pycom15g12660

MACPF domain-containing protein At4g24290-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015525
Physical Location & Seq
Forward (+)
8660092 .. 8664139
4048 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1587 bp
ATGCGGTTCGCCTCTGATGTCCTCTCCTTCCAACAGATGTCGGAGCAATTCAACCAGGAAGTGGGTGTGTCAGGTAAAATACCAACAGGGCAGTTCAATACAGCTTTTGAGTTTACAGGAGGATGGCAAAAGGACGCTGCCAACACCAAAACACTTGCTTTTGATGGAGTCTCCATCACCCTCTACAGCGTTGCTCTCGATAAATCCCAGGTTGCCTTGTGTGACCATATCAAACACGCTGTCCCCTCCTCCTGGGATCCCGCCGCACTTGCTAGGTTTATTGACAAGTATGGCACACATGTCATCGTTGGGATCAAAATGGGTGGAAAGGATACCATATATGCCAAGCAGCAGCACTCCTCGCCTCTCCAACCTGCTGATGTTCAGAAAAAATTGAAGGACATGGCTGATAAAATGTATATAGATGCAACTGCTCACTCTAGTCTCACTTCCGCAAAGTTGAATGAGAGAGACAAGTTAGTCAAGGAGTCAAGGGCTATCATGGATAATATCCCATCCAGTTCTTATGCTACTGTAGAGTTTCAGGATATTAAATTCATGTGCAAGCGGAAGGGTGGAAGCTTGTATAAAAATCTGCCTCACAATGATTGGTGTCAAACAGTTCAGACTGAACCTGATGTGATCTCTATGTCATTAGTTCCTATCCAATCCTTACTGAGTGGGATACATGGGAGTGGATTTTTGAGCCATGCTATTAATCTCTATCTTCGTTATAAACCGCCAATTGAAGAGCTGCATCAATTTTTGGAGTTTCAACTTCCAAGGCAATGGGCACCAGTGTTTGGTGAGCTTGCTCTTGGTCCTCAGAGGCAGCAGCAAAGCGGTGCATCTTTACAGTTTAGCTTCATGGGTCCTAAGCTTTATGTTAACAAAAATCCGGTTGATGTGGGTAAGAAGCCGGTGACTGGCCTCCGGTTGTATTTGGAGGGAAAAAGAAGCAACTGCTTGGCAATTCACCTGCAGCATCTCTCTACGCTGCCAAAGACCTTCCAGCTCCAGGATGCAGTGAATGGAAATATCTCTCGACTTTCATCAGACCGTAGATACTTCGAAAAGGTTCAATGGAAGAGCTTCTCTCATGTCTGCACAGCTCCTGTTGAGGCTAATGATGATTTGTCCGTTGTCACTGGCGCTCACTTTGAAGTTGGAGACTCTGGCCTGAAGAAAGTTTTATTTTTACGGCTTCATTTTTCTAAAGTAATAGGTGCGACTAATGTTAGACAGGCTGAGTGGGATGGTTCCCCTGGCTTGGCGCAGAAATCTGGATTAATTTCAACCCTTATTAGCACCCGTTTCTCGAGTGCTAAGAAGCCACCTCCTCAGCCTGCTGATGTGAACATAAACTCGGCTGTGTATCCTGGGGGCCCTCCAGTGCCAACACAGACACCTAAACTATTGAGGTTTGTGGATACGACCGAAATGACAAGAGGACCGCAGGATTCACCTGGGTATTGGGTTGTGTCGGGGGCAAGGCTTATCGCTGAGAATGGTAAAATATCTCTACGAGTCAAGTATTCTCTGCTGACCATGATTTTGCCTGATGAAGAGATGCAAGATAACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

529

Amino Acids

58.49

Weight (kDa)

9.05

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 31 - 242 1.9e-36 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015525)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25760 FvH4_1g25760
malus_domestica MD15G1140100.v1.1
prunus_persica Prupe.4G285900_v2.0.a1
pyrus_communis pycom15g12660
rosa_chinensis RchiOBHm_Chr3g0484761
rosa_laevigata RLG00000023205
rosa_multiflora Rmu_sc0001689.1_g000010 Rmu_sc0006653.1_g000005
rosa_roxburghii Rroxscaffold_6G00397460
