Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0007758

Physical Location & Seq

Genomic Coordinates Reverse (-)

10579491 .. 10584181

Gene Structure 4691 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g14500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1965 bp

ATGGCGACCATATCCATAAGCTTCTGCCCGTTCGCCATGGCTCGCCCGAGGGTCCCACAGACTCGCAAGCCTCCGAATAAGAAGCAGGTAACTCCGAGGCCTAACCCTAACCCTAACGTTAGGCTGAAGGGTCGAACCGGCGCGAAGCAGTTGTCCTCCGTTGGCGTCGAGGCCACCACCTACACTCGCCTGCCTCCCAGAGAGGATTTCTCTCTCCCGTCTCTCGATGCGTCGTTGTTGGAGCTCTCGTCTGAGGTGAAGCTCTCTGAGTCAAATGCCGCATTGGTGGTTGAGAAGGAGATTGATTATTTAAGCAGCGACGAAGAGGAGGAGGAAGAAAGTGGCACATATAAGAAATTAGGGATGAATTCTGGGAAATTTGAGGTCTTTGATGGGAATTACGATTCTGAACTGGATGAGGAGGAGGAGGAGGAGGATGACAGTGATGGTGAAATTGACGGCGAACTGGAAAATGTTTATCAAAACGACGACGGTGAGTTCCTAGGGTTCAAGGAGGGCAAAGCTGTGAATTTTTCCGGTAATGAGGGTGAATTGGAGGAAGTGGAAGTGAAGGAGAAGGGAGTGCCAGCGGTAATGCGGTGTTTCGACCGCGCCAAAATCTTTGCCAAGGCAGGGGACGGAGGAAACGGCGTGGTTGCAATGCGGCGGGAAAAGTTCGTGCCTTTGGGGGGACCTTCAGGCGGCGACGGAGGGCGAGGTGGGAATGTGTATATGGAGGTGGATGGGTCGATGAATTCACTTCTGCCGTTTCGAAACAGCGTACATTTTCGGGCAGGGAGAGGTGATCATGGAAGAGGGCAGTCTCAGAATGGGGCTAAGGGAGAGGACGTAGTGATTAAGGTGGCGCCCGGGACTGTTGTCCGAGAGGCGGGGAAGGATGAGGTGCTTCTGGAGTTGTTGCATCCGGGGCAGCGTGCATTGTTGTTGCCTGGAGGGAGAGGCGGGAGAGGAAACGCTTCGTTTAAATCCGGGACAAATAAGGTCCCCAGGATTGCTGAGAATGGTGCAGAGGGTCCCGAGATGTGGTTGGAGCTGGAACTAAAGCTGGTTGCAGATATTGGAATAGTGGGTGCTCCAAATGCAGGGAAAAGCACACTTTTGAGTGTGATAAGTGCTGCTCAGCCAACAATAGCAAATTACCCCTTCACAACTTTACTTCCCAATCTGGGTGTGGTTTCGTTTGACTATGATTCTACAATGGTAGTAGCAGATCTGCCAGGTTTACTTGAAGGAGCACACCGCGGTTTCGGTTTGGGCCATGAGTTCCTACGGCACACTGAGAGGTGTTCCGCCCTGATACATGTCGTAGATGGCTCATCACAACAGCCAGAATTTGAGTTTGATGCAGTACGTCTGGAGTTGGAACTGTTTAGCCCTGAAATTGCTGAAAAGCCTTATGTAGTTGCGTTTAACAAAATGGACCTTCCAGATGCATATGAAAACTGGTCATCATTCAAAGAAAATTTAGAATCTCGTGGGATTCATGTTTTTTGCATGAGTGCAGTGAAAAGAGAGGGTACTCATGAAGTGAGCTGTGCTGCTTATGAGCTTCTACGAGAAAATAAAGTGGCCAAGGAGGAGTTCCCAGACCCGGTCAATTTGAATCATGTAGCTGAGATGGTGCGTAAGCAGCAAACTGCGTCTATTAATGAGTTTGAGATCACTCATGACAACAGTAGCAATACTTGGCATGTTGTGGGATCTGGGTTGCAACGCTTTGTTCAAATGACGAATTGGAGATATGTGGATTCTGGGAGAAGGTTCCAGCATGTTCTGGATGCTTGTGGCGTGAACAAATCTCTTATAAAACTTGGTGTGAAAGAAGGCGACACGGTGATTGTTGGAGATATGGAAATGGTATGGCACGATGCTGCAGATAATTCCAGTTTTTCAAGTTTTAGGAAAGGATCCACAGAAACAACCAAGTGGCCTCAATGGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003924 