Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0009949

Physical Location & Seq

Genomic Coordinates Forward (+)

1009947 .. 1012710

Gene Structure 2764 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1815 bp

ATGCGCCATGCATACAATGCTTTCCATATATTCCAAGAAATCGGAATAAAGCACTCTTTTGCGTCACGGCGTAATCCTTTCCTCCAATGCTCAAGTACTGAGGGATCTGACAAGAAATCCATATCAGTTGGGCCGTGGGGAGGTCACAACGGGCTCATTTGGGATGACAGAGTTCGCTCAACTGTGAAGCAGCTGGTGATAGCTCATGGAGCTGCCATTGACTCTATCCAGATTGAATATGATGAGAGAGGAAGCTCAGTTTGGTCAGACAAGCATGGTGGAAATGGAGGCTGGAAAACTGACAAGGTGAAGCTTGCTTCTCCAGAAGAATTTTTAACTTCAATCGAAGGATATTACGGTAAGATAAGCGAATGGGGACCGATCACAGTTCGATCTCTCACTTTCAAGAGCAACAAAAGAACTTATGGACCGTTTGGGGTCGAACAAGGAACCTATTTTTCACTCCAGGAGACAGCTTCGAATGTTAAGATTGTTGGGTTCCATGGCATGTCTGGCCGGTATCTTGATTCAATAGGCGCTTACGTAAAGCCTATTGATCAGAATGAACAAAACCAACACGCGAAAACTCTGCTTCGGACACCGTTCAATTATCCGACTATTGGTACTGATCAGAACCTTGCTGGCTACTCACTAATCCAAAACTGTAATGTGCTTTTTGCTATAACGCCGAAGGATGACTCCACTAGTAAACCAGCTGCAGTACCAGTACCCAAAAAGCTCTCAGGGCAGTTTTCTGATTCCGAAACAAGTGATGATGTAGGAACCAAACATAAGCTGACGACGAATATTGAGAAGTTTCCCTCAAAAGTTGAAGGGATGGTAGCGTATGGCCCCTTCGGAGGGACGGGTGGTGCTGCATTTGATGATGGAATTTATTCGGGGATTAGACAAATCAAGTTATCAAGAAACATCGGTGTTGTATACATAAAGGTTCAGTATGACCACAATGGTGAAGCTGTTTGGGGAGGCAGACATGGCGGGACTGGAGGATTTAAAAGTGACAAGATAGTTTTTGATTACCCGAACGAGATCTTGACGCATATAACAGGAACATTTGCACCTACAAAGGTCATGGGGACTAGTGTTATCAAGTCCCTCACTTTCCACACCACGAAGAAGCAGCATGGGCCGTATGGAGAAGAACAAGGAGCCTGTTTCACAACCAAGCTTAAAGAAGGGAAAATCGTCGGAATTCATGGGAGGAGTGGTCTGTTCTTAGATGCTCTTGGGGTCCATGCCATAGAAGGAAAGGTCCATATTGTAGAGACAAAGACACCAACTCTCACAAATACTCCTAATATTCCCGACGGTCACACTGACCACAAAAATAAGACACCAACTGTCACAAATACTCCTAATACTTTCACTGCAATGATCCCAAAGGAGCATGCAGGGGCAATTACTGAGATAGTTAATCCTCACTGGACAAATAAACTACTAATGACAAACAGAGGAAAAGTCGAAGAGGTTGCTTATGGGGTGATAAAAGAACCAGCTCCGTGTGGACCGGGACCTTGGGGCGGAGATGGAGGTAGAGCATGGGATGATGGAGTGTTTTCCGGGATTAGTCAGATTCATTTGACAAGAGAAGCAGAAGGCATTTGCTCAGTGCAGATTGAGTATGATCGAAATGGTCAATTCATTTGGTCTGCTAAGCATGGAGGCAATGGAGGAACTTCCCCACATAGAGTACGAATGCTTTATCAACATCTTCATCAATTTGATTGTTTTTTTTTTTTTTTTCGCGATATCATAAACAACTATAATCTTAGAATCTACAGAAACGAGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Jacalin

