Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0005351

Physical Location & Seq

Genomic Coordinates Forward (+)

23198993 .. 23206755

Gene Structure 7763 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3000 bp

ATGCCATGGCTAACGTGCACGAAGCTGACGCTTTTCACCATAGCTGGAGTCGCGATTCAAATGGTTGGCCTCTCCATCTTCGTCTTCGGCTTCTTCCCCGTCAAACCAACCCTTCCCGGACACAGTGGTTCGGAGAATTTTCGAGCACCGCAGTGCGATTTGGTACGAAATGAGAGTGAGAGTGATCTGCATCCCCATCAGCTCAGGTCACTATATAAGGTACATGTTTCTTTCATGCTATTTTTGGGTTGGCAGCTTTTACTTTCACTCACGTCTTGTTTCGAGAAATTCGTCAGTCTTGCTCTTAGTGTTATTGACGGTCTTCCAGCAGAACTCGTGCTTGGCAAGGATGGTAAGCCTCCAAGCAAGGCGTTAATGGAAGCTATGCCATATACTCAGTCACTGCTTGCAAATGGAATGGCAATTGGGTTCCATGCAAAGGCTGCACCTCCAACTGTTACAATGCCTCGTTTGAAGGCTATGGTTTCTGGAGCAATAGGGGGATTCCTGGACGTGGCTATGAATTTTAACACACAAGCTATGTTAGATGACAATCTTCTCGGCCAGTTTTTCAAGATTGGTTGGAAAATGGTGATGCTTGGTGATGAAACATGGCTTAAGTTGTTTCCCGGATTATTTTTGAGGCATGATGGAGTCAGCAGTTTCTTTGTTAAAGATACTGTACATGTGGATCAAAATGTTTCTCGACATCTGGGTTATGAACTTAGCAGAGATGACTGGGATTTTCTGATTCTCCACTATTTGGGCTTAGATCATGTTGGACATATTGGTGGGCGCAACAGTGCCTTGATGGCCCCAAAACTTCGAGAGATGGATGAGGTGGTGAAGATGATTCATGTGAATAGTATACTAAATCAAAAGAATGATGAAGGACAGACACTCTTGGTTGTAGTAAGTGACCATGGCATGACTGAGAGTGGTAATCACGGAGGGTCCTCCTATGAAGAAACTGACTCCTTAGCTCTTTTCATTGGCGTGAAAAATAACATCTCTGATGACTTCTTGTCCACCCATAACATTGTTCACCAGGTTGACATTGCACCAACATTAGCTCTTCTATTTGGTGTTCCAATTCCAAAAAACAACGTTGGTGTTCTGATTGCTGAAATTTTTGGGTATTTGTCTGAAGACCAACAGATGAAGGCACTGGAGTTGAATTCCTGGCAATTACTTAGATTATTGCAAGCACAAATACCTGGTTTATCATGTCGAAATTACCCATGTGATGGCTCAAGGGATGATCAAGAGTCCAGAATAGCTAAGTGCTCTGCGAGCATGGAAGCGATGTTTTGCTGCCTATATACAAATGCTGCACTTCTCCAAAAGTCCTGGATGTCTAAGGAAATCTCCAGGTCTAACAGTAGGGAAGACTACACCAGCACGGTTGTAGGATACTATGAGTTTCTCAGAACTGCAAGTGAGTGGTTGTCACGCAGAGCGACTGATAAACCTGTGAGACTACTTACTGTCGGAATTGCTGCAATGCTCCTATCTTGTCTGATATTATTAAGCCTTCTATTTCATACATGCAAAGAAGTCTACCTTAGAGAGCAGTCCCATTTTGACTTAGAGAGCATCAAGCAGACCTGGTATTTAGACGAGACTTTTGTATTAGGCGTAGTATTGATTCTCATTGTAAGTATGGCATCAAGTTCTATGGTAGAAGAAGAGCAATATATTTGGCATTTTGTGGCTTCCACATTGAATTTGCTATTATTGCGTAAAACAATAAAGTGTCCTACAGGGGCAGGAGCACAAGATCTCCGTAGTTCATCCAAGGGAAAAAACAAAATAAGTTTTTGTCGATTGAGTTCCATCTCTGTGGTTCTTATTTCTGGAAGGATATTGAGAGGCTGGCACCAAGGAGGTGTGAATTGGACTAATCTTCCTGACATTTCAAAGTGGCTTGAGCAGGCTGGGACTGATAATGTAAAATCAATTCAGCTAGTCTCATGCCTTCTAGTAATAAGCTTAAGCTTATGTGTTCTTTTTCTATTTGGATTCAACATGAAATTTGTTCTGGTGATTGGATTCAGCTTTCTTATGTCTGGATTGCTGGTTTTGCAACATTTTGTTAAACATCAAGATGGTATGTTTGCGTCATCGAGTTACAGTACAACAACATTGGTACAAATAATTTATGCAATTATGGGTATTTCCACATTTGGGACTGTTGTAGCCTTACCATGGTTCGTACGTTTCTCAACATCTGAGATGGGCTTGAGTCATGATTTGTACATGTCAACTTCTGTTCCCGATGAAGTTCAAAATAACACGCTGCTGGTGAAATTAAGAGAATCTCTGTATCTAGTTGGGTGGGAATACATCTCTTTCTGGTGTCTTCTGCAACTGCTGCTTCAGCAAACAATTAACTCAATGACTATATTGTTGCTACTTGTGCAAATTTTGGCCAGCATGCTGTATTTTTCTTATAGTGGCCTGCATCAGAAGCAATGGGTTGAGATTGCTGCGTTGTACTTTTTGGGAATGGCCGGCCATTTTGCTCTGGGTAACAGCAATTCACTAGCTACTATTGATGTTGCGGGAGCTTTTATGGGAATCTCAAATCACTCGACAGTACTTTCTGGAATTTTAATGTTCATTATCACCTATGCATCCCCAATGTTATTTATCCTTAGCATGGTGATGTATGTCTCTGTGAGGGACACAAGCTATCTCCCATCTCTCGTGAATGCAGATTCCACACATCTCCTCAAACTGATGCTTGGCTTCCCTTGTCTGGTTCCGCTGGGGTTGAACTCCATTTTGCTAACAGCATACACCATCGTATTGCTGATAATGAGGAACCATCTTTTTGTTTGGAGCGTCTTTTCCCCAAAGTACATTTATGTTTGTACCACAACTATATGCGTCTACATCGGTGTATTTGTAGTAGCTACAACAGTGACATATACTTACTGGGTGCTGGGGTTGAGGAAAAAGATGCAATCTAAGCGTGGCCATCACCATTTAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006497 GO:0006505 GO:0006506 GO:0006629 GO:0006643 GO:0006644 GO:0006650 GO:0006661 GO:0006664 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009247 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016254 GO:0016740 GO:0016772 GO:0016780 GO:0019538 GO:0019637 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046467 GO:0046474 GO:0046486 GO:0046488 GO:0051267 GO:0051377 GO:0071704 GO:0090407 GO:0098827 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00563 map00563

