Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0012253

Physical Location & Seq

Genomic Coordinates Reverse (-)

21003472 .. 21008347

Gene Structure 4876 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1779 bp

ATGGCGTCTCAGGCCGCCAATCTGTGGGTTCTGCTGGGTTTAGGTTTGGCTGGAATTCTTCTGATGACAAGGAAGCTGAAGAAAACCATCAGAGAAGACTTTGGCGCCTTTGTTGAGCGGCTTCAACTTCTTCCTCCGCCACAGCCTGCTCCTCCCAAGGCTCCGCACCCCCTCACCGGCCTCACCTTCGCTGTCTCCGACGTATTTGAAATTGAGGGACATGTAACTGGTTTTGGTCATCCTGATTGGGGAAGAACACATGATGCAGCTTCCCGGACGTCGCCTGTGGTTTCCACTCTTGTCGAAGGAGGTGCCACATGCACTGGGATAACTGTTGTGGATGAATTGGCATATAGTATTACTGGAGAAAATAAGCATTATGGTACCCCCACCAATCCAGCAGCATCTTCAAGAAAACCAGGTGGATCCTCTAGTGGAGCTGCTGTGGCTGTAGCCGCTGATCTTGTTGACTTTTCACTGGGTCTTGATACTGTTGGTGGTGTTAGAGTACCTGCTGGGTCTTGTGGCATCATTGGATTCCGAGCTTCACATGGCTCTGTTTCTCATGCTGGATTTATACCTGTTTCTACAAGTCTGGATACTGTTGGGTTTGCTGAAGCCGAGAGGGATCCTAATGTCCTGCGTCGTGTTGGACACGTGCTGCTGCAACTTCCTTATGCAGTTTCACGCAGTCCAAGACAAATTGTAATGGCTGATGATTGCTTTCAAGAAGTAAAGATTCCTGTTGATAGGATTGTTCAAGTGGTTATCAAATCCACTGAGAAGCTTTTTGGAAAACAAGTGTTGAAACATGAAAATATTGGGGACTACTGCAATTCAAAAGTTCCAAGCTTGAAAAAGTTTAATGGCCAGAAGACAAATGGTGAAGTAAAGACTTCATCCCTCAAATTGCTTGCCAATGTTGTGCAGTTTCTTCAAAGGCATGAGTTCAGACATAATCACAGGGAGTGGATTGATTCGGTAAAGCCTGTTTTGGAGTCTGCAACTTCAGCGCAAATATGTGGGCCACTGGAGGCATCCGATGAGGAGATTGAGATATGCAAGTCAATTAGGAATGAATTACAGGCAGCTGTCAGCTCTCTATTGAAGGATGATGGAATTTTGGTTATCCCAACTATAGCAGACCCCCCTGCAAAACTTGGGGGGAAGGAGATGCTCTCTGAGGACTTCCGGAATCGTACTTTTGGTCTCTTAAGTATTGCAAGCCTGTCAGGCTGTTGTCAGGTCACAGCACCTCTGGGATTTTATGACAAGTGCCCTGTTTCAGTTTCTTTCATAGCCAGGCATGGGGGTGATCGCTTTTTACTGGATGCACTGCACACTATGTATACGTCTTTACAGGAGCAGGCCGATATTGCTTCAAAATCCAGATCATCCAAAAATGCTCTCACCAAGGAACAATCTGCTGAAATTGCCAAGGAAAAGGGAAATCAAGCATACAAAGATAAAGAGTGGCAGAAGGCTATTGGCTTTTATTCAGAAGCTATCAAACTTAGTGGCAACAATGCAACGTATTACAGTAACAGGGCTCAAGCATATCTTGAAGTTGGAAGTTTCATTCAAGCGGAGGCAGATTGTACAAAAGCCATCAACCTTGACAAAAAGAATGTGAAAGCTTATTTCCGAAGAGGCACTGCAAGGGAGATGCTTGGTTACTATAAGGAAGCGATTGAAGACTTTAGACACGCACTTGTTCTTGAGCCAACCAACAAACGAGCAGCCGCTGCTGCTGAAAGATTGAGGAAGGTATTCCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0016020 GO:0016021 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120

