pycom15g00400

Divergent CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0017732
Physical Location & Seq
Reverse (-)
255596 .. 256591
996 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
ATGAACCATTCTTCTCCTCCTTGTATACCAACAATCTCAATATCGGACCAGCAACACCTCCTTGAGAAACTTCAAGTCTTTAAGATCCAAGGCCGGGACAAACATGATCGAAAGGTTCTTCGCATCGTCGGAAAGTTCTTTCCAGCTCGAAGCGTGAGCAACGAGGCTGTGAACAAGTACTTGGAGGAGAAAATATTCCCAAAGTTAGGTGACAGAGCCTTCTTCACGGTGGTGTACGTTCACACAGATGTGAATCGCGGCGATAACTTTCCCGGAATTTCAACCCTGAGATCCATCTACGAGGCAATTCCGACCGCCATCAAGGATCGCCTCCAAGCCGTTTACTTTCTTCACCCAGGATTGCAGGCCAAGCTCTTCCTTGCCACCTTTGGTCGTCTCCTTTTCACCGGAGGGTTGTACAAGAAGCTAAAGTATGTGACAAGGCTGGAGTTTCTGTGGGTTCAGGTGCGGAGGAATGAGGTGGAAGTTCCGGATTTCGTGTGCGATTACGATGAGCAGCTGGAGTACCGTCCGATGATGGACTATGGATTAGAGAGTGATCACCCCAGAGTCTACGGGCCTTCCTCTTTGGCGTCTTCTACGACATCGTCCATGCTGGATTCGCCTGCTGTGTCCATGTACTCCATGCGTTGTATTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

221

Amino Acids

25.24

Weight (kDa)

8.88

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_2 PF13716 37 - 172 5.3e-35 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017732)

