pycom16g12470

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008992
Physical Location & Seq
Reverse (-)
8740501 .. 8741686
1186 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
ATGGGATCCATCGCCCAAGAGAACACAAAATCTGTTGCCATACACAACTCAATGATTAGCAACGGTGATGACTGCATTTCGATAGGAACTGGGTGTGCAAATGTTGATATAATGGGTGTCACTTGTGGGCCAAGTCACGGGATTAGCATTGGGAGCCTTGGCGTGCACAACTCCCAGGCATGCGTCTCCAACATCACAGTCCGCAACTCCGTCATACGTGAATCCGACAACGGTGTCCGAATCAAGACATGGCAAGGCGGGACGGGCAGCGTCTCCGGCATCCTCTTCGAGAACATCCAGATGGAGAATGTGCTCAACTGCCTGCTAGTAGACCAGTACTACTGCCTATCAAAAGCATGCCGAAACGAAACCTCAGCAGTACTAGTCACAGACTTGACCTACAGAAACATAAAAGGCACGTACGATGTGAGGAGGCCTCCCATTCACTTTGCGTGCAGTGACACCGTGGCATGCAGAAATATTATCCTCTCAGAAGTTGAGCTTTACCCATACGAAGGAGAGCTCATGGATGAGCCCTTTTGTTGGAATGCTTATGGGACCCAGGAGACTGCAACTATTCCCCCTATTGATTGCTTGCGAGAGGGGGAGCCTCAGGCTTTGGATGAGGTGTCCAAGTATACTTGTTAG

Protein Analysis

216

Amino Acids

23.37

Weight (kDa)

