pycom14g19270
TCP Family

T-complex protein 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0016221
Physical Location & Seq
Reverse (-)
20662870 .. 20664672
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1671 bp
ATGGCTTCACCGGCAGTGATCTCCCAGCCGCGCTCCTCCAAGACCGAGTCCTATGTCGACAACAAGCGCAAGGAGGACATCCGCCAGGCCAACATCACCGCCGCTCGAGCCGTCGCCGATGCCGTCCGAACCAGCCTGGGGCCCAAGGGCATGGACAAGATGATCTCCACCGCTAACGGCGAGGTTATCATCACCAACGATGGCGCCACGATTCTTAACAAGATGGAGGTCCTCCAACCCGCCGCCAAGATGCTTGTCGAGCTTTCTAAGTCCCAGGATGCTGCCGCCGGAGATGGAACCACCACCGTCGTCGTCATTGCTGGCGCTCTCCTGAAACAGTGCCAGGTCCTTCTGTCACACGGCATCCACCCGACTGTGATCTCTGACTCTCTCCACAAGGCGTCCACCAAAGCGGTAGACGTCTTGACCGCCATGGCCGTCCCCGTTGAGCTCACAGACCGTGATTCGCTGGTAAAGTCTGCAAGCACATCGCTCAACAGCAAGGTGGTGAGCCAGTACTCGACTCTGCTCGCGCCGATGGCCGTGGACGCCGTGCTCTCCGTGGTGGACCCGGCGAAGCCGGAGATAGTGGACCTTAGGGACATCAGGATCGTGAAGAAGCTCGGAGGAACTGTCGACGATACTGAGATGGTGAAGGGACTTGTTTTCGATAAGAAGGCGAGTCACGCCGCCGGTGGGCCGACGAGGATGGAGAATGCCAAGATTGCTGTGATTCAGTTCCAGATATCGCCTCCGAAAACCGATATCGAGCAGAGCATTGTCGTCTCGGATTATACTCAGATGGATAGGATTCTGAAGGAAGAGAGAAATTACATTCTGGGTATGATAAAAAAGATCAAGGCTACCGGGTGCAACGTTTTGCTGATTCAGAAGAGTATTCTGAGAGATGCAGTTACTGATTTGTCTTTGCATTACTTGGCGAAAGCGAAGATCTTGGTGATCAAAGATGTGGAGAGAGATGAGATTGAGTTCATCACCAAGACTCTGAATTGCTTGCCCATTGCGAATATCGAGCATTTCCGGGCCGAGAAACTGGGGTATGCTGATATGATCACAGGGATTCAGAACATGGGTCGGACCACCACGGTGCTGGTTCGCGGCTCGAATCAACTGGTGCTAGATGAGGCGGAGCGGAGCCTGCACGATGCTCTTTGTGTGGTGAGGTGTTTGGTGAGCAAGAGGTTTTTGATTGCAGGTGGTGGTGCACCAGAGATTGAGCTGTCGAGGCAGTTGGGGGCGTGGGCTAAGGTGCTGCAGGGGATGGAGGGTTATTGTGTGAAGTCGTTTGCTGAGGCGCTCGAGGTTGTTCCCTATACACTGGCTGAGAATGCCGGGTTGAACCCAATTTCGATTGTGACTGAGCTGAGGAATCGCCACGGGCAGATTACCAACATCTTGGAGGAGAATGTGGTGCAGCCATTGCTCGTCAGCACAAGTGCCATCACTCTGGCCACCGAGTGCGTGCGGATGATTTTGAAGATTGATGACATAGTTACTTCTATGTCATTCGAGATGGATTTCTGTTCCGAATTTTGGTACTGGTTCTGGATGTTTACTTTGGATTTGAACTTTGTTTTTGTGTTTGTTAGGCGGATGTTGTGTAACCTACCTTTGCCCATTTATATGCATCTAATTAGTTTGTCGTGTTAA

Protein Analysis

557

Amino Acids

60.97

Weight (kDa)

6.35

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 38 - 507 3.5e-146 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016221)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18190
fragaria_vesca FvH4_7g06380
malus_domestica MD06G1222400.v1.1 MD14G1231500.v1.1
prunus_persica Prupe.5G227000_v2.0.a1
pyrus_communis pycom14g19270
rosa_chinensis RchiOBHm_Chr1g0334481