Protein Analysis

605

Amino Acids

66.52

Weight (kDa)

8.71

Isoelectric Point (pI)

31.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Jacalin	PF01419	43 - 181	4.9e-45	Jacalin-like lectin domain
Jacalin	PF01419	284 - 419	3.8e-38	Jacalin-like lectin domain
Jacalin	PF01419	511 - 570	1.6e-13	Jacalin-like lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009949)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19715 AT1G19715 AT1G19715
fragaria_vesca	FvH4_2g39220
malus_domestica	MD08G1023700.v1.1 MD15G1021200.v1.1
prunus_persica	Prupe.1G227800_v2.0.a1 Prupe.1G372900_v2.0.a1 Prupe.1G372900_v2.0.a1
pyrus_communis	pycom08g01850 pycom15g01750
rosa_chinensis	RchiOBHm_Chr6g0301821
rosa_laevigata	RLG00000011217
rosa_multiflora	Rmu_sc0000407.1_g000013
rosa_roxburghii	Rroxscaffold_7G00166370
rosa_rugosa	Rorug06G0310100
rosa_samantha	Rh6AG421800 Rh6BG428500 Rh6CG435100 Rh6DG421900
rosa_wichuraiana	Rw6G036530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	942
AccII	CGCG	2 cut(s)	581, 1767
AciI	CCGC	2 cut(s)	999, 1542
AclWI	GGATC	2 cut(s)	112, 1390
AcoI	YGGCCR	1 cut(s)	514
AcsI	RAATTY	3 cut(s)	329, 891, 1212
AfaI	GTAC	5 cut(s)	97, 625, 723, 729, 1713
AfiI	CCNNNNNNNGG	5 cut(s)	140, 620, 860, 861, 1541
AgsI	TTSAA	6 cut(s)	236, 342, 406, 531, 607, 833
AhlI	ACTAGT	2 cut(s)	704, 1100
AjnI	CCWGG	1 cut(s)	465
AjuI	GAANNNNNNNTTGG	2 cut(s)	244, 276
AleI	CACNNNNGTG	1 cut(s)	969
Alw26I	GTCTC	2 cut(s)	464, 1280
AlwI	GGATC	2 cut(s)	112, 1390
AoxI	GGCC	4 cut(s)	131, 514, 850, 1148
ApeKI	GCWGC	5 cut(s)	190, 212, 716, 875, 1141
ApoI	RAATTY	3 cut(s)	329, 891, 1212
Asp700I	GAANNNNTTC	1 cut(s)	895
AspLEI	GCGC	2 cut(s)	6, 539
AspS9I	GGNCC	9 cut(s)	131, 377, 428, 851, 1148, 1252, 1273, 1526, 1532
AsuC2I	CCSGG	2 cut(s)	1530, 1582
AsuHPI	GGTGA	4 cut(s)	208, 319, 983, 1513
AsuII	TTCGAA	1 cut(s)	479
AvaII	GGWCC	6 cut(s)	377, 428, 1252, 1273, 1526, 1532
BanII	GRGCYC	1 cut(s)	156
BarI	GAAGNNNNNNTAC	2 cut(s)	839, 871
BbvI	GCAGC	5 cut(s)	199, 202, 703, 862, 1153
BccI	CCATC	4 cut(s)	832, 881, 1541, 1562
BceAI	ACGGC	3 cut(s)	83, 118, 1135
BcgI	CGANNNNNNTGC	4 cut(s)	571, 605, 1472, 1506