Pfam Domains

Protein Families

Amidase TPR_11 TPR_16 TPR_2 TPR_8

Protein Analysis

593

Amino Acids

64.21

Weight (kDa)

8.62

Isoelectric Point (pI)

37.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	55 - 204	4.1e-35	Amidase
TPR_BSK1_C	PF25575	443 - 542	2.8e-06	Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_2	PF07719	545 - 577	3.5e-06	Tetratricopeptide repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012253)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17970 AT3G17970
fragaria_vesca	FvH4_5g17380
malus_domestica	MD06G1227100.v1.1 MD14G1236500.v1.1
prunus_persica	Prupe.5G233600_v2.0.a1
pyrus_communis	pycom06g20340 pycom14g19750
rosa_chinensis	RchiOBHm_Chr7g0178361
rosa_laevigata	RLG00000005452
rosa_roxburghii	Rroxscaffold_3G00274980
rosa_rugosa	Rorug06G0416300 Rorug06G0416400
rosa_samantha	Rh7AG015100 Rh7BG014600 Rh7CG014600 Rh7DG014900
rosa_wichuraiana	Rw0G010200 Rw7G001200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	281
Acc36I	ACCTGC	1 cut(s)	520
Acc65I	GGTACC	1 cut(s)	383
AccB1I	GGYRCC	3 cut(s)	104, 311, 383
AccB7I	CCANNNNNTGG	1 cut(s)	24
AccBSI	CCGCTC	1 cut(s)	118
AccI	GTMKAC	1 cut(s)	1349
AccIII	TCCGGA	1 cut(s)	1191
AciI	CCGC	7 cut(s)	15, 118, 137, 164, 456, 1586, 1743
AclWI	GGATC	4 cut(s)	420, 433, 623, 636
AcoI	YGGCCR	1 cut(s)	868
AcsI	RAATTY	2 cut(s)	54, 1119
AcuI	CTGAAG	3 cut(s)	98, 636, 993
AcvI	CACGTG	1 cut(s)	658
AcyI	GRCGYC	3 cut(s)	5, 105, 278
AfaI	GTAC	4 cut(s)	385, 510, 1201, 1600
AfiI	CCNNNNNNNGG	4 cut(s)	24, 176, 248, 1308
AflII	CTTAAG	1 cut(s)	1213
AflIII	ACRYGT	2 cut(s)	220, 655
AjnI	CCWGG	2 cut(s)	418, 1301
Alw26I	GTCTC	3 cut(s)	12, 199, 1214
AlwI	GGATC	4 cut(s)	420, 433, 623, 636
AlwNI	CAGNNNCTG	1 cut(s)	1745
Aor13HI	TCCGGA	1 cut(s)	1191
AoxI	GGCC	5 cut(s)	12, 178, 868, 1025, 1368
ApeKI	GCWGC	9 cut(s)	266, 401, 440, 661, 664, 1088, 1739, 1745, 1748
ApoI	RAATTY	2 cut(s)	54, 1119
Asp700I	GAANNNNTTC	1 cut(s)	1769
Asp718I	GGTACC	1 cut(s)	383
AspLEI	GCGC	2 cut(s)	107, 1015
AspS9I	GGNCC	1 cut(s)	1025
AsuC2I	CCSGG	1 cut(s)	274
AsuHPI	GGTGA	5 cut(s)	166, 175, 896, 1325, 1402
BaeGI	GKGCMC	1 cut(s)	1280
BalI	TGGCCA	1 cut(s)	870
BamHI	GGATCC	2 cut(s)	425, 628