rosa_laevigata RLG00000029450
rosa_multiflora Rmu_ssc0000404.1_g000012
rosa_roxburghii Rroxscaffold_4G00316810
rosa_rugosa Rorug01G0114200
rosa_samantha Rh1AG139400 Rh1BG105800 Rh1DG143300
rosa_wichuraiana Rw1G011490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1205
AatII GACGTC 1 cut(s) 423
Acc36I ACCTGC 1 cut(s) 1205
AccB1I GGYRCC 1 cut(s) 203
AccBSI CCGCTC 2 cut(s) 104, 1153
AccI GTMKAC 3 cut(s) 57, 417, 636
AccII CGCG 3 cut(s) 31, 533, 1119
AclI AACGTT 1 cut(s) 876
AclWI GGATC 1 cut(s) 617
AcoI YGGCCR 3 cut(s) 435, 540, 1470
AcsI RAATTY 1 cut(s) 1550
AcuI CTGAAG 1 cut(s) 836
AcyI GRCGYC 4 cut(s) 204, 401, 420, 549
AdeI CACNNNGTG 1 cut(s) 1479
AfaI GTAC 2 cut(s) 518, 1559
AfiI CCNNNNNNNGG 3 cut(s) 138, 696, 1554
AgsI TTSAA 3 cut(s) 1360, 1498, 1588
AjnI CCWGG 4 cut(s) 84, 135, 273, 342
AleI CACNNNNGTG 1 cut(s) 1106
AluBI AGCT 5 cut(s) 262, 451, 622, 1240, 1384
AluI AGCT 5 cut(s) 262, 451, 622, 1240, 1384
Alw21I GWGCWC 3 cut(s) 453, 558, 1228
Alw26I GTCTC 1 cut(s) 790
Alw44I GTGCAC 1 cut(s) 1224
AlwI GGATC 1 cut(s) 617
AlwNI CAGNNNCTG 1 cut(s) 917
Ama87I CYCGRG 2 cut(s) 105, 1319
AoxI GGCC 7 cut(s) 87, 140, 435, 540, 698, 1044, 1470
ApaI GGGCCC 1 cut(s) 144
ApaLI GTGCAC 1 cut(s) 1224
ApeKI GCWGC 3 cut(s) 281, 1273, 1435
ApoI RAATTY 1 cut(s) 1550
AspLEI GCGC 6 cut(s) 33, 69, 206, 326, 535, 1318
AspS9I GGNCC 9 cut(s) 140, 141, 229, 346, 568, 592, 698, 1044, 1098
AsuC2I CCSGG 4 cut(s) 572, 868, 1043, 1354
AsuHPI GGTGA 8 cut(s) 88, 184, 520, 664, 970, 988, 1192, 1204
AvaI CYCGRG 2 cut(s) 105, 1319
AvaII GGWCC 5 cut(s) 229, 346, 568, 592, 1098
AxyI CCTNAGG 1 cut(s) 596
BaeGI GKGCMC 2 cut(s) 144, 1228
BalI TGGCCA 1 cut(s) 1472
BanI GGYRCC 1 cut(s) 203
BanII GRGCYC 2 cut(s) 144, 453
Bbv12I GWGCWC 3 cut(s) 453, 558, 1228
BbvCI CCTCAGC 2 cut(s) 1311, 1385
BbvI GCAGC 3 cut(s) 268, 1260, 1447
BceAI ACGGC 7 cut(s) 95, 107, 193, 376, 422, 527, 536
BcgI CGANNNNNNTGC 2 cut(s) 471, 505
BciT130I CCWGG 4 cut(s) 86, 137, 275, 344
BclI TGATCA 2 cut(s) 960, 1071
BcnI CCSGG 4 cut(s) 572, 868, 1043, 1354
BcoDI GTCTC 1 cut(s) 790
BfaI CTAG 1 cut(s) 1139
BfmI CTRYAG 1 cut(s) 1274
BfoI RGCGCY 3 cut(s) 207, 327, 1319
BfuAI ACCTGC 1 cut(s) 1205
BglII AGATCT 1 cut(s) 951
BisI GCNGC 9 cut(s) 29, 102, 243, 282, 285, 690, 1120, 1274, 1436
BlsI GCNGC 9 cut(s) 30, 103, 244, 283, 286, 691, 1121, 1275, 1437