Pfam Domains

Protein Families

Phosphodiest

Protein Analysis

1000

Amino Acids

111.56

Weight (kDa)

7.01

Isoelectric Point (pI)

38.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphodiest	PF01663	235 - 313	5.8e-08	Type I phosphodiesterase / nucleotide pyrophosphatase
PIGO_PIGG	PF19316	541 - 978	1.1e-25	GPI ethanolamine phosphate transferase membrane region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005351)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22530
fragaria_vesca	FvH4_1g19740 FvH4_1g19740 FvH4_1g19740 FvH4_1g19740 FvH4_1g19740 FvH4_1g19740
malus_domestica	MD15G1313700.v1.1
prunus_persica	Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1
pyrus_communis	pycom15g27700
rosa_chinensis	RchiOBHm_Chr2g0111321
rosa_laevigata	RLG00000017886
rosa_multiflora	Rmu_sc0001363.1_g000002 Rmu_sc0005033.1_g000002 Rmu_ssc0000075.1_g000001
rosa_roxburghii	Rroxscaffold_2G00132500
rosa_rugosa	Rorug02G0172800
rosa_samantha	Rh2AG225900 Rh2BG237700 Rh2CG228900 Rh2DG233400
rosa_wichuraiana	Rw2G017440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1878
AccB7I	CCANNNNNTGG	2 cut(s)	763, 1247
AccI	GTMKAC	3 cut(s)	868, 1560, 2895
AccII	CGCG	1 cut(s)	53
AciI	CCGC	3 cut(s)	149, 2564, 2768
AclI	AACGTT	1 cut(s)	1107
AclWI	GGATC	1 cut(s)	699
AcoI	YGGCCR	5 cut(s)	562, 2430, 2511, 2515, 2980
AcsI	RAATTY	9 cut(s)	136, 287, 523, 1128, 1177, 1726, 2033, 2424, 2610
AcuI	CTGAAG	2 cut(s)	1167, 2363
AfiI	CCNNNNNNNGG	8 cut(s)	439, 514, 763, 1247, 2187, 2208, 2662, 2761
AflII	CTTAAG	2 cut(s)	617, 1993
AflIII	ACRYGT	3 cut(s)	223, 685, 2259
AgsI	TTSAA	9 cut(s)	59, 475, 574, 1177, 1726, 1920, 2026, 2288, 2779
AhdI	GACNNNNNGTC	1 cut(s)	1024
AjiI	CACGTC	2 cut(s)	273, 514
AjnI	CCWGG	7 cut(s)	507, 1047, 1181, 1216, 1349, 1370, 1607
AjuI	GAANNNNNNNTTGG	2 cut(s)	324, 356
AleI	CACNNNNGTG	1 cut(s)	151
Alw21I	GWGCWC	4 cut(s)	20, 148, 1289, 1777
Alw26I	GTCTC	4 cut(s)	1471, 1616, 1975, 2680
Alw44I	GTGCAC	1 cut(s)	16
AlwI	GGATC	1 cut(s)	699
AlwNI	CAGNNNCTG	1 cut(s)	403
AoxI	GGCC	8 cut(s)	67, 562, 813, 2430, 2458, 2511, 2515, 2980
ApaLI	GTGCAC	1 cut(s)	16
ApeKI	GCWGC	8 cut(s)	253, 443, 1314, 1331, 1499, 2299, 2374, 2489
ApoI	RAATTY	9 cut(s)	136, 287, 523, 1128, 1177, 1726, 2033, 2424, 2610
ArsI	GACNNNNNNTTYG	2 cut(s)	1610, 1642
AspLEI	GCGC	1 cut(s)	798
AspS9I	GGNCC	2 cut(s)	814, 954
AsuC2I	CCSGG	2 cut(s)	117, 630
AvaII	GGWCC	1 cut(s)	954
BaeGI	GKGCMC	1 cut(s)	20
BalI	TGGCCA	2 cut(s)	2432, 2982
