pycom16g07400

IAA-amino acid hydrolase ILR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0015613
Physical Location & Seq
Forward (+)
4852612 .. 4853810
1199 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 783 bp
ATGGACTTTCTTCTTCTGCAACTTTTGTTCCTAATAATCTCCCTTGCCATGCCCTTGTGCTTTTCTCTGAAACTCCAAATCAACTTATCCACCACATATCAGACTTATTCAGTGAAACACCAAATAGTTAGGCTGGCCAACGAACCCAGCACAGTGAATTGGATGAAAAACGTGAGGAGGGAAATGCATGAAAACCCAGAACTTTCATATGAAGAAATCGAAACAAGTGCACTCATTCGCCGCGAGCTTGATAAACTCGGTGTTGCTTATAAATGGCCTGTGGCTAAAACTGGTGTTGTAGCCACGATTGGCTCTGGCTCTCCTCCATTTGTTGCTCTAAGAGCTGATATGGATGCTTTGCCTATTCAGGAAATGGTGGACTGGGAGAACAAGAGCAAAGTGGAGGGAAAAATGCATGCTTGCGGCCACGACGGCCACGTCGCCATGCTTCTTGGCGCTGCAAAAGTACTAAAACAACTGCAAGACACATTACAGGGAACCGTTGTACTTATATTTCAACCGGCTGAGGAACGTGGTTCAGGCGCAAAGGACATGATTGAAGAAGGGGTGCTTGATAATGTGGAGGCAGTTTTTGGGATGCACGTAGCGCCTGTATATCCGACTGGCGTTGTTGCATTGAGGCCTGGAGAATTTCTAGCCGGTTGTGGGAGCTTCAAAGCGAAAATTCACGGGAAAGGGGGTCATGCAGCAATTCCTCAGCAATCCATTGATCCGATTTTGGCAGCATCAACATCTGTAATTAGTTTGCAGAACATCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

261

Amino Acids

28.34

Weight (kDa)