BciT130I	CCWGG	1 cut(s)	467
BclI	TGATCA	2 cut(s)	556, 628
BcnI	CCSGG	2 cut(s)	1530, 1582
BcoDI	GTCTC	2 cut(s)	464, 1280
BcuI	ACTAGT	2 cut(s)	704, 1100
BfaI	CTAG	2 cut(s)	705, 1101
BfmI	CTRYAG	2 cut(s)	717, 1798
BfoI	RGCGCY	1 cut(s)	540
BglII	AGATCT	1 cut(s)	1050
BisI	GCNGC	5 cut(s)	191, 213, 717, 876, 1142
BlpI	GCTNAGC	1 cut(s)	1674
BlsI	GCNGC	5 cut(s)	192, 214, 718, 877, 1143
BmcAI	AGTACT	1 cut(s)	97
Bme1390I	CCNGG	3 cut(s)	467, 1530, 1582
Bme18I	GGWCC	6 cut(s)	377, 428, 1252, 1273, 1526, 1532
BmgT120I	GGNCC	9 cut(s)	131, 377, 428, 851, 1148, 1252, 1273, 1526, 1532
BmiI	GGNNCC	8 cut(s)	378, 451, 500, 784, 853, 1171, 1253, 1533
BmrFI	CCNGG	3 cut(s)	467, 1530, 1582
BmsI	GCATC	1 cut(s)	1231
BpmI	CTGGAG	3 cut(s)	306, 449, 1026
Bpu1102I	GCTNAGC	1 cut(s)	1674
Bpu14I	TTCGAA	1 cut(s)	479
BpuEI	CTTGAG	1 cut(s)	76
BpuMI	CCSGG	2 cut(s)	1530, 1582
BsaAI	YACGTR	1 cut(s)	544
BsaJI	CCNNGG	3 cut(s)	134, 502, 1535
BsaXI	ACNNNNNCTCC	6 cut(s)	1213, 1243, 1297, 1327, 1357, 1387
Bsc4I	CCNNNNNNNGG	5 cut(s)	140, 620, 860, 861, 1541
Bse118I	RCCGGY	1 cut(s)	516
Bse1I	ACTGG	3 cut(s)	725, 1009, 1448
Bse3DI	GCAATG	2 cut(s)	1398, 1693
BseBI	CCWGG	1 cut(s)	467
BseDI	CCNNGG	3 cut(s)	134, 502, 1535
BseGI	GGATG	4 cut(s)	169, 700, 843, 1570
BseLI	CCNNNNNNNGG	5 cut(s)	140, 620, 860, 861, 1541
BseMI	GCAATG	2 cut(s)	1398, 1693
BseMII	CTCAG	5 cut(s)	90, 270, 756, 1416, 1641
BseNI	ACTGG	3 cut(s)	725, 1009, 1448
BseRI	GAGGAG	1 cut(s)	1237
BseXI	GCAGC	5 cut(s)	199, 202, 703, 862, 1153
BsgI	GTGCAG	1 cut(s)	1652
Bsh1236I	CGCG	2 cut(s)	581, 1767
BshFI	GGCC	4 cut(s)	133, 516, 852, 1150
BsiSI	CCGG	3 cut(s)	517, 1529, 1581
BslFI	GGGAC	6 cut(s)	390, 877, 1015, 1099, 1111, 1545
BslI	CCNNNNNNNGG	5 cut(s)	140, 620, 860, 861, 1541
BsmAI	GTCTC	2 cut(s)	464, 1280
BsmFI	GGGAC	6 cut(s)	390, 877, 1015, 1099, 1111, 1545
BsmI	GAATGC	1 cut(s)	1722
BsnI	GGCC	4 cut(s)	133, 516, 852, 1150
Bsp119I	TTCGAA	1 cut(s)	479
Bsp1286I	GDGCHC	1 cut(s)	156
Bsp143I	GATC	8 cut(s)	104, 381, 392, 556, 628, 1050, 1395, 1645
Bsp1720I	GCTNAGC	1 cut(s)	1674
Bsp19I	CCATGG	1 cut(s)	502
Bsp68I	TCGCGA	1 cut(s)	1767
