pycom16g16800

Catalyzes the formation of formate and 2-keto-4- methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5- methylthiopentene (DHK-MTPene)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014076
Physical Location & Seq
Forward (+)
12819260 .. 12820786
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 558 bp
ATGGAGGCATGGTTCATGGACGACAGCGATTCAGACCCAAGGCTTCCTCACCATCGCAACCCCATAGAGTTTATTCCTTTGGACCATTTGGCAGAGCTGGGTGTGCTCCACTGGCGCTTGAACCCAACGGATTATGAAAACGATCAAGAACTGCGCAAAATTAGGGAAACCAGGGGATATAATTACATGGATTTGCTTGACATATGCCCAGAGAAATTGAACAATTATGAGGAGAAGTTGAAGAACTTCTACACAGAGCACATACATGCTGATGAGGAGATTCGCTACTGTTTAGAGGGAAGCGGGTACTTTGATGTCCGAGGGAAGGATGACCGTTGGATTCGAATTTGGATTAAGGCCGGTGATCTTATCATTTTGCCCGCTGGGATTTACCACAGGTTCACCCTTGATACCACCAATTATGTTAAGTTGATGAGGCTGTTTATGGGAGAGCCTGTGTGGACTGCGTATAATCGGCCACAGGAAGAACATCCGGCCAGGAAGGAGTATGTAGAGAGCTTCACTAAGAAAGTAGGAGTGGCATTAAAAGCTCATTAA

Protein Analysis

186

Amino Acids

22.17

Weight (kDa)

5.59

Isoelectric Point (pI)