rosa_rugosa Rorug03G0213800
rosa_samantha Rh3AG264100 Rh3BG299100 Rh3CG296900 Rh3DG292700
rosa_wichuraiana Rw3G023550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 735
AarI CACCTGC 1 cut(s) 987
AasI GACNNNNNNGTC 1 cut(s) 479
Acc36I ACCTGC 2 cut(s) 382, 987
AccB1I GGYRCC 1 cut(s) 793
AccB7I CCANNNNNTGG 2 cut(s) 61, 803
AciI CCGC 8 cut(s) 4, 261, 264, 453, 568, 740, 843, 1454
AclWI GGATC 3 cut(s) 251, 264, 320
AcsI RAATTY 1 cut(s) 554
AcuI CTGAAG 1 cut(s) 1202
AfiI CCNNNNNNNGG 6 cut(s) 61, 252, 803, 926, 1018, 1270
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 9 cut(s) 52, 97, 397, 463, 749, 776, 1082, 1163, 1296
AjnI CCWGG 7 cut(s) 54, 207, 251, 1017, 1264, 1378, 1465
AluBI AGCT 9 cut(s) 104, 582, 754, 811, 864, 880, 1015, 1092, 1112
AluI AGCT 9 cut(s) 104, 582, 754, 811, 864, 880, 1015, 1092, 1112
Alw26I GTCTC 4 cut(s) 175, 449, 465, 1164
AlwI GGATC 3 cut(s) 251, 264, 320
AlwNI CAGNNNCTG 1 cut(s) 1115
Ama87I CYCGRG 1 cut(s) 1318
AoxI GGCC 3 cut(s) 928, 1177, 1384
ApaI GGGCCC 1 cut(s) 1388
ApeKI GCWGC 8 cut(s) 137, 349, 352, 754, 832, 835, 982, 997
ApoI RAATTY 1 cut(s) 554
ArsI GACNNNNNNTTYG 4 cut(s) 225, 257, 1537, 1569
AseI ATTAAT 2 cut(s) 717, 1289
Asp700I GAANNNNTTC 2 cut(s) 1077, 1091
AspLEI GCGC 2 cut(s) 1154, 1276
AspS9I GGNCC 5 cut(s) 821, 872, 1384, 1385, 1451
AsuHPI GGTGA 5 cut(s) 169, 818, 934, 968, 1455
AsuII TTCGAA 1 cut(s) 1071
AvaI CYCGRG 1 cut(s) 1318
AvaII GGWCC 3 cut(s) 821, 872, 1451
BaeGI GKGCMC 2 cut(s) 796, 1388
BamHI GGATCC 1 cut(s) 256
BanI GGYRCC 1 cut(s) 793
BanII GRGCYC 1 cut(s) 1388
BbvCI CCTCAGC 1 cut(s) 1341
BbvI GCAGC 8 cut(s) 124, 361, 364, 741, 844, 847, 984, 994
BccI CCATC 5 cut(s) 117, 158, 182, 523, 1250
BceAI ACGGC 1 cut(s) 1217
BciT130I CCWGG 7 cut(s) 56, 209, 253, 1019, 1266, 1380, 1467
BciVI GTATCC 4 cut(s) 325, 678, 1386, 1423
BcoDI GTCTC 4 cut(s) 175, 449, 465, 1164
BfaI CTAG 2 cut(s) 273, 441
BfmI CTRYAG 3 cut(s) 184, 534, 980
BfoI RGCGCY 1 cut(s) 1155
BfuAI ACCTGC 2 cut(s) 382, 987
BfuI GTATCC 4 cut(s) 325, 678, 1386, 1423
BisI GCNGC 9 cut(s) 138, 264, 350, 353, 755, 833, 836, 983, 998
BlsI GCNGC 9 cut(s) 139, 265, 351, 354, 756, 834, 837, 984, 999
Bme1390I CCNGG 7 cut(s) 56, 209, 253, 1019, 1266, 1380, 1467
Bme18I GGWCC 3 cut(s) 821, 872, 1451
BmeT110I CYCGRG 1 cut(s) 1318
BmgT120I GGNCC 5 cut(s) 821, 872, 1384, 1385, 1451
BmiI GGNNCC 6 cut(s) 258, 795, 873, 1261, 1385, 1386
BmrFI CCNGG 7 cut(s) 56, 209, 253, 1019, 1266, 1380, 1467
