Rorug02G0197900

DNA-directed RNA polymerases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015704
Physical Location & Seq
Forward (+)
18445764 .. 18446694
931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0197900.1

Sequence Viewer

Length: 615 bp
ATGCAAACTACTAGCATATTCACAACTCCCACATCTCAAACTTCAACTCAATCCCGCTCACAGAAAACATCCAAATCCACAACAATGGAAGCTGCTTACACTTCATCCTCTTCAATTACCTCCAAGCAAGCACCACTGCCTTACCACCACTCCTCACTCCACTCCGTCAAGAAAAACTCAGCCAAGCCGTGGCGAAAGCCGGCAGTGGCGCCGCTCCCTCCGACCCCGCCACGAGTGTACAAAGTCGACCCCATCAACTTTCGTGACCTTGTCCAGAAGCTCACCAGTGCACCCGAGTACCTGAACGAGCCGACTCGGTCGACTAACCTCCAAAGCGTGGCGCCTCCACCGATCGAAATCCAACGAGGACCACCACATGTTCCTTCTCCGGTTTCCGCTAAGGTTACTGGCAGCACTTCGCCTTTTTCAGTTATGTACAAGGACTTGGCTGAGACATTAGGGCTGAGTAGTACTCCTCATCATCAGAAGAAGGCAGTGGATCAAAACATCACGGGGGACTCGAGCTATCTGCGGTTGAACTTGTCGCCGGCTTCGCAGCATTGGTTCTCTTTTCCTATGTTGAGCCCTGGAACTCTGACCAGTCTGGAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05016 ko05169 map00230 map00240 map01100 map03020 map04623 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb8

Protein Analysis

204

Amino Acids

22.25

Weight (kDa)

9.86

Isoelectric Point (pI)

57.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 75 - 95 7e-08 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015704)

