pycom15g29600

DNA-directed RNA polymerases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015704
Physical Location & Seq
Reverse (-)
26518705 .. 26520552
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 474 bp
ATGATATATTTAGCATTGAGAAAGTGGACCCAGATGGCAAAAAATTTGACAAAGTACTCGGGTCTCGTTGTTGTTAAATCACAGAGTGAGAAGCATGATGTGTTGCTGCTCCTGGATGTGAATACGGAAATATATCCTATGCATGTAAAGGAGAAGTTCTTGATGGTGCTGTCTCCCACACTCGAGTGGAGCGGAGCACCTGTTACTGGAAAACGTGGACAGGTTGAGCAGAAATCTCTTGCTGACAAATTTGAATATATTATGCATGGATTGCTGTACAAAATGTCAACAGCAAGTTCAGAATCTTCAAAAGGAAACCCCAAGGCTTCAGATGAAGGTTCAAAGACTGAGGTCAAAGTGGAGGTATACGCTTCATTTGGTGGGCTTCAGATGATGCTGAAAGGAGATCCTTCGAGTTGTACTAAGTTTAAGATTGATCAGAACTTATTTTTGCTTATCAGGAAATTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05016 ko05169 map00230 map00240 map01100 map03020 map04623 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb8

Protein Analysis

158

Amino Acids

17.69

Weight (kDa)

9.32

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb8 PF03870 11 - 155 2.2e-33 RNA polymerase Rpb8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015704)

