pycom15g21400

Protein of unknown function (DUF630)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011005
Physical Location & Seq
Reverse (-)
15374124 .. 15377574
3451 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2217 bp
ATGGGGTGCTGCTATTCGAGGGTAGAACGGGAAGAAATGGTGTCGCGATGCAGATCGAGAAAGCGGTACATGAAGCAGCTGGTGAAAGCACGGCAAGCTTGGTCGGCGGCTCACACAATGTACCTCCGCTCTCTCCGCAGCACCGGCTCGGCTCTGCTCCAGTTTTCAAATGCCGAAACTACCCTCCACCACCACCTCAACCACAACCACCACAACAATAGTTACCGCTCAGCGCCGCCGCCGCCGCTACAAACCCCGCCTACCCCACAACCACCGCCACCGCCACCTCCACTCAGCTCAAGCTCCGACTCTTGGACAACATCCACCACGCCCTCAACAGCTCTTCCACCTCCTCCTCCGCCGCCGCCGTCGTCCACCTGGGATTTCTGGGACCCATTTGTGGCTTCCTCTTCGAGGGCAGTAACGGAAGAAGGGTGGGAGGCTACGACCAGCGCCTCGGAGGCGGTGGTCACCGTCACGGCGGTGAGCACGGCTCCTCCACCGTCTGTGGTGAGTGAGTTTTCCAAAGAGAGCGGCACGACGAGCGAGCTGGCGATGGTAGTGTCGAGAAATGCTAAGGATCTGATTGAGATTATAAAGGAGCTGGATGAGTATTTCCTCAAGGCGGCTGATGCCGGCGGGCTTCTCTCATCGCTGTTGGAAGTTCCCATATTCTCTTCTTCTCAGACCAAAGGAGGTAAAAGATTTGATTTTTACTGTTGTTTGGAGGCCATCCTGCAACAGTCGAAGTGCAATTTCTCCATTTTGTTTTTATCAACGACAACTCAAGGACGACATTTTGGAGTGCCAATAACATTCCTTTTACTCTTTGTGCGTTTTAATGCAATAGACGGATTTTCTTTGCAGGTAAAGTTTATCGATCATGGGTGTAATTTGAGTCCGTCGCTGTGGACATGGGGAAGTTCAAGTCAAAAGTCTGGAGTATTCGGAAAAATGGGTTGTGACGAGATGGTGCTTAGTCAAGTAGGCAGTGGGGTTGGAGATGAAGGTGTGGTGAGTAGCAGCCATTGTTCTACTGTGGAGAGGCTTTATGCATGGGAAAAGAAACTATACCAGGAGGTCAAGAGTAACGCTAGAGTCGCCAACTTATTAAACCAGCTTTTGGATACCAATGTATGTGGCAGTGATGCAGAGACACTAAAGGTAGAGCATGAGAAGCGGGTGTCAACACTGAAGAAGCTAGAGATGAAGAGGGGAGACTATGTGAAGACAGAGAAGACGAAGAAAGAGGTGGAGAAGTTGGAGTCACAAATGATGGTTTCTTCACAGGCCATTGATACAACTTCAGCTGAAATCATCAACCTTCGGGAGACTGAGCTCTACCCTCAACTCATTGAGCTTGTCAAAGGTGTATTATCATACACCAGTTCCAAAAGGAATCAATTTACAGTTGACGGAATATATTTGCTTTACAGATTGATGTGCATGTGGAGAAGCATGTACGAATGCCACCAGGTCCAAAAGCATATTGTTCAGCAGCTCAAATACCTCAACACCATACCATCTACCGAACCCACGTCTGAGATTCACCGGCAAGCAACACTGCAGCTCGAGCTTCAAGTCCAGCAATGGCACTTATCATTTTGCAGCCTGGTCAAGGCTCAGCGTGACTATATCCAGTCTCTCACAGGATGGCTCCGTCTCAGCCTTTTCCAGTTTCGTAGACATCCAATAGCAAAGACAAGTCAAGAATCAAGAATTTACACGCTTTGCGAAGAATGGCACCATGCAATTGACCGGATTCCGGACAAAGTAGCATCTGAAGGAATCAAGAGCTTTTTAACCGTAGTTCATGCTATTGTAGTTCAACAAGCAGACGAATACAAGCAAAAACGGAAGTCAGAATCTACTTTTAAACAGCTTGAGAAGAAGGCAACTGCGCTTAGATCGCTGGAGTCCAAGTATGGCCCCTACTCAATGCCTGATTCCTCCAGCAATACTAAGAAGAGCCCAGTTGCTGAGAAGCGAGCAAAGGTGGAGGTCATTAAAGCAAAAGCGGAAGAGGAGAAGAGCAAGCATGAAAAGTTAGTGAGTGTGACAAGGACCATGACAATAAATAACTTGCAGATGGGGTTTCCACATGTCTTTGAGGCGATGGTCGGGTTTTCAAGTGTGTGTATGCAGGCTTTCGAGCAACTATATAACCAAGCGAAAAGTGCTAATGAGGAGCTTGATGTTAAGAGGTTACTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

