pycom17g07140

twin BRCT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004814
Physical Location & Seq
Forward (+)
5145924 .. 5147833
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 888 bp
ATGGCTGGAACTTCACGTAAAGGAAGGAAGCTTATAAAAAAGAATATTGATAGACATATGGTAGAATCGGAACTCTCAGAGTATGATCAAGAGTGCCACCCAACCAGATTTCAGAGTACATGTAATGATGTAATCACAGTCCTTTCTGATGATTCAGATGAAGAAAGAAATGATAATATACTGGAAACTGAAAGAGATACTGATAATGGCTTGTGCATTGGAAGAAGAATCAGAAATGGTTTGAAAGGTGTTGAGGAGGCTGATCGGTATCACCCATCTTCTTCCAAGCATCCAAATATTTCAGTTGAGGATGAACCAACTGCTCTGGAGGAAACTCCCCGAGATGAATGGTTTGAAGTTGAGAACTCAAAAGAGGAATCTGAAAGCAGATTTGAGACTGAAGCTGCCACCAAAATTCCTTCGATGGAGTTATCATGTGTAATATGCTGGACAGAATATAGTTCAATTCGAGGAATTTTGCCATGTGGTCATCGATTCTGTTACTCATGCATTGAAAACTGGGCTGGTCAGATGAGTTCAAAGAGAAAGAATTCAACTTGCCCCTTATGCAAGGCTAGTTTTACAAGCATTACCAAGGTTGATGATGCAGACATGATTGATCAGAAAATATACTCCCAAACCATTCCATCTGCCCCGAAAATGGACATACCAGTGCCTACTTACCGAGGACCACCCAATTTTTCTGCTCCGTCCATATCAGCAATTGCTTGTTCTGTATGTCGTCAGCGGGAACCTGTAGATCTTCTGTTCAGTTGTGACGTCTGCCACATTCGGTGCATCCATAACTTCTGCCTGGACCCTCCTTTGTTTCCCTGGACATGCATTCACTGCAAGGACCTCCGACGGCTCTACCTTCACAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00590 ko04918 map00480 map00590 map04918
Pfam Domains
Protein Families
PTCB-BRCT zf-C3HC4 zf-RING_2

Protein Analysis

296

Amino Acids

33.67

Weight (kDa)

5.37

Isoelectric Point (pI)