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 8 cut(s) 86, 137, 275, 344, 572, 868, 1043, 1354
Bme18I GGWCC 5 cut(s) 229, 346, 568, 592, 1098
BmeT110I CYCGRG 2 cut(s) 105, 1319
BmgT120I GGNCC 9 cut(s) 140, 141, 229, 346, 568, 592, 698, 1044, 1098
BmiI GGNNCC 6 cut(s) 141, 142, 205, 298, 570, 1157
BmrFI CCNGG 8 cut(s) 86, 137, 275, 344, 572, 868, 1043, 1354
BmrI ACTGGG 1 cut(s) 1064
BmsI GCATC 7 cut(s) 109, 240, 268, 372, 898, 1156, 1657
BmuI ACTGGG 1 cut(s) 1064
Bpu10I CCTNAGC 3 cut(s) 1266, 1311, 1385
BpuMI CCSGG 4 cut(s) 572, 868, 1043, 1354
BsaHI GRCGYC 4 cut(s) 204, 401, 420, 549
BsaJI CCNNGG 8 cut(s) 136, 144, 273, 432, 543, 561, 1104, 1396
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 696, 1554
Bse118I RCCGGY 2 cut(s) 10, 692
Bse1I ACTGG 5 cut(s) 514, 1059, 1137, 1344, 1565
Bse21I CCTNAGG 1 cut(s) 596
Bse3DI GCAATG 3 cut(s) 315, 1020, 1439
BseBI CCWGG 4 cut(s) 86, 137, 275, 344
BseDI CCNNGG 8 cut(s) 136, 144, 273, 432, 543, 561, 1104, 1396
BseGI GGATG 8 cut(s) 78, 283, 363, 714, 1287, 1494, 1575, 1620
BseLI CCNNNNNNNGG 3 cut(s) 138, 696, 1554
BseMI GCAATG 3 cut(s) 315, 1020, 1439
BseMII CTCAG 7 cut(s) 636, 812, 893, 1302, 1335, 1371, 1376
BseNI ACTGG 5 cut(s) 514, 1059, 1137, 1344, 1565
BseRI GAGGAG 2 cut(s) 25, 1436
BseSI GKGCMC 2 cut(s) 144, 1228
BseXI GCAGC 3 cut(s) 268, 1260, 1447
BseYI CCCAGC 1 cut(s) 24
BsgI GTGCAG 2 cut(s) 1145, 1454
Bsh1236I CGCG 3 cut(s) 31, 533, 1119
BshFI GGCC 7 cut(s) 89, 142, 437, 542, 700, 1046, 1472
BshNI GGYRCC 1 cut(s) 203
BsiHKAI GWGCWC 3 cut(s) 453, 558, 1228
BsiHKCI CYCGRG 2 cut(s) 105, 1319
BsiSI CCGG 8 cut(s) 11, 288, 572, 581, 693, 867, 1042, 1353
BslFI GGGAC 4 cut(s) 256, 425, 614, 672
BslI CCNNNNNNNGG 3 cut(s) 138, 696, 1554
BsmAI GTCTC 1 cut(s) 790
BsmBI CGTCTC 1 cut(s) 790
BsmFI GGGAC 4 cut(s) 256, 425, 614, 672
BsmI GAATGC 2 cut(s) 721, 1354
BsnI GGCC 7 cut(s) 89, 142, 437, 542, 700, 1046, 1472
BsoBI CYCGRG 2 cut(s) 105, 1319
Bsp120I GGGCCC 1 cut(s) 140
Bsp1286I GDGCHC 4 cut(s) 144, 453, 558, 1228
Bsp143I GATC 8 cut(s) 18, 162, 378, 609, 855, 951, 960, 1071
Bsp19I CCATGG 1 cut(s) 432
BspANI GGCC 7 cut(s) 89, 142, 437, 542, 700, 1046, 1472
BspCNI CTCAG 7 cut(s) 637, 811, 894, 1303, 1336, 1372, 1377
BspFNI CGCG 3 cut(s) 31, 533, 1119
BspLI GGNNCC 6 cut(s) 141, 142, 205, 298, 570, 1157
BspMAI CTGCAG 1 cut(s) 1278
BspMI ACCTGC 1 cut(s) 1205
