pycom15g02720

Glucose-6-phosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006883
Physical Location & Seq
Reverse (-)
1663249 .. 1666519
3271 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1047 bp
ATGTTGAATGCTCGAACCCTTAGGGAATGGATTTCATCTTCACTCGGGCCTGCTGCAGTTGCAAAGCATATGGTTGCAGTCAGCACTAATCTTACGCTTGTAGAGAAATTTGGAATTGACCCAAGAAATGCTTTTGCATTTTGGGACTGGGTTGGTGGCCGATATAGTGTTTGTAGTGCTGTTGGAGTGTTGCCCTTATCTCTTCAGTATGGGTTCTCAGTTGTTGAGAAGTTCTTAAAGGGAGCTTCAAGCATCGATCAACACTTCTACTCAACACCATTTGAGAAAAATATACCTGTTCTTTTAGGTCTGTTGAGCATATGGAATGTATCATTTCTTGGATACCCTGCAAGAGCTATCTTACCTTACTCCCAAGCACTGGAGAAGCTTGCTCCGCACATTCAACAGGTTAGCATGGAAAGTAACGGCAAGGGTGTATCAATTGATGGTGTAATACTTCCCTTTGAGACTGGTGAAATAGACTTTGGTGAACCTGGAACAAATGGTCAGCACAGCTTTTACCAACTAATTCACCAGGGCCGTGTTATTCCTTGTGACTTTATTGGTGTTGTGAAGAGTCAGCAACCTGTTTACCTGAAAGGTGAAGTGGTGAGTAATCATGATGAGCTCATGTCTAACTTTTTTGCACAGCCCGATGCCCTTGCCTATGGAAAGCACCCGGAACAGCTGAAGAAAGAGAATGTTCCAGAGCATCTTATCACTCATAAGACTTTCACTGGAAATCGGCCTTCTCTCAGCCTTCTGCTTCCATCACTTAATGCTTACAACATCGGGCAGTTGTTGGCAATTTATGAACACAGAATTGCAGTTGAAGGTTTTGTATGGGGAATCAATTCTTTTGACCAGTGGGGAGTAGAATTGGGAAAGTCATTGGCTAGTCAAGTTAGAAAGCAACTCAATGCATCTCGTACAAAAGGGCAACCTATCGAGGGCTTCAACTACAGTACCGCAACGTTGTTAACAAAATACTTGGAGGCAAGTAAAGATATTCCTGCTGACCTTCCGACACTTTTGCCACAGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004347 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00500 ko00520 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00030 map00500 map00520 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
PGI

Protein Analysis

349

Amino Acids

38.42

Weight (kDa)

6.56

Isoelectric Point (pI)

