Rw4G001160

Catalyzes the formation of formate and 2-keto-4- methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5- methylthiopentene (DHK-MTPene)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014076
Physical Location & Seq
Forward (+)
2188618 .. 2191789
3172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G001160.1

Sequence Viewer

Length: 564 bp
ATGGCGATGGAGGCATGGTTCATGGATGAGAGCGATGCAGACCCGAGGCTTCCTCACCATCTGAACCCCAAAGAGTTTGTTCCTTTGGACTATTTGGCAGAGCTGGGTGTGCTCTACTGGCACTTGAACCCCAAGGACTATGAGAACGATGAACAACTGCGCCAGATAAGAGAAACCAGGGGATATAATTACATGGATTTGCTTGATATATGCCCGGAGAAATTGGAGAACTATGAGGACAAGCTGAAGAACTTCTACACTGAGCACATTCATGGTGATGAAGAGATACGTTACTGTTTAGAAGGATGCGGGTACTTTGATGTCCGAGACAAGGATGACCGTTGGATTAGAATCTGGATTAAGGCCGGTGATCTTATCATTTTACCTGCCGGAATTTACCACAGGTTCACCCTAGATACCAGCAACTATATCAAGTTGATGAGGTTGTTTGTGGGAGAGCCTGTGTGGACTGCTTACAATCGACCGCAGGAAGAACATCCGGCTAGGAAGGAGTACATTGAGAGCTTCACTAAGAAAGTTGGAGTGGCATTGGAAGCTCATTAA

Protein Analysis

187

Amino Acids

22.43

Weight (kDa)

5.02

Isoelectric Point (pI)

