Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0013293

Physical Location & Seq

Genomic Coordinates Forward (+)

207879 .. 210356

Gene Structure 2478 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1959 bp

ATGACTCTACTCCTAATTCGGCGGATGCATTTACACCGGTCCCAAGCGCAGGCCATTAACAACCTCAACCTCACAACCTGGTTCAACAAATACGTCGACAAAGACGATGTCGCTTCCTGGAACTCCGTCATTGCCGACTTGGCCCGGAGCGGCGACTCCCTCGAAGCCCTCCGGGCATTTTCGTCCATGCGAAAGCTTTCCCTCAGACCAAACCGCTCCTCCTTCCCCTGCGCCATCAAATCCTGCTCGGCTTTGCTGGACCTTCACTCCGGCAAGCAAGCTCACCAGCAGGCGTTGGTCTTCGGCTTTGAGTCCGATCTCTTTGTCTCCTCTGCCCTTATCGATATGTACTCCAGATGCGGTCGATTGACGGATGCATGGAAACTGTTCGATACAATTCCTCACAGGAACGTGGTTACTTGGACGTCAATGATCACCGGGTGCGTCCAGAACGACGACGCCTGCCAAGCCTTGTCTCTCTTTAAAGAGTTGTTGATCGAGGAAATTGACAATGAGGATGTTTCTTTAGATCCTGTTGTTCTGGTTTCTGTTCTGTCGGCTTGTTCTCGAGTGTCAAGTAAGGGACTTGCTCAAACTGTTCATGGGTTGGTGGTGAAGAGAGGCTTTGATGGGTATTTATGGGTTGGGAATACTTTGATGGATGCATATGCCAAATGCGGCGAGCTCGAGCTTGTTAGAAAGGTGTTTGATGGAATGTCTCAAAAGGATTTGGTTTCTTGGAACTCCATGATTGCAACTTATGCACAGAGTGGATTATCTTCTGAGGCATGGCAAGTCTTCTACGGAATGGCAAGGGATGGTGACGTCCACTACAATGCCGTGACGTTGTCCACTTTGTTATTAGCTTGTGCACATGCTGGGCCTCTGCTAGTTGGGAAGTCTATACATGATCAGGTTCTGAAAAAGGGCTTGGAAGAGAATGTGATAGTGTCTACCTCTATTATCGACATGTACTGCAAACGTGGGAGAGTTGACTTGGCAAGGAAGGCATTTGATTGCATGAAGGAGAAGAATGTCAGGACATGGACTGCCATGGTTGCAGGTTATGGAATGCATGGCCGTGCCAAAGAAGCGTTGGAGGTCTTCTATAAGATGATAAGAGATGGTGTGACACCAAATTACATAACATTTGTGTCACTTCTAAATGCTTGCAGCCATGCTGGTCTATTAGAACAAGGTTGGTATTGGTTTAATGCCATGAGCCGTGAATTCAGTGTAGAACCTGGAATTGAGCACTATGGGTGCATGGTTGATCTTCTTGGACGTTCTGGTGATCTTAACAAGGCTTATGATTTAATAAAGGGAATGAGGATCATGCCAGATTGTGTTGTCTGGGGTTCATTTCTTGGGGCTTGCAGGGTACACAAAAATGTGAGATTTGCAGAGATTTCTGCAAGAAAACTGTTTGAGCTTGACCCTCATAATTGTGGGTACTACATCTTGCTTTCAAATATATATGCCGATGCTGGGAGATGGGAAGATGTTGAGAGGATGAGAGTACTCATGAAGAATCGTGGGTTGGTAAAACCTCCTGGATTCAGTTTGGTTGAACTGAGAGGTATTGTGCATTTATTTTTAGTTGGAGACAGAGAGCATCCTCAGCATGAGAAGATTTATGAGTACTTGGAAAAACTGACAATCAAGCTGCAAGAGGTTGGCTATGCACCCAATATCAGTTCAGTTCTTCATGACGTTGATGAGGAAGAGAAGGAAATGGTCCTAAGAGTACATAGTGAGAAGCTGGCCGTTGCCTTTGGAATCATGAATTCTGTACCTGGTGCGACGATTCAGATAATCAAGAATCTCCGAGTTTGTGCCAATTGTCATACTGTTATTAAGTTGATTACGAAGGTTGTTGATCGGGAAATTGTAGTCAGAGATTCAAAGCGGTTTCATCACTTCAGGAATGGGTTGTGTTCTTGTGGAGATTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

