pycom14g14320

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0018438
Physical Location & Seq
Forward (+)
17051252 .. 17052959
1708 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1665 bp
ATGGCACTAGCAACCCTTCCCATCTCTCCCGCCAAACCCACAACTGTCGTAACTACCATCCCCCAGTTTCCACACAATCCCAAAACCCTAATTCTTCAGCAATGCAGAACCACCAGAGACCTCAACCAAGTCCATGCCCATCTCATCAAAACCTGCCTCCTCCTCAACCCCGCTATCACCGAAAACATCCTGGAATCCGCCGCCATTCTTCTCCCCAACTCCATGGACTATGCTCTCTCCATTTTTCACAATGTCGACGAACCCGATTCGTTGGCCTGCAACATCATGATAAGGAGCCTCATATACCAGCATTCTCCTCTGGGGGCCATTCTTTTGTTCAAGAAAATGCGCGAAAACTCTGCTGAACCCGATGAGTTCACGTTTTCTAGTGTACTGAAAGCTTGTTCAAGACTAAGAGCATTGAGGGAAGGAGAACAGATCCATGCCCACATTGTGAAATGTGGGTTTAAGGTAAATGGGTTCACTGAGAACACTATGATACTTATGTATGCAAGTTGTGGGGAGCTCGATGTTGCCCGCCGGGTGTTTGATGGATTGCCCGAAAGGGCACTAATGGCGTGGAATTCGATGATGGGGGGTTACGTGAGGAGCGAATGTTGTTGGGGAGATATCGTTACTTTGATTAGCGTTTTGACAGCATGTGGGAGACTGGCCAATTTGGAGCTGGGAGAGTGGATTGGTGAGTATATAGAAGCAAATGGGCTGAAGGGGAACATTGCATTAGTCACTTCTCTGGTTGATATGTATGCAAAATGTGGTCAGGTAGATACTGCAAGACGATTATTTGATCGAATGGATCGAAGACATAATGTTGCGTGGAGTGCCATGATTTCAGGTTATAGCCAAGCAGATAGGTGCAGGGAGGCGCTTGGTCTGTTTCATGACATGCAGAAGGCAAATGTGGATCCCAATGAGGTGACCTTGGTCAGTGTGCTTTGGTCCTGTGCTGTCCTTGGAGCTTTGGAAACTGGAAAATGGGTTGAGTTTTATATAAAGAAGAAGAAAATGAAGCTCACAGTTACCCTTGGAACTACACTAATAGATTTCTATGCAAAATGTGGGTGCGTTGATAGTTCAATTGAAGCATTTAACAGGATGCCTGCGGTAAACGTTATCTCTTGGACAGTGCTAATTCAAGGTCTTGCTAGTAATGGACAGGGCATGAGAGCGCTTGAATACTTCCAATTGATGCAGGAAAAGAATATCAAGCCAAATGACGTGACTTTCATTGCCATTCTTTCTGCCTGTGGCCACACAGGTCTGGTTGACGAGGGTCGAAATCTTTTTACCCGCATGAGTAGAGATTTCAGAATTGAACCGAGGATAGAGCACTATGGTTCTATGGTTGATATCTTGGGTCGAGCTGGGTTGATTGAAGAGGCATATCAGTTCATCAAGAATATGCCTATCCAACCAAATGTTGTGGTTTGGAGGACATTGTTGGCTTCATGCAGAGCACAGAAGAATGTCGAAATTGGTGAGGAATCATTGAAACACATAACAACATTCGAGACCCCTCATAGCAGGGAGTACATTCTGCTCTCAAACATTTATGCATCGGTAGGCAGGACTGACGATGCTCTTCGGGTTGGATATCAAATGAGAGAAAAGGGGATAGAGAAGGCTCCTGGGTGTGTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

555

Amino Acids

61.9

Weight (kDa)

7.16

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 88 - 136 1.5e-08 PPR repeat family
PPR PF01535 278 - 307 2.1e-07 PPR repeat
PPR_2 PF13041 278 - 319 3.7e-09 PPR repeat family
PPR_3 PF13812 376 - 425 4e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 377 - 424 2.2e-10 PPR repeat family
E_motif PF20431 496 - 552 7.6e-13 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018438)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06290
malus_domestica MD14G1171500.v1.1
prunus_persica Prupe.5G165600_v2.0.a1
pyrus_communis pycom14g14320
rosa_chinensis RchiOBHm_Chr7g0192571
rosa_laevigata RLG00000004351