Species Orthologous Gene IDs
malus_domestica MD01G1023800.v1.1 MD15G1329500.v1.1
prunus_persica Prupe.6G178800_v2.0.a1
pyrus_communis pycom01g05140 pycom15g29600
rosa_chinensis RchiOBHm_Chr2g0115551
rosa_laevigata RLG00000018193
rosa_multiflora Rmu_ssc0000197.1_g000090
rosa_roxburghii Rroxscaffold_2G00128110
rosa_rugosa Rorug02G0197900 Rorug02G0198000
rosa_samantha Rh2AG255200 Rh2BG265900 Rh2CG260400 Rh2DG263400
rosa_wichuraiana Rw2G019980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 208, 340
AccB7I CCANNNNNTGG 2 cut(s) 189, 337
AccBSI CCGCTC 2 cut(s) 57, 214
AccI GTMKAC 2 cut(s) 246, 320
AciI CCGC 5 cut(s) 55, 212, 227, 396, 532
AclWI GGATC 1 cut(s) 507
AcyI GRCGYC 2 cut(s) 209, 341
AfaI GTAC 4 cut(s) 239, 299, 437, 472
AfiI CCNNNNNNNGG 2 cut(s) 189, 337
AflIII ACRYGT 1 cut(s) 376
AgsI TTSAA 3 cut(s) 45, 114, 538
AjnI CCWGG 1 cut(s) 586
AluBI AGCT 3 cut(s) 92, 280, 525
AluI AGCT 3 cut(s) 92, 280, 525
Alw21I GWGCWC 1 cut(s) 292
Alw26I GTCTC 1 cut(s) 446
Alw44I GTGCAC 1 cut(s) 288
AlwI GGATC 1 cut(s) 507
Ama87I CYCGRG 2 cut(s) 293, 520
ApaLI GTGCAC 1 cut(s) 288
ApeKI GCWGC 3 cut(s) 92, 411, 556
AspLEI GCGC 2 cut(s) 211, 343
AspS9I GGNCC 1 cut(s) 368
AsuHPI GGTGA 1 cut(s) 274
AvaI CYCGRG 2 cut(s) 293, 520
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 292
BaeI ACNNNNGTAYC 2 cut(s) 281, 314
BanI GGYRCC 2 cut(s) 208, 340
BanII GRGCYC 1 cut(s) 587
BauI CACGAG 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 292
BbvI GCAGC 3 cut(s) 79, 423, 568
BccI CCATC 1 cut(s) 260
BceAI ACGGC 1 cut(s) 172
BcgI CGANNNNNNTGC 2 cut(s) 511, 545
BciT130I CCWGG 1 cut(s) 588
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 2 cut(s) 12, 613
BfoI RGCGCY 2 cut(s) 212, 344
BisI GCNGC 4 cut(s) 93, 212, 412, 557
BlsI GCNGC 4 cut(s) 94, 213, 413, 558
BmcAI AGTACT 1 cut(s) 472
Bme1390I CCNGG 1 cut(s) 588
Bme18I GGWCC 1 cut(s) 368
BmeT110I CYCGRG 2 cut(s) 293, 520
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 2 cut(s) 210, 342
BmrFI CCNGG 1 cut(s) 588
BplI GAGNNNNNCTC 2 cut(s) 457, 489
Bpu10I CCTNAGC 1 cut(s) 399
BsaBI GATNNNNATC 1 cut(s) 356
BsaHI GRCGYC 2 cut(s) 209, 341
BsaJI CCNNGG 2 cut(s) 188, 586
BsaWI WCCGGW 1 cut(s) 388
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 337
Bse118I RCCGGY 2 cut(s) 199, 547
Bse1I ACTGG 3 cut(s) 285, 412, 600
Bse8I GATNNNNATC 1 cut(s) 356
BseBI CCWGG 1 cut(s) 588
BseDI CCNNGG 2 cut(s) 188, 586
BseGI GGATG 2 cut(s) 68, 104
BseJI GATNNNNATC 1 cut(s) 356
BseLI CCNNNNNNNGG 2 cut(s) 189, 337
BseMII CTCAG 3 cut(s) 192, 441, 455
BseNI ACTGG 3 cut(s) 285, 412, 600
BseRI GAGGAG 2 cut(s) 142, 465
BseSI GKGCMC 1 cut(s) 292
BseXI GCAGC 3 cut(s) 79, 423, 568
Bsh1285I CGRYCG 2 cut(s) 320, 354
BshNI GGYRCC 2 cut(s) 208, 340
BsiEI CGRYCG 2 cut(s) 320, 354
BsiHKAI GWGCWC 1 cut(s) 292
BsiHKCI CYCGRG 2 cut(s) 293, 520
BsiSI CCGG 3 cut(s) 200, 389, 548
BslFI GGGAC 1 cut(s) 530
BslI CCNNNNNNNGG 2 cut(s) 189, 337
BsmAI GTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 530
BsoBI CYCGRG 2 cut(s) 293, 520
Bsp1286I GDGCHC 2 cut(s) 292, 587
