Rh2AG255200

DNA-directed RNA polymerases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0015704
Physical Location & Seq
Forward (+)
29092828 .. 29095500
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG255200.1

Sequence Viewer

Length: 381 bp
ATGCTGAGTTTCTTGTTTGATGATACTTTTGTCATCGAGAAGCTGGACCCGGATGGTAAAAAGTTTGATAAAGTTTCCAGAATTGTAGCACGGAGTGAGAAGCATGGTGCTTTGCTGCACCTGGATGTGAATTCGGAGATATACCCTATGCATGAGAAGGATAAATTTTTGATGGTGCTGTCTCCCACACTTAACTACAGTGGAGCACCTGTTACGAACCAGGCACAGGTTGAACAGAAGTCTCTCGCAGACAAGTTTGAATATATCATGCATGGTTTGCTGTATAAAATGGATCACATGAGATCAGAACCTTCAGATGGAGGCTCAAAGTCACCACCCTTAGTGTATGTTTCATACTGCTCAAATTATATTTTCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05016 ko05169 map00230 map00240 map01100 map03020 map04623 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb8

Protein Analysis

126

Amino Acids

14.4

Weight (kDa)

5.91

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb8 PF03870 7 - 103 2.1e-30 RNA polymerase Rpb8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015704)

Species Orthologous Gene IDs
malus_domestica MD01G1023800.v1.1 MD15G1329500.v1.1
prunus_persica Prupe.6G178800_v2.0.a1
pyrus_communis pycom01g05140 pycom15g29600
rosa_chinensis RchiOBHm_Chr2g0115551
rosa_laevigata RLG00000018193
rosa_multiflora Rmu_ssc0000197.1_g000090
rosa_roxburghii Rroxscaffold_2G00128110
rosa_rugosa Rorug02G0197900 Rorug02G0198000
rosa_samantha Rh2AG255200 Rh2BG265900 Rh2CG260400 Rh2DG263400
rosa_wichuraiana Rw2G019980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 300
AcsI RAATTY 2 cut(s) 130, 164
AcuI CTGAAG 1 cut(s) 297
AdeI CACNNNGTG 1 cut(s) 95
AfiI CCNNNNNNNGG 2 cut(s) 226, 317
AgsI TTSAA 2 cut(s) 233, 260
AjnI CCWGG 2 cut(s) 120, 219
AluBI AGCT 1 cut(s) 43
AluI AGCT 1 cut(s) 43
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 2 cut(s) 186, 246
AlwI GGATC 1 cut(s) 300
ApeKI GCWGC 1 cut(s) 115
ApoI RAATTY 2 cut(s) 130, 164
AspS9I GGNCC 1 cut(s) 46
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 1 cut(s) 324
AvaII GGWCC 1 cut(s) 46
BaeI ACNNNNGTAYC 2 cut(s) 15, 48
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 1 cut(s) 102
BccI CCATC 3 cut(s) 47, 166, 311
BciT130I CCWGG 2 cut(s) 122, 221
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 2 cut(s) 186, 246
BfmI CTRYAG 1 cut(s) 196
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
Bme1390I CCNGG 3 cut(s) 50, 122, 221
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 3 cut(s) 50, 122, 221
BpuMI CCSGG 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 317
BseBI CCWGG 2 cut(s) 122, 221
BseGI GGATG 2 cut(s) 58, 130
BseLI CCNNNNNNNGG 2 cut(s) 226, 317
BseXI GCAGC 1 cut(s) 102
BsgI GTGCAG 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 208
BsiSI CCGG 1 cut(s) 50
BslI CCNNNNNNNGG 2 cut(s) 226, 317
BsmAI GTCTC 2 cut(s) 186, 246
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 2 cut(s) 292, 302
BspLI GGNNCC 1 cut(s) 48
BspPI GGATC 1 cut(s) 300
BssMI GATC 2 cut(s) 292, 302
Bst2UI CCWGG 2 cut(s) 122, 221
Bst4CI ACNGT 1 cut(s) 200
BstAPI GCANNNNNTGC 1 cut(s) 277
