pycom16g13130

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0016309
Physical Location & Seq
Forward (+)
9356547 .. 9357914
1368 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1368 bp
ATGGAAAATAGTTACAGATCAGAGAAACAGGAACAAGCAAGTGACCATGTCGCCCCCGAAATTATTCCTGAGCCACAAACAAGAGACCATGTCGCCCTCGAAATCATTCCTGAGCATGATGTCCTTGTATCTTCCATCAAAGAAAAGATGGAAAACGTTGCTGTATCAGTCTGCATTTTCCAAGTCCCCGACAAACTTCTCGATGGCAACAAAAAGAAGTATACTCCAGAGAATGTTTCCATCGGTCCTTTGCACCGCAGAGAACCTACTAAAGTCGGGGAAGATGATAAGTGGCGGTATTCTTATGCACTCCTCAATCGAAAACCAAATTTAGAAGCAAGCTTGAGCATCTGTGTGAAAGCTCTTAGAGAGTTAGAGCACAAAGCGAGAAGATGCTACGAGGGAGAGATTGGCCTCTCTAGTGATGAATTTGTGCAGTTGTTGCTAGTTGATGGCTGCTTCATCATTGAGCTGTTTCTTAAGTACGCTTACAAGAGCCTCAGATCGCGACGAGATCCTATATTCAGCACGGTAGGGATGCTTTTCGGCATGAGGAATGACTTGGCATTGTTACTAGAAAACCAAATTCCTTTCTTTGTTGTTCAAAGGTTGTTTCAAGTAGTACCGCTTCCTAAAAAATGTAATGAGTCACTCAGTGAGCTCGCTGCACGCTTCTGCAATAGAATAATCCCGGGAGATGATGAGCATGTTATCCAAGACAAGTTCAACCGAGAAGGGTATCATTTGCTCGATCTGATTCGACATTCCATCCTCCCAACATATCCAAAACTGCCACCGAAAGAAGATACACCTCCCAAAAATATGGATTGTGCAAAAATTCTCAAAAGGGCAGGCATTAAGTTCAAGAGCGCTAGAAGCCCAAGTTTCTTGGACATCAAGTTTTCTCGCGGAGTTTTCAAAATCCCCTCCCTTGAAGTCCACGGATACACAGAAACACTTCTCCGGAACCTCGTTGCACTGGAGCAGCGTCAGATTGACGATACAGTACAGCACGTCACATCCTACGCCTTCCTCATGGGATGCCTCATCAGCTCTGAGAAAGATGTGAAGTTCCTCCGGCGAAGGGAGATTCTCACGCACGACCAGAAGAAGGATAATGAGGTTTTCGAAGTGTTTAAGAATCTATGCAAGGAGATCAGCTTGAAGGATTTTTACTACGTGAGGATTTTTGAAGAGGTAGGTGAGTACAAGAGAAAGAGTTGGCATGAAAAGCGGCAGAAGTTGAAGCACAAGCATCTCAAAACTTCTACCTCAGTTGTTGTGTTTGTTGTTGCAATTTTGCTTCTTCTTCTCACCTTTGTTGGAGCTTGCTTTTCCATTCTTACCTTTGCTCTCCACCATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

456

Amino Acids

52.72

Weight (kDa)

8.8

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 59 - 438 1.1e-107 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19590
malus_domestica MD16G1154900.v1.1
prunus_persica Prupe.1G118600_v2.0.a1
pyrus_communis pycom16g13130
rosa_chinensis RchiOBHm_Chr4g0424851
rosa_laevigata RLG00000007415
rosa_multiflora Rmu_sc0006754.1_g000008
rosa_roxburghii Rroxscaffold_5G00367070
rosa_rugosa Rorug04G0199600 Rorug04G0199700
rosa_samantha Rh4AG257400 Rh4BG262800 Rh4CG273700 Rh4DG258500
rosa_wichuraiana Rw4G022210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 221
AccII CGCG 2 cut(s) 508, 909
AccIII TCCGGA 1 cut(s) 963
AciI CCGC 5 cut(s) 256, 295, 626, 909, 1234
AclI AACGTT 1 cut(s) 156
AclWI GGATC 1 cut(s) 509
AcsI RAATTY 4 cut(s) 328, 428, 585, 837
AdeI CACNNNGTG 1 cut(s) 656
AfaI GTAC 4 cut(s) 485, 624, 1008, 1208
AfeI AGCGCT 1 cut(s) 871
AfiI CCNNNNNNNGG 2 cut(s) 1084, 1111
AflII CTTAAG 1 cut(s) 479
AgsI TTSAA 9 cut(s) 605, 617, 727, 865, 919, 935, 1165, 1193, 1246
AjiI CACGTC 1 cut(s) 1015
AluBI AGCT 7 cut(s) 342, 362, 472, 661, 1053, 1161, 1328
AluI AGCT 7 cut(s) 342, 362, 472, 661, 1053, 1161, 1328
Alw21I GWGCWC 2 cut(s) 381, 663
Alw26I GTCTC 1 cut(s) 78
AlwI GGATC 1 cut(s) 509
Ama87I CYCGRG 1 cut(s) 691
Aor13HI TCCGGA 1 cut(s) 963
Aor51HI AGCGCT 1 cut(s) 871
AoxI GGCC 1 cut(s) 412
ApeKI GCWGC 3 cut(s) 456, 665, 985
ApoI RAATTY 4 cut(s) 328, 428, 585, 837
Asp700I GAANNNNTTC 3 cut(s) 63, 105, 957
AspLEI GCGC 1 cut(s) 872
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 2 cut(s) 692, 693
AsuHPI GGTGA 2 cut(s) 1214, 1306
AsuII TTCGAA 1 cut(s) 1128
AvaI CYCGRG 1 cut(s) 691
AvaII GGWCC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 663
BarI GAAGNNNNNNTAC 4 cut(s) 612, 644, 1157, 1189
Bbv12I GWGCWC 2 cut(s) 381, 663
BbvI GCAGC 3 cut(s) 443, 652, 997
BccI CCATC 6 cut(s) 142, 143, 197, 248, 446, 776
BciVI GTATCC 1 cut(s) 938
BcnI CCSGG 2 cut(s) 692, 693
BcoDI GTCTC 1 cut(s) 78
BfaI CTAG 4 cut(s) 420, 446, 575, 873
BfoI RGCGCY 1 cut(s) 873
BfrI CTTAAG 1 cut(s) 479
BfuI GTATCC 1 cut(s) 938
BisI GCNGC 4 cut(s) 457, 666, 986, 1235
BlsI GCNGC 4 cut(s) 458, 667, 987, 1236
Bme1390I CCNGG 2 cut(s) 692, 693
Bme18I GGWCC 1 cut(s) 245
BmeT110I CYCGRG 1 cut(s) 691
BmgBI CACGTC 1 cut(s) 1015
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 1 cut(s) 968
BmrFI CCNGG 2 cut(s) 692, 693
BmsI GCATC 5 cut(s) 357, 383, 528, 1031, 1264
BpmI CTGGAG 2 cut(s) 210, 1001
Bpu10I CCTNAGC 2 cut(s) 69, 111
Bpu14I TTCGAA 1 cut(s) 1128
BpuEI CTTGAG 1 cut(s) 364
BpuMI CCSGG 2 cut(s) 692, 693
BsaAI YACGTR 1 cut(s) 1180
BsaI GGTCTC 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 691, 940
BsaWI WCCGGW 1 cut(s) 963
Bsc4I CCNNNNNNNGG 2 cut(s) 1084, 1111
Bse1I ACTGG 1 cut(s) 984
BseAI TCCGGA 1 cut(s) 963
BseDI CCNNGG 2 cut(s) 691, 940
BseGI GGATG 4 cut(s) 543, 768, 1019, 1046
BseLI CCNNNNNNNGG 2 cut(s) 1084, 1111
BseMII CTCAG 6 cut(s) 60, 102, 514, 667, 1047, 1287
BseNI ACTGG 1 cut(s) 984
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 3 cut(s) 443, 652, 997
BsgI GTGCAG 2 cut(s) 455, 651
Bsh1236I CGCG 2 cut(s) 508, 909
BshFI GGCC 1 cut(s) 414
BsiHKAI GWGCWC 2 cut(s) 381, 663
BsiHKCI CYCGRG 1 cut(s) 691
BsiSI CCGG 3 cut(s) 692, 964, 1078
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 1084, 1111
