pycom15g39080

Protein NRT1 PTR FAMILY 4.3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009913
Physical Location & Seq
Forward (+)
38776033 .. 38779505
3473 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1839 bp
ATGGAAATGGAACAAAGGAGGCAGAATCACAAAGTTGAGGCCATTAACACATCTGACATGGCTGAAGAGATGACTGTTGATTGGAGGGGCAGACCTTCCAATCCTACCAAGCATGGTGGCATGAGAGCTGCTGCATTTGTTCTTGGGCTTCAATCATTTGAGATAATGGGAATTGCTGCAGTTGGGAACAACCTCATAACATACGTGATAAATGAGATGCATTTTCCTTTGTCGAATTCGGCCAACGTAGTGACAAACTTTATTGGAACTGTCTTTCTCTTAGCCCTTGTTGGTGGCTACCTCTCCGATTCCTATCTTGGGAGTTTCTGGACCATGCTTATCTTTGGTTTTGTGGAACTTTCGGGTTTCATATTACTGTCAGTCCAAGCTCATGTTCCGCAGCTAAAGCCACCGCCATGCAACGTTAATAACGGAGAGGAGTGTGTAGAGGCAAAAGGGTTCAAGTCATTAATCTTCTTTGTGGCACTTTACTTGGTGGCATTGGGGAGTGGCTGTGTGAAGCCAAACATGATCGCTCACGGGGCTGACCAGTTCAACCCAGATGACCCGAAGCAGTCCAAGAAACTCTCCACCTACTTCAACGCTGCCTACTTTGCCTTTTCCATGGGTGAACTCGTCGCCCTCACTCTTCTTGTGTGGGTCCAAACTCACTCTGGAATGGATGTTGGCTTTGGAGTCTCAGCAGCCGCCATGGCCATGGGATTAATCAGCTTGGTTTCCGGTACCCTATATTACAGGAACAAGCGACCTCAAGGAAGCATACTCACTCCCATTGCTCAAGTTTTTGTGGCTGCAATGCTAAAGAGAAAGCAGGCTTGTCCATCTAATCCACAGTTGCTCCATGGAAACCAAAACAATGTGCTGCATGACAATACTACTTTCTTTTCTGACGCTGCCAGCTTTCTTCACACTGAAAAGTTCAGGTTCTTGGACAAGGCTTGCATTAAAATTCAAGATGGCACTAATTCAAAGGAAAGCCCGTGGAGATTGTGCACCGTTGCTCAAGTGAAGCAAGTCAAAATACTGCTTTCAGTTATTCCAATTTTTGCTTCCACCATAATTTTCAACACAATTTTGGCTCAGCTCCAAACTTTCTCAGTCCAACAAGGAAGTATGATGGACACCAAGCTTACCAAATCCTTCCATATCCCTCCAGCTTCACTCCAATCCATCCCTTACATCATTCTCATCGTCCTCGTCCCTCTCTACGACACTTTCTTTGTTCCCTTTGCTCGAAAATTCACCGGTCACGATTCTGGCATCTCTCCATTAATGAGAATAGGTTTTGGACTCTTTTTTGCAACTTTTTCGATGATCGCGGCTGCCATCATGGAGGAAAAGAGAAGGGATGCAGCTGTGCATTCGAACAAAATAATTTCCATTTTTTGGATCACCCCACAATTCTTGATCTTTGGATTATCAGAAATGTTCACTGCCGTAGGCCTCATTGAGTTCTTTTACAAGCAATCCTTGAAGGGGATGCAAGCATTTTTAACTGCCTTGACCTATTGCTCATATTCGTTTGGGTTTTACCTGAGCTCTCTACTTGTCTCTCTGGTGAATAAGATCACAACCTCATCAGGTGGTGGTGGTTGGCTGGGTGACAATAATCTCAACAAAGACAGACTGGACCTTTTCTATTGGTTGCTGGCAGGCCTCAGCTTCCTCAACTTCCTCAACTATCTATTTTGGTCTCATTGGTACTCTCACACCCCGTCCTCTTCATCAGCACCAAGTGCGCTACCCCAGCTGCATGCTCATGATACTACTTGCATGGAGGATTATAAACATCATAGACTGGATGATAATATACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0016020 GO:0043207 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

