Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0011324

Physical Location & Seq

Genomic Coordinates Reverse (-)

15255876 .. 15261202

Gene Structure 5327 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3087 bp

ATGGCTCCGACGCCGTCTTCTTCCAAATCAAGCCACTCCCACACAACCCAGTTCAAGACTCCGCTATCCAAGCACCGCCTCCAGTTCCCGAAAGTGCACCCATCTCCGAACCCAAACTCCGCCGTAAAAGATCCACCGGCGGAGCACCCGGTCGAAGTTCTAGCCCGAATCCGAGACCACCCAGATCGAAAAGAACAACCCGCATCGGTTCTCCAGATCAATCCTCAGAGGCAGAGTATTCGTGTCCGGGCCGATTTTGGGTACCGGGATTTCACGCTCGATGGGGTTTCGTTATCGGAAGAAGACGATCTAGGGACGTTTTACAGCAAATTTGTCCAGTCAAGGATTGACAGTGTGAAATTGGGAGAAAAATGTACGATCATGATGTACGGCCCAACTGGGTCGGGCAAGAGTCACACCATGTTCGGGTGTTGCAAGCAGCCCGGGATCGTTTACAGGTCGTTGAAAGATATTCTCGGGGATGGGGAAGATGACGGCGGCGCCGCGGTTGGGTCGTATGTGCAGGTCACTGTTTTGGAGATTTACAATGAGGAGATTTATGATTTGTTGCCGAGCAACAGCGGCGGGGGATTCGGGTATGGATGGCCAAAGGGCAATGGCTCCAATTCTAAGGTTAGGCTTGAAGTTATGGGGAAGAAGGCAAAGAATGCAACCTACATTTCTGGAAACGAAGCTGGGAAAATTTCAAAAGAGATTCAGAAAGTGGAGAAAAGAAGGATTGTTAAGAGCACCCTTTGCAACGATAGAAGTTCTCGAAGCCATTGCATGATAATCCTTGATGTCCCAACAGTGGGAGGATGCTTGATGCTCGTGGACATGGCTGGATCTGAAAATATTGAGCAAGCTGGCCAAATTGGGTTCGAGGCAAAAATGCAGACTGCAAAGATCAACCAGGGAAACATAGCTCTGAAAAGGGTGGTTGAATCCATCGCCAATGGTGATTCTCATGTGCCCTTCAGGGACAGCAAACTGACCATGCTTCTACAGGATTCTTTTGAGGACGACAAGACAAAAATCTTAATGGTCCTGTGTGCAAGTCCAGACCCAAAGGAGATACACAAGACAATCTCCACCCTTGAATATGGTGCAAAAGCAAAATGTATTGTCCGCGGACCTCATACACCAATCAAGGACAAAACTGGGACTGAAGATTCATCTGCAGCTATTTTAGGATCAAGGATTGCTGCCATGGATGAATTTATCCTAAAACTGCAAAGGGAGAATAAGCTCAAAGAGAAAGAACGGAATAAAGCACACAAGGAGCTAATGAGTAAAGAAGAAGAGGTTGCTGCATTGAGATCTAAACTTGAGCTTATGGAAGGGAAGGGATCAGGGGCAAAGGAGGAAGAAATCAACTTGAAGGTGAATGAAGTGGCCCAGGCTCTGAAAAATGAGTTGGAAAAGAAGTTGGCAGAATGCCAGAGAATGGCGAATGAGTTTGTTGAACTCGAGAGAAGGAGAATGGAAGAAAGGATATTGCAACAGCAGCAGGAAGTTGAAATGCTGAGGCGGCGGTTGGAGGAAATTGAGCTTGAACTGTGCCGCTCAAGCGATGGAAATGGTAAGGAAAGCGGGTCGAAGGAGCCTGATGGAATGCAGTTTGCCAAAAGGTTGTTAGGAATCTATGCCAGTTATGATGCAGGAGGAATGGTAACGTCAATGGATTTAGACATGGATGATCAAGAAACGTTTGCTCGGGAGATCAAATATGTAGGAGTGGTACCTGGGGTTGCTTATCCATCTGACAGCAATAATGTAAACACTGCACACATTGATTCTTTTGAGCCAAAATATGCTGACAGGGTATGCCTGAGCACTGTTTTTGAGGAAGAAGAAATGGAAGAAGAGGAAGGAGCTAACAAAAATGTGGAAGCTGAAGAGGTTGAGAAAGAAGTTATAGAGGAAAAGAGAGTATGTATGGTTGATGGATCTAGCTTCAGGAGTAATTATAATGTGGACTCCCTGACATCTTTACCTCAGGATTATCAAGATACACCAACTGTGCTTCGGCAAAGCAGAAATCCAGAGGAAGAAGATAACAATGGAAAACCAGATGATAGGCTTGTCAGAATCCGCAACATATTTACACTCTGTGGAGATTACAGAGAGATCTCTCAGCATATCCCACGTCCAGCACCTACTGTTGCATCAGTTAAATCAGAAAATGAGCAGAAACTGGCAGAACCAGAGACAAACAAAAGCCGAGTGTGTGATGAGACAGCATTGGCCTCCAAGGAAAACTACGACCCTTTAAATGAGGGCACTGACTCGGATACTGCAGAAGTGTATGTGAAATGGGAAGCTTCAAAAGAAAATCCAGGGAAGTTCATTACAACACTTAAGGTTGTAAAAGACGCAACCCTTGCTGAGCTCCGAAAGTTGATTGAAATCTATCTTGGTGCAGACAATCAAGCATTCACTTTTGTCATGCTTGGAGTTGGGGACCCTAGTGGAGCATCGGTAGCGAAGGAGAAGGAAGCAGCAACTCAGACAACAAAGCTTCCCCTACACAACAATCAATCAAATAGGTACCTGGCCTGCTTGAGACCACTGAAGGGAATGCATAGAATTGACCCTAGTGGAGCATCGGTAGCGAAGGAGAAGGAAGCAGCAACTCAGACAACAAAGCTTCCCCTACACAACAATCAATCAAATAGGTACCTGGCCTGCTTGAGACCACTGAAGGGAATGCAAAGCCCAAGTAGGCTTCTCCCATTAAGTCCCCTCCCATTGAAATTGCTGGAAAACAGGCTACCAGTCACTCCGACTTCCTGCTTCTCAGTCCAAGGTGGTGATTGTACGACACCCAAAGTAGCACCAAACTTAGGTTCTACTCCATATGTTACTGTCAGAAGACATTACACTGTCAGAGAGATGAAGGACAATATATTTTGCAAGGTTAGAGTGCCAGTTTGTGTTCAGAGTATTGTAGTTGCCGGTAAATGCCTTGATGATGAATGCTGTCTTTCAAGTTACAACATTCAGAAAGGCTCCACTCTCCACATGGTTTTGGGTTGGCGAACCAAATTCCCCTATCATTCATCATTCACAAGAAATTCAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005871 GO:0005875 GO:0006810 GO:0006928 GO:0007017 GO:0007018 GO:0008017 GO:0008092 GO:0008150 GO:0008574 GO:0009524 GO:0009987 GO:0010970 GO:0015630 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030705 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0047496 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0072384 GO:0099111 GO:0099518 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1029

Amino Acids

114.4

Weight (kDa)

6.29

Isoelectric Point (pI)

55.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	76 - 375	1.6e-59	Kinesin motor domain
Microtub_bd	PF16796	84 - 190	9.2e-09	Microtubule binding
ubiquitin	PF00240	955 - 1011	3e-10	Ubiquitin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011324)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14330
fragaria_vesca	FvH4_4g04000 FvH4_4g04000
malus_domestica	MD13G1216300.v1.1 MD16G1221700.v1.1
prunus_persica	Prupe.1G042100_v2.0.a1 Prupe.4G184200_v2.0.a1
pyrus_communis	pycom13g18980 pycom16g18590
rosa_chinensis	RchiOBHm_Chr4g0394251
rosa_laevigata	RLG00000009711
rosa_multiflora	Rmu_sc0007246.1_g000005 Rmu_ssc0000473.1_g000031
rosa_roxburghii	Rroxscaffold_5G00339300
rosa_rugosa	Rorug03G0368300.1
rosa_samantha	Rh4AG051100 Rh4BG048100 Rh4CG054800 Rh4DG047800
