pycom15g21950

Transmembrane and coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008389
Physical Location & Seq
Forward (+)
15976532 .. 15980589
4058 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2142 bp
ATGGCCCCGCCGTCGTATCTGACGCCGACGCAGCGGTACGCCGCCGGAGCTCTGTTCGCGTTCGCTCTTCACCAGGCTCAGATCCACCAGACGCGCCCTCTGCGATTGTCATCACAAGAAGAAGAGTCGACGGAGGAGCGGACCAGCAGCGTCAGCAGCGCCGACTCCGTCTCCGACGACCCGGATCTCTGGGTCCACGAAAACTCGGATCTCCTCGCACCCGTTTTCAGGTTTCTGGAGATTGATTCTGCAGCATGGTCTGGACTTCAGGAGAGTGCTGCTACTTCTCCTGCTAGCCATCATGTTGGAGCTTTCTTGAGGTTGCTTGCTGAAGAAGGTGGTCGTGATGGTAAGGATGCTTCCGAAAATTCAGATCAAGAGCTTGCGTTGTCAAAAGGTATTGACGCTATGGCGTCTAGCATGGAAAAGGATCATGAGTCTTCCCAGTCGAAGATGGAAAAGCATCGTGAATATGAAAATGAATGCCGCGAGAAGTTCTCCACAACTGAGGTGAAAGAGAACATTGAGGCGGCGGATGGGAAGTTGGGAACTTCACAGGAGAAAGGGTCCACAGCTGCGGTGAAGCACAAAAGGGAAGAAGTGGATGAGCATTTGGAGAAGCTGATGGAGAAATGCTCCACAGCTGATGTGAAACAAAAAATTGAAGAAGTGGACTCGCAATTGGAAAATCTGCATGGGACTGGTGGTAGTCTGGTTAGGGTTAAAGATGCAGTTGCGTCTACCAGTGACATTCGTGAGAAAACTATTGGAGAGTTGACAATGCTCAGTTATGAGAGGAAAGTTACAGTTCTCTATGAACTTCTTACAGCTTGTCTGGCTGATAAACGTGAAGAGAAGAAGAAATGTACCCGTCGGAGAAAGGGTTACGATGCTCGGCATCGTGTAGCTCTGCGTTTGCTAGCAACTTGGCTTGACGTCAAATGGATAAAAATGGAAGCCATTGAGACAATGGTTGCTTGCTCTGCAATGGCATTAGTAAAACAAGAAGAAGAAAAAGAAGGAACCCAATCACCAAAAGACAAATGGGCTAAATGGAAGCGTGGTGGTATCATTGGTGCTGCTGCAATAACTGGAGGAGCGTTGTTGACAATAACTGGTGGACTAGCAGCCCCCGCAATTGCTGCAGGACTTGGTGCTCTAGCTCCAACATTGGGCACTATCATCCCAGTCATTGGAGCAAGTGGGTTTGCCGCAGCTGCAACTGCTGCAGGATCTGTTGCTGGTTCCGTTGTTGTTGCTGCATCATTTGGAGCTGCTGGAGCTGGACTCACAGGGAGCAAAATGGCTAGAAGAACTGGAGGTGTCGATCAATTTGAGTTCAAAGCTATTGGAGAAAATCATAACCAGGGCAGGCTAGCAGTCGAGATCTTGGTTTCAGGATTTGTGTTTGATGAGGAAGACTTTACAAAGCCTTGGGAGGGACATGATGACAATTTGGAAAGGTATGCAGTGCTCTGGGAGTCTAAGAATTTAATTGCAGTGAGCACTGCAATTCAGGATTGGCTTACCTCAACAATTGCAATGGGTTTGATGAAGCAAGGTGCAATGATGACTGTGTTAGGCACTCTTGTAACTGCATTTGCTTGGCCGGCAACATTACTTGCGGCTACTGCTTTTATAGACAGCAAATGGGCAATTGCCGTGGACAGGTCAGACAAAGCAGGAAAACTTCTTGCTGAGGTGTTACTAAAGGGAATTCATGGGAACAGGCCTGTAACACTTGTGGGTTTCTCACTTGGAGCACGAGTAATTTTCAAGTGTCTCCAGTGTTTGGCAGAAACTGAACATCATGCTGAACTTGTAGAGCGAGTTGTTCTGCTTGGAGCACCCATCTCGATTGCAGATGAGAAATGGGAAGCTGCTAGAAAGATGGTGGCTGGAAGATTTGTGAATGCGTACTCCACAAATGATTGGATGCTTGGGGTTGCTTTCCGTGCTAGTCTACTTTCTCGAGGATTAGCTGGAATTCAACCAATCAATGTTCAAGGGATTGAGAATGTGGATGTAACGGACATAATTGATTCGCACTCTTCCTATCTTTGGTCTACAAAGCAGATCCTGGAGCTGGTTGAACTGGAAGGCTATTATCCCGTTCACAGGAGCATGATTTGCATAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF726