613

Amino Acids

67.96

Weight (kDa)

8.28

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 109 - 539 9.2e-92 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G59740
fragaria_vesca FvH4_5g27820 FvH4_5g27820 FvH4_5g27820
malus_domestica MD08G1248200.v1.1 MD15G1443100.v1.1
prunus_persica Prupe.1G585400_v2.0.a1
pyrus_communis pycom08g21500 pycom15g39080
rosa_chinensis RchiOBHm_Chr7g0219651
rosa_laevigata RLG00000002280
rosa_multiflora Rmu_sc0001828.1_g000003
rosa_roxburghii Rroxscaffold_3G00239960
rosa_rugosa Rorug07G0186700
rosa_samantha Rh1BG165700 Rh1DG196300 Rh7AG330500 Rh7BG320300 Rh7CG347800 Rh7DG325800
rosa_wichuraiana Rw7G027820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1806
Acc65I GGTACC 1 cut(s) 743
AccB1I GGYRCC 1 cut(s) 743
AccB7I CCANNNNNTGG 1 cut(s) 1407
AccII CGCG 1 cut(s) 1340
AciI CCGC 4 cut(s) 398, 413, 708, 1340
AclI AACGTT 1 cut(s) 423
AclWI GGATC 1 cut(s) 1418
AcoI YGGCCR 2 cut(s) 240, 714
AcsI RAATTY 3 cut(s) 235, 969, 1259
AcuI CTGAAG 1 cut(s) 84
AdeI CACNNNGTG 1 cut(s) 1757
AfaI GTAC 2 cut(s) 745, 1724
AfiI CCNNNNNNNGG 3 cut(s) 318, 1407, 1497
AgeI ACCGGT 1 cut(s) 1265
AgsI TTSAA 8 cut(s) 152, 463, 556, 601, 974, 990, 1087, 1495
AjuI GAANNNNNNNTTGG 4 cut(s) 378, 410, 1054, 1086
Alw21I GWGCWC 2 cut(s) 1016, 1562
Alw26I GTCTC 3 cut(s) 703, 1576, 1719
Alw44I GTGCAC 1 cut(s) 1012
AlwI GGATC 1 cut(s) 1418
AoxI GGCC 5 cut(s) 39, 240, 714, 1462, 1675
ApaLI GTGCAC 1 cut(s) 1012
ApoI RAATTY 3 cut(s) 235, 969, 1259
ArsI GACNNNNNNTTYG 2 cut(s) 1223, 1255
AseI ATTAAT 3 cut(s) 470, 725, 1292
AsiGI ACCGGT 1 cut(s) 1265
Asp718I GGTACC 1 cut(s) 743
AspLEI GCGC 1 cut(s) 1762
AspS9I GGNCC 3 cut(s) 330, 661, 1651
AsuHPI GGTGA 5 cut(s) 641, 1255, 1405, 1591, 1634
AsuII TTCGAA 1 cut(s) 1385
AvaII GGWCC 3 cut(s) 330, 661, 1651
BaeGI GKGCMC 1 cut(s) 1016
BalI TGGCCA 1 cut(s) 716
BanI GGYRCC 1 cut(s) 743
BanII GRGCYC 1 cut(s) 1562
Bbv12I GWGCWC 2 cut(s) 1016, 1562
BbvCI CCTCAGC 1 cut(s) 1679
BccI CCATC 5 cut(s) 850, 971, 1132, 1199, 1355
BceAI ACGGC 1 cut(s) 1442
BcgI CGANNNNNNTGC 2 cut(s) 1311, 1345
BcoDI GTCTC 3 cut(s) 703, 1576, 1719
BfmI CTRYAG 1 cut(s) 177
BglI GCCNNNNNGGC 1 cut(s) 713