BspACI	CCGC	2 cut(s)	999, 1542
BspANI	GGCC	4 cut(s)	133, 516, 852, 1150
BspCNI	CTCAG	5 cut(s)	91, 269, 755, 1417, 1640
BspFNI	CGCG	2 cut(s)	581, 1767
BspLI	GGNNCC	8 cut(s)	378, 451, 500, 784, 853, 1171, 1253, 1533
BspMAI	CTGCAG	1 cut(s)	721
BspPI	GGATC	2 cut(s)	112, 1390
BspT104I	TTCGAA	1 cut(s)	479
BsrDI	GCAATG	2 cut(s)	1398, 1693
BsrFI	RCCGGY	1 cut(s)	516
BsrI	ACTGG	3 cut(s)	725, 1009, 1448
BssAI	RCCGGY	1 cut(s)	516
BssECI	CCNNGG	3 cut(s)	134, 502, 1535
BssMI	GATC	8 cut(s)	104, 381, 392, 556, 628, 1050, 1395, 1645
BssNAI	GTATAC	1 cut(s)	943
BssT1I	CCWWGG	2 cut(s)	502, 1535
Bst1107I	GTATAC	1 cut(s)	943
Bst2UI	CCWGG	1 cut(s)	467
Bst4CI	ACNGT	8 cut(s)	184, 359, 388, 432, 603, 665, 1331, 1363
Bst6I	CTCTTC	1 cut(s)	1479
BstAPI	GCANNNNNTGC	1 cut(s)	17
BstBAI	YACGTR	1 cut(s)	544
BstBI	TTCGAA	1 cut(s)	479
BstC8I	GCNNGC	3 cut(s)	315, 643, 1410
BstDEI	CTNAG	8 cut(s)	99, 256, 742, 1237, 1425, 1627, 1674, 1790
BstDSI	CCRYGG	2 cut(s)	134, 502
BstF5I	GGATG	4 cut(s)	169, 700, 843, 1570
BstFNI	CGCG	2 cut(s)	581, 1767
BstH2I	RGCGCY	1 cut(s)	540
BstHHI	GCGC	2 cut(s)	6, 539
BstKTI	GATC	8 cut(s)	107, 384, 395, 559, 631, 1053, 1398, 1648
BstMAI	GTCTC	2 cut(s)	464, 1280
BstMBI	GATC	8 cut(s)	104, 381, 392, 556, 628, 1050, 1395, 1645
BstMWI	GCNNNNNNNGC	6 cut(s)	17, 209, 513, 745, 996, 1147
BstNI	CCWGG	1 cut(s)	467
BstNSI	RCATGY	2 cut(s)	511, 1412
BstSCI	CCNGG	3 cut(s)	465, 1528, 1580
BstSFI	CTRYAG	2 cut(s)	717, 1798
BstSNI	TACGTA	1 cut(s)	544
BstUI	CGCG	2 cut(s)	581, 1767
BstV1I	GCAGC	5 cut(s)	199, 202, 703, 862, 1153
BstX2I	RGATCY	2 cut(s)	104, 1050
BstYI	RGATCY	2 cut(s)	104, 1050
BstZ17I	GTATAC	1 cut(s)	943
BsuRI	GGCC	4 cut(s)	133, 516, 852, 1150
BtgI	CCRYGG	2 cut(s)	134, 502
BtsCI	GGATG	4 cut(s)	169, 700, 843, 1570
BtsI	GCAGTG	1 cut(s)	1386
BtsIMutI	CAGTG	4 cut(s)	1335, 1386, 1441, 1635
BtuMI	TCGCGA	1 cut(s)	1767
Cac8I	GCNNGC	3 cut(s)	315, 643, 1410
CfoI	GCGC	2 cut(s)	6, 539
Cfr10I	RCCGGY	1 cut(s)	516
Cfr13I	GGNCC	9 cut(s)	131, 377, 428, 851, 1148, 1252, 1273, 1526, 1532
CseI	GACGC	2 cut(s)	51, 1066
Csp6I	GTAC	5 cut(s)	96, 624, 722, 728, 1712