rosa_wichuraiana	Rw4G004070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1971
Acc36I	ACCTGC	1 cut(s)	514
Acc65I	GGTACC	4 cut(s)	261, 1741, 2550, 2679
AccB1I	GGYRCC	5 cut(s)	261, 500, 1741, 2550, 2679
AccB7I	CCANNNNNTGG	1 cut(s)	1447
AccBSI	CCGCTC	1 cut(s)	1566
AccII	CGCG	2 cut(s)	506, 1131
AclI	AACGTT	1 cut(s)	1709
AclWI	GGATC	6 cut(s)	125, 455, 853, 1201, 1357, 1957
AcoI	YGGCCR	2 cut(s)	605, 868
AcsI	RAATTY	5 cut(s)	329, 702, 1217, 3047, 3076
AcuI	CTGAAG	6 cut(s)	961, 1188, 1917, 1942, 2594, 2723
AcyI	GRCGYC	2 cut(s)	11, 501
AdeI	CACNNNGTG	1 cut(s)	2114
AfaI	GTAC	7 cut(s)	263, 376, 389, 1743, 2552, 2681, 2821
AfiI	CCNNNNNNNGG	9 cut(s)	258, 426, 510, 811, 812, 1447, 2576, 2705, 2846
AflII	CTTAAG	1 cut(s)	2360
AjiI	CACGTC	1 cut(s)	2150
AjnI	CCWGG	6 cut(s)	912, 1398, 1744, 2338, 2553, 2682
AjuI	GAANNNNNNNTTGG	6 cut(s)	1400, 1432, 2400, 2432, 2833, 2865
AloI	GAACNNNNNNTCC	2 cut(s)	101, 133
Alw21I	GWGCWC	5 cut(s)	99, 147, 752, 1838, 2394
Alw26I	GTCTC	5 cut(s)	168, 2204, 2231, 2560, 2689
Alw44I	GTGCAC	1 cut(s)	95
AlwI	GGATC	6 cut(s)	125, 455, 853, 1201, 1357, 1957
AlwNI	CAGNNNCTG	2 cut(s)	1405, 2197
Ama87I	CYCGRG	4 cut(s)	443, 476, 1469, 1716
AoxI	GGCC	8 cut(s)	249, 391, 605, 868, 1395, 2247, 2556, 2685
ApaLI	GTGCAC	1 cut(s)	95
ApeKI	GCWGC	7 cut(s)	439, 1181, 1205, 1310, 1507, 2501, 2630
ApoI	RAATTY	5 cut(s)	329, 702, 1217, 3047, 3076
Asp718I	GGTACC	4 cut(s)	261, 1741, 2550, 2679
AspLEI	GCGC	1 cut(s)	503
AspS9I	GGNCC	6 cut(s)	249, 392, 1045, 1133, 1396, 2464
AsuC2I	CCSGG	5 cut(s)	149, 248, 266, 444, 445
AsuHPI	GGTGA	3 cut(s)	971, 1396, 2825
AvaI	CYCGRG	4 cut(s)	443, 476, 1469, 1716
AvaII	GGWCC	3 cut(s)	1045, 1133, 2464
AxyI	CCTNAGG	1 cut(s)	1998
BaeGI	GKGCMC	3 cut(s)	99, 975, 2285
BalI	TGGCCA	2 cut(s)	607, 870
BanI	GGYRCC	5 cut(s)	261, 500, 1741, 2550, 2679
BanII	GRGCYC	1 cut(s)	2394
BauI	CACGAG	1 cut(s)	830
BbsI	GAAGAC	3 cut(s)	9, 309, 2881
Bbv12I	GWGCWC	5 cut(s)	99, 147, 752, 1838, 2394
BbvCI	CCTCAGC	1 cut(s)	1526
BbvI	GCAGC	7 cut(s)	451, 1192, 1193, 1297, 1519, 2513, 2642
BccI	CCATC	9 cut(s)	109, 275, 476, 597, 956, 1568, 1604, 1768, 1940
BceAI	ACGGC	3 cut(s)	107, 406, 511
BcgI	CGANNNNNNTGC	4 cut(s)	221, 255, 765, 799
BciT130I	CCWGG	6 cut(s)	914, 1400, 1746, 2340, 2555, 2684
BciVI	GTATCC	1 cut(s)	2287
BclI	TGATCA	1 cut(s)	1699