653

Amino Acids

73.44

Weight (kDa)

7.8

Isoelectric Point (pI)

39.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	243 - 292	5.2e-06	PPR repeat family
PPR_2	PF13041	344 - 392	5.3e-10	PPR repeat family
PPR_3	PF13812	345 - 391	1.7e-06	Pentatricopeptide repeat domain
PPR	PF01535	348 - 377	8.5e-08	PPR repeat
E_motif	PF20431	462 - 524	4.7e-21	E motif
DYW_deaminase	PF14432	560 - 652	2.3e-42	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013293)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26782
fragaria_vesca	FvH4_6g54200 FvH4_6g54200 FvH4_6g54200
malus_domestica	MD09G1004300.v1.1 MD17G1007800.v1.1
prunus_persica	Prupe.3G312400_v2.0.a1
pyrus_communis	pycom111g00310 pycom17g00330
rosa_chinensis	RchiOBHm_Chr2g0176381
rosa_laevigata	RLG00000022408
rosa_multiflora	Rmu_co8497233.1_g000001 Rmu_sc0009140.1_g000003
rosa_roxburghii	Rroxscaffold_2G00076480
rosa_rugosa	Rorug02G0593800
rosa_samantha	Rh2BG683700 Rh2CG646400
rosa_wichuraiana	Rw2G055130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	428, 828
Acc36I	ACCTGC	1 cut(s)	1052
AccBSI	CCGCTC	2 cut(s)	150, 216
AccI	GTMKAC	2 cut(s)	96, 953
AciI	CCGC	6 cut(s)	22, 150, 214, 360, 678, 1909
AclWI	GGATC	2 cut(s)	524, 1340
AcoI	YGGCCR	2 cut(s)	1078, 1764
AcsI	RAATTY	2 cut(s)	1229, 1786
AcuI	CTGAAG	1 cut(s)	1906
AcyI	GRCGYC	3 cut(s)	425, 459, 825
AdeI	CACNNNGTG	2 cut(s)	441, 770
AfaI	GTAC	8 cut(s)	350, 974, 1383, 1454, 1521, 1643, 1749, 1794
AfiI	CCNNNNNNNGG	2 cut(s)	49, 1488
AflIII	ACRYGT	1 cut(s)	969
AgeI	ACCGGT	1 cut(s)	36
AgsI	TTSAA	4 cut(s)	85, 1470, 1571, 1905
AjnI	CCWGG	5 cut(s)	77, 116, 1243, 1552, 1795
AjuI	GAANNNNNNNTTGG	4 cut(s)	914, 946, 1523, 1555
AluBI	AGCT	8 cut(s)	196, 281, 685, 691, 866, 1432, 1666, 1762
AluI	AGCT	8 cut(s)	196, 281, 685, 691, 866, 1432, 1666, 1762
Alw21I	GWGCWC	3 cut(s)	687, 874, 1257
