pycom14g14310

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007820
Physical Location & Seq
Reverse (-)
17041474 .. 17044564
3091 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1374 bp
ATGAGCGCTCCCAGTCCCATGGACGTCTCTATGCAAAGCCCCAAAGACGTCGATGTCCCCAACCAAAACGGCGCCGTGGCAATAGACTCAGCTCCTTCTCCCATTCTTCCCCAAGACAAAGTTCTCGTTTCAGTTGAAGTCAGCCTCAAGCCCTCCAGCACAGCTCGGGCTGACGATGTGCGGTCTGCTGTAGAGAGAATGCTTGAAAAGAGGAGTCTGAGCTACACTGACGGACCTGTGCCTGTGCCGCTTGATGATCAGTTTCTAACAGAAAATGTGCAAAGTATCTGTATTTGTGACACAGATGAGTGGGTGCAAAACCATGACATCCTTTTGTTCTGGCAAGTGAAGCCTGTTGTGCATGTTTTTCAGCTTAGCGAAGAGGGAGCATGTGAGGATGTAAGTGGGGATGGACAACCTTCCACCTTCAACGAATGGATTCTTCCTGCAAAGGAATTCGATGGCTTGTGGGAAAGTTTAATTTATGAATCTGGTCTCAAGCAAAGGTTGCTGCGGTATGCGGCTAGTGCATTGCTTTTCACTGAGAAGGGCGTCAATCCTTTCCTTGTGTCATGGAATCGCATTATTCTTTTACACGGACCTCCAGGGACTGGAAAAACTTCTCTATGTAAAGCACTGGCTCAAAAACTATCCATTCGGTTTAACTCCAGATACCCACAATGCCAGTTGATTGAAGTTAATGCACATTCTTTGTTCAGTAAATGGTTCTCTGAGAGCGGCAAGTTGGTTGCAAAGCTTTTCTCAAAAATTCAGGAGATGGTAGAGGAGGAAAGCAATCTGGTATTTGTTTTGATTGATGAAGTCGAAAGCCTTGCTGCTGCTAGAAAAGCTGCTTTATCTGGCTCCGAACCTTCAGATTCTATTCGGGTAGTGAATGCGCTGCTAACTCAGCTGGATAAATTGAAATCAGCACCAAATGTTATAATTCTGACAACATCCAACATAACTGCTGCTATTGATATTGCGTTTGTTGATCGATCTGATATCAAAGCATATGTTGGTCCCCCAACTTTGCAAGCGCGTTATGAAATCCTCAGATCCTGCTTGCATGAACTTATACGAACAGGAATTTTATCACATCTAGAGGATTGTGATAGCATGCTTTCTAACTTTGCTAGCTTGAGAGAGAAACTGAATATGCCTGAGGTACAAGATGCCCAGACACCACTACACCTGTGTAAACAATTGTTCGAAACTGCAGAAGCATGCGAGGGATTGAGTGGGCGATCTTTAAGAAAGCTTCCGTTCCTAGCACATGCGGCTCTTGCAAATCCTTATGGTTGTGACCCTAGCAAGTTCTTGAGTACAATGACAGATACGGCTAGGAGGGAGCGTTCTGAGCTACCTGACTGA

Protein Analysis

458

Amino Acids

50.49

Weight (kDa)

5.07

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRIP13_N PF23563 39 - 128 1.7e-29 TRIP13 N-terminal domain
AAA_16 PF13191 194 - 315 1.1e-07 AAA ATPase domain
AAA_22 PF13401 194 - 287 1.9e-07 AAA domain
AAA PF00004 196 - 341 5.1e-27 ATPase family associated with various cellular activities (AAA)
AAA_lid_TRIP13_C PF23242 344 - 451 2.4e-40 TRIP13-like, AAA+ ATPase lid C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 944
AasI GACNNNNNNGTC 1 cut(s) 53
AatII GACGTC 2 cut(s) 27, 51
AccB1I GGYRCC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 611
AccBSI CCGCTC 1 cut(s) 738
AccII CGCG 1 cut(s) 1042
AciI CCGC 6 cut(s) 181, 248, 514, 521, 738, 1280
AclWI GGATC 1 cut(s) 1053
AcsI RAATTY 3 cut(s) 455, 768, 1089
AcuI CTGAAG 1 cut(s) 858
AcyI GRCGYC 4 cut(s) 24, 48, 72, 552
AfaI GTAC 2 cut(s) 1170, 1327
AfeI AGCGCT 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 611