Bsp1407I TGTACA 2 cut(s) 237, 435
Bsp143I GATC 2 cut(s) 351, 499
BspACI CCGC 5 cut(s) 55, 212, 227, 396, 532
BspCNI CTCAG 3 cut(s) 191, 442, 456
BspLI GGNNCC 2 cut(s) 210, 342
BspPI GGATC 1 cut(s) 507
BspT107I GGYRCC 2 cut(s) 208, 340
BsrBI CCGCTC 2 cut(s) 57, 214
BsrFI RCCGGY 2 cut(s) 199, 547
BsrGI TGTACA 2 cut(s) 237, 435
BsrI ACTGG 3 cut(s) 285, 412, 600
BssAI RCCGGY 2 cut(s) 199, 547
BssECI CCNNGG 2 cut(s) 188, 586
BssMI GATC 2 cut(s) 351, 499
BssNI GRCGYC 2 cut(s) 209, 341
BssSI CACGAG 1 cut(s) 231
Bst2BI CACGAG 1 cut(s) 231
Bst2UI CCWGG 1 cut(s) 588
Bst6I CTCTTC 1 cut(s) 115
BstACI GRCGYC 2 cut(s) 209, 341
BstAUI TGTACA 2 cut(s) 237, 435
BstC8I GCNNGC 3 cut(s) 129, 201, 549
BstDEI CTNAG 4 cut(s) 178, 399, 450, 464
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 2 cut(s) 68, 104
BstH2I RGCGCY 2 cut(s) 212, 344
BstHHI GCGC 2 cut(s) 211, 343
BstKTI GATC 2 cut(s) 354, 502
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 2 cut(s) 351, 499
BstMCI CGRYCG 2 cut(s) 320, 354
BstMWI GCNNNNNNNGC 1 cut(s) 553
BstNI CCWGG 1 cut(s) 588
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 1 cut(s) 586
BstSLI GKGCMC 1 cut(s) 292
BstV1I GCAGC 3 cut(s) 79, 423, 568
BstXI CCANNNNNNTGG 1 cut(s) 85
BtgI CCRYGG 1 cut(s) 188
BtsCI GGATG 2 cut(s) 68, 104
BtsI GCAGTG 3 cut(s) 134, 210, 501
BtsIMutI CAGTG 4 cut(s) 134, 210, 292, 501
Cac8I GCNNGC 3 cut(s) 129, 201, 549
CfoI GCGC 2 cut(s) 211, 343
Cfr10I RCCGGY 2 cut(s) 199, 547
Cfr13I GGNCC 1 cut(s) 368
Csp6I GTAC 4 cut(s) 238, 298, 436, 471
CviAII CATG 1 cut(s) 377
CviQI GTAC 4 cut(s) 238, 298, 436, 471
DdeI CTNAG 4 cut(s) 178, 399, 450, 464
DinI GGCGCC 2 cut(s) 210, 342
DpnI GATC 2 cut(s) 353, 501
DpnII GATC 2 cut(s) 351, 499
Eam1104I CTCTTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 587
Eco47I GGWCC 1 cut(s) 368
Eco88I CYCGRG 2 cut(s) 293, 520
EcoRII CCWGG 1 cut(s) 586
EcoT38I GRGCYC 1 cut(s) 587
EgeI GGCGCC 2 cut(s) 210, 342
EheI GGCGCC 2 cut(s) 210, 342
FaeI CATG 1 cut(s) 380
FaiI YATR 4 cut(s) 17, 378, 434, 578
FaqI GGGAC 1 cut(s) 530
FatI CATG 1 cut(s) 376
FauI CCCGC 2 cut(s) 62, 234
FblI GTMKAC 2 cut(s) 246, 320
Fnu4HI GCNGC 4 cut(s) 93, 212, 412, 557
FokI GGATG 2 cut(s) 55, 91
FriOI GRGCYC 1 cut(s) 587
Fsp4HI GCNGC 4 cut(s) 93, 212, 412, 557
FspBI CTAG 2 cut(s) 12, 613
GlaI GCGC 2 cut(s) 210, 342
GluI GCNGC 4 cut(s) 93, 212, 412, 557
HaeII RGCGCY 2 cut(s) 212, 344
HapII CCGG 3 cut(s) 200, 389, 548
HhaI GCGC 2 cut(s) 211, 343
Hin1I GRCGYC 2 cut(s) 209, 341
Hin1II CATG 1 cut(s) 380
Hin6I GCGC 2 cut(s) 209, 341
HinP1I GCGC 2 cut(s) 209, 341
HincII GTYRAC 2 cut(s) 247, 321
HindII GTYRAC 2 cut(s) 247, 321
HinfI GANTC 2 cut(s) 313, 518
HpaII CCGG 3 cut(s) 200, 389, 548
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 4 cut(s) 238, 247, 290, 321
Hpy188I TCNGA 3 cut(s) 222, 486, 597
Hpy188III TCNNGA 4 cut(s) 169, 263, 274, 605
Hpy8I GTNNAC 4 cut(s) 238, 247, 290, 321
HpyAV CCTTC 2 cut(s) 393, 484
HpyCH4V TGCA 2 cut(s) 4, 290
HpyF10VI GCNNNNNNNGC 1 cut(s) 553
HpyF3I CTNAG 4 cut(s) 178, 399, 450, 464
