Rho (Ras homology) subfamily of Ras-like small GTPases
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G51290 AT3G51290 AT3G51290 |
| fragaria_vesca | FvH4_1g11480 FvH4_1g11480 |
| malus_domestica | MD15G1242800.v1.1 |
| prunus_persica | Prupe.7G171300_v2.0.a1 Prupe.7G171300_v2.0.a1 |
| pyrus_communis | pycom02g09980 pycom15g21400 |
| rosa_chinensis | RchiOBHm_Chr2g0099091 |
| rosa_laevigata | RLG00000016847 |
| rosa_multiflora | Rmu_sc0012098.1_g000004 |
| rosa_roxburghii | Rroxscaffold_2G00143450 |
| rosa_rugosa | Rorug02G0076600 |
| rosa_samantha | Rh2AG125700 Rh2BG129000 Rh2CG130000 Rh2DG130400 |
| rosa_wichuraiana | Rw2G009680 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 472 |
| AccB1I | GGYRCC | 3 cut(s) | 520, 1323, 1473 |
| AccII | CGCG | 2 cut(s) | 313, 473 |
| AclWI | GGATC | 4 cut(s) | 609, 1486, 1499, 1788 |
| AcoI | YGGCCR | 1 cut(s) | 661 |
| AcsI | RAATTY | 1 cut(s) | 1449 |
| AcuI | CTGAAG | 1 cut(s) | 1533 |
| AcvI | CACGTG | 2 cut(s) | 336, 396 |
| AcyI | GRCGYC | 1 cut(s) | 521 |
| AfaI | GTAC | 5 cut(s) | 68, 122, 754, 870, 1139 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 695, 1303, 1348 |
| AflIII | ACRYGT | 2 cut(s) | 1455, 1831 |
| AgsI | TTSAA | 5 cut(s) | 168, 293, 988, 1559, 1860 |
| AjiI | CACGTC | 1 cut(s) | 1269 |
| AjnI | CCWGG | 4 cut(s) | 587, 694, 927, 1341 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 760, 792 |
| Alw21I | GWGCWC | 1 cut(s) | 1614 |
| Alw26I | GTCTC | 5 cut(s) | 22, 323, 435, 941, 1088 |
| AlwI | GGATC | 4 cut(s) | 609, 1486, 1499, 1788 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 149, 1598, 1709, 1921 |
| Ama87I | CYCGRG | 3 cut(s) | 1301, 1613, 1735 |
| AoxI | GGCC | 3 cut(s) | 661, 1086, 1657 |
| ApeKI | GCWGC | 4 cut(s) | 149, 647, 876, 1010 |
| ApoI | RAATTY | 1 cut(s) | 1449 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 760, 792 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 923 |
| AspLEI | GCGC | 1 cut(s) | 523 |
| AspS9I | GGNCC | 3 cut(s) | 339, 409, 1658 |
| AsuC2I | CCSGG | 1 cut(s) | 502 |
| AsuHPI | GGTGA | 2 cut(s) | 451, 490 |
| AsuII | TTCGAA | 1 cut(s) | 548 |
| AsuNHI | GCTAGC | 1 cut(s) | 563 |
| AvaI | CYCGRG | 3 cut(s) | 1301, 1613, 1735 |
| AvaII | GGWCC | 2 cut(s) | 339, 409 |
| BalI | TGGCCA | 1 cut(s) | 663 |
| BamHI | GGATCC | 1 cut(s) | 1491 |