6.07

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 112 - 230 2.5e-32 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015613)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35100
malus_domestica MD13G1086300.v1.1 MD16G1085700.v1.1
prunus_persica Prupe.1G260400_v2.0.a1
pyrus_communis pycom13g07450 pycom16g07400
rosa_chinensis RchiOBHm_Chr4g0444701
rosa_laevigata RLG00000005812
rosa_multiflora Rmu_sc0012443.1_g000011
rosa_roxburghii Rroxscaffold_5G00385200
rosa_rugosa Rorug04G0354600
rosa_samantha Rh4AG414700 Rh4BG426400 Rh4CG441300 Rh4DG422300
rosa_wichuraiana Rw4G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 270
AasI GACNNNNNNGTC 1 cut(s) 437
AccII CGCG 1 cut(s) 243
AciI CCGC 2 cut(s) 241, 423
AclWI GGATC 1 cut(s) 725
AcoI YGGCCR 3 cut(s) 135, 424, 433
AcsI RAATTY 2 cut(s) 650, 684
AfaI GTAC 2 cut(s) 468, 507
AfiI CCNNNNNNNGG 1 cut(s) 666
AgsI TTSAA 3 cut(s) 518, 560, 676
AjiI CACGTC 1 cut(s) 439
AjnI CCWGG 1 cut(s) 643
AluBI AGCT 3 cut(s) 247, 344, 672
AluI AGCT 3 cut(s) 247, 344, 672
Alw21I GWGCWC 1 cut(s) 232
Alw44I GTGCAC 1 cut(s) 228
AlwI GGATC 1 cut(s) 725
AoxI GGCC 5 cut(s) 135, 275, 424, 433, 641
ApaLI GTGCAC 1 cut(s) 228
ApeKI GCWGC 3 cut(s) 458, 707, 743
ApoI RAATTY 2 cut(s) 650, 684
AspLEI GCGC 3 cut(s) 458, 545, 610
BaeGI GKGCMC 1 cut(s) 232
BalI TGGCCA 1 cut(s) 137
Bbv12I GWGCWC 1 cut(s) 232
BbvCI CCTCAGC 2 cut(s) 525, 717
BbvI GCAGC 3 cut(s) 445, 719, 755
BceAI ACGGC 1 cut(s) 448
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciT130I CCWGG 1 cut(s) 645
BfaI CTAG 2 cut(s) 656, 781
BfoI RGCGCY 2 cut(s) 459, 611
BglI GCCNNNNNGGC 1 cut(s) 432
BisI GCNGC 5 cut(s) 241, 424, 459, 708, 744
BlsI GCNGC 5 cut(s) 242, 425, 460, 709, 745
BmcAI AGTACT 1 cut(s) 468
Bme1390I CCNGG 1 cut(s) 645
BmgBI CACGTC 1 cut(s) 439
BmiI GGNNCC 1 cut(s) 499
BmrFI CCNGG 1 cut(s) 645
BmrI ACTGGG 1 cut(s) 391
BmsI GCATC 3 cut(s) 343, 588, 755
BmuI ACTGGG 1 cut(s) 391
BpmI CTGGAG 1 cut(s) 666
Bpu10I CCTNAGC 2 cut(s) 525, 717
BsaAI YACGTR 1 cut(s) 604
Bsc4I CCNNNNNNNGG 1 cut(s) 666
Bse118I RCCGGY 2 cut(s) 520, 659
Bse1I ACTGG 3 cut(s) 295, 386, 628
BseBI CCWGG 1 cut(s) 645
BseGI GGATG 3 cut(s) 168, 358, 603
BseLI CCNNNNNNNGG 1 cut(s) 666
BseMII CTCAG 2 cut(s) 516, 731
BseNI ACTGG 3 cut(s) 295, 386, 628
BseRI GAGGAG 2 cut(s) 190, 312
BseSI GKGCMC 1 cut(s) 232
BseXI GCAGC 3 cut(s) 445, 719, 755
BseYI CCCAGC 1 cut(s) 146
Bsh1236I CGCG 1 cut(s) 243
BshFI GGCC 5 cut(s) 137, 277, 426, 435, 643
BsiHKAI GWGCWC 1 cut(s) 232
BsiSI CCGG 2 cut(s) 521, 660
BslI CCNNNNNNNGG 1 cut(s) 666
BsnI GGCC 5 cut(s) 137, 277, 426, 435, 643
Bsp1286I GDGCHC 1 cut(s) 232
Bsp143I GATC 1 cut(s) 730
BspACI CCGC 2 cut(s) 241, 423
BspANI GGCC 5 cut(s) 137, 277, 426, 435, 643
BspCNI CTCAG 2 cut(s) 517, 730
BspFNI CGCG 1 cut(s) 243
BspLI GGNNCC 1 cut(s) 499
BspPI GGATC 1 cut(s) 725
BsrFI RCCGGY 2 cut(s) 520, 659
BsrI ACTGG 3 cut(s) 295, 386, 628
BssAI RCCGGY 2 cut(s) 520, 659
BssMI GATC 1 cut(s) 730
Bst2UI CCWGG 1 cut(s) 645
Bst4CI ACNGT 2 cut(s) 154, 502
BstBAI YACGTR 1 cut(s) 604
BstC8I GCNNGC 4 cut(s) 135, 245, 417, 421
BstDEI CTNAG 3 cut(s) 338, 525, 717
BstF5I GGATG 3 cut(s) 168, 358, 603
BstFNI CGCG 1 cut(s) 243
BstH2I RGCGCY 2 cut(s) 459, 611
BstHHI GCGC 3 cut(s) 458, 545, 610
BstKTI GATC 1 cut(s) 733
BstMBI GATC 1 cut(s) 730
BstMWI GCNNNNNNNGC 3 cut(s) 341, 432, 607
BstNI CCWGG 1 cut(s) 645
BstNSI RCATGY 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 643
BstSLI GKGCMC 1 cut(s) 232
BstUI CGCG 1 cut(s) 243
BstV1I GCAGC 3 cut(s) 445, 719, 755
BsuRI GGCC 5 cut(s) 137, 277, 426, 435, 643
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 3 cut(s) 168, 358, 603
BtsIMutI CAGTG 2 cut(s) 117, 159
Cac8I GCNNGC 4 cut(s) 135, 245, 417, 421
CfoI GCGC 3 cut(s) 458, 545, 610
Cfr10I RCCGGY 2 cut(s) 520, 659
Csp6I GTAC 2 cut(s) 467, 506
CviAII CATG 6 cut(s) 49, 188, 416, 445, 553, 704