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009528 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009657 GO:0009658 GO:0009668 GO:0009706 GO:0009790 GO:0009791 GO:0009793 GO:0009941 GO:0009987 GO:0010027 GO:0010154 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0019866 GO:0022414 GO:0022613 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0032501 GO:0032502 GO:0034470 GO:0034641 GO:0034660 GO:0042170 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061024 GO:0061458 GO:0071704 GO:0071840 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1967 GTP1_OBG MMR_HSR1

Protein Analysis

655

Amino Acids

71.74

Weight (kDa)

5.0

Isoelectric Point (pI)

36.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP1_OBG	PF01018	203 - 356	5.4e-54	GTP1/OBG
MMR_HSR1	PF01926	360 - 479	8.3e-23	50S ribosome-binding GTPase
FeoB_N	PF02421	360 - 518	1.5e-11	Ferrous iron transport protein B
DUF1967	PF09269	559 - 628	1.8e-20	Domain of unknown function (DUF1967)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007758)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18570
fragaria_vesca	FvH4_4g17620 FvH4_4g17620 FvH4_4g17620
malus_domestica	MD16G1172900.v1.1
prunus_persica	Prupe.1G139700_v2.0.a1 Prupe.1G139700_v2.0.a1 Prupe.1G139700_v2.0.a1 Prupe.1G139700_v2.0.a1 Prupe.1G139700_v2.0.a1 Prupe.1G139700_v2.0.a1 Prupe.1G139700_v2.0.a1 Prupe.1G139700_v2.0.a1
pyrus_communis	pycom16g14500
rosa_chinensis	RchiOBHm_Chr4g0421541
rosa_laevigata	RLG00000007669
rosa_multiflora	Rmu_sc0022469.1_g000001
rosa_roxburghii	Rroxscaffold_5G00363790
rosa_rugosa	Rorug04G0172500 Rorug04G0172600
rosa_samantha	Rh4AG232900 Rh4BG235600 Rh4CG247300
rosa_wichuraiana	Rw4G020110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1826
Acc36I	ACCTGC	1 cut(s)	76
AccB1I	GGYRCC	1 cut(s)	865
AccII	CGCG	3 cut(s)	143, 612, 1263
AclI	AACGTT	1 cut(s)	117
AclWI	GGATC	3 cut(s)	1729, 1923, 1936
AcoI	YGGCCR	1 cut(s)	1590
AcsI	RAATTY	6 cut(s)	367, 377, 529, 754, 1352, 1483
AcuI	CTGAAG	2 cut(s)	146, 681
AcyI	GRCGYC	2 cut(s)	165, 866
AfaI	GTAC	3 cut(s)	783, 1371, 1540