| BanI | GGYRCC | 3 cut(s) | 520, 1323, 1473 |
| BanII | GRGCYC | 4 cut(s) | 320, 1266, 1354, 1614 |
| BbrPI | CACGTG | 2 cut(s) | 336, 396 |
| BbsI | GAAGAC | 2 cut(s) | 1031, 1040 |
| Bbv12I | GWGCWC | 1 cut(s) | 1614 |
| BbvI | GCAGC | 4 cut(s) | 136, 634, 888, 1022 |
| BccI | CCATC | 9 cut(s) | 565, 672, 766, 854, 1066, 1261, 1377, 1813, 1840 |
| BceAI | ACGGC | 1 cut(s) | 370 |
| BciT130I | CCWGG | 4 cut(s) | 589, 696, 929, 1343 |
| BclI | TGATCA | 1 cut(s) | 1912 |
| BcnI | CCSGG | 1 cut(s) | 502 |
| BcoDI | GTCTC | 5 cut(s) | 22, 323, 435, 941, 1088 |
| BfaI | CTAG | 3 cut(s) | 564, 1673, 1683 |
| BfmI | CTRYAG | 1 cut(s) | 1536 |
| BfoI | RGCGCY | 1 cut(s) | 524 |
| BlpI | GCTNAGC | 1 cut(s) | 1394 |
| BmcAI | AGTACT | 1 cut(s) | 754 |
| Bme1390I | CCNGG | 5 cut(s) | 502, 589, 696, 929, 1343 |
| Bme18I | GGWCC | 2 cut(s) | 339, 409 |
| BmeT110I | CYCGRG | 3 cut(s) | 1301, 1613, 1735 |
| BmgBI | CACGTC | 1 cut(s) | 1269 |
| BmgT120I | GGNCC | 3 cut(s) | 339, 409, 1658 |
| BmiI | GGNNCC | 8 cut(s) | 317, 410, 411, 522, 1325, 1388, 1475, 1493 |
| BmrFI | CCNGG | 5 cut(s) | 502, 589, 696, 929, 1343 |
| BmrI | ACTGGG | 1 cut(s) | 1697 |
| BmsI | GCATC | 2 cut(s) | 643, 1517 |
| BmtI | GCTAGC | 1 cut(s) | 567 |
| BmuI | ACTGGG | 1 cut(s) | 1697 |
| BpiI | GAAGAC | 2 cut(s) | 1031, 1040 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 551, 583 |
| BpmI | CTGGAG | 1 cut(s) | 1280 |
| Bpu10I | CCTNAGC | 1 cut(s) | 597 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1394 |
| Bpu14I | TTCGAA | 1 cut(s) | 548 |
| BpuEI | CTTGAG | 2 cut(s) | 1172, 1664 |
| BpuMI | CCSGG | 1 cut(s) | 502 |
| BsaAI | YACGTR | 2 cut(s) | 336, 396 |
| BsaBI | GATNNNNATC | 1 cut(s) | 52 |
| BsaHI | GRCGYC | 1 cut(s) | 521 |
| BsaI | GGTCTC | 1 cut(s) | 323 |
| BsaJI | CCNNGG | 5 cut(s) | 311, 444, 501, 516, 736 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 117, 147, 711, 741 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 695, 1303, 1348 |
| Bse118I | RCCGGY | 1 cut(s) | 421 |
| Bse1I | ACTGG | 5 cut(s) | 223, 1327, 1369, 1405, 1703 |
| Bse3DI | GCAATG | 2 cut(s) | 582, 1325 |
| Bse8I | GATNNNNATC | 1 cut(s) | 52 |
| BseBI | CCWGG | 4 cut(s) | 589, 696, 929, 1343 |
| BseDI | CCNNGG | 5 cut(s) | 311, 444, 501, 516, 736 |
| BseGI | GGATG | 2 cut(s) | 1388, 1930 |