Alw26I	GTCTC	4 cut(s)	331, 480, 723, 1599
Alw44I	GTGCAC	1 cut(s)	870
AlwI	GGATC	2 cut(s)	524, 1340
AlwNI	CAGNNNCTG	1 cut(s)	919
Ama87I	CYCGRG	2 cut(s)	567, 686
AoxI	GGCC	5 cut(s)	51, 141, 881, 1078, 1764
ApaLI	GTGCAC	1 cut(s)	870
ApeKI	GCWGC	2 cut(s)	1173, 1666
ApoI	RAATTY	2 cut(s)	1229, 1786
ArsI	GACNNNNNNTTYG	2 cut(s)	93, 125
AsiGI	ACCGGT	1 cut(s)	36
Asp700I	GAANNNNTTC	2 cut(s)	196, 386
AspLEI	GCGC	2 cut(s)	49, 233
AspS9I	GGNCC	5 cut(s)	39, 142, 259, 881, 1738
AsuC2I	CCSGG	3 cut(s)	145, 173, 439
AsuHPI	GGTGA	5 cut(s)	275, 427, 625, 833, 1304
AvaI	CYCGRG	2 cut(s)	567, 686
AvaII	GGWCC	3 cut(s)	39, 259, 1738
BaeGI	GKGCMC	1 cut(s)	874
BanII	GRGCYC	1 cut(s)	687
BbsI	GAAGAC	3 cut(s)	292, 790, 1096
Bbv12I	GWGCWC	3 cut(s)	687, 874, 1257
BbvCI	CCTCAGC	1 cut(s)	1620
BbvI	GCAGC	2 cut(s)	1185, 1653
BccI	CCATC	7 cut(s)	242, 623, 652, 704, 812, 1118, 1488
BceAI	ACGGC	4 cut(s)	824, 1065, 1209, 1751
BciT130I	CCWGG	5 cut(s)	79, 118, 1245, 1554, 1797
BclI	TGATCA	2 cut(s)	432, 910
BcnI	CCSGG	3 cut(s)	145, 173, 439
BcoDI	GTCTC	4 cut(s)	331, 480, 723, 1599
BfaI	CTAG	1 cut(s)	890
BfuAI	ACCTGC	1 cut(s)	1052
BglI	GCCNNNNNGGC	2 cut(s)	140, 173
BisI	GCNGC	4 cut(s)	151, 679, 1174, 1667
BlsI	GCNGC	4 cut(s)	152, 680, 1175, 1668
BmcAI	AGTACT	2 cut(s)	1521, 1643
Bme1390I	CCNGG	8 cut(s)	79, 118, 145, 173, 439, 1245, 1554, 1797
Bme18I	GGWCC	3 cut(s)	39, 259, 1738
BmeT110I	CYCGRG	2 cut(s)	567, 686
BmgT120I	GGNCC	5 cut(s)	39, 142, 259, 881, 1738
BmiI	GGNNCC	1 cut(s)	41
BmrFI	CCNGG	8 cut(s)	79, 118, 145, 173, 439, 1245, 1554, 1797
BmsI	GCATC	6 cut(s)	15, 347, 364, 652, 1474, 1624
BpiI	GAAGAC	3 cut(s)	292, 790, 1096
BpmI	CTGGAG	1 cut(s)	337
Bpu10I	CCTNAGC	1 cut(s)	1620
BpuMI	CCSGG	3 cut(s)	145, 173, 439
Bsa29I	ATCGAT	1 cut(s)	342
BsaHI	GRCGYC	3 cut(s)	425, 459, 825
BsaJI	CCNNGG	1 cut(s)	1053
BsaWI	WCCGGW	1 cut(s)	36
BsaXI	ACNNNNNCTCC	4 cut(s)	203, 233, 251, 281
Bsc4I	CCNNNNNNNGG	2 cut(s)	49, 1488
Bse118I	RCCGGY	1 cut(s)	36
Bse3DI	GCAATG	1 cut(s)	129
BseBI	CCWGG	5 cut(s)	79, 118, 1245, 1554, 1797
BseCI	ATCGAT	1 cut(s)	342
