Rmu_sc0009599.1_g000007

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009599.1
OG0015405
Physical Location & Seq
Forward (+)
20825 .. 23198
2374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009599.1_g000007.1.cds

Sequence Viewer

Length: 597 bp
atggctctgcagaccgcgaccgccggcaatgccctgctcccgaaatcggttctttctgtcaattcgccgaagatcgaggtcgggagagtggccggaggtgaaatctggagaagaaaccgaagagggttttgtgtgtgtaagagcagcgatgctgggaagaattgtggggtgcccaattcgaattacgtggtgcccttagacaaatcgttttcgtataactcgtcgtgtatcactcggcctctggctgagattctcagggacctcaacaagaggattcctgataacatcatcagtacacctcccagcactcgctccaccttcattccttggtaccatgcgaaccggatgctgagtttctatgctcctggatggtgtggagaaatccgtgatgtaatattttctgacaatggaagtgtgactgtggtataccgtgtcactgtacgaggatccgatgcagaggcacaccgtgagtcaactggaacagtttcacccagtgatggcaacattgttgatccagttgctgcagcagaggaaatagctttctgcagggcatgtgcccgatttggcctcggcctgtatctgtatcatgaggattag

Protein Analysis

198

Amino Acids

21.61

Weight (kDa)

8.49

Isoelectric Point (pI)

