pycom15g28170

Pentatricopeptide repeat-containing protein At4g02750-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016737
Physical Location & Seq
Forward (+)
23905565 .. 23907503
1939 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1752 bp
ATGCGATTTGTTCGATTGCCTGTCCGTGTCCGTGCCCATGTCAACTTCATTGCAGATACTTGTTGCATCCTTCACTGCTTGATTTCTCTACGGTTGAAGCTTTTCTCAACTTATGATGTATATACTTGCAATGTCAAGATTGGTGTGTTATCAAGAGCGGGAAAGATTGAAGCCGCCCGCCAATTGTTCGATGAAATGCCCACAAAAGACGTAGTTACATGGAACGCCATTATTACTGGGTACTGGAAAAATGGGTATTTTGGAGAGTCCAAGAGATTGTTTCGGCTGATGCCTGTTCGGAATGTGGTGTCCTGGAATTCCATGTTAGCCGGGAGTGTCGAGAATGAGATGGTCGACGAGGCTTTCATGTACTTCCGGGAGATGCCAGAAAGAAATATTGCTTCTTGGAATGCTATGATTTCGGGGTTTGTGAAATGTGATAGGCTGGAGGAAGCTAGTAGGATATTTCAAGACATGCCGAAGAGGAATGTTATTTCTTATACTGCAATGATTGATGGGTACGCGATGAAAGGAGAGATAGAGCGGGCAAGGGCACTGTTTGACTGCATGCCTCGCAAGAATGCCGTTTCTTGGACAGTGCTGATCAGTGGGTATGTGGAGAACGGCAAATTTGATGAAGCACGGGAATTGTATGAGCAGATGCCGGAGAAAAATGCGGTTTCAATGACAGCTATGCTCACGGGGTATTCTAAGGAGGGAAAGATGGAAGATGCGAGAGCATTATTTGATCAGATTCAGCGCAAGGATCATGTCTCTTGGAATGCAATGATAACAGGCTATGCACAAAATGGCGGCGGAGAGGAAGCACTGAAGTTACACTCACAAAAGCTTAAGATTGGTCTGCATCCAGATAAATGGACCCTTGTTTCGGTTCTGACTGCCTGTTCTAGTCTTACATCTCTTAAAGACGGAAAACAAGCCCATCTGCTCATTATTAAACATGGATTTGAATCAAACCTCTCCTTATATAACGCTTTGATTACCATGTACAATAAATGTGGTGCAATCCTTGATGCCGAGTTAGTGTTCAAACAAATTGAGTGTCCAGACCTCGTCTCATGGAACACCATTATTGCTGCATTTGCACAGCATGGTCTCTATGAGAAAGCTCTTGATTTCTTCAACCAGACTGTGGTGCACGGCTTCAAACCAGATGGTATAACATTTCTTAGCTTGCTATCTGCCTGTGCTCATGCAGGCAAGGTAAACGAGAGCTTGAAGTTGTTTGATTTGATGGTCAGCAGCTACAACATTGCCCCTAGATCGGAGCATTATGCTTGCTTGGTTGACATTTTGTGTCGAGCAGGCCAGTTAGAGAAGGCTTGCAAGATGATCCAAGAGATGCCATTTCAAGCAGACTCTGGAATATGGGGCTCTCTTCTTGCAGCTTGCAGCATTTACTTAAACGTGGAAATAGGGGAGCTGGCTGCAAAGAATATATTACATTTGAATCCTCGTAGTTCTGGGCCTTACGTTGCGTTGTCTAACATATACGCAGCTGCCGGAAAATGGAGAGATGTTGCAAGAATGAGAAGTCTGATGAAAGAGCATGGAGTGAAGAAGCAACAGGCACAGAGCTGGACAGAGATTGCGAATAAGGTGCACATTTTCTTGGGAGGGGATGTATCTCATCCAGATATTGATAAGATTCATTTGATGCTCAAAACGATCAGTCTCCATATACCTGCGGATGATTTTGTAGACATTACGTTTCATGGAGCTACTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

584

Amino Acids

65.64

Weight (kDa)

8.26

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 100 - 133 3.4e-06 PPR repeat family