BseDI	CCNNGG	1 cut(s)	1053
BseGI	GGATG	7 cut(s)	30, 379, 523, 667, 823, 1518, 1615
BseLI	CCNNNNNNNGG	2 cut(s)	49, 1488
BseMI	GCAATG	1 cut(s)	129
BseMII	CTCAG	4 cut(s)	217, 774, 1565, 1634
BseRI	GAGGAG	2 cut(s)	208, 319
BseSI	GKGCMC	1 cut(s)	874
BseXI	GCAGC	2 cut(s)	1185, 1653
BseYI	CCCAGC	2 cut(s)	878, 1487
Bsh1285I	CGRYCG	1 cut(s)	364
BshFI	GGCC	5 cut(s)	53, 143, 883, 1080, 1766
BshTI	ACCGGT	1 cut(s)	36
BshVI	ATCGAT	1 cut(s)	342
BsiEI	CGRYCG	1 cut(s)	364
BsiHKAI	GWGCWC	3 cut(s)	687, 874, 1257
BsiHKCI	CYCGRG	2 cut(s)	567, 686
BsiSI	CCGG	5 cut(s)	37, 145, 172, 270, 438
BslFI	GGGAC	2 cut(s)	25, 597
BslI	CCNNNNNNNGG	2 cut(s)	49, 1488
BsmAI	GTCTC	4 cut(s)	331, 480, 723, 1599
BsmFI	GGGAC	2 cut(s)	25, 597
BsmI	GAATGC	1 cut(s)	1077
BsnI	GGCC	5 cut(s)	53, 143, 883, 1080, 1766
BsoBI	CYCGRG	2 cut(s)	567, 686
Bsp1286I	GDGCHC	3 cut(s)	687, 874, 1257
Bsp143I	GATC	9 cut(s)	316, 432, 495, 529, 910, 1273, 1294, 1332, 1879
Bsp19I	CCATGG	1 cut(s)	1053
BspACI	CCGC	6 cut(s)	22, 150, 214, 360, 678, 1909
BspANI	GGCC	5 cut(s)	53, 143, 883, 1080, 1766
BspCNI	CTCAG	4 cut(s)	216, 775, 1566, 1633
BspDI	ATCGAT	1 cut(s)	342
BspHI	TCATGA	3 cut(s)	1524, 1708, 1782
BspLI	GGNNCC	1 cut(s)	41
BspMI	ACCTGC	1 cut(s)	1052
BspPI	GGATC	2 cut(s)	524, 1340
BsrBI	CCGCTC	2 cut(s)	150, 216
BsrDI	GCAATG	1 cut(s)	129
BsrFI	RCCGGY	1 cut(s)	36
BssAI	RCCGGY	1 cut(s)	36
BssECI	CCNNGG	1 cut(s)	1053
BssMI	GATC	9 cut(s)	316, 432, 495, 529, 910, 1273, 1294, 1332, 1879
BssNI	GRCGYC	3 cut(s)	425, 459, 825
BssT1I	CCWWGG	1 cut(s)	1053
Bst2UI	CCWGG	5 cut(s)	79, 118, 1245, 1554, 1797
Bst4CI	ACNGT	4 cut(s)	387, 598, 1425, 1852
Bst6I	CTCTTC	3 cut(s)	611, 930, 1719
BstACI	GRCGYC	3 cut(s)	425, 459, 825
BstAPI	GCANNNNNTGC	1 cut(s)	761
BstC8I	GCNNGC	9 cut(s)	51, 275, 279, 291, 463, 683, 1171, 1375, 1764
BstDEI	CTNAG	5 cut(s)	203, 783, 1574, 1620, 1742
BstDSI	CCRYGG	1 cut(s)	1053
BstF5I	GGATG	7 cut(s)	30, 379, 523, 667, 823, 1518, 1615
BstHHI	GCGC	2 cut(s)	49, 233
BstKTI	GATC	9 cut(s)	319, 435, 498, 532, 913, 1276, 1297, 1335, 1882