CspCI	CAANNNNNGTGG	2 cut(s)	259, 294
CviQI	GTAC	5 cut(s)	96, 624, 722, 728, 1712
DdeI	CTNAG	8 cut(s)	99, 256, 742, 1237, 1425, 1627, 1674, 1790
DpnI	GATC	8 cut(s)	106, 383, 394, 558, 630, 1052, 1397, 1647
DpnII	GATC	8 cut(s)	104, 381, 392, 556, 628, 1050, 1395, 1645
DraI	TTTAAA	1 cut(s)	1015
EaeI	YGGCCR	1 cut(s)	514
Eam1104I	CTCTTC	1 cut(s)	1479
EarI	CTCTTC	1 cut(s)	1479
EciI	GGCGGA	1 cut(s)	1557
Eco105I	TACGTA	1 cut(s)	544
Eco130I	CCWWGG	2 cut(s)	502, 1535
Eco24I	GRGCYC	1 cut(s)	156
Eco32I	GATATC	1 cut(s)	1771
Eco47I	GGWCC	6 cut(s)	377, 428, 1252, 1273, 1526, 1532
EcoO109I	RGGNCCY	1 cut(s)	1532
EcoRI	GAATTC	1 cut(s)	1212
EcoRII	CCWGG	1 cut(s)	465
EcoRV	GATATC	1 cut(s)	1771
EcoT14I	CCWWGG	2 cut(s)	502, 1535
EcoT22I	ATGCAT	1 cut(s)	13
EcoT38I	GRGCYC	1 cut(s)	156
ErhI	CCWWGG	2 cut(s)	502, 1535
FaqI	GGGAC	6 cut(s)	390, 877, 1015, 1099, 1111, 1545
FauI	CCCGC	1 cut(s)	992
FbaI	TGATCA	2 cut(s)	556, 628
FblI	GTMKAC	1 cut(s)	942
Fnu4HI	GCNGC	5 cut(s)	191, 213, 717, 876, 1142
FokI	GGATG	4 cut(s)	176, 707, 850, 1577
FriOI	GRGCYC	1 cut(s)	156
Fsp4HI	GCNGC	5 cut(s)	191, 213, 717, 876, 1142
FspBI	CTAG	2 cut(s)	705, 1101
GlaI	GCGC	2 cut(s)	5, 538
GluI	GCNGC	5 cut(s)	191, 213, 717, 876, 1142
GsuI	CTGGAG	3 cut(s)	306, 449, 1026
HaeII	RGCGCY	1 cut(s)	540
HaeIII	GGCC	4 cut(s)	133, 516, 852, 1150
HapII	CCGG	3 cut(s)	517, 1529, 1581
HgaI	GACGC	2 cut(s)	51, 1066
HhaI	GCGC	2 cut(s)	6, 539
Hin6I	GCGC	2 cut(s)	4, 537
HinP1I	GCGC	2 cut(s)	4, 537
HindIII	AAGCTT	2 cut(s)	311, 1187
HinfI	GANTC	6 cut(s)	221, 527, 698, 758, 1594, 1794
HpaII	CCGG	3 cut(s)	517, 1529, 1581
HphI	GGTGA	4 cut(s)	208, 319, 983, 1513
Hpy166II	GTNNAC	3 cut(s)	710, 943, 1526
Hpy188III	TCNNGA	8 cut(s)	229, 323, 406, 524, 924, 1054, 1325, 1766
Hpy8I	GTNNAC	3 cut(s)	710, 943, 1526
Hpy99I	CGWCG	3 cut(s)	805, 1211, 1331
HpyAV	CCTTC	7 cut(s)	341, 685, 827, 865, 1190, 1259, 1610
HpyCH4III	ACNGT	8 cut(s)	184, 359, 388, 432, 603, 665, 1331, 1363
HpyCH4IV	ACGT	1 cut(s)	543
HpyCH4V	TGCA	7 cut(s)	11, 719, 878, 1079, 1391, 1412, 1633
HpyF10VI	GCNNNNNNNGC	6 cut(s)	17, 209, 513, 745, 996, 1147
HpyF3I	CTNAG	8 cut(s)	99, 256, 742, 1237, 1425, 1627, 1674, 1790
