pycom16g05950

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010211
Physical Location & Seq
Reverse (-)
3787210 .. 3787956
747 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 702 bp
ATGCAAACACCTCCATCTTCGAACGTTATGATATCTTGGTGGTTTCCTTGGATATCAAGTAACGGATTCTTTTGTCAGATTAACCACAGTTCAAGCATAACCACAGAAGAACATGCATATGAGGTCAAAGGTGCAGCAGGTCTTTTCTCTTGGGATTATCTGTTTTCGCTACGGAGCCCCGCTACTGTCAAAGCTGGTGAGCAGCCTGTGCTCTGCAGCAGGCATCTCATGCTTCTCATATCTGGACAGGTTTTCATCCAATATGATATAAACAAGAGCAATGCTGAGTTTGCTTTGGATTACGGATTCCTGGATTCCAACGCTAATCGCAATGGATTTACCTTAACGCTGGAAATATCCAAATCAGACCCATTTTACGAGGACAAGCTGGACATTGCCGAGACAAATGGTTTGGGTGCAACTGCGTACTTCGACATATTTTTGGAGAGTTCGCTTCCACCTGCATTGATTCCTTATCTGCGGCTGGTAGCTCTTGGAGGAACCGACGCTTTCCTCTTGGAATCTATATTCAGGAACGATATCTGGGGCCACCTTCAACTGCCGGTGAGCCCTGCCAACGAAGAGCTCATATGCAGAGTAGTCCAAGACGCCTGCAAATCCGCTCTTTCCGGCTATCCCACCACTATCGAAGAGGTATGTCTTCATTCCTTAATACATCTGCATGCTTTAGTACATTTGTAA

Protein Analysis

234

Amino Acids

25.94

Weight (kDa)