| BseJI | GATNNNNATC | 1 cut(s) | 52 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 695, 1303, 1348 |
| BseMI | GCAATG | 2 cut(s) | 582, 1325 |
| BseMII | CTCAG | 6 cut(s) | 722, 1020, 1097, 1098, 1251, 1263 |
| BseNI | ACTGG | 5 cut(s) | 223, 1327, 1369, 1405, 1703 |
| BseRI | GAGGAG | 3 cut(s) | 297, 461, 1769 |
| BseXI | GCAGC | 4 cut(s) | 136, 634, 888, 1022 |
| Bsh1236I | CGCG | 2 cut(s) | 313, 473 |
| Bsh1285I | CGRYCG | 1 cut(s) | 437 |
| BshFI | GGCC | 3 cut(s) | 663, 1088, 1659 |
| BshNI | GGYRCC | 3 cut(s) | 520, 1323, 1473 |
| BsiEI | CGRYCG | 1 cut(s) | 437 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1614 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1301, 1613, 1735 |
| BsiSI | CCGG | 3 cut(s) | 422, 501, 1390 |
| BslFI | GGGAC | 5 cut(s) | 211, 422, 868, 1139, 1357 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 695, 1303, 1348 |
| BsmAI | GTCTC | 5 cut(s) | 22, 323, 435, 941, 1088 |
| BsmBI | CGTCTC | 1 cut(s) | 435 |
| BsmFI | GGGAC | 5 cut(s) | 211, 422, 868, 1139, 1357 |
| BsmI | GAATGC | 1 cut(s) | 598 |
| BsnI | GGCC | 3 cut(s) | 663, 1088, 1659 |
| Bso31I | GGTCTC | 1 cut(s) | 323 |
| BsoBI | CYCGRG | 3 cut(s) | 1301, 1613, 1735 |
| Bsp119I | TTCGAA | 1 cut(s) | 548 |
| Bsp1286I | GDGCHC | 4 cut(s) | 320, 1266, 1354, 1614 |
| Bsp143I | GATC | 8 cut(s) | 128, 601, 612, 1110, 1486, 1491, 1793, 1912 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1394 |
| Bsp19I | CCATGG | 1 cut(s) | 736 |
| BspANI | GGCC | 3 cut(s) | 663, 1088, 1659 |
| BspCNI | CTCAG | 6 cut(s) | 721, 1021, 1096, 1099, 1252, 1264 |
| BspFNI | CGCG | 2 cut(s) | 313, 473 |
| BspLI | GGNNCC | 8 cut(s) | 317, 410, 411, 522, 1325, 1388, 1475, 1493 |
| BspOI | GCTAGC | 1 cut(s) | 567 |
| BspPI | GGATC | 4 cut(s) | 609, 1486, 1499, 1788 |
| BspQI | GCTCTTC | 1 cut(s) | 1754 |
| BspT104I | TTCGAA | 1 cut(s) | 548 |
| BspT107I | GGYRCC | 3 cut(s) | 520, 1323, 1473 |
| BspTNI | GGTCTC | 1 cut(s) | 323 |
| BsrDI | GCAATG | 2 cut(s) | 582, 1325 |
| BsrFI | RCCGGY | 1 cut(s) | 421 |
| BsrI | ACTGG | 5 cut(s) | 223, 1327, 1369, 1405, 1703 |
| BssAI | RCCGGY | 1 cut(s) | 421 |
| BssECI | CCNNGG | 5 cut(s) | 311, 444, 501, 516, 736 |
| BssMI | GATC | 8 cut(s) | 128, 601, 612, 1110, 1486, 1491, 1793, 1912 |
| BssNI | GRCGYC | 1 cut(s) | 521 |
| BssT1I | CCWWGG | 1 cut(s) | 736 |
