pycom15g21660
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005885
Physical Location & Seq
Reverse (-)
15705840 .. 15708211
2372 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1245 bp
ATGACTGACGACACCTCCGATAACTACCGGGTCGAACCGGTCGACCCGGTCCTCGTCCGTTATCATCCCTCGGAGCTCCAAATCGCCTCGGAGTTTCTCTCCACCTGGCTCCCCTTTCTCACCAGAGATCTCTGCCAGCACTGTTCCGCCAAACTCACCGATCGCGTCCGCTCTCTCGGCCCAGAACTTGGTGGCGATTCCATACCTGTTAACCCGGATGAGAATTCGAGTGATTTGAAACCGGAGAGCAAGGAGTTGCAGGTGGGTAGTGATAATGATATTGGTGAAGATAATCATGATGATGACACAAGTTCACTAGGTTGGAAAGACGGAGCTCAGGGGCTATCGGAGCCCGACCCTGAAGCTTCTAAAAGTGGATTGTGTCCTGAATCCCCCTTATTAGAGACTCCAATCCCTCGGATGTCATGGGCCGATATGGCACAGGAAGATGAGCTGGAGGAGGAGGAAGAGGAGGAAGAGGAAGAGTGCGAATTGAATAAGCGGGTGGTGAATGTGAATGACACGACTGGAGAGTTGAGGATTACAAAGTCTACATTGTCCAGGGAGCAGAGGGAACACATTAGGTTCATGAATGTTAAGAGGAAGAAAGATTATATTTGTTTGGAGAGGGTTAATGGGAAATTGGTTAACATTCTCGATGGGCTTGAGCTCCACACCGGTATCTTCAGTGCAGCGGAACAGAAGAGGATTGTTGATTATGTTTATGAGCTTCAAGAGATGGGCCGGAAGGGTGAATTAAAAGCACGTACGTATACAGCACCTCAAAAGTGGATGAGGGGCAAGGGACGAGTAACCATTCAGTTCGGCTGTTGCTACAATTATGCAGTGGATAAGTATGGTAATCCACCTGGTATCCTCCGCGATGATGTTGTTGATCCCATACCTAATCTTTTCAAGGTGATCATTAGGAGGCTGGTAAAATGGCATGTGCTTCCCCCGACCTGTGTACCTGATAGTTGCATCGTCAATGTATATGATGAAGGGGACTGTATACCTCCACATGTTGACAACCATGATTTTGTTCGACCTTTCTGCACCGTGTCCTTTCTTAGTGAGTGCAACATAGTTTTTGGGTCAAACTTAAAGATCATTGGTCCTGGCGAGTTTGAAGGTTCATTTGCCATTCCCTTGCCAGTTGGATCTGTTCTGGTTTTAAACGGAAGGGGGGCTGATGTAGCTAAGCATTGTGTGCCTGCATTACCTGCTCGTTATTGTATATCGTAG

Protein Analysis

415

Amino Acids

46.42

Weight (kDa)

4.77

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 225 - 405 1e-10 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 250
Acc36I ACCTGC 2 cut(s) 250, 1231
AccB7I CCANNNNNTGG 1 cut(s) 188
AccBSI CCGCTC 1 cut(s) 171
AccI GTMKAC 4 cut(s) 42, 551, 773, 1012
AccII CGCG 2 cut(s) 165, 882
AciI CCGC 5 cut(s) 147, 169, 502, 695, 880
AclWI GGATC 2 cut(s) 890, 1168
AcsI RAATTY 1 cut(s) 223
AcuI CTGAAG 2 cut(s) 381, 670
AfaI GTAC 2 cut(s) 769, 969
AfiI CCNNNNNNNGG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 1021
AgeI ACCGGT 2 cut(s) 37, 677
AgsI TTSAA 5 cut(s) 238, 496, 734, 916, 1130
AjnI CCWGG 4 cut(s) 104, 560, 868, 1117
AluBI AGCT 7 cut(s) 76, 335, 365, 454, 670, 730, 1199
AluI AGCT 7 cut(s) 76, 335, 365, 454, 670, 730, 1199
Alw21I GWGCWC 3 cut(s) 78, 337, 672
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 2 cut(s) 890, 1168
AoxI GGCC 3 cut(s) 178, 429, 742
ApeKI GCWGC 1 cut(s) 692
ApoI RAATTY 1 cut(s) 223
AsiGI ACCGGT 2 cut(s) 37, 677