BcnI	CCSGG	5 cut(s)	149, 248, 266, 444, 445
BcoDI	GTCTC	5 cut(s)	168, 2204, 2231, 2560, 2689
BfaI	CTAG	5 cut(s)	161, 311, 1953, 2469, 2598
BfmI	CTRYAG	3 cut(s)	1004, 1179, 2298
BfoI	RGCGCY	1 cut(s)	504
BfrI	CTTAAG	1 cut(s)	2360
BfuAI	ACCTGC	1 cut(s)	514
BfuI	GTATCC	1 cut(s)	2287
BglII	AGATCT	2 cut(s)	1319, 2130
BlpI	GCTNAGC	1 cut(s)	2388
Bme18I	GGWCC	3 cut(s)	1045, 1133, 2464
BmeT110I	CYCGRG	4 cut(s)	443, 476, 1469, 1716
BmgBI	CACGTC	1 cut(s)	2150
BmgT120I	GGNCC	6 cut(s)	249, 392, 1045, 1133, 1396, 2464
BmrI	ACTGGG	3 cut(s)	43, 408, 1170
BmsI	GCATC	7 cut(s)	212, 809, 816, 1648, 2177, 2486, 2615
BmuI	ACTGGG	3 cut(s)	43, 408, 1170
BpiI	GAAGAC	3 cut(s)	9, 309, 2881
BpmI	CTGGAG	2 cut(s)	65, 197
Bpu10I	CCTNAGC	2 cut(s)	1526, 1832
Bpu1102I	GCTNAGC	1 cut(s)	2388
BpuEI	CTTGAG	4 cut(s)	1349, 1552, 2584, 2713
BpuMI	CCSGG	5 cut(s)	149, 248, 266, 444, 445
BsaBI	GATNNNNATC	1 cut(s)	2408
BsaHI	GRCGYC	2 cut(s)	11, 501
BsaI	GGTCTC	3 cut(s)	168, 2560, 2689
BsaXI	ACNNNNNCTCC	8 cut(s)	101, 131, 1656, 1686, 2466, 2496, 2595, 2625
Bsc4I	CCNNNNNNNGG	9 cut(s)	258, 426, 510, 811, 812, 1447, 2576, 2705, 2846
Bse118I	RCCGGY	2 cut(s)	136, 2957
Bse1I	ACTGG	9 cut(s)	49, 82, 337, 403, 1165, 1650, 2202, 2777, 2930
Bse21I	CCTNAGG	1 cut(s)	1998
Bse3DI	GCAATG	2 cut(s)	622, 781
Bse8I	GATNNNNATC	1 cut(s)	2408
BseBI	CCWGG	6 cut(s)	914, 1400, 1746, 2340, 2555, 2684
BseGI	GGATG	5 cut(s)	487, 608, 824, 1219, 1702
BseJI	GATNNNNATC	1 cut(s)	2408
BseLI	CCNNNNNNNGG	9 cut(s)	258, 426, 510, 811, 812, 1447, 2576, 2705, 2846
BseMI	GCAATG	2 cut(s)	622, 781
BseMII	CTCAG	9 cut(s)	239, 1517, 1823, 2012, 2150, 2379, 2522, 2651, 2814
BseNI	ACTGG	9 cut(s)	49, 82, 337, 403, 1165, 1650, 2202, 2777, 2930
BseRI	GAGGAG	1 cut(s)	566
BseSI	GKGCMC	3 cut(s)	99, 975, 2285
BseXI	GCAGC	7 cut(s)	451, 1192, 1193, 1297, 1519, 2513, 2642
BseYI	CCCAGC	1 cut(s)	695
BsgI	GTGCAG	3 cut(s)	542, 1770, 2442
Bsh1236I	CGCG	2 cut(s)	506, 1131
Bsh1285I	CGRYCG	1 cut(s)	153
BshFI	GGCC	8 cut(s)	251, 393, 607, 870, 1397, 2249, 2558, 2687
BshNI	GGYRCC	5 cut(s)	261, 500, 1741, 2550, 2679
BsiEI	CGRYCG	1 cut(s)	153
BsiHKAI	GWGCWC	5 cut(s)	99, 147, 752, 1838, 2394
BsiHKCI	CYCGRG	4 cut(s)	443, 476, 1469, 1716
BsiSI	CCGG	6 cut(s)	137, 149, 247, 265, 444, 2958
BslFI	GGGAC	6 cut(s)	328, 788, 995, 1177, 2477, 2727
