pycom16g09210

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014515
Physical Location & Seq
Reverse (-)
6170617 .. 6175778
5162 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2808 bp
ATGGGTTTCTCAGTCGTCCTGTTCATAATTTATCTTTGCTTTATATCATCTGTTATTTCCCAGGTTCAAGATTTTGTGAGCATAGATTGTGGAGGGACAAGTAACCACGCTGACACAAGCACTGGACTGGCATGGATTTCAGACATGCGATTCATGTCCCAGGGAAAATCAGTCCAGGTCGAAAACCCTAATGGAAACTTGCTGCAGTTTCAGAGACGCAGGGATTTTCCCATAGACAGCAAGAAGTACTGCTATACGTTGAACACTGTGGAGAGGAGGAGGTATTTAGTCCGGGCAACATTTCAGTACGGCAGTCTCAAAAGTGAAGACACATATCCGAAATTCGAGCTTTACTTGGATGCCACGCAGTGGGCAACCGTGACTATTTTTGATGCTTCAAGAACATATGTGAATGAGATGATCATCAGGGCTTCGTCGGAATCTGTCGATGTGTGTATATGTTGTGCAACAACTGGGTTTCCGTTTATATCGACTTTGGAGCTGAGGCCTTTGAATCTTTCGATGTATGCCACGGACTATGAGGATGAGTTTTTCTTGAAAGTTGCAGCTAGGGTGAATTTTGGTGCTCCAAGCAAAGATGCTATAAGGTACCCGGATGATCCATATGACAGAATTTGGGATTCTGATCTTGTGAAAAGGCAGAATTATCTGGTTGGAGTGGCACCAGGTACAGAAAGAATCAGTACATCAAGGGATGTAGACACAAGGAGTAGAGAGTACCCCCCTGTTAAAGTAATGCAGACCGCGGTAGTTGGCACAAAGGGAGTGCTGAGCTACAGATTAAATCTGGATGGCTTCCCAGGAATGGGTCGAGCCTATGCTTACTTTGCGGAAATTGAGGATTTAGGCGCAAATGAGATCCGGAAATTCAAGTTGGAGCAACCTTTCTCGGCAGACTATAATAGTGCAGAGGTAAATATTGCTGAGAATGCAAATGGGGACTACACGCTTTATGAGCCGAGCTACATGAATGTGAGTCTGGAGTATGTTCTGCCATTTTCCTTTGTTAAGACCCCTGATTCTACGCGAGGGCCGCTTCTAAATGCAATGGAGATAAGTAAATATGTTCCAATCGCTGCAAAGACCGATAGGCGAGATTTAGTGGCGGTTCTGAATGTGCTTCAGTTCATGTCTGCTGAAAGTCTATCAGTAGATGAAGGTGATCCTTGCGCTCCAACTCCGTGGGAATGGGTGAATTGTAGCTCCACAGCACCACCAAGAATCACAAAAATTGACCTTTCAGGAAAGAATGTGAAGGGTGAAATCCCCGTGGAGCTTAAAAATCTGCAGGAGTTGACAGAGTTGTGGTTGGGTGGTAACTACCTTACCGGACAATTTCCTGACATCAGTAATCTTATCAATTTAAAGATTTTGCATCTAGAGAACAACAAATTGAGTGGTTCACTACCTTCTTACCTGGCTAGCTTGCCAAGCTTACAAGAACTGTACATACAGAACAACTCTTTCAGTGGGGAAATTCCTGCAGGACTGTTAAGGGGGAAAATCACATTTAAATATGAATATAATCTTGGATTACGTAAGGGGGAACGAAAACAGAAGCATTTCAAGTTGATAATTGGAATTTCTGTTGGAGTAGTTGCAACTGTATCATTGTTATTATTAGGGACTCTATTGATATTGCGTAATCTTCAAAGAAAGTCATCTTTTCAGAGAAGTAATGGAAAAGGTGACTCGCTGCGTGCCCGCACCAAGCCTTCAACTGGCTATTCAATTTCTCGTATGGATGAAGGCGTAGCTTGCTACATTACTCTCCCTGATCTGGAAGAGGCAACTAGCAGCTTTTCGAAAAAAATTGGCAAAGGAAGCTTTGGGTCTGTATATTATGGAAAGATGAAAGATGGAAAGGAGATAGCAGTTAAAATGATGGCAGAGTCATCTGCCCACATGAATAAGCAATTTGTAACTGAGGTAGCACTCTTGTCACGTATTCATCATCGGAACTTGGTTCCTCTAACTGGATATTGCGAAGAAGAACATCAATGCATTCTGGTTTACGAGTACATGCACAATGGAACCTTACGCGATCACATACACGGTTCTACAACCCAGAAGCGCTTAGACTGGCAAACTCGCCTTCGCATTGCAGAAGATGCAGCTAAAGGTCTCGAGTACTTACATACTGGATGCAACCCTAGTATCATCCACCGGGACGTGAAAACAAGCAACATTCTCTTAGACATAAACATGAGAGCAAAAGTGTCGGATTTCGGATTGTCTAGGCAAGCAGAGGAGGATTTAACGCATATCTCAAGCGTGGCTCAGGGAACAGTAGGCTACCTTGATCCTGAGTATTATGCTAGTCAGCAATTAACTGAGAAAAGTGATGTGTACAGTTTTGGGGTTGTTCTTTTGGAGCTGATTTCAGGAAAAAAGCCAGTTTCAACAGAAGACTTTGGTTCTGAGTTGAACATTGTTCACTGGGCAAGATCCTTGATTCGTAGAGGAGATGTTGAAAGCATCATAGATCCTATCCTAGCAGGAAGTGTGGAAGTTGAGTCCATTTGGAGAATTGCTGAAGTAGCAATCCAGTGTGTTGAGCAACATGGAGTTTCCAGGCCAAGAATGCAGGAGATAATCGTGGCCGTTCAGGATGCTACGAAGATTGAAAAGGGCAGTGAATCCAACCAGAAAATATCACCCAGCAGTAGCAGTTCAAAAGCACAACCCTCCAGGAAAACTTTGCTAACAAGCTTTCTGGAAATTGAGAGCCCTGATTTGTCCAAGGATTGTTTAGTCCCATCTGCCAGACGAGATGATGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_4 LRR_8 Malectin_like Pkinase_Tyr

Protein Analysis

936

Amino Acids

104.64

Weight (kDa)

5.96

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 28 - 361 1.1e-80 Malectin-like domain
LRR_8 PF13855 438 - 495 2.6e-06 Leucine rich repeat
LRR_4 PF12799 461 - 494 2e-06 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 608 - 872 2.4e-50 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 609 - 811 9.7e-47 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67720
fragaria_vesca FvH4_4g24900 FvH4_4g24900 FvH4_4g24900
malus_domestica MD13G1108000.v1.1 MD16G1108100.v1.1
prunus_persica Prupe.1G238400_v2.0.a1 Prupe.1G238400_v2.0.a1
pyrus_communis pycom16g09210
rosa_chinensis RchiOBHm_Chr4g0431961
rosa_laevigata RLG00000006871
rosa_multiflora Rmu_sc0004965.1_g000014
rosa_roxburghii Rroxscaffold_5G00373400
rosa_rugosa Rorug04G0253600 Rorug04G0253700
rosa_samantha Rh4BG317900
rosa_wichuraiana Rw4G027040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 609
AccB1I GGYRCC 2 cut(s) 609, 682
AccB7I CCANNNNNTGG 1 cut(s) 369
AccI GTMKAC 1 cut(s) 720
AccII CGCG 3 cut(s) 767, 1048, 2064
AccIII TCCGGA 1 cut(s) 882
AciI CCGC 6 cut(s) 765, 767, 851, 1055, 1127, 1726
AclWI GGATC 6 cut(s) 614, 874, 1178, 2318, 2463, 2501