AfiI	CCNNNNNNNGG	8 cut(s)	633, 689, 701, 889, 1044, 1103, 1187, 1612
AflIII	ACRYGT	1 cut(s)	1321
AgsI	TTSAA	6 cut(s)	511, 1250, 1477, 1624, 1745, 1914
AjnI	CCWGG	3 cut(s)	949, 1007, 1237
AjuI	GAANNNNNNNTTGG	2 cut(s)	1586, 1618
AluBI	AGCT	9 cut(s)	21, 244, 262, 524, 1054, 1066, 1554, 1570, 1634
AluI	AGCT	9 cut(s)	21, 244, 262, 524, 1054, 1066, 1554, 1570, 1634
Alw21I	GWGCWC	3 cut(s)	246, 1096, 1258
Alw26I	GTCTC	2 cut(s)	225, 828
AlwI	GGATC	3 cut(s)	1729, 1923, 1936
Ama87I	CYCGRG	3 cut(s)	46, 869, 1037
AoxI	GGCC	5 cut(s)	98, 171, 1276, 1590, 1949
ApeKI	GCWGC	6 cut(s)	315, 931, 1136, 1559, 1651, 1892
ApoI	RAATTY	6 cut(s)	367, 377, 529, 754, 1352, 1483
ArsI	GACNNNNNNTTYG	2 cut(s)	137, 169
AseI	ATTAAT	1 cut(s)	1668
Asp700I	GAANNNNTTC	1 cut(s)	976
AspA2I	CCTAGG	1 cut(s)	502
AspLEI	GCGC	3 cut(s)	143, 614, 868
AspS9I	GGNCC	6 cut(s)	52, 692, 1003, 1034, 1276, 1441
AsuC2I	CCSGG	5 cut(s)	870, 871, 927, 991, 1613
AsuHPI	GGTGA	6 cut(s)	268, 461, 506, 560, 815, 1867
AsuII	TTCGAA	1 cut(s)	772
AvaI	CYCGRG	3 cut(s)	46, 869, 1037
AvaII	GGWCC	5 cut(s)	52, 692, 1003, 1034, 1441
AvrII	CCTAGG	1 cut(s)	502
BalI	TGGCCA	1 cut(s)	1592
BamHI	GGATCC	1 cut(s)	1928
BanI	GGYRCC	1 cut(s)	865
BanII	GRGCYC	1 cut(s)	246
BauI	CACGAG	1 cut(s)	1494
Bbv12I	GWGCWC	3 cut(s)	246, 1096, 1258
BbvI	GCAGC	6 cut(s)	327, 943, 1123, 1546, 1663, 1879
BccI	CCATC	5 cut(s)	386, 440, 737, 1325, 1633
BceAI	ACGGC	4 cut(s)	475, 664, 751, 1307
BciT130I	CCWGG	3 cut(s)	951, 1009, 1239
BclI	TGATCA	1 cut(s)	805
BcnI	CCSGG	5 cut(s)	870, 871, 927, 991, 1613
BcoDI	GTCTC	2 cut(s)	225, 828
BfaI	CTAG	1 cut(s)	503
BfmI	CTRYAG	1 cut(s)	1893
BfoI	RGCGCY	1 cut(s)	869
BfuAI	ACCTGC	1 cut(s)	76
BglII	AGATCT	1 cut(s)	1231
BisI	GCNGC	9 cut(s)	279, 316, 665, 703, 932, 1137, 1560, 1652, 1893
BlnI	CCTAGG	1 cut(s)	502
BlpI	GCTNAGC	1 cut(s)	1140
BlsI	GCNGC	9 cut(s)	280, 317, 666, 704, 933, 1138, 1561, 1653, 1894
Bme1390I	CCNGG	8 cut(s)	870, 871, 927, 951, 991, 1009, 1239, 1613
Bme18I	GGWCC	5 cut(s)	52, 692, 1003, 1034, 1441
BmeT110I	CYCGRG	3 cut(s)	46, 869, 1037
BmgT120I	GGNCC	6 cut(s)	52, 692, 1003, 1034, 1276, 1441
BmiI	GGNNCC	9 cut(s)	53, 54, 693, 867, 1005, 1035, 1036, 1784, 1930
BmrFI	CCNGG	8 cut(s)	870, 871, 927, 951, 991, 1009, 1239, 1613
BmsI	GCATC	6 cut(s)	217, 931, 1354, 1441, 1789, 1879