BstMAI	GTCTC	4 cut(s)	331, 480, 723, 1599
BstMBI	GATC	9 cut(s)	316, 432, 495, 529, 910, 1273, 1294, 1332, 1879
BstMCI	CGRYCG	1 cut(s)	364
BstMWI	GCNNNNNNNGC	8 cut(s)	140, 173, 467, 761, 1007, 1058, 1091, 1621
BstNI	CCWGG	5 cut(s)	79, 118, 1245, 1554, 1797
BstNSI	RCATGY	2 cut(s)	878, 973
BstSCI	CCNGG	8 cut(s)	77, 116, 143, 171, 437, 1243, 1552, 1795
BstSLI	GKGCMC	1 cut(s)	874
BstV1I	GCAGC	2 cut(s)	1185, 1653
BstV2I	GAAGAC	3 cut(s)	292, 790, 1096
BstX2I	RGATCY	1 cut(s)	529
BstYI	RGATCY	1 cut(s)	529
Bsu15I	ATCGAT	1 cut(s)	342
BsuRI	GGCC	5 cut(s)	53, 143, 883, 1080, 1766
BsuTUI	ATCGAT	1 cut(s)	342
BtgI	CCRYGG	1 cut(s)	1053
BtsCI	GGATG	7 cut(s)	30, 379, 523, 667, 823, 1518, 1615
BtsIMutI	CAGTG	1 cut(s)	1240
BveI	ACCTGC	1 cut(s)	1052
Cac8I	GCNNGC	9 cut(s)	51, 275, 279, 291, 463, 683, 1171, 1375, 1764
CaiI	CAGNNNCTG	1 cut(s)	919
CciI	TCATGA	3 cut(s)	1524, 1708, 1782
CfoI	GCGC	2 cut(s)	49, 233
Cfr10I	RCCGGY	1 cut(s)	36
Cfr13I	GGNCC	5 cut(s)	39, 142, 259, 881, 1738
ClaI	ATCGAT	1 cut(s)	342
CseI	GACGC	2 cut(s)	433, 467
CsiI	ACCWGGT	2 cut(s)	77, 1795
Csp6I	GTAC	8 cut(s)	349, 973, 1382, 1453, 1520, 1642, 1748, 1793
CspAI	ACCGGT	1 cut(s)	36
CviQI	GTAC	8 cut(s)	349, 973, 1382, 1453, 1520, 1642, 1748, 1793
DdeI	CTNAG	5 cut(s)	203, 783, 1574, 1620, 1742
DpnI	GATC	9 cut(s)	318, 434, 497, 531, 912, 1275, 1296, 1334, 1881
DpnII	GATC	9 cut(s)	316, 432, 495, 529, 910, 1273, 1294, 1332, 1879
DraI	TTTAAA	1 cut(s)	484
DraIII	CACNNNGTG	2 cut(s)	441, 770
EaeI	YGGCCR	2 cut(s)	1078, 1764
Eam1104I	CTCTTC	3 cut(s)	611, 930, 1719
EarI	CTCTTC	3 cut(s)	611, 930, 1719
EciI	GGCGGA	1 cut(s)	37
Ecl136II	GAGCTC	1 cut(s)	685
Eco130I	CCWWGG	1 cut(s)	1053
Eco24I	GRGCYC	1 cut(s)	687
Eco47I	GGWCC	3 cut(s)	39, 259, 1738
Eco53kI	GAGCTC	1 cut(s)	685
Eco57I	CTGAAG	1 cut(s)	1906
Eco88I	CYCGRG	2 cut(s)	567, 686
EcoICRI	GAGCTC	1 cut(s)	685
EcoRI	GAATTC	2 cut(s)	1229, 1786
EcoRII	CCWGG	5 cut(s)	77, 116, 1243, 1552, 1795
EcoT14I	CCWWGG	1 cut(s)	1053
EcoT22I	ATGCAT	4 cut(s)	30, 379, 667, 1077
EcoT38I	GRGCYC	1 cut(s)	687
ErhI	CCWWGG	1 cut(s)	1053