BlpI GCTNAGC 1 cut(s) 1101
Bme18I GGWCC 3 cut(s) 330, 661, 1651
BmgT120I GGNCC 3 cut(s) 330, 661, 1651
BmiI GGNNCC 2 cut(s) 662, 745
BmsI GCATC 4 cut(s) 207, 1290, 1360, 1491
BpmI CTGGAG 1 cut(s) 1158
Bpu10I CCTNAGC 2 cut(s) 1556, 1679
Bpu1102I GCTNAGC 1 cut(s) 1101
Bpu14I TTCGAA 1 cut(s) 1385
BpuEI CTTGAG 3 cut(s) 756, 783, 1008
BsaAI YACGTR 1 cut(s) 205
BsaBI GATNNNNATC 1 cut(s) 312
BsaI GGTCTC 1 cut(s) 1719
BsaJI CCNNGG 5 cut(s) 624, 711, 717, 862, 1001
BsaWI WCCGGW 2 cut(s) 740, 1265
BsaXI ACNNNNNCTCC 4 cut(s) 499, 529, 843, 873
Bsc4I CCNNNNNNNGG 3 cut(s) 318, 1407, 1497
Bse118I RCCGGY 1 cut(s) 1265
Bse1I ACTGG 3 cut(s) 550, 1653, 1824
Bse3DI GCAATG 2 cut(s) 792, 822
Bse8I GATNNNNATC 1 cut(s) 312
BseDI CCNNGG 5 cut(s) 624, 711, 717, 862, 1001
BseGI GGATG 5 cut(s) 688, 1191, 1375, 1506, 1828
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 3 cut(s) 318, 1407, 1497
BseMI GCAATG 2 cut(s) 792, 822
BseMII CTCAG 5 cut(s) 714, 1115, 1131, 1547, 1693
BseNI ACTGG 3 cut(s) 550, 1653, 1824
BseRI GAGGAG 1 cut(s) 452
BseSI GKGCMC 1 cut(s) 1016
BseYI CCCAGC 2 cut(s) 1618, 1767
Bsh1236I CGCG 1 cut(s) 1340
BshFI GGCC 5 cut(s) 41, 242, 716, 1464, 1677
BshNI GGYRCC 1 cut(s) 743
BshTI ACCGGT 1 cut(s) 1265
BsiHKAI GWGCWC 2 cut(s) 1016, 1562
BsiSI CCGG 2 cut(s) 741, 1266
BslFI GGGAC 1 cut(s) 1205
BslI CCNNNNNNNGG 3 cut(s) 318, 1407, 1497
BsmAI GTCTC 3 cut(s) 703, 1576, 1719
BsmFI GGGAC 1 cut(s) 1205
BsmI GAATGC 1 cut(s) 1381
BsnI GGCC 5 cut(s) 41, 242, 716, 1464, 1677
Bso31I GGTCTC 1 cut(s) 1719
Bsp119I TTCGAA 1 cut(s) 1385
Bsp1286I GDGCHC 2 cut(s) 1016, 1562
Bsp143I GATC 5 cut(s) 531, 1335, 1410, 1428, 1587
Bsp1720I GCTNAGC 1 cut(s) 1101
Bsp19I CCATGG 4 cut(s) 624, 711, 717, 862
BspACI CCGC 4 cut(s) 398, 413, 708, 1340
BspANI GGCC 5 cut(s) 41, 242, 716, 1464, 1677
BspCNI CTCAG 5 cut(s) 713, 1114, 1130, 1548, 1692
BspFNI CGCG 1 cut(s) 1340
BspHI TCATGA 1 cut(s) 1780
BspLI GGNNCC 2 cut(s) 662, 745
BspMAI CTGCAG 1 cut(s) 181
BspPI GGATC 1 cut(s) 1418
BspT104I TTCGAA 1 cut(s) 1385
BspT107I GGYRCC 1 cut(s) 743
BspTNI GGTCTC 1 cut(s) 1719
BsrDI GCAATG 2 cut(s) 792, 822
BsrFI RCCGGY 1 cut(s) 1265
BsrI ACTGG 3 cut(s) 550, 1653, 1824
BssAI RCCGGY 1 cut(s) 1265
