pycom14g15910

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008596
Physical Location & Seq
Reverse (-)
18366327 .. 18367651
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 657 bp
ATGAGTTCGAAAGAGAAGTACAGAATCCAGCTTCGAACTGCGATTCGACAGCTGAGCGATCGATCCTGCCTCTACGCCGCTTCCAAATGGGCGGCAGAGCAGCTCGTCGGCATCATCGAGCAAGACCCGGTTAAGTTCACGCGTGCGAACACGAGATTCCAGCGCGGGAGCTCCAGCATCCGGCGCAGATTCCGCACCAACGACATCACCCCGACGTCGGCTGCCGGCGTGTCGTATGTGTCCACTCCGGTGATGGAGGAAGATGACATTGTGGACGGCATTTTGACTGCAGGGAGTACAGAAGAGCTGCTCATGTGCTTCGTGATCAGAACGGAAAGAAGTCGTGCTCTTTATCTGGCTGGAGAAAAGCGGAAAGAGGAAGAAATGATGGAACTTGAGGGGCCCCTAGGAAAGAGCGATGTTGTGAATGGTGAACTGGTTTCTCTCGAGAGGGAGTTATCAACCCTTCGCAAGAATCCCATATTGATCCTTCGATGTAACAAAGCTATGGACTCTACTTGTATGGCCTTGTGCTCAAAGACAAAGGCAGCAAGAGTCTTGCTCTTACAGTTCTTGTGGAGTCTGTTAATAGCTACCCTTGGCACTGGAGTGGCTGGTCGGAGCTGCAGTCGTTGTGCACTACAGTTGATGTCTTGA

Protein Analysis

219

Amino Acids

24.31

Weight (kDa)

9.15

Isoelectric Point (pI)