Species Orthologous Gene IDs
malus_domestica MD08G1006200.v1.1 MD15G1005600.v1.1
prunus_persica Prupe.1G358200_v2.0.a1
pyrus_communis pycom15g00400
rosa_chinensis RchiOBHm_Chr6g0303811
rosa_laevigata RLG00000011042
rosa_multiflora Rmu_sc0012878.1_g000003
rosa_roxburghii Rroxscaffold_7G00164390
rosa_rugosa Rorug06G0325000
rosa_samantha Rh6AG439300 Rh6BG463700
rosa_wichuraiana Rw6G038030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 25, 573
AccII CGCG 1 cut(s) 258
AccIII TCCGGA 1 cut(s) 490
AciI CCGC 3 cut(s) 258, 315, 469
AclWI GGATC 3 cut(s) 79, 285, 333
AcsI RAATTY 1 cut(s) 276
AcyI GRCGYC 1 cut(s) 593
AfaI GTAC 5 cut(s) 179, 236, 419, 527, 641
AfiI CCNNNNNNNGG 2 cut(s) 94, 206
AgsI TTSAA 2 cut(s) 74, 282
AjnI CCWGG 1 cut(s) 355
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AleI CACNNNNGTG 2 cut(s) 230, 248
AluBI AGCT 4 cut(s) 146, 373, 427, 520
AluI AGCT 4 cut(s) 146, 373, 427, 520
Alw26I GTCTC 1 cut(s) 401
AlwI GGATC 3 cut(s) 79, 285, 333
Aor13HI TCCGGA 1 cut(s) 490
AoxI GGCC 3 cut(s) 91, 366, 578
ApeKI GCWGC 1 cut(s) 517
ApoI RAATTY 1 cut(s) 276
Asp700I GAANNNNTTC 1 cut(s) 194
AspS9I GGNCC 2 cut(s) 46, 578
AsuC2I CCSGG 2 cut(s) 95, 273
AsuHPI GGTGA 4 cut(s) 221, 344, 397, 554
AvaII GGWCC 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 588
BbvI GCAGC 1 cut(s) 529
BccI CCATC 3 cut(s) 302, 326, 532
BceAI ACGGC 1 cut(s) 323
BciT130I CCWGG 1 cut(s) 357
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 2 cut(s) 95, 273
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 1 cut(s) 661
BisI GCNGC 2 cut(s) 259, 518
BlsI GCNGC 2 cut(s) 260, 519
BmcAI AGTACT 1 cut(s) 179
Bme1390I CCNGG 3 cut(s) 95, 273, 357
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 46, 578
BmrFI CCNGG 3 cut(s) 95, 273, 357
BmsI GCATC 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 588
BpmI CTGGAG 2 cut(s) 467, 542
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 2 cut(s) 95, 273
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 593
BsaJI CCNNGG 2 cut(s) 88, 355
BsaWI WCCGGW 2 cut(s) 407, 490
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 206
Bse8I GATNNNNATC 1 cut(s) 252
BseAI TCCGGA 1 cut(s) 490
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 2 cut(s) 88, 355
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 94, 206
BseMII CTCAG 1 cut(s) 278
BseRI GAGGAG 2 cut(s) 6, 200
BseXI GCAGC 1 cut(s) 529
Bsh1236I CGCG 1 cut(s) 258
Bsh1285I CGRYCG 1 cut(s) 315
BshFI GGCC 3 cut(s) 93, 368, 580
BsiEI CGRYCG 1 cut(s) 315
BsiSI CCGG 4 cut(s) 94, 273, 408, 491
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 94, 206
BsmAI GTCTC 1 cut(s) 401
BsmBI CGTCTC 1 cut(s) 401
BsmFI GGGAC 1 cut(s) 110
BsnI GGCC 3 cut(s) 93, 368, 580
Bsp13I TCCGGA 1 cut(s) 490
Bsp1407I TGTACA 1 cut(s) 417
Bsp143I GATC 5 cut(s) 84, 106, 290, 325, 559
BspACI CCGC 3 cut(s) 258, 315, 469
BspANI GGCC 3 cut(s) 93, 368, 580
BspCNI CTCAG 1 cut(s) 279
BspEI TCCGGA 1 cut(s) 490
BspFNI CGCG 1 cut(s) 258
BspPI GGATC 3 cut(s) 79, 285, 333
BspQI GCTCTTC 1 cut(s) 380
BsrGI TGTACA 1 cut(s) 417
BssECI CCNNGG 2 cut(s) 88, 355
BssMI GATC 5 cut(s) 84, 106, 290, 325, 559
BssNAI GTATAC 1 cut(s) 26
BssNI GRCGYC 1 cut(s) 593
BssT1I CCWWGG 1 cut(s) 88
Bst1107I GTATAC 1 cut(s) 26
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 2 cut(s) 229, 530
Bst6I CTCTTC 1 cut(s) 380
BstACI GRCGYC 1 cut(s) 593
BstAUI TGTACA 1 cut(s) 417
BstC8I GCNNGC 2 cut(s) 366, 627
BstDEI CTNAG 1 cut(s) 287
BstFNI CGCG 1 cut(s) 258
BstKTI GATC 5 cut(s) 87, 109, 293, 328, 562
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 5 cut(s) 84, 106, 290, 325, 559
BstMCI CGRYCG 1 cut(s) 315
BstMWI GCNNNNNNNGC 2 cut(s) 370, 622
BstNI CCWGG 1 cut(s) 357
BstSCI CCNGG 3 cut(s) 93, 271, 355
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 529
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 2 cut(s) 84, 290
BstYI RGATCY 2 cut(s) 84, 290
BstZ17I GTATAC 1 cut(s) 26
BsuRI GGCC 3 cut(s) 93, 368, 580
Cac8I GCNNGC 2 cut(s) 366, 627
Cfr13I GGNCC 2 cut(s) 46, 578
CseI GACGC 1 cut(s) 582
Csp6I GTAC 5 cut(s) 178, 235, 418, 526, 640
CviAII CATG 4 cut(s) 104, 613, 637, 646