739

Amino Acids

83.07

Weight (kDa)

8.73

Isoelectric Point (pI)

54.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 1 - 59 5.1e-21 Protein of unknown function (DUF630)
DUF632 PF04782 195 - 367 1.6e-19 Protein of unknown function (DUF632)
DUF632 PF04782 384 - 459 5.3e-22 Protein of unknown function (DUF632)
DUF632 PF04782 476 - 605 4.4e-46 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011005)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51290 AT3G51290 AT3G51290
fragaria_vesca FvH4_1g11480 FvH4_1g11480
malus_domestica MD15G1242800.v1.1
prunus_persica Prupe.7G171300_v2.0.a1 Prupe.7G171300_v2.0.a1
pyrus_communis pycom02g09980 pycom15g21400
rosa_chinensis RchiOBHm_Chr2g0099091
rosa_laevigata RLG00000016847
rosa_multiflora Rmu_sc0012098.1_g000004
rosa_roxburghii Rroxscaffold_2G00143450
rosa_rugosa Rorug02G0076600
rosa_samantha Rh2AG125700 Rh2BG129000 Rh2CG130000 Rh2DG130400
rosa_wichuraiana Rw2G009680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 596
Acc36I ACCTGC 1 cut(s) 856
AccB1I GGYRCC 1 cut(s) 1743
AccB7I CCANNNNNTGG 1 cut(s) 1123
AccBSI CCGCTC 3 cut(s) 129, 228, 534
AccI GTMKAC 1 cut(s) 1684
AccII CGCG 1 cut(s) 46
AccIII TCCGGA 1 cut(s) 1765
AclWI GGATC 1 cut(s) 588
AcsI RAATTY 1 cut(s) 1719
AcuI CTGAAG 3 cut(s) 1214, 1290, 1803
AfaI GTAC 3 cut(s) 68, 122, 1463
AfiI CCNNNNNNNGG 7 cut(s) 312, 400, 414, 625, 1123, 1618, 1765
AflIII ACRYGT 1 cut(s) 2101
AgsI TTSAA 5 cut(s) 168, 927, 1580, 1829, 2130
AjiI CACGTC 1 cut(s) 1539
AjnI CCWGG 4 cut(s) 377, 1074, 1473, 1611
AleI CACNNNNGTG 1 cut(s) 482
Alw21I GWGCWC 2 cut(s) 491, 1341
Alw26I GTCTC 5 cut(s) 1148, 1212, 1325, 1647, 1667
AlwI GGATC 1 cut(s) 588
AlwNI CAGNNNCTG 1 cut(s) 1979
Ama87I CYCGRG 1 cut(s) 1571
Aor13HI TCCGGA 1 cut(s) 1765
AoxI GGCC 3 cut(s) 729, 1290, 1927
ApeKI GCWGC 7 cut(s) 9, 76, 138, 1023, 1498, 1567, 1608
ApoI RAATTY 1 cut(s) 1719
AspLEI GCGC 3 cut(s) 235, 455, 1903
AspS9I GGNCC 4 cut(s) 391, 1477, 1928, 2064
AsuHPI GGTGA 6 cut(s) 94, 463, 496, 523, 1027, 1541
AvaI CYCGRG 1 cut(s) 1571