BssECI CCNNGG 5 cut(s) 624, 711, 717, 862, 1001
BssMI GATC 5 cut(s) 531, 1335, 1410, 1428, 1587
BssT1I CCWWGG 4 cut(s) 624, 711, 717, 862
Bst4CI ACNGT 5 cut(s) 76, 271, 378, 855, 1018
Bst6I CTCTTC 3 cut(s) 60, 654, 1747
BstAPI GCANNNNNTGC 1 cut(s) 1757
BstBAI YACGTR 1 cut(s) 205
BstBI TTCGAA 1 cut(s) 1385
BstC8I GCNNGC 7 cut(s) 834, 919, 961, 1506, 1671, 1675, 1776
BstDEI CTNAG 6 cut(s) 280, 700, 1101, 1117, 1556, 1679
BstDSI CCRYGG 5 cut(s) 624, 711, 717, 862, 1001
BstF5I GGATG 5 cut(s) 688, 1191, 1375, 1506, 1828
BstFNI CGCG 1 cut(s) 1340
BstHHI GCGC 1 cut(s) 1762
BstKTI GATC 5 cut(s) 534, 1338, 1413, 1431, 1590
BstMAI GTCTC 3 cut(s) 703, 1576, 1719
BstMBI GATC 5 cut(s) 531, 1335, 1410, 1428, 1587
BstMWI GCNNNNNNNGC 6 cut(s) 406, 542, 614, 713, 1757, 1768
BstNSI RCATGY 1 cut(s) 1778
BstSFI CTRYAG 1 cut(s) 177
BstSLI GKGCMC 1 cut(s) 1016
BstUI CGCG 1 cut(s) 1340
BstXI CCANNNNNNTGG 1 cut(s) 718
BsuRI GGCC 5 cut(s) 41, 242, 716, 1464, 1677
BtgI CCRYGG 5 cut(s) 624, 711, 717, 862, 1001
BtsCI GGATG 5 cut(s) 688, 1191, 1375, 1506, 1828
BtsI GCAGTG 1 cut(s) 1452
BtsIMutI CAGTG 2 cut(s) 930, 1452
Cac8I GCNNGC 7 cut(s) 834, 919, 961, 1506, 1671, 1675, 1776
CciI TCATGA 1 cut(s) 1780
CfoI GCGC 1 cut(s) 1762
Cfr10I RCCGGY 1 cut(s) 1265
Cfr13I GGNCC 3 cut(s) 330, 661, 1651
CseI GACGC 1 cut(s) 920
Csp6I GTAC 2 cut(s) 744, 1723
CspAI ACCGGT 1 cut(s) 1265
CspCI CAANNNNNGTGG 4 cut(s) 97, 132, 1407, 1442
CviQI GTAC 2 cut(s) 744, 1723
DdeI CTNAG 6 cut(s) 280, 700, 1101, 1117, 1556, 1679
DpnI GATC 5 cut(s) 533, 1337, 1412, 1430, 1589
DpnII GATC 5 cut(s) 531, 1335, 1410, 1428, 1587
DraIII CACNNNGTG 1 cut(s) 1757
EaeI YGGCCR 2 cut(s) 240, 714
Eam1104I CTCTTC 3 cut(s) 60, 654, 1747
EarI CTCTTC 3 cut(s) 60, 654, 1747
Ecl136II GAGCTC 1 cut(s) 1560
Eco130I CCWWGG 4 cut(s) 624, 711, 717, 862
Eco147I AGGCCT 2 cut(s) 1464, 1677
Eco24I GRGCYC 1 cut(s) 1562
Eco31I GGTCTC 1 cut(s) 1719
Eco47I GGWCC 3 cut(s) 330, 661, 1651
Eco53kI GAGCTC 1 cut(s) 1560
Eco57I CTGAAG 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 1560
EcoRI GAATTC 1 cut(s) 235
EcoT14I CCWWGG 4 cut(s) 624, 711, 717, 862
EcoT22I ATGCAT 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 1562