35.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARD PF03079 5 - 158 8.4e-61 ARD/ARD' family
AraC_binding PF02311 85 - 146 9.4e-06 AraC-like ligand binding domain
Cupin_2 PF07883 88 - 142 8.6e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014076)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43850
fragaria_vesca FvH4_4g01130
malus_domestica MD16G1199500.v1.1
prunus_persica Prupe.1G021000_v2.0.a1 Prupe.1G021000_v2.0.a1
pyrus_communis pycom16g16800
rosa_chinensis RchiOBHm_Chr4g0387701
rosa_laevigata RLG00000010100
rosa_multiflora Rmu_sc0003953.1_g000019 Rmu_sc0006621.1_g000002
rosa_roxburghii Rroxscaffold_5G00334350
rosa_rugosa Rorug03G0312800
rosa_samantha Rh4AG017800 Rh4BG012700 Rh4CG019000 Rh4DG013600
rosa_wichuraiana Rw4G001160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 394
AciI CCGC 2 cut(s) 309, 485
AcsI RAATTY 1 cut(s) 393
AcuI CTGAAG 1 cut(s) 266
AfaI GTAC 2 cut(s) 314, 515
AfiI CCNNNNNNNGG 1 cut(s) 331
AgsI TTSAA 1 cut(s) 127
AjnI CCWGG 1 cut(s) 176
AloI GAACNNNNNNTCC 1 cut(s) 34
AluBI AGCT 4 cut(s) 103, 244, 525, 557
AluI AGCT 4 cut(s) 103, 244, 525, 557
Alw21I GWGCWC 2 cut(s) 114, 267
Alw26I GTCTC 1 cut(s) 321
Ama87I CYCGRG 1 cut(s) 43
AoxI GGCC 1 cut(s) 363
ApoI RAATTY 1 cut(s) 393
Asp700I GAANNNNTTC 1 cut(s) 251
AspLEI GCGC 1 cut(s) 162
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 4 cut(s) 47, 287, 380, 400
AvaI CYCGRG 1 cut(s) 43
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
Bbv12I GWGCWC 2 cut(s) 114, 267
BccI CCATC 1 cut(s) 66
BciT130I CCWGG 1 cut(s) 178
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 1 cut(s) 321
BfaI CTAG 2 cut(s) 413, 504
BfuAI ACCTGC 1 cut(s) 394
Bme1390I CCNGG 2 cut(s) 178, 215
BmeT110I CYCGRG 1 cut(s) 43
BmrFI CCNGG 2 cut(s) 178, 215
BmsI GCATC 2 cut(s) 25, 296
BplI GAGNNNNNCTC 2 cut(s) 37, 69
BpuMI CCSGG 1 cut(s) 215
BsaBI GATNNNNATC 1 cut(s) 350
BsaJI CCNNGG 3 cut(s) 44, 132, 177
BsaXI ACNNNNNCTCC 3 cut(s) 32, 447, 477
Bsc4I CCNNNNNNNGG 1 cut(s) 331
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 1 cut(s) 122
Bse8I GATNNNNATC 1 cut(s) 350
BseBI CCWGG 1 cut(s) 178
BseDI CCNNGG 3 cut(s) 44, 132, 177
BseGI GGATG 4 cut(s) 31, 311, 340, 496
BseJI GATNNNNATC 1 cut(s) 350
BseLI CCNNNNNNNGG 1 cut(s) 331
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 122
BseYI CCCAGC 1 cut(s) 103
Bsh1285I CGRYCG 1 cut(s) 485
BshFI GGCC 1 cut(s) 365
BsiEI CGRYCG 1 cut(s) 485
BsiHKAI GWGCWC 2 cut(s) 114, 267
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 4 cut(s) 215, 366, 390, 500
BslI CCNNNNNNNGG 1 cut(s) 331
BsmAI GTCTC 1 cut(s) 321
BsnI GGCC 1 cut(s) 365
BsoBI CYCGRG 1 cut(s) 43
Bsp1286I GDGCHC 2 cut(s) 114, 267
Bsp143I GATC 1 cut(s) 370
BspACI CCGC 2 cut(s) 309, 485
BspANI GGCC 1 cut(s) 365
BspCNI CTCAG 1 cut(s) 253
BspMI ACCTGC 1 cut(s) 394
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 3 cut(s) 44, 132, 177
BssMI GATC 1 cut(s) 370
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 178
Bst4CI ACNGT 2 cut(s) 296, 341
Bst6I CTCTTC 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 261, 531
BstF5I GGATG 4 cut(s) 31, 311, 340, 496
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 1 cut(s) 373
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 1 cut(s) 370
BstMCI CGRYCG 1 cut(s) 485
BstMWI GCNNNNNNNGC 4 cut(s) 11, 109, 118, 554
BstNI CCWGG 1 cut(s) 178