58.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 144 - 196 6.2e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 145 - 191 1.1e-09 Ring finger domain
zf-C3HC4 PF00097 146 - 190 5.8e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 146 - 188 1.1e-07 RING-type zinc-finger
zf-RING_5 PF14634 146 - 191 2.6e-06 zinc-RING finger domain
zf-C3HC4_2 PF13923 146 - 190 4.6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 35
AatII GACGTC 1 cut(s) 783
AciI CCGC 1 cut(s) 748
AcsI RAATTY 3 cut(s) 414, 474, 550
AcuI CTGAAG 1 cut(s) 420
AcyI GRCGYC 1 cut(s) 780
AfaI GTAC 1 cut(s) 118
AfiI CCNNNNNNNGG 1 cut(s) 661
AflIII ACRYGT 1 cut(s) 119
AgsI TTSAA 6 cut(s) 244, 356, 465, 515, 540, 555
AjnI CCWGG 2 cut(s) 813, 833
AluBI AGCT 3 cut(s) 31, 404, 882
AluI AGCT 3 cut(s) 31, 404, 882
Alw26I GTCTC 1 cut(s) 389
Ama87I CYCGRG 1 cut(s) 339
ApeKI GCWGC 1 cut(s) 404
ApoI RAATTY 3 cut(s) 414, 474, 550
Asp700I GAANNNNTTC 1 cut(s) 550
AspS9I GGNCC 3 cut(s) 689, 817, 856
AsuHPI GGTGA 1 cut(s) 263
AvaI CYCGRG 1 cut(s) 339
AvaII GGWCC 3 cut(s) 689, 817, 856
BbvI GCAGC 1 cut(s) 391
BccI CCATC 3 cut(s) 283, 418, 655
BceAI ACGGC 1 cut(s) 881
BciT130I CCWGG 2 cut(s) 815, 835
BclI TGATCA 2 cut(s) 85, 619
BcoDI GTCTC 1 cut(s) 389
BfaI CTAG 1 cut(s) 576
BfmI CTRYAG 1 cut(s) 756
BglII AGATCT 1 cut(s) 760
BisI GCNGC 1 cut(s) 405
BlsI GCNGC 1 cut(s) 406
Bme1390I CCNGG 2 cut(s) 815, 835
Bme18I GGWCC 3 cut(s) 689, 817, 856
BmeT110I CYCGRG 1 cut(s) 339
BmgT120I GGNCC 3 cut(s) 689, 817, 856
BmiI GGNNCC 2 cut(s) 753, 819
BmrFI CCNGG 2 cut(s) 815, 835
BmrI ACTGGG 1 cut(s) 529
BmsI GCATC 3 cut(s) 298, 595, 807
BmuI ACTGGG 1 cut(s) 529
BpmI CTGGAG 1 cut(s) 347
Bsa29I ATCGAT 1 cut(s) 493
BsaAI YACGTR 1 cut(s) 17
BsaBI GATNNNNATC 1 cut(s) 267
BsaHI GRCGYC 1 cut(s) 780
BsaJI CCNNGG 3 cut(s) 594, 685, 833
Bsc4I CCNNNNNNNGG 1 cut(s) 661
Bse1I ACTGG 3 cut(s) 186, 524, 671
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 2 cut(s) 815, 835
BseCI ATCGAT 1 cut(s) 493
BseDI CCNNGG 3 cut(s) 594, 685, 833
BseGI GGATG 3 cut(s) 289, 316, 798
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 1 cut(s) 661
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 3 cut(s) 186, 524, 671
BseRI GAGGAG 1 cut(s) 269
BseXI GCAGC 1 cut(s) 391
BshVI ATCGAT 1 cut(s) 493
BsiHKCI CYCGRG 1 cut(s) 339
BslI CCNNNNNNNGG 1 cut(s) 661
BsmAI GTCTC 1 cut(s) 389
BsmI GAATGC 1 cut(s) 843
BsoBI CYCGRG 1 cut(s) 339
Bsp143I GATC 4 cut(s) 85, 262, 619, 760
BspACI CCGC 1 cut(s) 748
BspCNI CTCAG 1 cut(s) 89
BspDI ATCGAT 1 cut(s) 493
BspLI GGNNCC 2 cut(s) 753, 819
BsrI ACTGG 3 cut(s) 186, 524, 671
BssECI CCNNGG 3 cut(s) 594, 685, 833
BssMI GATC 4 cut(s) 85, 262, 619, 760
BssNI GRCGYC 1 cut(s) 780
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 2 cut(s) 815, 835
Bst4CI ACNGT 1 cut(s) 139
BstACI GRCGYC 1 cut(s) 780
BstAPI GCANNNNNTGC 1 cut(s) 849
BstBAI YACGTR 1 cut(s) 17
BstDEI CTNAG 1 cut(s) 76
BstF5I GGATG 3 cut(s) 289, 316, 798
BstKTI GATC 4 cut(s) 88, 265, 622, 763
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 4 cut(s) 85, 262, 619, 760
BstMWI GCNNNNNNNGC 2 cut(s) 567, 849
BstNI CCWGG 2 cut(s) 815, 835
BstNSI RCATGY 2 cut(s) 123, 843
BstSCI CCNGG 2 cut(s) 813, 833
BstSFI CTRYAG 1 cut(s) 756
BstV1I GCAGC 1 cut(s) 391
BstX2I RGATCY 1 cut(s) 760
BstYI RGATCY 1 cut(s) 760
Bsu15I ATCGAT 1 cut(s) 493
BsuTUI ATCGAT 1 cut(s) 493