4.69

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 7 - 168 4e-53 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AccI GTMKAC 2 cut(s) 330, 638
AciI CCGC 2 cut(s) 202, 258
AclWI GGATC 1 cut(s) 13
AfaI GTAC 3 cut(s) 338, 381, 422
AfiI CCNNNNNNNGG 3 cut(s) 137, 515, 543
AhlI ACTAGT 1 cut(s) 382
AjnI CCWGG 2 cut(s) 174, 561
AluBI AGCT 2 cut(s) 502, 523
AluI AGCT 2 cut(s) 502, 523
Alw21I GWGCWC 3 cut(s) 168, 315, 525
Alw26I GTCTC 3 cut(s) 190, 277, 560
Alw44I GTGCAC 1 cut(s) 164
AlwI GGATC 1 cut(s) 13
AoxI GGCC 2 cut(s) 128, 434
ApaLI GTGCAC 1 cut(s) 164
ApeKI GCWGC 1 cut(s) 267
AspS9I GGNCC 2 cut(s) 128, 558
AsuHPI GGTGA 1 cut(s) 77
AvaII GGWCC 1 cut(s) 558
AxyI CCTNAGG 1 cut(s) 612
BaeGI GKGCMC 1 cut(s) 168
BamHI GGATCC 1 cut(s) 5
BanII GRGCYC 2 cut(s) 525, 537
Bbv12I GWGCWC 3 cut(s) 168, 315, 525
BbvCI CCTCAGC 1 cut(s) 373
BbvI GCAGC 1 cut(s) 279
BccI CCATC 2 cut(s) 17, 295
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 2 cut(s) 176, 563
BcoDI GTCTC 3 cut(s) 190, 277, 560
BcuI ACTAGT 1 cut(s) 382
BfaI CTAG 2 cut(s) 326, 383
BfmI CTRYAG 1 cut(s) 400
BisI GCNGC 1 cut(s) 268
BlsI GCNGC 1 cut(s) 269
BmcAI AGTACT 2 cut(s) 338, 381
Bme1390I CCNGG 2 cut(s) 176, 563
Bme18I GGWCC 1 cut(s) 558
BmgT120I GGNCC 2 cut(s) 128, 558
BmiI GGNNCC 5 cut(s) 7, 155, 559, 560, 609
BmrFI CCNGG 2 cut(s) 176, 563
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 1 cut(s) 288
BmuI ACTGGG 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 421, 453
Bpu10I CCTNAGC 1 cut(s) 373
BsaAI YACGTR 2 cut(s) 218, 420
BsaJI CCNNGG 4 cut(s) 157, 174, 465, 561
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 515, 543
Bse1I ACTGG 2 cut(s) 94, 334
Bse21I CCTNAGG 1 cut(s) 612
BseBI CCWGG 2 cut(s) 176, 563
BseDI CCNNGG 4 cut(s) 157, 174, 465, 561
BseGI GGATG 4 cut(s) 279, 294, 535, 628
BseLI CCNNNNNNNGG 3 cut(s) 137, 515, 543
BseMII CTCAG 3 cut(s) 387, 504, 626
BseNI ACTGG 2 cut(s) 94, 334
BseRI GAGGAG 1 cut(s) 445
BseSI GKGCMC 1 cut(s) 168
BseXI GCAGC 1 cut(s) 279
BsgI GTGCAG 1 cut(s) 475
BshFI GGCC 2 cut(s) 130, 436
BsiHKAI GWGCWC 3 cut(s) 168, 315, 525
BsiSI CCGG 1 cut(s) 276
BsiWI CGTACG 1 cut(s) 420
BslFI GGGAC 2 cut(s) 274, 571
BslI CCNNNNNNNGG 3 cut(s) 137, 515, 543
BsmAI GTCTC 3 cut(s) 190, 277, 560
BsmBI CGTCTC 2 cut(s) 190, 277
BsmFI GGGAC 2 cut(s) 274, 571
BsmI GAATGC 1 cut(s) 553
BsnI GGCC 2 cut(s) 130, 436
Bsp1286I GDGCHC 4 cut(s) 168, 315, 525, 537
Bsp143I GATC 1 cut(s) 5
BspACI CCGC 2 cut(s) 202, 258
BspANI GGCC 2 cut(s) 130, 436
BspCNI CTCAG 3 cut(s) 386, 503, 625
BspLI GGNNCC 5 cut(s) 7, 155, 559, 560, 609
BspPI GGATC 1 cut(s) 13
BsrI ACTGG 2 cut(s) 94, 334
BssECI CCNNGG 4 cut(s) 157, 174, 465, 561
BssMI GATC 1 cut(s) 5
BssNAI GTATAC 1 cut(s) 639
BssT1I CCWWGG 1 cut(s) 157
Bst1107I GTATAC 1 cut(s) 639
