pycom14g10850

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012750
Physical Location & Seq
Forward (+)
13666022 .. 13667445
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 948 bp
ATGGATACTTCTCAGTGGCCACAGGGAATTGTGGTGAACCCAATAGAAGAGATAGTGACAAACTCATGCCCTAAGCCTGCAGCAGCTAATAATTTAGAGAGGAAGGCAAGGCCTCAGAAGGAACAAGCCCTAAACTGTCCAAGGTGCAACTCCACCAACACCAAATTCTGCTACTACAACAACTACAGCCTCACACAACCAAGGTACTTCTGCAAGACTTGCAGAAGGTACTGGACTGAAGGTGGGTCCCTCAGAAACATCCCAGTTGGAGGTGGTTCGAGGAAGAACAAGAGATCTTCATCCTTAAACAATTCTCCATCAACAACTTCTAACAATTCTTCTTCTTCCTCAGCATCCAAAAGGCTTCCTGATCTGATTCACCAAAACCCTAAAATTAATCAAGCCCAAGATCTGAACCTCACTTTCCCATCCACTAATCAAGATTTCAGGAATATTATCTCTGATCACTTGCTTAATCACCATCATCAACAATTTCCAAACAACAACATCAATAACGAAAACACCAACAAGCAGCAAAACCACATTTCTTCTTCTTCCCCAACTTCTACTTCTACAACTACAACAACATCCCACCTTTCAGCCTTGGAGCTTCTCACTGGGTTCACTTCTAGGGGTTTGAACTCCTTCATGCCTATGCCAGTCTCATCAGACCCCAACAACAACAGCAACACTATATATAATAGTCCCTCTGGACTAATTAATCCCATGCAGGATTTCAAGCCAACTCTTAATTTTTCACTTGACGGGCTTGGAAGTGGGTATGGGAGTCTTCAAGGTCATCATGTTCAAGAGAGCAGTGGGAGGCTTTTGTTTCCATTTGAAGACTTGAAACAAGCTCCTTCAGGAGGTGGGATTAATGAGCAGAACAAGGAGCATGGAGATTCAGCTGGGTATTGGACTGGAATGCTAGGAGGAGGATCATGGTAA

Protein Analysis

316

Amino Acids

34.64

Weight (kDa)

8.87

Isoelectric Point (pI)

