pycom15g00780

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010321
Physical Location & Seq
Reverse (-)
431869 .. 434908
3040 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 822 bp
ATGGATGTCCTTTGCCAGATTGGTTTAGATGGTATCCGTATGATCGATCCTGCTACCAGTCGGACATTGAGAATATATCCCCTCGAGACCGTTACAAGATGTGAAAAAACCGACTCATCTACTTTTGCCTTTTGGTCAAAGAGCTCTGTGGATATTGAGCCAAGGCGTATTAGATTGCAGTCAAATAGTTACACTACCAACACCCTTTTGGATACGGTGACTGCTGCAACTGTACAGCTTAAGGAAATGGGTGGAAGAATAAAGCCTACAGAATCCATAAGGCCAAGTGAACAGTCTACAGAGAGAAAGAAAGGATTAACCGATTGGATGAACATTATTAAACCGGGAAATGAGGAGAAAGATCATTGGGTCCCCGATGAAGCAGTTACAAAATGCACTGCGTGTCGGACAGATTTTGGGGCTTTTAACCGCAGGCATCACTGTCGGAACTGTGGAGACATTTTCTGCGACAAGTGTACCCATGGCAGAATTGCCTTAACTGCTGAGGAGAATGCTCCGCAGGTTCGAGTTTGTGACCGTTGCATGGCTGAAGTGACTCAGAGGCTCAGTAATGCTAAGGAAGCATCTAGTAAACCTGCAGGACTTCATAGTCACGACGATCTTGCCAAGAAGCTTCAGGAGGAGTTGGAAAGGAATCGCAAGGAATCTTCAGGTTCAAAGTCTGATGGATCTGGAAGGAGAATGAGAGAAGTTGCCTGTCCTACATGTACGGTCCACTTGCAGGTTCAAGTTCCCAGCACGGGGTCGGAGACCATTGAGTGTGGGGTTTGCCAGAATCCATTTCTTGTGAGTGCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYVE PTB

Protein Analysis

274

Amino Acids

30.47

Weight (kDa)