BspPI GGATC 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 203
BsrBI CCGCTC 2 cut(s) 104, 1153
BsrDI GCAATG 3 cut(s) 315, 1020, 1439
BsrFI RCCGGY 2 cut(s) 10, 692
BsrI ACTGG 5 cut(s) 514, 1059, 1137, 1344, 1565
BssAI RCCGGY 2 cut(s) 10, 692
BssECI CCNNGG 8 cut(s) 136, 144, 273, 432, 543, 561, 1104, 1396
BssMI GATC 8 cut(s) 18, 162, 378, 609, 855, 951, 960, 1071
BssNI GRCGYC 4 cut(s) 204, 401, 420, 549
BssT1I CCWWGG 2 cut(s) 144, 432
Bst2UI CCWGG 4 cut(s) 86, 137, 275, 344
Bst4CI ACNGT 6 cut(s) 307, 339, 376, 461, 634, 1108
Bst6I CTCTTC 2 cut(s) 816, 887
BstACI GRCGYC 4 cut(s) 204, 401, 420, 549
BstAPI GCANNNNNTGC 1 cut(s) 1441
BstC8I GCNNGC 6 cut(s) 322, 484, 531, 1016, 1160, 1484
BstDSI CCRYGG 5 cut(s) 432, 543, 561, 1104, 1396
BstF5I GGATG 8 cut(s) 78, 283, 363, 714, 1287, 1494, 1575, 1620
BstFNI CGCG 3 cut(s) 31, 533, 1119
BstH2I RGCGCY 3 cut(s) 207, 327, 1319
BstHHI GCGC 6 cut(s) 33, 69, 206, 326, 535, 1318
BstKTI GATC 8 cut(s) 21, 165, 381, 612, 858, 954, 963, 1074
BstMAI GTCTC 1 cut(s) 790
BstMBI GATC 8 cut(s) 18, 162, 378, 609, 855, 951, 960, 1071
BstNI CCWGG 4 cut(s) 86, 137, 275, 344
BstSCI CCNGG 8 cut(s) 84, 135, 273, 342, 570, 866, 1041, 1352
BstSFI CTRYAG 1 cut(s) 1274
BstSLI GKGCMC 2 cut(s) 144, 1228
BstUI CGCG 3 cut(s) 31, 533, 1119
BstV1I GCAGC 3 cut(s) 268, 1260, 1447
BstX2I RGATCY 1 cut(s) 951
BstXI CCANNNNNNTGG 4 cut(s) 151, 1111, 1417, 1468
BstYI RGATCY 1 cut(s) 951
Bsu36I CCTNAGG 1 cut(s) 596
BsuRI GGCC 7 cut(s) 89, 142, 437, 542, 700, 1046, 1472
BtgI CCRYGG 5 cut(s) 432, 543, 561, 1104, 1396
BtgZI GCGATG 1 cut(s) 474
BtsCI GGATG 8 cut(s) 78, 283, 363, 714, 1287, 1494, 1575, 1620
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 3 cut(s) 21, 344, 1337
BveI ACCTGC 1 cut(s) 1205
Cac8I GCNNGC 6 cut(s) 322, 484, 531, 1016, 1160, 1484
CaiI CAGNNNCTG 1 cut(s) 917
CfoI GCGC 6 cut(s) 33, 69, 206, 326, 535, 1318
Cfr10I RCCGGY 2 cut(s) 10, 692
Cfr13I GGNCC 9 cut(s) 140, 141, 229, 346, 568, 592, 698, 1044, 1098
CseI GACGC 2 cut(s) 390, 557
Csp6I GTAC 2 cut(s) 517, 1558
CviAII CATG 3 cut(s) 151, 433, 1090
CviQI GTAC 2 cut(s) 517, 1558
DinI GGCGCC 1 cut(s) 205
DpnI GATC 8 cut(s) 20, 164, 380, 611, 857, 953, 962, 1073
DpnII GATC 8 cut(s) 18, 162, 378, 609, 855, 951, 960, 1071
DraIII CACNNNGTG 1 cut(s) 1479
EaeI YGGCCR 3 cut(s) 435, 540, 1470
Eam1104I CTCTTC 2 cut(s) 816, 887
EarI CTCTTC 2 cut(s) 816, 887