rosa_multiflora Rmu_sc0003108.1_g000042
rosa_rugosa Rorug07G0002200
rosa_samantha Rh7BG129100
rosa_wichuraiana Rw7G010890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 161
AccI GTMKAC 1 cut(s) 255
AccII CGCG 1 cut(s) 351
AciI CCGC 7 cut(s) 30, 171, 198, 201, 538, 1124, 1312
AclI AACGTT 1 cut(s) 1131
AclWI GGATC 4 cut(s) 433, 825, 920, 933
AcoI YGGCCR 2 cut(s) 672, 1270
AcsI RAATTY 1 cut(s) 583
AcuI CTGAAG 2 cut(s) 80, 746
AdeI CACNNNGTG 1 cut(s) 454
AfaI GTAC 2 cut(s) 393, 1553
AfeI AGCGCT 1 cut(s) 1191
AgsI TTSAA 9 cut(s) 340, 408, 1098, 1103, 1157, 1196, 1337, 1397, 1513
AjiI CACGTC 1 cut(s) 1240
AjnI CCWGG 2 cut(s) 189, 1648
AluBI AGCT 6 cut(s) 401, 526, 685, 980, 1033, 1385
AluI AGCT 6 cut(s) 401, 526, 685, 980, 1033, 1385
Alw21I GWGCWC 3 cut(s) 528, 1353, 1480
Alw26I GTCTC 3 cut(s) 111, 661, 1526
AlwI GGATC 4 cut(s) 433, 825, 920, 933
Aor51HI AGCGCT 1 cut(s) 1191
AoxI GGCC 4 cut(s) 273, 324, 672, 1270
ApoI RAATTY 1 cut(s) 583
AspLEI GCGC 3 cut(s) 351, 889, 1192
AspS9I GGNCC 2 cut(s) 324, 960
AsuC2I CCSGG 1 cut(s) 542
AsuHPI GGTGA 4 cut(s) 169, 713, 949, 1511
AvaII GGWCC 1 cut(s) 960
BaeGI GKGCMC 1 cut(s) 571
BaeI ACNNNNGTAYC 2 cut(s) 491, 524
BalI TGGCCA 2 cut(s) 674, 1272
BamHI GGATCC 1 cut(s) 925
BanII GRGCYC 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 829
Bbv12I GWGCWC 3 cut(s) 528, 1353, 1480
BccI CCATC 5 cut(s) 29, 65, 147, 545, 586
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 2 cut(s) 191, 1650
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 3 cut(s) 111, 661, 1526
BfaI CTAG 3 cut(s) 8, 387, 1167
BfoI RGCGCY 2 cut(s) 890, 1193
BfuAI ACCTGC 1 cut(s) 161
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 3 cut(s) 191, 542, 1650
Bme18I GGWCC 1 cut(s) 960
BmgBI CACGTC 1 cut(s) 1240
BmgT120I GGNCC 2 cut(s) 324, 960
BmiI GGNNCC 4 cut(s) 296, 325, 927, 1647
BmrFI CCNGG 3 cut(s) 191, 542, 1650
BmrI ACTGGG 1 cut(s) 58
BmsI GCATC 4 cut(s) 1107, 1200, 1586, 1588
BmuI ACTGGG 1 cut(s) 58
BoxI GACNNNNGTC 2 cut(s) 944, 1293
BpiI GAAGAC 1 cut(s) 829
BpuMI CCSGG 1 cut(s) 542
BsaAI YACGTR 1 cut(s) 604
BsaI GGTCTC 2 cut(s) 111, 1526
BsaJI CCNNGG 6 cut(s) 222, 942, 973, 1045, 1340, 1649
BsaXI ACNNNNNCTCC 4 cut(s) 601, 618, 631, 648
Bse1I ACTGG 3 cut(s) 64, 675, 994
Bse3DI GCAATG 3 cut(s) 107, 735, 1248
BseBI CCWGG 2 cut(s) 191, 1650
BseDI CCNNGG 6 cut(s) 222, 942, 973, 1045, 1340, 1649
BseGI GGATG 3 cut(s) 57, 186, 1122
BseMI GCAATG 3 cut(s) 107, 735, 1248
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 3 cut(s) 64, 675, 994
BseRI GAGGAG 4 cut(s) 149, 152, 306, 622
BseSI GKGCMC 1 cut(s) 571
BseYI CCCAGC 2 cut(s) 685, 1385