BanI	GGYRCC	3 cut(s)	104, 311, 383
BanII	GRGCYC	1 cut(s)	1552
BbrPI	CACGTG	1 cut(s)	658
BbsI	GAAGAC	3 cut(s)	102, 881, 1701
BbvI	GCAGC	9 cut(s)	278, 413, 427, 648, 651, 1100, 1732, 1735, 1751
BccI	CCATC	3 cut(s)	95, 1109, 1616
BcgI	CGANNNNNNTGC	2 cut(s)	293, 327
BciT130I	CCWGG	2 cut(s)	420, 1303
BciVI	GTATCC	1 cut(s)	592
BcnI	CCSGG	1 cut(s)	274
BcoDI	GTCTC	3 cut(s)	12, 199, 1214
BfaI	CTAG	1 cut(s)	432
BfmI	CTRYAG	3 cut(s)	450, 1137, 1775
BfoI	RGCGCY	1 cut(s)	108
BfrI	CTTAAG	1 cut(s)	1213
BfuAI	ACCTGC	1 cut(s)	520
BfuI	GTATCC	1 cut(s)	592
BglI	GCCNNNNNGGC	1 cut(s)	1233
Bme1390I	CCNGG	3 cut(s)	274, 420, 1303
BmgT120I	GGNCC	1 cut(s)	1025
BmiI	GGNNCC	6 cut(s)	106, 162, 313, 385, 427, 630
BmrFI	CCNGG	3 cut(s)	274, 420, 1303
BmrI	ACTGGG	2 cut(s)	333, 488
BmsI	GCATC	7 cut(s)	253, 413, 537, 1046, 1164, 1321, 1656
BmuI	ACTGGG	2 cut(s)	333, 488
BpiI	GAAGAC	3 cut(s)	102, 881, 1701
BplI	GAGNNNNNCTC	2 cut(s)	1163, 1195
BpmI	CTGGAG	2 cut(s)	384, 1052
BpuEI	CTTGAG	2 cut(s)	1536, 1739
BpuMI	CCSGG	1 cut(s)	274
BsaAI	YACGTR	1 cut(s)	658
BsaHI	GRCGYC	3 cut(s)	5, 105, 278
BsaI	GGTCTC	1 cut(s)	1214
BsaJI	CCNNGG	3 cut(s)	156, 1413, 1437
BsaWI	WCCGGW	1 cut(s)	1191
BsaXI	ACNNNNNCTCC	2 cut(s)	429, 459
Bsc4I	CCNNNNNNNGG	4 cut(s)	24, 176, 248, 1308
Bse118I	RCCGGY	1 cut(s)	176
Bse1I	ACTGG	6 cut(s)	232, 328, 367, 483, 1035, 1332
BseAI	TCCGGA	1 cut(s)	1191
BseBI	CCWGG	2 cut(s)	420, 1303
BseDI	CCNNGG	3 cut(s)	156, 1413, 1437
BseGI	GGATG	7 cut(s)	238, 346, 899, 1037, 1117, 1336, 1394
BseLI	CCNNNNNNNGG	4 cut(s)	24, 176, 248, 1308
BseMII	CTCAG	3 cut(s)	23, 771, 1173
BseNI	ACTGG	6 cut(s)	232, 328, 367, 483, 1035, 1332
BseRI	GAGGAG	2 cut(s)	141, 1061
BseSI	GKGCMC	1 cut(s)	1280
BseXI	GCAGC	9 cut(s)	278, 413, 427, 648, 651, 1100, 1732, 1735, 1751
BseYI	CCCAGC	2 cut(s)	34, 515
BsgI	GTGCAG	2 cut(s)	947, 1322
BshFI	GGCC	5 cut(s)	14, 180, 870, 1027, 1370
BshNI	GGYRCC	3 cut(s)	104, 311, 383
BsiSI	CCGG	3 cut(s)	177, 274, 1192
BslFI	GGGAC	2 cut(s)	231, 839
BslI	CCNNNNNNNGG	4 cut(s)	24, 176, 248, 1308
BsmAI	GTCTC	3 cut(s)	12, 199, 1214
BsmBI	CGTCTC	1 cut(s)	12
BsmFI	GGGAC	2 cut(s)	231, 839
BsnI	GGCC	5 cut(s)	14, 180, 870, 1027, 1370
Bso31I	GGTCTC	1 cut(s)	1214
Bsp1286I	GDGCHC	2 cut(s)	1280, 1552