55.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 42 - 98 1.1e-33 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012750)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24060
fragaria_vesca FvH4_5g11410
malus_domestica MD06G1114000.v1.1 MD14G1135300.v1.1
prunus_persica Prupe.5G126800_v2.0.a1
pyrus_communis pycom06g10660 pycom14g10850
rosa_chinensis RchiOBHm_Chr7g0186471
rosa_laevigata RLG00000004843
rosa_multiflora Rmu_sc0000645.1_g000033
rosa_roxburghii Rroxscaffold_3G00268120
rosa_rugosa Rorug06G0474500
rosa_samantha Rh7AG066800 Rh7BG082600 Rh7CG081100 Rh7DG081800
rosa_wichuraiana Rw7G006800 Rw7G007040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 946
AcoI YGGCCR 1 cut(s) 17
AcsI RAATTY 1 cut(s) 164
AcuI CTGAAG 2 cut(s) 258, 846
AfaI GTAC 2 cut(s) 206, 230
AfiI CCNNNNNNNGG 2 cut(s) 269, 866
AgsI TTSAA 6 cut(s) 640, 739, 794, 809, 842, 850
AjuI GAANNNNNNNTTGG 4 cut(s) 149, 181, 553, 585
AluBI AGCT 4 cut(s) 86, 610, 857, 908
AluI AGCT 4 cut(s) 86, 610, 857, 908
Alw26I GTCTC 1 cut(s) 667
AlwI GGATC 1 cut(s) 946
AoxI GGCC 2 cut(s) 17, 110
ApeKI GCWGC 3 cut(s) 80, 83, 532
ApoI RAATTY 1 cut(s) 164
AseI ATTAAT 3 cut(s) 396, 720, 876
Asp700I GAANNNNTTC 1 cut(s) 644
AspS9I GGNCC 1 cut(s) 246
AsuHPI GGTGA 3 cut(s) 46, 371, 470
AvaII GGWCC 1 cut(s) 246
BalI TGGCCA 1 cut(s) 19
BbsI GAAGAC 2 cut(s) 782, 849
BbvCI CCTCAGC 1 cut(s) 349
BbvI GCAGC 3 cut(s) 92, 95, 544
BccI CCATC 3 cut(s) 325, 436, 489
BclI TGATCA 1 cut(s) 463
BcoDI GTCTC 1 cut(s) 667
BfaI CTAG 2 cut(s) 630, 929
BfmI CTRYAG 2 cut(s) 78, 184
BglII AGATCT 2 cut(s) 293, 409
BisI GCNGC 3 cut(s) 81, 84, 533
BlsI GCNGC 3 cut(s) 82, 85, 534
Bme18I GGWCC 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 246
BmiI GGNNCC 2 cut(s) 247, 248
BmrI ACTGGG 2 cut(s) 257, 627
BmsI GCATC 1 cut(s) 362
BmuI ACTGGG 2 cut(s) 257, 627
BpiI GAAGAC 2 cut(s) 782, 849
Bpu10I CCTNAGC 2 cut(s) 72, 349
BsaBI GATNNNNATC 1 cut(s) 298
BsaJI CCNNGG 3 cut(s) 140, 200, 603
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 866
Bse1I ACTGG 5 cut(s) 236, 263, 622, 659, 925
Bse8I GATNNNNATC 1 cut(s) 298
BseDI CCNNGG 3 cut(s) 140, 200, 603
BseGI GGATG 5 cut(s) 258, 299, 353, 428, 587
BseJI GATNNNNATC 1 cut(s) 298
BseLI CCNNNNNNNGG 2 cut(s) 269, 866
BseMII CTCAG 4 cut(s) 26, 128, 265, 363
BseNI ACTGG 5 cut(s) 236, 263, 622, 659, 925
BseRI GAGGAG 1 cut(s) 948
BseXI GCAGC 3 cut(s) 92, 95, 544
BseYI CCCAGC 1 cut(s) 908
BshFI GGCC 2 cut(s) 19, 112
BslFI GGGAC 2 cut(s) 232, 690
BslI CCNNNNNNNGG 2 cut(s) 269, 866
BsmAI GTCTC 1 cut(s) 667
BsmFI GGGAC 2 cut(s) 232, 690
BsmI GAATGC 1 cut(s) 930
BsnI GGCC 2 cut(s) 19, 112
Bsp143I GATC 5 cut(s) 293, 370, 409, 463, 938