AvaII GGWCC 3 cut(s) 391, 1477, 2064
BaeI ACNNNNGTAYC 2 cut(s) 1119, 1152
BanI GGYRCC 1 cut(s) 1743
BanII GRGCYC 2 cut(s) 1341, 1973
BbsI GAAGAC 2 cut(s) 1235, 1244
Bbv12I GWGCWC 2 cut(s) 491, 1341
BbvI GCAGC 6 cut(s) 88, 150, 1035, 1510, 1579, 1620
BccI CCATC 8 cut(s) 550, 740, 964, 1270, 1531, 1647, 2083, 2110
BceAI ACGGC 4 cut(s) 107, 352, 495, 507
BciT130I CCWGG 4 cut(s) 379, 1076, 1475, 1613
BciVI GTATCC 1 cut(s) 1120
BcoDI GTCTC 5 cut(s) 1148, 1212, 1325, 1647, 1667
BfaI CTAG 2 cut(s) 1095, 1202
BfmI CTRYAG 1 cut(s) 1565
BfoI RGCGCY 2 cut(s) 236, 456
BfuAI ACCTGC 1 cut(s) 856
BfuI GTATCC 1 cut(s) 1120
BglI GCCNNNNNGGC 1 cut(s) 461
BlpI GCTNAGC 2 cut(s) 229, 1623
Bme1390I CCNGG 4 cut(s) 379, 1076, 1475, 1613
Bme18I GGWCC 3 cut(s) 391, 1477, 2064
BmeT110I CYCGRG 1 cut(s) 1571
BmgBI CACGTC 1 cut(s) 1539
BmgT120I GGNCC 4 cut(s) 391, 1477, 1928, 2064
BmiI GGNNCC 6 cut(s) 392, 393, 495, 1658, 1745, 1930
BmrFI CCNGG 4 cut(s) 379, 1076, 1475, 1613
BmrI ACTGGG 1 cut(s) 1967
BmsI GCATC 4 cut(s) 38, 622, 1138, 1787
BmuI ACTGGG 1 cut(s) 1967
BpiI GAAGAC 2 cut(s) 1235, 1244
BplI GAGNNNNNCTC 2 cut(s) 478, 510
BpmI CTGGAG 4 cut(s) 143, 960, 1934, 1936
Bpu10I CCTNAGC 1 cut(s) 576
Bpu1102I GCTNAGC 2 cut(s) 229, 1623
BpuEI CTTGAG 4 cut(s) 283, 605, 771, 1904
Bsa29I ATCGAT 1 cut(s) 879
BsaBI GATNNNNATC 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 378, 456
BsaWI WCCGGW 2 cut(s) 1758, 1765
BsaXI ACNNNNNCTCC 6 cut(s) 481, 511, 1209, 1239, 1907, 1937
Bsc4I CCNNNNNNNGG 7 cut(s) 312, 400, 414, 625, 1123, 1618, 1765
Bse118I RCCGGY 3 cut(s) 143, 635, 1551
Bse1I ACTGG 5 cut(s) 160, 1386, 1639, 1675, 1973
Bse3DI GCAATG 1 cut(s) 1595
Bse8I GATNNNNATC 1 cut(s) 52
BseAI TCCGGA 1 cut(s) 1765
BseBI CCWGG 4 cut(s) 379, 1076, 1475, 1613
BseCI ATCGAT 1 cut(s) 879
BseDI CCNNGG 2 cut(s) 378, 456
BseGI GGATG 5 cut(s) 320, 613, 732, 1658, 1687
BseJI GATNNNNATC 1 cut(s) 52
BseLI CCNNNNNNNGG 7 cut(s) 312, 400, 414, 625, 1123, 1618, 1765
BseMI GCAATG 1 cut(s) 1595
BseMII CTCAG 8 cut(s) 243, 307, 698, 1326, 1533, 1637, 1678, 1971
BseNI ACTGG 5 cut(s) 160, 1386, 1639, 1675, 1973
BseRI GAGGAG 5 cut(s) 342, 345, 486, 2039, 2201
BseXI GCAGC 6 cut(s) 88, 150, 1035, 1510, 1579, 1620
Bsh1236I CGCG 1 cut(s) 46
BshFI GGCC 3 cut(s) 731, 1292, 1929
BshNI GGYRCC 1 cut(s) 1743