Bsp13I	TCCGGA	1 cut(s)	1191
Bsp1407I	TGTACA	1 cut(s)	1598
Bsp143I	GATC	5 cut(s)	425, 460, 628, 1315, 1391
BspACI	CCGC	7 cut(s)	15, 118, 137, 164, 456, 1586, 1743
BspANI	GGCC	5 cut(s)	14, 180, 870, 1027, 1370
BspCNI	CTCAG	3 cut(s)	22, 772, 1174
BspEI	TCCGGA	1 cut(s)	1191
BspLI	GGNNCC	6 cut(s)	106, 162, 313, 385, 427, 630
BspMI	ACCTGC	1 cut(s)	520
BspPI	GGATC	4 cut(s)	420, 433, 623, 636
BspT107I	GGYRCC	3 cut(s)	104, 311, 383
BspTI	CTTAAG	1 cut(s)	1213
BspTNI	GGTCTC	1 cut(s)	1214
BsrBI	CCGCTC	1 cut(s)	118
BsrFI	RCCGGY	1 cut(s)	176
BsrGI	TGTACA	1 cut(s)	1598
BsrI	ACTGG	6 cut(s)	232, 328, 367, 483, 1035, 1332
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	3 cut(s)	156, 1413, 1437
BssMI	GATC	5 cut(s)	425, 460, 628, 1315, 1391
BssNAI	GTATAC	1 cut(s)	1350
BssNI	GRCGYC	3 cut(s)	5, 105, 278
BssT1I	CCWWGG	3 cut(s)	156, 1413, 1437
Bst1107I	GTATAC	1 cut(s)	1350
Bst2UI	CCWGG	2 cut(s)	420, 1303
Bst4CI	ACNGT	4 cut(s)	334, 493, 604, 1541
Bst6I	CTCTTC	1 cut(s)	1642
BstACI	GRCGYC	3 cut(s)	5, 105, 278
BstAFI	CTTAAG	1 cut(s)	1213
BstAPI	GCANNNNNTGC	1 cut(s)	1745
BstAUI	TGTACA	1 cut(s)	1598
BstBAI	YACGTR	1 cut(s)	658
BstC8I	GCNNGC	4 cut(s)	147, 915, 1225, 1368
BstDEI	CTNAG	4 cut(s)	9, 780, 1182, 1514
BstF5I	GGATG	7 cut(s)	238, 346, 899, 1037, 1117, 1336, 1394
BstH2I	RGCGCY	1 cut(s)	108
BstHHI	GCGC	2 cut(s)	107, 1015
BstKTI	GATC	5 cut(s)	428, 463, 631, 1318, 1394
BstMAI	GTCTC	3 cut(s)	12, 199, 1214
BstMBI	GATC	5 cut(s)	425, 460, 628, 1315, 1391
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 446, 455, 1010, 1233, 1376, 1433, 1745, 1748
BstNI	CCWGG	2 cut(s)	420, 1303
BstNSI	RCATGY	2 cut(s)	224, 321
BstSCI	CCNGG	3 cut(s)	272, 418, 1301
BstSFI	CTRYAG	3 cut(s)	450, 1137, 1775
BstSLI	GKGCMC	1 cut(s)	1280
BstV1I	GCAGC	9 cut(s)	278, 413, 427, 648, 651, 1100, 1732, 1735, 1751
BstV2I	GAAGAC	3 cut(s)	102, 881, 1701
BstX2I	RGATCY	2 cut(s)	425, 628
BstYI	RGATCY	2 cut(s)	425, 628
BstZ17I	GTATAC	1 cut(s)	1350
BsuI	GTATCC	1 cut(s)	592
BsuRI	GGCC	5 cut(s)	14, 180, 870, 1027, 1370
BtsCI	GGATG	7 cut(s)	238, 346, 899, 1037, 1117, 1336, 1394
BtsI	GCAGTG	2 cut(s)	1334, 1653
BtsIMutI	CAGTG	6 cut(s)	321, 476, 777, 1028, 1334, 1653
BveI	ACCTGC	1 cut(s)	520