BsmAI GTCTC 1 cut(s) 78
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 1 cut(s) 414
Bso31I GGTCTC 1 cut(s) 78
BsoBI CYCGRG 1 cut(s) 691
Bsp119I TTCGAA 1 cut(s) 1128
Bsp1286I GDGCHC 2 cut(s) 381, 663
Bsp13I TCCGGA 1 cut(s) 963
Bsp143I GATC 5 cut(s) 17, 503, 514, 751, 1155
Bsp68I TCGCGA 1 cut(s) 508
BspACI CCGC 5 cut(s) 256, 295, 626, 909, 1234
BspANI GGCC 1 cut(s) 414
BspCNI CTCAG 6 cut(s) 61, 103, 513, 666, 1048, 1286
BspEI TCCGGA 1 cut(s) 963
BspFNI CGCG 2 cut(s) 508, 909
BspLI GGNNCC 1 cut(s) 968
BspPI GGATC 1 cut(s) 509
BspT104I TTCGAA 1 cut(s) 1128
BspTI CTTAAG 1 cut(s) 479
BspTNI GGTCTC 1 cut(s) 78
BsrI ACTGG 1 cut(s) 984
BssECI CCNNGG 2 cut(s) 691, 940
BssMI GATC 5 cut(s) 17, 503, 514, 751, 1155
BssNAI GTATAC 1 cut(s) 222
Bst1107I GTATAC 1 cut(s) 222
Bst4CI ACNGT 2 cut(s) 532, 1006
Bst6I CTCTTC 1 cut(s) 1188
BstAFI CTTAAG 1 cut(s) 479
BstAPI GCANNNNNTGC 1 cut(s) 442
BstBAI YACGTR 1 cut(s) 1180
BstBI TTCGAA 1 cut(s) 1128
BstC8I GCNNGC 5 cut(s) 340, 663, 670, 853, 1330
BstDEI CTNAG 7 cut(s) 69, 111, 365, 500, 653, 1056, 1273
BstDSI CCRYGG 1 cut(s) 940
BstF5I GGATG 4 cut(s) 543, 768, 1019, 1046
BstFNI CGCG 2 cut(s) 508, 909
BstH2I RGCGCY 1 cut(s) 873
BstHHI GCGC 1 cut(s) 872
BstKTI GATC 5 cut(s) 20, 506, 517, 754, 1158
BstMAI GTCTC 1 cut(s) 78
BstMBI GATC 5 cut(s) 17, 503, 514, 751, 1155
BstMWI GCNNNNNNNGC 4 cut(s) 442, 876, 1050, 1231
BstNSI RCATGY 1 cut(s) 710
BstSCI CCNGG 2 cut(s) 690, 691
BstUI CGCG 2 cut(s) 508, 909
BstV1I GCAGC 3 cut(s) 443, 652, 997
BstX2I RGATCY 1 cut(s) 514
BstXI CCANNNNNNTGG 1 cut(s) 823
BstYI RGATCY 1 cut(s) 514
BstZ17I GTATAC 1 cut(s) 222
BsuI GTATCC 1 cut(s) 938
BsuRI GGCC 1 cut(s) 414
BtgI CCRYGG 1 cut(s) 940
BtrI CACGTC 1 cut(s) 1015
BtsCI GGATG 4 cut(s) 543, 768, 1019, 1046
BtsIMutI CAGTG 2 cut(s) 661, 977
BtuMI TCGCGA 1 cut(s) 508
Cac8I GCNNGC 5 cut(s) 340, 663, 670, 853, 1330
CfoI GCGC 1 cut(s) 872
Cfr13I GGNCC 1 cut(s) 245
Cfr9I CCCGGG 1 cut(s) 691
CseI GACGC 1 cut(s) 977
Csp6I GTAC 4 cut(s) 484, 623, 1007, 1207
CviAII CATG 8 cut(s) 47, 89, 116, 550, 707, 1036, 1226, 1361
CviQI GTAC 4 cut(s) 484, 623, 1007, 1207
DdeI CTNAG 7 cut(s) 69, 111, 365, 500, 653, 1056, 1273
DpnI GATC 5 cut(s) 19, 505, 516, 753, 1157
DpnII GATC 5 cut(s) 17, 503, 514, 751, 1155
DraIII CACNNNGTG 1 cut(s) 656
Eam1104I CTCTTC 1 cut(s) 1188
EarI CTCTTC 1 cut(s) 1188
Ecl136II GAGCTC 1 cut(s) 661
Eco24I GRGCYC 1 cut(s) 663
Eco31I GGTCTC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 245