32.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGI PF00342 1 - 327 6.3e-145 Phosphoglucose isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 379
AciI CCGC 2 cut(s) 395, 969
AclI AACGTT 1 cut(s) 974
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 1 cut(s) 107
AcuI CTGAAG 2 cut(s) 188, 710
AfaI GTAC 2 cut(s) 931, 967
AfiI CCNNNNNNNGG 2 cut(s) 379, 950
AgsI TTSAA 5 cut(s) 7, 249, 404, 833, 958
AjnI CCWGG 2 cut(s) 493, 534
AjuI GAANNNNNNNTTGG 2 cut(s) 468, 500
AluBI AGCT 6 cut(s) 245, 356, 388, 516, 628, 688
AluI AGCT 6 cut(s) 245, 356, 388, 516, 628, 688
Alw21I GWGCWC 1 cut(s) 630
Alw26I GTCTC 1 cut(s) 461
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 4 cut(s) 47, 157, 538, 746
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 1 cut(s) 107
Asp700I GAANNNNTTC 1 cut(s) 853
AspS9I GGNCC 2 cut(s) 47, 538
AsuC2I CCSGG 1 cut(s) 680
AsuHPI GGTGA 5 cut(s) 485, 500, 524, 614, 622
AvaI CYCGRG 1 cut(s) 44
AxyI CCTNAGG 1 cut(s) 20
BaeI ACNNNNGTAYC 2 cut(s) 949, 982
BanII GRGCYC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 630
BbvI GCAGC 1 cut(s) 40
BccI CCATC 2 cut(s) 440, 778
BceAI ACGGC 2 cut(s) 442, 525
BcgI CGANNNNNNTGC 3 cut(s) 644, 678, 1015
BciT130I CCWGG 2 cut(s) 495, 536
BciVI GTATCC 1 cut(s) 335
BcnI CCSGG 1 cut(s) 680
BcoDI GTCTC 1 cut(s) 461
BfaI CTAG 1 cut(s) 897
BfmI CTRYAG 2 cut(s) 54, 961
BfuI GTATCC 1 cut(s) 335
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
Bme1390I CCNGG 3 cut(s) 495, 536, 680
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 47, 538
BmrFI CCNGG 3 cut(s) 495, 536, 680
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 4 cut(s) 261, 646, 721, 932
BmuI ACTGGG 1 cut(s) 157
BpmI CTGGAG 1 cut(s) 401
BpuMI CCSGG 1 cut(s) 680
Bsa29I ATCGAT 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 535
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 950
Bse1I ACTGG 5 cut(s) 152, 384, 475, 742, 865
Bse21I CCTNAGG 1 cut(s) 20
BseBI CCWGG 2 cut(s) 495, 536
BseCI ATCGAT 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 535
BseLI CCNNNNNNNGG 2 cut(s) 379, 950
BseMII CTCAG 2 cut(s) 231, 769
BseNI ACTGG 5 cut(s) 152, 384, 475, 742, 865
BseXI GCAGC 1 cut(s) 40
BshFI GGCC 4 cut(s) 49, 159, 540, 748
BshVI ATCGAT 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 630
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 1 cut(s) 680
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 2 cut(s) 379, 950
BsmAI GTCTC 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 158
BsmI GAATGC 1 cut(s) 13
BsnI GGCC 4 cut(s) 49, 159, 540, 748
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 630
Bsp143I GATC 1 cut(s) 256
BspACI CCGC 2 cut(s) 395, 969
BspANI GGCC 4 cut(s) 49, 159, 540, 748
BspCNI CTCAG 2 cut(s) 230, 768
BspDI ATCGAT 1 cut(s) 255
BspHI TCATGA 1 cut(s) 619
BspMAI CTGCAG 1 cut(s) 58
BsrI ACTGG 5 cut(s) 152, 384, 475, 742, 865
BssECI CCNNGG 1 cut(s) 535
BssMI GATC 1 cut(s) 256
Bst2UI CCWGG 2 cut(s) 495, 536
Bst4CI ACNGT 1 cut(s) 965
Bst6I CTCTTC 2 cut(s) 207, 569
BstC8I GCNNGC 2 cut(s) 51, 390
BstDEI CTNAG 3 cut(s) 20, 217, 755
BstKTI GATC 1 cut(s) 259
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 1 cut(s) 256
BstMWI GCNNNNNNNGC 2 cut(s) 59, 394
BstNI CCWGG 2 cut(s) 495, 536
BstSCI CCNGG 3 cut(s) 493, 534, 678
BstSFI CTRYAG 2 cut(s) 54, 961
BstV1I GCAGC 1 cut(s) 40
Bsu15I ATCGAT 1 cut(s) 255
Bsu36I CCTNAGG 1 cut(s) 20
BsuI GTATCC 1 cut(s) 335
BsuRI GGCC 4 cut(s) 49, 159, 540, 748
BsuTUI ATCGAT 1 cut(s) 255
BtsIMutI CAGTG 3 cut(s) 377, 735, 872
Cac8I GCNNGC 2 cut(s) 51, 390
CciI TCATGA 1 cut(s) 619
Cfr13I GGNCC 2 cut(s) 47, 538
ClaI ATCGAT 1 cut(s) 255
Csp6I GTAC 2 cut(s) 930, 966
CviAII CATG 3 cut(s) 415, 620, 631
CviQI GTAC 2 cut(s) 930, 966
DdeI CTNAG 3 cut(s) 20, 217, 755
DpnI GATC 1 cut(s) 258
DpnII GATC 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 2 cut(s) 207, 569
EarI CTCTTC 2 cut(s) 207, 569
Ecl136II GAGCTC 1 cut(s) 628