Cac8I	GCNNGC	4 cut(s)	147, 915, 1225, 1368
CaiI	CAGNNNCTG	1 cut(s)	1745
CfoI	GCGC	2 cut(s)	107, 1015
Cfr10I	RCCGGY	1 cut(s)	176
Cfr13I	GGNCC	1 cut(s)	1025
CseI	GACGC	1 cut(s)	632
CsiI	ACCWGGT	1 cut(s)	418
Csp6I	GTAC	4 cut(s)	384, 509, 1200, 1599
CspCI	CAANNNNNGTGG	2 cut(s)	1498, 1533
CviAII	CATG	8 cut(s)	221, 260, 318, 551, 566, 812, 944, 1307
CviQI	GTAC	4 cut(s)	384, 509, 1200, 1599
DdeI	CTNAG	4 cut(s)	9, 780, 1182, 1514
DinI	GGCGCC	1 cut(s)	106
DpnI	GATC	5 cut(s)	427, 462, 630, 1317, 1393
DpnII	GATC	5 cut(s)	425, 460, 628, 1315, 1391
EaeI	YGGCCR	1 cut(s)	868
Eam1104I	CTCTTC	1 cut(s)	1642
EarI	CTCTTC	1 cut(s)	1642
EciI	GGCGGA	1 cut(s)	126
Eco130I	CCWWGG	3 cut(s)	156, 1413, 1437
Eco24I	GRGCYC	1 cut(s)	1552
Eco31I	GGTCTC	1 cut(s)	1214
Eco57I	CTGAAG	3 cut(s)	98, 636, 993
Eco72I	CACGTG	1 cut(s)	658
EcoRI	GAATTC	1 cut(s)	54
EcoRII	CCWGG	2 cut(s)	418, 1301
EcoT14I	CCWWGG	3 cut(s)	156, 1413, 1437
EcoT38I	GRGCYC	1 cut(s)	1552
EgeI	GGCGCC	1 cut(s)	106
EheI	GGCGCC	1 cut(s)	106
ErhI	CCWWGG	3 cut(s)	156, 1413, 1437
Esp3I	CGTCTC	1 cut(s)	12
FaeI	CATG	8 cut(s)	224, 263, 321, 554, 569, 815, 947, 1310
FalI	AAGNNNNNCTT	2 cut(s)	1545, 1577
FaqI	GGGAC	2 cut(s)	231, 839
FatI	CATG	8 cut(s)	220, 259, 317, 550, 565, 811, 943, 1306
FblI	GTMKAC	1 cut(s)	1349
FokI	GGATG	7 cut(s)	225, 353, 886, 1024, 1124, 1343, 1381
FriOI	GRGCYC	1 cut(s)	1552
FspBI	CTAG	1 cut(s)	432
GlaI	GCGC	2 cut(s)	106, 1014
GsaI	CCCAGC	2 cut(s)	38, 519
GsuI	CTGGAG	2 cut(s)	384, 1052
HaeII	RGCGCY	1 cut(s)	108
HaeIII	GGCC	5 cut(s)	14, 180, 870, 1027, 1370
HapII	CCGG	3 cut(s)	177, 274, 1192
HgaI	GACGC	1 cut(s)	632
HhaI	GCGC	2 cut(s)	107, 1015
Hin1I	GRCGYC	3 cut(s)	5, 105, 278
Hin1II	CATG	8 cut(s)	224, 263, 321, 554, 569, 815, 947, 1310
Hin6I	GCGC	2 cut(s)	105, 1013
HinP1I	GCGC	2 cut(s)	105, 1013
HincII	GTYRAC	1 cut(s)	469
HindII	GTYRAC	1 cut(s)	469
HindIII	AAGCTT	3 cut(s)	785, 850, 1635
HinfI	GANTC	5 cut(s)	537, 739, 977, 998, 1195
HpaII	CCGG	3 cut(s)	177, 274, 1192
HphI	GGTGA	5 cut(s)	166, 175, 896, 1325, 1402
Hpy166II	GTNNAC	2 cut(s)	469, 1350
Hpy188I	TCNGA	9 cut(s)	63, 92, 199, 542, 953, 1042, 1183, 1501, 1646
Hpy188III	TCNNGA	9 cut(s)	242, 411, 485, 596, 728, 1192, 1389, 1562, 1718