34.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARD PF03079 3 - 156 1e-59 ARD/ARD' family
AraC_binding PF02311 83 - 161 2.4e-07 AraC-like ligand binding domain
Cupin_2 PF07883 86 - 144 1.2e-09 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014076)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43850
fragaria_vesca FvH4_4g01130
malus_domestica MD16G1199500.v1.1
prunus_persica Prupe.1G021000_v2.0.a1 Prupe.1G021000_v2.0.a1
pyrus_communis pycom16g16800
rosa_chinensis RchiOBHm_Chr4g0387701
rosa_laevigata RLG00000010100
rosa_multiflora Rmu_sc0003953.1_g000019 Rmu_sc0006621.1_g000002
rosa_roxburghii Rroxscaffold_5G00334350
rosa_rugosa Rorug03G0312800
rosa_samantha Rh4AG017800 Rh4BG012700 Rh4CG019000 Rh4DG013600
rosa_wichuraiana Rw4G001160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 155
AciI CCGC 2 cut(s) 303, 381
AcoI YGGCCR 2 cut(s) 476, 495
AcsI RAATTY 1 cut(s) 345
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 3 cut(s) 121, 220, 241
AjnI CCWGG 2 cut(s) 170, 497
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 3 cut(s) 97, 519, 551
AluI AGCT 3 cut(s) 97, 519, 551
Alw21I GWGCWC 2 cut(s) 108, 261
AoxI GGCC 3 cut(s) 357, 476, 495
ApoI RAATTY 1 cut(s) 345
Asp700I GAANNNNTTC 1 cut(s) 245
AspLEI GCGC 2 cut(s) 117, 156
AspS9I GGNCC 1 cut(s) 82
AsuHPI GGTGA 3 cut(s) 41, 374, 394
AsuII TTCGAA 1 cut(s) 343
AvaII GGWCC 1 cut(s) 82
BarI GAAGNNNNNNTAC 2 cut(s) 233, 265
Bbv12I GWGCWC 2 cut(s) 108, 261
BccI CCATC 1 cut(s) 60
BciT130I CCWGG 2 cut(s) 172, 499
BfoI RGCGCY 1 cut(s) 118
Bme1390I CCNGG 2 cut(s) 172, 499
Bme18I GGWCC 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 82
BmrFI CCNGG 2 cut(s) 172, 499
Bpu14I TTCGAA 1 cut(s) 343
BsaJI CCNNGG 3 cut(s) 38, 171, 319
BsaXI ACNNNNNCTCC 3 cut(s) 26, 441, 471
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 1 cut(s) 116
BseBI CCWGG 2 cut(s) 172, 499
BseDI CCNNGG 3 cut(s) 38, 171, 319
BseGI GGATG 2 cut(s) 334, 490
BseLI CCNNNNNNNGG 1 cut(s) 325
BseNI ACTGG 1 cut(s) 116
BseRI GAGGAG 2 cut(s) 245, 290
BseYI CCCAGC 2 cut(s) 97, 383
BshFI GGCC 3 cut(s) 359, 478, 497
BsiHKAI GWGCWC 2 cut(s) 108, 261
BsiSI CCGG 2 cut(s) 360, 494
BslI CCNNNNNNNGG 1 cut(s) 325
BsnI GGCC 3 cut(s) 359, 478, 497
Bsp119I TTCGAA 1 cut(s) 343
Bsp1286I GDGCHC 2 cut(s) 108, 261
Bsp143I GATC 2 cut(s) 142, 364
BspACI CCGC 2 cut(s) 303, 381
BspANI GGCC 3 cut(s) 359, 478, 497
BspT104I TTCGAA 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 1 cut(s) 116
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 3 cut(s) 38, 171, 319
BssMI GATC 2 cut(s) 142, 364
BssT1I CCWWGG 1 cut(s) 38
Bst2UI CCWGG 2 cut(s) 172, 499
Bst4CI ACNGT 2 cut(s) 290, 335
BstBI TTCGAA 1 cut(s) 343
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 1 cut(s) 525
BstF5I GGATG 2 cut(s) 334, 490
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 2 cut(s) 117, 156
BstKTI GATC 2 cut(s) 145, 367
BstMBI GATC 2 cut(s) 142, 364
BstMWI GCNNNNNNNGC 3 cut(s) 103, 112, 548
BstNI CCWGG 2 cut(s) 172, 499
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 2 cut(s) 170, 497
BsuRI GGCC 3 cut(s) 359, 478, 497
BtgZI GCGATG 1 cut(s) 38
BtsCI GGATG 2 cut(s) 334, 490
BtsIMutI CAGTG 1 cut(s) 109
Cac8I GCNNGC 1 cut(s) 381
CfoI GCGC 2 cut(s) 117, 156
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 82