BoxI	GACNNNNGTC	1 cut(s)	878
BplI	GAGNNNNNCTC	2 cut(s)	194, 226
BpmI	CTGGAG	3 cut(s)	932, 972, 1397
Bpu10I	CCTNAGC	1 cut(s)	837
Bpu1102I	GCTNAGC	1 cut(s)	1140
Bpu14I	TTCGAA	1 cut(s)	772
BpuMI	CCSGG	5 cut(s)	870, 871, 927, 991, 1613
BsaHI	GRCGYC	2 cut(s)	165, 866
BsaWI	WCCGGW	1 cut(s)	536
BsaXI	ACNNNNNCTCC	4 cut(s)	233, 263, 834, 864
Bsc4I	CCNNNNNNNGG	8 cut(s)	633, 689, 701, 889, 1044, 1103, 1187, 1612
Bse118I	RCCGGY	1 cut(s)	137
Bse1I	ACTGG	4 cut(s)	417, 471, 1469, 1905
Bse3DI	GCAATG	1 cut(s)	666
BseBI	CCWGG	3 cut(s)	951, 1009, 1239
BseGI	GGATG	7 cut(s)	369, 421, 442, 748, 904, 922, 1804
BseLI	CCNNNNNNNGG	8 cut(s)	633, 689, 701, 889, 1044, 1103, 1187, 1612
BseMI	GCAATG	1 cut(s)	666
BseMII	CTCAG	7 cut(s)	243, 258, 839, 1008, 1154, 1290, 1626
BseNI	ACTGG	4 cut(s)	417, 471, 1469, 1905
BseRI	GAGGAG	8 cut(s)	341, 344, 434, 437, 440, 443, 446, 1613
BseXI	GCAGC	6 cut(s)	327, 943, 1123, 1546, 1663, 1879
BsgI	GTGCAG	2 cut(s)	1047, 1542
Bsh1236I	CGCG	3 cut(s)	143, 612, 1263
Bsh1285I	CGRYCG	1 cut(s)	610
BshFI	GGCC	5 cut(s)	100, 173, 1278, 1592, 1951
BshNI	GGYRCC	1 cut(s)	865
BsiEI	CGRYCG	1 cut(s)	610
BsiHKAI	GWGCWC	3 cut(s)	246, 1096, 1258
BsiHKCI	CYCGRG	3 cut(s)	46, 869, 1037
BsiSI	CCGG	6 cut(s)	138, 537, 870, 926, 990, 1613
BslFI	GGGAC	7 cut(s)	38, 650, 705, 886, 989, 1006, 1020
BslI	CCNNNNNNNGG	8 cut(s)	633, 689, 701, 889, 1044, 1103, 1187, 1612
BsmAI	GTCTC	2 cut(s)	225, 828
BsmBI	CGTCTC	1 cut(s)	225
BsmFI	GGGAC	7 cut(s)	38, 650, 705, 886, 989, 1006, 1020
BsnI	GGCC	5 cut(s)	100, 173, 1278, 1592, 1951
BsoBI	CYCGRG	3 cut(s)	46, 869, 1037
Bsp119I	TTCGAA	1 cut(s)	772
Bsp1286I	GDGCHC	3 cut(s)	246, 1096, 1258
Bsp143I	GATC	5 cut(s)	805, 1231, 1680, 1721, 1928
Bsp1720I	GCTNAGC	1 cut(s)	1140
Bsp19I	CCATGG	1 cut(s)	36
BspANI	GGCC	5 cut(s)	100, 173, 1278, 1592, 1951
BspCNI	CTCAG	7 cut(s)	244, 259, 838, 1009, 1153, 1291, 1627
BspFNI	CGCG	3 cut(s)	143, 612, 1263
BspHI	TCATGA	2 cut(s)	1543, 1687
BspLI	GGNNCC	9 cut(s)	53, 54, 693, 867, 1005, 1035, 1036, 1784, 1930
BspMAI	CTGCAG	1 cut(s)	1897
BspMI	ACCTGC	1 cut(s)	76
BspPI	GGATC	3 cut(s)	1729, 1923, 1936
BspT104I	TTCGAA	1 cut(s)	772
BspT107I	GGYRCC	1 cut(s)	865
BsrDI	GCAATG	1 cut(s)	666
BsrFI	RCCGGY	1 cut(s)	137