| Bst2UI | CCWGG | 4 cut(s) | 589, 696, 929, 1343 |
| Bst4CI | ACNGT | 5 cut(s) | 496, 517, 557, 892, 1537 |
| Bst6I | CTCTTC | 5 cut(s) | 965, 998, 1406, 1746, 1754 |
| BstACI | GRCGYC | 1 cut(s) | 521 |
| BstBAI | YACGTR | 2 cut(s) | 336, 396 |
| BstBI | TTCGAA | 1 cut(s) | 548 |
| BstC8I | GCNNGC | 7 cut(s) | 423, 511, 565, 1545, 1719, 1766, 1875 |
| BstDEI | CTNAG | 9 cut(s) | 597, 708, 1029, 1083, 1107, 1260, 1272, 1394, 1634 |
| BstDSI | CCRYGG | 3 cut(s) | 311, 516, 736 |
| BstEII | GGTNACC | 1 cut(s) | 439 |
| BstF5I | GGATG | 2 cut(s) | 1388, 1930 |
| BstFNI | CGCG | 2 cut(s) | 313, 473 |
| BstH2I | RGCGCY | 1 cut(s) | 524 |
| BstHHI | GCGC | 1 cut(s) | 523 |
| BstKTI | GATC | 8 cut(s) | 131, 604, 615, 1113, 1489, 1494, 1796, 1915 |
| BstMAI | GTCTC | 5 cut(s) | 22, 323, 435, 941, 1088 |
| BstMBI | GATC | 8 cut(s) | 128, 601, 612, 1110, 1486, 1491, 1793, 1912 |
| BstMCI | CGRYCG | 1 cut(s) | 437 |
| BstNI | CCWGG | 4 cut(s) | 589, 696, 929, 1343 |
| BstNSI | RCATGY | 2 cut(s) | 1547, 1835 |
| BstPI | GGTNACC | 1 cut(s) | 439 |
| BstSCI | CCNGG | 5 cut(s) | 500, 587, 694, 927, 1341 |
| BstSFI | CTRYAG | 1 cut(s) | 1536 |
| BstUI | CGCG | 2 cut(s) | 313, 473 |
| BstV1I | GCAGC | 4 cut(s) | 136, 634, 888, 1022 |
| BstV2I | GAAGAC | 2 cut(s) | 1031, 1040 |
| BstX2I | RGATCY | 3 cut(s) | 601, 1491, 1793 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 765 |
| BstYI | RGATCY | 3 cut(s) | 601, 1491, 1793 |
| BsuRI | GGCC | 3 cut(s) | 663, 1088, 1659 |
| BtgI | CCRYGG | 3 cut(s) | 311, 516, 736 |
| BtrI | CACGTC | 1 cut(s) | 1269 |
| BtsCI | GGATG | 2 cut(s) | 1388, 1930 |
| BtsI | GCAGTG | 1 cut(s) | 850 |
| BtsIMutI | CAGTG | 2 cut(s) | 562, 850 |
| Cac8I | GCNNGC | 7 cut(s) | 423, 511, 565, 1545, 1719, 1766, 1875 |
| CaiI | CAGNNNCTG | 4 cut(s) | 149, 1598, 1709, 1921 |
| CfoI | GCGC | 1 cut(s) | 523 |
| Cfr10I | RCCGGY | 1 cut(s) | 421 |
| Cfr13I | GGNCC | 3 cut(s) | 339, 409, 1658 |
| Cfr42I | CCGCGG | 1 cut(s) | 314 |
| CseI | GACGC | 1 cut(s) | 462 |
| CsiI | ACCWGGT | 1 cut(s) | 1341 |
| Csp6I | GTAC | 5 cut(s) | 67, 121, 753, 869, 1138 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 498, 533 |
| CviQI | GTAC | 5 cut(s) | 67, 121, 753, 869, 1138 |
| DdeI | CTNAG | 9 cut(s) | 597, 708, 1029, 1083, 1107, 1260, 1272, 1394, 1634 |