Protein Analysis

714

Amino Acids

77.26

Weight (kDa)

5.59

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF726 PF05277 351 - 699 1.5e-98 Protein of unknown function (DUF726)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 939
AccB7I CCANNNNNTGG 2 cut(s) 1193, 1792
AccBSI CCGCTC 1 cut(s) 139
AccI GTMKAC 4 cut(s) 128, 740, 1963, 2066
AccII CGCG 3 cut(s) 59, 94, 489
AclWI GGATC 6 cut(s) 76, 192, 216, 438, 1240, 2071
AcoI YGGCCR 1 cut(s) 1607
AcsI RAATTY 4 cut(s) 367, 1489, 1716, 1986
AcuI CTGAAG 2 cut(s) 251, 351
AcyI GRCGYC 3 cut(s) 23, 413, 936
AfaI GTAC 3 cut(s) 38, 868, 1919
AfiI CCNNNNNNNGG 9 cut(s) 228, 1172, 1193, 1439, 1668, 1792, 2061, 2086, 2118
AgsI TTSAA 6 cut(s) 665, 1342, 1777, 1991, 2006, 2093
AjnI CCWGG 3 cut(s) 72, 1365, 2079
Alw21I GWGCWC 6 cut(s) 52, 1159, 1476, 1508, 1765, 1849
Alw26I GTCTC 3 cut(s) 175, 959, 1787
AlwI GGATC 6 cut(s) 76, 192, 216, 438, 1240, 2071
AlwNI CAGNNNCTG 3 cut(s) 1235, 1802, 2080
Ama87I CYCGRG 1 cut(s) 1971
AoxI GGCC 3 cut(s) 3, 1607, 1730
ApoI RAATTY 4 cut(s) 367, 1489, 1716, 1986
AspLEI GCGC 2 cut(s) 96, 161
AspS9I GGNCC 4 cut(s) 4, 141, 193, 567
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 4 cut(s) 62, 523, 592, 1023
AsuNHI GCTAGC 3 cut(s) 293, 919, 1375
AvaI CYCGRG 1 cut(s) 1971
AvaII GGWCC 3 cut(s) 141, 193, 567
BaeGI GKGCMC 1 cut(s) 1178
BanII GRGCYC 1 cut(s) 52
BarI GAAGNNNNNNTAC 2 cut(s) 343, 375
BauI CACGAG 1 cut(s) 1764
BbsI GAAGAC 2 cut(s) 432, 1425
Bbv12I GWGCWC 6 cut(s) 52, 1159, 1476, 1508, 1765, 1849
BbvCI CCTCAGC 1 cut(s) 1698
BccI CCATC 7 cut(s) 306, 341, 448, 530, 619, 1859, 1885
BceAI ACGGC 1 cut(s) 1646
BcgI CGANNNNNNTGC 2 cut(s) 1836, 1870
BciT130I CCWGG 3 cut(s) 74, 1367, 2081
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 3 cut(s) 175, 959, 1787
BfaI CTAG 9 cut(s) 294, 417, 920, 1124, 1160, 1308, 1376, 1884, 1959
BfmI CTRYAG 3 cut(s) 249, 1143, 1227
BfoI RGCGCY 1 cut(s) 162