BslI	CCNNNNNNNGG	9 cut(s)	258, 426, 510, 811, 812, 1447, 2576, 2705, 2846
BsmAI	GTCTC	5 cut(s)	168, 2204, 2231, 2560, 2689
BsmFI	GGGAC	6 cut(s)	328, 788, 995, 1177, 2477, 2727
BsmI	GAATGC	7 cut(s)	673, 1442, 1620, 2435, 2586, 2715, 2984
BsnI	GGCC	8 cut(s)	251, 393, 607, 870, 1397, 2249, 2558, 2687
Bso31I	GGTCTC	3 cut(s)	168, 2560, 2689
BsoBI	CYCGRG	4 cut(s)	443, 476, 1469, 1716
Bsp1286I	GDGCHC	7 cut(s)	99, 147, 752, 975, 1838, 2285, 2394
Bsp1720I	GCTNAGC	1 cut(s)	2388
Bsp19I	CCATGG	1 cut(s)	1209
BspANI	GGCC	8 cut(s)	251, 393, 607, 870, 1397, 2249, 2558, 2687
BspCNI	CTCAG	9 cut(s)	238, 1518, 1824, 2011, 2149, 2380, 2521, 2650, 2813
BspFNI	CGCG	2 cut(s)	506, 1131
BspHI	TCATGA	1 cut(s)	381
BspMAI	CTGCAG	2 cut(s)	1183, 2302
BspMI	ACCTGC	1 cut(s)	514
BspPI	GGATC	6 cut(s)	125, 455, 853, 1201, 1357, 1957
BspT107I	GGYRCC	5 cut(s)	261, 500, 1741, 2550, 2679
BspTI	CTTAAG	1 cut(s)	2360
BspTNI	GGTCTC	3 cut(s)	168, 2560, 2689
BsrBI	CCGCTC	1 cut(s)	1566
BsrDI	GCAATG	2 cut(s)	622, 781
BsrFI	RCCGGY	2 cut(s)	136, 2957
BsrI	ACTGG	9 cut(s)	49, 82, 337, 403, 1165, 1650, 2202, 2777, 2930
BssAI	RCCGGY	2 cut(s)	136, 2957
BssNI	GRCGYC	2 cut(s)	11, 501
BssSI	CACGAG	1 cut(s)	830
BssT1I	CCWWGG	3 cut(s)	1209, 2253, 2806
Bst2BI	CACGAG	1 cut(s)	830
Bst2UI	CCWGG	6 cut(s)	914, 1400, 1746, 2340, 2555, 2684
Bst4CI	ACNGT	9 cut(s)	353, 532, 811, 1560, 1840, 2023, 2164, 2869, 2887
Bst6I	CTCTTC	3 cut(s)	1296, 1860, 1893
BstACI	GRCGYC	2 cut(s)	11, 501
BstAFI	CTTAAG	1 cut(s)	2360
BstAPI	GCANNNNNTGC	3 cut(s)	668, 756, 2384
BstC8I	GCNNGC	5 cut(s)	437, 864, 868, 2560, 2689
BstDSI	CCRYGG	3 cut(s)	504, 1129, 1209
BstF5I	GGATG	5 cut(s)	487, 608, 824, 1219, 1702
BstFNI	CGCG	2 cut(s)	506, 1131
BstH2I	RGCGCY	1 cut(s)	504
BstHHI	GCGC	1 cut(s)	503
BstMAI	GTCTC	5 cut(s)	168, 2204, 2231, 2560, 2689
BstMCI	CGRYCG	1 cut(s)	153
BstNI	CCWGG	6 cut(s)	914, 1400, 1746, 2340, 2555, 2684
BstSFI	CTRYAG	3 cut(s)	1004, 1179, 2298
BstSLI	GKGCMC	3 cut(s)	99, 975, 2285
BstUI	CGCG	2 cut(s)	506, 1131
BstV1I	GCAGC	7 cut(s)	451, 1192, 1193, 1297, 1519, 2513, 2642
BstV2I	GAAGAC	3 cut(s)	9, 309, 2881
BstX2I	RGATCY	5 cut(s)	130, 845, 1319, 1949, 2130
BstYI	RGATCY	5 cut(s)	130, 845, 1319, 1949, 2130
Bsu36I	CCTNAGG	1 cut(s)	1998
BsuI	GTATCC	1 cut(s)	2287
BsuRI	GGCC	8 cut(s)	251, 393, 607, 870, 1397, 2249, 2558, 2687
BtgI	CCRYGG	3 cut(s)	504, 1129, 1209