4.85

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rubis-subs-bind PF09273 120 - 218 3e-25 Rubisco LSMT substrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010211)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14030
fragaria_vesca FvH4_4g30820 FvH4_4g30820 FvH4_4g30820
malus_domestica MD16G1067900.v1.1
prunus_persica Prupe.1G278900_v2.0.a1
pyrus_communis pycom13g06090 pycom16g05950
rosa_chinensis RchiOBHm_Chr4g0439211
rosa_laevigata RLG00000006287
rosa_multiflora Rmu_sc0002236.1_g000013 Rmu_sc0006598.1_g000019
rosa_roxburghii Rroxscaffold_5G00380200
rosa_rugosa Rorug04G0317100
rosa_samantha Rh4AG367600 Rh4BG379200 Rh4CG393600 Rh4DG373800
rosa_wichuraiana Rw0G005650 Rw0G011640 Rw5G028540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 469
Acc36I ACCTGC 2 cut(s) 128, 469
AccBSI CCGCTC 1 cut(s) 623
AciI CCGC 3 cut(s) 180, 481, 621
AclI AACGTT 1 cut(s) 24
AcyI GRCGYC 1 cut(s) 609
AfaI GTAC 2 cut(s) 428, 693
AgsI TTSAA 2 cut(s) 93, 557
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 4 cut(s) 194, 388, 491, 586
AluI AGCT 4 cut(s) 194, 388, 491, 586
Alw21I GWGCWC 2 cut(s) 213, 588
Alw26I GTCTC 1 cut(s) 395
AoxI GGCC 1 cut(s) 547
ApeKI GCWGC 3 cut(s) 134, 202, 216
ArsI GACNNNNNNTTYG 2 cut(s) 394, 426
AspS9I GGNCC 1 cut(s) 547
AsuHPI GGTGA 2 cut(s) 209, 577
AsuII TTCGAA 1 cut(s) 20
BanII GRGCYC 3 cut(s) 179, 572, 588
BbsI GAAGAC 1 cut(s) 653
Bbv12I GWGCWC 2 cut(s) 213, 588
BbvI GCAGC 3 cut(s) 146, 214, 228
BccI CCATC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 311
BcoDI GTCTC 1 cut(s) 395
BfmI CTRYAG 1 cut(s) 214
BfuAI ACCTGC 2 cut(s) 128, 469
BisI GCNGC 4 cut(s) 135, 203, 217, 482
BlsI GCNGC 4 cut(s) 136, 204, 218, 483
Bme1390I CCNGG 1 cut(s) 311
BmgT120I GGNCC 1 cut(s) 547
BmiI GGNNCC 3 cut(s) 176, 502, 548
BmrFI CCNGG 1 cut(s) 311
BmsI GCATC 1 cut(s) 232
BpiI GAAGAC 1 cut(s) 653
Bpu14I TTCGAA 1 cut(s) 20
BsaHI GRCGYC 1 cut(s) 609
BsaJI CCNNGG 1 cut(s) 47
Bse118I RCCGGY 1 cut(s) 562
Bse3DI GCAATG 3 cut(s) 286, 337, 393
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 1 cut(s) 47
BseGI GGATG 1 cut(s) 255
BseMI GCAATG 3 cut(s) 286, 337, 393
BseMII CTCAG 1 cut(s) 276
BseXI GCAGC 3 cut(s) 146, 214, 228
BsgI GTGCAG 1 cut(s) 153
BshFI GGCC 1 cut(s) 549
BsiHKAI GWGCWC 2 cut(s) 213, 588
BsiSI CCGG 2 cut(s) 563, 630
BsmAI GTCTC 1 cut(s) 395
BsnI GGCC 1 cut(s) 549
Bsp119I TTCGAA 1 cut(s) 20
Bsp1286I GDGCHC 4 cut(s) 179, 213, 572, 588
BspACI CCGC 3 cut(s) 180, 481, 621
BspANI GGCC 1 cut(s) 549
BspCNI CTCAG 1 cut(s) 277
BspLI GGNNCC 3 cut(s) 176, 502, 548
BspMAI CTGCAG 1 cut(s) 218
BspMI ACCTGC 2 cut(s) 128, 469
BspQI GCTCTTC 1 cut(s) 576
BspT104I TTCGAA 1 cut(s) 20
BsrBI CCGCTC 1 cut(s) 623
BsrDI GCAATG 3 cut(s) 286, 337, 393
BsrFI RCCGGY 1 cut(s) 562
BssAI RCCGGY 1 cut(s) 562
BssECI CCNNGG 1 cut(s) 47
BssNI GRCGYC 1 cut(s) 609
BssT1I CCWWGG 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 2 cut(s) 89, 187
Bst6I CTCTTC 2 cut(s) 576, 645
BstACI GRCGYC 1 cut(s) 609
BstAPI GCANNNNNTGC 2 cut(s) 208, 229
BstBI TTCGAA 1 cut(s) 20
BstC8I GCNNGC 3 cut(s) 221, 613, 684
BstDEI CTNAG 1 cut(s) 285
BstF5I GGATG 1 cut(s) 255
BstMAI GTCTC 1 cut(s) 395
BstMWI GCNNNNNNNGC 3 cut(s) 208, 229, 290
BstNI CCWGG 1 cut(s) 311
BstNSI RCATGY 2 cut(s) 116, 686
BstSCI CCNGG 1 cut(s) 309
BstSFI CTRYAG 1 cut(s) 214
BstV1I GCAGC 3 cut(s) 146, 214, 228
BstV2I GAAGAC 1 cut(s) 653
BsuRI GGCC 1 cut(s) 549
BtsCI GGATG 1 cut(s) 255
BveI ACCTGC 2 cut(s) 128, 469
Cac8I GCNNGC 3 cut(s) 221, 613, 684
Cfr10I RCCGGY 1 cut(s) 562
Cfr13I GGNCC 1 cut(s) 547
CseI GACGC 2 cut(s) 515, 617
Csp6I GTAC 2 cut(s) 427, 692
CspCI CAANNNNNGTGG 2 cut(s) 447, 482