BglII AGATCT 1 cut(s) 1386
Bme1390I CCNGG 4 cut(s) 74, 182, 1367, 2081
Bme18I GGWCC 3 cut(s) 141, 193, 567
BmeT110I CYCGRG 1 cut(s) 1971
BmgT120I GGNCC 4 cut(s) 4, 141, 193, 567
BmiI GGNNCC 5 cut(s) 6, 194, 568, 1024, 1246
BmrFI CCNGG 4 cut(s) 74, 182, 1367, 2081
BmrI ACTGGG 2 cut(s) 439, 1181
BmsI GCATC 7 cut(s) 346, 472, 718, 880, 907, 1271, 1926
BmtI GCTAGC 3 cut(s) 297, 923, 1379
BmuI ACTGGG 2 cut(s) 439, 1181
BpiI GAAGAC 2 cut(s) 432, 1425
BplI GAGNNNNNCTC 6 cut(s) 482, 514, 620, 652, 1272, 1304
BpmI CTGGAG 6 cut(s) 257, 1113, 1299, 1338, 1769, 2102
Bpu10I CCTNAGC 1 cut(s) 1698
BpuEI CTTGAG 1 cut(s) 337
BpuMI CCSGG 1 cut(s) 182
BsaBI GATNNNNATC 1 cut(s) 109
BsaHI GRCGYC 3 cut(s) 23, 413, 936
BsaJI CCNNGG 3 cut(s) 1366, 1433, 1662
BsaXI ACNNNNNCTCC 8 cut(s) 155, 185, 551, 581, 868, 898, 1752, 1782
Bsc4I CCNNNNNNNGG 9 cut(s) 228, 1172, 1193, 1439, 1668, 1792, 2061, 2086, 2118
Bse118I RCCGGY 1 cut(s) 1609
Bse1I ACTGG 9 cut(s) 445, 706, 744, 1096, 1120, 1187, 1321, 1786, 2100
Bse3DI GCAATG 3 cut(s) 993, 1548, 1572
Bse8I GATNNNNATC 1 cut(s) 109
BseBI CCWGG 3 cut(s) 74, 1367, 2081
BseDI CCNNGG 3 cut(s) 1366, 1433, 1662
BseGI GGATG 6 cut(s) 361, 541, 610, 1182, 1941, 2029
BseJI GATNNNNATC 1 cut(s) 109
BseLI CCNNNNNNNGG 9 cut(s) 228, 1172, 1193, 1439, 1668, 1792, 2061, 2086, 2118
BseMI GCAATG 3 cut(s) 993, 1548, 1572
BseMII CTCAG 4 cut(s) 92, 498, 799, 1689
BseNI ACTGG 9 cut(s) 445, 706, 744, 1096, 1120, 1187, 1321, 1786, 2100
BseRI GAGGAG 3 cut(s) 149, 203, 1110
BseSI GKGCMC 1 cut(s) 1178
Bsh1236I CGCG 3 cut(s) 59, 94, 489
BshFI GGCC 3 cut(s) 5, 1609, 1732
BsiHKAI GWGCWC 6 cut(s) 52, 1159, 1476, 1508, 1765, 1849
BsiHKCI CYCGRG 1 cut(s) 1971
BsiSI CCGG 3 cut(s) 45, 182, 1610
BslFI GGGAC 2 cut(s) 712, 1455
BslI CCNNNNNNNGG 9 cut(s) 228, 1172, 1193, 1439, 1668, 1792, 2061, 2086, 2118
BsmAI GTCTC 3 cut(s) 175, 959, 1787
BsmBI CGTCTC 1 cut(s) 175
BsmFI GGGAC 2 cut(s) 712, 1455
BsmI GAATGC 2 cut(s) 488, 1918
BsnI GGCC 3 cut(s) 5, 1609, 1732
BsoBI CYCGRG 1 cut(s) 1971
Bsp1286I GDGCHC 7 cut(s) 52, 1159, 1178, 1476, 1508, 1765, 1849
Bsp143I GATC 9 cut(s) 81, 184, 208, 373, 430, 1232, 1327, 1386, 2076