BtgZI	GCGATG	2 cut(s)	934, 1587
BtrI	CACGTC	1 cut(s)	2150
BtsCI	GGATG	5 cut(s)	487, 608, 824, 1219, 1702
BtsI	GCAGTG	1 cut(s)	1782
BtsIMutI	CAGTG	9 cut(s)	358, 528, 816, 1782, 1836, 2283, 2570, 2699, 2883
BveI	ACCTGC	1 cut(s)	514
Cac8I	GCNNGC	5 cut(s)	437, 864, 868, 2560, 2689
CaiI	CAGNNNCTG	2 cut(s)	1405, 2197
CciI	TCATGA	1 cut(s)	381
CfoI	GCGC	1 cut(s)	503
Cfr10I	RCCGGY	2 cut(s)	136, 2957
Cfr13I	GGNCC	6 cut(s)	249, 392, 1045, 1133, 1396, 2464
Cfr42I	CCGCGG	2 cut(s)	507, 1132
Cfr9I	CCCGGG	1 cut(s)	443
CseI	GACGC	2 cut(s)	19, 2384
Csp6I	GTAC	7 cut(s)	262, 375, 388, 1742, 2551, 2680, 2820
CviQI	GTAC	7 cut(s)	262, 375, 388, 1742, 2551, 2680, 2820
DinI	GGCGCC	1 cut(s)	502
DraI	TTTAAA	1 cut(s)	2274
DraIII	CACNNNGTG	1 cut(s)	2114
EaeI	YGGCCR	2 cut(s)	605, 868
Eam1104I	CTCTTC	3 cut(s)	1296, 1860, 1893
EarI	CTCTTC	3 cut(s)	1296, 1860, 1893
EciI	GGCGGA	2 cut(s)	109, 155
Ecl136II	GAGCTC	1 cut(s)	2392
Eco130I	CCWWGG	3 cut(s)	1209, 2253, 2806
Eco24I	GRGCYC	1 cut(s)	2394
Eco31I	GGTCTC	3 cut(s)	168, 2560, 2689
Eco47I	GGWCC	3 cut(s)	1045, 1133, 2464
Eco53kI	GAGCTC	1 cut(s)	2392
Eco57I	CTGAAG	6 cut(s)	961, 1188, 1917, 1942, 2594, 2723
Eco81I	CCTNAGG	1 cut(s)	1998
Eco88I	CYCGRG	4 cut(s)	443, 476, 1469, 1716
EcoICRI	GAGCTC	1 cut(s)	2392
EcoO109I	RGGNCCY	1 cut(s)	2464
EcoRII	CCWGG	6 cut(s)	912, 1398, 1744, 2338, 2553, 2682
EcoT14I	CCWWGG	3 cut(s)	1209, 2253, 2806
EcoT22I	ATGCAT	1 cut(s)	2586
EcoT38I	GRGCYC	1 cut(s)	2394
EgeI	GGCGCC	1 cut(s)	502
EheI	GGCGCC	1 cut(s)	502
ErhI	CCWWGG	3 cut(s)	1209, 2253, 2806
FaqI	GGGAC	6 cut(s)	328, 788, 995, 1177, 2477, 2727
FauI	CCCGC	3 cut(s)	208, 578, 1586
FauNDI	CATATG	1 cut(s)	2860
FbaI	TGATCA	1 cut(s)	1699
FokI	GGATG	5 cut(s)	494, 615, 831, 1226, 1709
FriOI	GRGCYC	1 cut(s)	2394
FspBI	CTAG	5 cut(s)	161, 311, 1953, 2469, 2598
GlaI	GCGC	1 cut(s)	502
GsaI	CCCAGC	1 cut(s)	699
GsuI	CTGGAG	2 cut(s)	65, 197
HaeII	RGCGCY	1 cut(s)	504
HaeIII	GGCC	8 cut(s)	251, 393, 607, 870, 1397, 2249, 2558, 2687
HapII	CCGG	6 cut(s)	137, 149, 247, 265, 444, 2958
HgaI	GACGC	2 cut(s)	19, 2384
HhaI	GCGC	1 cut(s)	503
Hin1I	GRCGYC	2 cut(s)	11, 501
Hin6I	GCGC	1 cut(s)	501
HinP1I	GCGC	1 cut(s)	501
HindIII	AAGCTT	3 cut(s)	2322, 2519, 2648
HpaII	CCGG	6 cut(s)	137, 149, 247, 265, 444, 2958
HphI	GGTGA	3 cut(s)	971, 1396, 2825
Hpy166II	GTNNAC	5 cut(s)	97, 454, 835, 1780, 1978