AcoI YGGCCR 1 cut(s) 2622
AcsI RAATTY 6 cut(s) 341, 577, 633, 887, 1497, 1602
AcuI CTGAAG 2 cut(s) 1127, 2577
AdeI CACNNNGTG 1 cut(s) 369
AfeI AGCGCT 1 cut(s) 2096
AfiI CCNNNNNNNGG 6 cut(s) 369, 826, 827, 1742, 1801, 1997
AjiI CACGTC 1 cut(s) 2194
AjnI CCWGG 8 cut(s) 60, 159, 174, 685, 820, 1437, 2594, 2711
AjuI GAANNNNNNNTTGG 4 cut(s) 878, 910, 1533, 1565
Alw21I GWGCWC 1 cut(s) 589
Alw26I GTCTC 3 cut(s) 208, 320, 2150
AlwI GGATC 6 cut(s) 614, 874, 1178, 2318, 2463, 2501
Ama87I CYCGRG 1 cut(s) 2147
Aor13HI TCCGGA 1 cut(s) 882
Aor51HI AGCGCT 1 cut(s) 2096
AoxI GGCC 4 cut(s) 506, 1052, 2597, 2622
ApeKI GCWGC 6 cut(s) 202, 566, 1097, 1717, 1818, 2135
ApoI RAATTY 6 cut(s) 341, 577, 633, 887, 1497, 1602
Asp700I GAANNNNTTC 1 cut(s) 1583
Asp718I GGTACC 1 cut(s) 609
AspLEI GCGC 3 cut(s) 872, 1193, 2097
AspS9I GGNCC 1 cut(s) 1052
AsuC2I CCSGG 3 cut(s) 293, 614, 2189
AsuHPI GGTGA 6 cut(s) 586, 1193, 1225, 1292, 1721, 2670
AsuII TTCGAA 1 cut(s) 1826
AsuNHI GCTAGC 1 cut(s) 1442
AvaI CYCGRG 1 cut(s) 2147
BaeGI GKGCMC 1 cut(s) 1726
BaeI ACNNNNGTAYC 2 cut(s) 2161, 2194
BanI GGYRCC 2 cut(s) 609, 682
BanII GRGCYC 1 cut(s) 2753
BbsI GAAGAC 2 cut(s) 333, 2436
Bbv12I GWGCWC 1 cut(s) 589
BbvCI CCTCAGC 1 cut(s) 503
BbvI GCAGC 6 cut(s) 189, 578, 1084, 1704, 1830, 2147
BccI CCATC 4 cut(s) 806, 1874, 1900, 2788
BceAI ACGGC 2 cut(s) 325, 2609
BcgI CGANNNNNNTGC 2 cut(s) 2223, 2257
BciT130I CCWGG 8 cut(s) 62, 161, 176, 687, 822, 1439, 2596, 2713
BclI TGATCA 1 cut(s) 420
BcnI CCSGG 3 cut(s) 293, 614, 2189
BcoDI GTCTC 3 cut(s) 208, 320, 2150
BfaI CTAG 8 cut(s) 570, 1400, 1443, 1815, 2175, 2259, 2340, 2516
BfmI CTRYAG 4 cut(s) 203, 796, 1307, 1503
BfoI RGCGCY 1 cut(s) 2098
BisI GCNGC 7 cut(s) 203, 567, 1055, 1098, 1718, 1819, 2136
BlpI GCTNAGC 1 cut(s) 791
BlsI GCNGC 7 cut(s) 204, 568, 1056, 1099, 1719, 1820, 2137
BmcAI AGTACT 2 cut(s) 248, 2153
BmeT110I CYCGRG 1 cut(s) 2147
BmgBI CACGTC 1 cut(s) 2194
BmgT120I GGNCC 1 cut(s) 1052
BmiI GGNNCC 4 cut(s) 611, 684, 1989, 2056
BmrI ACTGGG 2 cut(s) 483, 2470
BmsI GCATC 8 cut(s) 349, 382, 589, 1405, 2122, 2156, 2508, 2623
BmtI GCTAGC 1 cut(s) 1446
BmuI ACTGGG 2 cut(s) 483, 2470