BanI	GGYRCC	1 cut(s)	1878
BauI	CACGAG	2 cut(s)	335, 2708
BbsI	GAAGAC	5 cut(s)	76, 314, 1155, 1395, 2354
Bbv12I	GWGCWC	4 cut(s)	20, 148, 1289, 1777
BbvI	GCAGC	8 cut(s)	265, 430, 1301, 1318, 1486, 2286, 2361, 2476
BciT130I	CCWGG	7 cut(s)	509, 1049, 1183, 1218, 1351, 1372, 1609
BciVI	GTATCC	1 cut(s)	1406
BclI	TGATCA	1 cut(s)	1261
BcnI	CCSGG	2 cut(s)	117, 630
BcoDI	GTCTC	4 cut(s)	1471, 1616, 1975, 2680
BfaI	CTAG	4 cut(s)	1967, 1982, 2330, 2546
BfmI	CTRYAG	1 cut(s)	1761
BfrI	CTTAAG	2 cut(s)	617, 1993
BfuI	GTATCC	1 cut(s)	1406
BglI	GCCNNNNNGGC	1 cut(s)	812
BglII	AGATCT	1 cut(s)	1780
BisI	GCNGC	8 cut(s)	254, 444, 1315, 1332, 1500, 2300, 2375, 2490
BlsI	GCNGC	8 cut(s)	255, 445, 1316, 1333, 1501, 2301, 2376, 2491
BmcAI	AGTACT	1 cut(s)	2601
Bme1390I	CCNGG	9 cut(s)	117, 509, 630, 1049, 1183, 1218, 1351, 1372, 1609
Bme18I	GGWCC	1 cut(s)	954
BmeRI	GACNNNNNGTC	1 cut(s)	1024
BmgBI	CACGTC	2 cut(s)	273, 514
BmgT120I	GGNCC	2 cut(s)	814, 954
BmiI	GGNNCC	6 cut(s)	431, 816, 955, 1880, 2766, 2828
BmrFI	CCNGG	9 cut(s)	117, 509, 630, 1049, 1183, 1218, 1351, 1372, 1609
BmrI	ACTGGG	2 cut(s)	748, 2950
BmsI	GCATC	8 cut(s)	199, 585, 1605, 1676, 2473, 2645, 2733, 2955
BmuI	ACTGGG	2 cut(s)	748, 2950
BpiI	GAAGAC	5 cut(s)	76, 314, 1155, 1395, 2354
BpmI	CTGGAG	4 cut(s)	66, 510, 1190, 1354
Bpu10I	CCTNAGC	3 cut(s)	203, 979, 2657
BpuEI	CTTGAG	3 cut(s)	1237, 1949, 2263
BpuMI	CCSGG	2 cut(s)	117, 630
BsaBI	GATNNNNATC	2 cut(s)	189, 552
BsaJI	CCNNGG	5 cut(s)	5, 922, 1797, 1882, 2207
BsaXI	ACNNNNNCTCC	2 cut(s)	1767, 1797
Bsc4I	CCNNNNNNNGG	8 cut(s)	439, 514, 763, 1247, 2187, 2208, 2662, 2761
Bse118I	RCCGGY	1 cut(s)	2513
Bse1I	ACTGG	4 cut(s)	565, 743, 1173, 2945
Bse3DI	GCAATG	3 cut(s)	1056, 1509, 2480
Bse8I	GATNNNNATC	2 cut(s)	189, 552
BseBI	CCWGG	7 cut(s)	509, 1049, 1183, 1218, 1351, 1372, 1609
BseDI	CCNNGG	5 cut(s)	5, 922, 1797, 1882, 2207
BseGI	GGATG	7 cut(s)	190, 355, 841, 1264, 1359, 1793, 2636
BseJI	GATNNNNATC	2 cut(s)	189, 552
BseLI	CCNNNNNNNGG	8 cut(s)	439, 514, 763, 1247, 2187, 2208, 2662, 2761
BseMI	GCAATG	3 cut(s)	1056, 1509, 2480
BseMII	CTCAG	5 cut(s)	217, 410, 924, 1441, 2223
BseNI	ACTGG	4 cut(s)	565, 743, 1173, 2945
BseRI	GAGGAG	1 cut(s)	2723
BseSI	GKGCMC	1 cut(s)	20
BseXI	GCAGC	8 cut(s)	265, 430, 1301, 1318, 1486, 2286, 2361, 2476
BseYI	CCCAGC	3 cut(s)	1937, 2770, 2947
BsgI	GTGCAG	2 cut(s)	429, 1317
Bsh1236I	CGCG	1 cut(s)	53
BshFI	GGCC	8 cut(s)	69, 564, 815, 2432, 2460, 2513, 2517, 2982