BshVI ATCGAT 1 cut(s) 879
BsiHKAI GWGCWC 2 cut(s) 491, 1341
BsiHKCI CYCGRG 1 cut(s) 1571
BsiSI CCGG 5 cut(s) 144, 636, 1552, 1759, 1766
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 7 cut(s) 312, 400, 414, 625, 1123, 1618, 1765
BsmAI GTCTC 5 cut(s) 1148, 1212, 1325, 1647, 1667
BsmBI CGTCTC 1 cut(s) 1667
BsmFI GGGAC 1 cut(s) 404
BsmI GAATGC 1 cut(s) 1472
BsnI GGCC 3 cut(s) 731, 1292, 1929
BsoBI CYCGRG 1 cut(s) 1571
Bsp1286I GDGCHC 3 cut(s) 491, 1341, 1973
Bsp13I TCCGGA 1 cut(s) 1765
Bsp143I GATC 4 cut(s) 53, 580, 880, 1907
Bsp1720I GCTNAGC 2 cut(s) 229, 1623
Bsp68I TCGCGA 1 cut(s) 46
BspANI GGCC 3 cut(s) 731, 1292, 1929
BspCNI CTCAG 8 cut(s) 242, 306, 697, 1327, 1534, 1636, 1677, 1972
BspDI ATCGAT 1 cut(s) 879
BspEI TCCGGA 1 cut(s) 1765
BspFNI CGCG 1 cut(s) 46
BspLI GGNNCC 6 cut(s) 392, 393, 495, 1658, 1745, 1930
BspMAI CTGCAG 1 cut(s) 1569
BspMI ACCTGC 1 cut(s) 856
BspPI GGATC 1 cut(s) 588
BspQI GCTCTTC 3 cut(s) 348, 1961, 2024
BspT107I GGYRCC 1 cut(s) 1743
BsrBI CCGCTC 3 cut(s) 129, 228, 534
BsrDI GCAATG 1 cut(s) 1595
BsrFI RCCGGY 3 cut(s) 143, 635, 1551
BsrI ACTGG 5 cut(s) 160, 1386, 1639, 1675, 1973
BssAI RCCGGY 3 cut(s) 143, 635, 1551
BssECI CCNNGG 2 cut(s) 378, 456
BssMI GATC 4 cut(s) 53, 580, 880, 1907
Bst2UI CCWGG 4 cut(s) 379, 1076, 1475, 1613
Bst4CI ACNGT 7 cut(s) 475, 504, 719, 744, 1039, 1411, 1807
Bst6I CTCTTC 7 cut(s) 348, 415, 682, 1205, 1961, 2016, 2024
BstC8I GCNNGC 9 cut(s) 96, 548, 552, 637, 641, 1557, 1989, 2036, 2145
BstEII GGTNACC 1 cut(s) 469
BstENI CCTNNNNNAGG 2 cut(s) 412, 1616
BstF5I GGATG 5 cut(s) 320, 613, 732, 1658, 1687
BstFNI CGCG 1 cut(s) 46
BstH2I RGCGCY 2 cut(s) 236, 456
BstHHI GCGC 3 cut(s) 235, 455, 1903
BstKTI GATC 4 cut(s) 56, 583, 883, 1910
BstMAI GTCTC 5 cut(s) 1148, 1212, 1325, 1647, 1667
BstMBI GATC 4 cut(s) 53, 580, 880, 1907
BstNI CCWGG 4 cut(s) 379, 1076, 1475, 1613
BstNSI RCATGY 3 cut(s) 1450, 1462, 2105
BstPI GGTNACC 1 cut(s) 469
BstSCI CCNGG 4 cut(s) 377, 1074, 1473, 1611
BstSFI CTRYAG 1 cut(s) 1565
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 6 cut(s) 88, 150, 1035, 1510, 1579, 1620
BstV2I GAAGAC 2 cut(s) 1235, 1244
BstX2I RGATCY 1 cut(s) 580
BstYI RGATCY 1 cut(s) 580
Bsu15I ATCGAT 1 cut(s) 879
BsuI GTATCC 1 cut(s) 1120
BsuRI GGCC 3 cut(s) 731, 1292, 1929
BsuTUI ATCGAT 1 cut(s) 879