BpiI GAAGAC 2 cut(s) 333, 2436
BpmI CTGGAG 2 cut(s) 1022, 2695
Bpu10I CCTNAGC 2 cut(s) 503, 2301
Bpu1102I GCTNAGC 1 cut(s) 791
Bpu14I TTCGAA 1 cut(s) 1826
BpuEI CTTGAG 1 cut(s) 2275
BpuMI CCSGG 3 cut(s) 293, 614, 2189
BsaAI YACGTR 2 cut(s) 1559, 1967
BsaBI GATNNNNATC 2 cut(s) 422, 645
BsaI GGTCTC 1 cut(s) 2150
BsaJI CCNNGG 9 cut(s) 60, 159, 160, 531, 765, 820, 1202, 1290, 2763
BsaWI WCCGGW 2 cut(s) 882, 1349
Bsc4I CCNNNNNNNGG 6 cut(s) 369, 826, 827, 1742, 1801, 1997
Bse3DI GCAATG 2 cut(s) 1074, 2121
Bse8I GATNNNNATC 2 cut(s) 422, 645
BseAI TCCGGA 1 cut(s) 882
BseBI CCWGG 8 cut(s) 62, 161, 176, 687, 822, 1439, 2596, 2713
BseDI CCNNGG 9 cut(s) 60, 159, 160, 531, 765, 820, 1202, 1290, 2763
BseGI GGATG 9 cut(s) 364, 550, 622, 721, 817, 1771, 2171, 2181, 2638
BseJI GATNNNNATC 2 cut(s) 422, 645
BseLI CCNNNNNNNGG 6 cut(s) 369, 826, 827, 1742, 1801, 1997
BseMI GCAATG 2 cut(s) 1074, 2121
BseMII CTCAG 9 cut(s) 24, 494, 782, 936, 1938, 2315, 2319, 2346, 2433
BseRI GAGGAG 4 cut(s) 289, 292, 2285, 2499
BseSI GKGCMC 1 cut(s) 1726
BseXI GCAGC 6 cut(s) 189, 578, 1084, 1704, 1830, 2147
BseYI CCCAGC 1 cut(s) 2681
BsgI GTGCAG 1 cut(s) 948
Bsh1236I CGCG 3 cut(s) 767, 1048, 2064
BshFI GGCC 4 cut(s) 508, 1054, 2599, 2624
BshNI GGYRCC 2 cut(s) 609, 682
BsiHKAI GWGCWC 1 cut(s) 589
BsiHKCI CYCGRG 1 cut(s) 2147
BsiSI CCGG 5 cut(s) 292, 614, 883, 1350, 2188
BslFI GGGAC 6 cut(s) 109, 142, 974, 1660, 2204, 2762
BslI CCNNNNNNNGG 6 cut(s) 369, 826, 827, 1742, 1801, 1997
BsmAI GTCTC 3 cut(s) 208, 320, 2150
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 6 cut(s) 109, 142, 974, 1660, 2204, 2762
BsmI GAATGC 3 cut(s) 955, 2025, 2610
BsnI GGCC 4 cut(s) 508, 1054, 2599, 2624
Bso31I GGTCTC 1 cut(s) 2150
BsoBI CYCGRG 1 cut(s) 2147
Bsp119I TTCGAA 1 cut(s) 1826
Bsp1286I GDGCHC 3 cut(s) 589, 1726, 2753
Bsp13I TCCGGA 1 cut(s) 882
Bsp1407I TGTACA 2 cut(s) 1467, 2370
Bsp1720I GCTNAGC 1 cut(s) 791
BspACI CCGC 6 cut(s) 765, 767, 851, 1055, 1127, 1726
BspANI GGCC 4 cut(s) 508, 1054, 2599, 2624
BspCNI CTCAG 9 cut(s) 23, 495, 783, 937, 1939, 2314, 2320, 2347, 2434
BspEI TCCGGA 1 cut(s) 882
BspFNI CGCG 3 cut(s) 767, 1048, 2064
BspLI GGNNCC 4 cut(s) 611, 684, 1989, 2056