BsgI GTGCAG 1 cut(s) 898
Bsh1236I CGCG 1 cut(s) 351
BshFI GGCC 4 cut(s) 275, 326, 674, 1272
BsiHKAI GWGCWC 3 cut(s) 528, 1353, 1480
BsiSI CCGG 1 cut(s) 541
BsmAI GTCTC 3 cut(s) 111, 661, 1526
BsmI GAATGC 1 cut(s) 310
BsnI GGCC 4 cut(s) 275, 326, 674, 1272
Bso31I GGTCTC 2 cut(s) 111, 1526
Bsp1286I GDGCHC 4 cut(s) 528, 571, 1353, 1480
Bsp143I GATC 4 cut(s) 438, 808, 817, 925
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 7 cut(s) 30, 171, 198, 201, 538, 1124, 1312
BspANI GGCC 4 cut(s) 275, 326, 674, 1272
BspCNI CTCAG 1 cut(s) 478
BspFNI CGCG 1 cut(s) 351
BspHI TCATGA 2 cut(s) 285, 901
BspLI GGNNCC 4 cut(s) 296, 325, 927, 1647
BspMI ACCTGC 1 cut(s) 161
BspPI GGATC 4 cut(s) 433, 825, 920, 933
BspQI GCTCTTC 1 cut(s) 1608
BspTNI GGTCTC 2 cut(s) 111, 1526
BsrDI GCAATG 3 cut(s) 107, 735, 1248
BsrI ACTGG 3 cut(s) 64, 675, 994
BssECI CCNNGG 6 cut(s) 222, 942, 973, 1045, 1340, 1649
BssMI GATC 4 cut(s) 438, 808, 817, 925
BssT1I CCWWGG 4 cut(s) 222, 942, 973, 1045
Bst2UI CCWGG 2 cut(s) 191, 1650
Bst4CI ACNGT 3 cut(s) 46, 1039, 1147
Bst6I CTCTTC 2 cut(s) 1392, 1608
BstBAI YACGTR 1 cut(s) 604
BstC8I GCNNGC 3 cut(s) 277, 538, 1122
BstDEI CTNAG 2 cut(s) 413, 486
BstDSI CCRYGG 1 cut(s) 222
BstEII GGTNACC 1 cut(s) 937
BstF5I GGATG 3 cut(s) 57, 186, 1122
BstFNI CGCG 1 cut(s) 351
BstH2I RGCGCY 2 cut(s) 890, 1193
BstHHI GCGC 3 cut(s) 351, 889, 1192
BstKTI GATC 4 cut(s) 441, 811, 820, 928
BstMAI GTCTC 3 cut(s) 111, 661, 1526
BstMBI GATC 4 cut(s) 438, 808, 817, 925
BstMWI GCNNNNNNNGC 2 cut(s) 575, 842
BstNI CCWGG 2 cut(s) 191, 1650
BstNSI RCATGY 2 cut(s) 663, 910
BstPAI GACNNNNGTC 2 cut(s) 944, 1293
BstPI GGTNACC 1 cut(s) 937
BstSCI CCNGG 3 cut(s) 189, 540, 1648
BstSLI GKGCMC 1 cut(s) 571
BstUI CGCG 1 cut(s) 351
BstV2I GAAGAC 1 cut(s) 829
BstX2I RGATCY 2 cut(s) 438, 925
BstXI CCANNNNNNTGG 1 cut(s) 223
BstYI RGATCY 2 cut(s) 438, 925
BsuRI GGCC 4 cut(s) 275, 326, 674, 1272
BtgI CCRYGG 1 cut(s) 222
BtrI CACGTC 1 cut(s) 1240
BtsCI GGATG 3 cut(s) 57, 186, 1122
BtsIMutI CAGTG 3 cut(s) 483, 955, 1152
BveI ACCTGC 1 cut(s) 161
Cac8I GCNNGC 3 cut(s) 277, 538, 1122
CciI TCATGA 2 cut(s) 285, 901
CfoI GCGC 3 cut(s) 351, 889, 1192
Cfr13I GGNCC 2 cut(s) 324, 960
Csp6I GTAC 2 cut(s) 392, 1552
CspCI CAANNNNNGTGG 2 cut(s) 1425, 1460
CviQI GTAC 2 cut(s) 392, 1552
DdeI CTNAG 2 cut(s) 413, 486
DpnI GATC 4 cut(s) 440, 810, 819, 927
DpnII GATC 4 cut(s) 438, 808, 817, 925
DraIII CACNNNGTG 1 cut(s) 454
EaeI YGGCCR 2 cut(s) 672, 1270
Eam1104I CTCTTC 2 cut(s) 1392, 1608
EarI CTCTTC 2 cut(s) 1392, 1608
EciI GGCGGA 1 cut(s) 187
Ecl136II GAGCTC 1 cut(s) 526
Eco130I CCWWGG 4 cut(s) 222, 942, 973, 1045