AgsI TTSAA 5 cut(s) 137, 206, 430, 695, 925
AjnI CCWGG 1 cut(s) 604
AjuI GAANNNNNNNTTGG 2 cut(s) 105, 137
AloI GAACNNNNNNTCC 2 cut(s) 1339, 1371
Alw26I GTCTC 2 cut(s) 31, 500
AlwI GGATC 1 cut(s) 1053
AlwNI CAGNNNCTG 2 cut(s) 611, 1062
Ama87I CYCGRG 1 cut(s) 165
Aor51HI AGCGCT 1 cut(s) 7
ApeKI GCWGC 6 cut(s) 511, 836, 839, 851, 901, 971
ApoI RAATTY 3 cut(s) 455, 768, 1089
Asp700I GAANNNNTTC 2 cut(s) 438, 619
AspLEI GCGC 4 cut(s) 8, 74, 901, 1042
AspS9I GGNCC 3 cut(s) 233, 599, 1022
AsuII TTCGAA 1 cut(s) 1212
AsuNHI GCTAGC 1 cut(s) 1136
AvaI CYCGRG 1 cut(s) 165
AvaII GGWCC 3 cut(s) 233, 599, 1022
AxyI CCTNAGG 1 cut(s) 1164
BanI GGYRCC 1 cut(s) 71
BbvI GCAGC 6 cut(s) 498, 823, 826, 838, 888, 958
BccI CCATC 3 cut(s) 404, 455, 772
BceAI ACGGC 3 cut(s) 59, 85, 1356
BcgI CGANNNNNNTGC 2 cut(s) 815, 849
BciT130I CCWGG 1 cut(s) 606
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 2 cut(s) 31, 500
BfaI CTAG 7 cut(s) 525, 843, 1103, 1137, 1271, 1311, 1344
BfmI CTRYAG 2 cut(s) 189, 1218
BfoI RGCGCY 2 cut(s) 9, 75
BlpI GCTNAGC 1 cut(s) 374
Bme1390I CCNGG 1 cut(s) 606
Bme18I GGWCC 3 cut(s) 233, 599, 1022
BmeT110I CYCGRG 1 cut(s) 165
BmgT120I GGNCC 3 cut(s) 233, 599, 1022
BmiI GGNNCC 3 cut(s) 73, 865, 1024
BmrFI CCNGG 1 cut(s) 606
BmrI ACTGGG 1 cut(s) 6
BmsI GCATC 1 cut(s) 1165
BmtI GCTAGC 1 cut(s) 1140
BmuI ACTGGG 1 cut(s) 6
BpmI CTGGAG 3 cut(s) 139, 588, 652
Bpu1102I GCTNAGC 1 cut(s) 374
Bpu14I TTCGAA 1 cut(s) 1212
BpuEI CTTGAG 4 cut(s) 131, 482, 1162, 1342
Bsa29I ATCGAT 1 cut(s) 997
BsaHI GRCGYC 4 cut(s) 24, 48, 72, 552
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 3 cut(s) 18, 75, 605
BsaXI ACNNNNNCTCC 2 cut(s) 1339, 1369
Bsc4I CCNNNNNNNGG 1 cut(s) 611
Bse1I ACTGG 4 cut(s) 12, 616, 642, 685
Bse21I CCTNAGG 1 cut(s) 1164
Bse3DI GCAATG 1 cut(s) 530
BseBI CCWGG 1 cut(s) 606
BseCI ATCGAT 1 cut(s) 997
BseDI CCNNGG 3 cut(s) 18, 75, 605
BseGI GGATG 4 cut(s) 327, 403, 415, 956
BseLI CCNNNNNNNGG 1 cut(s) 611
BseMI GCAATG 1 cut(s) 530
BseMII CTCAG 8 cut(s) 102, 209, 534, 723, 923, 1069, 1155, 1350
BseNI ACTGG 4 cut(s) 12, 616, 642, 685
BseRI GAGGAG 2 cut(s) 226, 800
BseXI GCAGC 6 cut(s) 498, 823, 826, 838, 888, 958
Bsh1236I CGCG 1 cut(s) 1042
BshNI GGYRCC 1 cut(s) 71
BshVI ATCGAT 1 cut(s) 997
BsiHKCI CYCGRG 1 cut(s) 165
BslFI GGGAC 3 cut(s) 41, 622, 1008
BslI CCNNNNNNNGG 1 cut(s) 611
BsmAI GTCTC 2 cut(s) 31, 500
BsmBI CGTCTC 1 cut(s) 31
BsmFI GGGAC 3 cut(s) 41, 622, 1008
BsmI GAATGC 2 cut(s) 204, 901
Bso31I GGTCTC 1 cut(s) 500
BsoBI CYCGRG 1 cut(s) 165
Bsp119I TTCGAA 1 cut(s) 1212
Bsp143I GATC 5 cut(s) 256, 994, 998, 1058, 1247