BspMAI CTGCAG 3 cut(s) 207, 1311, 1507
BspOI GCTAGC 1 cut(s) 1446
BspPI GGATC 6 cut(s) 614, 874, 1178, 2318, 2463, 2501
BspT104I TTCGAA 1 cut(s) 1826
BspT107I GGYRCC 2 cut(s) 609, 682
BspTNI GGTCTC 1 cut(s) 2150
BsrDI GCAATG 2 cut(s) 1074, 2121
BsrGI TGTACA 2 cut(s) 1467, 2370
BssECI CCNNGG 9 cut(s) 60, 159, 160, 531, 765, 820, 1202, 1290, 2763
BssT1I CCWWGG 1 cut(s) 2763
Bst2UI CCWGG 8 cut(s) 62, 161, 176, 687, 822, 1439, 2596, 2713
Bst4CI ACNGT 8 cut(s) 268, 379, 1467, 1512, 1627, 2078, 2311, 2375
Bst6I CTCTTC 1 cut(s) 1800
BstAPI GCANNNNNTGC 1 cut(s) 2132
BstAUI TGTACA 2 cut(s) 1467, 2370
BstBAI YACGTR 2 cut(s) 1559, 1967
BstBI TTCGAA 1 cut(s) 1826
BstC8I GCNNGC 6 cut(s) 1444, 1448, 1722, 1726, 1780, 2265
BstDSI CCRYGG 4 cut(s) 531, 765, 1202, 1290
BstF5I GGATG 9 cut(s) 364, 550, 622, 721, 817, 1771, 2171, 2181, 2638
BstFNI CGCG 3 cut(s) 767, 1048, 2064
BstH2I RGCGCY 1 cut(s) 2098
BstHHI GCGC 3 cut(s) 872, 1193, 2097
BstMAI GTCTC 3 cut(s) 208, 320, 2150
BstNI CCWGG 8 cut(s) 62, 161, 176, 687, 822, 1439, 2596, 2713
BstNSI RCATGY 2 cut(s) 148, 2047
BstSFI CTRYAG 4 cut(s) 203, 796, 1307, 1503
BstSLI GKGCMC 1 cut(s) 1726
BstSNI TACGTA 1 cut(s) 1559
BstUI CGCG 3 cut(s) 767, 1048, 2064
BstV1I GCAGC 6 cut(s) 189, 578, 1084, 1704, 1830, 2147
BstV2I GAAGAC 2 cut(s) 333, 2436
BstX2I RGATCY 3 cut(s) 879, 2468, 2506
BstXI CCANNNNNNTGG 1 cut(s) 1203
BstYI RGATCY 3 cut(s) 879, 2468, 2506
BsuRI GGCC 4 cut(s) 508, 1054, 2599, 2624
BtgI CCRYGG 4 cut(s) 531, 765, 1202, 1290
BtrI CACGTC 1 cut(s) 2194
BtsCI GGATG 9 cut(s) 364, 550, 622, 721, 817, 1771, 2171, 2181, 2638
BtsI GCAGTG 2 cut(s) 374, 2662
BtsIMutI CAGTG 7 cut(s) 120, 264, 374, 1495, 2458, 2576, 2662
Cac8I GCNNGC 6 cut(s) 1444, 1448, 1722, 1726, 1780, 2265
CfoI GCGC 3 cut(s) 872, 1193, 2097
Cfr13I GGNCC 1 cut(s) 1052
Cfr42I CCGCGG 1 cut(s) 768
CseI GACGC 1 cut(s) 225
CsiI ACCWGGT 1 cut(s) 685
CspCI CAANNNNNGTGG 2 cut(s) 1399, 1434
CviAII CATG 9 cut(s) 132, 145, 154, 988, 1150, 1927, 2044, 2227, 2585
DraI TTTAAA 2 cut(s) 1386, 1534
DraIII CACNNNGTG 1 cut(s) 369
EaeI YGGCCR 1 cut(s) 2622
Eam1104I CTCTTC 1 cut(s) 1800
EarI CTCTTC 1 cut(s) 1800
