pycom15g19140

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013016
Physical Location & Seq
Reverse (-)
13644085 .. 13645203
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 435 bp
ATGCGGTTGCAGTGGATAATTCTCGAGGCAGATTCATCAATTCAGACGATTATGGAGAAGTTACAGTCTTACAAATCAAGGGTCTCGCTTTATTTTGAGGGGTTTCATTATCAACTCGGGGACTTCCAATTGAGAGTGGGGAAAGTTATTCCTACTCATTCAGAAAATTTGAGAGGAATAGTTATGGAGCTGGAGTACCTTCCCATTTCTTCGATGGAAAAAGCGAGGCAAGTCATGGAAGAGTTCATGGATATTTGGCAGCAGGCTTTGGCGAAAAGATCATTGCCAGGACACTTCATGCATGTGGAACCAAACTTTTCCGAGTATGGCCTTGGAGACCAATATACTTCACAGCATACTGCTGTCCAGTATGCTGCCATTATGGCTCAACTCATTGCAACCGTTCAAGCAGTGCAGCAATCGGCAAGAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002831 GO:0003674 GO:0003712 GO:0003713 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0010646 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016592 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0031047 GO:0031050 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043900 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0097659 GO:0140110 GO:1900150 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902680 GO:1903506 GO:1903508 GO:2000031 GO:2000112 GO:2001023 GO:2001038 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med20

Protein Analysis

145

Amino Acids

16.64

Weight (kDa)

