pycom16g00610

ATP-dependent Clp protease adaptor protein ClpS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0016323
Physical Location & Seq
Reverse (-)
372007 .. 373688
1682 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g00610.2

Sequence Viewer

Length: 714 bp
ATGATTAAATTACAATCAAAACCCTGCAATCATTTTTTTGTGAGAATTTGGAAATATTCTCTCCCTGATTTCCTTCTTTCTTCTGAGTTTTTGTGCGTTCTTCTAATCTTACCCACTCATTTGCTCAAGCTACCACCGCCGGAGACTACTCATACTAGACTAGTAGAAATGAGCATGGCTGTTACAGGAACAGCTACGGCCTCGTCGTGTTGCCTGACCAAGATGATCGGATTCGCTCCTCTGTCAGCTCCGCCGCGCACTTGTTGTAAGGTTTTGGCTGTCAAGGAAGGCCCAGAAAGACGGGGAGGTAGTCGCGTGGCTGTGACGGCCAGGTTTGGGTCTTCCAGGGGCGGTGGCGCCGGAGTTTTGGAGCGCCCCAAGTTTGACCAATCCGGATTTGATCCTGCCACCCAACTTCAACAAGGAGGAGATATTGGACGATCAAAGGACAAGAGAGGTGTTGGTAGCGGAGATAGTTACAGAGTTTTGCTGATTGATGATGTTCGCCACACCGAAAAATTAGTTGCAAAGGTGTTGCCCCAAGTTGTTCCATCTGTTACACCTGATGTTGCAAGAGGATTATTTCACAAATCGAGGGAAGATGGTGTCGCGGTTGTTATCGTTACAGTGAAGGAACATGCAGAATTTTACTCCCAGATGATGATTCGTGGCGGGTTACGATCAACAATAGAACCGGATTCAAGTAATCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ClpS

Protein Analysis

238

Amino Acids

25.76

Weight (kDa)