56.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC8 PF04049 7 - 51 1.1e-07 Anaphase promoting complex subunit 8 / Cdc23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 218
AccII CGCG 2 cut(s) 142, 165
AciI CCGC 5 cut(s) 78, 92, 165, 193, 370
AclWI GGATC 2 cut(s) 57, 481
AcyI GRCGYC 1 cut(s) 215
AfaI GTAC 2 cut(s) 20, 298
AfiI CCNNNNNNNGG 2 cut(s) 180, 217
AflIII ACRYGT 1 cut(s) 140
AleI CACNNNNGTG 2 cut(s) 248, 608
AluBI AGCT 8 cut(s) 31, 52, 103, 171, 307, 506, 593, 624
AluI AGCT 8 cut(s) 31, 52, 103, 171, 307, 506, 593, 624
Alw21I GWGCWC 4 cut(s) 173, 349, 536, 640
Alw44I GTGCAC 1 cut(s) 636
AlwI GGATC 2 cut(s) 57, 481
Ama87I CYCGRG 1 cut(s) 446
AoxI GGCC 2 cut(s) 401, 525
ApaI GGGCCC 1 cut(s) 405
ApaLI GTGCAC 1 cut(s) 636
ApeKI GCWGC 5 cut(s) 100, 221, 307, 548, 624
AspA2I CCTAGG 1 cut(s) 406
AspLEI GCGC 2 cut(s) 165, 186
AspS9I GGNCC 2 cut(s) 401, 402
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 3 cut(s) 199, 262, 443
AsuII TTCGAA 2 cut(s) 8, 34
AvaI CYCGRG 1 cut(s) 446
AvrII CCTAGG 1 cut(s) 406
BaeGI GKGCMC 2 cut(s) 405, 640
BanII GRGCYC 2 cut(s) 173, 405
BauI CACGAG 1 cut(s) 151
Bbv12I GWGCWC 4 cut(s) 173, 349, 536, 640
BbvI GCAGC 5 cut(s) 112, 208, 294, 560, 611
BccI CCATC 2 cut(s) 247, 382
BceAI ACGGC 1 cut(s) 292
BclI TGATCA 1 cut(s) 324
BcnI CCSGG 1 cut(s) 128
BfaI CTAG 1 cut(s) 407
BfmI CTRYAG 3 cut(s) 288, 625, 641
BisI GCNGC 7 cut(s) 78, 93, 101, 222, 308, 549, 625
BlnI CCTAGG 1 cut(s) 406
BlpI GCTNAGC 1 cut(s) 53
BlsI GCNGC 7 cut(s) 79, 94, 102, 223, 309, 550, 626
Bme1390I CCNGG 1 cut(s) 128
BmeT110I CYCGRG 1 cut(s) 446
BmgT120I GGNCC 2 cut(s) 401, 402
BmiI GGNNCC 3 cut(s) 402, 403, 404
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 2 cut(s) 120, 186
BplI GAGNNNNNCTC 2 cut(s) 546, 578
BpmI CTGGAG 3 cut(s) 157, 381, 627
Bpu1102I GCTNAGC 1 cut(s) 53
Bpu14I TTCGAA 2 cut(s) 8, 34
BpuEI CTTGAG 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 128
Bsa29I ATCGAT 1 cut(s) 61
BsaHI GRCGYC 1 cut(s) 215
BsaJI CCNNGG 2 cut(s) 406, 598
BsaWI WCCGGW 1 cut(s) 247
BsaXI ACNNNNNCTCC 2 cut(s) 613, 643
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 217
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 2 cut(s) 441, 610
BseCI ATCGAT 1 cut(s) 61
BseDI CCNNGG 2 cut(s) 406, 598
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 180, 217
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 2 cut(s) 441, 610
BseSI GKGCMC 2 cut(s) 405, 640
BseXI GCAGC 5 cut(s) 112, 208, 294, 560, 611
Bsh1236I CGCG 2 cut(s) 142, 165
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 2 cut(s) 403, 527
BshVI ATCGAT 1 cut(s) 61
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 4 cut(s) 173, 349, 536, 640
BsiHKCI CYCGRG 1 cut(s) 446
BsiSI CCGG 4 cut(s) 128, 181, 225, 248
BslI CCNNNNNNNGG 2 cut(s) 180, 217
BsnI GGCC 2 cut(s) 403, 527
BsoBI CYCGRG 1 cut(s) 446
Bsp119I TTCGAA 2 cut(s) 8, 34
Bsp120I GGGCCC 1 cut(s) 401
Bsp1286I GDGCHC 5 cut(s) 173, 349, 405, 536, 640
Bsp143I GATC 4 cut(s) 58, 62, 324, 486
Bsp1720I GCTNAGC 1 cut(s) 53
BspACI CCGC 5 cut(s) 78, 92, 165, 193, 370
BspANI GGCC 2 cut(s) 403, 527
BspCNI CTCAG 1 cut(s) 45
BspDI ATCGAT 1 cut(s) 61