Eco105I TACGTA 1 cut(s) 1559
Eco130I CCWWGG 1 cut(s) 2763
Eco147I AGGCCT 1 cut(s) 508
Eco24I GRGCYC 1 cut(s) 2753
Eco31I GGTCTC 1 cut(s) 2150
Eco47III AGCGCT 1 cut(s) 2096
Eco57I CTGAAG 2 cut(s) 1127, 2577
Eco88I CYCGRG 1 cut(s) 2147
EcoRII CCWGG 8 cut(s) 60, 159, 174, 685, 820, 1437, 2594, 2711
EcoT14I CCWWGG 1 cut(s) 2763
EcoT22I ATGCAT 1 cut(s) 2027
EcoT38I GRGCYC 1 cut(s) 2753
ErhI CCWWGG 1 cut(s) 2763
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 9 cut(s) 135, 148, 157, 991, 1153, 1930, 2047, 2230, 2588
FaqI GGGAC 6 cut(s) 109, 142, 974, 1660, 2204, 2762
FatI CATG 9 cut(s) 131, 144, 153, 987, 1149, 1926, 2043, 2226, 2584
FauI CCCGC 1 cut(s) 1733
FauNDI CATATG 2 cut(s) 406, 625
FbaI TGATCA 1 cut(s) 420
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 7 cut(s) 203, 567, 1055, 1098, 1718, 1819, 2136
FokI GGATG 9 cut(s) 371, 557, 629, 728, 824, 1778, 2168, 2178, 2645
FriOI GRGCYC 1 cut(s) 2753
Fsp4HI GCNGC 7 cut(s) 203, 567, 1055, 1098, 1718, 1819, 2136
FspBI CTAG 8 cut(s) 570, 1400, 1443, 1815, 2175, 2259, 2340, 2516
GlaI GCGC 3 cut(s) 871, 1192, 2096
GluI GCNGC 7 cut(s) 203, 567, 1055, 1098, 1718, 1819, 2136
GsaI CCCAGC 1 cut(s) 2685
GsuI CTGGAG 2 cut(s) 1022, 2695
HaeII RGCGCY 1 cut(s) 2098
HaeIII GGCC 4 cut(s) 508, 1054, 2599, 2624
HapII CCGG 5 cut(s) 292, 614, 883, 1350, 2188
HgaI GACGC 1 cut(s) 225
HhaI GCGC 3 cut(s) 872, 1193, 2097
Hin1II CATG 9 cut(s) 135, 148, 157, 991, 1153, 1930, 2047, 2230, 2588
Hin6I GCGC 3 cut(s) 870, 1191, 2095
HinP1I GCGC 3 cut(s) 870, 1191, 2095
HincII GTYRAC 1 cut(s) 1317
HindII GTYRAC 1 cut(s) 1317
HindIII AAGCTT 3 cut(s) 1453, 1846, 2731
HpaII CCGG 5 cut(s) 292, 614, 883, 1350, 2188
HphI GGTGA 6 cut(s) 586, 1193, 1225, 1292, 1721, 2670
Hpy166II GTNNAC 6 cut(s) 721, 1317, 1424, 2035, 2371, 2458
Hpy8I GTNNAC 6 cut(s) 721, 1317, 1424, 2035, 2371, 2458
Hpy99I CGWCG 1 cut(s) 439
HpyAV CCTTC 6 cut(s) 1172, 1270, 1440, 1746, 1763, 2126
HpyCH4III ACNGT 8 cut(s) 268, 379, 1467, 1512, 1627, 2078, 2311, 2375
HpyCH4IV ACGT 4 cut(s) 257, 1558, 1966, 2193
HpySE526I ACGT 4 cut(s) 257, 1558, 1966, 2193
Hsp92II CATG 9 cut(s) 135, 148, 157, 991, 1153, 1930, 2047, 2230, 2588