Hpy8I	GTNNAC	2 cut(s)	469, 1350
Hpy99I	CGWCG	3 cut(s)	203, 283, 648
HpyAV	CCTTC	6 cut(s)	196, 299, 1102, 1162, 1474, 1759
HpyCH4III	ACNGT	4 cut(s)	334, 493, 604, 1541
HpyCH4IV	ACGT	5 cut(s)	201, 278, 657, 1352, 1532
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 446, 455, 1010, 1233, 1376, 1433, 1745, 1748
HpyF3I	CTNAG	4 cut(s)	9, 780, 1182, 1514
HpySE526I	ACGT	5 cut(s)	201, 278, 657, 1352, 1532
Hsp92I	GRCGYC	3 cut(s)	5, 105, 278
Hsp92II	CATG	8 cut(s)	224, 263, 321, 554, 569, 815, 947, 1310
HspAI	GCGC	2 cut(s)	105, 1013
KasI	GGCGCC	1 cut(s)	104
Kpn2I	TCCGGA	1 cut(s)	1191
KpnI	GGTACC	1 cut(s)	387
Kzo9I	GATC	5 cut(s)	425, 460, 628, 1315, 1391
LmnI	GCTCC	4 cut(s)	154, 166, 437, 1363
Lsp1109I	GCAGC	9 cut(s)	278, 413, 427, 648, 651, 1100, 1732, 1735, 1751
LweI	GCATC	7 cut(s)	253, 413, 537, 1046, 1164, 1321, 1656
MabI	ACCWGGT	1 cut(s)	418
MaeI	CTAG	1 cut(s)	432
MaeII	ACGT	5 cut(s)	201, 278, 657, 1352, 1532
MaeIII	GTNAC	4 cut(s)	223, 1246, 1541, 1673
MalI	GATC	5 cut(s)	427, 462, 630, 1317, 1393
MbiI	CCGCTC	1 cut(s)	118
MboI	GATC	5 cut(s)	425, 460, 628, 1315, 1391
MflI	RGATCY	2 cut(s)	425, 628
MhlI	GDGCHC	2 cut(s)	1280, 1552
MlsI	TGGCCA	1 cut(s)	870
MluNI	TGGCCA	1 cut(s)	870
Mly113I	GGCGCC	1 cut(s)	105
MlyI	GAGTC	1 cut(s)	1007
MmeI	TCCRAC	3 cut(s)	222, 631, 1549
Mox20I	TGGCCA	1 cut(s)	870
MroI	TCCGGA	1 cut(s)	1191
MroXI	GAANNNNTTC	1 cut(s)	1769
MscI	TGGCCA	1 cut(s)	870
MseI	TTAA	2 cut(s)	864, 1214
MslI	CAYNNNNRTG	1 cut(s)	1311
Msp20I	TGGCCA	1 cut(s)	870
MspA1I	CMGCKG	3 cut(s)	458, 1091, 1745
MspCI	CTTAAG	1 cut(s)	1213
MspI	CCGG	3 cut(s)	177, 274, 1192
MspR9I	CCNGG	3 cut(s)	274, 420, 1303
MvaI	CCWGG	2 cut(s)	420, 1303
MwoI	GCNNNNNNNGC	9 cut(s)	11, 446, 455, 1010, 1233, 1376, 1433, 1745, 1748
NarI	GGCGCC	1 cut(s)	105
NciI	CCSGG	1 cut(s)	274
NdeII	GATC	5 cut(s)	425, 460, 628, 1315, 1391
NlaIII	CATG	8 cut(s)	224, 263, 321, 554, 569, 815, 947, 1310
NlaIV	GGNNCC	6 cut(s)	106, 162, 313, 385, 427, 630
NmeAIII	GCCGAG	1 cut(s)	646
NmuCI	GTSAC	1 cut(s)	1246
NspI	RCATGY	2 cut(s)	224, 321
PciI	ACATGT	1 cut(s)	220
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PdmI	GAANNNNTTC	1 cut(s)	1769
PfeI	GAWTC	4 cut(s)	537, 739, 977, 1195