| DinI | GGCGCC | 1 cut(s) | 522 |
| DpnI | GATC | 8 cut(s) | 130, 603, 614, 1112, 1488, 1493, 1795, 1914 |
| DpnII | GATC | 8 cut(s) | 128, 601, 612, 1110, 1486, 1491, 1793, 1912 |
| DraI | TTTAAA | 1 cut(s) | 1606 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 472 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 472 |
| EaeI | YGGCCR | 1 cut(s) | 661 |
| Eam1104I | CTCTTC | 5 cut(s) | 965, 998, 1406, 1746, 1754 |
| EarI | CTCTTC | 5 cut(s) | 965, 998, 1406, 1746, 1754 |
| EciI | GGCGGA | 2 cut(s) | 291, 363 |
| Ecl136II | GAGCTC | 1 cut(s) | 1612 |
| Eco130I | CCWWGG | 1 cut(s) | 736 |
| Eco24I | GRGCYC | 4 cut(s) | 320, 1266, 1354, 1614 |
| Eco31I | GGTCTC | 1 cut(s) | 323 |
| Eco47I | GGWCC | 2 cut(s) | 339, 409 |
| Eco53kI | GAGCTC | 1 cut(s) | 1612 |
| Eco57I | CTGAAG | 1 cut(s) | 1533 |
| Eco72I | CACGTG | 2 cut(s) | 336, 396 |
| Eco88I | CYCGRG | 3 cut(s) | 1301, 1613, 1735 |
| Eco91I | GGTNACC | 1 cut(s) | 439 |
| EcoICRI | GAGCTC | 1 cut(s) | 1612 |
| EcoO109I | RGGNCCY | 1 cut(s) | 409 |
| EcoO65I | GGTNACC | 1 cut(s) | 439 |
| EcoRII | CCWGG | 4 cut(s) | 587, 694, 927, 1341 |
| EcoT14I | CCWWGG | 1 cut(s) | 736 |
| EcoT38I | GRGCYC | 4 cut(s) | 320, 1266, 1354, 1614 |
| EgeI | GGCGCC | 1 cut(s) | 522 |
| EheI | GGCGCC | 1 cut(s) | 522 |
| ErhI | CCWWGG | 1 cut(s) | 736 |
| Esp3I | CGTCTC | 1 cut(s) | 435 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 82, 114 |
| FaqI | GGGAC | 5 cut(s) | 211, 422, 868, 1139, 1357 |
| FauI | CCCGC | 1 cut(s) | 649 |
| FbaI | TGATCA | 1 cut(s) | 1912 |
| FokI | GGATG | 2 cut(s) | 1395, 1937 |
| FriOI | GRGCYC | 4 cut(s) | 320, 1266, 1354, 1614 |
| FspBI | CTAG | 3 cut(s) | 564, 1673, 1683 |
| GlaI | GCGC | 1 cut(s) | 522 |
| GsuI | CTGGAG | 1 cut(s) | 1280 |
| HaeII | RGCGCY | 1 cut(s) | 524 |
| HaeIII | GGCC | 3 cut(s) | 663, 1088, 1659 |
| HapII | CCGG | 3 cut(s) | 422, 501, 1390 |
| HgaI | GACGC | 1 cut(s) | 462 |
| HhaI | GCGC | 1 cut(s) | 523 |
| Hin1I | GRCGYC | 1 cut(s) | 521 |
| Hin6I | GCGC | 1 cut(s) | 521 |
| HinP1I | GCGC | 1 cut(s) | 521 |
| HincII | GTYRAC | 2 cut(s) | 981, 1631 |
| HindII | GTYRAC | 2 cut(s) | 981, 1631 |
| HindIII | AAGCTT | 1 cut(s) | 96 |
| HpaII | CCGG | 3 cut(s) | 422, 501, 1390 |
| HphI | GGTGA | 2 cut(s) | 451, 490 |
| Hpy166II | GTNNAC | 4 cut(s) | 339, 393, 981, 1631 |