ErhI CCWWGG 4 cut(s) 624, 711, 717, 862
FalI AAGNNNNNCTT 2 cut(s) 1475, 1507
FaqI GGGAC 1 cut(s) 1205
FokI GGATG 5 cut(s) 695, 1178, 1382, 1513, 1835
FriOI GRGCYC 1 cut(s) 1562
GlaI GCGC 1 cut(s) 1761
GsaI CCCAGC 2 cut(s) 1622, 1771
GsuI CTGGAG 1 cut(s) 1158
HaeIII GGCC 5 cut(s) 41, 242, 716, 1464, 1677
HapII CCGG 2 cut(s) 741, 1266
HgaI GACGC 1 cut(s) 920
HhaI GCGC 1 cut(s) 1762
Hin6I GCGC 1 cut(s) 1760
HinP1I GCGC 1 cut(s) 1760
HindIII AAGCTT 1 cut(s) 1148
HinfI GANTC 5 cut(s) 25, 308, 696, 1274, 1311
HpaII CCGG 2 cut(s) 741, 1266
HphI GGTGA 5 cut(s) 641, 1255, 1405, 1591, 1634
Hpy166II GTNNAC 3 cut(s) 632, 1014, 1452
Hpy188I TCNGA 4 cut(s) 55, 307, 910, 1444
Hpy188III TCNNGA 6 cut(s) 328, 675, 974, 1271, 1426, 1781
Hpy8I GTNNAC 3 cut(s) 632, 1014, 1452
Hpy99I CGWCG 1 cut(s) 641
HpyAV CCTTC 4 cut(s) 105, 1171, 1359, 1489
HpyCH4III ACNGT 5 cut(s) 76, 271, 378, 855, 1018
HpyCH4IV ACGT 3 cut(s) 204, 246, 423
HpyF10VI GCNNNNNNNGC 6 cut(s) 406, 542, 614, 713, 1757, 1768
HpyF3I CTNAG 6 cut(s) 280, 700, 1101, 1117, 1556, 1679
HpySE526I ACGT 3 cut(s) 204, 246, 423
HspAI GCGC 1 cut(s) 1760
KpnI GGTACC 1 cut(s) 747
Kzo9I GATC 5 cut(s) 531, 1335, 1410, 1428, 1587
LmnI GCTCC 2 cut(s) 864, 1110
LweI GCATC 4 cut(s) 207, 1290, 1360, 1491
MaeII ACGT 3 cut(s) 204, 246, 423
MaeIII GTNAC 3 cut(s) 250, 1268, 1622
MalI GATC 5 cut(s) 533, 1337, 1412, 1430, 1589
MboI GATC 5 cut(s) 531, 1335, 1410, 1428, 1587
MboII GAAGA 5 cut(s) 77, 466, 641, 917, 1734
MhlI GDGCHC 2 cut(s) 1016, 1562
MlsI TGGCCA 1 cut(s) 716
MluNI TGGCCA 1 cut(s) 716
MlyI GAGTC 2 cut(s) 705, 1305
MmeI TCCRAC 1 cut(s) 1147
Mox20I TGGCCA 1 cut(s) 716
Mph1103I ATGCAT 1 cut(s) 222
MscI TGGCCA 1 cut(s) 716
MseI TTAA 7 cut(s) 45, 426, 470, 725, 966, 1292, 1514
MslI CAYNNNNRTG 3 cut(s) 415, 716, 1779
Msp20I TGGCCA 1 cut(s) 716
MspA1I CMGCKG 2 cut(s) 1376, 1771
MspI CCGG 2 cut(s) 741, 1266
Mva1269I GAATGC 1 cut(s) 1381
MvnI CGCG 1 cut(s) 1340
MwoI GCNNNNNNNGC 6 cut(s) 406, 542, 614, 713, 1757, 1768
NcoI CCATGG 4 cut(s) 624, 711, 717, 862
NdeII GATC 5 cut(s) 531, 1335, 1410, 1428, 1587
NlaIV GGNNCC 2 cut(s) 662, 745
NmuCI GTSAC 3 cut(s) 250, 1268, 1622
NsiI ATGCAT 1 cut(s) 222