Hpy8I	GTNNAC	5 cut(s)	97, 454, 835, 1780, 1978
Hpy99I	CGWCG	1 cut(s)	13
HpyCH4III	ACNGT	9 cut(s)	353, 532, 811, 1560, 1840, 2023, 2164, 2869, 2887
HpyCH4IV	ACGT	4 cut(s)	317, 1676, 1709, 2149
HpySE526I	ACGT	4 cut(s)	317, 1676, 1709, 2149
Hsp92I	GRCGYC	2 cut(s)	11, 501
HspAI	GCGC	1 cut(s)	501
KasI	GGCGCC	1 cut(s)	500
KflI	GGGWCCC	1 cut(s)	2464
KpnI	GGTACC	4 cut(s)	265, 1745, 2554, 2683
Ksp22I	TGATCA	1 cut(s)	1699
KspI	CCGCGG	2 cut(s)	507, 1132
Lsp1109I	GCAGC	7 cut(s)	451, 1192, 1193, 1297, 1519, 2513, 2642
LweI	GCATC	7 cut(s)	212, 809, 816, 1648, 2177, 2486, 2615
MaeI	CTAG	5 cut(s)	161, 311, 1953, 2469, 2598
MaeII	ACGT	4 cut(s)	317, 1676, 1709, 2149
MaeIII	GTNAC	6 cut(s)	413, 526, 1672, 2779, 2863, 2993
MbiI	CCGCTC	1 cut(s)	1566
MflI	RGATCY	5 cut(s)	130, 845, 1319, 1949, 2130
MhlI	GDGCHC	7 cut(s)	99, 147, 752, 975, 1838, 2285, 2394
MlsI	TGGCCA	2 cut(s)	607, 870
MluNI	TGGCCA	2 cut(s)	607, 870
Mly113I	GGCGCC	1 cut(s)	501
MlyI	GAGTC	4 cut(s)	52, 421, 1973, 2282
MmeI	TCCRAC	4 cut(s)	32, 1398, 1518, 2810
Mox20I	TGGCCA	2 cut(s)	607, 870
Mph1103I	ATGCAT	1 cut(s)	2586
MscI	TGGCCA	2 cut(s)	607, 870
MseI	TTAA	6 cut(s)	744, 1040, 2175, 2273, 2361, 2738
Msp20I	TGGCCA	2 cut(s)	607, 870
MspA1I	CMGCKG	3 cut(s)	506, 582, 1131
MspCI	CTTAAG	1 cut(s)	2360
MspI	CCGG	6 cut(s)	137, 149, 247, 265, 444, 2958
Mva1269I	GAATGC	7 cut(s)	673, 1442, 1620, 2435, 2586, 2715, 2984
MvaI	CCWGG	6 cut(s)	914, 1400, 1746, 2340, 2555, 2684
MvnI	CGCG	2 cut(s)	506, 1131
NarI	GGCGCC	1 cut(s)	501
NciI	CCSGG	5 cut(s)	149, 248, 266, 444, 445
NcoI	CCATGG	1 cut(s)	1209
NdeI	CATATG	1 cut(s)	2860
NmeAIII	GCCGAG	2 cut(s)	597, 2249
NmuCI	GTSAC	3 cut(s)	413, 526, 2779
NsiI	ATGCAT	1 cut(s)	2586
PaeR7I	CTCGAG	1 cut(s)	1469
PagI	TCATGA	1 cut(s)	381
PctI	GAATGC	7 cut(s)	673, 1442, 1620, 2435, 2586, 2715, 2984
PflFI	GACNNNGTC	1 cut(s)	13
PflMI	CCANNNNNTGG	1 cut(s)	1447
PleI	GAGTC	4 cut(s)	52, 420, 1973, 2282
PluTI	GGCGCC	1 cut(s)	504
PpsI	GAGTC	4 cut(s)	52, 420, 1973, 2282
PpuMI	RGGWCCY	1 cut(s)	2464
PsiI	TTATAA	1 cut(s)	1971
Psp124BI	GAGCTC	1 cut(s)	2394
Psp1406I	AACGTT	1 cut(s)	1709
Psp5II	RGGWCCY	1 cut(s)	2464
Psp6I	CCWGG	6 cut(s)	912, 1398, 1744, 2338, 2553, 2682
PspFI	CCCAGC	1 cut(s)	695
PspGI	CCWGG	6 cut(s)	912, 1398, 1744, 2338, 2553, 2682
PspPI	GGNCC	6 cut(s)	249, 392, 1045, 1133, 1396, 2464
PspPPI	RGGWCCY	1 cut(s)	2464