PPR PF01535 103 - 133 9.7e-06 PPR repeat
PPR_2 PF13041 132 - 164 1.9e-07 PPR repeat family
PPR PF01535 135 - 164 2.2e-07 PPR repeat
PPR PF01535 165 - 194 2.2e-06 PPR repeat
PPR_2 PF13041 193 - 225 2e-07 PPR repeat family
PPR PF01535 196 - 225 2e-08 PPR repeat
PPR_2 PF13041 256 - 303 2.6e-07 PPR repeat family
PPR_2 PF13041 356 - 405 3.6e-11 PPR repeat family
PPR PF01535 359 - 388 9.6e-06 PPR repeat
E_motif PF20431 474 - 535 3.1e-18 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016737)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20280
malus_domestica MD01G1035200.v1.1 MD15G1320700.v1.1
prunus_persica Prupe.6G192600_v2.0.a1
pyrus_communis pycom01g06230 pycom15g28170
rosa_chinensis RchiOBHm_Chr2g0112421
rosa_laevigata RLG00000017979
rosa_multiflora Rmu_sc0031542.1_g000001
rosa_rugosa Rorug02G0180400
rosa_samantha Rh2BG247100 Rh2CG238200 Rh2DG241400
rosa_wichuraiana Rw2G018100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1714
AccB7I CCANNNNNTGG 1 cut(s) 1153
AccBSI CCGCTC 2 cut(s) 158, 544
AccI GTMKAC 2 cut(s) 354, 1722
AccII CGCG 1 cut(s) 524
AciI CCGC 8 cut(s) 158, 174, 178, 544, 677, 813, 816, 1709
AclWI GGATC 2 cut(s) 774, 1348
AcsI RAATTY 2 cut(s) 316, 629
AcuI CTGAAG 1 cut(s) 851
AfaI GTAC 4 cut(s) 242, 371, 521, 1010
AfiI CCNNNNNNNGG 5 cut(s) 591, 889, 1153, 1285, 1530
AflII CTTAAG 1 cut(s) 851
AjnI CCWGG 1 cut(s) 311
Alw21I GWGCWC 3 cut(s) 1161, 1213, 1626
Alw26I GTCTC 4 cut(s) 778, 1081, 1121, 1700
Alw44I GTGCAC 2 cut(s) 1157, 1622
AlwI GGATC 2 cut(s) 774, 1348
AlwNI CAGNNNCTG 1 cut(s) 1382
AoxI GGCC 2 cut(s) 1327, 1487
ApaLI GTGCAC 2 cut(s) 1157, 1622
ApeKI GCWGC 7 cut(s) 1097, 1263, 1406, 1413, 1448, 1517, 1520
ApoI RAATTY 2 cut(s) 316, 629
ArsI GACNNNNNNTTYG 4 cut(s) 871, 903, 1048, 1080
Asp700I GAANNNNTTC 1 cut(s) 101
AspLEI GCGC 1 cut(s) 762
AspS9I GGNCC 2 cut(s) 879, 1487
AsuC2I CCSGG 2 cut(s) 331, 377
AvaII GGWCC 1 cut(s) 879
BaeGI GKGCMC 4 cut(s) 37, 556, 1161, 1626
BanII GRGCYC 1 cut(s) 1397
Bbv12I GWGCWC 3 cut(s) 1161, 1213, 1626
BbvI GCAGC 7 cut(s) 1084, 1275, 1418, 1425, 1435, 1507, 1529
BccI CCATC 6 cut(s) 343, 509, 718, 951, 1169, 1249
BceAI ACGGC 3 cut(s) 569, 640, 1177
BcgI CGANNNNNNTGC 2 cut(s) 1603, 1637