Eco24I GRGCYC 1 cut(s) 528
Eco31I GGTCTC 2 cut(s) 111, 1526
Eco32I GATATC 3 cut(s) 631, 1372, 1616
Eco47I GGWCC 1 cut(s) 960
Eco47III AGCGCT 1 cut(s) 1191
Eco53kI GAGCTC 1 cut(s) 526
Eco57I CTGAAG 2 cut(s) 80, 746
Eco91I GGTNACC 1 cut(s) 937
EcoICRI GAGCTC 1 cut(s) 526
EcoO65I GGTNACC 1 cut(s) 937
EcoRI GAATTC 1 cut(s) 583
EcoRII CCWGG 2 cut(s) 189, 1648
EcoRV GATATC 3 cut(s) 631, 1372, 1616
EcoT14I CCWWGG 4 cut(s) 222, 942, 973, 1045
EcoT22I ATGCAT 1 cut(s) 1579
EcoT38I GRGCYC 1 cut(s) 528
ErhI CCWWGG 4 cut(s) 222, 942, 973, 1045
FauI CCCGC 4 cut(s) 37, 178, 545, 1319
FblI GTMKAC 1 cut(s) 255
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 3 cut(s) 44, 173, 1129
FriOI GRGCYC 1 cut(s) 528
Fsp4HI GCNGC 1 cut(s) 201
FspBI CTAG 3 cut(s) 8, 387, 1167
GlaI GCGC 3 cut(s) 350, 888, 1191
GluI GCNGC 1 cut(s) 201
GsaI CCCAGC 2 cut(s) 689, 1389
HaeII RGCGCY 2 cut(s) 890, 1193
HaeIII GGCC 4 cut(s) 275, 326, 674, 1272
HapII CCGG 1 cut(s) 541
HhaI GCGC 3 cut(s) 351, 889, 1192
Hin6I GCGC 3 cut(s) 349, 887, 1190
HinP1I GCGC 3 cut(s) 349, 887, 1190
HincII GTYRAC 2 cut(s) 256, 1288
HindII GTYRAC 2 cut(s) 256, 1288
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 3 cut(s) 194, 266, 1505
HpaII CCGG 1 cut(s) 541
HphI GGTGA 4 cut(s) 169, 713, 949, 1511
Hpy166II GTNNAC 6 cut(s) 256, 378, 392, 483, 1129, 1288
Hpy188I TCNGA 1 cut(s) 1331
Hpy188III TCNNGA 6 cut(s) 286, 340, 408, 902, 1417, 1531
Hpy8I GTNNAC 6 cut(s) 256, 378, 392, 483, 1129, 1288
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 5 cut(s) 26, 422, 721, 907, 1636
HpyCH4III ACNGT 3 cut(s) 46, 1039, 1147
HpyCH4IV ACGT 4 cut(s) 380, 603, 1131, 1239
HpyF10VI GCNNNNNNNGC 2 cut(s) 575, 842
HpyF3I CTNAG 2 cut(s) 413, 486
HpySE526I ACGT 4 cut(s) 380, 603, 1131, 1239
HspAI GCGC 3 cut(s) 349, 887, 1190
Kzo9I GATC 4 cut(s) 438, 808, 817, 925
LguI GCTCTTC 1 cut(s) 1608
LmnI GCTCC 6 cut(s) 294, 523, 609, 682, 977, 1651
LweI GCATC 4 cut(s) 1107, 1200, 1586, 1588
MaeI CTAG 3 cut(s) 8, 387, 1167
MaeII ACGT 4 cut(s) 380, 603, 1131, 1239
MaeIII GTNAC 7 cut(s) 49, 599, 634, 745, 937, 1039, 1240
MalI GATC 4 cut(s) 440, 810, 819, 927
MboI GATC 4 cut(s) 438, 808, 817, 925
MboII GAAGA 8 cut(s) 86, 200, 834, 1030, 1033, 1409, 1495, 1595
MfeI CAATTG 2 cut(s) 1098, 1205
MflI RGATCY 2 cut(s) 438, 925
MhlI GDGCHC 4 cut(s) 528, 571, 1353, 1480
MlsI TGGCCA 2 cut(s) 674, 1272
MluCI AATT 8 cut(s) 90, 583, 676, 1098, 1152, 1205, 1332, 1494
MluNI TGGCCA 2 cut(s) 674, 1272
MmeI TCCRAC 2 cut(s) 1456, 1591
Mox20I TGGCCA 2 cut(s) 674, 1272
Mph1103I ATGCAT 1 cut(s) 1579
MscI TGGCCA 2 cut(s) 674, 1272