AspS9I GGNCC 5 cut(s) 49, 179, 429, 742, 1115
AsuC2I CCSGG 3 cut(s) 29, 47, 215
AsuHPI GGTGA 6 cut(s) 112, 148, 296, 520, 764, 931
AvaII GGWCC 2 cut(s) 49, 1115
BaeI ACNNNNGTAYC 4 cut(s) 664, 697, 951, 984
BanII GRGCYC 4 cut(s) 78, 337, 354, 672
Bbv12I GWGCWC 3 cut(s) 78, 337, 672
BbvI GCAGC 1 cut(s) 704
BccI CCATC 2 cut(s) 653, 733
BcgI CGANNNNNNTGC 2 cut(s) 1035, 1069
BciT130I CCWGG 4 cut(s) 106, 562, 870, 1119
BciVI GTATCC 1 cut(s) 884
BclI TGATCA 1 cut(s) 921
BcnI CCSGG 3 cut(s) 29, 47, 215
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 1 cut(s) 317
BfuAI ACCTGC 2 cut(s) 250, 1231
BfuI GTATCC 1 cut(s) 884
BglI GCCNNNNNGGC 1 cut(s) 437
BglII AGATCT 1 cut(s) 127
BisI GCNGC 1 cut(s) 693
BlpI GCTNAGC 1 cut(s) 1200
BlsI GCNGC 1 cut(s) 694
Bme1390I CCNGG 7 cut(s) 29, 47, 106, 215, 562, 870, 1119
Bme18I GGWCC 2 cut(s) 49, 1115
BmgT120I GGNCC 5 cut(s) 49, 179, 429, 742, 1115
BmiI GGNNCC 2 cut(s) 110, 351
BmrFI CCNGG 7 cut(s) 29, 47, 106, 215, 562, 870, 1119
BmsI GCATC 1 cut(s) 990
BplI GAGNNNNNCTC 2 cut(s) 83, 115
BpmI CTGGAG 2 cut(s) 476, 549
Bpu10I CCTNAGC 1 cut(s) 336
Bpu1102I GCTNAGC 1 cut(s) 1200
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 3 cut(s) 29, 47, 215
BsaAI YACGTR 2 cut(s) 767, 771
BsaJI CCNNGG 4 cut(s) 69, 87, 416, 561
BsaWI WCCGGW 3 cut(s) 37, 241, 677
BsaXI ACNNNNNCTCC 1 cut(s) 29
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse118I RCCGGY 2 cut(s) 37, 677
Bse1I ACTGG 2 cut(s) 532, 1154
BseBI CCWGG 4 cut(s) 106, 562, 870, 1119
BseDI CCNNGG 4 cut(s) 69, 87, 416, 561
BseGI GGATG 4 cut(s) 64, 223, 426, 798
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMII CTCAG 1 cut(s) 350
BseNI ACTGG 2 cut(s) 532, 1154
BseRI GAGGAG 3 cut(s) 473, 476, 485
BseXI GCAGC 1 cut(s) 704
BsgI GTGCAG 2 cut(s) 711, 1039
Bsh1236I CGCG 2 cut(s) 165, 882
Bsh1285I CGRYCG 2 cut(s) 42, 163
BshFI GGCC 3 cut(s) 180, 431, 744
BshTI ACCGGT 2 cut(s) 37, 677
BsiEI CGRYCG 2 cut(s) 42, 163
BsiHKAI GWGCWC 3 cut(s) 78, 337, 672
BsiSI CCGG 7 cut(s) 28, 38, 47, 215, 242, 678, 745
BsiWI CGTACG 1 cut(s) 767
BslFI GGGAC 2 cut(s) 819, 1019
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 2 cut(s) 819, 1019
BsnI GGCC 3 cut(s) 180, 431, 744
Bsp1286I GDGCHC 4 cut(s) 78, 337, 354, 672
Bsp143I GATC 6 cut(s) 127, 160, 895, 921, 1107, 1160
Bsp1720I GCTNAGC 1 cut(s) 1200
BspACI CCGC 5 cut(s) 147, 169, 502, 695, 880
BspANI GGCC 3 cut(s) 180, 431, 744
BspCNI CTCAG 1 cut(s) 349
BspFNI CGCG 2 cut(s) 165, 882
BspHI TCATGA 2 cut(s) 295, 588
BspLI GGNNCC 2 cut(s) 110, 351