BtgZI GCGATG 4 cut(s) 61, 569, 636, 2129
BtrI CACGTC 1 cut(s) 1539
BtsCI GGATG 5 cut(s) 320, 613, 732, 1658, 1687
BtsI GCAGTG 3 cut(s) 997, 1150, 1562
BtsIMutI CAGTG 4 cut(s) 997, 1150, 1190, 1562
BtuMI TCGCGA 1 cut(s) 46
BveI ACCTGC 1 cut(s) 856
Cac8I GCNNGC 9 cut(s) 96, 548, 552, 637, 641, 1557, 1989, 2036, 2145
CaiI CAGNNNCTG 1 cut(s) 1979
CfoI GCGC 3 cut(s) 235, 455, 1903
Cfr10I RCCGGY 3 cut(s) 143, 635, 1551
Cfr13I GGNCC 4 cut(s) 391, 1477, 1928, 2064
ClaI ATCGAT 1 cut(s) 879
CsiI ACCWGGT 1 cut(s) 1473
Csp6I GTAC 3 cut(s) 67, 121, 1462
CspCI CAANNNNNGTGG 2 cut(s) 1120, 1155
CviQI GTAC 3 cut(s) 67, 121, 1462
DpnI GATC 4 cut(s) 55, 582, 882, 1909
DpnII GATC 4 cut(s) 53, 580, 880, 1907
DraI TTTAAA 1 cut(s) 1876
Eam1104I CTCTTC 7 cut(s) 348, 415, 682, 1205, 1961, 2016, 2024
EarI CTCTTC 7 cut(s) 348, 415, 682, 1205, 1961, 2016, 2024
EciI GGCGGA 1 cut(s) 348
Ecl136II GAGCTC 1 cut(s) 1339
Eco24I GRGCYC 2 cut(s) 1341, 1973
Eco47I GGWCC 3 cut(s) 391, 1477, 2064
Eco53kI GAGCTC 1 cut(s) 1339
Eco57I CTGAAG 3 cut(s) 1214, 1290, 1803
Eco88I CYCGRG 1 cut(s) 1571
Eco91I GGTNACC 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 1339
EcoNI CCTNNNNNAGG 2 cut(s) 412, 1616
EcoO109I RGGNCCY 1 cut(s) 391
EcoO65I GGTNACC 1 cut(s) 469
EcoRII CCWGG 4 cut(s) 377, 1074, 1473, 1611
EcoT22I ATGCAT 1 cut(s) 1057
EcoT38I GRGCYC 2 cut(s) 1341, 1973
Esp3I CGTCTC 1 cut(s) 1667
FaqI GGGAC 1 cut(s) 404
FauI CCCGC 3 cut(s) 264, 632, 1173
FblI GTMKAC 1 cut(s) 1684
FokI GGATG 5 cut(s) 307, 620, 719, 1665, 1674
FriOI GRGCYC 2 cut(s) 1341, 1973
FspBI CTAG 2 cut(s) 1095, 1202
GlaI GCGC 3 cut(s) 234, 454, 1902
GsuI CTGGAG 4 cut(s) 143, 960, 1934, 1936
HaeII RGCGCY 2 cut(s) 236, 456
HaeIII GGCC 3 cut(s) 731, 1292, 1929
HapII CCGG 5 cut(s) 144, 636, 1552, 1759, 1766
HhaI GCGC 3 cut(s) 235, 455, 1903
Hin6I GCGC 3 cut(s) 233, 453, 1901
HinP1I GCGC 3 cut(s) 233, 453, 1901
HincII GTYRAC 2 cut(s) 1188, 1414
HindII GTYRAC 2 cut(s) 1188, 1414
HindIII AAGCTT 1 cut(s) 96
HpaII CCGG 5 cut(s) 144, 636, 1552, 1759, 1766
HphI GGTGA 6 cut(s) 94, 463, 496, 523, 1027, 1541
Hpy166II GTNNAC 5 cut(s) 375, 912, 1188, 1414, 1685
Hpy188I TCNGA 8 cut(s) 307, 460, 585, 687, 950, 1543, 1783, 1864
Hpy8I GTNNAC 5 cut(s) 375, 912, 1188, 1414, 1685
Hpy99I CGWCG 3 cut(s) 373, 544, 907