PflMI	CCANNNNNTGG	1 cut(s)	24
PfoI	TCCNGGA	1 cut(s)	272
PleI	GAGTC	1 cut(s)	1006
PluTI	GGCGCC	1 cut(s)	108
PmaCI	CACGTG	1 cut(s)	658
PmlI	CACGTG	1 cut(s)	658
PpsI	GAGTC	1 cut(s)	1006
Ppu21I	YACGTR	1 cut(s)	658
PscI	ACATGT	1 cut(s)	220
Psp6I	CCWGG	2 cut(s)	418, 1301
PspCI	CACGTG	1 cut(s)	658
PspFI	CCCAGC	2 cut(s)	34, 515
PspGI	CCWGG	2 cut(s)	418, 1301
PspN4I	GGNNCC	6 cut(s)	106, 162, 313, 385, 427, 630
PspPI	GGNCC	1 cut(s)	1025
PstNI	CAGNNNCTG	1 cut(s)	1745
PsuI	RGATCY	2 cut(s)	425, 628
PvuII	CAGCTG	1 cut(s)	1091
RsaI	GTAC	4 cut(s)	385, 510, 1201, 1600
RsaNI	GTAC	4 cut(s)	384, 509, 1200, 1599
RseI	CAYNNNNRTG	1 cut(s)	1311
SaqAI	TTAA	2 cut(s)	864, 1214
Sau3AI	GATC	5 cut(s)	425, 460, 628, 1315, 1391
Sau96I	GGNCC	1 cut(s)	1025
SchI	GAGTC	1 cut(s)	1007
ScrFI	CCNGG	3 cut(s)	274, 420, 1303
SduI	GDGCHC	2 cut(s)	1280, 1552
SexAI	ACCWGGT	1 cut(s)	418
SfaNI	GCATC	7 cut(s)	253, 413, 537, 1046, 1164, 1321, 1656
SfcI	CTRYAG	3 cut(s)	450, 1137, 1775
SfoI	GGCGCC	1 cut(s)	106
SmiMI	CAYNNNNRTG	1 cut(s)	1311
SmlI	CTYRAG	3 cut(s)	1213, 1551, 1718
SmoI	CTYRAG	3 cut(s)	1213, 1551, 1718
SsiI	CCGC	7 cut(s)	15, 118, 137, 164, 456, 1586, 1743
SspDI	GGCGCC	1 cut(s)	104
SspI	AATATT	1 cut(s)	820
SspMI	CTAG	1 cut(s)	432
StyD4I	CCNGG	3 cut(s)	272, 418, 1301
StyI	CCWWGG	3 cut(s)	156, 1413, 1437
TaaI	ACNGT	4 cut(s)	334, 493, 604, 1541
TaiI	ACGT	5 cut(s)	204, 281, 660, 1355, 1535
TaqI	TCGA	1 cut(s)	303
TatI	WGTACW	1 cut(s)	1598
TauI	GCSGC	4 cut(s)	17, 121, 458, 1745
TfiI	GAWTC	4 cut(s)	537, 739, 977, 1195
Tru1I	TTAA	2 cut(s)	864, 1214
Tru9I	TTAA	2 cut(s)	864, 1214
TscAI	CASTG	6 cut(s)	328, 483, 784, 1035, 1341, 1660
TseFI	GTSAC	1 cut(s)	1246
TseI	GCWGC	9 cut(s)	266, 401, 440, 661, 664, 1088, 1739, 1745, 1748
Tsp45I	GTSAC	1 cut(s)	1246
TspDTI	ATGAA	6 cut(s)	357, 828, 888, 1092, 1285, 1567
TspRI	CASTG	6 cut(s)	328, 483, 784, 1035, 1341, 1660
Van91I	CCANNNNNTGG	1 cut(s)	24
Vha464I	CTTAAG	1 cut(s)	1213
XapI	RAATTY	2 cut(s)	54, 1119
XceI	RCATGY	2 cut(s)	224, 321
XcmI	CCANNNNNNNNNTGG	1 cut(s)	878
XmiI	GTMKAC	1 cut(s)	1349
XmnI	GAANNNNTTC	1 cut(s)	1769
XspI	CTAG	1 cut(s)	432
ZraI	GACGTC	1 cut(s)	279

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.