BciT130I CCWGG 1 cut(s) 313
BclI TGATCA 2 cut(s) 603, 748
BcnI CCSGG 2 cut(s) 331, 377
BcoDI GTCTC 4 cut(s) 778, 1081, 1121, 1700
BfaI CTAG 4 cut(s) 456, 909, 1281, 1746
BfrI CTTAAG 1 cut(s) 851
BfuAI ACCTGC 1 cut(s) 1714
BisI GCNGC 9 cut(s) 174, 814, 1098, 1264, 1407, 1414, 1449, 1518, 1521
BlsI GCNGC 9 cut(s) 175, 815, 1099, 1265, 1408, 1415, 1450, 1519, 1522
Bme1390I CCNGG 3 cut(s) 313, 331, 377
Bme18I GGWCC 1 cut(s) 879
BmgT120I GGNCC 2 cut(s) 879, 1487
BmiI GGNNCC 1 cut(s) 881
BmrFI CCNGG 3 cut(s) 313, 331, 377
BmrI ACTGGG 1 cut(s) 246
BmsI GCATC 9 cut(s) 75, 279, 372, 651, 721, 874, 1024, 1353, 1668
BmuI ACTGGG 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 467
BpuMI CCSGG 2 cut(s) 331, 377
BsaBI GATNNNNATC 1 cut(s) 970
BsaI GGTCTC 1 cut(s) 1121
BsaXI ACNNNNNCTCC 2 cut(s) 1629, 1659
Bsc4I CCNNNNNNNGG 5 cut(s) 591, 889, 1153, 1285, 1530
Bse1I ACTGG 3 cut(s) 241, 248, 1330
Bse3DI GCAATG 5 cut(s) 48, 136, 513, 792, 1272
Bse8I GATNNNNATC 1 cut(s) 970
BseBI CCWGG 1 cut(s) 313
BseGI GGATG 5 cut(s) 66, 865, 1648, 1651, 1717
BseJI GATNNNNATC 1 cut(s) 970
BseLI CCNNNNNNNGG 5 cut(s) 591, 889, 1153, 1285, 1530
BseMI GCAATG 5 cut(s) 48, 136, 513, 792, 1272
BseNI ACTGG 3 cut(s) 241, 248, 1330
BseSI GKGCMC 4 cut(s) 37, 556, 1161, 1626
BseXI GCAGC 7 cut(s) 1084, 1275, 1418, 1425, 1435, 1507, 1529
Bsh1236I CGCG 1 cut(s) 524
BshFI GGCC 2 cut(s) 1329, 1489
BsiHKAI GWGCWC 3 cut(s) 1161, 1213, 1626
BsiSI CCGG 4 cut(s) 330, 376, 665, 1524
BslI CCNNNNNNNGG 5 cut(s) 591, 889, 1153, 1285, 1530
BsmAI GTCTC 4 cut(s) 778, 1081, 1121, 1700
BsmBI CGTCTC 1 cut(s) 1081
BsmI GAATGC 3 cut(s) 415, 586, 787
BsnI GGCC 2 cut(s) 1329, 1489
Bso31I GGTCTC 1 cut(s) 1121
Bsp1286I GDGCHC 6 cut(s) 37, 556, 1161, 1213, 1397, 1626
Bsp1407I TGTACA 1 cut(s) 1008
Bsp143I GATC 6 cut(s) 603, 748, 766, 1283, 1353, 1689
BspACI CCGC 8 cut(s) 158, 174, 178, 544, 677, 813, 816, 1709
BspANI GGCC 2 cut(s) 1329, 1489
BspFNI CGCG 1 cut(s) 524
BspLI GGNNCC 1 cut(s) 881
BspMI ACCTGC 1 cut(s) 1714
BspPI GGATC 2 cut(s) 774, 1348
BspTI CTTAAG 1 cut(s) 851
BspTNI GGTCTC 1 cut(s) 1121
BsrBI CCGCTC 2 cut(s) 158, 544
BsrDI GCAATG 5 cut(s) 48, 136, 513, 792, 1272
BsrGI TGTACA 1 cut(s) 1008