BmsI GCATC 6 cut(s) 415, 766, 857, 994, 1012, 1560
BplI GAGNNNNNCTC 2 cut(s) 1081, 1113
BpmI CTGGAG 2 cut(s) 1001, 1374
Bpu10I CCTNAGC 2 cut(s) 876, 1341
Bpu14I TTCGAA 1 cut(s) 1071
BsaJI CCNNGG 6 cut(s) 207, 252, 782, 1264, 1379, 1466
BsaWI WCCGGW 2 cut(s) 898, 933
Bsc4I CCNNNNNNNGG 6 cut(s) 61, 252, 803, 926, 1018, 1270
Bse118I RCCGGY 1 cut(s) 919
Bse1I ACTGG 5 cut(s) 519, 797, 931, 1153, 1391
Bse3DI GCAATG 1 cut(s) 794
BseBI CCWGG 7 cut(s) 56, 209, 253, 1019, 1266, 1380, 1467
BseDI CCNNGG 6 cut(s) 207, 252, 782, 1264, 1379, 1466
BseGI GGATG 4 cut(s) 128, 515, 1027, 1261
BseLI CCNNNNNNNGG 6 cut(s) 61, 252, 803, 926, 1018, 1270
BseMI GCAATG 1 cut(s) 794
BseMII CTCAG 5 cut(s) 668, 839, 1239, 1355, 1494
BseNI ACTGG 5 cut(s) 519, 797, 931, 1153, 1391
BseRI GAGGAG 3 cut(s) 238, 349, 1329
BseSI GKGCMC 2 cut(s) 796, 1388
BseXI GCAGC 8 cut(s) 124, 361, 364, 741, 844, 847, 984, 994
BsgI GTGCAG 1 cut(s) 1090
Bsh1285I CGRYCG 1 cut(s) 1437
BshFI GGCC 3 cut(s) 930, 1179, 1386
BshNI GGYRCC 1 cut(s) 793
BsiEI CGRYCG 1 cut(s) 1437
BsiHKCI CYCGRG 1 cut(s) 1318
BsiSI CCGG 3 cut(s) 899, 920, 934
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 6 cut(s) 61, 252, 803, 926, 1018, 1270
BsmAI GTCTC 4 cut(s) 175, 449, 465, 1164
BsmFI GGGAC 1 cut(s) 227
BsnI GGCC 3 cut(s) 930, 1179, 1386
BsoBI CYCGRG 1 cut(s) 1318
Bsp119I TTCGAA 1 cut(s) 1071
Bsp120I GGGCCC 1 cut(s) 1384
Bsp1286I GDGCHC 2 cut(s) 796, 1388
Bsp143I GATC 3 cut(s) 256, 312, 642
BspACI CCGC 8 cut(s) 4, 261, 264, 453, 568, 740, 843, 1454
BspANI GGCC 3 cut(s) 930, 1179, 1386
BspCNI CTCAG 5 cut(s) 669, 838, 1240, 1354, 1495
BspLI GGNNCC 6 cut(s) 258, 795, 873, 1261, 1385, 1386
BspMAI CTGCAG 1 cut(s) 984
BspMI ACCTGC 2 cut(s) 382, 987
BspPI GGATC 3 cut(s) 251, 264, 320
BspQI GCTCTTC 2 cut(s) 744, 1082
BspT104I TTCGAA 1 cut(s) 1071
BspT107I GGYRCC 1 cut(s) 793
BsrDI GCAATG 1 cut(s) 794
BsrFI RCCGGY 1 cut(s) 919
BsrI ACTGG 5 cut(s) 519, 797, 931, 1153, 1391
BssAI RCCGGY 1 cut(s) 919
BssECI CCNNGG 6 cut(s) 207, 252, 782, 1264, 1379, 1466
BssMI GATC 3 cut(s) 256, 312, 642
BssT1I CCWWGG 1 cut(s) 782
Bst2UI CCWGG 7 cut(s) 56, 209, 253, 1019, 1266, 1380, 1467
Bst4CI ACNGT 4 cut(s) 535, 622, 858, 1061
Bst6I CTCTTC 3 cut(s) 744, 1082, 1560
BstBI TTCGAA 1 cut(s) 1071
BstC8I GCNNGC 3 cut(s) 566, 813, 1347
BstDEI CTNAG 7 cut(s) 677, 825, 876, 1248, 1326, 1341, 1503
BstF5I GGATG 4 cut(s) 128, 515, 1027, 1261