NspI RCATGY 1 cut(s) 1778
NspV TTCGAA 1 cut(s) 1385
PaeI GCATGC 1 cut(s) 1778
PagI TCATGA 1 cut(s) 1780
PceI AGGCCT 2 cut(s) 1464, 1677
PctI GAATGC 1 cut(s) 1381
PfeI GAWTC 3 cut(s) 25, 308, 1274
PflMI CCANNNNNTGG 1 cut(s) 1407
PinAI ACCGGT 1 cut(s) 1265
PleI GAGTC 2 cut(s) 704, 1305
PpsI GAGTC 2 cut(s) 704, 1305
Ppu21I YACGTR 1 cut(s) 205
PshBI ATTAAT 3 cut(s) 470, 725, 1292
PsiI TTATAA 1 cut(s) 1806
Psp124BI GAGCTC 1 cut(s) 1562
Psp1406I AACGTT 1 cut(s) 423
PspFI CCCAGC 2 cut(s) 1618, 1767
PspN4I GGNNCC 2 cut(s) 662, 745
PspPI GGNCC 3 cut(s) 330, 661, 1651
PstI CTGCAG 1 cut(s) 181
PvuII CAGCTG 2 cut(s) 1376, 1771
RsaI GTAC 2 cut(s) 745, 1724
RsaNI GTAC 2 cut(s) 744, 1723
RseI CAYNNNNRTG 3 cut(s) 415, 716, 1779
SacI GAGCTC 1 cut(s) 1562
SaqAI TTAA 7 cut(s) 45, 426, 470, 725, 966, 1292, 1514
Sau3AI GATC 5 cut(s) 531, 1335, 1410, 1428, 1587
Sau96I GGNCC 3 cut(s) 330, 661, 1651
SchI GAGTC 2 cut(s) 705, 1305
SduI GDGCHC 2 cut(s) 1016, 1562
SfaNI GCATC 4 cut(s) 207, 1290, 1360, 1491
SfcI CTRYAG 1 cut(s) 177
SfuI TTCGAA 1 cut(s) 1385
SinI GGWCC 3 cut(s) 330, 661, 1651
SmiMI CAYNNNNRTG 3 cut(s) 415, 716, 1779
SmlI CTYRAG 3 cut(s) 771, 798, 1023
SmoI CTYRAG 3 cut(s) 771, 798, 1023
SphI GCATGC 1 cut(s) 1778
SseBI AGGCCT 2 cut(s) 1464, 1677
SsiI CCGC 4 cut(s) 398, 413, 708, 1340
SstI GAGCTC 1 cut(s) 1562
StuI AGGCCT 2 cut(s) 1464, 1677
StyI CCWWGG 4 cut(s) 624, 711, 717, 862
TaaI ACNGT 5 cut(s) 76, 271, 378, 855, 1018
TaiI ACGT 3 cut(s) 207, 249, 426
TaqI TCGA 4 cut(s) 233, 1255, 1331, 1385
TauI GCSGC 2 cut(s) 710, 1343
TfiI GAWTC 3 cut(s) 25, 308, 1274
Tru1I TTAA 7 cut(s) 45, 426, 470, 725, 966, 1292, 1514
Tru9I TTAA 7 cut(s) 45, 426, 470, 725, 966, 1292, 1514
TscAI CASTG 2 cut(s) 937, 1459
TseFI GTSAC 3 cut(s) 250, 1268, 1622
Tsp45I GTSAC 3 cut(s) 250, 1268, 1622
TspDTI ATGAA 2 cut(s) 358, 1734
TspGWI ACGGA 1 cut(s) 447
TspRI CASTG 2 cut(s) 937, 1459
Van91I CCANNNNNTGG 1 cut(s) 1407
VneI GTGCAC 1 cut(s) 1012
VpaK11BI GGWCC 3 cut(s) 330, 661, 1651
VspI ATTAAT 3 cut(s) 470, 725, 1292
XapI RAATTY 3 cut(s) 235, 969, 1259
XceI RCATGY 1 cut(s) 1778
XcmI CCANNNNNNNNNTGG 1 cut(s) 671
Zsp2I ATGCAT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.