BspANI GGCC 3 cut(s) 5, 1609, 1732
BspCNI CTCAG 4 cut(s) 91, 499, 798, 1690
BspFNI CGCG 3 cut(s) 59, 94, 489
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 5 cut(s) 6, 194, 568, 1024, 1246
BspMAI CTGCAG 3 cut(s) 253, 1147, 1231
BspOI GCTAGC 3 cut(s) 297, 923, 1379
BspPI GGATC 6 cut(s) 76, 192, 216, 438, 1240, 2071
BspQI GCTCTTC 1 cut(s) 72
BsrBI CCGCTC 1 cut(s) 139
BsrDI GCAATG 3 cut(s) 993, 1548, 1572
BsrFI RCCGGY 1 cut(s) 1609
BsrI ACTGG 9 cut(s) 445, 706, 744, 1096, 1120, 1187, 1321, 1786, 2100
BssAI RCCGGY 1 cut(s) 1609
BssECI CCNNGG 3 cut(s) 1366, 1433, 1662
BssMI GATC 9 cut(s) 81, 184, 208, 373, 430, 1232, 1327, 1386, 2076
BssNI GRCGYC 3 cut(s) 23, 413, 936
BssSI CACGAG 1 cut(s) 1764
BssT1I CCWWGG 1 cut(s) 1433
Bst2BI CACGAG 1 cut(s) 1764
Bst2UI CCWGG 3 cut(s) 74, 1367, 2081
Bst4CI ACNGT 2 cut(s) 808, 1576
Bst6I CTCTTC 4 cut(s) 72, 117, 846, 2056
BstACI GRCGYC 3 cut(s) 23, 413, 936
BstAPI GCANNNNNTGC 3 cut(s) 1142, 1226, 2130
BstC8I GCNNGC 8 cut(s) 295, 327, 384, 921, 979, 1373, 1377, 1611
BstDEI CTNAG 5 cut(s) 78, 507, 785, 1485, 1698
BstDSI CCRYGG 1 cut(s) 1662
BstF5I GGATG 6 cut(s) 361, 541, 610, 1182, 1941, 2029
BstFNI CGCG 3 cut(s) 59, 94, 489
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 2 cut(s) 96, 161
BstKTI GATC 9 cut(s) 84, 187, 211, 376, 433, 1235, 1330, 1389, 2079
BstMAI GTCTC 3 cut(s) 175, 959, 1787
BstMBI GATC 9 cut(s) 81, 184, 208, 373, 430, 1232, 1327, 1386, 2076
BstNI CCWGG 3 cut(s) 74, 1367, 2081
BstSCI CCNGG 4 cut(s) 72, 180, 1365, 2079
BstSFI CTRYAG 3 cut(s) 249, 1143, 1227
BstSLI GKGCMC 1 cut(s) 1178
BstUI CGCG 3 cut(s) 59, 94, 489
BstV2I GAAGAC 2 cut(s) 432, 1425
BstX2I RGATCY 6 cut(s) 81, 184, 208, 1232, 1386, 2076
BstXI CCANNNNNNTGG 1 cut(s) 305
BstYI RGATCY 6 cut(s) 81, 184, 208, 1232, 1386, 2076
BsuRI GGCC 3 cut(s) 5, 1609, 1732
BtgI CCRYGG 1 cut(s) 1662
BtsCI GGATG 6 cut(s) 361, 541, 610, 1182, 1941, 2029
BtsI GCAGTG 3 cut(s) 1476, 1506, 1506
BtsIMutI CAGTG 5 cut(s) 751, 1476, 1506, 1506, 1793
Cac8I GCNNGC 8 cut(s) 295, 327, 384, 921, 979, 1373, 1377, 1611
CaiI CAGNNNCTG 3 cut(s) 1235, 1802, 2080
CciI TCATGA 1 cut(s) 433
CfoI GCGC 2 cut(s) 96, 161
Cfr10I RCCGGY 1 cut(s) 1609