BspMI ACCTGC 2 cut(s) 250, 1231
BspPI GGATC 2 cut(s) 890, 1168
BsrBI CCGCTC 1 cut(s) 171
BsrFI RCCGGY 2 cut(s) 37, 677
BsrI ACTGG 2 cut(s) 532, 1154
BssAI RCCGGY 2 cut(s) 37, 677
BssECI CCNNGG 4 cut(s) 69, 87, 416, 561
BssMI GATC 6 cut(s) 127, 160, 895, 921, 1107, 1160
BssNAI GTATAC 2 cut(s) 774, 1013
Bst1107I GTATAC 2 cut(s) 774, 1013
Bst2UI CCWGG 4 cut(s) 106, 562, 870, 1119
Bst4CI ACNGT 3 cut(s) 143, 1010, 1060
Bst6I CTCTTC 4 cut(s) 462, 471, 477, 698
BstAPI GCANNNNNTGC 2 cut(s) 1210, 1223
BstBAI YACGTR 2 cut(s) 767, 771
BstC8I GCNNGC 2 cut(s) 137, 1215
BstDEI CTNAG 3 cut(s) 336, 1070, 1200
BstF5I GGATG 4 cut(s) 64, 223, 426, 798
BstFNI CGCG 2 cut(s) 165, 882
BstKTI GATC 6 cut(s) 130, 163, 898, 924, 1110, 1163
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 6 cut(s) 127, 160, 895, 921, 1107, 1160
BstMCI CGRYCG 2 cut(s) 42, 163
BstMWI GCNNNNNNNGC 6 cut(s) 177, 349, 437, 1196, 1210, 1223
BstNI CCWGG 4 cut(s) 106, 562, 870, 1119
BstNSI RCATGY 2 cut(s) 950, 1025
BstSCI CCNGG 7 cut(s) 27, 45, 104, 213, 560, 868, 1117
BstSNI TACGTA 1 cut(s) 771
BstUI CGCG 2 cut(s) 165, 882
BstV1I GCAGC 1 cut(s) 704
BstX2I RGATCY 2 cut(s) 127, 1160
BstYI RGATCY 2 cut(s) 127, 1160
BstZ17I GTATAC 2 cut(s) 774, 1013
BsuI GTATCC 1 cut(s) 884
BsuRI GGCC 3 cut(s) 180, 431, 744
BtgZI GCGATG 1 cut(s) 897
BtsCI GGATG 4 cut(s) 64, 223, 426, 798
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 3 cut(s) 139, 694, 852
BveI ACCTGC 2 cut(s) 250, 1231
Cac8I GCNNGC 2 cut(s) 137, 1215
CciI TCATGA 2 cut(s) 295, 588
Cfr10I RCCGGY 2 cut(s) 37, 677
Cfr13I GGNCC 5 cut(s) 49, 179, 429, 742, 1115
CseI GACGC 1 cut(s) 154
CsiI ACCWGGT 1 cut(s) 868
Csp6I GTAC 2 cut(s) 768, 968
CspAI ACCGGT 2 cut(s) 37, 677
CviAII CATG 6 cut(s) 296, 426, 589, 947, 1022, 1034
CviQI GTAC 2 cut(s) 768, 968
DdeI CTNAG 3 cut(s) 336, 1070, 1200
DpnI GATC 6 cut(s) 129, 162, 897, 923, 1109, 1162
DpnII GATC 6 cut(s) 127, 160, 895, 921, 1107, 1160
DraI TTTAAA 1 cut(s) 1176
Eam1104I CTCTTC 4 cut(s) 462, 471, 477, 698
EarI CTCTTC 4 cut(s) 462, 471, 477, 698
EciI GGCGGA 1 cut(s) 136
Ecl136II GAGCTC 3 cut(s) 76, 335, 670
Eco105I TACGTA 1 cut(s) 771
Eco24I GRGCYC 4 cut(s) 78, 337, 354, 672
Eco47I GGWCC 2 cut(s) 49, 1115
Eco53kI GAGCTC 3 cut(s) 76, 335, 670
Eco57I CTGAAG 2 cut(s) 381, 670
EcoICRI GAGCTC 3 cut(s) 76, 335, 670
EcoRI GAATTC 1 cut(s) 223
EcoRII CCWGG 4 cut(s) 104, 560, 868, 1117
EcoT38I GRGCYC 4 cut(s) 78, 337, 354, 672
FaeI CATG 6 cut(s) 299, 429, 592, 950, 1025, 1037
FaqI GGGAC 2 cut(s) 819, 1019