Eco47III AGCGCT 1 cut(s) 871
Eco53kI GAGCTC 1 cut(s) 661
Eco88I CYCGRG 1 cut(s) 691
EcoICRI GAGCTC 1 cut(s) 661
EcoT38I GRGCYC 1 cut(s) 663
FaeI CATG 8 cut(s) 50, 92, 119, 553, 710, 1039, 1229, 1364
FaqI GGGAC 1 cut(s) 170
FatI CATG 8 cut(s) 46, 88, 115, 549, 706, 1035, 1225, 1360
FblI GTMKAC 1 cut(s) 221
Fnu4HI GCNGC 4 cut(s) 457, 666, 986, 1235
FokI GGATG 4 cut(s) 550, 755, 1006, 1053
FriOI GRGCYC 1 cut(s) 663
Fsp4HI GCNGC 4 cut(s) 457, 666, 986, 1235
FspBI CTAG 4 cut(s) 420, 446, 575, 873
GlaI GCGC 1 cut(s) 871
GluI GCNGC 4 cut(s) 457, 666, 986, 1235
GsuI CTGGAG 2 cut(s) 210, 1001
HaeII RGCGCY 1 cut(s) 873
HaeIII GGCC 1 cut(s) 414
HapII CCGG 3 cut(s) 692, 964, 1078
HgaI GACGC 1 cut(s) 977
HhaI GCGC 1 cut(s) 872
Hin1II CATG 8 cut(s) 50, 92, 119, 553, 710, 1039, 1229, 1364
Hin6I GCGC 1 cut(s) 870
HinP1I GCGC 1 cut(s) 870
HindIII AAGCTT 1 cut(s) 340
HinfI GANTC 4 cut(s) 647, 757, 1090, 1141
HpaII CCGG 3 cut(s) 692, 964, 1078
HphI GGTGA 2 cut(s) 1214, 1306
Hpy166II GTNNAC 2 cut(s) 222, 940
Hpy188I TCNGA 5 cut(s) 22, 503, 756, 993, 1057
Hpy188III TCNNGA 7 cut(s) 68, 110, 200, 227, 507, 865, 964
Hpy8I GTNNAC 2 cut(s) 222, 940
Hpy99I CGWCG 1 cut(s) 513
HpyAV CCTTC 5 cut(s) 728, 1039, 1077, 1105, 1159
HpyCH4III ACNGT 2 cut(s) 532, 1006
HpyCH4IV ACGT 3 cut(s) 156, 1014, 1179
HpyF10VI GCNNNNNNNGC 4 cut(s) 442, 876, 1050, 1231
HpyF3I CTNAG 7 cut(s) 69, 111, 365, 500, 653, 1056, 1273
HpySE526I ACGT 3 cut(s) 156, 1014, 1179
Hsp92II CATG 8 cut(s) 50, 92, 119, 553, 710, 1039, 1229, 1364
HspAI GCGC 1 cut(s) 870
Kpn2I TCCGGA 1 cut(s) 963
Kzo9I GATC 5 cut(s) 17, 503, 514, 751, 1155
LmnI GCTCC 2 cut(s) 982, 1325
Lsp1109I GCAGC 3 cut(s) 443, 652, 997
LweI GCATC 5 cut(s) 357, 383, 528, 1031, 1264
MaeI CTAG 4 cut(s) 420, 446, 575, 873
MaeII ACGT 3 cut(s) 156, 1014, 1179
MaeIII GTNAC 5 cut(s) 11, 41, 570, 648, 1015
MalI GATC 5 cut(s) 19, 505, 516, 753, 1157
MboI GATC 5 cut(s) 17, 503, 514, 751, 1155
MboII GAAGA 8 cut(s) 123, 293, 402, 815, 1120, 1205, 1298, 1301
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 2 cut(s) 381, 663
MluCI AATT 6 cut(s) 60, 328, 428, 585, 837, 1296
MlyI GAGTC 1 cut(s) 656
MmeI TCCRAC 1 cut(s) 1303
MroI TCCGGA 1 cut(s) 963
MroXI GAANNNNTTC 3 cut(s) 63, 105, 957
MseI TTAA 3 cut(s) 480, 858, 1137
MslI CAYNNNNRTG 1 cut(s) 353
MspCI CTTAAG 1 cut(s) 479
MspI CCGG 3 cut(s) 692, 964, 1078
MspR9I CCNGG 2 cut(s) 692, 693
MvnI CGCG 2 cut(s) 508, 909
MwoI GCNNNNNNNGC 4 cut(s) 442, 876, 1050, 1231