BsrI	ACTGG	4 cut(s)	417, 471, 1469, 1905
BssAI	RCCGGY	1 cut(s)	137
BssMI	GATC	5 cut(s)	805, 1231, 1680, 1721, 1928
BssNI	GRCGYC	2 cut(s)	165, 866
BssSI	CACGAG	1 cut(s)	1494
BssT1I	CCWWGG	4 cut(s)	36, 502, 627, 1593
Bst2BI	CACGAG	1 cut(s)	1494
Bst2UI	CCWGG	3 cut(s)	951, 1009, 1239
Bst4CI	ACNGT	6 cut(s)	443, 494, 877, 1389, 1697, 1855
Bst6I	CTCTTC	2 cut(s)	318, 808
BstACI	GRCGYC	2 cut(s)	165, 866
BstBI	TTCGAA	1 cut(s)	772
BstC8I	GCNNGC	5 cut(s)	43, 68, 191, 588, 936
BstDEI	CTNAG	8 cut(s)	252, 267, 825, 837, 1017, 1140, 1299, 1635
BstDSI	CCRYGG	2 cut(s)	36, 1261
BstF5I	GGATG	7 cut(s)	369, 421, 442, 748, 904, 922, 1804
BstFNI	CGCG	3 cut(s)	143, 612, 1263
BstH2I	RGCGCY	1 cut(s)	869
BstHHI	GCGC	3 cut(s)	143, 614, 868
BstKTI	GATC	5 cut(s)	808, 1234, 1683, 1724, 1931
BstMAI	GTCTC	2 cut(s)	225, 828
BstMBI	GATC	5 cut(s)	805, 1231, 1680, 1721, 1928
BstMCI	CGRYCG	1 cut(s)	610
BstMWI	GCNNNNNNNGC	3 cut(s)	928, 1100, 1651
BstNI	CCWGG	3 cut(s)	951, 1009, 1239
BstNSI	RCATGY	3 cut(s)	1325, 1715, 1793
BstPAI	GACNNNNGTC	1 cut(s)	878
BstSCI	CCNGG	8 cut(s)	868, 869, 925, 949, 989, 1007, 1237, 1611
BstSFI	CTRYAG	1 cut(s)	1893
BstUI	CGCG	3 cut(s)	143, 612, 1263
BstV1I	GCAGC	6 cut(s)	327, 943, 1123, 1546, 1663, 1879
BstX2I	RGATCY	3 cut(s)	1231, 1721, 1928
BstYI	RGATCY	3 cut(s)	1231, 1721, 1928
BsuRI	GGCC	5 cut(s)	100, 173, 1278, 1592, 1951
BtgI	CCRYGG	2 cut(s)	36, 1261
BtsCI	GGATG	7 cut(s)	369, 421, 442, 748, 904, 922, 1804
BtsI	GCAGTG	1 cut(s)	1530
BtsIMutI	CAGTG	3 cut(s)	448, 1296, 1530
BveI	ACCTGC	1 cut(s)	76
Cac8I	GCNNGC	5 cut(s)	43, 68, 191, 588, 936
CciI	TCATGA	2 cut(s)	1543, 1687
CfoI	GCGC	3 cut(s)	143, 614, 868
Cfr10I	RCCGGY	1 cut(s)	137
Cfr13I	GGNCC	6 cut(s)	52, 692, 1003, 1034, 1276, 1441
Cfr42I	CCGCGG	1 cut(s)	1264
Cfr9I	CCCGGG	1 cut(s)	869
CseI	GACGC	3 cut(s)	154, 219, 1650
Csp6I	GTAC	3 cut(s)	782, 1370, 1539
CviQI	GTAC	3 cut(s)	782, 1370, 1539
DdeI	CTNAG	8 cut(s)	252, 267, 825, 837, 1017, 1140, 1299, 1635
DinI	GGCGCC	1 cut(s)	867
DpnI	GATC	5 cut(s)	807, 1233, 1682, 1723, 1930
DpnII	GATC	5 cut(s)	805, 1231, 1680, 1721, 1928
DraI	TTTAAA	1 cut(s)	985
EaeI	YGGCCR	1 cut(s)	1590
Eam1104I	CTCTTC	2 cut(s)	318, 808
EarI	CTCTTC	2 cut(s)	318, 808
EciI	GGCGGA	1 cut(s)	1300
Ecl136II	GAGCTC	1 cut(s)	244