| Hpy188I | TCNGA | 8 cut(s) | 137, 328, 478, 711, 1273, 1491, 1513, 1594 |
| Hpy8I | GTNNAC | 4 cut(s) | 339, 393, 981, 1631 |
| Hpy99I | CGWCG | 2 cut(s) | 391, 478 |
| HpyAV | CCTTC | 3 cut(s) | 537, 544, 1508 |
| HpyCH4III | ACNGT | 5 cut(s) | 496, 517, 557, 892, 1537 |
| HpyCH4IV | ACGT | 4 cut(s) | 335, 395, 1268, 1457 |
| HpyCH4V | TGCA | 6 cut(s) | 1338, 1481, 1543, 1694, 1816, 1873 |
| HpyF3I | CTNAG | 9 cut(s) | 597, 708, 1029, 1083, 1107, 1260, 1272, 1394, 1634 |
| HpySE526I | ACGT | 4 cut(s) | 335, 395, 1268, 1457 |
| Hsp92I | GRCGYC | 1 cut(s) | 521 |
| HspAI | GCGC | 1 cut(s) | 521 |
| KasI | GGCGCC | 1 cut(s) | 520 |
| KflI | GGGWCCC | 1 cut(s) | 409 |
| KroI | GCCGGC | 1 cut(s) | 421 |
| KroNI | GCCGGC | 1 cut(s) | 423 |
| Ksp22I | TGATCA | 1 cut(s) | 1912 |
| KspI | CCGCGG | 1 cut(s) | 314 |
| Kzo9I | GATC | 8 cut(s) | 128, 601, 612, 1110, 1486, 1491, 1793, 1912 |
| LguI | GCTCTTC | 1 cut(s) | 1754 |
| LmnI | GCTCC | 6 cut(s) | 162, 315, 329, 622, 1392, 1918 |
| Lsp1109I | GCAGC | 4 cut(s) | 136, 634, 888, 1022 |
| LweI | GCATC | 2 cut(s) | 643, 1517 |
| MabI | ACCWGGT | 1 cut(s) | 1341 |
| MaeI | CTAG | 3 cut(s) | 564, 1673, 1683 |
| MaeII | ACGT | 4 cut(s) | 335, 395, 1268, 1457 |
| MaeIII | GTNAC | 9 cut(s) | 11, 439, 487, 496, 815, 1062, 1358, 1638, 1786 |
| MalI | GATC | 8 cut(s) | 130, 603, 614, 1112, 1488, 1493, 1795, 1914 |
| MboI | GATC | 8 cut(s) | 128, 601, 612, 1110, 1486, 1491, 1793, 1912 |
| MflI | RGATCY | 3 cut(s) | 601, 1491, 1793 |
| MhlI | GDGCHC | 4 cut(s) | 320, 1266, 1354, 1614 |
| MlsI | TGGCCA | 1 cut(s) | 663 |
| MluCI | AATT | 5 cut(s) | 161, 745, 849, 1449, 1942 |
| MluNI | TGGCCA | 1 cut(s) | 663 |
| Mly113I | GGCGCC | 1 cut(s) | 521 |
| MlyI | GAGTC | 6 cut(s) | 277, 790, 1070, 1655, 1850, 1892 |
| MmeI | TCCRAC | 2 cut(s) | 456, 1038 |
| Mox20I | TGGCCA | 1 cut(s) | 663 |
| MroNI | GCCGGC | 1 cut(s) | 421 |
| MroXI | GAANNNNTTC | 1 cut(s) | 923 |
| MscI | TGGCCA | 1 cut(s) | 663 |
| MseI | TTAA | 9 cut(s) | 618, 642, 714, 830, 1242, 1532, 1605, 1929, 1945 |
| MslI | CAYNNNNRTG | 3 cut(s) | 359, 763, 769 |
| Msp20I | TGGCCA | 1 cut(s) | 663 |
| MspA1I | CMGCKG | 1 cut(s) | 313 |
| MspI | CCGG | 3 cut(s) | 422, 501, 1390 |
| MspR9I | CCNGG | 5 cut(s) | 502, 589, 696, 929, 1343 |
| Mva1269I | GAATGC | 1 cut(s) | 598 |