BstH2I RGCGCY 1 cut(s) 1155
BstHHI GCGC 2 cut(s) 1154, 1276
BstKTI GATC 3 cut(s) 259, 315, 645
BstMAI GTCTC 4 cut(s) 175, 449, 465, 1164
BstMBI GATC 3 cut(s) 256, 312, 642
BstMCI CGRYCG 1 cut(s) 1437
BstMWI GCNNNNNNNGC 2 cut(s) 269, 361
BstNI CCWGG 7 cut(s) 56, 209, 253, 1019, 1266, 1380, 1467
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 7 cut(s) 54, 207, 251, 1017, 1264, 1378, 1465
BstSFI CTRYAG 3 cut(s) 184, 534, 980
BstSLI GKGCMC 2 cut(s) 796, 1388
BstV1I GCAGC 8 cut(s) 124, 361, 364, 741, 844, 847, 984, 994
BstX2I RGATCY 1 cut(s) 256
BstXI CCANNNNNNTGG 1 cut(s) 789
BstYI RGATCY 1 cut(s) 256
BsuI GTATCC 4 cut(s) 325, 678, 1386, 1423
BsuRI GGCC 3 cut(s) 930, 1179, 1386
BtsCI GGATG 4 cut(s) 128, 515, 1027, 1261
BtsI GCAGTG 1 cut(s) 1032
BtsIMutI CAGTG 4 cut(s) 804, 1032, 1146, 1398
BveI ACCTGC 2 cut(s) 382, 987
Cac8I GCNNGC 3 cut(s) 566, 813, 1347
CaiI CAGNNNCTG 1 cut(s) 1115
CfoI GCGC 2 cut(s) 1154, 1276
Cfr10I RCCGGY 1 cut(s) 919
Cfr13I GGNCC 5 cut(s) 821, 872, 1384, 1385, 1451
CseI GACGC 1 cut(s) 143
CspCI CAANNNNNGTGG 2 cut(s) 304, 339
CviAII CATG 9 cut(s) 299, 403, 502, 559, 689, 710, 868, 1100, 1549
DdeI CTNAG 7 cut(s) 677, 825, 876, 1248, 1326, 1341, 1503
DpnI GATC 3 cut(s) 258, 314, 644
DpnII GATC 3 cut(s) 256, 312, 642
DrdI GACNNNNNNGTC 1 cut(s) 479
DseDI GACNNNNNNGTC 1 cut(s) 479
Eam1104I CTCTTC 3 cut(s) 744, 1082, 1560
EarI CTCTTC 3 cut(s) 744, 1082, 1560
Eco130I CCWWGG 1 cut(s) 782
Eco24I GRGCYC 1 cut(s) 1388
Eco47I GGWCC 3 cut(s) 821, 872, 1451
Eco57I CTGAAG 1 cut(s) 1202
Eco88I CYCGRG 1 cut(s) 1318
EcoO109I RGGNCCY 3 cut(s) 872, 1384, 1385
EcoRII CCWGG 7 cut(s) 54, 207, 251, 1017, 1264, 1378, 1465
EcoT14I CCWWGG 1 cut(s) 782
EcoT38I GRGCYC 1 cut(s) 1388
ErhI CCWWGG 1 cut(s) 782
FaeI CATG 9 cut(s) 302, 406, 505, 562, 692, 713, 871, 1103, 1552
FaqI GGGAC 1 cut(s) 227
FatI CATG 9 cut(s) 298, 402, 501, 558, 688, 709, 867, 1099, 1548
FauI CCCGC 1 cut(s) 268
Fnu4HI GCNGC 9 cut(s) 138, 264, 350, 353, 755, 833, 836, 983, 998
FokI GGATG 4 cut(s) 135, 502, 1034, 1268
FriOI GRGCYC 1 cut(s) 1388
Fsp4HI GCNGC 9 cut(s) 138, 264, 350, 353, 755, 833, 836, 983, 998
FspBI CTAG 2 cut(s) 273, 441
GlaI GCGC 2 cut(s) 1153, 1275
GluI GCNGC 9 cut(s) 138, 264, 350, 353, 755, 833, 836, 983, 998
GsuI CTGGAG 2 cut(s) 1001, 1374
HaeII RGCGCY 1 cut(s) 1155
HaeIII GGCC 3 cut(s) 930, 1179, 1386
HapII CCGG 3 cut(s) 899, 920, 934
HgaI GACGC 1 cut(s) 143
HhaI GCGC 2 cut(s) 1154, 1276