Bsp1720I GCTNAGC 1 cut(s) 374
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 6 cut(s) 181, 248, 514, 521, 738, 1280
BspCNI CTCAG 8 cut(s) 101, 210, 535, 724, 922, 1068, 1156, 1351
BspDI ATCGAT 1 cut(s) 997
BspFNI CGCG 1 cut(s) 1042
BspLI GGNNCC 3 cut(s) 73, 865, 1024
BspMAI CTGCAG 1 cut(s) 1222
BspOI GCTAGC 1 cut(s) 1140
BspPI GGATC 1 cut(s) 1053
BspT104I TTCGAA 1 cut(s) 1212
BspT107I GGYRCC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 500
BsrBI CCGCTC 1 cut(s) 738
BsrDI GCAATG 1 cut(s) 530
BsrI ACTGG 4 cut(s) 12, 616, 642, 685
BssECI CCNNGG 3 cut(s) 18, 75, 605
BssMI GATC 5 cut(s) 256, 994, 998, 1058, 1247
BssNI GRCGYC 4 cut(s) 24, 48, 72, 552
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 606
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 4 cut(s) 24, 48, 72, 552
BstAPI GCANNNNNTGC 1 cut(s) 508
BstBI TTCGAA 1 cut(s) 1212
BstC8I GCNNGC 5 cut(s) 1038, 1067, 1121, 1138, 1228
BstDEI CTNAG 9 cut(s) 88, 218, 374, 543, 732, 909, 1055, 1164, 1359
BstDSI CCRYGG 2 cut(s) 18, 75
BstF5I GGATG 4 cut(s) 327, 403, 415, 956
BstFNI CGCG 1 cut(s) 1042
BstH2I RGCGCY 2 cut(s) 9, 75
BstHHI GCGC 4 cut(s) 8, 74, 901, 1042
BstKTI GATC 5 cut(s) 259, 997, 1001, 1061, 1250
BstMAI GTCTC 2 cut(s) 31, 500
BstMBI GATC 5 cut(s) 256, 994, 998, 1058, 1247
BstNI CCWGG 1 cut(s) 606
BstNSI RCATGY 5 cut(s) 365, 393, 1123, 1230, 1280
BstSCI CCNGG 1 cut(s) 604
BstSFI CTRYAG 2 cut(s) 189, 1218
BstUI CGCG 1 cut(s) 1042
BstV1I GCAGC 6 cut(s) 498, 823, 826, 838, 888, 958
BstX2I RGATCY 1 cut(s) 1058
BstXI CCANNNNNNTGG 1 cut(s) 19
BstYI RGATCY 1 cut(s) 1058
Bsu15I ATCGAT 1 cut(s) 997
Bsu36I CCTNAGG 1 cut(s) 1164
BsuTUI ATCGAT 1 cut(s) 997
BtgI CCRYGG 2 cut(s) 18, 75
BtsCI GGATG 4 cut(s) 327, 403, 415, 956
BtsIMutI CAGTG 3 cut(s) 225, 540, 635
Cac8I GCNNGC 5 cut(s) 1038, 1067, 1121, 1138, 1228
CaiI CAGNNNCTG 2 cut(s) 611, 1062
CfoI GCGC 4 cut(s) 8, 74, 901, 1042
Cfr13I GGNCC 3 cut(s) 233, 599, 1022
ClaI ATCGAT 1 cut(s) 997
CseI GACGC 1 cut(s) 541
Csp6I GTAC 2 cut(s) 1169, 1326
CviAII CATG 9 cut(s) 19, 323, 362, 390, 573, 1070, 1120, 1227, 1277
CviQI GTAC 2 cut(s) 1169, 1326
DdeI CTNAG 9 cut(s) 88, 218, 374, 543, 732, 909, 1055, 1164, 1359
DinI GGCGCC 1 cut(s) 73
DpnI GATC 5 cut(s) 258, 996, 1000, 1060, 1249
DpnII GATC 5 cut(s) 256, 994, 998, 1058, 1247
DrdI GACNNNNNNGTC 1 cut(s) 53
DseDI GACNNNNNNGTC 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 18
Eco31I GGTCTC 1 cut(s) 500
Eco32I GATATC 1 cut(s) 1006
Eco47I GGWCC 3 cut(s) 233, 599, 1022
Eco47III AGCGCT 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 858