FalI	AAGNNNNNCTT	2 cut(s)	608, 640
FaqI	GGGAC	2 cut(s)	25, 597
FauNDI	CATATG	1 cut(s)	667
FbaI	TGATCA	2 cut(s)	432, 910
FblI	GTMKAC	2 cut(s)	96, 953
Fnu4HI	GCNGC	4 cut(s)	151, 679, 1174, 1667
FokI	GGATG	7 cut(s)	37, 386, 530, 674, 830, 1525, 1602
FriOI	GRGCYC	1 cut(s)	687
Fsp4HI	GCNGC	4 cut(s)	151, 679, 1174, 1667
FspBI	CTAG	1 cut(s)	890
GlaI	GCGC	2 cut(s)	48, 232
GluI	GCNGC	4 cut(s)	151, 679, 1174, 1667
GsaI	CCCAGC	2 cut(s)	882, 1491
GsuI	CTGGAG	1 cut(s)	337
HaeIII	GGCC	5 cut(s)	53, 143, 883, 1080, 1766
HapII	CCGG	5 cut(s)	37, 145, 172, 270, 438
HgaI	GACGC	2 cut(s)	433, 467
HhaI	GCGC	2 cut(s)	49, 233
Hin1I	GRCGYC	3 cut(s)	425, 459, 825
Hin6I	GCGC	2 cut(s)	47, 231
HinP1I	GCGC	2 cut(s)	47, 231
HincII	GTYRAC	2 cut(s)	97, 994
HindII	GTYRAC	2 cut(s)	97, 994
HindIII	AAGCTT	1 cut(s)	194
HinfI	GANTC	9 cut(s)	4, 155, 311, 1531, 1557, 1779, 1807, 1822, 1901
HpaII	CCGG	5 cut(s)	37, 145, 172, 270, 438
HphI	GGTGA	5 cut(s)	275, 427, 625, 833, 1304
Hpy166II	GTNNAC	7 cut(s)	97, 829, 852, 872, 954, 994, 1384
Hpy188I	TCNGA	7 cut(s)	206, 316, 784, 921, 1812, 1829, 1898
Hpy8I	GTNNAC	7 cut(s)	97, 829, 852, 872, 954, 994, 1384
Hpy99I	CGWCG	4 cut(s)	98, 458, 461, 1807
HpyAV	CCTTC	6 cut(s)	232, 272, 1000, 1018, 1723, 1864
HpyCH4III	ACNGT	4 cut(s)	387, 598, 1425, 1852
HpyCH4IV	ACGT	8 cut(s)	93, 411, 425, 825, 845, 982, 1285, 1713
HpyF10VI	GCNNNNNNNGC	8 cut(s)	140, 173, 467, 761, 1007, 1058, 1091, 1621
HpyF3I	CTNAG	5 cut(s)	203, 783, 1574, 1620, 1742
HpySE526I	ACGT	8 cut(s)	93, 411, 425, 825, 845, 982, 1285, 1713
Hsp92I	GRCGYC	3 cut(s)	425, 459, 825
HspAI	GCGC	2 cut(s)	47, 231
Ksp22I	TGATCA	2 cut(s)	432, 910
Kzo9I	GATC	9 cut(s)	316, 432, 495, 529, 910, 1273, 1294, 1332, 1879
LmnI	GCTCC	2 cut(s)	147, 221
Lsp1109I	GCAGC	2 cut(s)	1185, 1653
LweI	GCATC	6 cut(s)	15, 347, 364, 652, 1474, 1624
MabI	ACCWGGT	2 cut(s)	77, 1795
MaeI	CTAG	1 cut(s)	890
MaeII	ACGT	8 cut(s)	93, 411, 425, 825, 845, 982, 1285, 1713
MaeIII	GTNAC	5 cut(s)	415, 821, 841, 1129, 1155
MalI	GATC	9 cut(s)	318, 434, 497, 531, 912, 1275, 1296, 1334, 1881