53.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47870
fragaria_vesca FvH4_4g21981
prunus_persica Prupe.1G209900_v2.0.a1 Prupe.1G209900_v2.0.a1 Prupe.1G209900_v2.0.a1
pyrus_communis pycom16g11550
rosa_chinensis RchiOBHm_Chr4g0428171
rosa_laevigata RLG00000007173
rosa_multiflora Rmu_sc0004940.1_g000007 Rmu_sc0009599.1_g000007
rosa_roxburghii Rroxscaffold_5G00369900
rosa_rugosa Rorug04G0223200
rosa_samantha Rh4AG280500 Rh4BG286000 Rh4DG283000
rosa_wichuraiana Rw4G024360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 330
AccB1I GGYRCC 3 cut(s) 169, 190, 330
AccI GTMKAC 1 cut(s) 426
AccII CGCG 1 cut(s) 17
AciI CCGC 2 cut(s) 15, 21
AclWI GGATC 3 cut(s) 441, 454, 506
AcoI YGGCCR 1 cut(s) 90
AdeI CACNNNGTG 2 cut(s) 467, 494
AfaI GTAC 3 cut(s) 295, 332, 441
AfiI CCNNNNNNNGG 2 cut(s) 46, 497
AjnI CCWGG 1 cut(s) 364
AluBI AGCT 1 cut(s) 539
AluI AGCT 1 cut(s) 539
AlwI GGATC 3 cut(s) 441, 454, 506
AlwNI CAGNNNCTG 1 cut(s) 521
AoxI GGCC 4 cut(s) 90, 236, 565, 571
ApeKI GCWGC 3 cut(s) 144, 521, 524
Asp700I GAANNNNTTC 1 cut(s) 484
Asp718I GGTACC 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 259
AsuHPI GGTGA 2 cut(s) 110, 480
AsuII TTCGAA 1 cut(s) 179
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 3 cut(s) 174, 195, 559
BamHI GGATCC 1 cut(s) 446
BanI GGYRCC 3 cut(s) 169, 190, 330
BbvI GCAGC 3 cut(s) 156, 508, 536
BccI CCATC 2 cut(s) 363, 491
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 1 cut(s) 366
BfmI CTRYAG 3 cut(s) 8, 522, 544
BisI GCNGC 3 cut(s) 145, 522, 525
BlsI GCNGC 3 cut(s) 146, 523, 526
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 5 cut(s) 171, 192, 260, 332, 448
BmrFI CCNGG 1 cut(s) 366
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 3 cut(s) 139, 336, 442
BmuI ACTGGG 1 cut(s) 486
BpmI CTGGAG 1 cut(s) 127
Bpu14I TTCGAA 1 cut(s) 179
BsaAI YACGTR 1 cut(s) 187
BsaJI CCNNGG 2 cut(s) 326, 568
BsaWI WCCGGW 1 cut(s) 342
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 497
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 3 cut(s) 481, 492, 515
Bse3DI GCAATG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 366
BseDI CCNNGG 2 cut(s) 326, 568
BseGI GGATG 2 cut(s) 351, 374
BseLI CCNNNNNNNGG 2 cut(s) 46, 497
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 3 cut(s) 237, 268, 341
BseNI ACTGG 3 cut(s) 481, 492, 515
BseSI GKGCMC 3 cut(s) 174, 195, 559
BseXI GCAGC 3 cut(s) 156, 508, 536
BseYI CCCAGC 2 cut(s) 152, 302
Bsh1236I CGCG 1 cut(s) 17
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 4 cut(s) 92, 238, 567, 573
BshNI GGYRCC 3 cut(s) 169, 190, 330
BsiEI CGRYCG 1 cut(s) 21
BsiSI CCGG 3 cut(s) 24, 93, 343
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 2 cut(s) 46, 497
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 4 cut(s) 92, 238, 567, 573
Bsp119I TTCGAA 1 cut(s) 179
Bsp1286I GDGCHC 3 cut(s) 174, 195, 559
Bsp143I GATC 3 cut(s) 72, 446, 511
BspACI CCGC 2 cut(s) 15, 21
BspANI GGCC 4 cut(s) 92, 238, 567, 573
BspCNI CTCAG 3 cut(s) 238, 267, 342
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 1 cut(s) 586
BspLI GGNNCC 5 cut(s) 171, 192, 260, 332, 448
BspMAI CTGCAG 3 cut(s) 12, 526, 548
BspPI GGATC 3 cut(s) 441, 454, 506
BspT104I TTCGAA 1 cut(s) 179
BspT107I GGYRCC 3 cut(s) 169, 190, 330
BsrDI GCAATG 1 cut(s) 34
BsrFI RCCGGY 1 cut(s) 23
BsrI ACTGG 3 cut(s) 481, 492, 515
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 2 cut(s) 326, 568
BssMI GATC 3 cut(s) 72, 446, 511
BssNAI GTATAC 1 cut(s) 427
BssT1I CCWWGG 1 cut(s) 326
Bst1107I GTATAC 1 cut(s) 427
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 5 cut(s) 421, 431, 439, 467, 484
Bst6I CTCTTC 1 cut(s) 115
BstBAI YACGTR 1 cut(s) 187
BstBI TTCGAA 1 cut(s) 179
BstC8I GCNNGC 1 cut(s) 25
BstDEI CTNAG 4 cut(s) 196, 246, 254, 350
BstF5I GGATG 2 cut(s) 351, 374
BstFNI CGCG 1 cut(s) 17
BstKTI GATC 3 cut(s) 75, 449, 514
BstMBI GATC 3 cut(s) 72, 446, 511
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 1 cut(s) 366
BstNSI RCATGY 1 cut(s) 555
BstSCI CCNGG 1 cut(s) 364
BstSFI CTRYAG 3 cut(s) 8, 522, 544
BstSLI GKGCMC 3 cut(s) 174, 195, 559
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 3 cut(s) 156, 508, 536
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
BstZ17I GTATAC 1 cut(s) 427
BsuRI GGCC 4 cut(s) 92, 238, 567, 573
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 2 cut(s) 351, 374
BtsIMutI CAGTG 2 cut(s) 435, 499
Cac8I GCNNGC 1 cut(s) 25
CaiI CAGNNNCTG 1 cut(s) 521
CciI TCATGA 1 cut(s) 586