7.91

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FYVE PF01363 122 - 186 6.7e-21 FYVE zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 609
Acc36I ACCTGC 3 cut(s) 511, 604, 733
AccI GTMKAC 1 cut(s) 296
AciI CCGC 2 cut(s) 430, 518
AclWI GGATC 2 cut(s) 41, 697
AcuI CTGAAG 3 cut(s) 570, 620, 654
AdeI CACNNNGTG 1 cut(s) 402
AfaI GTAC 3 cut(s) 234, 478, 730
AfiI CCNNNNNNNGG 4 cut(s) 544, 742, 761, 762
AflII CTTAAG 1 cut(s) 239
AflIII ACRYGT 1 cut(s) 725
AgsI TTSAA 2 cut(s) 678, 749
AluBI AGCT 3 cut(s) 144, 238, 634
AluI AGCT 3 cut(s) 144, 238, 634
Alw21I GWGCWC 2 cut(s) 146, 817
Alw26I GTCTC 3 cut(s) 80, 450, 764
AlwI GGATC 2 cut(s) 41, 697
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 1 cut(s) 281
ApeKI GCWGC 1 cut(s) 224
AspS9I GGNCC 2 cut(s) 370, 733
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 2 cut(s) 370, 733
BaeI ACNNNNGTAYC 2 cut(s) 460, 493
BanII GRGCYC 1 cut(s) 146
Bbv12I GWGCWC 2 cut(s) 146, 817
BbvCI CCTCAGC 1 cut(s) 504
BbvI GCAGC 1 cut(s) 211
BccI CCATC 2 cut(s) 23, 680
BcgI CGANNNNNNTGC 4 cut(s) 425, 459, 605, 639
BciVI GTATCC 2 cut(s) 44, 205
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 3 cut(s) 80, 450, 764
BfaI CTAG 1 cut(s) 588
BfmI CTRYAG 3 cut(s) 267, 297, 597
BfrI CTTAAG 1 cut(s) 239
BfuAI ACCTGC 3 cut(s) 511, 604, 733
BfuI GTATCC 2 cut(s) 44, 205
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 2 cut(s) 370, 733
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 2 cut(s) 370, 733
BmiI GGNNCC 2 cut(s) 371, 372
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 2 cut(s) 445, 593
Bpu10I CCTNAGC 2 cut(s) 504, 576
BpuMI CCSGG 1 cut(s) 345
Bsa29I ATCGAT 1 cut(s) 45
BsaI GGTCTC 2 cut(s) 80, 764
BsaJI CCNNGG 2 cut(s) 161, 481
Bsc4I CCNNNNNNNGG 4 cut(s) 544, 742, 761, 762
Bse1I ACTGG 1 cut(s) 57
BseCI ATCGAT 1 cut(s) 45
BseDI CCNNGG 2 cut(s) 161, 481
BseGI GGATG 2 cut(s) 10, 333
BseLI CCNNNNNNNGG 4 cut(s) 544, 742, 761, 762
BseMII CTCAG 3 cut(s) 495, 572, 580
BseNI ACTGG 1 cut(s) 57
BseRI GAGGAG 3 cut(s) 368, 521, 656
BseXI GCAGC 1 cut(s) 211
BseYI CCCAGC 1 cut(s) 755
BshFI GGCC 1 cut(s) 283
BshVI ATCGAT 1 cut(s) 45
BsiHKAI GWGCWC 2 cut(s) 146, 817
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 1 cut(s) 344
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 4 cut(s) 544, 742, 761, 762
BsmAI GTCTC 3 cut(s) 80, 450, 764
BsmFI GGGAC 1 cut(s) 356
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 1 cut(s) 283
Bso31I GGTCTC 2 cut(s) 80, 764
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 2 cut(s) 146, 817
Bsp1407I TGTACA 1 cut(s) 232
Bsp143I GATC 5 cut(s) 42, 46, 361, 619, 689
Bsp19I CCATGG 1 cut(s) 481
BspACI CCGC 2 cut(s) 430, 518
BspANI GGCC 1 cut(s) 283
BspCNI CTCAG 3 cut(s) 496, 571, 579
BspDI ATCGAT 1 cut(s) 45
BspLI GGNNCC 2 cut(s) 371, 372
BspMAI CTGCAG 1 cut(s) 601
BspMI ACCTGC 3 cut(s) 511, 604, 733
BspPI GGATC 2 cut(s) 41, 697
BspTI CTTAAG 1 cut(s) 239
BspTNI GGTCTC 2 cut(s) 80, 764
BsrGI TGTACA 1 cut(s) 232
BsrI ACTGG 1 cut(s) 57
BssECI CCNNGG 2 cut(s) 161, 481
BssMI GATC 5 cut(s) 42, 46, 361, 619, 689
