AATATT 1 cut(s) 954
SspMI CTAG 5 cut(s) 230, 537, 704, 810, 1372
StyD4I CCNGG 3 cut(s) 1140, 1199, 1248
TaiI ACGT 3 cut(s) 333, 1055, 1331
TaqI TCGA 3 cut(s) 486, 1049, 1212
TasI AATT 7 cut(s) 572, 699, 743, 862, 1045, 1243, 1258
TatI WGTACW 3 cut(s) 169, 533, 913
TfiI GAWTC 4 cut(s) 154, 341, 404, 677
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 1 cut(s) 310
Tsp45I GTSAC 1 cut(s) 194
TspDTI ATGAA 4 cut(s) 135, 351, 418, 821
Van91I CCANNNNNTGG 1 cut(s) 1222
Vha464I CTTAAG 1 cut(s) 1010
XapI RAATTY 5 cut(s) 699, 862, 1045, 1243, 1258
XbaI TCTAGA 1 cut(s) 703
XceI RCATGY 2 cut(s) 8, 1358
XcmI CCANNNNNNNNNTGG 2 cut(s) 385, 458
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 5 cut(s) 230, 537, 704, 810, 1372
ZrmI AGTACT 1 cut(s) 535
Zsp2I ATGCAT 3 cut(s) 6, 120, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.