Species Orthologous Gene IDs
malus_domestica MD01G1023800.v1.1 MD15G1329500.v1.1
prunus_persica Prupe.6G178800_v2.0.a1
pyrus_communis pycom01g05140 pycom15g29600
rosa_chinensis RchiOBHm_Chr2g0115551
rosa_laevigata RLG00000018193
rosa_multiflora Rmu_ssc0000197.1_g000090
rosa_roxburghii Rroxscaffold_2G00128110
rosa_rugosa Rorug02G0197900 Rorug02G0198000
rosa_samantha Rh2AG255200 Rh2BG265900 Rh2CG260400 Rh2DG263400
rosa_wichuraiana Rw2G019980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 192
AccI GTMKAC 1 cut(s) 366
AciI CCGC 1 cut(s) 192
AclWI GGATC 1 cut(s) 401
AcsI RAATTY 2 cut(s) 43, 248
AcuI CTGAAG 2 cut(s) 312, 371
AdeI CACNNNGTG 1 cut(s) 86
AfaI GTAC 3 cut(s) 56, 278, 421
AfiI CCNNNNNNNGG 1 cut(s) 206
AgsI TTSAA 3 cut(s) 254, 309, 342
AjnI CCWGG 1 cut(s) 111
AleI CACNNNNGTG 1 cut(s) 184
Alw21I GWGCWC 1 cut(s) 199
Alw26I GTCTC 2 cut(s) 68, 177
AlwI GGATC 1 cut(s) 401
Ama87I CYCGRG 2 cut(s) 58, 182
ApeKI GCWGC 1 cut(s) 106
ApoI RAATTY 2 cut(s) 43, 248
AspS9I GGNCC 1 cut(s) 27
AvaI CYCGRG 2 cut(s) 58, 182
AvaII GGWCC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 199
BbvI GCAGC 1 cut(s) 93
BccI CCATC 2 cut(s) 28, 157
BciT130I CCWGG 1 cut(s) 113
BclI TGATCA 1 cut(s) 436
BcoDI GTCTC 2 cut(s) 68, 177
BisI GCNGC 1 cut(s) 107
BlsI GCNGC 1 cut(s) 108
BmcAI AGTACT 1 cut(s) 56
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 1 cut(s) 27
BmeT110I CYCGRG 2 cut(s) 58, 182
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 1 cut(s) 29
BmrFI CCNGG 1 cut(s) 113
BmsI GCATC 1 cut(s) 384
BoxI GACNNNNGTC 1 cut(s) 350
BsaI GGTCTC 1 cut(s) 68
BsaJI CCNNGG 1 cut(s) 321
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 211
BseBI CCWGG 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 321
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 1 cut(s) 211
BseXI GCAGC 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 199
BsiHKCI CYCGRG 2 cut(s) 58, 182
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 2 cut(s) 68, 177
Bso31I GGTCTC 1 cut(s) 68
BsoBI CYCGRG 2 cut(s) 58, 182
Bsp1286I GDGCHC 1 cut(s) 199
Bsp1407I TGTACA 1 cut(s) 276
Bsp143I GATC 2 cut(s) 406, 436
BspACI CCGC 1 cut(s) 192
BspCNI CTCAG 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 29
BspPI GGATC 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 68
BsrBI CCGCTC 1 cut(s) 192
BsrGI TGTACA 1 cut(s) 276
BsrI ACTGG 1 cut(s) 211
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 2 cut(s) 406, 436
BssNAI GTATAC 1 cut(s) 367
BssT1I CCWWGG 1 cut(s) 321
Bst1107I GTATAC 1 cut(s) 367
Bst2UI CCWGG 1 cut(s) 113
BstAPI GCANNNNNTGC 1 cut(s) 271
BstAUI TGTACA 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 348, 423
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 2 cut(s) 409, 439
BstMAI GTCTC 2 cut(s) 68, 177
BstMBI GATC 2 cut(s) 406, 436
BstMWI GCNNNNNNNGC 1 cut(s) 271
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 1 cut(s) 146
BstPAI GACNNNNGTC 1 cut(s) 350
BstSCI CCNGG 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 93
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BstZ17I GTATAC 1 cut(s) 367
BtsCI GGATG 1 cut(s) 121
Cfr13I GGNCC 1 cut(s) 27
Csp6I GTAC 3 cut(s) 55, 277, 420
CviAII CATG 3 cut(s) 95, 143, 266
CviJI RGCY 2 cut(s) 326, 385
CviKI_1 RGCY 2 cut(s) 326, 385
CviQI GTAC 3 cut(s) 55, 277, 420
DdeI CTNAG 2 cut(s) 348, 423
DpnI GATC 2 cut(s) 408, 438
DpnII GATC 2 cut(s) 406, 436
DraIII CACNNNGTG 1 cut(s) 86
Eco130I CCWWGG 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 68
Eco47I GGWCC 1 cut(s) 27
Eco57I CTGAAG 2 cut(s) 312, 371
Eco88I CYCGRG 2 cut(s) 58, 182
EcoRII CCWGG 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 321
EcoT22I ATGCAT 2 cut(s) 144, 267
ErhI CCWWGG 1 cut(s) 321
FaeI CATG 3 cut(s) 98, 146, 269
FaiI YATR 9 cut(s) 7, 96, 133, 140, 144, 258, 263, 267, 367
FatI CATG 3 cut(s) 94, 142, 265
FbaI TGATCA 1 cut(s) 436
FblI GTMKAC 1 cut(s) 366
Fnu4HI GCNGC 1 cut(s) 107
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 1 cut(s) 107
GluI GCNGC 1 cut(s) 107
Hin1II CATG 3 cut(s) 98, 146, 269
HincII GTYRAC 1 cut(s) 288
HindII GTYRAC 1 cut(s) 288
HinfI GANTC 1 cut(s) 302
Hpy166II GTNNAC 4 cut(s) 27, 218, 288, 367
Hpy188I TCNGA 4 cut(s) 301, 331, 390, 441
Hpy188III TCNNGA 2 cut(s) 160, 460
Hpy8I GTNNAC 4 cut(s) 27, 218, 288, 367
HpyAV CCTTC 2 cut(s) 329, 420
HpyCH4IV ACGT 1 cut(s) 214
HpyCH4V TGCA 2 cut(s) 142, 265
HpyF10VI GCNNNNNNNGC 1 cut(s) 271
HpyF3I CTNAG 2 cut(s) 348, 423
HpySE526I ACGT 1 cut(s) 214
Hsp92II CATG 3 cut(s) 98, 146, 269
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 2 cut(s) 406, 436
LmnI GCTCC 3 cut(s) 114, 189, 194
LpnPI CCDG 7 cut(s) 44, 98, 125, 192, 206, 213, 445
Lsp1109I GCAGC 1 cut(s) 93
LweI GCATC 1 cut(s) 384
MaeII ACGT 1 cut(s) 214
MaeIII GTNAC 1 cut(s) 202
MalI GATC 2 cut(s) 408, 438
MbiI CCGCTC 1 cut(s) 192
MboI GATC 2 cut(s) 406, 436
MboII GAAGA 1 cut(s) 297
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 1 cut(s) 199
MluCI AATT 3 cut(s) 43, 248, 464
MnlI CCTC 2 cut(s) 343, 355
Mph1103I ATGCAT 2 cut(s) 144, 267
MseI TTAA 2 cut(s) 75, 429
MslI CAYNNNNRTG 1 cut(s) 184
MspR9I CCNGG 1 cut(s) 113
MvaI CCWGG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 271
NdeII GATC 2 cut(s) 406, 436
NlaIII CATG 3 cut(s) 98, 146, 269
NlaIV GGNNCC 1 cut(s) 29
NsiI ATGCAT 2 cut(s) 144, 267
NspI RCATGY 1 cut(s) 146
OliI CACNNNNGTG 1 cut(s) 184
PaeR7I CTCGAG 1 cut(s) 182
PfeI GAWTC 1 cut(s) 302
PfoI TCCNGGA 1 cut(s) 111
PkrI GCNGC 1 cut(s) 108
PshAI GACNNNNGTC 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 111
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 1 cut(s) 29
PspPI GGNCC 1 cut(s) 27
PspXI VCTCGAGB 1 cut(s) 182
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 3 cut(s) 56, 278, 421
RsaNI GTAC 3 cut(s) 55, 277, 420
RseI CAYNNNNRTG 1 cut(s) 184
SaqAI TTAA 2 cut(s) 75, 429
SatI GCNGC 1 cut(s) 107
Sau3AI GATC 2 cut(s) 406, 436
Sau96I GGNCC 1 cut(s) 27
ScaI AGTACT 1 cut(s) 56
ScrFI CCNGG 1 cut(s) 113
SduI GDGCHC 1 cut(s) 199
SetI ASST 6 cut(s) 202, 217, 225, 340, 354, 366
SfaNI GCATC 1 cut(s) 384
Sfr274I CTCGAG 1 cut(s) 182
SinI GGWCC 1 cut(s) 27
SlaI CTCGAG 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
Sse9I AATT 3 cut(s) 43, 248, 464
SsiI CCGC 1 cut(s) 192
StyD4I CCNGG 1 cut(s) 111
StyI CCWWGG 1 cut(s) 321
TaiI ACGT 1 cut(s) 217
TaqI TCGA 2 cut(s) 183, 413
TasI AATT 3 cut(s) 43, 248, 464
TatI WGTACW 3 cut(s) 54, 276, 419
TfiI GAWTC 1 cut(s) 302
Tru1I TTAA 2 cut(s) 75, 429
Tru9I TTAA 2 cut(s) 75, 429
TseI GCWGC 1 cut(s) 106
TspDTI ATGAA 2 cut(s) 348, 363
TspGWI ACGGA 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 2 cut(s) 43, 248
XceI RCATGY 1 cut(s) 146
XhoI CTCGAG 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 366
ZrmI AGTACT 1 cut(s) 56
Zsp2I ATGCAT 2 cut(s) 144, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.