Cfr13I GGNCC 4 cut(s) 4, 141, 193, 567
CseI GACGC 7 cut(s) 31, 37, 100, 139, 402, 413, 726
Csp6I GTAC 3 cut(s) 37, 867, 1918
CspCI CAANNNNNGTGG 2 cut(s) 1644, 1679
CviAII CATG 9 cut(s) 255, 302, 421, 434, 695, 1445, 1721, 1811, 2125
CviQI GTAC 3 cut(s) 37, 867, 1918
DdeI CTNAG 5 cut(s) 78, 507, 785, 1485, 1698
DpnI GATC 9 cut(s) 83, 186, 210, 375, 432, 1234, 1329, 1388, 2078
DpnII GATC 9 cut(s) 81, 184, 208, 373, 430, 1232, 1327, 1386, 2076
EaeI YGGCCR 1 cut(s) 1607
Eam1104I CTCTTC 4 cut(s) 72, 117, 846, 2056
EarI CTCTTC 4 cut(s) 72, 117, 846, 2056
EciI GGCGGA 1 cut(s) 548
Ecl136II GAGCTC 1 cut(s) 50
Eco130I CCWWGG 1 cut(s) 1433
Eco147I AGGCCT 1 cut(s) 1732
Eco24I GRGCYC 1 cut(s) 52
Eco47I GGWCC 3 cut(s) 141, 193, 567
Eco53kI GAGCTC 1 cut(s) 50
Eco57I CTGAAG 2 cut(s) 251, 351
Eco88I CYCGRG 1 cut(s) 1971
EcoICRI GAGCTC 1 cut(s) 50
EcoRI GAATTC 2 cut(s) 1716, 1986
EcoRII CCWGG 3 cut(s) 72, 1365, 2079
EcoT14I CCWWGG 1 cut(s) 1433
EcoT38I GRGCYC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 1433
Esp3I CGTCTC 1 cut(s) 175
FaeI CATG 9 cut(s) 258, 305, 424, 437, 698, 1448, 1724, 1814, 2128
FaqI GGGAC 2 cut(s) 712, 1455
FatI CATG 9 cut(s) 254, 301, 420, 433, 694, 1444, 1720, 1810, 2124
FauI CCCGC 2 cut(s) 15, 1141
FblI GTMKAC 4 cut(s) 128, 740, 1963, 2066
FokI GGATG 6 cut(s) 368, 548, 617, 1169, 1948, 2036
FriOI GRGCYC 1 cut(s) 52
FspBI CTAG 9 cut(s) 294, 417, 920, 1124, 1160, 1308, 1376, 1884, 1959
GlaI GCGC 2 cut(s) 95, 160
GsuI CTGGAG 6 cut(s) 257, 1113, 1299, 1338, 1769, 2102
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 3 cut(s) 5, 1609, 1732
HapII CCGG 3 cut(s) 45, 182, 1610
HgaI GACGC 7 cut(s) 31, 37, 100, 139, 402, 413, 726
HhaI GCGC 2 cut(s) 96, 161
Hin1I GRCGYC 3 cut(s) 23, 413, 936
Hin1II CATG 9 cut(s) 258, 305, 424, 437, 698, 1448, 1724, 1814, 2128
Hin6I GCGC 2 cut(s) 94, 159
HinP1I GCGC 2 cut(s) 94, 159
HincII GTYRAC 3 cut(s) 129, 777, 1107
HindII GTYRAC 3 cut(s) 129, 777, 1107
HinfI GANTC 8 cut(s) 125, 164, 245, 437, 674, 1287, 1481, 2042
HpaII CCGG 3 cut(s) 45, 182, 1610
HphI GGTGA 4 cut(s) 62, 523, 592, 1023
Hpy188I TCNGA 8 cut(s) 21, 81, 175, 208, 364, 373, 876, 1675
Hpy99I CGWCG 5 cut(s) 16, 31, 133, 179, 876