Eco130I	CCWWGG	4 cut(s)	36, 502, 627, 1593
Eco147I	AGGCCT	1 cut(s)	100
Eco24I	GRGCYC	1 cut(s)	246
Eco47I	GGWCC	5 cut(s)	52, 692, 1003, 1034, 1441
Eco53kI	GAGCTC	1 cut(s)	244
Eco57I	CTGAAG	2 cut(s)	146, 681
Eco88I	CYCGRG	3 cut(s)	46, 869, 1037
EcoICRI	GAGCTC	1 cut(s)	244
EcoO109I	RGGNCCY	4 cut(s)	52, 692, 1003, 1034
EcoRI	GAATTC	2 cut(s)	367, 754
EcoRII	CCWGG	3 cut(s)	949, 1007, 1237
EcoT14I	CCWWGG	4 cut(s)	36, 502, 627, 1593
EcoT22I	ATGCAT	1 cut(s)	1456
EcoT38I	GRGCYC	1 cut(s)	246
EgeI	GGCGCC	1 cut(s)	867
EheI	GGCGCC	1 cut(s)	867
ErhI	CCWWGG	4 cut(s)	36, 502, 627, 1593
Esp3I	CGTCTC	1 cut(s)	225
FaqI	GGGAC	7 cut(s)	38, 650, 705, 886, 989, 1006, 1020
FauI	CCCGC	3 cut(s)	660, 883, 956
FauNDI	CATATG	1 cut(s)	1456
FbaI	TGATCA	1 cut(s)	805
Fnu4HI	GCNGC	9 cut(s)	279, 316, 665, 703, 932, 1137, 1560, 1652, 1893
FokI	GGATG	7 cut(s)	376, 428, 449, 755, 909, 911, 1811
FriOI	GRGCYC	1 cut(s)	246
Fsp4HI	GCNGC	9 cut(s)	279, 316, 665, 703, 932, 1137, 1560, 1652, 1893
FspBI	CTAG	1 cut(s)	503
GlaI	GCGC	3 cut(s)	142, 613, 867
GluI	GCNGC	9 cut(s)	279, 316, 665, 703, 932, 1137, 1560, 1652, 1893
GsuI	CTGGAG	3 cut(s)	932, 972, 1397
HaeII	RGCGCY	1 cut(s)	869
HaeIII	GGCC	5 cut(s)	100, 173, 1278, 1592, 1951
HapII	CCGG	6 cut(s)	138, 537, 870, 926, 990, 1613
HgaI	GACGC	3 cut(s)	154, 219, 1650
HhaI	GCGC	3 cut(s)	143, 614, 868
Hin1I	GRCGYC	2 cut(s)	165, 866
Hin6I	GCGC	3 cut(s)	141, 612, 866
HinP1I	GCGC	3 cut(s)	141, 612, 866
HindIII	AAGCTT	1 cut(s)	19
HinfI	GANTC	8 cut(s)	61, 269, 404, 1211, 1490, 1501, 1624, 1769
HpaII	CCGG	6 cut(s)	138, 537, 870, 926, 990, 1613
HphI	GGTGA	6 cut(s)	268, 461, 506, 560, 815, 1867
Hpy166II	GTNNAC	2 cut(s)	1244, 1813
Hpy188I	TCNGA	7 cut(s)	75, 96, 253, 268, 409, 828, 884
Hpy188III	TCNNGA	8 cut(s)	224, 911, 1037, 1376, 1448, 1544, 1688, 1796
Hpy8I	GTNNAC	2 cut(s)	1244, 1813
Hpy99I	CGWCG	6 cut(s)	170, 235, 323, 491, 494, 710
HpyCH4III	ACNGT	6 cut(s)	443, 494, 877, 1389, 1697, 1855
HpyCH4IV	ACGT	3 cut(s)	117, 849, 1372
HpyF10VI	GCNNNNNNNGC	3 cut(s)	928, 1100, 1651
HpyF3I	CTNAG	8 cut(s)	252, 267, 825, 837, 1017, 1140, 1299, 1635
HpySE526I	ACGT	3 cut(s)	117, 849, 1372
Hsp92I	GRCGYC	2 cut(s)	165, 866
HspAI	GCGC	3 cut(s)	141, 612, 866
KasI	GGCGCC	1 cut(s)	865