HpyAV CCTTC 5 cut(s) 425, 1001, 1334, 1778, 1885
HpyCH4III ACNGT 7 cut(s) 475, 504, 719, 744, 1039, 1411, 1807
HpyCH4IV ACGT 1 cut(s) 1538
HpySE526I ACGT 1 cut(s) 1538
HspAI GCGC 3 cut(s) 233, 453, 1901
KflI GGGWCCC 1 cut(s) 391
Kpn2I TCCGGA 1 cut(s) 1765
KroI GCCGGC 1 cut(s) 635
KroNI GCCGGC 1 cut(s) 637
Kzo9I GATC 4 cut(s) 53, 580, 880, 1907
LguI GCTCTTC 3 cut(s) 348, 1961, 2024
LmnI GCTCC 6 cut(s) 162, 308, 499, 601, 1662, 2188
Lsp1109I GCAGC 6 cut(s) 88, 150, 1035, 1510, 1579, 1620
LweI GCATC 4 cut(s) 38, 622, 1138, 1787
MabI ACCWGGT 1 cut(s) 1473
MaeI CTAG 2 cut(s) 1095, 1202
MaeII ACGT 1 cut(s) 1538
MalI GATC 4 cut(s) 55, 582, 882, 1909
MbiI CCGCTC 3 cut(s) 129, 228, 534
MboI GATC 4 cut(s) 53, 580, 880, 1907
MfeI CAATTG 1 cut(s) 1752
MflI RGATCY 1 cut(s) 580
MhlI GDGCHC 3 cut(s) 491, 1341, 1973
MluCI AATT 5 cut(s) 754, 892, 1403, 1719, 1752
MlyI GAGTC 5 cut(s) 302, 907, 1107, 1274, 1925
MmeI TCCRAC 4 cut(s) 330, 639, 979, 1242
Mph1103I ATGCAT 1 cut(s) 1057
MroI TCCGGA 1 cut(s) 1765
MroNI GCCGGC 1 cut(s) 635
MseI TTAA 6 cut(s) 840, 1112, 1802, 1875, 2007, 2199
MslI CAYNNNNRTG 1 cut(s) 482
MspA1I CMGCKG 2 cut(s) 79, 1310
MspI CCGG 5 cut(s) 144, 636, 1552, 1759, 1766
MspR9I CCNGG 4 cut(s) 379, 1076, 1475, 1613
MunI CAATTG 1 cut(s) 1752
Mva1269I GAATGC 1 cut(s) 1472
MvaI CCWGG 4 cut(s) 379, 1076, 1475, 1613
MvnI CGCG 1 cut(s) 46
NaeI GCCGGC 1 cut(s) 637
NdeII GATC 4 cut(s) 53, 580, 880, 1907
NgoMIV GCCGGC 1 cut(s) 635
NlaIV GGNNCC 6 cut(s) 392, 393, 495, 1658, 1745, 1930
NmeAIII GCCGAG 1 cut(s) 128
NmuCI GTSAC 6 cut(s) 469, 475, 962, 1266, 1628, 2056
NruI TCGCGA 1 cut(s) 46
NsiI ATGCAT 1 cut(s) 1057
NspI RCATGY 3 cut(s) 1450, 1462, 2105
OliI CACNNNNGTG 1 cut(s) 482
PaeR7I CTCGAG 1 cut(s) 1571
PciI ACATGT 1 cut(s) 2101
PciSI GCTCTTC 3 cut(s) 348, 1961, 2024
PctI GAATGC 1 cut(s) 1472
PdiI GCCGGC 1 cut(s) 637
PfeI GAWTC 7 cut(s) 1399, 1546, 1712, 1762, 1788, 1865, 1946
PflMI CCANNNNNTGG 1 cut(s) 1123
PleI GAGTC 5 cut(s) 302, 906, 1106, 1273, 1924
PpsI GAGTC 5 cut(s) 302, 906, 1106, 1273, 1924
PpuMI RGGWCCY 1 cut(s) 391
PscI ACATGT 1 cut(s) 2101
PsiI TTATAA 1 cut(s) 596
Psp124BI GAGCTC 1 cut(s) 1341
Psp5II RGGWCCY 1 cut(s) 391
Psp6I CCWGG 4 cut(s) 377, 1074, 1473, 1611
PspEI GGTNACC 1 cut(s) 469
PspGI CCWGG 4 cut(s) 377, 1074, 1473, 1611