MbiI	CCGCTC	2 cut(s)	150, 216
MboI	GATC	9 cut(s)	316, 432, 495, 529, 910, 1273, 1294, 1332, 1879
MfeI	CAATTG	1 cut(s)	1840
MflI	RGATCY	1 cut(s)	529
MhlI	GDGCHC	3 cut(s)	687, 874, 1257
MlyI	GAGTC	2 cut(s)	149, 320
MmeI	TCCRAC	2 cut(s)	1077, 1582
Mph1103I	ATGCAT	4 cut(s)	30, 379, 667, 1077
MroXI	GAANNNNTTC	2 cut(s)	196, 386
MseI	TTAA	6 cut(s)	57, 483, 1212, 1299, 1316, 1857
MslI	CAYNNNNRTG	4 cut(s)	834, 1080, 1389, 1446
MspI	CCGG	5 cut(s)	37, 145, 172, 270, 438
MspR9I	CCNGG	8 cut(s)	79, 118, 145, 173, 439, 1245, 1554, 1797
MunI	CAATTG	1 cut(s)	1840
Mva1269I	GAATGC	1 cut(s)	1077
MvaI	CCWGG	5 cut(s)	79, 118, 1245, 1554, 1797
MwoI	GCNNNNNNNGC	8 cut(s)	140, 173, 467, 761, 1007, 1058, 1091, 1621
NciI	CCSGG	3 cut(s)	145, 173, 439
NcoI	CCATGG	1 cut(s)	1053
NdeI	CATATG	1 cut(s)	667
NdeII	GATC	9 cut(s)	316, 432, 495, 529, 910, 1273, 1294, 1332, 1879
NlaIV	GGNNCC	1 cut(s)	41
NmeAIII	GCCGAG	1 cut(s)	227
NmuCI	GTSAC	4 cut(s)	821, 841, 1129, 1155
NsiI	ATGCAT	4 cut(s)	30, 379, 667, 1077
NspI	RCATGY	2 cut(s)	878, 973
PaeR7I	CTCGAG	2 cut(s)	567, 686
PagI	TCATGA	3 cut(s)	1524, 1708, 1782
PciI	ACATGT	1 cut(s)	969
PcsI	WCGNNNNNNNCGW	1 cut(s)	102
PctI	GAATGC	1 cut(s)	1077
PdmI	GAANNNNTTC	2 cut(s)	196, 386
PfeI	GAWTC	6 cut(s)	1531, 1557, 1779, 1807, 1822, 1901
PflFI	GACNNNGTC	2 cut(s)	107, 847
PfoI	TCCNGGA	2 cut(s)	116, 1552
PinAI	ACCGGT	1 cut(s)	36
PkrI	GCNGC	4 cut(s)	152, 680, 1175, 1668
PleI	GAGTC	2 cut(s)	149, 319
PpsI	GAGTC	2 cut(s)	149, 319
PscI	ACATGT	1 cut(s)	969
Psp124BI	GAGCTC	1 cut(s)	687
Psp6I	CCWGG	5 cut(s)	77, 116, 1243, 1552, 1795
PspFI	CCCAGC	2 cut(s)	878, 1487
PspGI	CCWGG	5 cut(s)	77, 116, 1243, 1552, 1795
PspN4I	GGNNCC	1 cut(s)	41
PspPI	GGNCC	5 cut(s)	39, 142, 259, 881, 1738
PspXI	VCTCGAGB	1 cut(s)	686
PstNI	CAGNNNCTG	1 cut(s)	919
PsuI	RGATCY	1 cut(s)	529
PsyI	GACNNNGTC	2 cut(s)	107, 847
RsaI	GTAC	8 cut(s)	350, 974, 1383, 1454, 1521, 1643, 1749, 1794
RsaNI	GTAC	8 cut(s)	349, 973, 1382, 1453, 1520, 1642, 1748, 1793
RseI	CAYNNNNRTG	4 cut(s)	834, 1080, 1389, 1446
SacI	GAGCTC	1 cut(s)	687