KflI	GGGWCCC	2 cut(s)	52, 1034
Ksp22I	TGATCA	1 cut(s)	805
KspI	CCGCGG	1 cut(s)	1264
Kzo9I	GATC	5 cut(s)	805, 1231, 1680, 1721, 1928
LmnI	GCTCC	4 cut(s)	241, 1051, 1099, 1253
Lsp1109I	GCAGC	6 cut(s)	327, 943, 1123, 1546, 1663, 1879
LweI	GCATC	6 cut(s)	217, 931, 1354, 1441, 1789, 1879
MaeI	CTAG	1 cut(s)	503
MaeII	ACGT	3 cut(s)	117, 849, 1372
MaeIII	GTNAC	1 cut(s)	88
MalI	GATC	5 cut(s)	807, 1233, 1682, 1723, 1930
MboI	GATC	5 cut(s)	805, 1231, 1680, 1721, 1928
MboII	GAAGA	3 cut(s)	335, 347, 825
MflI	RGATCY	3 cut(s)	1231, 1721, 1928
MhlI	GDGCHC	3 cut(s)	246, 1096, 1258
MlsI	TGGCCA	1 cut(s)	1592
MluNI	TGGCCA	1 cut(s)	1592
Mly113I	GGCGCC	1 cut(s)	866
MlyI	GAGTC	2 cut(s)	55, 278
MmeI	TCCRAC	4 cut(s)	219, 1029, 1362, 1843
Mox20I	TGGCCA	1 cut(s)	1592
Mph1103I	ATGCAT	1 cut(s)	1456
MroXI	GAANNNNTTC	1 cut(s)	976
MscI	TGGCCA	1 cut(s)	1592
MseI	TTAA	5 cut(s)	311, 858, 984, 1431, 1668
Msp20I	TGGCCA	1 cut(s)	1592
MspA1I	CMGCKG	2 cut(s)	590, 1263
MspI	CCGG	6 cut(s)	138, 537, 870, 926, 990, 1613
MspR9I	CCNGG	8 cut(s)	870, 871, 927, 951, 991, 1009, 1239, 1613
MvaI	CCWGG	3 cut(s)	951, 1009, 1239
MvnI	CGCG	3 cut(s)	143, 612, 1263
MwoI	GCNNNNNNNGC	3 cut(s)	928, 1100, 1651
NarI	GGCGCC	1 cut(s)	866
NciI	CCSGG	5 cut(s)	870, 871, 927, 991, 1613
NcoI	CCATGG	1 cut(s)	36
NdeI	CATATG	1 cut(s)	1456
NdeII	GATC	5 cut(s)	805, 1231, 1680, 1721, 1928
NlaIV	GGNNCC	9 cut(s)	53, 54, 693, 867, 1005, 1035, 1036, 1784, 1930
NsiI	ATGCAT	1 cut(s)	1456
NspI	RCATGY	3 cut(s)	1325, 1715, 1793
NspV	TTCGAA	1 cut(s)	772
PagI	TCATGA	2 cut(s)	1543, 1687
PceI	AGGCCT	1 cut(s)	100
PciI	ACATGT	1 cut(s)	1321
PdmI	GAANNNNTTC	1 cut(s)	976
PfeI	GAWTC	6 cut(s)	404, 1211, 1490, 1501, 1624, 1769
PflFI	GACNNNGTC	1 cut(s)	1613
PfoI	TCCNGGA	1 cut(s)	989
PkrI	GCNGC	9 cut(s)	280, 317, 666, 704, 933, 1138, 1561, 1653, 1894
PleI	GAGTC	2 cut(s)	55, 277
PluTI	GGCGCC	1 cut(s)	869
PpsI	GAGTC	2 cut(s)	55, 277
PpuMI	RGGWCCY	4 cut(s)	52, 692, 1003, 1034
PscI	ACATGT	1 cut(s)	1321
PshAI	GACNNNNGTC	1 cut(s)	878
PshBI	ATTAAT	1 cut(s)	1668
PsiI	TTATAA	1 cut(s)	1826
Psp124BI	GAGCTC	1 cut(s)	246
Psp1406I	AACGTT	1 cut(s)	117
Psp5II	RGGWCCY	4 cut(s)	52, 692, 1003, 1034
Psp6I	CCWGG	3 cut(s)	949, 1007, 1237
PspGI	CCWGG	3 cut(s)	949, 1007, 1237
