pycom16g11550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0015405
Physical Location & Seq
Forward (+)
8047565 .. 8049580
2016 bp
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UTR
Exon/CDS
Intron
pycom16g11550.4

Sequence Viewer

Length: 615 bp
ATGGCGTTGCAGACCAGTACCAGCAGCAATGTTCTGCTCCTGCAATCCTCCCCGGTTCCGTGTCGGGTCGATTCCTTCCGGATCCGAACTCGAGTGGTCGGAGCTGGAATCTGGGGTTGGAGGAGAACGGGAGGAGGAGGAAGGGTTTGTATGATCGCATGCAAGAGTAGCAGCGGTGGCGATGTGCCCAATTCGAATTATGTGGTGCCTCTAGACAAATCGTTTTCGTATTCCAACTCGTCCCTCATCACTCGCCCTCTCACTGAAATCCTCAGAGATCTCAACAAGCGAATCCCTGATAACATCATCCAGACGGCAGCTGAAAGTCCCTCCTCCACCTTCATCCCCTGGTACCATGCCAACCGGATGTTGAGCTTCTACGCTGCTGGATGGTGCGGAGAAGTCCGTGATGTTATATTTTCTGACAATGGAAGCGTGACTGTGGTATACCGTGTCACCGTACGTGGATCTGATGGAGAGGCACACCGTGAATCAAGTGGAACAGTAACACCCAGTGACGATATTGTCGATCCAGTTGCTGCAGCTGAGCAACTTGCTTTCTGCAGAGCGTGTGCTCGGTTTGGCCTTGGCCTGTATCTGTATCATGAAGATTAG

Protein Analysis

205

Amino Acids

22.22

Weight (kDa)