BstDEI CTNAG 2 cut(s) 5, 340
BstF5I GGATG 2 cut(s) 58, 130
BstKTI GATC 2 cut(s) 295, 305
BstMAI GTCTC 2 cut(s) 186, 246
BstMBI GATC 2 cut(s) 292, 302
BstMWI GCNNNNNNNGC 1 cut(s) 277
BstNI CCWGG 2 cut(s) 122, 221
BstSCI CCNGG 3 cut(s) 48, 120, 219
BstSFI CTRYAG 1 cut(s) 196
BstV1I GCAGC 1 cut(s) 102
BtsCI GGATG 2 cut(s) 58, 130
BtsIMutI CAGTG 1 cut(s) 205
Cfr13I GGNCC 1 cut(s) 46
CviAII CATG 5 cut(s) 104, 152, 268, 272, 298
CviJI RGCY 2 cut(s) 43, 324
CviKI_1 RGCY 2 cut(s) 43, 324
DdeI CTNAG 2 cut(s) 5, 340
DpnI GATC 2 cut(s) 294, 304
DpnII GATC 2 cut(s) 292, 302
DraIII CACNNNGTG 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 297
EcoRI GAATTC 1 cut(s) 130
EcoRII CCWGG 2 cut(s) 120, 219
EcoT22I ATGCAT 2 cut(s) 153, 273
FaeI CATG 5 cut(s) 107, 155, 271, 275, 301
FatI CATG 5 cut(s) 103, 151, 267, 271, 297
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 2 cut(s) 65, 137
Fsp4HI GCNGC 1 cut(s) 116
GluI GCNGC 1 cut(s) 116
HapII CCGG 1 cut(s) 50
Hin1II CATG 5 cut(s) 107, 155, 271, 275, 301
HpaII CCGG 1 cut(s) 50
HphI GGTGA 1 cut(s) 324
Hpy188I TCNGA 3 cut(s) 136, 307, 316
Hpy188III TCNNGA 2 cut(s) 37, 78
HpyAV CCTTC 2 cut(s) 151, 321
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4V TGCA 3 cut(s) 118, 151, 271
HpyF10VI GCNNNNNNNGC 1 cut(s) 277
HpyF3I CTNAG 2 cut(s) 5, 340
Hsp92II CATG 5 cut(s) 107, 155, 271, 275, 301
Kzo9I GATC 2 cut(s) 292, 302
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 9 cut(s) 29, 63, 91, 107, 134, 206, 212, 222, 233
Lsp1109I GCAGC 1 cut(s) 102
MaeIII GTNAC 2 cut(s) 211, 330
MalI GATC 2 cut(s) 294, 304
MboI GATC 2 cut(s) 292, 302
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 4 cut(s) 81, 130, 164, 364
MnlI CCTC 1 cut(s) 314
Mph1103I ATGCAT 2 cut(s) 153, 273
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 123
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 3 cut(s) 50, 122, 221
MvaI CCWGG 2 cut(s) 122, 221
MwoI GCNNNNNNNGC 1 cut(s) 277
NciI CCSGG 1 cut(s) 50
NdeII GATC 2 cut(s) 292, 302
NlaIII CATG 5 cut(s) 107, 155, 271, 275, 301
NlaIV GGNNCC 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 330
NsiI ATGCAT 2 cut(s) 153, 273
PkrI GCNGC 1 cut(s) 117
Psp6I CCWGG 2 cut(s) 120, 219
PspGI CCWGG 2 cut(s) 120, 219
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 123
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 2 cut(s) 292, 302
Sau96I GGNCC 1 cut(s) 46
ScrFI CCNGG 3 cut(s) 50, 122, 221
SduI GDGCHC 1 cut(s) 208
SetI ASST 5 cut(s) 45, 123, 211, 231, 313
SfcI CTRYAG 1 cut(s) 196
SinI GGWCC 1 cut(s) 46
SmiMI CAYNNNNRTG 1 cut(s) 123
Sse9I AATT 4 cut(s) 81, 130, 164, 364
StyD4I CCNGG 3 cut(s) 48, 120, 219
TaaI ACNGT 1 cut(s) 200
TaqI TCGA 1 cut(s) 36
TasI AATT 4 cut(s) 81, 130, 164, 364
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 1 cut(s) 330
TseI GCWGC 1 cut(s) 115
Tsp45I GTSAC 1 cut(s) 330
TspDTI ATGAA 1 cut(s) 342
TspGWI ACGGA 1 cut(s) 106
TspRI CASTG 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 2 cut(s) 130, 164
Zsp2I ATGCAT 2 cut(s) 153, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.