CviQI GTAC 5 cut(s) 178, 235, 418, 526, 640
DdeI CTNAG 1 cut(s) 287
DpnI GATC 5 cut(s) 86, 108, 292, 327, 561
DpnII GATC 5 cut(s) 84, 106, 290, 325, 559
Eam1104I CTCTTC 1 cut(s) 380
EarI CTCTTC 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 88
ErhI CCWWGG 1 cut(s) 88
Esp3I CGTCTC 1 cut(s) 401
FaeI CATG 4 cut(s) 107, 616, 640, 649
FaiI YATR 7 cut(s) 26, 105, 435, 546, 614, 638, 647
FaqI GGGAC 1 cut(s) 110
FatI CATG 4 cut(s) 103, 612, 636, 645
FbaI TGATCA 1 cut(s) 559
FblI GTMKAC 2 cut(s) 25, 573
Fnu4HI GCNGC 2 cut(s) 259, 518
Fsp4HI GCNGC 2 cut(s) 259, 518
FspBI CTAG 1 cut(s) 661
GluI GCNGC 2 cut(s) 259, 518
GsuI CTGGAG 2 cut(s) 467, 542
HaeIII GGCC 3 cut(s) 93, 368, 580
HapII CCGG 4 cut(s) 94, 273, 408, 491
HgaI GACGC 1 cut(s) 582
Hin1I GRCGYC 1 cut(s) 593
Hin1II CATG 4 cut(s) 107, 616, 640, 649
HinfI GANTC 3 cut(s) 253, 570, 620
HpaII CCGG 4 cut(s) 94, 273, 408, 491
HphI GGTGA 4 cut(s) 221, 344, 397, 554
Hpy166II GTNNAC 6 cut(s) 26, 172, 235, 241, 343, 574
Hpy188I TCNGA 4 cut(s) 46, 131, 312, 534
Hpy188III TCNNGA 1 cut(s) 491
Hpy8I GTNNAC 6 cut(s) 26, 172, 235, 241, 343, 574
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 2 cut(s) 229, 591
HpyCH4III ACNGT 2 cut(s) 229, 530
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 1 cut(s) 364
HpyF10VI GCNNNNNNNGC 2 cut(s) 370, 622
HpyF3I CTNAG 1 cut(s) 287
HpySE526I ACGT 1 cut(s) 237
Hsp92I GRCGYC 1 cut(s) 593
Hsp92II CATG 4 cut(s) 107, 616, 640, 649
Kpn2I TCCGGA 1 cut(s) 490
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 5 cut(s) 84, 106, 290, 325, 559
LguI GCTCTTC 1 cut(s) 380
Lsp1109I GCAGC 1 cut(s) 529
LweI GCATC 1 cut(s) 132
MaeI CTAG 1 cut(s) 661
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 2 cut(s) 209, 436
MalI GATC 5 cut(s) 86, 108, 292, 327, 561
MboI GATC 5 cut(s) 84, 106, 290, 325, 559
MboII GAAGA 6 cut(s) 3, 110, 214, 341, 367, 588
MflI RGATCY 2 cut(s) 84, 290
MluCI AATT 2 cut(s) 276, 306
MlyI GAGTC 1 cut(s) 579
MmeI TCCRAC 2 cut(s) 109, 335
MroI TCCGGA 1 cut(s) 490
MroXI GAANNNNTTC 1 cut(s) 194
MseI TTAA 1 cut(s) 81
MslI CAYNNNNRTG 3 cut(s) 230, 246, 248
MspA1I CMGCKG 1 cut(s) 520
MspI CCGG 4 cut(s) 94, 273, 408, 491
MspR9I CCNGG 3 cut(s) 95, 273, 357
MvaI CCWGG 1 cut(s) 357
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 2 cut(s) 370, 622
NciI CCSGG 2 cut(s) 95, 273
NdeII GATC 5 cut(s) 84, 106, 290, 325, 559
NlaIII CATG 4 cut(s) 107, 616, 640, 649
NmuCI GTSAC 2 cut(s) 209, 436
OliI CACNNNNGTG 2 cut(s) 230, 248
PciSI GCTCTTC 1 cut(s) 380
PdmI GAANNNNTTC 1 cut(s) 194
PfeI GAWTC 2 cut(s) 253, 620
PflFI GACNNNGTC 1 cut(s) 607
PfoI TCCNGGA 1 cut(s) 271
PkrI GCNGC 2 cut(s) 260, 519
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspPI GGNCC 2 cut(s) 46, 578
PsuI RGATCY 2 cut(s) 84, 290
PsyI GACNNNGTC 1 cut(s) 607
PvuII CAGCTG 1 cut(s) 520
RsaI GTAC 5 cut(s) 179, 236, 419, 527, 641
RsaNI GTAC 5 cut(s) 178, 235, 418, 526, 640
RseI CAYNNNNRTG 3 cut(s) 230, 246, 248
SapI GCTCTTC 1 cut(s) 380
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 2 cut(s) 259, 518
Sau3AI GATC 5 cut(s) 84, 106, 290, 325, 559
Sau96I GGNCC 2 cut(s) 46, 578
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 1 cut(s) 579
ScrFI CCNGG 3 cut(s) 95, 273, 357
SfaNI GCATC 1 cut(s) 132
SinI GGWCC 1 cut(s) 46
SmiMI CAYNNNNRTG 3 cut(s) 230, 246, 248
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 2 cut(s) 276, 306
SsiI CCGC 3 cut(s) 258, 315, 469
SspI AATATT 1 cut(s) 195
SspMI CTAG 1 cut(s) 661
StyD4I CCNGG 3 cut(s) 93, 271, 355
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 2 cut(s) 229, 530
TaiI ACGT 1 cut(s) 240
TaqI TCGA 2 cut(s) 109, 148
TasI AATT 2 cut(s) 276, 306
TatI WGTACW 3 cut(s) 177, 417, 639
TauI GCSGC 1 cut(s) 261
TfiI GAWTC 2 cut(s) 253, 620
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TseFI GTSAC 2 cut(s) 209, 436
TseI GCWGC 1 cut(s) 517
Tsp45I GTSAC 2 cut(s) 209, 436
TspDTI ATGAA 1 cut(s) 17
Tth111I GACNNNGTC 1 cut(s) 607
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 1 cut(s) 276
XmiI GTMKAC 2 cut(s) 25, 573
XmnI GAANNNNTTC 1 cut(s) 194
XspI CTAG 1 cut(s) 661
ZrmI AGTACT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.