BspFNI CGCG 2 cut(s) 142, 165
BspLI GGNNCC 3 cut(s) 402, 403, 404
BspMAI CTGCAG 2 cut(s) 292, 629
BspPI GGATC 2 cut(s) 57, 481
BspQI GCTCTTC 1 cut(s) 297
BspT104I TTCGAA 2 cut(s) 8, 34
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 2 cut(s) 441, 610
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 2 cut(s) 406, 598
BssMI GATC 4 cut(s) 58, 62, 324, 486
BssNI GRCGYC 1 cut(s) 215
BssSI CACGAG 1 cut(s) 151
BssT1I CCWWGG 2 cut(s) 406, 598
Bst2BI CACGAG 1 cut(s) 151
Bst4CI ACNGT 2 cut(s) 570, 645
Bst6I CTCTTC 1 cut(s) 297
BstACI GRCGYC 1 cut(s) 215
BstBI TTCGAA 2 cut(s) 8, 34
BstC8I GCNNGC 2 cut(s) 144, 226
BstDEI CTNAG 1 cut(s) 53
BstF5I GGATG 1 cut(s) 177
BstFNI CGCG 2 cut(s) 142, 165
BstHHI GCGC 2 cut(s) 165, 186
BstKTI GATC 4 cut(s) 61, 65, 327, 489
BstMBI GATC 4 cut(s) 58, 62, 324, 486
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 2 cut(s) 183, 192
BstSCI CCNGG 1 cut(s) 126
BstSFI CTRYAG 3 cut(s) 288, 625, 641
BstSLI GKGCMC 2 cut(s) 405, 640
BstUI CGCG 2 cut(s) 142, 165
BstV1I GCAGC 5 cut(s) 112, 208, 294, 560, 611
Bsu15I ATCGAT 1 cut(s) 61
BsuRI GGCC 2 cut(s) 403, 527
BsuTUI ATCGAT 1 cut(s) 61
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 603
Cac8I GCNNGC 2 cut(s) 144, 226
CfoI GCGC 2 cut(s) 165, 186
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 2 cut(s) 401, 402
ClaI ATCGAT 1 cut(s) 61
Csp6I GTAC 2 cut(s) 19, 297
CviAII CATG 1 cut(s) 313
CviQI GTAC 2 cut(s) 19, 297
DdeI CTNAG 1 cut(s) 53
DpnI GATC 4 cut(s) 60, 64, 326, 488
DpnII GATC 4 cut(s) 58, 62, 324, 486
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Ecl136II GAGCTC 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 406, 598
Eco24I GRGCYC 2 cut(s) 173, 405
Eco53kI GAGCTC 1 cut(s) 171
Eco88I CYCGRG 1 cut(s) 446
EcoICRI GAGCTC 1 cut(s) 171
EcoO109I RGGNCCY 2 cut(s) 401, 402
EcoT14I CCWWGG 2 cut(s) 406, 598
EcoT38I GRGCYC 2 cut(s) 173, 405
ErhI CCWWGG 2 cut(s) 406, 598
FaeI CATG 1 cut(s) 316
FaiI YATR 5 cut(s) 237, 314, 482, 509, 524
FatI CATG 1 cut(s) 312
FauI CCCGC 1 cut(s) 158
FbaI TGATCA 1 cut(s) 324
Fnu4HI GCNGC 7 cut(s) 78, 93, 101, 222, 308, 549, 625
FokI GGATG 1 cut(s) 164
FriOI GRGCYC 2 cut(s) 173, 405
Fsp4HI GCNGC 7 cut(s) 78, 93, 101, 222, 308, 549, 625
FspBI CTAG 1 cut(s) 407
GlaI GCGC 2 cut(s) 164, 185
GluI GCNGC 7 cut(s) 78, 93, 101, 222, 308, 549, 625
GsuI CTGGAG 3 cut(s) 157, 381, 627
HaeIII GGCC 2 cut(s) 403, 527
HapII CCGG 4 cut(s) 128, 181, 225, 248
HhaI GCGC 2 cut(s) 165, 186
Hin1I GRCGYC 1 cut(s) 215
Hin1II CATG 1 cut(s) 316
Hin6I GCGC 2 cut(s) 163, 184
HinP1I GCGC 2 cut(s) 163, 184
HinfI GANTC 8 cut(s) 24, 43, 156, 189, 475, 512, 555, 580
HpaII CCGG 4 cut(s) 128, 181, 225, 248
HphI GGTGA 3 cut(s) 199, 262, 443
Hpy166II GTNNAC 5 cut(s) 138, 243, 274, 434, 638
Hpy188I TCNGA 2 cut(s) 329, 621
Hpy188III TCNNGA 4 cut(s) 322, 446, 448, 654
Hpy8I GTNNAC 5 cut(s) 138, 243, 274, 434, 638
Hpy99I CGWCG 3 cut(s) 110, 217, 220
HpyAV CCTTC 2 cut(s) 476, 500
HpyCH4III ACNGT 2 cut(s) 570, 645
HpyCH4IV ACGT 1 cut(s) 215
HpyCH4V TGCA 3 cut(s) 290, 627, 638
HpyF10VI GCNNNNNNNGC 2 cut(s) 183, 192
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 215
Hsp92I GRCGYC 1 cut(s) 215
Hsp92II CATG 1 cut(s) 316