BshNI	GGYRCC	1 cut(s)	1878
BsiHKAI	GWGCWC	4 cut(s)	20, 148, 1289, 1777
BsiSI	CCGG	3 cut(s)	117, 630, 2514
BsiWI	CGTACG	1 cut(s)	2215
BslFI	GGGAC	4 cut(s)	1561, 1954, 2203, 2699
BslI	CCNNNNNNNGG	8 cut(s)	439, 514, 763, 1247, 2187, 2208, 2662, 2761
BsmAI	GTCTC	4 cut(s)	1471, 1616, 1975, 2680
BsmFI	GGGAC	4 cut(s)	1561, 1954, 2203, 2699
BsmI	GAATGC	1 cut(s)	2719
BsnI	GGCC	8 cut(s)	69, 564, 815, 2432, 2460, 2513, 2517, 2982
Bsp1286I	GDGCHC	4 cut(s)	20, 148, 1289, 1777
Bsp1407I	TGTACA	2 cut(s)	682, 2256
Bsp143I	GATC	5 cut(s)	184, 691, 772, 1261, 1780
Bsp19I	CCATGG	3 cut(s)	5, 922, 2207
Bsp68I	TCGCGA	1 cut(s)	53
BspACI	CCGC	3 cut(s)	149, 2564, 2768
BspANI	GGCC	8 cut(s)	69, 564, 815, 2432, 2460, 2513, 2517, 2982
BspCNI	CTCAG	5 cut(s)	216, 409, 925, 1440, 2224
BspFNI	CGCG	1 cut(s)	53
BspHI	TCATGA	1 cut(s)	2248
BspLI	GGNNCC	6 cut(s)	431, 816, 955, 1880, 2766, 2828
BspPI	GGATC	1 cut(s)	699
BspQI	GCTCTTC	2 cut(s)	1080, 1683
BspT107I	GGYRCC	1 cut(s)	1878
BspTI	CTTAAG	2 cut(s)	617, 1993
BsrDI	GCAATG	3 cut(s)	1056, 1509, 2480
BsrFI	RCCGGY	1 cut(s)	2513
BsrGI	TGTACA	2 cut(s)	682, 2256
BsrI	ACTGG	4 cut(s)	565, 743, 1173, 2945
BssAI	RCCGGY	1 cut(s)	2513
BssECI	CCNNGG	5 cut(s)	5, 922, 1797, 1882, 2207
BssMI	GATC	5 cut(s)	184, 691, 772, 1261, 1780
BssNAI	GTATAC	1 cut(s)	869
BssSI	CACGAG	2 cut(s)	335, 2708
BssT1I	CCWWGG	5 cut(s)	5, 922, 1797, 1882, 2207
Bst1107I	GTATAC	1 cut(s)	869
Bst2BI	CACGAG	2 cut(s)	335, 2708
Bst2UI	CCWGG	7 cut(s)	509, 1049, 1183, 1218, 1351, 1372, 1609
Bst6I	CTCTTC	2 cut(s)	1080, 1683
BstAFI	CTTAAG	2 cut(s)	617, 1993
BstAPI	GCANNNNNTGC	2 cut(s)	443, 2374
BstAUI	TGTACA	2 cut(s)	682, 2256
BstC8I	GCNNGC	9 cut(s)	408, 1206, 1294, 1877, 1935, 2434, 2438, 2462, 2515
BstDSI	CCRYGG	3 cut(s)	5, 922, 2207
BstF5I	GGATG	7 cut(s)	190, 355, 841, 1264, 1359, 1793, 2636
BstFNI	CGCG	1 cut(s)	53
BstHHI	GCGC	1 cut(s)	798
BstKTI	GATC	5 cut(s)	187, 694, 775, 1264, 1783
BstMAI	GTCTC	4 cut(s)	1471, 1616, 1975, 2680
BstMBI	GATC	5 cut(s)	184, 691, 772, 1261, 1780
BstNI	CCWGG	7 cut(s)	509, 1049, 1183, 1218, 1351, 1372, 1609
BstNSI	RCATGY	5 cut(s)	227, 689, 1551, 2263, 2440
BstSCI	CCNGG	9 cut(s)	115, 507, 628, 1047, 1181, 1216, 1349, 1370, 1607
BstSFI	CTRYAG	1 cut(s)	1761
BstSLI	GKGCMC	1 cut(s)	20
BstUI	CGCG	1 cut(s)	53
BstV1I	GCAGC	8 cut(s)	265, 430, 1301, 1318, 1486, 2286, 2361, 2476
BstV2I	GAAGAC	5 cut(s)	76, 314, 1155, 1395, 2354
BstX2I	RGATCY	1 cut(s)	1780
BstXI	CCANNNNNNTGG	1 cut(s)	1843