| MvaI | CCWGG | 4 cut(s) | 589, 696, 929, 1343 |
| MvnI | CGCG | 2 cut(s) | 313, 473 |
| NaeI | GCCGGC | 1 cut(s) | 423 |
| NarI | GGCGCC | 1 cut(s) | 521 |
| NciI | CCSGG | 1 cut(s) | 502 |
| NcoI | CCATGG | 1 cut(s) | 736 |
| NdeII | GATC | 8 cut(s) | 128, 601, 612, 1110, 1486, 1491, 1793, 1912 |
| NgoMIV | GCCGGC | 1 cut(s) | 421 |
| NheI | GCTAGC | 1 cut(s) | 563 |
| NlaIV | GGNNCC | 8 cut(s) | 317, 410, 411, 522, 1325, 1388, 1475, 1493 |
| NmuCI | GTSAC | 7 cut(s) | 439, 496, 815, 1062, 1358, 1638, 1786 |
| NspI | RCATGY | 2 cut(s) | 1547, 1835 |
| NspV | TTCGAA | 1 cut(s) | 548 |
| PaeI | GCATGC | 1 cut(s) | 1547 |
| PaeR7I | CTCGAG | 3 cut(s) | 1301, 1613, 1735 |
| PciI | ACATGT | 1 cut(s) | 1831 |
| PciSI | GCTCTTC | 1 cut(s) | 1754 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 133, 470 |
| PctI | GAATGC | 1 cut(s) | 598 |
| PdiI | GCCGGC | 1 cut(s) | 423 |
| PdmI | GAANNNNTTC | 1 cut(s) | 923 |
| PfeI | GAWTC | 5 cut(s) | 1276, 1442, 1518, 1595, 1676 |
| PfoI | TCCNGGA | 3 cut(s) | 587, 694, 927 |
| PleI | GAGTC | 6 cut(s) | 277, 789, 1069, 1654, 1849, 1891 |
| PluTI | GGCGCC | 1 cut(s) | 524 |
| PmaCI | CACGTG | 2 cut(s) | 336, 396 |
| PmlI | CACGTG | 2 cut(s) | 336, 396 |
| PpsI | GAGTC | 6 cut(s) | 277, 789, 1069, 1654, 1849, 1891 |
| Ppu21I | YACGTR | 2 cut(s) | 336, 396 |
| PpuMI | RGGWCCY | 1 cut(s) | 409 |
| PscI | ACATGT | 1 cut(s) | 1831 |
| Psp124BI | GAGCTC | 1 cut(s) | 1614 |
| Psp5II | RGGWCCY | 1 cut(s) | 409 |
| Psp6I | CCWGG | 4 cut(s) | 587, 694, 927, 1341 |
| PspCI | CACGTG | 2 cut(s) | 336, 396 |
| PspEI | GGTNACC | 1 cut(s) | 439 |
| PspGI | CCWGG | 4 cut(s) | 587, 694, 927, 1341 |
| PspN4I | GGNNCC | 8 cut(s) | 317, 410, 411, 522, 1325, 1388, 1475, 1493 |
| PspPI | GGNCC | 3 cut(s) | 339, 409, 1658 |
| PspPPI | RGGWCCY | 1 cut(s) | 409 |
| PspXI | VCTCGAGB | 1 cut(s) | 1301 |
| PstNI | CAGNNNCTG | 4 cut(s) | 149, 1598, 1709, 1921 |
| PsuI | RGATCY | 3 cut(s) | 601, 1491, 1793 |
| RsaI | GTAC | 5 cut(s) | 68, 122, 754, 870, 1139 |
| RsaNI | GTAC | 5 cut(s) | 67, 121, 753, 869, 1138 |
| RseI | CAYNNNNRTG | 3 cut(s) | 359, 763, 769 |
| SacI | GAGCTC | 1 cut(s) | 1614 |
| SacII | CCGCGG | 1 cut(s) | 314 |
| SapI | GCTCTTC | 1 cut(s) | 1754 |
| SaqAI | TTAA | 9 cut(s) | 618, 642, 714, 830, 1242, 1532, 1605, 1929, 1945 |