7.61

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47870
fragaria_vesca FvH4_4g21981
prunus_persica Prupe.1G209900_v2.0.a1 Prupe.1G209900_v2.0.a1 Prupe.1G209900_v2.0.a1
pyrus_communis pycom16g11550
rosa_chinensis RchiOBHm_Chr4g0428171
rosa_laevigata RLG00000007173
rosa_multiflora Rmu_sc0004940.1_g000007 Rmu_sc0009599.1_g000007
rosa_roxburghii Rroxscaffold_5G00369900
rosa_rugosa Rorug04G0223200
rosa_samantha Rh4AG280500 Rh4BG286000 Rh4DG283000
rosa_wichuraiana Rw4G024360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 524
Acc65I GGTACC 1 cut(s) 351
AccB1I GGYRCC 2 cut(s) 205, 351
AccI GTMKAC 1 cut(s) 447
AccIII TCCGGA 1 cut(s) 78
AciI CCGC 2 cut(s) 174, 396
AclWI GGATC 4 cut(s) 76, 89, 475, 524
AdeI CACNNNGTG 2 cut(s) 488, 515
AfaI GTAC 3 cut(s) 19, 353, 462
AjnI CCWGG 1 cut(s) 347
AjuI GAANNNNNNNTTGG 2 cut(s) 100, 132
AluBI AGCT 4 cut(s) 104, 320, 375, 545
AluI AGCT 4 cut(s) 104, 320, 375, 545
Alw21I GWGCWC 1 cut(s) 577
AlwI GGATC 4 cut(s) 76, 89, 475, 524
AlwNI CAGNNNCTG 1 cut(s) 539
Ama87I CYCGRG 1 cut(s) 90
Aor13HI TCCGGA 1 cut(s) 78
AoxI GGCC 2 cut(s) 583, 589
ApeKI GCWGC 6 cut(s) 24, 171, 317, 383, 539, 542
Asp718I GGTACC 1 cut(s) 351
AsuC2I CCSGG 1 cut(s) 53
AsuHPI GGTGA 1 cut(s) 448
AsuII TTCGAA 1 cut(s) 194
AvaI CYCGRG 1 cut(s) 90
BaeGI GKGCMC 1 cut(s) 189
BamHI GGATCC 1 cut(s) 81
BanI GGYRCC 2 cut(s) 205, 351
Bbv12I GWGCWC 1 cut(s) 577
BbvI GCAGC 6 cut(s) 36, 183, 329, 370, 526, 554
BccI CCATC 2 cut(s) 384, 467
BceAI ACGGC 1 cut(s) 330
BcgI CGANNNNNNTGC 2 cut(s) 518, 552
BciT130I CCWGG 1 cut(s) 349
BcnI CCSGG 1 cut(s) 53
BfaI CTAG 1 cut(s) 212
BfmI CTRYAG 2 cut(s) 540, 562
BglII AGATCT 1 cut(s) 277
BisI GCNGC 6 cut(s) 25, 172, 318, 384, 540, 543
BlpI GCTNAGC 1 cut(s) 546
BlsI GCNGC 6 cut(s) 26, 173, 319, 385, 541, 544
Bme1390I CCNGG 2 cut(s) 53, 349
BmeT110I CYCGRG 1 cut(s) 90
BmiI GGNNCC 4 cut(s) 57, 83, 207, 353
BmrFI CCNGG 2 cut(s) 53, 349
BmrI ACTGGG 1 cut(s) 507
BmuI ACTGGG 1 cut(s) 507
Bpu1102I GCTNAGC 1 cut(s) 546
Bpu14I TTCGAA 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 53
BsaAI YACGTR 1 cut(s) 464
BsaJI CCNNGG 3 cut(s) 51, 347, 586
BsaWI WCCGGW 2 cut(s) 78, 363
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
Bse1I ACTGG 3 cut(s) 15, 513, 533
Bse3DI GCAATG 1 cut(s) 34
BseAI TCCGGA 1 cut(s) 78
BseBI CCWGG 1 cut(s) 349
BseDI CCNNGG 3 cut(s) 51, 347, 586
BseGI GGATG 4 cut(s) 306, 342, 372, 395
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 2 cut(s) 286, 537
BseNI ACTGG 3 cut(s) 15, 513, 533
BseRI GAGGAG 4 cut(s) 136, 147, 150, 322
BseSI GKGCMC 1 cut(s) 189
BseXI GCAGC 6 cut(s) 36, 183, 329, 370, 526, 554
BshFI GGCC 2 cut(s) 585, 591
BshNI GGYRCC 2 cut(s) 205, 351
BsiHKAI GWGCWC 1 cut(s) 577
BsiHKCI CYCGRG 1 cut(s) 90
BsiSI CCGG 3 cut(s) 53, 79, 364
BsiWI CGTACG 1 cut(s) 460
BslFI GGGAC 2 cut(s) 226, 312
BsmFI GGGAC 2 cut(s) 226, 312
BsnI GGCC 2 cut(s) 585, 591