FatI CATG 6 cut(s) 295, 425, 588, 946, 1021, 1033
FauI CCCGC 1 cut(s) 495
FbaI TGATCA 1 cut(s) 921
FblI GTMKAC 4 cut(s) 42, 551, 773, 1012
Fnu4HI GCNGC 1 cut(s) 693
FokI GGATG 4 cut(s) 51, 230, 433, 805
FriOI GRGCYC 4 cut(s) 78, 337, 354, 672
Fsp4HI GCNGC 1 cut(s) 693
FspBI CTAG 1 cut(s) 317
GluI GCNGC 1 cut(s) 693
GsuI CTGGAG 2 cut(s) 476, 549
HaeIII GGCC 3 cut(s) 180, 431, 744
HapII CCGG 7 cut(s) 28, 38, 47, 215, 242, 678, 745
HgaI GACGC 1 cut(s) 154
Hin1II CATG 6 cut(s) 299, 429, 592, 950, 1025, 1037
HincII GTYRAC 4 cut(s) 43, 211, 649, 1027
HindII GTYRAC 4 cut(s) 43, 211, 649, 1027
HindIII AAGCTT 1 cut(s) 363
HinfI GANTC 3 cut(s) 197, 389, 406
HpaI GTTAAC 2 cut(s) 211, 649
HpaII CCGG 7 cut(s) 28, 38, 47, 215, 242, 678, 745
HphI GGTGA 6 cut(s) 112, 148, 296, 520, 764, 931
Hpy166II GTNNAC 9 cut(s) 43, 211, 314, 552, 649, 774, 968, 1013, 1027
Hpy188I TCNGA 5 cut(s) 19, 73, 91, 349, 420
Hpy188III TCNNGA 5 cut(s) 296, 386, 589, 656, 734
Hpy8I GTNNAC 9 cut(s) 43, 211, 314, 552, 649, 774, 968, 1013, 1027
HpyAV CCTTC 4 cut(s) 742, 995, 1124, 1176
HpyCH4III ACNGT 3 cut(s) 143, 1010, 1060
HpyCH4IV ACGT 2 cut(s) 766, 770
HpyCH4V TGCA 7 cut(s) 259, 692, 845, 981, 1056, 1080, 1217
HpyF10VI GCNNNNNNNGC 6 cut(s) 177, 349, 437, 1196, 1210, 1223
HpyF3I CTNAG 3 cut(s) 336, 1070, 1200
HpySE526I ACGT 2 cut(s) 766, 770
Hsp92II CATG 6 cut(s) 299, 429, 592, 950, 1025, 1037
Ksp22I TGATCA 1 cut(s) 921
KspAI GTTAAC 2 cut(s) 211, 649
Kzo9I GATC 6 cut(s) 127, 160, 895, 921, 1107, 1160
LmnI GCTCC 7 cut(s) 73, 81, 114, 332, 349, 565, 675
Lsp1109I GCAGC 1 cut(s) 704
LweI GCATC 1 cut(s) 990
MabI ACCWGGT 1 cut(s) 868
MaeI CTAG 1 cut(s) 317
MaeII ACGT 2 cut(s) 766, 770
MaeIII GTNAC 1 cut(s) 811
MalI GATC 6 cut(s) 129, 162, 897, 923, 1109, 1162
MbiI CCGCTC 1 cut(s) 171
MboI GATC 6 cut(s) 127, 160, 895, 921, 1107, 1160
MboII GAAGA 8 cut(s) 299, 458, 479, 488, 494, 616, 676, 715
MflI RGATCY 2 cut(s) 127, 1160
MhlI GDGCHC 4 cut(s) 78, 337, 354, 672
MluCI AATT 5 cut(s) 223, 491, 641, 755, 838
MlyI GAGTC 1 cut(s) 400
MmeI TCCRAC 2 cut(s) 302, 1138
MseI TTAA 7 cut(s) 210, 597, 633, 648, 758, 1103, 1175
MslI CAYNNNNRTG 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 695
MspI CCGG 7 cut(s) 28, 38, 47, 215, 242, 678, 745
MspR9I CCNGG 7 cut(s) 29, 47, 106, 215, 562, 870, 1119
MvaI CCWGG 4 cut(s) 106, 562, 870, 1119
MvnI CGCG 2 cut(s) 165, 882
MwoI GCNNNNNNNGC 6 cut(s) 177, 349, 437, 1196, 1210, 1223
NciI CCSGG 3 cut(s) 29, 47, 215
NdeII GATC 6 cut(s) 127, 160, 895, 921, 1107, 1160