BssT1I CCWWGG 2 cut(s) 161, 481
Bst4CI ACNGT 8 cut(s) 91, 217, 232, 294, 443, 452, 539, 733
BstAFI CTTAAG 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 232
BstC8I GCNNGC 1 cut(s) 434
BstDEI CTNAG 4 cut(s) 504, 558, 566, 576
BstDSI CCRYGG 1 cut(s) 481
BstF5I GGATG 2 cut(s) 10, 333
BstKTI GATC 5 cut(s) 45, 49, 364, 622, 692
BstMAI GTCTC 3 cut(s) 80, 450, 764
BstMBI GATC 5 cut(s) 42, 46, 361, 619, 689
BstMWI GCNNNNNNNGC 2 cut(s) 500, 581
BstNSI RCATGY 1 cut(s) 729
BstSCI CCNGG 1 cut(s) 343
BstSFI CTRYAG 3 cut(s) 267, 297, 597
BstV1I GCAGC 1 cut(s) 211
BstX2I RGATCY 1 cut(s) 689
BstYI RGATCY 1 cut(s) 689
Bsu15I ATCGAT 1 cut(s) 45
BsuI GTATCC 2 cut(s) 44, 205
BsuRI GGCC 1 cut(s) 283
BsuTUI ATCGAT 1 cut(s) 45
BtgI CCRYGG 1 cut(s) 481
BtsCI GGATG 2 cut(s) 10, 333
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 2 cut(s) 396, 439
BveI ACCTGC 3 cut(s) 511, 604, 733
Cac8I GCNNGC 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 370, 733
ClaI ATCGAT 1 cut(s) 45
Csp6I GTAC 3 cut(s) 233, 477, 729
CviAII CATG 3 cut(s) 482, 544, 726
CviJI RGCY 9 cut(s) 144, 160, 238, 265, 283, 422, 548, 565, 634
CviKI_1 RGCY 9 cut(s) 144, 160, 238, 265, 283, 422, 548, 565, 634
CviQI GTAC 3 cut(s) 233, 477, 729
DdeI CTNAG 4 cut(s) 504, 558, 566, 576
DpnI GATC 5 cut(s) 44, 48, 363, 621, 691
DpnII GATC 5 cut(s) 42, 46, 361, 619, 689
DraIII CACNNNGTG 1 cut(s) 402
DrdI GACNNNNNNGTC 1 cut(s) 609
DseDI GACNNNNNNGTC 1 cut(s) 609
Ecl136II GAGCTC 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 161, 481
Eco24I GRGCYC 1 cut(s) 146
Eco31I GGTCTC 2 cut(s) 80, 764
Eco47I GGWCC 2 cut(s) 370, 733
Eco53kI GAGCTC 1 cut(s) 144
Eco57I CTGAAG 3 cut(s) 570, 620, 654
Eco88I CYCGRG 1 cut(s) 83
EcoICRI GAGCTC 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 370
EcoT14I CCWWGG 2 cut(s) 161, 481
EcoT38I GRGCYC 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 161, 481
FaeI CATG 3 cut(s) 485, 547, 729
FaiI YATR 7 cut(s) 41, 76, 278, 483, 545, 609, 727
FaqI GGGAC 1 cut(s) 356
FatI CATG 3 cut(s) 481, 543, 725
FblI GTMKAC 1 cut(s) 296
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 2 cut(s) 17, 340
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 1 cut(s) 588
GluI GCNGC 1 cut(s) 225
GsaI CCCAGC 1 cut(s) 759
HaeIII GGCC 1 cut(s) 283
HapII CCGG 1 cut(s) 344
Hin1II CATG 3 cut(s) 485, 547, 729
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 6 cut(s) 113, 272, 556, 655, 665, 796
HpaII CCGG 1 cut(s) 344
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 5 cut(s) 290, 297, 477, 593, 736
Hpy188I TCNGA 6 cut(s) 63, 408, 447, 561, 685, 769
Hpy188III TCNNGA 4 cut(s) 85, 614, 638, 693
Hpy8I GTNNAC 5 cut(s) 290, 297, 477, 593, 736
Hpy99I CGWCG 1 cut(s) 620
HpyAV CCTTC 1 cut(s) 690
HpyCH4III ACNGT 8 cut(s) 91, 217, 232, 294, 443, 452, 539, 733
HpyCH4V TGCA 6 cut(s) 178, 227, 396, 543, 599, 742
HpyF10VI GCNNNNNNNGC 2 cut(s) 500, 581
HpyF3I CTNAG 4 cut(s) 504, 558, 566, 576
Hsp92II CATG 3 cut(s) 485, 547, 729
KflI GGGWCCC 1 cut(s) 370
Kzo9I GATC 5 cut(s) 42, 46, 361, 619, 689
LmnI GCTCC 1 cut(s) 520