BspANI GGCC 2 cut(s) 19, 112
BspCNI CTCAG 4 cut(s) 25, 127, 264, 362
BspLI GGNNCC 2 cut(s) 247, 248
BspMAI CTGCAG 1 cut(s) 82
BspPI GGATC 1 cut(s) 946
BsrI ACTGG 5 cut(s) 236, 263, 622, 659, 925
BssECI CCNNGG 3 cut(s) 140, 200, 603
BssMI GATC 5 cut(s) 293, 370, 409, 463, 938
BssT1I CCWWGG 3 cut(s) 140, 200, 603
Bst4CI ACNGT 1 cut(s) 137
Bst6I CTCTTC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 219
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 5 cut(s) 12, 72, 114, 251, 349
BstENI CCTNNNNNAGG 1 cut(s) 864
BstF5I GGATG 5 cut(s) 258, 299, 353, 428, 587
BstKTI GATC 5 cut(s) 296, 373, 412, 466, 941
BstMAI GTCTC 1 cut(s) 667
BstMBI GATC 5 cut(s) 293, 370, 409, 463, 938
BstMWI GCNNNNNNNGC 1 cut(s) 219
BstSFI CTRYAG 2 cut(s) 78, 184
BstV1I GCAGC 3 cut(s) 92, 95, 544
BstV2I GAAGAC 2 cut(s) 782, 849
BstX2I RGATCY 2 cut(s) 293, 409
BstYI RGATCY 2 cut(s) 293, 409
BsuRI GGCC 2 cut(s) 19, 112
BtsCI GGATG 5 cut(s) 258, 299, 353, 428, 587
BtsI GCAGTG 1 cut(s) 823
BtsIMutI CAGTG 3 cut(s) 20, 615, 823
Cac8I GCNNGC 1 cut(s) 78
Cfr13I GGNCC 1 cut(s) 246
Csp6I GTAC 2 cut(s) 205, 229
CviAII CATG 6 cut(s) 66, 649, 727, 803, 896, 942
CviQI GTAC 2 cut(s) 205, 229
DdeI CTNAG 5 cut(s) 12, 72, 114, 251, 349
DpnI GATC 5 cut(s) 295, 372, 411, 465, 940
DpnII GATC 5 cut(s) 293, 370, 409, 463, 938
EaeI YGGCCR 1 cut(s) 17
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Eco130I CCWWGG 3 cut(s) 140, 200, 603
Eco147I AGGCCT 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 246
Eco57I CTGAAG 2 cut(s) 258, 846
EcoNI CCTNNNNNAGG 1 cut(s) 864
EcoO109I RGGNCCY 1 cut(s) 246
EcoT14I CCWWGG 3 cut(s) 140, 200, 603
ErhI CCWWGG 3 cut(s) 140, 200, 603
FaeI CATG 6 cut(s) 69, 652, 730, 806, 899, 945
FaqI GGGAC 2 cut(s) 232, 690
FatI CATG 6 cut(s) 65, 648, 726, 802, 895, 941
FbaI TGATCA 1 cut(s) 463
Fnu4HI GCNGC 3 cut(s) 81, 84, 533
FokI GGATG 5 cut(s) 245, 286, 340, 415, 574
Fsp4HI GCNGC 3 cut(s) 81, 84, 533
FspBI CTAG 2 cut(s) 630, 929
GluI GCNGC 3 cut(s) 81, 84, 533
GsaI CCCAGC 1 cut(s) 912
HaeIII GGCC 2 cut(s) 19, 112
Hin1II CATG 6 cut(s) 69, 652, 730, 806, 899, 945
HinfI GANTC 3 cut(s) 376, 787, 902
HphI GGTGA 3 cut(s) 46, 371, 470
Hpy166II GTNNAC 2 cut(s) 37, 624
Hpy188I TCNGA 6 cut(s) 117, 254, 375, 414, 463, 670
Hpy188III TCNNGA 6 cut(s) 368, 440, 448, 711, 809, 864
Hpy8I GTNNAC 2 cut(s) 37, 624
HpyAV CCTTC 6 cut(s) 97, 112, 219, 233, 655, 870
HpyCH4III ACNGT 1 cut(s) 137
HpyCH4V TGCA 5 cut(s) 80, 147, 213, 222, 730
HpyF10VI GCNNNNNNNGC 1 cut(s) 219
HpyF3I CTNAG 5 cut(s) 12, 72, 114, 251, 349
Hsp92II CATG 6 cut(s) 69, 652, 730, 806, 899, 945
KflI GGGWCCC 1 cut(s) 246
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 5 cut(s) 293, 370, 409, 463, 938