BsoBI CYCGRG 1 cut(s) 90
Bsp119I TTCGAA 1 cut(s) 194
Bsp1286I GDGCHC 2 cut(s) 189, 577
Bsp13I TCCGGA 1 cut(s) 78
Bsp143I GATC 5 cut(s) 81, 153, 277, 467, 529
Bsp1720I GCTNAGC 1 cut(s) 546
BspACI CCGC 2 cut(s) 174, 396
BspANI GGCC 2 cut(s) 585, 591
BspCNI CTCAG 2 cut(s) 285, 538
BspEI TCCGGA 1 cut(s) 78
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 4 cut(s) 57, 83, 207, 353
BspMAI CTGCAG 2 cut(s) 544, 566
BspPI GGATC 4 cut(s) 76, 89, 475, 524
BspT104I TTCGAA 1 cut(s) 194
BspT107I GGYRCC 2 cut(s) 205, 351
BsrDI GCAATG 1 cut(s) 34
BsrI ACTGG 3 cut(s) 15, 513, 533
BssECI CCNNGG 3 cut(s) 51, 347, 586
BssMI GATC 5 cut(s) 81, 153, 277, 467, 529
BssNAI GTATAC 1 cut(s) 448
BssT1I CCWWGG 1 cut(s) 586
Bst1107I GTATAC 1 cut(s) 448
Bst2UI CCWGG 1 cut(s) 349
Bst4CI ACNGT 5 cut(s) 442, 452, 460, 488, 505
BstBAI YACGTR 1 cut(s) 464
BstBI TTCGAA 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 2 cut(s) 272, 546
BstF5I GGATG 4 cut(s) 306, 342, 372, 395
BstKTI GATC 5 cut(s) 84, 156, 280, 470, 532
BstMBI GATC 5 cut(s) 81, 153, 277, 467, 529
BstMWI GCNNNNNNNGC 2 cut(s) 168, 177
BstNI CCWGG 1 cut(s) 349
BstNSI RCATGY 1 cut(s) 162
BstSCI CCNGG 2 cut(s) 51, 347
BstSFI CTRYAG 2 cut(s) 540, 562
BstSLI GKGCMC 1 cut(s) 189
BstV1I GCAGC 6 cut(s) 36, 183, 329, 370, 526, 554
BstX2I RGATCY 3 cut(s) 81, 277, 467
BstYI RGATCY 3 cut(s) 81, 277, 467
BstZ17I GTATAC 1 cut(s) 448
BsuRI GGCC 2 cut(s) 585, 591
BtgZI GCGATG 1 cut(s) 195
BtsCI GGATG 4 cut(s) 306, 342, 372, 395
BtsIMutI CAGTG 2 cut(s) 261, 520
Cac8I GCNNGC 1 cut(s) 160
CaiI CAGNNNCTG 1 cut(s) 539
CciI TCATGA 1 cut(s) 604
Csp6I GTAC 3 cut(s) 18, 352, 461
CviAII CATG 3 cut(s) 159, 356, 605
CviJI RGCY 6 cut(s) 104, 320, 375, 545, 585, 591
CviKI_1 RGCY 6 cut(s) 104, 320, 375, 545, 585, 591
CviQI GTAC 3 cut(s) 18, 352, 461
DdeI CTNAG 2 cut(s) 272, 546
DpnI GATC 5 cut(s) 83, 155, 279, 469, 531
DpnII GATC 5 cut(s) 81, 153, 277, 467, 529
DraIII CACNNNGTG 2 cut(s) 488, 515
DrdI GACNNNNNNGTC 1 cut(s) 524
DseDI GACNNNNNNGTC 1 cut(s) 524
Eco130I CCWWGG 1 cut(s) 586
Eco88I CYCGRG 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 347
EcoT14I CCWWGG 1 cut(s) 586
ErhI CCWWGG 1 cut(s) 586
FaeI CATG 3 cut(s) 162, 359, 608
FaiI YATR 7 cut(s) 152, 160, 201, 357, 416, 448, 606
FaqI GGGAC 2 cut(s) 226, 312
FatI CATG 3 cut(s) 158, 355, 604
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 6 cut(s) 25, 172, 318, 384, 540, 543
FokI GGATG 4 cut(s) 293, 329, 379, 402
Fsp4HI GCNGC 6 cut(s) 25, 172, 318, 384, 540, 543
FspBI CTAG 1 cut(s) 212
GluI GCNGC 6 cut(s) 25, 172, 318, 384, 540, 543
HaeIII GGCC 2 cut(s) 585, 591
HapII CCGG 3 cut(s) 53, 79, 364
Hin1II CATG 3 cut(s) 162, 359, 608
HinfI GANTC 4 cut(s) 71, 108, 291, 491
HpaII CCGG 3 cut(s) 53, 79, 364
HphI GGTGA 1 cut(s) 448
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 5 cut(s) 86, 101, 275, 424, 472
Hpy188III TCNNGA 4 cut(s) 79, 212, 310, 605