Bst2UI CCWGG 2 cut(s) 176, 563
Bst4CI ACNGT 4 cut(s) 65, 199, 233, 466
Bst6I CTCTTC 1 cut(s) 290
BstBAI YACGTR 2 cut(s) 218, 420
BstC8I GCNNGC 7 cut(s) 164, 181, 323, 358, 454, 472, 596
BstDEI CTNAG 3 cut(s) 373, 490, 612
BstDSI CCRYGG 1 cut(s) 465
BstF5I GGATG 4 cut(s) 279, 294, 535, 628
BstKTI GATC 1 cut(s) 8
BstMAI GTCTC 3 cut(s) 190, 277, 560
BstMBI GATC 1 cut(s) 5
BstMWI GCNNNNNNNGC 3 cut(s) 153, 264, 276
BstNI CCWGG 2 cut(s) 176, 563
BstNSI RCATGY 3 cut(s) 183, 360, 474
BstSCI CCNGG 2 cut(s) 174, 561
BstSFI CTRYAG 1 cut(s) 400
BstSLI GKGCMC 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 279
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
BstZ17I GTATAC 1 cut(s) 639
Bsu36I CCTNAGG 1 cut(s) 612
BsuRI GGCC 2 cut(s) 130, 436
BtgI CCRYGG 1 cut(s) 465
BtsCI GGATG 4 cut(s) 279, 294, 535, 628
BtsI GCAGTG 1 cut(s) 463
BtsIMutI CAGTG 1 cut(s) 463
Cac8I GCNNGC 7 cut(s) 164, 181, 323, 358, 454, 472, 596
Cfr13I GGNCC 2 cut(s) 128, 558
CseI GACGC 2 cut(s) 172, 259
Csp6I GTAC 3 cut(s) 337, 380, 421
CviAII CATG 5 cut(s) 180, 249, 357, 471, 526
CviJI RGCY 8 cut(s) 130, 156, 436, 502, 523, 535, 610, 617
CviKI_1 RGCY 8 cut(s) 130, 156, 436, 502, 523, 535, 610, 617
CviQI GTAC 3 cut(s) 337, 380, 421
DdeI CTNAG 3 cut(s) 373, 490, 612
DpnI GATC 1 cut(s) 7
DpnII GATC 1 cut(s) 5
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Ecl136II GAGCTC 1 cut(s) 523
Eco130I CCWWGG 1 cut(s) 157
Eco147I AGGCCT 1 cut(s) 436
Eco24I GRGCYC 2 cut(s) 525, 537
Eco47I GGWCC 1 cut(s) 558
Eco53kI GAGCTC 1 cut(s) 523
Eco81I CCTNAGG 1 cut(s) 612
EcoICRI GAGCTC 1 cut(s) 523
EcoO109I RGGNCCY 1 cut(s) 558
EcoRII CCWGG 2 cut(s) 174, 561
EcoT14I CCWWGG 1 cut(s) 157
EcoT38I GRGCYC 2 cut(s) 525, 537
ErhI CCWWGG 1 cut(s) 157
Esp3I CGTCTC 2 cut(s) 190, 277
FaeI CATG 5 cut(s) 183, 252, 360, 474, 529
FalI AAGNNNNNCTT 2 cut(s) 486, 518
FaqI GGGAC 2 cut(s) 274, 571
FatI CATG 5 cut(s) 179, 248, 356, 470, 525
FauI CCCGC 1 cut(s) 251
FblI GTMKAC 2 cut(s) 330, 638
Fnu4HI GCNGC 1 cut(s) 268
FokI GGATG 4 cut(s) 266, 281, 542, 635
FriOI GRGCYC 2 cut(s) 525, 537
Fsp4HI GCNGC 1 cut(s) 268
FspBI CTAG 2 cut(s) 326, 383
GluI GCNGC 1 cut(s) 268
HaeIII GGCC 2 cut(s) 130, 436
HapII CCGG 1 cut(s) 276
HgaI GACGC 2 cut(s) 172, 259
Hin1II CATG 5 cut(s) 183, 252, 360, 474, 529
HinfI GANTC 2 cut(s) 221, 240
HpaII CCGG 1 cut(s) 276
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 3 cut(s) 166, 331, 639
Hpy188I TCNGA 3 cut(s) 226, 239, 493
Hpy188III TCNNGA 3 cut(s) 244, 289, 298
Hpy8I GTNNAC 3 cut(s) 166, 331, 639
HpyAV CCTTC 1 cut(s) 509
HpyCH4III ACNGT 4 cut(s) 65, 199, 233, 466
HpyCH4IV ACGT 2 cut(s) 217, 419
HpyCH4V TGCA 6 cut(s) 75, 98, 166, 456, 474, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 264, 276
HpyF3I CTNAG 3 cut(s) 373, 490, 612