5.92

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med20 PF08612 7 - 128 1.2e-31 TATA-binding related factor (TRF) of subunit 20 of Mediator complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28230 AT2G28230 AT4G09070
fragaria_vesca FvH4_1g08420
malus_domestica MD15G1215700.v1.1 MD15G1215800.v1.1
prunus_persica Prupe.7G201200_v2.0.a1
pyrus_communis pycom02g07100 pycom15g19140
rosa_chinensis RchiOBHm_Chr2g0094521
rosa_laevigata RLG00000016498
rosa_multiflora Rmu_sc0004359.1_g000043
rosa_roxburghii Rroxscaffold_2G00147100
rosa_rugosa Rorug02G0044400
rosa_samantha Rh2AG092700 Rh2BG092900 Rh2CG095400 Rh2DG091800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 4
AcsI RAATTY 1 cut(s) 166
AfaI GTAC 1 cut(s) 197
AgsI TTSAA 1 cut(s) 407
AjnI CCWGG 1 cut(s) 286
AluBI AGCT 1 cut(s) 190
AluI AGCT 1 cut(s) 190
Alw26I GTCTC 2 cut(s) 88, 330
Ama87I CYCGRG 2 cut(s) 23, 116
AoxI GGCC 1 cut(s) 328
ApeKI GCWGC 3 cut(s) 259, 374, 415
ApoI RAATTY 1 cut(s) 166
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
AvaI CYCGRG 2 cut(s) 23, 116
BbvI GCAGC 3 cut(s) 271, 361, 427
BccI CCATC 1 cut(s) 208
BciT130I CCWGG 1 cut(s) 288
BcoDI GTCTC 2 cut(s) 88, 330
BglI GCCNNNNNGGC 1 cut(s) 383
BisI GCNGC 3 cut(s) 260, 375, 416
BlsI GCNGC 3 cut(s) 261, 376, 417
Bme1390I CCNGG 1 cut(s) 288
BmeT110I CYCGRG 2 cut(s) 23, 116
BmiI GGNNCC 1 cut(s) 309
BmrFI CCNGG 1 cut(s) 288
BpmI CTGGAG 1 cut(s) 212
BsaI GGTCTC 2 cut(s) 88, 330
BsaJI CCNNGG 1 cut(s) 331
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bse1I ACTGG 1 cut(s) 367
Bse3DI GCAATG 2 cut(s) 281, 393
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 1 cut(s) 331
BseMI GCAATG 2 cut(s) 281, 393
BseNI ACTGG 1 cut(s) 367
BseXI GCAGC 3 cut(s) 271, 361, 427
BsgI GTGCAG 1 cut(s) 434
BshFI GGCC 1 cut(s) 330
BsiHKCI CYCGRG 2 cut(s) 23, 116
BslFI GGGAC 1 cut(s) 134
BsmAI GTCTC 2 cut(s) 88, 330
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 1 cut(s) 330
Bso31I GGTCTC 2 cut(s) 88, 330
BsoBI CYCGRG 2 cut(s) 23, 116
Bsp143I GATC 1 cut(s) 278
BspACI CCGC 1 cut(s) 4
BspANI GGCC 1 cut(s) 330
BspLI GGNNCC 1 cut(s) 309
BspTNI GGTCTC 2 cut(s) 88, 330
BsrDI GCAATG 2 cut(s) 281, 393
BsrI ACTGG 1 cut(s) 367
BssECI CCNNGG 1 cut(s) 331
BssMI GATC 1 cut(s) 278
BssT1I CCWWGG 1 cut(s) 331
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 2 cut(s) 66, 403
Bst6I CTCTTC 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 264
BstKTI GATC 1 cut(s) 281
BstMAI GTCTC 2 cut(s) 88, 330
BstMBI GATC 1 cut(s) 278
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstNI CCWGG 1 cut(s) 288
BstNSI RCATGY 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 286
BstV1I GCAGC 3 cut(s) 271, 361, 427
BsuRI GGCC 1 cut(s) 330
BtsI GCAGTG 2 cut(s) 17, 417
BtsIMutI CAGTG 2 cut(s) 17, 417
Cac8I GCNNGC 1 cut(s) 264
Csp6I GTAC 1 cut(s) 196
CviAII CATG 4 cut(s) 235, 247, 298, 302
CviJI RGCY 4 cut(s) 190, 266, 330, 386
CviKI_1 RGCY 4 cut(s) 190, 266, 330, 386
CviQI GTAC 1 cut(s) 196
DpnI GATC 1 cut(s) 280
DpnII GATC 1 cut(s) 278
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 331
Eco31I GGTCTC 2 cut(s) 88, 330
Eco88I CYCGRG 2 cut(s) 23, 116
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 331
EcoT22I ATGCAT 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 331
FaeI CATG 4 cut(s) 238, 250, 301, 305
FaqI GGGAC 1 cut(s) 134
FatI CATG 4 cut(s) 234, 246, 297, 301
Fnu4HI GCNGC 3 cut(s) 260, 375, 416
Fsp4HI GCNGC 3 cut(s) 260, 375, 416
GluI GCNGC 3 cut(s) 260, 375, 416
GsuI CTGGAG 1 cut(s) 212
HaeIII GGCC 1 cut(s) 330
Hin1II CATG 4 cut(s) 238, 250, 301, 305
HinfI GANTC 1 cut(s) 32
Hpy188I TCNGA 3 cut(s) 45, 163, 322
Hpy188III TCNNGA 1 cut(s) 23
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 2 cut(s) 66, 403
HpyCH4V TGCA 4 cut(s) 10, 301, 398, 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
Hsp92II CATG 4 cut(s) 238, 250, 301, 305
Kzo9I GATC 1 cut(s) 278
LmnI GCTCC 1 cut(s) 187
LpnPI CCDG 5 cut(s) 176, 248, 273, 300, 380
Lsp1109I GCAGC 3 cut(s) 271, 361, 427
MaeIII GTNAC 1 cut(s) 60
MalI GATC 1 cut(s) 280
MboI GATC 1 cut(s) 278
MboII GAAGA 2 cut(s) 201, 251
MfeI CAATTG 1 cut(s) 128
MluCI AATT 5 cut(s) 18, 39, 128, 166, 430
MnlI CCTC 4 cut(s) 19, 91, 167, 219
Mph1103I ATGCAT 1 cut(s) 303
MslI CAYNNNNRTG 1 cut(s) 302
MspR9I CCNGG 1 cut(s) 288
MunI CAATTG 1 cut(s) 128
MvaI CCWGG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 383
NdeII GATC 1 cut(s) 278
NlaIII CATG 4 cut(s) 238, 250, 301, 305
NlaIV GGNNCC 1 cut(s) 309
NsiI ATGCAT 1 cut(s) 303
NspI RCATGY 1 cut(s) 305
PaeR7I CTCGAG 1 cut(s) 23
PfeI GAWTC 1 cut(s) 32
PkrI GCNGC 3 cut(s) 261, 376, 417
Psp6I CCWGG 1 cut(s) 286
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 1 cut(s) 309
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 1 cut(s) 302
SatI GCNGC 3 cut(s) 260, 375, 416
Sau3AI GATC 1 cut(s) 278
ScrFI CCNGG 1 cut(s) 288
SetI ASST 2 cut(s) 192, 201
Sfr274I CTCGAG 1 cut(s) 23
SlaI CTCGAG 1 cut(s) 23
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 5 cut(s) 18, 39, 128, 166, 430
SsiI CCGC 1 cut(s) 4
StyD4I CCNGG 1 cut(s) 286
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 2 cut(s) 66, 403
TaqI TCGA 2 cut(s) 24, 212
TasI AATT 5 cut(s) 18, 39, 128, 166, 430
TfiI GAWTC 1 cut(s) 32
TscAI CASTG 2 cut(s) 17, 417
TseI GCWGC 3 cut(s) 259, 374, 415
TspDTI ATGAA 4 cut(s) 24, 95, 235, 286
TspRI CASTG 2 cut(s) 17, 417
XapI RAATTY 1 cut(s) 166
XceI RCATGY 1 cut(s) 305
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XhoI CTCGAG 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.