BstYI	RGATCY	1 cut(s)	1780
BstZ17I	GTATAC	1 cut(s)	869
BsuI	GTATCC	1 cut(s)	1406
BsuRI	GGCC	8 cut(s)	69, 564, 815, 2432, 2460, 2513, 2517, 2982
BtgI	CCRYGG	3 cut(s)	5, 922, 2207
BtgZI	GCGATG	1 cut(s)	1319
BtrI	CACGTC	2 cut(s)	273, 514
BtsCI	GGATG	7 cut(s)	190, 355, 841, 1264, 1359, 1793, 2636
BtsI	GCAGTG	2 cut(s)	158, 401
BtsIMutI	CAGTG	6 cut(s)	130, 158, 401, 808, 1166, 2931
BtuMI	TCGCGA	1 cut(s)	53
Cac8I	GCNNGC	9 cut(s)	408, 1206, 1294, 1877, 1935, 2434, 2438, 2462, 2515
CaiI	CAGNNNCTG	1 cut(s)	403
CciI	TCATGA	1 cut(s)	2248
CfoI	GCGC	1 cut(s)	798
Cfr10I	RCCGGY	1 cut(s)	2513
Cfr13I	GGNCC	2 cut(s)	814, 954
CseI	GACGC	4 cut(s)	37, 2109, 2836, 2881
CsiI	ACCWGGT	3 cut(s)	1047, 1216, 1607
DpnI	GATC	5 cut(s)	186, 693, 774, 1263, 1782
DpnII	GATC	5 cut(s)	184, 691, 772, 1261, 1780
DriI	GACNNNNNGTC	1 cut(s)	1024
EaeI	YGGCCR	5 cut(s)	562, 2430, 2511, 2515, 2980
Eam1104I	CTCTTC	2 cut(s)	1080, 1683
Eam1105I	GACNNNNNGTC	1 cut(s)	1024
EarI	CTCTTC	2 cut(s)	1080, 1683
Eco130I	CCWWGG	5 cut(s)	5, 922, 1797, 1882, 2207
Eco47I	GGWCC	1 cut(s)	954
Eco57I	CTGAAG	2 cut(s)	1167, 2363
EcoO109I	RGGNCCY	1 cut(s)	954
EcoRI	GAATTC	1 cut(s)	1177
EcoRII	CCWGG	7 cut(s)	507, 1047, 1181, 1216, 1349, 1370, 1607
EcoT14I	CCWWGG	5 cut(s)	5, 922, 1797, 1882, 2207
EcoT22I	ATGCAT	1 cut(s)	2638
ErhI	CCWWGG	5 cut(s)	5, 922, 1797, 1882, 2207
FalI	AAGNNNNNCTT	2 cut(s)	1548, 1580
FaqI	GGGAC	4 cut(s)	1561, 1954, 2203, 2699
FauI	CCCGC	1 cut(s)	2557
FbaI	TGATCA	1 cut(s)	1261
FblI	GTMKAC	3 cut(s)	868, 1560, 2895
Fnu4HI	GCNGC	8 cut(s)	254, 444, 1315, 1332, 1500, 2300, 2375, 2490
FokI	GGATG	7 cut(s)	177, 362, 848, 1271, 1366, 1780, 2623
FseI	GGCCGGCC	1 cut(s)	2517
Fsp4HI	GCNGC	8 cut(s)	254, 444, 1315, 1332, 1500, 2300, 2375, 2490
FspBI	CTAG	4 cut(s)	1967, 1982, 2330, 2546
GlaI	GCGC	1 cut(s)	797
GluI	GCNGC	8 cut(s)	254, 444, 1315, 1332, 1500, 2300, 2375, 2490
GsaI	CCCAGC	3 cut(s)	1941, 2774, 2951
GsuI	CTGGAG	4 cut(s)	66, 510, 1190, 1354
HaeIII	GGCC	8 cut(s)	69, 564, 815, 2432, 2460, 2513, 2517, 2982
HapII	CCGG	3 cut(s)	117, 630, 2514
HgaI	GACGC	4 cut(s)	37, 2109, 2836, 2881
HhaI	GCGC	1 cut(s)	798
Hin6I	GCGC	1 cut(s)	796
HinP1I	GCGC	1 cut(s)	796
HincII	GTYRAC	2 cut(s)	1054, 2265
HindII	GTYRAC	2 cut(s)	1054, 2265
HindIII	AAGCTT	2 cut(s)	1990, 1996
HpaII	CCGG	3 cut(s)	117, 630, 2514
Hpy166II	GTNNAC	8 cut(s)	18, 869, 1029, 1045, 1054, 1561, 2265, 2896
Hpy8I	GTNNAC	8 cut(s)	18, 869, 1029, 1045, 1054, 1561, 2265, 2896