Hpy8I GTNNAC 1 cut(s) 448
HpyAV CCTTC 3 cut(s) 85, 135, 349
HpyCH4III ACNGT 5 cut(s) 442, 452, 460, 488, 505
HpyCH4IV ACGT 1 cut(s) 463
HpyCH4V TGCA 5 cut(s) 10, 43, 162, 542, 564
HpyF10VI GCNNNNNNNGC 2 cut(s) 168, 177
HpyF3I CTNAG 2 cut(s) 272, 546
HpySE526I ACGT 1 cut(s) 463
Hsp92II CATG 3 cut(s) 162, 359, 608
Kpn2I TCCGGA 1 cut(s) 78
KpnI GGTACC 1 cut(s) 355
Kzo9I GATC 5 cut(s) 81, 153, 277, 467, 529
LmnI GCTCC 2 cut(s) 42, 101
Lsp1109I GCAGC 6 cut(s) 36, 183, 329, 370, 526, 554
MaeI CTAG 1 cut(s) 212
MaeII ACGT 1 cut(s) 463
MaeIII GTNAC 4 cut(s) 436, 454, 505, 515
MalI GATC 5 cut(s) 83, 155, 279, 469, 531
MboI GATC 5 cut(s) 81, 153, 277, 467, 529
MflI RGATCY 3 cut(s) 81, 277, 467
MhlI GDGCHC 2 cut(s) 189, 577
MluCI AATT 2 cut(s) 190, 196
MmeI TCCRAC 3 cut(s) 79, 98, 258
MroI TCCGGA 1 cut(s) 78
MspA1I CMGCKG 3 cut(s) 174, 320, 545
MspI CCGG 3 cut(s) 53, 79, 364
MspR9I CCNGG 2 cut(s) 53, 349
MvaI CCWGG 1 cut(s) 349
MwoI GCNNNNNNNGC 2 cut(s) 168, 177
NciI CCSGG 1 cut(s) 53
NdeII GATC 5 cut(s) 81, 153, 277, 467, 529
NlaIII CATG 3 cut(s) 162, 359, 608
NlaIV GGNNCC 4 cut(s) 57, 83, 207, 353
NmuCI GTSAC 3 cut(s) 436, 454, 515
NspI RCATGY 1 cut(s) 162
NspV TTCGAA 1 cut(s) 194
PaeI GCATGC 1 cut(s) 162
PaeR7I CTCGAG 1 cut(s) 90
PagI TCATGA 1 cut(s) 604
PcsI WCGNNNNNNNCGW 1 cut(s) 525
PfeI GAWTC 4 cut(s) 71, 108, 291, 491
Pfl23II CGTACG 1 cut(s) 460
PkrI GCNGC 6 cut(s) 26, 173, 319, 385, 541, 544
Ppu21I YACGTR 1 cut(s) 464
Psp6I CCWGG 1 cut(s) 347
PspGI CCWGG 1 cut(s) 347
PspLI CGTACG 1 cut(s) 460
PspN4I GGNNCC 4 cut(s) 57, 83, 207, 353
PspXI VCTCGAGB 1 cut(s) 90
PstI CTGCAG 2 cut(s) 544, 566
PstNI CAGNNNCTG 1 cut(s) 539
PsuI RGATCY 3 cut(s) 81, 277, 467
PvuII CAGCTG 2 cut(s) 320, 545
RsaI GTAC 3 cut(s) 19, 353, 462
RsaNI GTAC 3 cut(s) 18, 352, 461
SatI GCNGC 6 cut(s) 25, 172, 318, 384, 540, 543
Sau3AI GATC 5 cut(s) 81, 153, 277, 467, 529
ScrFI CCNGG 2 cut(s) 53, 349
SduI GDGCHC 2 cut(s) 189, 577
SetI ASST 6 cut(s) 106, 322, 341, 377, 466, 547
SfcI CTRYAG 2 cut(s) 540, 562
Sfr274I CTCGAG 1 cut(s) 90
SfuI TTCGAA 1 cut(s) 194
SlaI CTCGAG 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 90
SmoI CTYRAG 1 cut(s) 90
SphI GCATGC 1 cut(s) 162
Sse9I AATT 2 cut(s) 190, 196
SsiI CCGC 2 cut(s) 174, 396
SspMI CTAG 1 cut(s) 212
StyD4I CCNGG 2 cut(s) 51, 347
StyI CCWWGG 1 cut(s) 586
TaaI ACNGT 5 cut(s) 442, 452, 460, 488, 505
TaiI ACGT 1 cut(s) 466
TaqI TCGA 4 cut(s) 69, 91, 194, 528
TasI AATT 2 cut(s) 190, 196
TfiI GAWTC 4 cut(s) 71, 108, 291, 491
TscAI CASTG 2 cut(s) 268, 520
TseFI GTSAC 3 cut(s) 436, 454, 515
TseI GCWGC 6 cut(s) 24, 171, 317, 383, 539, 542
Tsp45I GTSAC 3 cut(s) 436, 454, 515
TspDTI ATGAA 1 cut(s) 331
TspGWI ACGGA 2 cut(s) 48, 395
TspRI CASTG 2 cut(s) 268, 520
XbaI TCTAGA 1 cut(s) 211
XceI RCATGY 1 cut(s) 162
XhoI CTCGAG 1 cut(s) 90
XmiI GTMKAC 1 cut(s) 447
XspI CTAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.