pycom15g02340

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016040
Physical Location & Seq
Reverse (-)
1376456 .. 1377890
1435 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 915 bp
ATGCCATATGTTCTCCATTCCTTTCTATTAGTTTCCAAAATTACGTGCAGTTTGAAGGCGTTTCCGTGCAATTCAAAGACCATGTCGAGTCAAGACAATGGCTCGCCTGAGTTCCCAACGTGGCCGGAGTTCAAGCTTCCGGACTTGTTGTCCACAGACACCATTCGACAGGTTCATGCAACGATTGAGAATGAATGGGATCCTCTTCAGCGGTCGGCTTGCCAGACGGCAGCTGGGAGAGCCTTGTGGAATCATGTCATTCATGACCCCCTGGCAGACATACTTGCGGGAGAGTCCTACCTTAAAAGTCTCCATGAGAAGATAAAGAAAGACTGCGTCAACAAGGCGCGAGAAATCTCTGGTGTCATTCTTGCAGTTCGAACGCTCTGGTTTGATTCGAAGCTTGAAGCAGCACTTCATTCCTTCAACGACGGGGAAGCACAAGTAGTTCTTCTTGGGGCAGGAATGGATGCAAGGGCTTACCGATTGAGTTGCCTGAAGGAAAGCAATGTGTTTGAAGTTGATTTTCCTGATCTCCTGCAAATAAAAGCCACTCTCCTGCAAGCAGCGATGGATTCTACAAACGGTCACCCGTGTCTAATGATGACAGCAAAATCCATTACAAGAGTGGCGGCTGATATCAGAAGCAATGACTGGCTCGAAAAACTTCAAACATCAGGGTTTGTGCCAGAGAAGAACACAGTGTGGATGCTAGAAGGCATCCTCTACTACCTAACCCATTCCGAGGCCACGCAGGTACTTGAAACCATAGCAGGCAAGTGCTCGCTTACTCAGACGGTACTCTTGGCAGATTTTATGAACAAACCATCAATCAGTCTTTCCAATTCCATCTTCCATTTCTATAGCGATTGGCCTGATCGATCTCCTGCCATCTCTTGGCTTTTCTCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LCM

Protein Analysis

305

Amino Acids

33.99

Weight (kDa)