EciI GGCGGA 3 cut(s) 71, 1163, 1627
Ecl136II GAGCTC 1 cut(s) 451
Eco130I CCWWGG 2 cut(s) 144, 432
Eco24I GRGCYC 2 cut(s) 144, 453
Eco32I GATATC 2 cut(s) 747, 766
Eco47I GGWCC 5 cut(s) 229, 346, 568, 592, 1098
Eco53kI GAGCTC 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 836
Eco81I CCTNAGG 1 cut(s) 596
Eco88I CYCGRG 2 cut(s) 105, 1319
EcoICRI GAGCTC 1 cut(s) 451
EcoO109I RGGNCCY 3 cut(s) 140, 229, 346
EcoRII CCWGG 4 cut(s) 84, 135, 273, 342
EcoRV GATATC 2 cut(s) 747, 766
EcoT14I CCWWGG 2 cut(s) 144, 432
EcoT22I ATGCAT 1 cut(s) 1650
EcoT38I GRGCYC 2 cut(s) 144, 453
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
ErhI CCWWGG 2 cut(s) 144, 432
Esp3I CGTCTC 1 cut(s) 790
FaeI CATG 3 cut(s) 154, 436, 1093
FaqI GGGAC 4 cut(s) 256, 425, 614, 672
FatI CATG 3 cut(s) 150, 432, 1089
FauI CCCGC 1 cut(s) 247
FbaI TGATCA 2 cut(s) 960, 1071
FblI GTMKAC 3 cut(s) 57, 417, 636
Fnu4HI GCNGC 9 cut(s) 29, 102, 243, 282, 285, 690, 1120, 1274, 1436
FokI GGATG 8 cut(s) 65, 290, 350, 721, 1294, 1501, 1582, 1627
FriOI GRGCYC 2 cut(s) 144, 453
Fsp4HI GCNGC 9 cut(s) 29, 102, 243, 282, 285, 690, 1120, 1274, 1436
FspBI CTAG 1 cut(s) 1139
GlaI GCGC 6 cut(s) 32, 68, 205, 325, 534, 1317
GluI GCNGC 9 cut(s) 29, 102, 243, 282, 285, 690, 1120, 1274, 1436
GsaI CCCAGC 1 cut(s) 28
HaeII RGCGCY 3 cut(s) 207, 327, 1319
HaeIII GGCC 7 cut(s) 89, 142, 437, 542, 700, 1046, 1472
HapII CCGG 8 cut(s) 11, 288, 572, 581, 693, 867, 1042, 1353
HgaI GACGC 2 cut(s) 390, 557
HhaI GCGC 6 cut(s) 33, 69, 206, 326, 535, 1318
Hin1I GRCGYC 4 cut(s) 204, 401, 420, 549
Hin1II CATG 3 cut(s) 154, 436, 1093
Hin6I GCGC 6 cut(s) 31, 67, 204, 324, 533, 1316
HinP1I GCGC 6 cut(s) 31, 67, 204, 324, 533, 1316
HincII GTYRAC 2 cut(s) 58, 637
HindII GTYRAC 2 cut(s) 58, 637
HpaII CCGG 8 cut(s) 11, 288, 572, 581, 693, 867, 1042, 1353
HphI GGTGA 8 cut(s) 88, 184, 520, 664, 970, 988, 1192, 1204
Hpy166II GTNNAC 9 cut(s) 58, 405, 418, 547, 568, 592, 637, 1226, 1575
Hpy188III TCNNGA 7 cut(s) 331, 424, 607, 613, 742, 1531, 1567
Hpy8I GTNNAC 9 cut(s) 58, 405, 418, 547, 568, 592, 637, 1226, 1575
Hpy99I CGWCG 5 cut(s) 116, 311, 314, 641, 706
HpyAV CCTTC 4 cut(s) 359, 649, 670, 811
HpyCH4III ACNGT 6 cut(s) 307, 339, 376, 461, 634, 1108
HpyCH4IV ACGT 2 cut(s) 420, 876
HpySE526I ACGT 2 cut(s) 420, 876
Hsp92I GRCGYC 4 cut(s) 204, 401, 420, 549
Hsp92II CATG 3 cut(s) 154, 436, 1093