MseI TTAA 2 cut(s) 468, 1110
Msp20I TGGCCA 2 cut(s) 674, 1272
MspI CCGG 1 cut(s) 541
MspR9I CCNGG 3 cut(s) 191, 542, 1650
MunI CAATTG 2 cut(s) 1098, 1205
Mva1269I GAATGC 1 cut(s) 310
MvaI CCWGG 2 cut(s) 191, 1650
MvnI CGCG 1 cut(s) 351
MwoI GCNNNNNNNGC 2 cut(s) 575, 842
NciI CCSGG 1 cut(s) 542
NcoI CCATGG 1 cut(s) 222
NdeII GATC 4 cut(s) 438, 808, 817, 925
NlaIV GGNNCC 4 cut(s) 296, 325, 927, 1647
NmuCI GTSAC 3 cut(s) 745, 937, 1240
NsiI ATGCAT 1 cut(s) 1579
NspI RCATGY 2 cut(s) 663, 910
PagI TCATGA 2 cut(s) 285, 901
PciSI GCTCTTC 1 cut(s) 1608
PcsI WCGNNNNNNNCGW 3 cut(s) 261, 609, 817
PctI GAATGC 1 cut(s) 310
PfeI GAWTC 3 cut(s) 194, 266, 1505
PfoI TCCNGGA 1 cut(s) 189
PkrI GCNGC 1 cut(s) 202
Ppu21I YACGTR 1 cut(s) 604
PshAI GACNNNNGTC 2 cut(s) 944, 1293
Psp124BI GAGCTC 1 cut(s) 528
Psp1406I AACGTT 1 cut(s) 1131
Psp6I CCWGG 2 cut(s) 189, 1648
PspEI GGTNACC 1 cut(s) 937
PspFI CCCAGC 2 cut(s) 685, 1385
PspGI CCWGG 2 cut(s) 189, 1648
PspN4I GGNNCC 4 cut(s) 296, 325, 927, 1647
PspPI GGNCC 2 cut(s) 324, 960
PsuI RGATCY 2 cut(s) 438, 925
RsaI GTAC 2 cut(s) 393, 1553
RsaNI GTAC 2 cut(s) 392, 1552
SacI GAGCTC 1 cut(s) 528
SalI GTCGAC 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 1608
SaqAI TTAA 2 cut(s) 468, 1110
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 4 cut(s) 438, 808, 817, 925
Sau96I GGNCC 2 cut(s) 324, 960
ScrFI CCNGG 3 cut(s) 191, 542, 1650
SduI GDGCHC 4 cut(s) 528, 571, 1353, 1480
SfaNI GCATC 4 cut(s) 1107, 1200, 1586, 1588
SinI GGWCC 1 cut(s) 960
Sse9I AATT 8 cut(s) 90, 583, 676, 1098, 1152, 1205, 1332, 1494
SsiI CCGC 7 cut(s) 30, 171, 198, 201, 538, 1124, 1312
SspMI CTAG 3 cut(s) 8, 387, 1167
SstI GAGCTC 1 cut(s) 528
StyD4I CCNGG 3 cut(s) 189, 540, 1648
StyI CCWWGG 4 cut(s) 222, 942, 973, 1045
TaaI ACNGT 3 cut(s) 46, 1039, 1147
TaiI ACGT 4 cut(s) 383, 606, 1134, 1242
TaqI TCGA 9 cut(s) 255, 528, 587, 811, 820, 1297, 1381, 1491, 1530
TasI AATT 8 cut(s) 90, 583, 676, 1098, 1152, 1205, 1332, 1494
TatI WGTACW 2 cut(s) 391, 1551
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 3 cut(s) 194, 266, 1505
Tru1I TTAA 2 cut(s) 468, 1110
Tru9I TTAA 2 cut(s) 468, 1110
TscAI CASTG 3 cut(s) 490, 955, 1152
TseFI GTSAC 3 cut(s) 745, 937, 1240
Tsp45I GTSAC 3 cut(s) 745, 937, 1240
TspDTI ATGAA 5 cut(s) 890, 1043, 1237, 1402, 1458
TspRI CASTG 3 cut(s) 490, 955, 1152
VpaK11BI GGWCC 1 cut(s) 960
XapI RAATTY 1 cut(s) 583
XceI RCATGY 2 cut(s) 663, 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 682
XmiI GTMKAC 1 cut(s) 255
XspI CTAG 3 cut(s) 8, 387, 1167
Zsp2I ATGCAT 1 cut(s) 1579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.