9.43

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016323)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36570
malus_domestica MD16G1006400.v1.1
prunus_persica Prupe.1G344200_v2.0.a1
pyrus_communis pycom16g00610
rosa_chinensis RchiOBHm_Chr4g0446331
rosa_laevigata RLG00000005671
rosa_multiflora Rmu_sc0002266.1_g000003 Rmu_sc0007544.1_g000002
rosa_roxburghii Rroxscaffold_5G00386770
rosa_rugosa Rorug04G0370400
rosa_samantha Rh4AG432000 Rh4BG441300 Rh4CG458600 Rh4DG439900
rosa_wichuraiana Rw4G036880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 356
AccII CGCG 3 cut(s) 256, 315, 611
AccIII TCCGGA 1 cut(s) 392
AciI CCGC 7 cut(s) 137, 251, 254, 351, 468, 611, 672
AclWI GGATC 1 cut(s) 395
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 2 cut(s) 45, 644
AcyI GRCGYC 1 cut(s) 357
AfiI CCNNNNNNNGG 1 cut(s) 336
AgsI TTSAA 2 cut(s) 419, 702
AhlI ACTAGT 1 cut(s) 160
AjnI CCWGG 2 cut(s) 329, 344
AluBI AGCT 3 cut(s) 130, 194, 248
AluI AGCT 3 cut(s) 130, 194, 248
Alw26I GTCTC 1 cut(s) 137
AlwI GGATC 1 cut(s) 395
Aor13HI TCCGGA 1 cut(s) 392
AoxI GGCC 3 cut(s) 198, 289, 327
ApoI RAATTY 2 cut(s) 45, 644
AspLEI GCGC 3 cut(s) 258, 359, 375
AspS9I GGNCC 1 cut(s) 290
BanI GGYRCC 1 cut(s) 356
BbsI GAAGAC 1 cut(s) 333
BccI CCATC 2 cut(s) 559, 596
BceAI ACGGC 2 cut(s) 213, 342
BciT130I CCWGG 2 cut(s) 331, 346
BcoDI GTCTC 1 cut(s) 137
BcuI ACTAGT 1 cut(s) 160
BfaI CTAG 2 cut(s) 156, 161
BfoI RGCGCY 2 cut(s) 360, 376
BisI GCNGC 1 cut(s) 254
BlsI GCNGC 1 cut(s) 255
Bme1390I CCNGG 2 cut(s) 331, 346
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 1 cut(s) 358
BmrFI CCNGG 2 cut(s) 331, 346
BpiI GAAGAC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 345
BsaWI WCCGGW 2 cut(s) 392, 694
Bsc4I CCNNNNNNNGG 1 cut(s) 336
BseAI TCCGGA 1 cut(s) 392
BseBI CCWGG 2 cut(s) 331, 346
BseDI CCNNGG 1 cut(s) 345
BseLI CCNNNNNNNGG 1 cut(s) 336
BseMII CTCAG 1 cut(s) 75
BseRI GAGGAG 2 cut(s) 228, 441
Bsh1236I CGCG 3 cut(s) 256, 315, 611
BshFI GGCC 3 cut(s) 200, 291, 329
BshNI GGYRCC 1 cut(s) 356
BsiSI CCGG 4 cut(s) 140, 360, 393, 695
BslI CCNNNNNNNGG 1 cut(s) 336
BsmAI GTCTC 1 cut(s) 137
BsnI GGCC 3 cut(s) 200, 291, 329
Bsp13I TCCGGA 1 cut(s) 392
Bsp143I GATC 4 cut(s) 225, 400, 440, 680
BspACI CCGC 7 cut(s) 137, 251, 254, 351, 468, 611, 672
BspANI GGCC 3 cut(s) 200, 291, 329
BspCNI CTCAG 1 cut(s) 76
BspEI TCCGGA 1 cut(s) 392
BspFNI CGCG 3 cut(s) 256, 315, 611
BspLI GGNNCC 1 cut(s) 358
BspPI GGATC 1 cut(s) 395
BspT107I GGYRCC 1 cut(s) 356
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 4 cut(s) 225, 400, 440, 680
BssNI GRCGYC 1 cut(s) 357
Bst2UI CCWGG 2 cut(s) 331, 346
Bst4CI ACNGT 1 cut(s) 628
BstACI GRCGYC 1 cut(s) 357
BstDEI CTNAG 1 cut(s) 84
BstFNI CGCG 3 cut(s) 256, 315, 611
BstH2I RGCGCY 2 cut(s) 360, 376
BstHHI GCGC 3 cut(s) 258, 359, 375
BstKTI GATC 4 cut(s) 228, 403, 443, 683
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 4 cut(s) 225, 400, 440, 680
BstMWI GCNNNNNNNGC 2 cut(s) 136, 326
BstNI CCWGG 2 cut(s) 331, 346
BstNSI RCATGY 1 cut(s) 641
BstSCI CCNGG 2 cut(s) 329, 344
BstUI CGCG 3 cut(s) 256, 315, 611
BstV2I GAAGAC 1 cut(s) 333
BsuRI GGCC 3 cut(s) 200, 291, 329
BtsIMutI CAGTG 1 cut(s) 633
CfoI GCGC 3 cut(s) 258, 359, 375
Cfr13I GGNCC 1 cut(s) 290
CviAII CATG 2 cut(s) 175, 638
CviJI RGCY 9 cut(s) 130, 179, 194, 200, 248, 278, 291, 320, 329