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 2 cut(s) 445, 593
MaeI CTAG 1 cut(s) 588
MaeIII GTNAC 7 cut(s) 91, 188, 217, 385, 533, 553, 611
MalI GATC 5 cut(s) 44, 48, 363, 621, 691
MboI GATC 5 cut(s) 42, 46, 361, 619, 689
MboII GAAGA 2 cut(s) 267, 660
MflI RGATCY 1 cut(s) 689
MhlI GDGCHC 2 cut(s) 146, 817
MluCI AATT 1 cut(s) 489
MlyI GAGTC 2 cut(s) 107, 550
MmeI TCCRAC 5 cut(s) 41, 386, 425, 627, 747
MnlI CCTC 5 cut(s) 92, 346, 499, 555, 634
MseI TTAA 5 cut(s) 240, 317, 339, 426, 497
MspCI CTTAAG 1 cut(s) 239
MspI CCGG 1 cut(s) 344
MspR9I CCNGG 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 517
MwoI GCNNNNNNNGC 2 cut(s) 500, 581
NciI CCSGG 1 cut(s) 345
NcoI CCATGG 1 cut(s) 481
NdeII GATC 5 cut(s) 42, 46, 361, 619, 689
NlaIII CATG 3 cut(s) 485, 547, 729
NlaIV GGNNCC 2 cut(s) 371, 372
NmuCI GTSAC 4 cut(s) 217, 533, 553, 611
NspI RCATGY 1 cut(s) 729
PaeR7I CTCGAG 1 cut(s) 83
PciI ACATGT 1 cut(s) 725
PctI GAATGC 1 cut(s) 517
PfeI GAWTC 4 cut(s) 272, 655, 665, 796
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 2 cut(s) 107, 550
PpsI GAGTC 2 cut(s) 107, 550
PpuMI RGGWCCY 1 cut(s) 370
PscI ACATGT 1 cut(s) 725
Psp124BI GAGCTC 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 370
PspFI CCCAGC 1 cut(s) 755
PspN4I GGNNCC 2 cut(s) 371, 372
PspPI GGNCC 2 cut(s) 370, 733
PspPPI RGGWCCY 1 cut(s) 370
PstI CTGCAG 1 cut(s) 601
PsuI RGATCY 1 cut(s) 689
RsaI GTAC 3 cut(s) 234, 478, 730
RsaNI GTAC 3 cut(s) 233, 477, 729
SacI GAGCTC 1 cut(s) 146
SaqAI TTAA 5 cut(s) 240, 317, 339, 426, 497
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 5 cut(s) 42, 46, 361, 619, 689
Sau96I GGNCC 2 cut(s) 370, 733
SbfI CCTGCAGG 1 cut(s) 601
SchI GAGTC 2 cut(s) 107, 550
ScrFI CCNGG 1 cut(s) 345
SdaI CCTGCAGG 1 cut(s) 601
SduI GDGCHC 2 cut(s) 146, 817
SetI ASST 7 cut(s) 146, 240, 525, 598, 636, 676, 747
SfaNI GCATC 2 cut(s) 445, 593
SfcI CTRYAG 3 cut(s) 267, 297, 597
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 2 cut(s) 370, 733
SlaI CTCGAG 1 cut(s) 83
SmlI CTYRAG 2 cut(s) 83, 239
SmoI CTYRAG 2 cut(s) 83, 239
Sse8387I CCTGCAGG 1 cut(s) 601
Sse9I AATT 1 cut(s) 489
SsiI CCGC 2 cut(s) 430, 518
SspMI CTAG 1 cut(s) 588
SstI GAGCTC 1 cut(s) 146
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 2 cut(s) 161, 481
TaaI ACNGT 8 cut(s) 91, 217, 232, 294, 443, 452, 539, 733
TaqI TCGA 3 cut(s) 45, 84, 526
TasI AATT 1 cut(s) 489
TatI WGTACW 1 cut(s) 232
TfiI GAWTC 4 cut(s) 272, 655, 665, 796
Tru1I TTAA 5 cut(s) 240, 317, 339, 426, 497
Tru9I TTAA 5 cut(s) 240, 317, 339, 426, 497
TscAI CASTG 2 cut(s) 403, 446
TseFI GTSAC 4 cut(s) 217, 533, 553, 611
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 4 cut(s) 217, 533, 553, 611
TspDTI ATGAA 3 cut(s) 344, 393, 596
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 2 cut(s) 403, 446
Vha464I CTTAAG 1 cut(s) 239
VpaK11BI GGWCC 2 cut(s) 370, 733
XceI RCATGY 1 cut(s) 729
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XhoI CTCGAG 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 296
XspI CTAG 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.