HpyAV CCTTC 3 cut(s) 329, 1013, 2093
HpyCH4III ACNGT 2 cut(s) 808, 1576
HpyCH4IV ACGT 2 cut(s) 847, 936
HpyF3I CTNAG 5 cut(s) 78, 507, 785, 1485, 1698
HpySE526I ACGT 2 cut(s) 847, 936
Hsp92I GRCGYC 3 cut(s) 23, 413, 936
Hsp92II CATG 9 cut(s) 258, 305, 424, 437, 698, 1448, 1724, 1814, 2128
HspAI GCGC 2 cut(s) 94, 159
KroI GCCGGC 1 cut(s) 1609
KroNI GCCGGC 1 cut(s) 1611
Kzo9I GATC 9 cut(s) 81, 184, 208, 373, 430, 1232, 1327, 1386, 2076
LguI GCTCTTC 1 cut(s) 72
LweI GCATC 7 cut(s) 346, 472, 718, 880, 907, 1271, 1926
MaeI CTAG 9 cut(s) 294, 417, 920, 1124, 1160, 1308, 1376, 1884, 1959
MaeII ACGT 2 cut(s) 847, 936
MaeIII GTNAC 7 cut(s) 746, 802, 884, 1591, 1704, 1735, 2026
MalI GATC 9 cut(s) 83, 186, 210, 375, 432, 1234, 1329, 1388, 2078
MbiI CCGCTC 1 cut(s) 139
MboI GATC 9 cut(s) 81, 184, 208, 373, 430, 1232, 1327, 1386, 2076
MfeI CAATTG 4 cut(s) 680, 1137, 1536, 1656
MflI RGATCY 6 cut(s) 81, 184, 208, 1232, 1386, 2076
MhlI GDGCHC 7 cut(s) 52, 1159, 1178, 1476, 1508, 1765, 1849
MlyI GAGTC 6 cut(s) 134, 158, 446, 668, 1281, 1490
MmeI TCCRAC 4 cut(s) 198, 286, 854, 1190
MroNI GCCGGC 1 cut(s) 1609
MseI TTAA 2 cut(s) 723, 1493
MspA1I CMGCKG 4 cut(s) 34, 575, 644, 1217
MspI CCGG 3 cut(s) 45, 182, 1610
MspR9I CCNGG 4 cut(s) 74, 182, 1367, 2081
MunI CAATTG 4 cut(s) 680, 1137, 1536, 1656
Mva1269I GAATGC 2 cut(s) 488, 1918
MvaI CCWGG 3 cut(s) 74, 1367, 2081
MvnI CGCG 3 cut(s) 59, 94, 489
NaeI GCCGGC 1 cut(s) 1611
NciI CCSGG 1 cut(s) 182
NdeII GATC 9 cut(s) 81, 184, 208, 373, 430, 1232, 1327, 1386, 2076
NgoMIV GCCGGC 1 cut(s) 1609
NheI GCTAGC 3 cut(s) 293, 919, 1375
NlaIII CATG 9 cut(s) 258, 305, 424, 437, 698, 1448, 1724, 1814, 2128
NlaIV GGNNCC 5 cut(s) 6, 194, 568, 1024, 1246
NmeAIII GCCGAG 1 cut(s) 874
NmuCI GTSAC 1 cut(s) 746
PaeR7I CTCGAG 1 cut(s) 1971
PagI TCATGA 1 cut(s) 433
PceI AGGCCT 1 cut(s) 1732
PciSI GCTCTTC 1 cut(s) 72
PctI GAATGC 2 cut(s) 488, 1918
PdiI GCCGGC 1 cut(s) 1611
PfeI GAWTC 2 cut(s) 245, 2042
PflFI GACNNNGTC 1 cut(s) 167
PflMI CCANNNNNTGG 2 cut(s) 1193, 1792
PfoI TCCNGGA 1 cut(s) 2079
PleI GAGTC 6 cut(s) 133, 158, 445, 668, 1281, 1489
PpsI GAGTC 6 cut(s) 133, 158, 445, 668, 1281, 1489