Eco81I CCTNAGG 1 cut(s) 1164
Eco88I CYCGRG 1 cut(s) 165
EcoRI GAATTC 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 604
EcoRV GATATC 1 cut(s) 1006
EcoT14I CCWWGG 1 cut(s) 18
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 18
Esp3I CGTCTC 1 cut(s) 31
FaeI CATG 9 cut(s) 22, 326, 365, 393, 576, 1073, 1123, 1230, 1280
FaqI GGGAC 3 cut(s) 41, 622, 1008
FatI CATG 9 cut(s) 18, 322, 361, 389, 572, 1069, 1119, 1226, 1276
FauNDI CATATG 1 cut(s) 1015
FbaI TGATCA 1 cut(s) 256
FokI GGATG 4 cut(s) 314, 410, 422, 943
FspBI CTAG 7 cut(s) 525, 843, 1103, 1137, 1271, 1311, 1344
GlaI GCGC 4 cut(s) 7, 73, 900, 1041
GsuI CTGGAG 3 cut(s) 139, 588, 652
HaeII RGCGCY 2 cut(s) 9, 75
HgaI GACGC 1 cut(s) 541
HhaI GCGC 4 cut(s) 8, 74, 901, 1042
Hin1I GRCGYC 4 cut(s) 24, 48, 72, 552
Hin1II CATG 9 cut(s) 22, 326, 365, 393, 576, 1073, 1123, 1230, 1280
Hin6I GCGC 4 cut(s) 6, 72, 899, 1040
HinP1I GCGC 4 cut(s) 6, 72, 899, 1040
HindIII AAGCTT 2 cut(s) 755, 1259
HinfI GANTC 6 cut(s) 86, 214, 439, 488, 577, 878
Hpy166II GTNNAC 1 cut(s) 1202
Hpy188I TCNGA 8 cut(s) 219, 733, 868, 877, 951, 1003, 1058, 1360
Hpy188III TCNNGA 4 cut(s) 669, 773, 1103, 1321
Hpy8I GTNNAC 1 cut(s) 1202
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 5 cut(s) 105, 429, 436, 541, 882
HpyCH4IV ACGT 2 cut(s) 24, 48
HpyF3I CTNAG 9 cut(s) 88, 218, 374, 543, 732, 909, 1055, 1164, 1359
HpySE526I ACGT 2 cut(s) 24, 48
Hsp92I GRCGYC 4 cut(s) 24, 48, 72, 552
Hsp92II CATG 9 cut(s) 22, 326, 365, 393, 576, 1073, 1123, 1230, 1280
HspAI GCGC 4 cut(s) 6, 72, 899, 1040
KasI GGCGCC 1 cut(s) 71
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 5 cut(s) 256, 994, 998, 1058, 1247
LmnI GCTCC 5 cut(s) 13, 97, 386, 869, 1351
Lsp1109I GCAGC 6 cut(s) 498, 823, 826, 838, 888, 958
LweI GCATC 1 cut(s) 1165
MaeI CTAG 7 cut(s) 525, 843, 1103, 1137, 1271, 1311, 1344
MaeII ACGT 2 cut(s) 24, 48
MaeIII GTNAC 2 cut(s) 296, 1304
MalI GATC 5 cut(s) 258, 996, 1000, 1060, 1249
MbiI CCGCTC 1 cut(s) 738
MboI GATC 5 cut(s) 256, 994, 998, 1058, 1247
MboII GAAGA 3 cut(s) 98, 392, 434
MfeI CAATTG 1 cut(s) 1205
MflI RGATCY 1 cut(s) 1058
MluCI AATT 7 cut(s) 455, 480, 768, 920, 945, 1089, 1205
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 2 cut(s) 80, 223
MmeI TCCRAC 1 cut(s) 984
MroXI GAANNNNTTC 2 cut(s) 438, 619
MseI TTAA 4 cut(s) 479, 663, 699, 1253
MspA1I CMGCKG 1 cut(s) 913
MspR9I CCNGG 1 cut(s) 606
MunI CAATTG 1 cut(s) 1205
Mva1269I GAATGC 2 cut(s) 204, 901
MvaI CCWGG 1 cut(s) 606
MvnI CGCG 1 cut(s) 1042
NarI GGCGCC 1 cut(s) 72