BsrI ACTGG 3 cut(s) 241, 248, 1330
BssMI GATC 6 cut(s) 603, 748, 766, 1283, 1353, 1689
Bst2UI CCWGG 1 cut(s) 313
Bst4CI ACNGT 4 cut(s) 93, 558, 598, 1153
Bst6I CTCTTC 2 cut(s) 476, 1404
BstAFI CTTAAG 1 cut(s) 851
BstAUI TGTACA 1 cut(s) 1008
BstDEI CTNAG 2 cut(s) 711, 1190
BstF5I GGATG 5 cut(s) 66, 865, 1648, 1651, 1717
BstFNI CGCG 1 cut(s) 524
BstHHI GCGC 1 cut(s) 762
BstKTI GATC 6 cut(s) 606, 751, 769, 1286, 1356, 1692
BstMAI GTCTC 4 cut(s) 778, 1081, 1121, 1700
BstMBI GATC 6 cut(s) 603, 748, 766, 1283, 1353, 1689
BstMWI GCNNNNNNNGC 2 cut(s) 573, 1103
BstNI CCWGG 1 cut(s) 313
BstNSI RCATGY 2 cut(s) 478, 571
BstSCI CCNGG 3 cut(s) 311, 329, 375
BstSLI GKGCMC 4 cut(s) 37, 556, 1161, 1626
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 7 cut(s) 1084, 1275, 1418, 1425, 1435, 1507, 1529
BstXI CCANNNNNNTGG 1 cut(s) 876
BsuRI GGCC 2 cut(s) 1329, 1489
BtgZI GCGATG 1 cut(s) 539
BtsCI GGATG 5 cut(s) 66, 865, 1648, 1651, 1717
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 5 cut(s) 73, 554, 603, 613, 827
BveI ACCTGC 1 cut(s) 1714
CaiI CAGNNNCTG 1 cut(s) 1382
CfoI GCGC 1 cut(s) 762
Cfr13I GGNCC 2 cut(s) 879, 1487
Csp6I GTAC 4 cut(s) 241, 370, 520, 1009
CspCI CAANNNNNGTGG 2 cut(s) 1000, 1035
CviQI GTAC 4 cut(s) 241, 370, 520, 1009
DdeI CTNAG 2 cut(s) 711, 1190
DpnI GATC 6 cut(s) 605, 750, 768, 1285, 1355, 1691
DpnII GATC 6 cut(s) 603, 748, 766, 1283, 1353, 1689
Eam1104I CTCTTC 2 cut(s) 476, 1404
EarI CTCTTC 2 cut(s) 476, 1404
EciI GGCGGA 1 cut(s) 831
Eco24I GRGCYC 1 cut(s) 1397
Eco31I GGTCTC 1 cut(s) 1121
Eco47I GGWCC 1 cut(s) 879
Eco57I CTGAAG 1 cut(s) 851
EcoRI GAATTC 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 311
EcoT38I GRGCYC 1 cut(s) 1397
Esp3I CGTCTC 1 cut(s) 1081
FauI CCCGC 3 cut(s) 151, 185, 537
FbaI TGATCA 2 cut(s) 603, 748
FblI GTMKAC 2 cut(s) 354, 1722
Fnu4HI GCNGC 9 cut(s) 174, 814, 1098, 1264, 1407, 1414, 1449, 1518, 1521
FokI GGATG 5 cut(s) 53, 852, 1638, 1655, 1724
FriOI GRGCYC 1 cut(s) 1397
Fsp4HI GCNGC 9 cut(s) 174, 814, 1098, 1264, 1407, 1414, 1449, 1518, 1521
FspBI CTAG 4 cut(s) 456, 909, 1281, 1746
GlaI GCGC 1 cut(s) 761
GluI GCNGC 9 cut(s) 174, 814, 1098, 1264, 1407, 1414, 1449, 1518, 1521
GsuI CTGGAG 1 cut(s) 467