HspAI GCGC 3 cut(s) 870, 1191, 2095
Kpn2I TCCGGA 1 cut(s) 882
KpnI GGTACC 1 cut(s) 613
Ksp22I TGATCA 1 cut(s) 420
KspI CCGCGG 1 cut(s) 768
LmnI GCTCC 7 cut(s) 499, 592, 898, 1198, 1229, 1294, 2395
Lsp1109I GCAGC 6 cut(s) 189, 578, 1084, 1704, 1830, 2147
LweI GCATC 8 cut(s) 349, 382, 589, 1405, 2122, 2156, 2508, 2623
MabI ACCWGGT 1 cut(s) 685
MaeI CTAG 8 cut(s) 570, 1400, 1443, 1815, 2175, 2259, 2340, 2516
MaeII ACGT 4 cut(s) 257, 1558, 1966, 2193
MaeIII GTNAC 6 cut(s) 101, 379, 1337, 1709, 1942, 1962
MboII GAAGA 8 cut(s) 338, 1661, 1817, 2021, 2024, 2141, 2441, 2653
MflI RGATCY 3 cut(s) 879, 2468, 2506
MhlI GDGCHC 3 cut(s) 589, 1726, 2753
MlyI GAGTC 5 cut(s) 1006, 1642, 1706, 1922, 2546
MmeI TCCRAC 7 cut(s) 417, 655, 876, 1220, 1591, 2223, 2688
Mph1103I ATGCAT 1 cut(s) 2027
MroI TCCGGA 1 cut(s) 882
MroXI GAANNNNTTC 1 cut(s) 1583
MslI CAYNNNNRTG 2 cut(s) 992, 2225
MspA1I CMGCKG 1 cut(s) 767
MspI CCGG 5 cut(s) 292, 614, 883, 1350, 2188
Mva1269I GAATGC 3 cut(s) 955, 2025, 2610
MvaI CCWGG 8 cut(s) 62, 161, 176, 687, 822, 1439, 2596, 2713
MvnI CGCG 3 cut(s) 767, 1048, 2064
NciI CCSGG 3 cut(s) 293, 614, 2189
NdeI CATATG 2 cut(s) 406, 625
NheI GCTAGC 1 cut(s) 1442
NlaIII CATG 9 cut(s) 135, 148, 157, 991, 1153, 1930, 2047, 2230, 2588
NlaIV GGNNCC 4 cut(s) 611, 684, 1989, 2056
NmeAIII GCCGAG 2 cut(s) 890, 1005
NmuCI GTSAC 3 cut(s) 379, 1709, 1962
NsiI ATGCAT 1 cut(s) 2027
NspI RCATGY 2 cut(s) 148, 2047
NspV TTCGAA 1 cut(s) 1826
PaeR7I CTCGAG 1 cut(s) 2147
PasI CCCWGGG 1 cut(s) 160
PceI AGGCCT 1 cut(s) 508
PctI GAATGC 3 cut(s) 955, 2025, 2610
PdmI GAANNNNTTC 1 cut(s) 1583
PfeI GAWTC 9 cut(s) 150, 440, 514, 641, 699, 1040, 1242, 2476, 2660
PflMI CCANNNNNTGG 1 cut(s) 369
PfoI TCCNGGA 1 cut(s) 2711
PkrI GCNGC 7 cut(s) 204, 568, 1056, 1099, 1719, 1820, 2137
PleI GAGTC 5 cut(s) 1005, 1642, 1706, 1921, 2545
PpsI GAGTC 5 cut(s) 1005, 1642, 1706, 1921, 2545
Ppu21I YACGTR 2 cut(s) 1559, 1967
Psp6I CCWGG 8 cut(s) 60, 159, 174, 685, 820, 1437, 2594, 2711
PspFI CCCAGC 1 cut(s) 2681
PspGI CCWGG 8 cut(s) 60, 159, 174, 685, 820, 1437, 2594, 2711
PspN4I GGNNCC 4 cut(s) 611, 684, 1989, 2056
PspPI GGNCC 1 cut(s) 1052