HpyAV	CCTTC	7 cut(s)	122, 469, 884, 1156, 1544, 1854, 1988
HpyCH4IV	ACGT	5 cut(s)	14, 272, 513, 1107, 2218
HpySE526I	ACGT	5 cut(s)	14, 272, 513, 1107, 2218
HspAI	GCGC	1 cut(s)	796
KroI	GCCGGC	1 cut(s)	2513
KroNI	GCCGGC	1 cut(s)	2515
Ksp22I	TGATCA	1 cut(s)	1261
Kzo9I	GATC	5 cut(s)	184, 691, 772, 1261, 1780
LguI	GCTCTTC	2 cut(s)	1080, 1683
LmnI	GCTCC	5 cut(s)	491, 1512, 1772, 2567, 2844
Lsp1109I	GCAGC	8 cut(s)	265, 430, 1301, 1318, 1486, 2286, 2361, 2476
LweI	GCATC	8 cut(s)	199, 585, 1605, 1676, 2473, 2645, 2733, 2955
MabI	ACCWGGT	3 cut(s)	1047, 1216, 1607
MaeI	CTAG	4 cut(s)	1967, 1982, 2330, 2546
MaeII	ACGT	5 cut(s)	14, 272, 513, 1107, 2218
MaeIII	GTNAC	8 cut(s)	207, 399, 457, 917, 1449, 2129, 2531, 2926
MalI	GATC	5 cut(s)	186, 693, 774, 1263, 1782
MboI	GATC	5 cut(s)	184, 691, 772, 1261, 1780
MfeI	CAATTG	1 cut(s)	423
MflI	RGATCY	1 cut(s)	1780
MhlI	GDGCHC	4 cut(s)	20, 148, 1289, 1777
MlsI	TGGCCA	2 cut(s)	2432, 2982
MluNI	TGGCCA	2 cut(s)	2432, 2982
MlyI	GAGTC	5 cut(s)	57, 663, 968, 1277, 2254
MmeI	TCCRAC	4 cut(s)	476, 563, 760, 1471
Mox20I	TGGCCA	2 cut(s)	2432, 2982
Mph1103I	ATGCAT	1 cut(s)	2638
MroNI	GCCGGC	1 cut(s)	2513
MscI	TGGCCA	2 cut(s)	2432, 2982
MslI	CAYNNNNRTG	6 cut(s)	151, 789, 995, 1841, 2106, 2666
Msp20I	TGGCCA	2 cut(s)	2432, 2982
MspA1I	CMGCKG	1 cut(s)	2770
MspCI	CTTAAG	2 cut(s)	617, 1993
MspI	CCGG	3 cut(s)	117, 630, 2514
MspR9I	CCNGG	9 cut(s)	117, 509, 630, 1049, 1183, 1218, 1351, 1372, 1609
MunI	CAATTG	1 cut(s)	423
Mva1269I	GAATGC	1 cut(s)	2719
MvaI	CCWGG	7 cut(s)	509, 1049, 1183, 1218, 1351, 1372, 1609
MvnI	CGCG	1 cut(s)	53
NaeI	GCCGGC	1 cut(s)	2515
NciI	CCSGG	2 cut(s)	117, 630
NcoI	CCATGG	3 cut(s)	5, 922, 2207
NdeII	GATC	5 cut(s)	184, 691, 772, 1261, 1780
NgoMIV	GCCGGC	1 cut(s)	2513
NlaIV	GGNNCC	6 cut(s)	431, 816, 955, 1880, 2766, 2828
NmeAIII	GCCGAG	1 cut(s)	540
NmuCI	GTSAC	5 cut(s)	207, 399, 917, 1449, 2926
NruI	TCGCGA	1 cut(s)	53
NsiI	ATGCAT	1 cut(s)	2638
NspI	RCATGY	5 cut(s)	227, 689, 1551, 2263, 2440
OliI	CACNNNNGTG	1 cut(s)	151
PaeI	GCATGC	1 cut(s)	2440
PagI	TCATGA	1 cut(s)	2248
PciI	ACATGT	3 cut(s)	223, 685, 2259
PciSI	GCTCTTC	2 cut(s)	1080, 1683
PcsI	WCGNNNNNNNCGW	1 cut(s)	288
PctI	GAATGC	1 cut(s)	2719
PdiI	GCCGGC	1 cut(s)	2515
Pfl23II	CGTACG	1 cut(s)	2215
PflMI	CCANNNNNTGG	2 cut(s)	763, 1247
PfoI	TCCNGGA	4 cut(s)	115, 507, 628, 1349
PkrI	GCNGC	8 cut(s)	255, 445, 1316, 1333, 1501, 2301, 2376, 2491
PleI	GAGTC	5 cut(s)	56, 662, 968, 1276, 2253