Psp124BI GAGCTC 1 cut(s) 52
Psp6I CCWGG 3 cut(s) 72, 1365, 2079
PspGI CCWGG 3 cut(s) 72, 1365, 2079
PspN4I GGNNCC 5 cut(s) 6, 194, 568, 1024, 1246
PspPI GGNCC 4 cut(s) 4, 141, 193, 567
PstI CTGCAG 3 cut(s) 253, 1147, 1231
PstNI CAGNNNCTG 3 cut(s) 1235, 1802, 2080
PsuI RGATCY 6 cut(s) 81, 184, 208, 1232, 1386, 2076
PsyI GACNNNGTC 1 cut(s) 167
PvuII CAGCTG 3 cut(s) 575, 644, 1217
RsaI GTAC 3 cut(s) 38, 868, 1919
RsaNI GTAC 3 cut(s) 37, 867, 1918
SacI GAGCTC 1 cut(s) 52
SalI GTCGAC 1 cut(s) 127
SapI GCTCTTC 1 cut(s) 72
SaqAI TTAA 2 cut(s) 723, 1493
Sau3AI GATC 9 cut(s) 81, 184, 208, 373, 430, 1232, 1327, 1386, 2076
Sau96I GGNCC 4 cut(s) 4, 141, 193, 567
SchI GAGTC 6 cut(s) 134, 158, 446, 668, 1281, 1490
ScrFI CCNGG 4 cut(s) 74, 182, 1367, 2081
SduI GDGCHC 7 cut(s) 52, 1159, 1178, 1476, 1508, 1765, 1849
SfaNI GCATC 7 cut(s) 346, 472, 718, 880, 907, 1271, 1926
SfcI CTRYAG 3 cut(s) 249, 1143, 1227
Sfr274I CTCGAG 1 cut(s) 1971
SinI GGWCC 3 cut(s) 141, 193, 567
SlaI CTCGAG 1 cut(s) 1971
SmlI CTYRAG 2 cut(s) 316, 1971
SmoI CTYRAG 2 cut(s) 316, 1971
SseBI AGGCCT 1 cut(s) 1732
SspMI CTAG 9 cut(s) 294, 417, 920, 1124, 1160, 1308, 1376, 1884, 1959
SstI GAGCTC 1 cut(s) 52
StuI AGGCCT 1 cut(s) 1732
StyD4I CCNGG 4 cut(s) 72, 180, 1365, 2079
StyI CCWWGG 1 cut(s) 1433
TaaI ACNGT 2 cut(s) 808, 1576
TaiI ACGT 2 cut(s) 850, 939
TaqI TCGA 6 cut(s) 128, 449, 1326, 1383, 1856, 1972
TauI GCSGC 5 cut(s) 44, 489, 533, 1214, 1628
TfiI GAWTC 2 cut(s) 245, 2042
Tru1I TTAA 2 cut(s) 723, 1493
Tru9I TTAA 2 cut(s) 723, 1493
TscAI CASTG 5 cut(s) 751, 1476, 1506, 1513, 1793
TseFI GTSAC 1 cut(s) 746
Tsp45I GTSAC 1 cut(s) 746
TspDTI ATGAA 5 cut(s) 489, 495, 831, 1568, 1709
TspGWI ACGGA 5 cut(s) 146, 157, 1237, 1943, 2045
TspRI CASTG 5 cut(s) 751, 1476, 1506, 1513, 1793
Tth111I GACNNNGTC 1 cut(s) 167
Van91I CCANNNNNTGG 2 cut(s) 1193, 1792
VpaK11BI GGWCC 3 cut(s) 141, 193, 567
XapI RAATTY 4 cut(s) 367, 1489, 1716, 1986
XcmI CCANNNNNNNNNTGG 2 cut(s) 967, 1041
XhoI CTCGAG 1 cut(s) 1971
XmiI GTMKAC 4 cut(s) 128, 740, 1963, 2066
XspI CTAG 9 cut(s) 294, 417, 920, 1124, 1160, 1308, 1376, 1884, 1959
ZraI GACGTC 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.