HaeIII GGCC 2 cut(s) 1329, 1489
HapII CCGG 4 cut(s) 330, 376, 665, 1524
HhaI GCGC 1 cut(s) 762
Hin6I GCGC 1 cut(s) 760
HinP1I GCGC 1 cut(s) 760
HincII GTYRAC 3 cut(s) 43, 355, 1309
HindII GTYRAC 3 cut(s) 43, 355, 1309
HindIII AAGCTT 2 cut(s) 98, 848
HinfI GANTC 6 cut(s) 266, 754, 971, 1379, 1471, 1669
HpaII CCGG 4 cut(s) 330, 376, 665, 1524
Hpy166II GTNNAC 7 cut(s) 43, 355, 1159, 1228, 1309, 1624, 1723
Hpy188I TCNGA 5 cut(s) 300, 753, 897, 1288, 1560
Hpy188III TCNNGA 9 cut(s) 136, 153, 340, 470, 869, 1067, 1133, 1383, 1655
Hpy8I GTNNAC 7 cut(s) 43, 355, 1159, 1228, 1309, 1624, 1723
Hpy99I CGWCG 1 cut(s) 359
HpyAV CCTTC 2 cut(s) 80, 1333
HpyCH4III ACNGT 4 cut(s) 93, 558, 598, 1153
HpyCH4IV ACGT 4 cut(s) 210, 1428, 1494, 1730
HpyF10VI GCNNNNNNNGC 2 cut(s) 573, 1103
HpyF3I CTNAG 2 cut(s) 711, 1190
HpySE526I ACGT 4 cut(s) 210, 1428, 1494, 1730
HspAI GCGC 1 cut(s) 760
Ksp22I TGATCA 2 cut(s) 603, 748
Kzo9I GATC 6 cut(s) 603, 748, 766, 1283, 1353, 1689
LmnI GCTCC 3 cut(s) 1288, 1441, 1739
Lsp1109I GCAGC 7 cut(s) 1084, 1275, 1418, 1425, 1435, 1507, 1529
LweI GCATC 9 cut(s) 75, 279, 372, 651, 721, 874, 1024, 1353, 1668
MaeI CTAG 4 cut(s) 456, 909, 1281, 1746
MaeII ACGT 4 cut(s) 210, 1428, 1494, 1730
MaeIII GTNAC 2 cut(s) 214, 834
MalI GATC 6 cut(s) 605, 750, 768, 1285, 1355, 1691
MbiI CCGCTC 2 cut(s) 158, 544
MboI GATC 6 cut(s) 603, 748, 766, 1283, 1353, 1689
MboII GAAGA 5 cut(s) 493, 740, 1132, 1391, 1591
MfeI CAATTG 1 cut(s) 182
MhlI GDGCHC 6 cut(s) 37, 556, 1161, 1213, 1397, 1626
MluCI AATT 5 cut(s) 182, 316, 629, 647, 1056
MlyI GAGTC 2 cut(s) 275, 1373
MroXI GAANNNNTTC 1 cut(s) 101
MseI TTAA 4 cut(s) 852, 924, 957, 1424
MspA1I CMGCKG 1 cut(s) 1520
MspCI CTTAAG 1 cut(s) 851
MspI CCGG 4 cut(s) 330, 376, 665, 1524
MspR9I CCNGG 3 cut(s) 313, 331, 377
MunI CAATTG 1 cut(s) 182
Mva1269I GAATGC 3 cut(s) 415, 586, 787
MvaI CCWGG 1 cut(s) 313
MvnI CGCG 1 cut(s) 524
MwoI GCNNNNNNNGC 2 cut(s) 573, 1103
NciI CCSGG 2 cut(s) 331, 377
NdeII GATC 6 cut(s) 603, 748, 766, 1283, 1353, 1689
NlaIV GGNNCC 1 cut(s) 881
NmeAIII GCCGAG 1 cut(s) 1063
NspI RCATGY 2 cut(s) 478, 571
PaeI GCATGC 1 cut(s) 571
PctI GAATGC 3 cut(s) 415, 586, 787
PdmI GAANNNNTTC 1 cut(s) 101
PfeI GAWTC 4 cut(s) 754, 971, 1471, 1669