HpySE526I	ACGT	1 cut(s)	543
HspAI	GCGC	2 cut(s)	4, 537
Ksp22I	TGATCA	2 cut(s)	556, 628
Kzo9I	GATC	8 cut(s)	104, 381, 392, 556, 628, 1050, 1395, 1645
LmnI	GCTCC	4 cut(s)	209, 1169, 1405, 1522
Lsp1109I	GCAGC	5 cut(s)	199, 202, 703, 862, 1153
LweI	GCATC	1 cut(s)	1231
MaeI	CTAG	2 cut(s)	705, 1101
MaeII	ACGT	1 cut(s)	543
MaeIII	GTNAC	5 cut(s)	63, 143, 1019, 1331, 1363
MalI	GATC	8 cut(s)	106, 383, 394, 558, 630, 1052, 1397, 1647
MboI	GATC	8 cut(s)	104, 381, 392, 556, 628, 1050, 1395, 1645
MboII	GAAGA	5 cut(s)	338, 1147, 1172, 1496, 1724
MflI	RGATCY	2 cut(s)	104, 1050
MhlI	GDGCHC	1 cut(s)	156
MluCI	AATT	7 cut(s)	329, 607, 891, 1212, 1419, 1658, 1739
MlyI	GAGTC	2 cut(s)	215, 692
MmeI	TCCRAC	2 cut(s)	638, 1189
Mph1103I	ATGCAT	1 cut(s)	13
MroXI	GAANNNNTTC	1 cut(s)	895
MseI	TTAA	5 cut(s)	335, 486, 1014, 1191, 1434
MslI	CAYNNNNRTG	2 cut(s)	969, 1391
MspA1I	CMGCKG	2 cut(s)	193, 716
MspI	CCGG	3 cut(s)	517, 1529, 1581
MspR9I	CCNGG	3 cut(s)	467, 1530, 1582
Mva1269I	GAATGC	1 cut(s)	1722
MvaI	CCWGG	1 cut(s)	467
MvnI	CGCG	2 cut(s)	581, 1767
MwoI	GCNNNNNNNGC	6 cut(s)	17, 209, 513, 745, 996, 1147
NciI	CCSGG	2 cut(s)	1530, 1582
NcoI	CCATGG	1 cut(s)	502
NdeII	GATC	8 cut(s)	104, 381, 392, 556, 628, 1050, 1395, 1645
NlaIV	GGNNCC	8 cut(s)	378, 451, 500, 784, 853, 1171, 1253, 1533
NmuCI	GTSAC	5 cut(s)	63, 143, 1019, 1331, 1363
NruI	TCGCGA	1 cut(s)	1767
NsiI	ATGCAT	1 cut(s)	13
NspI	RCATGY	2 cut(s)	511, 1412
NspV	TTCGAA	1 cut(s)	479
OliI	CACNNNNGTG	1 cut(s)	969
PaeI	GCATGC	1 cut(s)	1412
PctI	GAATGC	1 cut(s)	1722
PdmI	GAANNNNTTC	1 cut(s)	895
PfeI	GAWTC	4 cut(s)	527, 758, 1594, 1794
PfoI	TCCNGGA	2 cut(s)	465, 1580
PkrI	GCNGC	5 cut(s)	192, 214, 718, 877, 1143
PleI	GAGTC	2 cut(s)	215, 692
PpsI	GAGTC	2 cut(s)	215, 692
Ppu21I	YACGTR	1 cut(s)	544
PpuMI	RGGWCCY	1 cut(s)	1532
Psp5II	RGGWCCY	1 cut(s)	1532
Psp6I	CCWGG	1 cut(s)	465
PspGI	CCWGG	1 cut(s)	465
PspN4I	GGNNCC	8 cut(s)	378, 451, 500, 784, 853, 1171, 1253, 1533
PspPI	GGNCC	9 cut(s)	131, 377, 428, 851, 1148, 1252, 1273, 1526, 1532
PspPPI	RGGWCCY	1 cut(s)	1532
PstI	CTGCAG	1 cut(s)	721
PsuI	RGATCY	2 cut(s)	104, 1050