HspAI GCGC 2 cut(s) 163, 184
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 324
Kzo9I GATC 4 cut(s) 58, 62, 324, 486
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 3 cut(s) 168, 176, 621
Lsp1109I GCAGC 5 cut(s) 112, 208, 294, 560, 611
LweI GCATC 2 cut(s) 120, 186
MaeI CTAG 1 cut(s) 407
MaeII ACGT 1 cut(s) 215
MaeIII GTNAC 1 cut(s) 497
MalI GATC 4 cut(s) 60, 64, 326, 488
MboI GATC 4 cut(s) 58, 62, 324, 486
MboII GAAGA 3 cut(s) 272, 314, 392
MhlI GDGCHC 5 cut(s) 173, 349, 405, 536, 640
MluI ACGCGT 1 cut(s) 140
MlyI GAGTC 3 cut(s) 506, 564, 589
MmeI TCCRAC 1 cut(s) 599
MnlI CCTC 5 cut(s) 80, 250, 370, 391, 444
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 2 cut(s) 132, 587
MslI CAYNNNNRTG 2 cut(s) 248, 608
MspA1I CMGCKG 1 cut(s) 52
MspI CCGG 4 cut(s) 128, 181, 225, 248
MspR9I CCNGG 1 cut(s) 128
MvnI CGCG 2 cut(s) 142, 165
MwoI GCNNNNNNNGC 2 cut(s) 183, 192
NaeI GCCGGC 1 cut(s) 226
NciI CCSGG 1 cut(s) 128
NdeII GATC 4 cut(s) 58, 62, 324, 486
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 1 cut(s) 316
NlaIV GGNNCC 3 cut(s) 402, 403, 404
NspV TTCGAA 2 cut(s) 8, 34
OliI CACNNNNGTG 2 cut(s) 248, 608
PaeR7I CTCGAG 1 cut(s) 446
PciSI GCTCTTC 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PdiI GCCGGC 1 cut(s) 226
PfeI GAWTC 5 cut(s) 24, 43, 156, 189, 475
PkrI GCNGC 7 cut(s) 79, 94, 102, 223, 309, 550, 626
Ple19I CGATCG 1 cut(s) 61
PleI GAGTC 3 cut(s) 506, 563, 588
PpsI GAGTC 3 cut(s) 506, 563, 588
Psp124BI GAGCTC 1 cut(s) 173
PspN4I GGNNCC 3 cut(s) 402, 403, 404
PspOMI GGGCCC 1 cut(s) 401
PspPI GGNCC 2 cut(s) 401, 402
PstI CTGCAG 2 cut(s) 292, 629
PvuI CGATCG 1 cut(s) 61
PvuII CAGCTG 1 cut(s) 52
RsaI GTAC 2 cut(s) 20, 298
RsaNI GTAC 2 cut(s) 19, 297
RseI CAYNNNNRTG 2 cut(s) 248, 608
SacI GAGCTC 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 2 cut(s) 132, 587
SatI GCNGC 7 cut(s) 78, 93, 101, 222, 308, 549, 625
Sau3AI GATC 4 cut(s) 58, 62, 324, 486
Sau96I GGNCC 2 cut(s) 401, 402
SchI GAGTC 3 cut(s) 506, 564, 589
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 5 cut(s) 173, 349, 405, 536, 640
SetI ASST 9 cut(s) 33, 54, 105, 173, 218, 309, 508, 595, 626
SfaNI GCATC 2 cut(s) 120, 186
SfcI CTRYAG 3 cut(s) 288, 625, 641
Sfr274I CTCGAG 1 cut(s) 446
SfuI TTCGAA 2 cut(s) 8, 34
SlaI CTCGAG 1 cut(s) 446
SmiMI CAYNNNNRTG 2 cut(s) 248, 608
SmlI CTYRAG 2 cut(s) 395, 446
SmoI CTYRAG 2 cut(s) 395, 446
SsiI CCGC 5 cut(s) 78, 92, 165, 193, 370
SspMI CTAG 1 cut(s) 407
SstI GAGCTC 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 126
StyI CCWWGG 2 cut(s) 406, 598
TaaI ACNGT 2 cut(s) 570, 645
TaiI ACGT 1 cut(s) 218
TaqI TCGA 7 cut(s) 8, 34, 46, 61, 117, 447, 493
TatI WGTACW 2 cut(s) 18, 296
TauI GCSGC 2 cut(s) 80, 95
TfiI GAWTC 5 cut(s) 24, 43, 156, 189, 475
Tru1I TTAA 2 cut(s) 132, 587
Tru9I TTAA 2 cut(s) 132, 587
TscAI CASTG 1 cut(s) 610
TseI GCWGC 5 cut(s) 100, 221, 307, 548, 624
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 1 cut(s) 610
VneI GTGCAC 1 cut(s) 636
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XhoI CTCGAG 1 cut(s) 446
XmaJI CCTAGG 1 cut(s) 406
XspI CTAG 1 cut(s) 407
ZraI GACGTC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.