NcoI CCATGG 1 cut(s) 18
NdeI CATATG 1 cut(s) 1015
NdeII GATC 5 cut(s) 256, 994, 998, 1058, 1247
NheI GCTAGC 1 cut(s) 1136
NlaIII CATG 9 cut(s) 22, 326, 365, 393, 576, 1073, 1123, 1230, 1280
NlaIV GGNNCC 3 cut(s) 73, 865, 1024
NmuCI GTSAC 2 cut(s) 296, 1304
NspI RCATGY 5 cut(s) 365, 393, 1123, 1230, 1280
NspV TTCGAA 1 cut(s) 1212
PaeI GCATGC 2 cut(s) 1123, 1230
PctI GAATGC 2 cut(s) 204, 901
PdmI GAANNNNTTC 2 cut(s) 438, 619
PfeI GAWTC 4 cut(s) 439, 488, 577, 878
PflMI CCANNNNNTGG 1 cut(s) 611
PleI GAGTC 2 cut(s) 80, 222
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 2 cut(s) 80, 222
PsiI TTATAA 1 cut(s) 944
Psp6I CCWGG 1 cut(s) 604
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 3 cut(s) 73, 865, 1024
PspPI GGNCC 3 cut(s) 233, 599, 1022
PstI CTGCAG 1 cut(s) 1222
PstNI CAGNNNCTG 2 cut(s) 611, 1062
PsuI RGATCY 1 cut(s) 1058
PvuII CAGCTG 1 cut(s) 913
RsaI GTAC 2 cut(s) 1170, 1327
RsaNI GTAC 2 cut(s) 1169, 1326
SaqAI TTAA 4 cut(s) 479, 663, 699, 1253
Sau3AI GATC 5 cut(s) 256, 994, 998, 1058, 1247
Sau96I GGNCC 3 cut(s) 233, 599, 1022
SchI GAGTC 2 cut(s) 80, 223
ScrFI CCNGG 1 cut(s) 606
SfaNI GCATC 1 cut(s) 1165
SfcI CTRYAG 2 cut(s) 189, 1218
SfoI GGCGCC 1 cut(s) 73
SfuI TTCGAA 1 cut(s) 1212
SinI GGWCC 3 cut(s) 233, 599, 1022
SmlI CTYRAG 4 cut(s) 146, 497, 1141, 1321
SmoI CTYRAG 4 cut(s) 146, 497, 1141, 1321
SphI GCATGC 2 cut(s) 1123, 1230
Sse9I AATT 7 cut(s) 455, 480, 768, 920, 945, 1089, 1205
SsiI CCGC 6 cut(s) 181, 248, 514, 521, 738, 1280
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 7 cut(s) 525, 843, 1103, 1137, 1271, 1311, 1344
StyD4I CCNGG 1 cut(s) 604
StyI CCWWGG 1 cut(s) 18
TaiI ACGT 2 cut(s) 27, 51
TaqI TCGA 5 cut(s) 51, 459, 825, 997, 1212
TasI AATT 7 cut(s) 455, 480, 768, 920, 945, 1089, 1205
TatI WGTACW 1 cut(s) 1325
TauI GCSGC 4 cut(s) 250, 524, 741, 1283
TfiI GAWTC 4 cut(s) 439, 488, 577, 878
Tru1I TTAA 4 cut(s) 479, 663, 699, 1253
Tru9I TTAA 4 cut(s) 479, 663, 699, 1253
TscAI CASTG 3 cut(s) 232, 547, 642
TseFI GTSAC 2 cut(s) 296, 1304
TseI GCWGC 6 cut(s) 511, 836, 839, 851, 901, 971
Tsp45I GTSAC 2 cut(s) 296, 1304
TspDTI ATGAA 4 cut(s) 501, 834, 1062, 1086
TspGWI ACGGA 3 cut(s) 246, 612, 1254
TspRI CASTG 3 cut(s) 232, 547, 642
Van91I CCANNNNNTGG 1 cut(s) 611
VpaK11BI GGWCC 3 cut(s) 233, 599, 1022
XapI RAATTY 3 cut(s) 455, 768, 1089
XbaI TCTAGA 1 cut(s) 1102
XceI RCATGY 5 cut(s) 365, 393, 1123, 1230, 1280
XmnI GAANNNNTTC 2 cut(s) 438, 619
XspI CTAG 7 cut(s) 525, 843, 1103, 1137, 1271, 1311, 1344
ZraI GACGTC 2 cut(s) 25, 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.