PspN4I	GGNNCC	9 cut(s)	53, 54, 693, 867, 1005, 1035, 1036, 1784, 1930
PspPI	GGNCC	6 cut(s)	52, 692, 1003, 1034, 1276, 1441
PspPPI	RGGWCCY	4 cut(s)	52, 692, 1003, 1034
PstI	CTGCAG	1 cut(s)	1897
PsuI	RGATCY	3 cut(s)	1231, 1721, 1928
PsyI	GACNNNGTC	1 cut(s)	1613
RsaI	GTAC	3 cut(s)	783, 1371, 1540
RsaNI	GTAC	3 cut(s)	782, 1370, 1539
SacI	GAGCTC	1 cut(s)	246
SacII	CCGCGG	1 cut(s)	1264
SaqAI	TTAA	5 cut(s)	311, 858, 984, 1431, 1668
SatI	GCNGC	9 cut(s)	279, 316, 665, 703, 932, 1137, 1560, 1652, 1893
Sau3AI	GATC	5 cut(s)	805, 1231, 1680, 1721, 1928
Sau96I	GGNCC	6 cut(s)	52, 692, 1003, 1034, 1276, 1441
SchI	GAGTC	2 cut(s)	55, 278
ScrFI	CCNGG	8 cut(s)	870, 871, 927, 951, 991, 1009, 1239, 1613
SduI	GDGCHC	3 cut(s)	246, 1096, 1258
SfaNI	GCATC	6 cut(s)	217, 931, 1354, 1441, 1789, 1879
SfcI	CTRYAG	1 cut(s)	1893
SfoI	GGCGCC	1 cut(s)	867
Sfr303I	CCGCGG	1 cut(s)	1264
SfuI	TTCGAA	1 cut(s)	772
SgrBI	CCGCGG	1 cut(s)	1264
SinI	GGWCC	5 cut(s)	52, 692, 1003, 1034, 1441
SmaI	CCCGGG	1 cut(s)	871
SseBI	AGGCCT	1 cut(s)	100
SspDI	GGCGCC	1 cut(s)	865
SspMI	CTAG	1 cut(s)	503
SstI	GAGCTC	1 cut(s)	246
StuI	AGGCCT	1 cut(s)	100
StyD4I	CCNGG	8 cut(s)	868, 869, 925, 949, 989, 1007, 1237, 1611
StyI	CCWWGG	4 cut(s)	36, 502, 627, 1593
TaaI	ACNGT	6 cut(s)	443, 494, 877, 1389, 1697, 1855
TaiI	ACGT	3 cut(s)	120, 852, 1375
TaqI	TCGA	6 cut(s)	133, 168, 225, 606, 749, 772
TauI	GCSGC	3 cut(s)	281, 667, 705
TfiI	GAWTC	6 cut(s)	404, 1211, 1490, 1501, 1624, 1769
Tru1I	TTAA	5 cut(s)	311, 858, 984, 1431, 1668
Tru9I	TTAA	5 cut(s)	311, 858, 984, 1431, 1668
TscAI	CASTG	3 cut(s)	448, 1303, 1530
TseI	GCWGC	6 cut(s)	315, 931, 1136, 1559, 1651, 1892
TspDTI	ATGAA	5 cut(s)	380, 767, 1473, 1493, 1560
TspGWI	ACGGA	3 cut(s)	148, 654, 723
TspMI	CCCGGG	1 cut(s)	869
TspRI	CASTG	3 cut(s)	448, 1303, 1530
Tth111I	GACNNNGTC	1 cut(s)	1613
VpaK11BI	GGWCC	5 cut(s)	52, 692, 1003, 1034, 1441
VspI	ATTAAT	1 cut(s)	1668
XapI	RAATTY	6 cut(s)	367, 377, 529, 754, 1352, 1483
XceI	RCATGY	3 cut(s)	1325, 1715, 1793
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1189
XmaI	CCCGGG	1 cut(s)	869
XmaJI	CCTAGG	1 cut(s)	502
XmnI	GAANNNNTTC	1 cut(s)	976
XspI	CTAG	1 cut(s)	503
Zsp2I	ATGCAT	1 cut(s)	1456

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.