HspAI GCGC 6 cut(s) 31, 67, 204, 324, 533, 1316
KasI GGCGCC 1 cut(s) 203
Ksp22I TGATCA 2 cut(s) 960, 1071
Kzo9I GATC 8 cut(s) 18, 162, 378, 609, 855, 951, 960, 1071
LmnI GCTCC 3 cut(s) 38, 1150, 1155
Lsp1109I GCAGC 3 cut(s) 268, 1260, 1447
LweI GCATC 7 cut(s) 109, 240, 268, 372, 898, 1156, 1657
MaeI CTAG 1 cut(s) 1139
MaeII ACGT 2 cut(s) 420, 876
MaeIII GTNAC 6 cut(s) 354, 683, 913, 1375, 1513, 1622
MalI GATC 8 cut(s) 20, 164, 380, 611, 857, 953, 962, 1073
MbiI CCGCTC 2 cut(s) 104, 1153
MboI GATC 8 cut(s) 18, 162, 378, 609, 855, 951, 960, 1071
MboII GAAGA 5 cut(s) 628, 833, 904, 961, 1510
MflI RGATCY 1 cut(s) 951
MhlI GDGCHC 4 cut(s) 144, 453, 558, 1228
MlsI TGGCCA 1 cut(s) 1472
MluCI AATT 5 cut(s) 829, 1009, 1365, 1550, 1653
MluNI TGGCCA 1 cut(s) 1472
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 5 cut(s) 56, 380, 517, 691, 997
MmeI TCCRAC 2 cut(s) 259, 1076
Mox20I TGGCCA 1 cut(s) 1472
Mph1103I ATGCAT 1 cut(s) 1650
MscI TGGCCA 1 cut(s) 1472
MseI TTAA 2 cut(s) 216, 1669
MslI CAYNNNNRTG 2 cut(s) 1106, 1643
Msp20I TGGCCA 1 cut(s) 1472
MspI CCGG 8 cut(s) 11, 288, 572, 581, 693, 867, 1042, 1353
MspR9I CCNGG 8 cut(s) 86, 137, 275, 344, 572, 868, 1043, 1354
Mva1269I GAATGC 2 cut(s) 721, 1354
MvaI CCWGG 4 cut(s) 86, 137, 275, 344
MvnI CGCG 3 cut(s) 31, 533, 1119
NarI GGCGCC 1 cut(s) 204
NciI CCSGG 4 cut(s) 572, 868, 1043, 1354
NcoI CCATGG 1 cut(s) 432
NdeII GATC 8 cut(s) 18, 162, 378, 609, 855, 951, 960, 1071
NlaIII CATG 3 cut(s) 154, 436, 1093
NlaIV GGNNCC 6 cut(s) 141, 142, 205, 298, 570, 1157
NmeAIII GCCGAG 1 cut(s) 1072
NmuCI GTSAC 3 cut(s) 354, 683, 1375
NsiI ATGCAT 1 cut(s) 1650
OliI CACNNNNGTG 1 cut(s) 1106
PaeR7I CTCGAG 2 cut(s) 105, 1319
PaqCI CACCTGC 1 cut(s) 1205
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 2 cut(s) 721, 1354
PfeI GAWTC 8 cut(s) 211, 464, 733, 811, 886, 1081, 1126, 1390
PflFI GACNNNGTC 1 cut(s) 46
PkrI GCNGC 9 cut(s) 30, 103, 244, 283, 286, 691, 1121, 1275, 1437
PleI GAGTC 5 cut(s) 55, 380, 517, 690, 997
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 5 cut(s) 55, 380, 517, 690, 997
PpuMI RGGWCCY 2 cut(s) 229, 346
Psp124BI GAGCTC 1 cut(s) 453
Psp1406I AACGTT 1 cut(s) 876
Psp5II RGGWCCY 2 cut(s) 229, 346
Psp6I CCWGG 4 cut(s) 84, 135, 273, 342
PspFI CCCAGC 1 cut(s) 24
PspGI CCWGG 4 cut(s) 84, 135, 273, 342
PspN4I GGNNCC 6 cut(s) 141, 142, 205, 298, 570, 1157