PflFI GACNNNGTC 1 cut(s) 1073
PflMI CCANNNNNTGG 1 cut(s) 1153
PfoI TCCNGGA 2 cut(s) 311, 375
PkrI GCNGC 9 cut(s) 175, 815, 1099, 1265, 1408, 1415, 1450, 1519, 1522
PleI GAGTC 2 cut(s) 274, 1373
PpsI GAGTC 2 cut(s) 274, 1373
Psp6I CCWGG 1 cut(s) 311
PspGI CCWGG 1 cut(s) 311
PspN4I GGNNCC 1 cut(s) 881
PspPI GGNCC 2 cut(s) 879, 1487
PstNI CAGNNNCTG 1 cut(s) 1382
PsyI GACNNNGTC 1 cut(s) 1073
PvuII CAGCTG 1 cut(s) 1520
RsaI GTAC 4 cut(s) 242, 371, 521, 1010
RsaNI GTAC 4 cut(s) 241, 370, 520, 1009
SalI GTCGAC 1 cut(s) 353
SaqAI TTAA 4 cut(s) 852, 924, 957, 1424
SatI GCNGC 9 cut(s) 174, 814, 1098, 1264, 1407, 1414, 1449, 1518, 1521
Sau3AI GATC 6 cut(s) 603, 748, 766, 1283, 1353, 1689
Sau96I GGNCC 2 cut(s) 879, 1487
SchI GAGTC 2 cut(s) 275, 1373
ScrFI CCNGG 3 cut(s) 313, 331, 377
SduI GDGCHC 6 cut(s) 37, 556, 1161, 1213, 1397, 1626
SfaNI GCATC 9 cut(s) 75, 279, 372, 651, 721, 874, 1024, 1353, 1668
SinI GGWCC 1 cut(s) 879
SmlI CTYRAG 1 cut(s) 851
SmoI CTYRAG 1 cut(s) 851
SphI GCATGC 1 cut(s) 571
Sse9I AATT 5 cut(s) 182, 316, 629, 647, 1056
SsiI CCGC 8 cut(s) 158, 174, 178, 544, 677, 813, 816, 1709
SspI AATATT 1 cut(s) 397
SspMI CTAG 4 cut(s) 456, 909, 1281, 1746
StyD4I CCNGG 3 cut(s) 311, 329, 375
TaaI ACNGT 4 cut(s) 93, 558, 598, 1153
TaiI ACGT 4 cut(s) 213, 1431, 1497, 1733
TaqI TCGA 5 cut(s) 13, 189, 339, 354, 1321
TasI AATT 5 cut(s) 182, 316, 629, 647, 1056
TatI WGTACW 2 cut(s) 369, 1008
TauI GCSGC 2 cut(s) 176, 816
TfiI GAWTC 4 cut(s) 754, 971, 1471, 1669
Tru1I TTAA 4 cut(s) 852, 924, 957, 1424
Tru9I TTAA 4 cut(s) 852, 924, 957, 1424
TscAI CASTG 5 cut(s) 80, 561, 603, 613, 834
TseI GCWGC 7 cut(s) 1097, 1263, 1406, 1413, 1448, 1517, 1520
TspDTI ATGAA 8 cut(s) 37, 207, 355, 542, 651, 1577, 1661, 1724
TspGWI ACGGA 3 cut(s) 14, 20, 945
TspRI CASTG 5 cut(s) 80, 561, 603, 613, 834
Tth111I GACNNNGTC 1 cut(s) 1073
Van91I CCANNNNNTGG 1 cut(s) 1153
Vha464I CTTAAG 1 cut(s) 851
VneI GTGCAC 2 cut(s) 1157, 1622
VpaK11BI GGWCC 1 cut(s) 879
XapI RAATTY 2 cut(s) 316, 629
XceI RCATGY 2 cut(s) 478, 571
XmiI GTMKAC 2 cut(s) 354, 1722
XmnI GAANNNNTTC 1 cut(s) 101
XspI CTAG 4 cut(s) 456, 909, 1281, 1746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.