Hin1II CATG 9 cut(s) 302, 406, 505, 562, 692, 713, 871, 1103, 1552
Hin6I GCGC 2 cut(s) 1152, 1274
HinP1I GCGC 2 cut(s) 1152, 1274
HincII GTYRAC 1 cut(s) 889
HindII GTYRAC 1 cut(s) 889
HindIII AAGCTT 2 cut(s) 580, 878
HinfI GANTC 5 cut(s) 168, 488, 1172, 1460, 1527
HpaI GTTAAC 1 cut(s) 889
HpaII CCGG 3 cut(s) 899, 920, 934
HphI GGTGA 5 cut(s) 169, 818, 934, 968, 1455
Hpy166II GTNNAC 3 cut(s) 114, 889, 1357
Hpy188I TCNGA 6 cut(s) 16, 43, 387, 627, 828, 1057
Hpy188III TCNNGA 5 cut(s) 197, 545, 1044, 1284, 1318
Hpy8I GTNNAC 3 cut(s) 114, 889, 1357
HpyAV CCTTC 4 cut(s) 37, 391, 565, 1018
HpyCH4III ACNGT 4 cut(s) 535, 622, 858, 1061
HpyCH4V TGCA 8 cut(s) 428, 564, 757, 848, 982, 1025, 1107, 1573
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 361
HpyF3I CTNAG 7 cut(s) 677, 825, 876, 1248, 1326, 1341, 1503
Hsp92II CATG 9 cut(s) 302, 406, 505, 562, 692, 713, 871, 1103, 1552
HspAI GCGC 2 cut(s) 1152, 1274
KspAI GTTAAC 1 cut(s) 889
Kzo9I GATC 3 cut(s) 256, 312, 642
LguI GCTCTTC 2 cut(s) 744, 1082
LmnI GCTCC 3 cut(s) 43, 1020, 1117
Lsp1109I GCAGC 8 cut(s) 124, 361, 364, 741, 844, 847, 984, 994
LweI GCATC 6 cut(s) 415, 766, 857, 994, 1012, 1560
MaeI CTAG 2 cut(s) 273, 441
MaeIII GTNAC 3 cut(s) 221, 922, 1144
MalI GATC 3 cut(s) 258, 314, 644
MboI GATC 3 cut(s) 256, 312, 642
MboII GAAGA 5 cut(s) 719, 761, 1099, 1195, 1577
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 2 cut(s) 796, 1388
MluCI AATT 7 cut(s) 47, 392, 554, 744, 761, 972, 1290
MlyI GAGTC 4 cut(s) 177, 497, 1166, 1536
MmeI TCCRAC 4 cut(s) 21, 55, 394, 1147
MroXI GAANNNNTTC 2 cut(s) 1077, 1091
MseI TTAA 4 cut(s) 552, 717, 888, 1289
MslI CAYNNNNRTG 1 cut(s) 693
MspI CCGG 3 cut(s) 899, 920, 934
MspR9I CCNGG 7 cut(s) 56, 209, 253, 1019, 1266, 1380, 1467
MunI CAATTG 1 cut(s) 744
MvaI CCWGG 7 cut(s) 56, 209, 253, 1019, 1266, 1380, 1467
MwoI GCNNNNNNNGC 2 cut(s) 269, 361
NdeII GATC 3 cut(s) 256, 312, 642
NlaIII CATG 9 cut(s) 302, 406, 505, 562, 692, 713, 871, 1103, 1552
NlaIV GGNNCC 6 cut(s) 258, 795, 873, 1261, 1385, 1386
NmeAIII GCCGAG 1 cut(s) 1346
NmuCI GTSAC 3 cut(s) 221, 922, 1144
NspI RCATGY 1 cut(s) 302
NspV TTCGAA 1 cut(s) 1071
PaeR7I CTCGAG 1 cut(s) 1318
PaqCI CACCTGC 1 cut(s) 987
PciI ACATGT 1 cut(s) 298
PciSI GCTCTTC 2 cut(s) 744, 1082
PdmI GAANNNNTTC 2 cut(s) 1077, 1091
PfeI GAWTC 1 cut(s) 1460
PflMI CCANNNNNTGG 2 cut(s) 61, 803
PfoI TCCNGGA 1 cut(s) 1017
PkrI GCNGC 9 cut(s) 139, 265, 351, 354, 756, 834, 837, 984, 999