SalI	GTCGAC	1 cut(s)	95
SaqAI	TTAA	6 cut(s)	57, 483, 1212, 1299, 1316, 1857
SatI	GCNGC	4 cut(s)	151, 679, 1174, 1667
Sau3AI	GATC	9 cut(s)	316, 432, 495, 529, 910, 1273, 1294, 1332, 1879
Sau96I	GGNCC	5 cut(s)	39, 142, 259, 881, 1738
ScaI	AGTACT	2 cut(s)	1521, 1643
SchI	GAGTC	2 cut(s)	149, 320
ScrFI	CCNGG	8 cut(s)	79, 118, 145, 173, 439, 1245, 1554, 1797
SduI	GDGCHC	3 cut(s)	687, 874, 1257
SexAI	ACCWGGT	2 cut(s)	77, 1795
SfaNI	GCATC	6 cut(s)	15, 347, 364, 652, 1474, 1624
Sfr274I	CTCGAG	2 cut(s)	567, 686
SinI	GGWCC	3 cut(s)	39, 259, 1738
SlaI	CTCGAG	2 cut(s)	567, 686
SmiMI	CAYNNNNRTG	4 cut(s)	834, 1080, 1389, 1446
SmlI	CTYRAG	2 cut(s)	567, 686
SmoI	CTYRAG	2 cut(s)	567, 686
SsiI	CCGC	6 cut(s)	22, 150, 214, 360, 678, 1909
SspMI	CTAG	1 cut(s)	890
SstI	GAGCTC	1 cut(s)	687
StyD4I	CCNGG	8 cut(s)	77, 116, 143, 171, 437, 1243, 1552, 1795
StyI	CCWWGG	1 cut(s)	1053
TaaI	ACNGT	4 cut(s)	387, 598, 1425, 1852
TaiI	ACGT	8 cut(s)	96, 414, 428, 828, 848, 985, 1288, 1716
TaqI	TCGA	9 cut(s)	96, 162, 342, 364, 390, 498, 568, 687, 966
TatI	WGTACW	5 cut(s)	348, 972, 1519, 1641, 1747
TauI	GCSGC	2 cut(s)	153, 681
TfiI	GAWTC	6 cut(s)	1531, 1557, 1779, 1807, 1822, 1901
Tru1I	TTAA	6 cut(s)	57, 483, 1212, 1299, 1316, 1857
Tru9I	TTAA	6 cut(s)	57, 483, 1212, 1299, 1316, 1857
TscAI	CASTG	1 cut(s)	1240
TseFI	GTSAC	4 cut(s)	821, 841, 1129, 1155
TseI	GCWGC	2 cut(s)	1173, 1666
Tsp45I	GTSAC	4 cut(s)	821, 841, 1129, 1155
TspDTI	ATGAA	7 cut(s)	590, 1037, 1350, 1541, 1697, 1799, 1904
TspGWI	ACGGA	3 cut(s)	115, 386, 819
TspRI	CASTG	1 cut(s)	1240
Tth111I	GACNNNGTC	2 cut(s)	107, 847
VneI	GTGCAC	1 cut(s)	870
VpaK11BI	GGWCC	3 cut(s)	39, 259, 1738
XapI	RAATTY	2 cut(s)	1229, 1786
XceI	RCATGY	2 cut(s)	878, 973
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1093
XhoI	CTCGAG	2 cut(s)	567, 686
XmiI	GTMKAC	2 cut(s)	96, 953
XmnI	GAANNNNTTC	2 cut(s)	196, 386
XspI	CTAG	1 cut(s)	890
ZraI	GACGTC	2 cut(s)	426, 826
ZrmI	AGTACT	2 cut(s)	1521, 1643
Zsp2I	ATGCAT	4 cut(s)	30, 379, 667, 1077

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.