Csp6I GTAC 1 cut(s) 307
CviAII CATG 4 cut(s) 9, 16, 187, 266
CviJI RGCY 9 cut(s) 43, 97, 359, 439, 454, 478, 497, 519, 551
CviKI_1 RGCY 9 cut(s) 43, 97, 359, 439, 454, 478, 497, 519, 551
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 1 cut(s) 525
DpnI GATC 2 cut(s) 144, 366
DpnII GATC 2 cut(s) 142, 364
EaeI YGGCCR 2 cut(s) 476, 495
Eco130I CCWWGG 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 170, 497
EcoT14I CCWWGG 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 38
FaeI CATG 4 cut(s) 12, 19, 190, 269
FatI CATG 4 cut(s) 8, 15, 186, 265
FauI CCCGC 2 cut(s) 296, 388
FauNDI CATATG 1 cut(s) 203
FokI GGATG 2 cut(s) 341, 477
FspI TGCGCA 1 cut(s) 155
GlaI GCGC 2 cut(s) 116, 155
GsaI CCCAGC 2 cut(s) 101, 387
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 3 cut(s) 359, 478, 497
HapII CCGG 2 cut(s) 360, 494
HhaI GCGC 2 cut(s) 117, 156
Hin1II CATG 4 cut(s) 12, 19, 190, 269
Hin6I GCGC 2 cut(s) 115, 154
HinP1I GCGC 2 cut(s) 115, 154
HinfI GANTC 3 cut(s) 29, 280, 340
HpaII CCGG 2 cut(s) 360, 494
HphI GGTGA 3 cut(s) 41, 374, 394
Hpy166II GTNNAC 2 cut(s) 402, 462
Hpy188I TCNGA 2 cut(s) 34, 320
Hpy188III TCNNGA 1 cut(s) 146
Hpy8I GTNNAC 2 cut(s) 402, 462
HpyAV CCTTC 2 cut(s) 319, 496
HpyCH4III ACNGT 2 cut(s) 290, 335
HpyF10VI GCNNNNNNNGC 3 cut(s) 103, 112, 548
HpyF3I CTNAG 1 cut(s) 525
Hsp92II CATG 4 cut(s) 12, 19, 190, 269
HspAI GCGC 2 cut(s) 115, 154
Kzo9I GATC 2 cut(s) 142, 364
LmnI GCTCC 1 cut(s) 111
MalI GATC 2 cut(s) 144, 366
MboI GATC 2 cut(s) 142, 364
MboII GAAGA 2 cut(s) 253, 497
MhlI GDGCHC 2 cut(s) 108, 261
MluCI AATT 6 cut(s) 159, 181, 215, 223, 345, 418
MmeI TCCRAC 1 cut(s) 317
MnlI CCTC 6 cut(s) 57, 223, 268, 289, 314, 429
MroXI GAANNNNTTC 1 cut(s) 245
MseI TTAA 4 cut(s) 354, 426, 545, 556
MslI CAYNNNNRTG 2 cut(s) 264, 270
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 2 cut(s) 360, 494
MspR9I CCNGG 2 cut(s) 172, 499
MvaI CCWGG 2 cut(s) 172, 499
MwoI GCNNNNNNNGC 3 cut(s) 103, 112, 548
NdeI CATATG 1 cut(s) 203
NdeII GATC 2 cut(s) 142, 364
NlaIII CATG 4 cut(s) 12, 19, 190, 269
NsbI TGCGCA 1 cut(s) 155
NspI RCATGY 1 cut(s) 269
NspV TTCGAA 1 cut(s) 343
PdmI GAANNNNTTC 1 cut(s) 245
PfeI GAWTC 3 cut(s) 29, 280, 340
Psp6I CCWGG 2 cut(s) 170, 497
PspFI CCCAGC 2 cut(s) 97, 383
PspGI CCWGG 2 cut(s) 170, 497
PspPI GGNCC 1 cut(s) 82
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
RseI CAYNNNNRTG 2 cut(s) 264, 270
SaqAI TTAA 4 cut(s) 354, 426, 545, 556
Sau3AI GATC 2 cut(s) 142, 364
Sau96I GGNCC 1 cut(s) 82
ScrFI CCNGG 2 cut(s) 172, 499
SduI GDGCHC 2 cut(s) 108, 261
SetI ASST 4 cut(s) 99, 401, 521, 553
SfuI TTCGAA 1 cut(s) 343
SinI GGWCC 1 cut(s) 82
SmiMI CAYNNNNRTG 2 cut(s) 264, 270
Sse9I AATT 6 cut(s) 159, 181, 215, 223, 345, 418
SsiI CCGC 2 cut(s) 303, 381
StyD4I CCNGG 2 cut(s) 170, 497
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 2 cut(s) 290, 335
TaqI TCGA 1 cut(s) 343
TasI AATT 6 cut(s) 159, 181, 215, 223, 345, 418
TfiI GAWTC 3 cut(s) 29, 280, 340
Tru1I TTAA 4 cut(s) 354, 426, 545, 556
Tru9I TTAA 4 cut(s) 354, 426, 545, 556
TscAI CASTG 1 cut(s) 116
TspDTI ATGAA 2 cut(s) 4, 150
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 82
XapI RAATTY 1 cut(s) 345
XceI RCATGY 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.