5.71

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 73 - 258 2.3e-43 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016040)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42760 AT5G42760
fragaria_vesca FvH4_2g39790
malus_domestica MD15G1027400.v1.1
prunus_persica Prupe.1G379200_v2.0.a1
pyrus_communis pycom15g02340
rosa_laevigata RLG00000011280
rosa_multiflora Rmu_sc0003168.1_g000012 Rmu_sc0035551.1_g000003
rosa_roxburghii Rroxscaffold_7G00167210
rosa_rugosa Rorug06G0300600
rosa_samantha Rh6AG414400 Rh6BG421300 Rh6CG428900 Rh6DG414300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 745
AccB7I CCANNNNNTGG 1 cut(s) 897
AccII CGCG 1 cut(s) 349
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 3 cut(s) 211, 287, 632
AclWI GGATC 2 cut(s) 194, 207
AcoI YGGCCR 1 cut(s) 122
AcuI CTGAAG 2 cut(s) 191, 518
AdeI CACNNNGTG 1 cut(s) 705
AfaI GTAC 2 cut(s) 759, 801
AfiI CCNNNNNNNGG 2 cut(s) 745, 897
AgsI TTSAA 8 cut(s) 55, 75, 133, 407, 427, 518, 671, 764
AhdI GACNNNNNGTC 1 cut(s) 148
AjnI CCWGG 1 cut(s) 270
AjuI GAANNNNNNNTTGG 2 cut(s) 836, 868
AluBI AGCT 3 cut(s) 136, 233, 403
AluI AGCT 3 cut(s) 136, 233, 403
Alw21I GWGCWC 1 cut(s) 785
Alw26I GTCTC 1 cut(s) 314
AlwI GGATC 2 cut(s) 194, 207
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 3 cut(s) 122, 747, 872
ApeKI GCWGC 3 cut(s) 230, 410, 566
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 1 cut(s) 666
AspLEI GCGC 1 cut(s) 349
AsuHPI GGTGA 1 cut(s) 581
AsuII TTCGAA 2 cut(s) 379, 398
BamHI GGATCC 1 cut(s) 199
Bbv12I GWGCWC 1 cut(s) 785
BbvI GCAGC 3 cut(s) 242, 422, 578
BccI CCATC 4 cut(s) 565, 835, 857, 899
BceAI ACGGC 1 cut(s) 243
BcgI CGANNNNNNTGC 2 cut(s) 474, 508
BciT130I CCWGG 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 314
BfaI CTAG 1 cut(s) 713
BfmI CTRYAG 1 cut(s) 862
BfuAI ACCTGC 1 cut(s) 745
BisI GCNGC 4 cut(s) 231, 411, 567, 633
BlsI GCNGC 4 cut(s) 232, 412, 568, 634
Bme1390I CCNGG 1 cut(s) 272
BmeRI GACNNNNNGTC 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 1 cut(s) 272
BmsI GCATC 3 cut(s) 460, 699, 729
Bpu14I TTCGAA 2 cut(s) 379, 398
Bsa29I ATCGAT 1 cut(s) 880
BsaAI YACGTR 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 270, 744
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 2 cut(s) 745, 897
Bse1I ACTGG 1 cut(s) 659
Bse3DI GCAATG 2 cut(s) 514, 655
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 1 cut(s) 272
BseCI ATCGAT 1 cut(s) 880
BseDI CCNNGG 2 cut(s) 270, 744
BseGI GGATG 3 cut(s) 475, 714, 720
BseLI CCNNNNNNNGG 2 cut(s) 745, 897
BseMI GCAATG 2 cut(s) 514, 655
BseMII CTCAG 2 cut(s) 99, 806
BseNI ACTGG 1 cut(s) 659
BseXI GCAGC 3 cut(s) 242, 422, 578
BseYI CCCAGC 1 cut(s) 233
BsgI GTGCAG 1 cut(s) 67
Bsh1236I CGCG 1 cut(s) 349
Bsh1285I CGRYCG 1 cut(s) 215
BshFI GGCC 3 cut(s) 124, 749, 874
BshVI ATCGAT 1 cut(s) 880
BsiEI CGRYCG 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 785
BsiSI CCGG 2 cut(s) 125, 140
BslI CCNNNNNNNGG 2 cut(s) 745, 897
BsmAI GTCTC 1 cut(s) 314
BsnI GGCC 3 cut(s) 124, 749, 874
Bsp119I TTCGAA 2 cut(s) 379, 398
Bsp1286I GDGCHC 1 cut(s) 785
Bsp13I TCCGGA 1 cut(s) 139