BstSCI CCNGG 2 cut(s) 176, 213
BsuRI GGCC 1 cut(s) 365
BtgZI GCGATG 2 cut(s) 20, 48
BtsCI GGATG 4 cut(s) 31, 311, 340, 496
BtsIMutI CAGTG 1 cut(s) 258
BveI ACCTGC 1 cut(s) 394
CfoI GCGC 1 cut(s) 162
Cfr10I RCCGGY 1 cut(s) 365
Csp6I GTAC 2 cut(s) 313, 514
CviAII CATG 4 cut(s) 15, 22, 193, 272
CviJI RGCY 8 cut(s) 49, 103, 244, 365, 460, 503, 525, 557
CviKI_1 RGCY 8 cut(s) 49, 103, 244, 365, 460, 503, 525, 557
CviQI GTAC 2 cut(s) 313, 514
DdeI CTNAG 2 cut(s) 261, 531
DpnI GATC 1 cut(s) 372
DpnII GATC 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco130I CCWWGG 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 266
Eco88I CYCGRG 1 cut(s) 43
EcoRII CCWGG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 4 cut(s) 18, 25, 196, 275
FatI CATG 4 cut(s) 14, 21, 192, 271
FauI CCCGC 1 cut(s) 302
FokI GGATG 4 cut(s) 38, 318, 347, 483
FspBI CTAG 2 cut(s) 413, 504
GlaI GCGC 1 cut(s) 161
GsaI CCCAGC 1 cut(s) 107
HaeIII GGCC 1 cut(s) 365
HapII CCGG 4 cut(s) 215, 366, 390, 500
HhaI GCGC 1 cut(s) 162
Hin1II CATG 4 cut(s) 18, 25, 196, 275
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HinfI GANTC 1 cut(s) 351
HpaII CCGG 4 cut(s) 215, 366, 390, 500
HphI GGTGA 4 cut(s) 47, 287, 380, 400
Hpy166II GTNNAC 2 cut(s) 408, 468
Hpy188I TCNGA 2 cut(s) 63, 326
Hpy188III TCNNGA 1 cut(s) 355
Hpy8I GTNNAC 2 cut(s) 408, 468
HpyAV CCTTC 2 cut(s) 296, 502
HpyCH4III ACNGT 2 cut(s) 296, 341
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 1 cut(s) 38
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 109, 118, 554
HpyF3I CTNAG 2 cut(s) 261, 531
HpySE526I ACGT 1 cut(s) 289
Hsp92II CATG 4 cut(s) 18, 25, 196, 275
HspAI GCGC 1 cut(s) 160
Kzo9I GATC 1 cut(s) 370
LweI GCATC 2 cut(s) 25, 296
MaeI CTAG 2 cut(s) 413, 504
MaeII ACGT 1 cut(s) 289
MaeIII GTNAC 1 cut(s) 290
MalI GATC 1 cut(s) 372
MboI GATC 1 cut(s) 370
MboII GAAGA 3 cut(s) 259, 293, 503
MhlI GDGCHC 2 cut(s) 114, 267
MluCI AATT 3 cut(s) 187, 221, 393
MmeI TCCRAC 2 cut(s) 323, 520
MnlI CCTC 5 cut(s) 4, 39, 63, 229, 435
MroXI GAANNNNTTC 1 cut(s) 251
MseI TTAA 2 cut(s) 360, 562
MslI CAYNNNNRTG 2 cut(s) 270, 276
MspI CCGG 4 cut(s) 215, 366, 390, 500
MspR9I CCNGG 2 cut(s) 178, 215
MvaI CCWGG 1 cut(s) 178
MwoI GCNNNNNNNGC 4 cut(s) 11, 109, 118, 554
NciI CCSGG 1 cut(s) 215
NdeII GATC 1 cut(s) 370
NlaIII CATG 4 cut(s) 18, 25, 196, 275
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 176
PspFI CCCAGC 1 cut(s) 103
PspGI CCWGG 1 cut(s) 176
RsaI GTAC 2 cut(s) 314, 515
RsaNI GTAC 2 cut(s) 313, 514
RseI CAYNNNNRTG 2 cut(s) 270, 276
SaqAI TTAA 2 cut(s) 360, 562
Sau3AI GATC 1 cut(s) 370
ScrFI CCNGG 2 cut(s) 178, 215
SduI GDGCHC 2 cut(s) 114, 267
SetI ASST 8 cut(s) 105, 246, 292, 388, 407, 446, 527, 559
SfaNI GCATC 2 cut(s) 25, 296
SmiMI CAYNNNNRTG 2 cut(s) 270, 276
Sse9I AATT 3 cut(s) 187, 221, 393
SsiI CCGC 2 cut(s) 309, 485
SspMI CTAG 2 cut(s) 413, 504
StyD4I CCNGG 2 cut(s) 176, 213
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 2 cut(s) 296, 341
TaiI ACGT 1 cut(s) 292
TaqI TCGA 1 cut(s) 481
TasI AATT 3 cut(s) 187, 221, 393
TatI WGTACW 1 cut(s) 513
TfiI GAWTC 1 cut(s) 351
Tru1I TTAA 2 cut(s) 360, 562
Tru9I TTAA 2 cut(s) 360, 562
TscAI CASTG 1 cut(s) 265
TspDTI ATGAA 4 cut(s) 10, 165, 260, 294
TspRI CASTG 1 cut(s) 265
XapI RAATTY 1 cut(s) 393
XmnI GAANNNNTTC 1 cut(s) 251
XspI CTAG 2 cut(s) 413, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.