HpySE526I ACGT 2 cut(s) 217, 419
Hsp92II CATG 5 cut(s) 183, 252, 360, 474, 529
KflI GGGWCCC 1 cut(s) 558
Kzo9I GATC 1 cut(s) 5
LmnI GCTCC 2 cut(s) 153, 607
Lsp1109I GCAGC 1 cut(s) 279
LweI GCATC 1 cut(s) 288
MaeI CTAG 2 cut(s) 326, 383
MaeII ACGT 2 cut(s) 217, 419
MaeIII GTNAC 4 cut(s) 118, 134, 385, 458
MalI GATC 1 cut(s) 7
MboI GATC 1 cut(s) 5
MboII GAAGA 1 cut(s) 277
MflI RGATCY 1 cut(s) 5
MhlI GDGCHC 4 cut(s) 168, 315, 525, 537
MmeI TCCRAC 3 cut(s) 213, 249, 524
MnlI CCTC 9 cut(s) 293, 382, 423, 426, 447, 497, 595, 619, 621
MslI CAYNNNNRTG 1 cut(s) 299
MspI CCGG 1 cut(s) 276
MspR9I CCNGG 2 cut(s) 176, 563
Mva1269I GAATGC 1 cut(s) 553
MvaI CCWGG 2 cut(s) 176, 563
MwoI GCNNNNNNNGC 3 cut(s) 153, 264, 276
NdeII GATC 1 cut(s) 5
NlaIII CATG 5 cut(s) 183, 252, 360, 474, 529
NlaIV GGNNCC 5 cut(s) 7, 155, 559, 560, 609
NmuCI GTSAC 4 cut(s) 118, 134, 385, 458
NspI RCATGY 3 cut(s) 183, 360, 474
PaeI GCATGC 3 cut(s) 183, 360, 474
PceI AGGCCT 1 cut(s) 436
PctI GAATGC 1 cut(s) 553
PfeI GAWTC 2 cut(s) 221, 240
Pfl23II CGTACG 1 cut(s) 420
PkrI GCNGC 1 cut(s) 269
Ppu21I YACGTR 2 cut(s) 218, 420
PpuMI RGGWCCY 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 525
Psp5II RGGWCCY 1 cut(s) 558
Psp6I CCWGG 2 cut(s) 174, 561
PspGI CCWGG 2 cut(s) 174, 561
PspLI CGTACG 1 cut(s) 420
PspN4I GGNNCC 5 cut(s) 7, 155, 559, 560, 609
PspPI GGNCC 2 cut(s) 128, 558
PspPPI RGGWCCY 1 cut(s) 558
PsuI RGATCY 1 cut(s) 5
RsaI GTAC 3 cut(s) 338, 381, 422
RsaNI GTAC 3 cut(s) 337, 380, 421
RseI CAYNNNNRTG 1 cut(s) 299
SacI GAGCTC 1 cut(s) 525
SatI GCNGC 1 cut(s) 268
Sau3AI GATC 1 cut(s) 5
Sau96I GGNCC 2 cut(s) 128, 558
ScaI AGTACT 2 cut(s) 338, 381
ScrFI CCNGG 2 cut(s) 176, 563
SduI GDGCHC 4 cut(s) 168, 315, 525, 537
SetI ASST 7 cut(s) 220, 374, 401, 422, 504, 525, 630
SfaNI GCATC 1 cut(s) 288
SfcI CTRYAG 1 cut(s) 400
SinI GGWCC 1 cut(s) 558
SmiMI CAYNNNNRTG 1 cut(s) 299
SpeI ACTAGT 1 cut(s) 382
SphI GCATGC 3 cut(s) 183, 360, 474
SseBI AGGCCT 1 cut(s) 436
SsiI CCGC 2 cut(s) 202, 258
SspI AATATT 1 cut(s) 481
SspMI CTAG 2 cut(s) 326, 383
SstI GAGCTC 1 cut(s) 525
StuI AGGCCT 1 cut(s) 436
StyD4I CCNGG 2 cut(s) 174, 561
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 4 cut(s) 65, 199, 233, 466
TaiI ACGT 2 cut(s) 220, 422
TaqI TCGA 2 cut(s) 80, 288
TatI WGTACW 2 cut(s) 336, 379
TfiI GAWTC 2 cut(s) 221, 240
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 4 cut(s) 118, 134, 385, 458
TseI GCWGC 1 cut(s) 267
Tsp45I GTSAC 4 cut(s) 118, 134, 385, 458
TspGWI ACGGA 1 cut(s) 199
TspRI CASTG 1 cut(s) 463
VneI GTGCAC 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 558
XceI RCATGY 3 cut(s) 183, 360, 474
XmiI GTMKAC 2 cut(s) 330, 638
XspI CTAG 2 cut(s) 326, 383
ZrmI AGTACT 2 cut(s) 338, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.