| Sau3AI | GATC | 8 cut(s) | 128, 601, 612, 1110, 1486, 1491, 1793, 1912 |
| Sau96I | GGNCC | 3 cut(s) | 339, 409, 1658 |
| ScaI | AGTACT | 1 cut(s) | 754 |
| SchI | GAGTC | 6 cut(s) | 277, 790, 1070, 1655, 1850, 1892 |
| ScrFI | CCNGG | 5 cut(s) | 502, 589, 696, 929, 1343 |
| SduI | GDGCHC | 4 cut(s) | 320, 1266, 1354, 1614 |
| SexAI | ACCWGGT | 1 cut(s) | 1341 |
| SfaNI | GCATC | 2 cut(s) | 643, 1517 |
| SfcI | CTRYAG | 1 cut(s) | 1536 |
| SfoI | GGCGCC | 1 cut(s) | 522 |
| Sfr274I | CTCGAG | 3 cut(s) | 1301, 1613, 1735 |
| Sfr303I | CCGCGG | 1 cut(s) | 314 |
| SfuI | TTCGAA | 1 cut(s) | 548 |
| SgrBI | CCGCGG | 1 cut(s) | 314 |
| SinI | GGWCC | 2 cut(s) | 339, 409 |
| SlaI | CTCGAG | 3 cut(s) | 1301, 1613, 1735 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 359, 763, 769 |
| SmlI | CTYRAG | 5 cut(s) | 1151, 1301, 1613, 1643, 1735 |
| SmoI | CTYRAG | 5 cut(s) | 1151, 1301, 1613, 1643, 1735 |
| SphI | GCATGC | 1 cut(s) | 1547 |
| Sse9I | AATT | 5 cut(s) | 161, 745, 849, 1449, 1942 |
| SspDI | GGCGCC | 1 cut(s) | 520 |
| SspMI | CTAG | 3 cut(s) | 564, 1673, 1683 |
| SstI | GAGCTC | 1 cut(s) | 1614 |
| StyD4I | CCNGG | 5 cut(s) | 500, 587, 694, 927, 1341 |
| StyI | CCWWGG | 1 cut(s) | 736 |
| TaaI | ACNGT | 5 cut(s) | 496, 517, 557, 892, 1537 |
| TaiI | ACGT | 4 cut(s) | 338, 398, 1271, 1460 |
| TaqII | GACCGA | 2 cut(s) | 423, 458 |
| TasI | AATT | 5 cut(s) | 161, 745, 849, 1449, 1942 |
| TatI | WGTACW | 1 cut(s) | 752 |
| TfiI | GAWTC | 5 cut(s) | 1276, 1442, 1518, 1595, 1676 |
| Tru1I | TTAA | 9 cut(s) | 618, 642, 714, 830, 1242, 1532, 1605, 1929, 1945 |
| Tru9I | TTAA | 9 cut(s) | 618, 642, 714, 830, 1242, 1532, 1605, 1929, 1945 |
| TscAI | CASTG | 2 cut(s) | 562, 850 |
| TseFI | GTSAC | 7 cut(s) | 439, 496, 815, 1062, 1358, 1638, 1786 |
| TseI | GCWGC | 4 cut(s) | 149, 647, 876, 1010 |
| Tsp45I | GTSAC | 7 cut(s) | 439, 496, 815, 1062, 1358, 1638, 1786 |
| TspDTI | ATGAA | 4 cut(s) | 86, 1016, 1298, 1785 |
| TspRI | CASTG | 2 cut(s) | 562, 850 |
| VpaK11BI | GGWCC | 2 cut(s) | 339, 409 |
| XapI | RAATTY | 1 cut(s) | 1449 |
| XceI | RCATGY | 2 cut(s) | 1547, 1835 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1045 |
| XhoI | CTCGAG | 3 cut(s) | 1301, 1613, 1735 |
| XmnI | GAANNNNTTC | 1 cut(s) | 923 |
| XspI | CTAG | 3 cut(s) | 564, 1673, 1683 |
| ZrmI | AGTACT | 1 cut(s) | 754 |