PspOMI GGGCCC 1 cut(s) 140
PspPI GGNCC 9 cut(s) 140, 141, 229, 346, 568, 592, 698, 1044, 1098
PspPPI RGGWCCY 2 cut(s) 229, 346
PspXI VCTCGAGB 2 cut(s) 105, 1319
PstI CTGCAG 1 cut(s) 1278
PstNI CAGNNNCTG 1 cut(s) 917
PsuI RGATCY 1 cut(s) 951
PsyI GACNNNGTC 1 cut(s) 46
RsaI GTAC 2 cut(s) 518, 1559
RsaNI GTAC 2 cut(s) 517, 1558
RseI CAYNNNNRTG 2 cut(s) 1106, 1643
SacI GAGCTC 1 cut(s) 453
SalI GTCGAC 2 cut(s) 56, 635
SaqAI TTAA 2 cut(s) 216, 1669
SatI GCNGC 9 cut(s) 29, 102, 243, 282, 285, 690, 1120, 1274, 1436
Sau3AI GATC 8 cut(s) 18, 162, 378, 609, 855, 951, 960, 1071
Sau96I GGNCC 9 cut(s) 140, 141, 229, 346, 568, 592, 698, 1044, 1098
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 5 cut(s) 56, 380, 517, 691, 997
ScrFI CCNGG 8 cut(s) 86, 137, 275, 344, 572, 868, 1043, 1354
SduI GDGCHC 4 cut(s) 144, 453, 558, 1228
SfaNI GCATC 7 cut(s) 109, 240, 268, 372, 898, 1156, 1657
SfcI CTRYAG 1 cut(s) 1274
SfoI GGCGCC 1 cut(s) 205
Sfr274I CTCGAG 2 cut(s) 105, 1319
SgrAI CRCCGGYG 1 cut(s) 692
SinI GGWCC 5 cut(s) 229, 346, 568, 592, 1098
SlaI CTCGAG 2 cut(s) 105, 1319
SmiMI CAYNNNNRTG 2 cut(s) 1106, 1643
SmlI CTYRAG 2 cut(s) 105, 1319
SmoI CTYRAG 2 cut(s) 105, 1319
Sse9I AATT 5 cut(s) 829, 1009, 1365, 1550, 1653
SspDI GGCGCC 1 cut(s) 203
SspMI CTAG 1 cut(s) 1139
SstI GAGCTC 1 cut(s) 453
StyD4I CCNGG 8 cut(s) 84, 135, 273, 342, 570, 866, 1041, 1352
StyI CCWWGG 2 cut(s) 144, 432
TaaI ACNGT 6 cut(s) 307, 339, 376, 461, 634, 1108
TaiI ACGT 2 cut(s) 423, 879
TaqII GACCGA 1 cut(s) 59
TasI AATT 5 cut(s) 829, 1009, 1365, 1550, 1653
TatI WGTACW 1 cut(s) 516
TauI GCSGC 6 cut(s) 31, 104, 245, 287, 692, 1122
TfiI GAWTC 8 cut(s) 211, 464, 733, 811, 886, 1081, 1126, 1390
Tru1I TTAA 2 cut(s) 216, 1669
Tru9I TTAA 2 cut(s) 216, 1669
TscAI CASTG 3 cut(s) 21, 344, 1344
TseFI GTSAC 3 cut(s) 354, 683, 1375
TseI GCWGC 3 cut(s) 281, 1273, 1435
Tsp45I GTSAC 3 cut(s) 354, 683, 1375
TspDTI ATGAA 1 cut(s) 982
TspGWI ACGGA 1 cut(s) 550
TspRI CASTG 3 cut(s) 21, 344, 1344
Tth111I GACNNNGTC 1 cut(s) 46
VneI GTGCAC 1 cut(s) 1224
VpaK11BI GGWCC 5 cut(s) 229, 346, 568, 592, 1098
XapI RAATTY 1 cut(s) 1550
XcmI CCANNNNNNNNNTGG 1 cut(s) 1108
XhoI CTCGAG 2 cut(s) 105, 1319
XmiI GTMKAC 3 cut(s) 57, 417, 636
XspI CTAG 1 cut(s) 1139
ZraI GACGTC 1 cut(s) 421
ZrmI AGTACT 1 cut(s) 518
Zsp2I ATGCAT 1 cut(s) 1650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.