PspN4I GGNNCC 6 cut(s) 392, 393, 495, 1658, 1745, 1930
PspPI GGNCC 4 cut(s) 391, 1477, 1928, 2064
PspPPI RGGWCCY 1 cut(s) 391
PspXI VCTCGAGB 1 cut(s) 1571
PstI CTGCAG 1 cut(s) 1569
PstNI CAGNNNCTG 1 cut(s) 1979
PsuI RGATCY 1 cut(s) 580
PvuII CAGCTG 2 cut(s) 79, 1310
RruI TCGCGA 1 cut(s) 46
RsaI GTAC 3 cut(s) 68, 122, 1463
RsaNI GTAC 3 cut(s) 67, 121, 1462
RseI CAYNNNNRTG 1 cut(s) 482
SacI GAGCTC 1 cut(s) 1341
SapI GCTCTTC 3 cut(s) 348, 1961, 2024
SaqAI TTAA 6 cut(s) 840, 1112, 1802, 1875, 2007, 2199
Sau3AI GATC 4 cut(s) 53, 580, 880, 1907
Sau96I GGNCC 4 cut(s) 391, 1477, 1928, 2064
SchI GAGTC 5 cut(s) 302, 907, 1107, 1274, 1925
ScrFI CCNGG 4 cut(s) 379, 1076, 1475, 1613
SduI GDGCHC 3 cut(s) 491, 1341, 1973
SexAI ACCWGGT 1 cut(s) 1473
SfaNI GCATC 4 cut(s) 38, 622, 1138, 1787
SfcI CTRYAG 1 cut(s) 1565
Sfr274I CTCGAG 1 cut(s) 1571
SinI GGWCC 3 cut(s) 391, 1477, 2064
SlaI CTCGAG 1 cut(s) 1571
SmiMI CAYNNNNRTG 1 cut(s) 482
SmlI CTYRAG 5 cut(s) 298, 620, 786, 1571, 1883
SmoI CTYRAG 5 cut(s) 298, 620, 786, 1571, 1883
Sse9I AATT 5 cut(s) 754, 892, 1403, 1719, 1752
SspMI CTAG 2 cut(s) 1095, 1202
SstI GAGCTC 1 cut(s) 1341
StyD4I CCNGG 4 cut(s) 377, 1074, 1473, 1611
TaaI ACNGT 7 cut(s) 475, 504, 719, 744, 1039, 1411, 1807
TaiI ACGT 1 cut(s) 1541
TaqI TCGA 8 cut(s) 17, 56, 413, 566, 746, 879, 1572, 2151
TasI AATT 5 cut(s) 754, 892, 1403, 1719, 1752
TauI GCSGC 9 cut(s) 110, 238, 241, 244, 247, 364, 367, 537, 629
TfiI GAWTC 7 cut(s) 1399, 1546, 1712, 1762, 1788, 1865, 1946
Tru1I TTAA 6 cut(s) 840, 1112, 1802, 1875, 2007, 2199
Tru9I TTAA 6 cut(s) 840, 1112, 1802, 1875, 2007, 2199
TscAI CASTG 4 cut(s) 997, 1150, 1197, 1569
TseFI GTSAC 6 cut(s) 469, 475, 962, 1266, 1628, 2056
TseI GCWGC 7 cut(s) 9, 76, 138, 1023, 1498, 1567, 1608
Tsp45I GTSAC 6 cut(s) 469, 475, 962, 1266, 1628, 2056
TspDTI ATGAA 5 cut(s) 86, 1020, 1223, 1802, 2055
TspGWI ACGGA 6 cut(s) 440, 867, 891, 1431, 1649, 1870
TspRI CASTG 4 cut(s) 997, 1150, 1197, 1569
Van91I CCANNNNNTGG 1 cut(s) 1123
VpaK11BI GGWCC 3 cut(s) 391, 1477, 2064
XagI CCTNNNNNAGG 2 cut(s) 412, 1616
XapI RAATTY 1 cut(s) 1719
XceI RCATGY 3 cut(s) 1450, 1462, 2105
XhoI CTCGAG 1 cut(s) 1571
XmiI GTMKAC 1 cut(s) 1684
XspI CTAG 2 cut(s) 1095, 1202
Zsp2I ATGCAT 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.