Bsp143I GATC 4 cut(s) 199, 532, 877, 881
BspACI CCGC 3 cut(s) 211, 287, 632
BspANI GGCC 3 cut(s) 124, 749, 874
BspCNI CTCAG 2 cut(s) 100, 805
BspDI ATCGAT 1 cut(s) 880
BspEI TCCGGA 1 cut(s) 139
BspFNI CGCG 1 cut(s) 349
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 201
BspMI ACCTGC 1 cut(s) 745
BspPI GGATC 2 cut(s) 194, 207
BspT104I TTCGAA 2 cut(s) 379, 398
BsrDI GCAATG 2 cut(s) 514, 655
BsrI ACTGG 1 cut(s) 659
BssECI CCNNGG 2 cut(s) 270, 744
BssMI GATC 4 cut(s) 199, 532, 877, 881
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 3 cut(s) 587, 703, 799
Bst6I CTCTTC 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 45
BstBI TTCGAA 2 cut(s) 379, 398
BstC8I GCNNGC 5 cut(s) 104, 220, 564, 775, 785
BstDEI CTNAG 2 cut(s) 108, 792
BstEII GGTNACC 1 cut(s) 587
BstF5I GGATG 3 cut(s) 475, 714, 720
BstFNI CGCG 1 cut(s) 349
BstHHI GCGC 1 cut(s) 349
BstKTI GATC 4 cut(s) 202, 535, 880, 884
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 4 cut(s) 199, 532, 877, 881
BstMCI CGRYCG 1 cut(s) 215
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 272
BstPI GGTNACC 1 cut(s) 587
BstSCI CCNGG 1 cut(s) 270
BstSFI CTRYAG 1 cut(s) 862
BstUI CGCG 1 cut(s) 349
BstV1I GCAGC 3 cut(s) 242, 422, 578
BstX2I RGATCY 1 cut(s) 199
BstYI RGATCY 1 cut(s) 199
Bsu15I ATCGAT 1 cut(s) 880
BsuRI GGCC 3 cut(s) 124, 749, 874
BsuTUI ATCGAT 1 cut(s) 880
BtgZI GCGATG 1 cut(s) 584
BtsCI GGATG 3 cut(s) 475, 714, 720
BtsIMutI CAGTG 1 cut(s) 708
BveI ACCTGC 1 cut(s) 745
Cac8I GCNNGC 5 cut(s) 104, 220, 564, 775, 785
CciI TCATGA 1 cut(s) 262
CfoI GCGC 1 cut(s) 349
ClaI ATCGAT 1 cut(s) 880
CseI GACGC 1 cut(s) 325
Csp6I GTAC 2 cut(s) 758, 800
CviAII CATG 6 cut(s) 82, 176, 254, 263, 314, 909
CviQI GTAC 2 cut(s) 758, 800
DdeI CTNAG 2 cut(s) 108, 792
DpnI GATC 4 cut(s) 201, 534, 879, 883
DpnII GATC 4 cut(s) 199, 532, 877, 881
DraIII CACNNNGTG 1 cut(s) 705
DriI GACNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 122
Eam1104I CTCTTC 1 cut(s) 210
Eam1105I GACNNNNNGTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 210
Eco32I GATATC 1 cut(s) 640
Eco57I CTGAAG 2 cut(s) 191, 518
Eco91I GGTNACC 1 cut(s) 587
EcoO65I GGTNACC 1 cut(s) 587
EcoRII CCWGG 1 cut(s) 270
EcoRV GATATC 1 cut(s) 640
FaeI CATG 6 cut(s) 85, 179, 257, 266, 317, 912
FalI AAGNNNNNCTT 4 cut(s) 399, 431, 435, 467
FatI CATG 6 cut(s) 81, 175, 253, 262, 313, 908
FauI CCCGC 1 cut(s) 280
FauNDI CATATG 1 cut(s) 7
Fnu4HI GCNGC 4 cut(s) 231, 411, 567, 633
FokI GGATG 3 cut(s) 482, 707, 721
Fsp4HI GCNGC 4 cut(s) 231, 411, 567, 633
FspBI CTAG 1 cut(s) 713
GlaI GCGC 1 cut(s) 348
GluI GCNGC 4 cut(s) 231, 411, 567, 633
GsaI CCCAGC 1 cut(s) 237
HaeIII GGCC 3 cut(s) 124, 749, 874
HapII CCGG 2 cut(s) 125, 140
HgaI GACGC 1 cut(s) 325
HhaI GCGC 1 cut(s) 349
Hin1II CATG 6 cut(s) 85, 179, 257, 266, 317, 912
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 2 cut(s) 134, 401
HinfI GANTC 5 cut(s) 88, 250, 293, 395, 575
HpaII CCGG 2 cut(s) 125, 140
HphI GGTGA 1 cut(s) 581
Hpy166II GTNNAC 2 cut(s) 153, 340
Hpy188I TCNGA 3 cut(s) 644, 745, 795
Hpy188III TCNNGA 4 cut(s) 92, 140, 263, 530