PstI	CTGCAG	2 cut(s)	1183, 2302
PstNI	CAGNNNCTG	2 cut(s)	1405, 2197
PsuI	RGATCY	5 cut(s)	130, 845, 1319, 1949, 2130
PsyI	GACNNNGTC	1 cut(s)	13
RsaI	GTAC	7 cut(s)	263, 376, 389, 1743, 2552, 2681, 2821
RsaNI	GTAC	7 cut(s)	262, 375, 388, 1742, 2551, 2680, 2820
SacI	GAGCTC	1 cut(s)	2394
SacII	CCGCGG	2 cut(s)	507, 1132
SaqAI	TTAA	6 cut(s)	744, 1040, 2175, 2273, 2361, 2738
Sau96I	GGNCC	6 cut(s)	249, 392, 1045, 1133, 1396, 2464
SchI	GAGTC	4 cut(s)	52, 421, 1973, 2282
SduI	GDGCHC	7 cut(s)	99, 147, 752, 975, 1838, 2285, 2394
SfaNI	GCATC	7 cut(s)	212, 809, 816, 1648, 2177, 2486, 2615
SfcI	CTRYAG	3 cut(s)	1004, 1179, 2298
SfoI	GGCGCC	1 cut(s)	502
Sfr274I	CTCGAG	1 cut(s)	1469
Sfr303I	CCGCGG	2 cut(s)	507, 1132
SgrAI	CRCCGGYG	1 cut(s)	136
SgrBI	CCGCGG	2 cut(s)	507, 1132
SinI	GGWCC	3 cut(s)	1045, 1133, 2464
SlaI	CTCGAG	1 cut(s)	1469
SmaI	CCCGGG	1 cut(s)	445
SmlI	CTYRAG	6 cut(s)	1328, 1469, 1567, 2360, 2563, 2692
SmoI	CTYRAG	6 cut(s)	1328, 1469, 1567, 2360, 2563, 2692
SspDI	GGCGCC	1 cut(s)	500
SspI	AATATT	1 cut(s)	856
SspMI	CTAG	5 cut(s)	161, 311, 1953, 2469, 2598
SstI	GAGCTC	1 cut(s)	2394
StyI	CCWWGG	3 cut(s)	1209, 2253, 2806
TaaI	ACNGT	9 cut(s)	353, 532, 811, 1560, 1840, 2023, 2164, 2869, 2887
TaiI	ACGT	4 cut(s)	320, 1679, 1712, 2152
TaqI	TCGA	7 cut(s)	153, 187, 279, 775, 882, 1470, 1598
TauI	GCSGC	5 cut(s)	501, 506, 585, 1534, 1566
Tru1I	TTAA	6 cut(s)	744, 1040, 2175, 2273, 2361, 2738
Tru9I	TTAA	6 cut(s)	744, 1040, 2175, 2273, 2361, 2738
TscAI	CASTG	9 cut(s)	358, 535, 816, 1789, 1843, 2290, 2577, 2706, 2890
TseFI	GTSAC	3 cut(s)	413, 526, 2779
TseI	GCWGC	7 cut(s)	439, 1181, 1205, 1310, 1507, 2501, 2630
Tsp45I	GTSAC	3 cut(s)	413, 526, 2779
TspDTI	ATGAA	7 cut(s)	1164, 1230, 1404, 2338, 2912, 2991, 3051
TspGWI	ACGGA	1 cut(s)	1279
TspMI	CCCGGG	1 cut(s)	443
TspRI	CASTG	9 cut(s)	358, 535, 816, 1789, 1843, 2290, 2577, 2706, 2890
Tth111I	GACNNNGTC	1 cut(s)	13
Van91I	CCANNNNNTGG	1 cut(s)	1447
Vha464I	CTTAAG	1 cut(s)	2360
VneI	GTGCAC	1 cut(s)	95
VpaK11BI	GGWCC	3 cut(s)	1045, 1133, 2464
XapI	RAATTY	5 cut(s)	329, 702, 1217, 3047, 3076
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3022
XhoI	CTCGAG	1 cut(s)	1469
XmaI	CCCGGG	1 cut(s)	443
XspI	CTAG	5 cut(s)	161, 311, 1953, 2469, 2598
Zsp2I	ATGCAT	1 cut(s)	2586

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.