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 259
Csp6I GTAC 3 cut(s) 294, 331, 440
CviAII CATG 3 cut(s) 335, 552, 587
CviJI RGCY 7 cut(s) 5, 92, 238, 245, 539, 567, 573
CviKI_1 RGCY 7 cut(s) 5, 92, 238, 245, 539, 567, 573
CviQI GTAC 3 cut(s) 294, 331, 440
DdeI CTNAG 4 cut(s) 196, 246, 254, 350
DpnI GATC 3 cut(s) 74, 448, 513
DpnII GATC 3 cut(s) 72, 446, 511
DraIII CACNNNGTG 2 cut(s) 467, 494
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 259
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 364
EcoT14I CCWWGG 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 326
FaeI CATG 3 cut(s) 338, 555, 590
FaiI YATR 6 cut(s) 216, 336, 360, 427, 553, 588
FaqI GGGAC 1 cut(s) 272
FatI CATG 3 cut(s) 334, 551, 586
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 3 cut(s) 145, 522, 525
FokI GGATG 2 cut(s) 358, 381
Fsp4HI GCNGC 3 cut(s) 145, 522, 525
GluI GCNGC 3 cut(s) 145, 522, 525
GsaI CCCAGC 2 cut(s) 156, 306
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 4 cut(s) 92, 238, 567, 573
HapII CCGG 3 cut(s) 24, 93, 343
Hin1II CATG 3 cut(s) 338, 555, 590
HincII GTYRAC 1 cut(s) 474
HindII GTYRAC 1 cut(s) 474
HinfI GANTC 3 cut(s) 250, 274, 470
HpaII CCGG 3 cut(s) 24, 93, 343
HphI GGTGA 2 cut(s) 110, 480
Hpy166II GTNNAC 3 cut(s) 296, 427, 474
Hpy188I TCNGA 2 cut(s) 403, 451
Hpy188III TCNNGA 5 cut(s) 40, 82, 106, 278, 587
Hpy8I GTNNAC 3 cut(s) 296, 427, 474
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 5 cut(s) 421, 431, 439, 467, 484
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 4 cut(s) 10, 455, 524, 546
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 4 cut(s) 196, 246, 254, 350
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 3 cut(s) 338, 555, 590
KpnI GGTACC 1 cut(s) 334
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Kzo9I GATC 3 cut(s) 72, 446, 511
LmnI GCTCC 3 cut(s) 42, 317, 367
Lsp1109I GCAGC 3 cut(s) 156, 508, 536
LweI GCATC 3 cut(s) 139, 336, 442
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 415, 433
MalI GATC 3 cut(s) 74, 448, 513
MboI GATC 3 cut(s) 72, 446, 511
MboII GAAGA 4 cut(s) 82, 123, 132, 169
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 3 cut(s) 174, 195, 559
MluCI AATT 4 cut(s) 61, 160, 175, 181
MlyI GAGTC 1 cut(s) 479
MroNI GCCGGC 1 cut(s) 23
MroXI GAANNNNTTC 1 cut(s) 484
MspI CCGG 3 cut(s) 24, 93, 343
MspR9I CCNGG 1 cut(s) 366
MvaI CCWGG 1 cut(s) 366
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 1 cut(s) 29
NaeI GCCGGC 1 cut(s) 25
NdeII GATC 3 cut(s) 72, 446, 511
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 3 cut(s) 338, 555, 590
NlaIV GGNNCC 5 cut(s) 171, 192, 260, 332, 448
NmeAIII GCCGAG 2 cut(s) 214, 549
NmuCI GTSAC 2 cut(s) 415, 433
NspI RCATGY 1 cut(s) 555
NspV TTCGAA 1 cut(s) 179
PagI TCATGA 1 cut(s) 586
PcsI WCGNNNNNNNCGW 1 cut(s) 218
PdiI GCCGGC 1 cut(s) 25
PdmI GAANNNNTTC 1 cut(s) 484
PfeI GAWTC 2 cut(s) 250, 274
PfoI TCCNGGA 1 cut(s) 364
PkrI GCNGC 3 cut(s) 146, 523, 526
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
Ppu21I YACGTR 1 cut(s) 187
PpuMI RGGWCCY 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 364
PspFI CCCAGC 2 cut(s) 152, 302
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 5 cut(s) 171, 192, 260, 332, 448
PspPI GGNCC 1 cut(s) 259
PspPPI RGGWCCY 1 cut(s) 259
PstI CTGCAG 3 cut(s) 12, 526, 548
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 1 cut(s) 446
RsaI GTAC 3 cut(s) 295, 332, 441
RsaNI GTAC 3 cut(s) 294, 331, 440
SatI GCNGC 3 cut(s) 145, 522, 525
Sau3AI GATC 3 cut(s) 72, 446, 511
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 3 cut(s) 174, 195, 559
SetI ASST 7 cut(s) 81, 100, 189, 264, 301, 320, 541
SfaNI GCATC 3 cut(s) 139, 336, 442
SfcI CTRYAG 3 cut(s) 8, 522, 544
SfuI TTCGAA 1 cut(s) 179
SinI GGWCC 1 cut(s) 259
Sse9I AATT 4 cut(s) 61, 160, 175, 181
SsiI CCGC 2 cut(s) 15, 21
SspI AATATT 1 cut(s) 396
StyD4I CCNGG 1 cut(s) 364
StyI CCWWGG 1 cut(s) 326
TaaI ACNGT 5 cut(s) 421, 431, 439, 467, 484
TaiI ACGT 1 cut(s) 189
TaqI TCGA 2 cut(s) 75, 179
TasI AATT 4 cut(s) 61, 160, 175, 181
TatI WGTACW 1 cut(s) 293
TfiI GAWTC 2 cut(s) 250, 274
TscAI CASTG 2 cut(s) 442, 499
TseFI GTSAC 2 cut(s) 415, 433
TseI GCWGC 3 cut(s) 144, 521, 524
Tsp45I GTSAC 2 cut(s) 415, 433
TspDTI ATGAA 1 cut(s) 310
TspGWI ACGGA 1 cut(s) 374
TspRI CASTG 2 cut(s) 442, 499
VpaK11BI GGWCC 1 cut(s) 259
XceI RCATGY 1 cut(s) 555
XmiI GTMKAC 1 cut(s) 426
XmnI GAANNNNTTC 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.