Hpy8I GTNNAC 2 cut(s) 153, 340
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 4 cut(s) 49, 433, 493, 710
HpyCH4III ACNGT 3 cut(s) 587, 703, 799
HpyCH4IV ACGT 2 cut(s) 44, 119
HpyCH4V TGCA 7 cut(s) 48, 69, 179, 374, 473, 541, 562
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 2 cut(s) 108, 792
HpySE526I ACGT 2 cut(s) 44, 119
Hsp92II CATG 6 cut(s) 85, 179, 257, 266, 317, 912
HspAI GCGC 1 cut(s) 347
Kpn2I TCCGGA 1 cut(s) 139
Kzo9I GATC 4 cut(s) 199, 532, 877, 881
Lsp1109I GCAGC 3 cut(s) 242, 422, 578
LweI GCATC 3 cut(s) 460, 699, 729
MaeI CTAG 1 cut(s) 713
MaeII ACGT 2 cut(s) 44, 119
MaeIII GTNAC 1 cut(s) 587
MalI GATC 4 cut(s) 201, 534, 879, 883
MboI GATC 4 cut(s) 199, 532, 877, 881
MboII GAAGA 5 cut(s) 197, 331, 443, 706, 844
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 785
MluCI AATT 3 cut(s) 39, 70, 844
MlyI GAGTC 2 cut(s) 97, 302
MnlI CCTC 3 cut(s) 213, 734, 739
MroI TCCGGA 1 cut(s) 139
MroXI GAANNNNTTC 1 cut(s) 666
MseI TTAA 2 cut(s) 303, 913
MspA1I CMGCKG 2 cut(s) 211, 233
MspI CCGG 2 cut(s) 125, 140
MspR9I CCNGG 1 cut(s) 272
MvaI CCWGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 1 cut(s) 239
NdeI CATATG 1 cut(s) 7
NdeII GATC 4 cut(s) 199, 532, 877, 881
NlaIII CATG 6 cut(s) 85, 179, 257, 266, 317, 912
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 587
NspV TTCGAA 2 cut(s) 379, 398
PagI TCATGA 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 666
PfeI GAWTC 3 cut(s) 250, 395, 575
PflFI GACNNNGTC 2 cut(s) 82, 335
PflMI CCANNNNNTGG 1 cut(s) 897
PkrI GCNGC 4 cut(s) 232, 412, 568, 634
PleI GAGTC 2 cut(s) 96, 301
PpsI GAGTC 2 cut(s) 96, 301
Ppu21I YACGTR 1 cut(s) 45
Psp6I CCWGG 1 cut(s) 270
PspEI GGTNACC 1 cut(s) 587
PspFI CCCAGC 1 cut(s) 233
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 1 cut(s) 201
PsuI RGATCY 1 cut(s) 199
PsyI GACNNNGTC 2 cut(s) 82, 335
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 2 cut(s) 759, 801
RsaNI GTAC 2 cut(s) 758, 800
SaqAI TTAA 2 cut(s) 303, 913
SatI GCNGC 4 cut(s) 231, 411, 567, 633
Sau3AI GATC 4 cut(s) 199, 532, 877, 881
SchI GAGTC 2 cut(s) 97, 302
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 1 cut(s) 785
SetI ASST 9 cut(s) 47, 122, 138, 174, 235, 303, 405, 735, 759
SfaNI GCATC 3 cut(s) 460, 699, 729
SfcI CTRYAG 1 cut(s) 862
SfuI TTCGAA 2 cut(s) 379, 398
Sse9I AATT 3 cut(s) 39, 70, 844
SsiI CCGC 3 cut(s) 211, 287, 632
SspMI CTAG 1 cut(s) 713
StyD4I CCNGG 1 cut(s) 270
TaaI ACNGT 3 cut(s) 587, 703, 799
TaiI ACGT 2 cut(s) 47, 122
TaqI TCGA 6 cut(s) 86, 166, 379, 398, 660, 880
TasI AATT 3 cut(s) 39, 70, 844
TauI GCSGC 1 cut(s) 635
TfiI GAWTC 3 cut(s) 250, 395, 575
Tru1I TTAA 2 cut(s) 303, 913
Tru9I TTAA 2 cut(s) 303, 913
TscAI CASTG 1 cut(s) 708
TseFI GTSAC 1 cut(s) 587
TseI GCWGC 3 cut(s) 230, 410, 566
Tsp45I GTSAC 1 cut(s) 587
TspDTI ATGAA 5 cut(s) 164, 207, 251, 407, 833
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 1 cut(s) 708
Tth111I GACNNNGTC 2 cut(s) 82, 335
Van91I CCANNNNNTGG 1 cut(s) 897
XcmI CCANNNNNNNNNTGG 2 cut(s) 230, 625
XmnI GAANNNNTTC 1 cut(s) 666
XspI CTAG 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.