Eco24I GRGCYC 1 cut(s) 630
Eco53kI GAGCTC 1 cut(s) 628
Eco57I CTGAAG 2 cut(s) 188, 710
Eco81I CCTNAGG 1 cut(s) 20
Eco88I CYCGRG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 628
EcoRII CCWGG 2 cut(s) 493, 534
EcoT22I ATGCAT 1 cut(s) 925
EcoT38I GRGCYC 1 cut(s) 630
FaeI CATG 3 cut(s) 418, 623, 634
FalI AAGNNNNNCTT 2 cut(s) 115, 147
FaqI GGGAC 1 cut(s) 158
FatI CATG 3 cut(s) 414, 619, 630
FauNDI CATATG 2 cut(s) 69, 320
Fnu4HI GCNGC 1 cut(s) 54
FriOI GRGCYC 1 cut(s) 630
Fsp4HI GCNGC 1 cut(s) 54
FspBI CTAG 1 cut(s) 897
GluI GCNGC 1 cut(s) 54
GsuI CTGGAG 1 cut(s) 401
HaeIII GGCC 4 cut(s) 49, 159, 540, 748
HapII CCGG 1 cut(s) 680
Hin1II CATG 3 cut(s) 418, 623, 634
HincII GTYRAC 1 cut(s) 981
HindII GTYRAC 1 cut(s) 981
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 2 cut(s) 577, 849
HpaI GTTAAC 1 cut(s) 981
HpaII CCGG 1 cut(s) 680
HphI GGTGA 5 cut(s) 485, 500, 524, 614, 622
Hpy166II GTNNAC 3 cut(s) 491, 592, 981
Hpy188I TCNGA 1 cut(s) 1026
Hpy188III TCNNGA 2 cut(s) 620, 707
Hpy8I GTNNAC 3 cut(s) 491, 592, 981
HpyAV CCTTC 4 cut(s) 759, 770, 827, 1031
HpyCH4III ACNGT 1 cut(s) 965
HpyCH4IV ACGT 1 cut(s) 974
HpyCH4V TGCA 8 cut(s) 56, 62, 77, 137, 350, 647, 827, 923
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 394
HpyF3I CTNAG 3 cut(s) 20, 217, 755
HpySE526I ACGT 1 cut(s) 974
Hsp92II CATG 3 cut(s) 418, 623, 634
KspAI GTTAAC 1 cut(s) 981
Kzo9I GATC 1 cut(s) 256
LmnI GCTCC 2 cut(s) 242, 397
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 4 cut(s) 261, 646, 721, 932
MaeI CTAG 1 cut(s) 897
MaeII ACGT 1 cut(s) 974
MaeIII GTNAC 2 cut(s) 422, 554
MalI GATC 1 cut(s) 258
MboI GATC 1 cut(s) 256
MboII GAAGA 4 cut(s) 30, 194, 586, 703
MfeI CAATTG 1 cut(s) 441
MhlI GDGCHC 1 cut(s) 630
MluCI AATT 8 cut(s) 107, 114, 441, 528, 807, 822, 853, 878
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 2 cut(s) 943, 988
Mph1103I ATGCAT 1 cut(s) 925
MroXI GAANNNNTTC 1 cut(s) 853
MseI TTAA 3 cut(s) 236, 777, 980
MspA1I CMGCKG 1 cut(s) 688
MspI CCGG 1 cut(s) 680
MspR9I CCNGG 3 cut(s) 495, 536, 680
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 13
MvaI CCWGG 2 cut(s) 495, 536
MwoI GCNNNNNNNGC 2 cut(s) 59, 394
NciI CCSGG 1 cut(s) 680
NdeI CATATG 2 cut(s) 69, 320
NdeII GATC 1 cut(s) 256
NlaIII CATG 3 cut(s) 418, 623, 634
NmuCI GTSAC 1 cut(s) 554
NsiI ATGCAT 1 cut(s) 925
PagI TCATGA 1 cut(s) 619
PctI GAATGC 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 853
PfeI GAWTC 1 cut(s) 849
PflMI CCANNNNNTGG 1 cut(s) 379
PkrI GCNGC 1 cut(s) 55
PleI GAGTC 1 cut(s) 585
PpsI GAGTC 1 cut(s) 585
Psp124BI GAGCTC 1 cut(s) 630
Psp1406I AACGTT 1 cut(s) 974
Psp6I CCWGG 2 cut(s) 493, 534
PspGI CCWGG 2 cut(s) 493, 534
PspPI GGNCC 2 cut(s) 47, 538
PstI CTGCAG 1 cut(s) 58
PvuII CAGCTG 1 cut(s) 688
RsaI GTAC 2 cut(s) 931, 967
RsaNI GTAC 2 cut(s) 930, 966
SacI GAGCTC 1 cut(s) 630
SaqAI TTAA 3 cut(s) 236, 777, 980
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 1 cut(s) 256
Sau96I GGNCC 2 cut(s) 47, 538
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 3 cut(s) 495, 536, 680
SduI GDGCHC 1 cut(s) 630
SfaNI GCATC 4 cut(s) 261, 646, 721, 932
SfcI CTRYAG 2 cut(s) 54, 961
Sse9I AATT 8 cut(s) 107, 114, 441, 528, 807, 822, 853, 878
SsiI CCGC 2 cut(s) 395, 969
SspMI CTAG 1 cut(s) 897
SstI GAGCTC 1 cut(s) 630
StyD4I CCNGG 3 cut(s) 493, 534, 678
TaaI ACNGT 1 cut(s) 965
TaiI ACGT 1 cut(s) 977
TaqI TCGA 3 cut(s) 13, 255, 948
TasI AATT 8 cut(s) 107, 114, 441, 528, 807, 822, 853, 878
TfiI GAWTC 1 cut(s) 849
Tru1I TTAA 3 cut(s) 236, 777, 980
Tru9I TTAA 3 cut(s) 236, 777, 980
TscAI CASTG 3 cut(s) 384, 742, 872
TseFI GTSAC 1 cut(s) 554
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 554
TspDTI ATGAA 2 cut(s) 24, 828
TspRI CASTG 3 cut(s) 384, 742, 872
Van91I CCANNNNNTGG 1 cut(s) 379
XapI RAATTY 1 cut(s) 107
XmnI GAANNNNTTC 1 cut(s) 853
XspI CTAG 1 cut(s) 897
Zsp2I ATGCAT 1 cut(s) 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.