CviQI GTAC 2 cut(s) 467, 506
DdeI CTNAG 3 cut(s) 338, 525, 717
DpnI GATC 1 cut(s) 732
DpnII GATC 1 cut(s) 730
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
EaeI YGGCCR 3 cut(s) 135, 424, 433
Eco147I AGGCCT 1 cut(s) 643
EcoRII CCWGG 1 cut(s) 643
EcoT22I ATGCAT 2 cut(s) 189, 417
FaeI CATG 6 cut(s) 52, 191, 419, 448, 556, 707
FalI AAGNNNNNCTT 2 cut(s) 555, 587
FatI CATG 6 cut(s) 48, 187, 415, 444, 552, 703
FauNDI CATATG 1 cut(s) 208
Fnu4HI GCNGC 5 cut(s) 241, 424, 459, 708, 744
FokI GGATG 3 cut(s) 175, 365, 610
Fsp4HI GCNGC 5 cut(s) 241, 424, 459, 708, 744
FspBI CTAG 2 cut(s) 656, 781
GlaI GCGC 3 cut(s) 457, 544, 609
GluI GCNGC 5 cut(s) 241, 424, 459, 708, 744
GsaI CCCAGC 1 cut(s) 150
GsuI CTGGAG 1 cut(s) 666
HaeII RGCGCY 2 cut(s) 459, 611
HaeIII GGCC 5 cut(s) 137, 277, 426, 435, 643
HapII CCGG 2 cut(s) 521, 660
HhaI GCGC 3 cut(s) 458, 545, 610
Hin1II CATG 6 cut(s) 52, 191, 419, 448, 556, 707
Hin6I GCGC 3 cut(s) 456, 543, 608
HinP1I GCGC 3 cut(s) 456, 543, 608
HpaII CCGG 2 cut(s) 521, 660
Hpy166II GTNNAC 2 cut(s) 230, 379
Hpy188I TCNGA 4 cut(s) 69, 102, 621, 735
Hpy188III TCNNGA 1 cut(s) 368
Hpy8I GTNNAC 2 cut(s) 230, 379
Hpy99I CGWCG 2 cut(s) 434, 443
HpyAV CCTTC 1 cut(s) 557
HpyCH4III ACNGT 2 cut(s) 154, 502
HpyCH4IV ACGT 4 cut(s) 171, 438, 532, 603
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 432, 607
HpyF3I CTNAG 3 cut(s) 338, 525, 717
HpySE526I ACGT 4 cut(s) 171, 438, 532, 603
Hsp92II CATG 6 cut(s) 52, 191, 419, 448, 556, 707
HspAI GCGC 3 cut(s) 456, 543, 608
Kzo9I GATC 1 cut(s) 730
LmnI GCTCC 1 cut(s) 669
Lsp1109I GCAGC 3 cut(s) 445, 719, 755
LweI GCATC 3 cut(s) 343, 588, 755
MaeI CTAG 2 cut(s) 656, 781
MaeII ACGT 4 cut(s) 171, 438, 532, 603
MalI GATC 1 cut(s) 732
MboI GATC 1 cut(s) 730
MboII GAAGA 3 cut(s) 5, 224, 572
MhlI GDGCHC 1 cut(s) 232
MlsI TGGCCA 1 cut(s) 137
MluCI AATT 5 cut(s) 157, 650, 684, 711, 759
MluNI TGGCCA 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 644
MnlI CCTC 8 cut(s) 168, 171, 333, 397, 520, 577, 633, 726
Mox20I TGGCCA 1 cut(s) 137
Mph1103I ATGCAT 2 cut(s) 189, 417
MscI TGGCCA 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 137
MspI CCGG 2 cut(s) 521, 660
MspR9I CCNGG 1 cut(s) 645
MvaI CCWGG 1 cut(s) 645
MvnI CGCG 1 cut(s) 243
MwoI GCNNNNNNNGC 3 cut(s) 341, 432, 607
NdeI CATATG 1 cut(s) 208
NdeII GATC 1 cut(s) 730
NlaIII CATG 6 cut(s) 52, 191, 419, 448, 556, 707
NlaIV GGNNCC 1 cut(s) 499
NsiI ATGCAT 2 cut(s) 189, 417
NspI RCATGY 1 cut(s) 419
PaeI GCATGC 1 cut(s) 419
PceI AGGCCT 1 cut(s) 643
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PkrI GCNGC 5 cut(s) 242, 425, 460, 709, 745
Ppu21I YACGTR 1 cut(s) 604
PsiI TTATAA 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 643
PspFI CCCAGC 1 cut(s) 146
PspGI CCWGG 1 cut(s) 643
PspN4I GGNNCC 1 cut(s) 499
RsaI GTAC 2 cut(s) 468, 507
RsaNI GTAC 2 cut(s) 467, 506
SatI GCNGC 5 cut(s) 241, 424, 459, 708, 744
Sau3AI GATC 1 cut(s) 730
ScaI AGTACT 1 cut(s) 468
ScrFI CCNGG 1 cut(s) 645
SduI GDGCHC 1 cut(s) 232
SetI ASST 7 cut(s) 174, 249, 346, 441, 535, 606, 674
SfaNI GCATC 3 cut(s) 343, 588, 755
SfiI GGCCNNNNNGGCC 1 cut(s) 432
SphI GCATGC 1 cut(s) 419
Sse9I AATT 5 cut(s) 157, 650, 684, 711, 759
SseBI AGGCCT 1 cut(s) 643
SsiI CCGC 2 cut(s) 241, 423
SspMI CTAG 2 cut(s) 656, 781
StuI AGGCCT 1 cut(s) 643
StyD4I CCNGG 1 cut(s) 643
TaaI ACNGT 2 cut(s) 154, 502
TaiI ACGT 4 cut(s) 174, 441, 535, 606
TaqI TCGA 1 cut(s) 219
TasI AATT 5 cut(s) 157, 650, 684, 711, 759
TatI WGTACW 2 cut(s) 466, 505
TauI GCSGC 2 cut(s) 243, 426
TscAI CASTG 2 cut(s) 117, 159
TseI GCWGC 3 cut(s) 458, 707, 743
TspDTI ATGAA 4 cut(s) 179, 195, 204, 225
TspRI CASTG 2 cut(s) 117, 159
VneI GTGCAC 1 cut(s) 228
XapI RAATTY 2 cut(s) 650, 684
XceI RCATGY 1 cut(s) 419
XspI CTAG 2 cut(s) 656, 781
ZrmI AGTACT 1 cut(s) 468
Zsp2I ATGCAT 2 cut(s) 189, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.