PpsI	GAGTC	5 cut(s)	56, 662, 968, 1276, 2253
PpuMI	RGGWCCY	1 cut(s)	954
PscI	ACATGT	3 cut(s)	223, 685, 2259
Psp1406I	AACGTT	1 cut(s)	1107
Psp5II	RGGWCCY	1 cut(s)	954
Psp6I	CCWGG	7 cut(s)	507, 1047, 1181, 1216, 1349, 1370, 1607
PspFI	CCCAGC	3 cut(s)	1937, 2770, 2947
PspGI	CCWGG	7 cut(s)	507, 1047, 1181, 1216, 1349, 1370, 1607
PspLI	CGTACG	1 cut(s)	2215
PspN4I	GGNNCC	6 cut(s)	431, 816, 955, 1880, 2766, 2828
PspPI	GGNCC	2 cut(s)	814, 954
PspPPI	RGGWCCY	1 cut(s)	954
PstNI	CAGNNNCTG	1 cut(s)	403
PsuI	RGATCY	1 cut(s)	1780
RigI	GGCCGGCC	1 cut(s)	2517
RruI	TCGCGA	1 cut(s)	53
RseI	CAYNNNNRTG	6 cut(s)	151, 789, 995, 1841, 2106, 2666
SapI	GCTCTTC	2 cut(s)	1080, 1683
SatI	GCNGC	8 cut(s)	254, 444, 1315, 1332, 1500, 2300, 2375, 2490
Sau3AI	GATC	5 cut(s)	184, 691, 772, 1261, 1780
Sau96I	GGNCC	2 cut(s)	814, 954
ScaI	AGTACT	1 cut(s)	2601
SchI	GAGTC	5 cut(s)	57, 663, 968, 1277, 2254
ScrFI	CCNGG	9 cut(s)	117, 509, 630, 1049, 1183, 1218, 1351, 1372, 1609
SduI	GDGCHC	4 cut(s)	20, 148, 1289, 1777
SexAI	ACCWGGT	3 cut(s)	1047, 1216, 1607
SfaNI	GCATC	8 cut(s)	199, 585, 1605, 1676, 2473, 2645, 2733, 2955
SfcI	CTRYAG	1 cut(s)	1761
SinI	GGWCC	1 cut(s)	954
SmiMI	CAYNNNNRTG	6 cut(s)	151, 789, 995, 1841, 2106, 2666
SmlI	CTYRAG	5 cut(s)	617, 1252, 1928, 1993, 2242
SmoI	CTYRAG	5 cut(s)	617, 1252, 1928, 1993, 2242
SphI	GCATGC	1 cut(s)	2440
SsiI	CCGC	3 cut(s)	149, 2564, 2768
SspMI	CTAG	4 cut(s)	1967, 1982, 2330, 2546
StyD4I	CCNGG	9 cut(s)	115, 507, 628, 1047, 1181, 1216, 1349, 1370, 1607
StyI	CCWWGG	5 cut(s)	5, 922, 1797, 1882, 2207
TaiI	ACGT	5 cut(s)	17, 275, 516, 1110, 2221
TaqI	TCGA	8 cut(s)	142, 282, 706, 826, 1231, 1825, 2126, 2594
TatI	WGTACW	6 cut(s)	682, 2135, 2256, 2496, 2599, 2862
TscAI	CASTG	6 cut(s)	130, 158, 408, 808, 1173, 2931
TseFI	GTSAC	5 cut(s)	207, 399, 917, 1449, 2926
TseI	GCWGC	8 cut(s)	253, 443, 1314, 1331, 1499, 2299, 2374, 2489
Tsp45I	GTSAC	5 cut(s)	207, 399, 917, 1449, 2926
TspGWI	ACGGA	2 cut(s)	963, 1775
TspRI	CASTG	6 cut(s)	130, 158, 408, 808, 1173, 2931
Van91I	CCANNNNNTGG	2 cut(s)	763, 1247
Vha464I	CTTAAG	2 cut(s)	617, 1993
VneI	GTGCAC	1 cut(s)	16
VpaK11BI	GGWCC	1 cut(s)	954
XapI	RAATTY	9 cut(s)	136, 287, 523, 1128, 1177, 1726, 2033, 2424, 2610
XceI	RCATGY	5 cut(s)	227, 689, 1551, 2263, 2440
XmiI	GTMKAC	3 cut(s)	868, 1560, 2895
XspI	CTAG	4 cut(s)	1967, 1982, 2330, 2546
ZrmI	AGTACT	1 cut(s)	2601
Zsp2I	ATGCAT	1 cut(s)	2638

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.