NciI CCSGG 2 cut(s) 692, 693
NdeII GATC 5 cut(s) 17, 503, 514, 751, 1155
NlaIII CATG 8 cut(s) 50, 92, 119, 553, 710, 1039, 1229, 1364
NlaIV GGNNCC 1 cut(s) 968
NmuCI GTSAC 3 cut(s) 41, 648, 1015
NruI TCGCGA 1 cut(s) 508
NspI RCATGY 1 cut(s) 710
NspV TTCGAA 1 cut(s) 1128
PdmI GAANNNNTTC 3 cut(s) 63, 105, 957
PfeI GAWTC 3 cut(s) 757, 1090, 1141
PflFI GACNNNGTC 2 cut(s) 47, 89
PkrI GCNGC 4 cut(s) 458, 667, 987, 1236
PleI GAGTC 1 cut(s) 655
PpsI GAGTC 1 cut(s) 655
Ppu21I YACGTR 1 cut(s) 1180
Psp124BI GAGCTC 1 cut(s) 663
Psp1406I AACGTT 1 cut(s) 156
PspN4I GGNNCC 1 cut(s) 968
PspPI GGNCC 1 cut(s) 245
PsuI RGATCY 1 cut(s) 514
PsyI GACNNNGTC 2 cut(s) 47, 89
RruI TCGCGA 1 cut(s) 508
RsaI GTAC 4 cut(s) 485, 624, 1008, 1208
RsaNI GTAC 4 cut(s) 484, 623, 1007, 1207
RseI CAYNNNNRTG 1 cut(s) 353
SacI GAGCTC 1 cut(s) 663
SaqAI TTAA 3 cut(s) 480, 858, 1137
SatI GCNGC 4 cut(s) 457, 666, 986, 1235
Sau3AI GATC 5 cut(s) 17, 503, 514, 751, 1155
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 1 cut(s) 656
ScrFI CCNGG 2 cut(s) 692, 693
SduI GDGCHC 2 cut(s) 381, 663
SfaNI GCATC 5 cut(s) 357, 383, 528, 1031, 1264
SfuI TTCGAA 1 cut(s) 1128
SinI GGWCC 1 cut(s) 245
SmaI CCCGGG 1 cut(s) 693
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 2 cut(s) 343, 479
SmoI CTYRAG 2 cut(s) 343, 479
Sse9I AATT 6 cut(s) 60, 328, 428, 585, 837, 1296
SsiI CCGC 5 cut(s) 256, 295, 626, 909, 1234
SspMI CTAG 4 cut(s) 420, 446, 575, 873
SstI GAGCTC 1 cut(s) 663
StyD4I CCNGG 2 cut(s) 690, 691
TaaI ACNGT 2 cut(s) 532, 1006
TaiI ACGT 3 cut(s) 159, 1017, 1182
TaqI TCGA 6 cut(s) 99, 201, 319, 750, 760, 1128
TaqII GACCGA 1 cut(s) 233
TasI AATT 6 cut(s) 60, 328, 428, 585, 837, 1296
TatI WGTACW 2 cut(s) 1006, 1206
TauI GCSGC 1 cut(s) 1237
TfiI GAWTC 3 cut(s) 757, 1090, 1141
Tru1I TTAA 3 cut(s) 480, 858, 1137
Tru9I TTAA 3 cut(s) 480, 858, 1137
TscAI CASTG 2 cut(s) 661, 984
TseFI GTSAC 3 cut(s) 41, 648, 1015
TseI GCWGC 3 cut(s) 456, 665, 985
Tsp45I GTSAC 3 cut(s) 41, 648, 1015
TspDTI ATGAA 3 cut(s) 441, 451, 1242
TspGWI ACGGA 1 cut(s) 957
TspMI CCCGGG 1 cut(s) 691
TspRI CASTG 2 cut(s) 661, 984
Tth111I GACNNNGTC 2 cut(s) 47, 89
Vha464I CTTAAG 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 245
XapI RAATTY 4 cut(s) 328, 428, 585, 837
XceI RCATGY 1 cut(s) 710
XmaI CCCGGG 1 cut(s) 691
XmiI GTMKAC 1 cut(s) 221
XmnI GAANNNNTTC 3 cut(s) 63, 105, 957
XspI CTAG 4 cut(s) 420, 446, 575, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.