Hsp92I GRCGYC 2 cut(s) 209, 341
Hsp92II CATG 1 cut(s) 380
HspAI GCGC 2 cut(s) 209, 341
KasI GGCGCC 2 cut(s) 208, 340
KroI GCCGGC 2 cut(s) 199, 547
KroNI GCCGGC 2 cut(s) 201, 549
Kzo9I GATC 2 cut(s) 351, 499
LmnI GCTCC 1 cut(s) 219
Lsp1109I GCAGC 3 cut(s) 79, 423, 568
MaeI CTAG 2 cut(s) 12, 613
MaeIII GTNAC 2 cut(s) 263, 403
MalI GATC 2 cut(s) 353, 501
MbiI CCGCTC 2 cut(s) 57, 214
MboI GATC 2 cut(s) 351, 499
MboII GAAGA 2 cut(s) 102, 499
MhlI GDGCHC 2 cut(s) 292, 587
MluCI AATT 1 cut(s) 114
Mly113I GGCGCC 2 cut(s) 209, 341
MlyI GAGTC 2 cut(s) 307, 512
MmeI TCCRAC 2 cut(s) 245, 385
MnlI CCTC 8 cut(s) 118, 130, 163, 228, 338, 354, 359, 486
MroNI GCCGGC 2 cut(s) 199, 547
MslI CAYNNNNRTG 1 cut(s) 83
MspI CCGG 3 cut(s) 200, 389, 548
MspR9I CCNGG 1 cut(s) 588
MvaI CCWGG 1 cut(s) 588
MwoI GCNNNNNNNGC 1 cut(s) 553
NaeI GCCGGC 2 cut(s) 201, 549
NarI GGCGCC 2 cut(s) 209, 341
NdeII GATC 2 cut(s) 351, 499
NgoMIV GCCGGC 2 cut(s) 199, 547
NlaIII CATG 1 cut(s) 380
NlaIV GGNNCC 2 cut(s) 210, 342
NmuCI GTSAC 1 cut(s) 263
NspI RCATGY 1 cut(s) 380
PaeR7I CTCGAG 1 cut(s) 520
PciI ACATGT 1 cut(s) 376
PcsI WCGNNNNNNNCGW 1 cut(s) 518
PdiI GCCGGC 2 cut(s) 201, 549
PflFI GACNNNGTC 2 cut(s) 269, 316
PflMI CCANNNNNTGG 2 cut(s) 189, 337
PkrI GCNGC 4 cut(s) 94, 213, 413, 558
Ple19I CGATCG 1 cut(s) 354
PleI GAGTC 2 cut(s) 307, 512
PluTI GGCGCC 2 cut(s) 212, 344
PpsI GAGTC 2 cut(s) 307, 512
PscI ACATGT 1 cut(s) 376
Psp6I CCWGG 1 cut(s) 586
PspGI CCWGG 1 cut(s) 586
PspN4I GGNNCC 2 cut(s) 210, 342
PspPI GGNCC 1 cut(s) 368
PspXI VCTCGAGB 1 cut(s) 520
PsyI GACNNNGTC 2 cut(s) 269, 316
PvuI CGATCG 1 cut(s) 354
RsaI GTAC 4 cut(s) 239, 299, 437, 472
RsaNI GTAC 4 cut(s) 238, 298, 436, 471
RseI CAYNNNNRTG 1 cut(s) 83
SalI GTCGAC 2 cut(s) 245, 319
SatI GCNGC 4 cut(s) 93, 212, 412, 557
Sau3AI GATC 2 cut(s) 351, 499
Sau96I GGNCC 1 cut(s) 368
ScaI AGTACT 1 cut(s) 472
SchI GAGTC 2 cut(s) 307, 512
ScrFI CCNGG 1 cut(s) 588
SduI GDGCHC 2 cut(s) 292, 587
SetI ASST 8 cut(s) 94, 122, 270, 282, 303, 330, 405, 527
SfoI GGCGCC 2 cut(s) 210, 342
Sfr274I CTCGAG 1 cut(s) 520
SinI GGWCC 1 cut(s) 368
SlaI CTCGAG 1 cut(s) 520
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
Sse9I AATT 1 cut(s) 114
SsiI CCGC 5 cut(s) 55, 212, 227, 396, 532
SspDI GGCGCC 2 cut(s) 208, 340
SspMI CTAG 2 cut(s) 12, 613
StyD4I CCNGG 1 cut(s) 586
TaqI TCGA 4 cut(s) 246, 320, 354, 521
TaqII GACCGA 1 cut(s) 306
TasI AATT 1 cut(s) 114
TatI WGTACW 3 cut(s) 237, 435, 470
TauI GCSGC 1 cut(s) 214
TscAI CASTG 4 cut(s) 141, 210, 292, 501
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 3 cut(s) 92, 411, 556
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 1 cut(s) 93
TspGWI ACGGA 1 cut(s) 154
TspRI CASTG 4 cut(s) 141, 210, 292, 501
Tth111I GACNNNGTC 2 cut(s) 269, 316
Van91I CCANNNNNTGG 2 cut(s) 189, 337
VneI GTGCAC 1 cut(s) 288
VpaK11BI GGWCC 1 cut(s) 368
XceI RCATGY 1 cut(s) 380
XhoI CTCGAG 1 cut(s) 520
XmiI GTMKAC 2 cut(s) 246, 320
XspI CTAG 2 cut(s) 12, 613
ZrmI AGTACT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.