PsrI GAACNNNNNNTAC 2 cut(s) 1455, 1487
PstI CTGCAG 3 cut(s) 207, 1311, 1507
PsuI RGATCY 3 cut(s) 879, 2468, 2506
RseI CAYNNNNRTG 2 cut(s) 992, 2225
SacII CCGCGG 1 cut(s) 768
SatI GCNGC 7 cut(s) 203, 567, 1055, 1098, 1718, 1819, 2136
Sau96I GGNCC 1 cut(s) 1052
SbfI CCTGCAGG 1 cut(s) 1507
ScaI AGTACT 2 cut(s) 248, 2153
SchI GAGTC 5 cut(s) 1006, 1642, 1706, 1922, 2546
SdaI CCTGCAGG 1 cut(s) 1507
SduI GDGCHC 3 cut(s) 589, 1726, 2753
SexAI ACCWGGT 1 cut(s) 685
SfaNI GCATC 8 cut(s) 349, 382, 589, 1405, 2122, 2156, 2508, 2623
SfcI CTRYAG 4 cut(s) 203, 796, 1307, 1503
Sfr274I CTCGAG 1 cut(s) 2147
Sfr303I CCGCGG 1 cut(s) 768
SfuI TTCGAA 1 cut(s) 1826
SgrBI CCGCGG 1 cut(s) 768
SlaI CTCGAG 1 cut(s) 2147
SmiI ATTTAAAT 1 cut(s) 1534
SmiMI CAYNNNNRTG 2 cut(s) 992, 2225
SmlI CTYRAG 2 cut(s) 2147, 2290
SmoI CTYRAG 2 cut(s) 2147, 2290
SnaBI TACGTA 1 cut(s) 1559
Sse8387I CCTGCAGG 1 cut(s) 1507
SseBI AGGCCT 1 cut(s) 508
SsiI CCGC 6 cut(s) 765, 767, 851, 1055, 1127, 1726
SspI AATATT 1 cut(s) 940
SspMI CTAG 8 cut(s) 570, 1400, 1443, 1815, 2175, 2259, 2340, 2516
StuI AGGCCT 1 cut(s) 508
StyI CCWWGG 1 cut(s) 2763
SwaI ATTTAAAT 1 cut(s) 1534
TaaI ACNGT 8 cut(s) 268, 379, 1467, 1512, 1627, 2078, 2311, 2375
TaiI ACGT 4 cut(s) 260, 1561, 1969, 2196
TaqI TCGA 8 cut(s) 180, 345, 447, 491, 521, 832, 1826, 2148
TaqII GACCGA 1 cut(s) 1121
TatI WGTACW 6 cut(s) 246, 704, 1467, 2040, 2151, 2370
TauI GCSGC 1 cut(s) 1057
TfiI GAWTC 9 cut(s) 150, 440, 514, 641, 699, 1040, 1242, 2476, 2660
TscAI CASTG 7 cut(s) 127, 271, 374, 1495, 2465, 2576, 2662
TseFI GTSAC 3 cut(s) 379, 1709, 1962
TseI GCWGC 6 cut(s) 202, 566, 1097, 1717, 1818, 2135
Tsp45I GTSAC 3 cut(s) 379, 1709, 1962
TspGWI ACGGA 3 cut(s) 471, 548, 1191
TspRI CASTG 7 cut(s) 127, 271, 374, 1495, 2465, 2576, 2662
Van91I CCANNNNNTGG 1 cut(s) 369
XapI RAATTY 6 cut(s) 341, 577, 633, 887, 1497, 1602
XbaI TCTAGA 1 cut(s) 1399
XceI RCATGY 2 cut(s) 148, 2047
XhoI CTCGAG 1 cut(s) 2147
XmiI GTMKAC 1 cut(s) 720
XmnI GAANNNNTTC 1 cut(s) 1583
XspI CTAG 8 cut(s) 570, 1400, 1443, 1815, 2175, 2259, 2340, 2516
ZrmI AGTACT 2 cut(s) 248, 2153
Zsp2I ATGCAT 1 cut(s) 2027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.