PleI GAGTC 4 cut(s) 176, 496, 1166, 1535
PpsI GAGTC 4 cut(s) 176, 496, 1166, 1535
PpuMI RGGWCCY 1 cut(s) 872
PscI ACATGT 1 cut(s) 298
PshBI ATTAAT 2 cut(s) 717, 1289
PsiI TTATAA 1 cut(s) 735
Psp5II RGGWCCY 1 cut(s) 872
Psp6I CCWGG 7 cut(s) 54, 207, 251, 1017, 1264, 1378, 1465
PspGI CCWGG 7 cut(s) 54, 207, 251, 1017, 1264, 1378, 1465
PspN4I GGNNCC 6 cut(s) 258, 795, 873, 1261, 1385, 1386
PspOMI GGGCCC 1 cut(s) 1384
PspPI GGNCC 5 cut(s) 821, 872, 1384, 1385, 1451
PspPPI RGGWCCY 1 cut(s) 872
PstI CTGCAG 1 cut(s) 984
PstNI CAGNNNCTG 1 cut(s) 1115
PsuI RGATCY 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 693
SapI GCTCTTC 2 cut(s) 744, 1082
SaqAI TTAA 4 cut(s) 552, 717, 888, 1289
SatI GCNGC 9 cut(s) 138, 264, 350, 353, 755, 833, 836, 983, 998
Sau3AI GATC 3 cut(s) 256, 312, 642
Sau96I GGNCC 5 cut(s) 821, 872, 1384, 1385, 1451
SchI GAGTC 4 cut(s) 177, 497, 1166, 1536
ScrFI CCNGG 7 cut(s) 56, 209, 253, 1019, 1266, 1380, 1467
SduI GDGCHC 2 cut(s) 796, 1388
SfaNI GCATC 6 cut(s) 415, 766, 857, 994, 1012, 1560
SfcI CTRYAG 3 cut(s) 184, 534, 980
Sfr274I CTCGAG 1 cut(s) 1318
SfuI TTCGAA 1 cut(s) 1071
SinI GGWCC 3 cut(s) 821, 872, 1451
SlaI CTCGAG 1 cut(s) 1318
SmiMI CAYNNNNRTG 1 cut(s) 693
SmlI CTYRAG 1 cut(s) 1318
SmoI CTYRAG 1 cut(s) 1318
Sse9I AATT 7 cut(s) 47, 392, 554, 744, 761, 972, 1290
SsiI CCGC 8 cut(s) 4, 261, 264, 453, 568, 740, 843, 1454
SspMI CTAG 2 cut(s) 273, 441
StyD4I CCNGG 7 cut(s) 54, 207, 251, 1017, 1264, 1378, 1465
StyI CCWWGG 1 cut(s) 782
TaaI ACNGT 4 cut(s) 535, 622, 858, 1061
TaqI TCGA 4 cut(s) 198, 1045, 1071, 1319
TaqII GACCGA 1 cut(s) 1451
TasI AATT 7 cut(s) 47, 392, 554, 744, 761, 972, 1290
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 1 cut(s) 1460
Tru1I TTAA 4 cut(s) 552, 717, 888, 1289
Tru9I TTAA 4 cut(s) 552, 717, 888, 1289
TscAI CASTG 4 cut(s) 804, 1032, 1153, 1398
TseFI GTSAC 3 cut(s) 221, 922, 1144
TseI GCWGC 8 cut(s) 137, 349, 352, 754, 832, 835, 982, 997
Tsp45I GTSAC 3 cut(s) 221, 922, 1144
TspDTI ATGAA 5 cut(s) 547, 856, 1041, 1196, 1578
TspGWI ACGGA 1 cut(s) 1129
TspRI CASTG 4 cut(s) 804, 1032, 1153, 1398
Van91I CCANNNNNTGG 2 cut(s) 61, 803
VpaK11BI GGWCC 3 cut(s) 821, 872, 1451
VspI ATTAAT 2 cut(s) 717, 1289
XapI RAATTY 1 cut(s) 554
XceI RCATGY 1 cut(s) 302
XhoI CTCGAG 1 cut(s) 1318
XmnI GAANNNNTTC 2 cut(s) 1077, 1091
XspI CTAG 2 cut(s) 273, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.