CviAII CATG 3 cut(s) 113, 229, 683
CviQI GTAC 2 cut(s) 427, 692
DdeI CTNAG 1 cut(s) 285
Eam1104I CTCTTC 2 cut(s) 576, 645
EarI CTCTTC 2 cut(s) 576, 645
Ecl136II GAGCTC 1 cut(s) 586
Eco130I CCWWGG 1 cut(s) 47
Eco24I GRGCYC 3 cut(s) 179, 572, 588
Eco32I GATATC 3 cut(s) 33, 54, 541
Eco53kI GAGCTC 1 cut(s) 586
EcoICRI GAGCTC 1 cut(s) 586
EcoRII CCWGG 1 cut(s) 309
EcoRV GATATC 3 cut(s) 33, 54, 541
EcoT14I CCWWGG 1 cut(s) 47
EcoT22I ATGCAT 1 cut(s) 118
EcoT38I GRGCYC 3 cut(s) 179, 572, 588
ErhI CCWWGG 1 cut(s) 47
FaeI CATG 3 cut(s) 116, 232, 686
FatI CATG 3 cut(s) 112, 228, 682
FauI CCCGC 1 cut(s) 187
FauNDI CATATG 2 cut(s) 118, 590
Fnu4HI GCNGC 4 cut(s) 135, 203, 217, 482
FokI GGATG 1 cut(s) 242
FriOI GRGCYC 3 cut(s) 179, 572, 588
Fsp4HI GCNGC 4 cut(s) 135, 203, 217, 482
GluI GCNGC 4 cut(s) 135, 203, 217, 482
HaeIII GGCC 1 cut(s) 549
HapII CCGG 2 cut(s) 563, 630
HgaI GACGC 2 cut(s) 515, 617
Hin1I GRCGYC 1 cut(s) 609
Hin1II CATG 3 cut(s) 116, 232, 686
HinfI GANTC 5 cut(s) 66, 306, 314, 469, 521
HpaII CCGG 2 cut(s) 563, 630
HphI GGTGA 2 cut(s) 209, 577
Hpy188I TCNGA 2 cut(s) 78, 367
Hpy188III TCNNGA 2 cut(s) 243, 532
Hpy99I CGWCG 1 cut(s) 509
HpyAV CCTTC 1 cut(s) 563
HpyCH4III ACNGT 2 cut(s) 89, 187
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 9 cut(s) 4, 116, 134, 216, 419, 464, 594, 615, 682
HpyF10VI GCNNNNNNNGC 3 cut(s) 208, 229, 290
HpyF3I CTNAG 1 cut(s) 285
HpySE526I ACGT 1 cut(s) 24
Hsp92I GRCGYC 1 cut(s) 609
Hsp92II CATG 3 cut(s) 116, 232, 686
LguI GCTCTTC 1 cut(s) 576
LmnI GCTCC 1 cut(s) 174
Lsp1109I GCAGC 3 cut(s) 146, 214, 228
LweI GCATC 1 cut(s) 232
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 1 cut(s) 59
MbiI CCGCTC 1 cut(s) 623
MboII GAAGA 5 cut(s) 9, 119, 593, 653, 662
MhlI GDGCHC 4 cut(s) 179, 213, 572, 588
MmeI TCCRAC 1 cut(s) 342
MnlI CCTC 6 cut(s) 21, 115, 373, 491, 524, 646
Mph1103I ATGCAT 1 cut(s) 118
MseI TTAA 3 cut(s) 81, 344, 671
MslI CAYNNNNRTG 2 cut(s) 117, 681
MspI CCGG 2 cut(s) 563, 630
MspR9I CCNGG 1 cut(s) 311
MvaI CCWGG 1 cut(s) 311
MwoI GCNNNNNNNGC 3 cut(s) 208, 229, 290
NdeI CATATG 2 cut(s) 118, 590
NlaIII CATG 3 cut(s) 116, 232, 686
NlaIV GGNNCC 3 cut(s) 176, 502, 548
NmeAIII GCCGAG 1 cut(s) 424
NsiI ATGCAT 1 cut(s) 118
NspI RCATGY 2 cut(s) 116, 686
NspV TTCGAA 1 cut(s) 20
PaeI GCATGC 1 cut(s) 686
PaqCI CACCTGC 1 cut(s) 469
PciSI GCTCTTC 1 cut(s) 576
PfeI GAWTC 5 cut(s) 66, 306, 314, 469, 521
PfoI TCCNGGA 1 cut(s) 309
PkrI GCNGC 4 cut(s) 136, 204, 218, 483
Psp124BI GAGCTC 1 cut(s) 588
Psp1406I AACGTT 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 3 cut(s) 176, 502, 548
PspPI GGNCC 1 cut(s) 547
PstI CTGCAG 1 cut(s) 218
RsaI GTAC 2 cut(s) 428, 693
RsaNI GTAC 2 cut(s) 427, 692
RseI CAYNNNNRTG 2 cut(s) 117, 681
SacI GAGCTC 1 cut(s) 588
SapI GCTCTTC 1 cut(s) 576
SaqAI TTAA 3 cut(s) 81, 344, 671
SatI GCNGC 4 cut(s) 135, 203, 217, 482
Sau96I GGNCC 1 cut(s) 547
ScrFI CCNGG 1 cut(s) 311
SduI GDGCHC 4 cut(s) 179, 213, 572, 588
SfaNI GCATC 1 cut(s) 232
SfcI CTRYAG 1 cut(s) 214
SfuI TTCGAA 1 cut(s) 20
SmiMI CAYNNNNRTG 2 cut(s) 117, 681
SphI GCATGC 1 cut(s) 686
SsiI CCGC 3 cut(s) 180, 481, 621
SstI GAGCTC 1 cut(s) 588
StyD4I CCNGG 1 cut(s) 309
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 2 cut(s) 89, 187
TaiI ACGT 1 cut(s) 27
TaqI TCGA 3 cut(s) 20, 432, 648
TatI WGTACW 1 cut(s) 691
TauI GCSGC 1 cut(s) 484
TfiI GAWTC 5 cut(s) 66, 306, 314, 469, 521
Tru1I TTAA 3 cut(s) 81, 344, 671
Tru9I TTAA 3 cut(s) 81, 344, 671
TseI GCWGC 3 cut(s) 134, 202, 216
TspDTI ATGAA 2 cut(s) 244, 653
TspGWI ACGGA 3 cut(s) 78, 187, 318
XceI RCATGY 2 cut(s) 116, 686
Zsp2I ATGCAT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.