CviKI_1 RGCY 9 cut(s) 130, 179, 194, 200, 248, 278, 291, 320, 329
DdeI CTNAG 1 cut(s) 84
DinI GGCGCC 1 cut(s) 358
DpnI GATC 4 cut(s) 227, 402, 442, 682
DpnII GATC 4 cut(s) 225, 400, 440, 680
EaeI YGGCCR 1 cut(s) 327
EciI GGCGGA 1 cut(s) 240
EcoRII CCWGG 2 cut(s) 329, 344
EgeI GGCGCC 1 cut(s) 358
EheI GGCGCC 1 cut(s) 358
FaeI CATG 2 cut(s) 178, 641
FaiI YATR 3 cut(s) 153, 176, 639
FatI CATG 2 cut(s) 174, 637
FauI CCCGC 1 cut(s) 665
Fnu4HI GCNGC 1 cut(s) 254
Fsp4HI GCNGC 1 cut(s) 254
FspBI CTAG 2 cut(s) 156, 161
GlaI GCGC 3 cut(s) 257, 358, 374
GluI GCNGC 1 cut(s) 254
HaeII RGCGCY 2 cut(s) 360, 376
HaeIII GGCC 3 cut(s) 200, 291, 329
HapII CCGG 4 cut(s) 140, 360, 393, 695
HhaI GCGC 3 cut(s) 258, 359, 375
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 2 cut(s) 178, 641
Hin6I GCGC 3 cut(s) 256, 357, 373
HinP1I GCGC 3 cut(s) 256, 357, 373
HinfI GANTC 3 cut(s) 231, 664, 698
HpaII CCGG 4 cut(s) 140, 360, 393, 695
Hpy188I TCNGA 2 cut(s) 85, 230
Hpy188III TCNNGA 1 cut(s) 393
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 3 cut(s) 83, 281, 625
HpyCH4III ACNGT 1 cut(s) 628
HpyCH4V TGCA 4 cut(s) 27, 527, 572, 641
HpyF10VI GCNNNNNNNGC 2 cut(s) 136, 326
HpyF3I CTNAG 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 2 cut(s) 178, 641
HspAI GCGC 3 cut(s) 256, 357, 373
KasI GGCGCC 1 cut(s) 356
Kpn2I TCCGGA 1 cut(s) 392
Kzo9I GATC 4 cut(s) 225, 400, 440, 680
LmnI GCTCC 3 cut(s) 241, 253, 370
MaeI CTAG 2 cut(s) 156, 161
MaeIII GTNAC 6 cut(s) 181, 322, 476, 556, 622, 675
MalI GATC 4 cut(s) 227, 402, 442, 682
MboI GATC 4 cut(s) 225, 400, 440, 680
MboII GAAGA 4 cut(s) 72, 92, 333, 611
MluCI AATT 4 cut(s) 8, 45, 518, 644
Mly113I GGCGCC 1 cut(s) 357
MnlI CCTC 7 cut(s) 211, 249, 299, 419, 449, 569, 588
MroI TCCGGA 1 cut(s) 392
MseI TTAA 1 cut(s) 6
MspI CCGG 4 cut(s) 140, 360, 393, 695
MspR9I CCNGG 2 cut(s) 331, 346
MvaI CCWGG 2 cut(s) 331, 346
MvnI CGCG 3 cut(s) 256, 315, 611
MwoI GCNNNNNNNGC 2 cut(s) 136, 326
NarI GGCGCC 1 cut(s) 357
NdeII GATC 4 cut(s) 225, 400, 440, 680
NlaIII CATG 2 cut(s) 178, 641
NlaIV GGNNCC 1 cut(s) 358
NmuCI GTSAC 1 cut(s) 322
NspI RCATGY 1 cut(s) 641
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PfeI GAWTC 3 cut(s) 231, 664, 698
PkrI GCNGC 1 cut(s) 255
PluTI GGCGCC 1 cut(s) 360
Psp6I CCWGG 2 cut(s) 329, 344
PspGI CCWGG 2 cut(s) 329, 344
PspN4I GGNNCC 1 cut(s) 358
PspPI GGNCC 1 cut(s) 290
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 1 cut(s) 254
Sau3AI GATC 4 cut(s) 225, 400, 440, 680
Sau96I GGNCC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 331, 346
SetI ASST 9 cut(s) 132, 196, 250, 273, 310, 335, 460, 534, 565
SfoI GGCGCC 1 cut(s) 358
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SpeI ACTAGT 1 cut(s) 160
Sse9I AATT 4 cut(s) 8, 45, 518, 644
SsiI CCGC 7 cut(s) 137, 251, 254, 351, 468, 611, 672
SspDI GGCGCC 1 cut(s) 356
SspI AATATT 1 cut(s) 56
SspMI CTAG 2 cut(s) 156, 161
StyD4I CCNGG 2 cut(s) 329, 344
TaaI ACNGT 1 cut(s) 628
TaqI TCGA 1 cut(s) 593
TasI AATT 4 cut(s) 8, 45, 518, 644
TauI GCSGC 1 cut(s) 256
TfiI GAWTC 3 cut(s) 231, 664, 698
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TscAI CASTG 1 cut(s) 633
TseFI GTSAC 1 cut(s) 322
Tsp45I GTSAC 1 cut(s) 322
TspRI CASTG 1 cut(s) 633
XapI RAATTY 2 cut(s) 45, 644
XceI RCATGY 1 cut(s) 641
XspI CTAG 2 cut(s) 156, 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.