pycom16g10530

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0019744
Physical Location & Seq
Forward (+)
7204783 .. 7206626
1844 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 273 bp
ATGGAGGCCTTTACTTGGCTAAAGAGAAAAGGGTTTGGGACAGCTGACCAAGTCCCCTTAAGAGAGGAGGTTATGTGCCTGCTTGCTGAGCTAGAATTCAACATGCACTGTGGACATTGTGCAGATGATGTCAAGAAATGCTGCAGTAAGACGAAAGGAGTTGAAAGTGTTGAGGTTGATCAAGTGAACAAAGTAGTGACAGTGGAAGGAAGATTTCGTCATAAAAAGCTTCTCAAATGCCTCCGAAGGGCTAACAAGATTGTTAAAGGCTAG

Protein Analysis

91

Amino Acids

10.27

Weight (kDa)

9.15

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 33 - 85 3.2e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019744)

Species Orthologous Gene IDs
malus_domestica MD13G1122700.v1.1
prunus_persica Prupe.1G222500_v2.0.a1
pyrus_communis pycom16g10530
rosa_roxburghii Rroxscaffold_5G00371540
rosa_samantha Rh4AG294400 Rh4BG300400 Rh4DG297500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 95
AfiI CCNNNNNNNGG 2 cut(s) 15, 247
AflII CTTAAG 1 cut(s) 58
AgsI TTSAA 2 cut(s) 100, 164
AluBI AGCT 3 cut(s) 44, 91, 229
AluI AGCT 3 cut(s) 44, 91, 229
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 1 cut(s) 141
ApoI RAATTY 1 cut(s) 95
BbvI GCAGC 1 cut(s) 128
BclI TGATCA 1 cut(s) 178
BfaI CTAG 2 cut(s) 92, 271
BfmI CTRYAG 1 cut(s) 142
BfrI CTTAAG 1 cut(s) 58
BisI GCNGC 1 cut(s) 142
BlpI GCTNAGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 143
Bpu1102I GCTNAGC 1 cut(s) 87
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 247
BseLI CCNNNNNNNGG 2 cut(s) 15, 247
BseMII CTCAG 1 cut(s) 78
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 1 cut(s) 128
BsgI GTGCAG 1 cut(s) 141
BshFI GGCC 1 cut(s) 8
BslFI GGGAC 2 cut(s) 38, 52
BslI CCNNNNNNNGG 2 cut(s) 15, 247
BsmFI GGGAC 2 cut(s) 38, 52
BsnI GGCC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 178
Bsp1720I GCTNAGC 1 cut(s) 87
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 79
BspMAI CTGCAG 1 cut(s) 146
BspTI CTTAAG 1 cut(s) 58
BssMI GATC 1 cut(s) 178
Bst4CI ACNGT 2 cut(s) 110, 202
BstAFI CTTAAG 1 cut(s) 58
BstC8I GCNNGC 2 cut(s) 80, 84
BstDEI CTNAG 1 cut(s) 87
BstKTI GATC 1 cut(s) 181
BstMBI GATC 1 cut(s) 178
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstNSI RCATGY 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 142
BstV1I GCAGC 1 cut(s) 128
BsuRI GGCC 1 cut(s) 8
BtsIMutI CAGTG 2 cut(s) 106, 207
Cac8I GCNNGC 2 cut(s) 80, 84
CviAII CATG 1 cut(s) 103
CviJI RGCY 7 cut(s) 8, 19, 44, 91, 229, 251, 270
CviKI_1 RGCY 7 cut(s) 8, 19, 44, 91, 229, 251, 270
DdeI CTNAG 1 cut(s) 87
DpnI GATC 1 cut(s) 180
DpnII GATC 1 cut(s) 178
Eco147I AGGCCT 1 cut(s) 8
EcoRI GAATTC 1 cut(s) 95
FaeI CATG 1 cut(s) 106
FaiI YATR 3 cut(s) 74, 104, 222
FaqI GGGAC 2 cut(s) 38, 52
FatI CATG 1 cut(s) 102
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 1 cut(s) 142
Fsp4HI GCNGC 1 cut(s) 142
FspBI CTAG 2 cut(s) 92, 271
GluI GCNGC 1 cut(s) 142
HaeIII GGCC 1 cut(s) 8
Hin1II CATG 1 cut(s) 106
HindIII AAGCTT 1 cut(s) 227
Hpy166II GTNNAC 2 cut(s) 113, 187
Hpy188I TCNGA 1 cut(s) 245
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 2 cut(s) 113, 187
HpyAV CCTTC 2 cut(s) 200, 240
HpyCH4III ACNGT 2 cut(s) 110, 202
HpyCH4V TGCA 3 cut(s) 106, 122, 144
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpyF3I CTNAG 1 cut(s) 87
Hsp92II CATG 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 1 cut(s) 178
LpnPI CCDG 1 cut(s) 92
Lsp1109I GCAGC 1 cut(s) 128
MaeI CTAG 2 cut(s) 92, 271
MaeIII GTNAC 1 cut(s) 196
MalI GATC 1 cut(s) 180
MboI GATC 1 cut(s) 178
MboII GAAGA 1 cut(s) 222
MluCI AATT 1 cut(s) 95
MnlI CCTC 4 cut(s) 58, 61, 166, 251
MseI TTAA 2 cut(s) 59, 264
MspA1I CMGCKG 1 cut(s) 44
MspCI CTTAAG 1 cut(s) 58
MwoI GCNNNNNNNGC 1 cut(s) 88
NdeII GATC 1 cut(s) 178
NlaIII CATG 1 cut(s) 106
NmuCI GTSAC 1 cut(s) 196
NspI RCATGY 1 cut(s) 106
PceI AGGCCT 1 cut(s) 8
PflFI GACNNNGTC 1 cut(s) 50
PkrI GCNGC 1 cut(s) 143
PstI CTGCAG 1 cut(s) 146
PsyI GACNNNGTC 1 cut(s) 50
PvuII CAGCTG 1 cut(s) 44
SaqAI TTAA 2 cut(s) 59, 264
SatI GCNGC 1 cut(s) 142
Sau3AI GATC 1 cut(s) 178
SetI ASST 5 cut(s) 46, 72, 93, 177, 231
SfcI CTRYAG 1 cut(s) 142
SgeI CNNG 9 cut(s) 27, 62, 91, 95, 104, 115, 145, 194, 268
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
Sse9I AATT 1 cut(s) 95
SseBI AGGCCT 1 cut(s) 8
SspMI CTAG 2 cut(s) 92, 271
StuI AGGCCT 1 cut(s) 8
TaaI ACNGT 2 cut(s) 110, 202
TasI AATT 1 cut(s) 95
Tru1I TTAA 2 cut(s) 59, 264
Tru9I TTAA 2 cut(s) 59, 264
TscAI CASTG 2 cut(s) 113, 207
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 1 cut(s) 141
Tsp45I GTSAC 1 cut(s) 196
TspRI CASTG 2 cut(s) 113, 207
Tth111I GACNNNGTC 1 cut(s) 50
Vha464I CTTAAG 1 cut(s) 58
XapI RAATTY 1 cut(s) 95
XceI RCATGY 1 cut(s) 106
XspI CTAG 2 cut(s) 92, 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.