NlaIII CATG 6 cut(s) 299, 429, 592, 950, 1025, 1037
NlaIV GGNNCC 2 cut(s) 110, 351
NmeAIII GCCGAG 1 cut(s) 156
NspI RCATGY 2 cut(s) 950, 1025
PagI TCATGA 2 cut(s) 295, 588
PaqCI CACCTGC 1 cut(s) 250
PciI ACATGT 1 cut(s) 1021
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 39
PfeI GAWTC 2 cut(s) 197, 389
Pfl23II CGTACG 1 cut(s) 767
PflFI GACNNNGTC 1 cut(s) 47
PflMI CCANNNNNTGG 1 cut(s) 188
PinAI ACCGGT 2 cut(s) 37, 677
PkrI GCNGC 1 cut(s) 694
Ple19I CGATCG 1 cut(s) 163
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
Ppu21I YACGTR 2 cut(s) 767, 771
PscI ACATGT 1 cut(s) 1021
Psp124BI GAGCTC 3 cut(s) 78, 337, 672
Psp6I CCWGG 4 cut(s) 104, 560, 868, 1117
PspGI CCWGG 4 cut(s) 104, 560, 868, 1117
PspLI CGTACG 1 cut(s) 767
PspN4I GGNNCC 2 cut(s) 110, 351
PspPI GGNCC 5 cut(s) 49, 179, 429, 742, 1115
PsuI RGATCY 2 cut(s) 127, 1160
PsyI GACNNNGTC 1 cut(s) 47
PvuI CGATCG 1 cut(s) 163
RsaI GTAC 2 cut(s) 769, 969
RsaNI GTAC 2 cut(s) 768, 968
RseI CAYNNNNRTG 1 cut(s) 300
SacI GAGCTC 3 cut(s) 78, 337, 672
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 7 cut(s) 210, 597, 633, 648, 758, 1103, 1175
SatI GCNGC 1 cut(s) 693
Sau3AI GATC 6 cut(s) 127, 160, 895, 921, 1107, 1160
Sau96I GGNCC 5 cut(s) 49, 179, 429, 742, 1115
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 7 cut(s) 29, 47, 106, 215, 562, 870, 1119
SduI GDGCHC 4 cut(s) 78, 337, 354, 672
SexAI ACCWGGT 1 cut(s) 868
SfaNI GCATC 1 cut(s) 990
SinI GGWCC 2 cut(s) 49, 1115
SmiMI CAYNNNNRTG 1 cut(s) 300
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
SnaBI TACGTA 1 cut(s) 771
Sse9I AATT 5 cut(s) 223, 491, 641, 755, 838
SsiI CCGC 5 cut(s) 147, 169, 502, 695, 880
SspMI CTAG 1 cut(s) 317
SstI GAGCTC 3 cut(s) 78, 337, 672
StyD4I CCNGG 7 cut(s) 27, 45, 104, 213, 560, 868, 1117
TaaI ACNGT 3 cut(s) 143, 1010, 1060
TaiI ACGT 2 cut(s) 769, 773
TaqI TCGA 5 cut(s) 33, 42, 227, 657, 1045
TasI AATT 5 cut(s) 223, 491, 641, 755, 838
TfiI GAWTC 2 cut(s) 197, 389
Tru1I TTAA 7 cut(s) 210, 597, 633, 648, 758, 1103, 1175
Tru9I TTAA 7 cut(s) 210, 597, 633, 648, 758, 1103, 1175
TscAI CASTG 3 cut(s) 146, 694, 852
TseI GCWGC 1 cut(s) 692
TspDTI ATGAA 4 cut(s) 577, 605, 1014, 1125
TspGWI ACGGA 3 cut(s) 47, 345, 1194
TspRI CASTG 3 cut(s) 146, 694, 852
Tth111I GACNNNGTC 1 cut(s) 47
Van91I CCANNNNNTGG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 49, 1115
XapI RAATTY 1 cut(s) 223
XceI RCATGY 2 cut(s) 950, 1025
XmiI GTMKAC 4 cut(s) 42, 551, 773, 1012
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.