LmnI GCTCC 3 cut(s) 607, 862, 892
Lsp1109I GCAGC 3 cut(s) 92, 95, 544
LweI GCATC 1 cut(s) 362
MaeI CTAG 2 cut(s) 630, 929
MaeIII GTNAC 1 cut(s) 55
MalI GATC 5 cut(s) 295, 372, 411, 465, 940
MboI GATC 5 cut(s) 293, 370, 409, 463, 938
MflI RGATCY 2 cut(s) 293, 409
MlsI TGGCCA 1 cut(s) 19
MluCI AATT 9 cut(s) 27, 91, 164, 310, 334, 393, 491, 717, 751
MluNI TGGCCA 1 cut(s) 19
MlyI GAGTC 1 cut(s) 796
MmeI TCCRAC 1 cut(s) 247
Mox20I TGGCCA 1 cut(s) 19
MroXI GAANNNNTTC 1 cut(s) 644
MscI TGGCCA 1 cut(s) 19
MseI TTAA 6 cut(s) 305, 396, 474, 720, 750, 876
MslI CAYNNNNRTG 1 cut(s) 653
Msp20I TGGCCA 1 cut(s) 19
MspA1I CMGCKG 1 cut(s) 908
Mva1269I GAATGC 1 cut(s) 930
MwoI GCNNNNNNNGC 1 cut(s) 219
NdeII GATC 5 cut(s) 293, 370, 409, 463, 938
NlaIII CATG 6 cut(s) 69, 652, 730, 806, 899, 945
NlaIV GGNNCC 2 cut(s) 247, 248
NmuCI GTSAC 1 cut(s) 55
PceI AGGCCT 1 cut(s) 112
PctI GAATGC 1 cut(s) 930
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 2 cut(s) 376, 902
PkrI GCNGC 3 cut(s) 82, 85, 534
PleI GAGTC 1 cut(s) 795
PpsI GAGTC 1 cut(s) 795
PpuMI RGGWCCY 1 cut(s) 246
PshBI ATTAAT 3 cut(s) 396, 720, 876
Psp5II RGGWCCY 1 cut(s) 246
PspFI CCCAGC 1 cut(s) 908
PspN4I GGNNCC 2 cut(s) 247, 248
PspPI GGNCC 1 cut(s) 246
PspPPI RGGWCCY 1 cut(s) 246
PstI CTGCAG 1 cut(s) 82
PsuI RGATCY 2 cut(s) 293, 409
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 2 cut(s) 206, 230
RsaNI GTAC 2 cut(s) 205, 229
RseI CAYNNNNRTG 1 cut(s) 653
SaqAI TTAA 6 cut(s) 305, 396, 474, 720, 750, 876
SatI GCNGC 3 cut(s) 81, 84, 533
Sau3AI GATC 5 cut(s) 293, 370, 409, 463, 938
Sau96I GGNCC 1 cut(s) 246
SchI GAGTC 1 cut(s) 796
SfaNI GCATC 1 cut(s) 362
SfcI CTRYAG 2 cut(s) 78, 184
SinI GGWCC 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 653
Sse9I AATT 9 cut(s) 27, 91, 164, 310, 334, 393, 491, 717, 751
SseBI AGGCCT 1 cut(s) 112
SspI AATATT 1 cut(s) 454
SspMI CTAG 2 cut(s) 630, 929
StuI AGGCCT 1 cut(s) 112
StyI CCWWGG 3 cut(s) 140, 200, 603
TaaI ACNGT 1 cut(s) 137
TaqI TCGA 1 cut(s) 278
TasI AATT 9 cut(s) 27, 91, 164, 310, 334, 393, 491, 717, 751
TfiI GAWTC 2 cut(s) 376, 902
Tru1I TTAA 6 cut(s) 305, 396, 474, 720, 750, 876
Tru9I TTAA 6 cut(s) 305, 396, 474, 720, 750, 876
TscAI CASTG 3 cut(s) 20, 622, 823
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 3 cut(s) 80, 83, 532
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 2 cut(s) 288, 637
TspRI CASTG 3 cut(s) 20, 622, 823
VpaK11BI GGWCC 1 cut(s) 246
VspI ATTAAT 3 cut(s) 396, 720, 876
XagI CCTNNNNNAGG 1 cut(s) 864
XapI RAATTY 1 cut(s) 164
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 2 cut(s) 630, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.