PvuII	CAGCTG	2 cut(s)	193, 716
RruI	TCGCGA	1 cut(s)	1767
RsaI	GTAC	5 cut(s)	97, 625, 723, 729, 1713
RsaNI	GTAC	5 cut(s)	96, 624, 722, 728, 1712
RseI	CAYNNNNRTG	2 cut(s)	969, 1391
SaqAI	TTAA	5 cut(s)	335, 486, 1014, 1191, 1434
SatI	GCNGC	5 cut(s)	191, 213, 717, 876, 1142
Sau3AI	GATC	8 cut(s)	104, 381, 392, 556, 628, 1050, 1395, 1645
Sau96I	GGNCC	9 cut(s)	131, 377, 428, 851, 1148, 1252, 1273, 1526, 1532
ScaI	AGTACT	1 cut(s)	97
SchI	GAGTC	2 cut(s)	215, 692
ScrFI	CCNGG	3 cut(s)	467, 1530, 1582
SduI	GDGCHC	1 cut(s)	156
SfaNI	GCATC	1 cut(s)	1231
SfcI	CTRYAG	2 cut(s)	717, 1798
SfuI	TTCGAA	1 cut(s)	479
SinI	GGWCC	6 cut(s)	377, 428, 1252, 1273, 1526, 1532
SmiMI	CAYNNNNRTG	2 cut(s)	969, 1391
SmlI	CTYRAG	1 cut(s)	91
SmoI	CTYRAG	1 cut(s)	91
SnaBI	TACGTA	1 cut(s)	544
SpeI	ACTAGT	2 cut(s)	704, 1100
SphI	GCATGC	1 cut(s)	1412
Sse9I	AATT	7 cut(s)	329, 607, 891, 1212, 1419, 1658, 1739
SsiI	CCGC	2 cut(s)	999, 1542
SspI	AATATT	2 cut(s)	808, 1321
SspMI	CTAG	2 cut(s)	705, 1101
StyD4I	CCNGG	3 cut(s)	465, 1528, 1580
StyI	CCWWGG	2 cut(s)	502, 1535
TaaI	ACNGT	8 cut(s)	184, 359, 388, 432, 603, 665, 1331, 1363
TaiI	ACGT	1 cut(s)	546
TaqI	TCGA	6 cut(s)	345, 391, 441, 479, 1482, 1648
TaqII	GACCGA	1 cut(s)	394
TasI	AATT	7 cut(s)	329, 607, 891, 1212, 1419, 1658, 1739
TatI	WGTACW	1 cut(s)	95
TfiI	GAWTC	4 cut(s)	527, 758, 1594, 1794
Tru1I	TTAA	5 cut(s)	335, 486, 1014, 1191, 1434
Tru9I	TTAA	5 cut(s)	335, 486, 1014, 1191, 1434
TscAI	CASTG	4 cut(s)	1342, 1393, 1448, 1635
TseFI	GTSAC	5 cut(s)	63, 143, 1019, 1331, 1363
TseI	GCWGC	5 cut(s)	190, 212, 716, 875, 1141
Tsp45I	GTSAC	5 cut(s)	63, 143, 1019, 1331, 1363
TspDTI	ATGAA	5 cut(s)	579, 1205, 1586, 1651, 1724
TspGWI	ACGGA	1 cut(s)	1509
TspRI	CASTG	4 cut(s)	1342, 1393, 1448, 1635
VpaK11BI	GGWCC	6 cut(s)	377, 428, 1252, 1273, 1526, 1532
XapI	RAATTY	3 cut(s)	329, 891, 1212
XceI	RCATGY	2 cut(s)	511, 1412
XcmI	CCANNNNNNNNNTGG	1 cut(s)	509
XmiI	GTMKAC	1 cut(s)	942
XmnI	GAANNNNTTC	1 cut(s)	895
XspI	CTAG	2 cut(s)	705, 1101
ZrmI	AGTACT	1 cut(s)	97
Zsp2I	ATGCAT	1 cut(s)	13

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.