BtsCI GGATG 3 cut(s) 289, 316, 798
BtsI GCAGTG 1 cut(s) 847
BtsIMutI CAGTG 2 cut(s) 678, 847
Cfr13I GGNCC 3 cut(s) 689, 817, 856
ClaI ATCGAT 1 cut(s) 493
Csp6I GTAC 1 cut(s) 117
CviAII CATG 6 cut(s) 120, 435, 483, 507, 613, 840
CviJI RGCY 9 cut(s) 5, 31, 210, 260, 404, 524, 575, 868, 882
CviKI_1 RGCY 9 cut(s) 5, 31, 210, 260, 404, 524, 575, 868, 882
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 1 cut(s) 76
DpnI GATC 4 cut(s) 87, 264, 621, 762
DpnII GATC 4 cut(s) 85, 262, 619, 760
Eco130I CCWWGG 1 cut(s) 594
Eco47I GGWCC 3 cut(s) 689, 817, 856
Eco57I CTGAAG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 339
EcoO109I RGGNCCY 1 cut(s) 856
EcoRI GAATTC 1 cut(s) 550
EcoRII CCWGG 2 cut(s) 813, 833
EcoT14I CCWWGG 1 cut(s) 594
EcoT22I ATGCAT 2 cut(s) 512, 845
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 6 cut(s) 123, 438, 486, 510, 616, 843
FatI CATG 6 cut(s) 119, 434, 482, 506, 612, 839
FauI CCCGC 1 cut(s) 741
FauNDI CATATG 1 cut(s) 57
FbaI TGATCA 2 cut(s) 85, 619
Fnu4HI GCNGC 1 cut(s) 405
FokI GGATG 3 cut(s) 276, 323, 785
Fsp4HI GCNGC 1 cut(s) 405
FspBI CTAG 1 cut(s) 576
GluI GCNGC 1 cut(s) 405
GsuI CTGGAG 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 780
Hin1II CATG 6 cut(s) 123, 438, 486, 510, 616, 843
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 5 cut(s) 65, 152, 228, 377, 495
HphI GGTGA 1 cut(s) 263
Hpy188III TCNNGA 2 cut(s) 89, 326
Hpy99I CGWCG 1 cut(s) 867
HpyAV CCTTC 3 cut(s) 18, 429, 884
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4IV ACGT 2 cut(s) 16, 780
HpyCH4V TGCA 7 cut(s) 216, 510, 570, 608, 798, 843, 852
HpyF10VI GCNNNNNNNGC 2 cut(s) 567, 849
HpyF3I CTNAG 1 cut(s) 76
HpySE526I ACGT 2 cut(s) 16, 780
Hsp92I GRCGYC 1 cut(s) 780
Hsp92II CATG 6 cut(s) 123, 438, 486, 510, 616, 843
Ksp22I TGATCA 2 cut(s) 85, 619
Kzo9I GATC 4 cut(s) 85, 262, 619, 760
LmnI GCTCC 1 cut(s) 712
Lsp1109I GCAGC 1 cut(s) 391
LweI GCATC 3 cut(s) 298, 595, 807
MaeI CTAG 1 cut(s) 576
MaeII ACGT 2 cut(s) 16, 780
MaeIII GTNAC 2 cut(s) 500, 776
MalI GATC 4 cut(s) 87, 264, 621, 762
MboI GATC 4 cut(s) 85, 262, 619, 760
MboII GAAGA 6 cut(s) 173, 234, 237, 270, 273, 755
MfeI CAATTG 1 cut(s) 723
MflI RGATCY 1 cut(s) 760
MluCI AATT 6 cut(s) 414, 465, 474, 550, 697, 723
MmeI TCCRAC 1 cut(s) 886
MnlI CCTC 9 cut(s) 247, 250, 301, 322, 367, 464, 680, 831, 869
Mph1103I ATGCAT 2 cut(s) 512, 845
MroXI GAANNNNTTC 1 cut(s) 550
MslI CAYNNNNRTG 1 cut(s) 671
MspA1I CMGCKG 1 cut(s) 748
MspR9I CCNGG 2 cut(s) 815, 835
MunI CAATTG 1 cut(s) 723
Mva1269I GAATGC 1 cut(s) 843
MvaI CCWGG 2 cut(s) 815, 835
MwoI GCNNNNNNNGC 2 cut(s) 567, 849
NdeI CATATG 1 cut(s) 57
NdeII GATC 4 cut(s) 85, 262, 619, 760
NlaIII CATG 6 cut(s) 123, 438, 486, 510, 616, 843
NlaIV GGNNCC 2 cut(s) 753, 819
NmuCI GTSAC 1 cut(s) 776
NsiI ATGCAT 2 cut(s) 512, 845
NspI RCATGY 2 cut(s) 123, 843
PciI ACATGT 1 cut(s) 119
PctI GAATGC 1 cut(s) 843
PdmI GAANNNNTTC 1 cut(s) 550
PfeI GAWTC 5 cut(s) 65, 152, 228, 377, 495
PkrI GCNGC 1 cut(s) 406
Ppu21I YACGTR 1 cut(s) 17
PpuMI RGGWCCY 1 cut(s) 856
PscI ACATGT 1 cut(s) 119
PsiI TTATAA 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 856
Psp6I CCWGG 2 cut(s) 813, 833
PspGI CCWGG 2 cut(s) 813, 833
PspN4I GGNNCC 2 cut(s) 753, 819
PspPI GGNCC 3 cut(s) 689, 817, 856
PspPPI RGGWCCY 1 cut(s) 856
PsuI RGATCY 1 cut(s) 760
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
RseI CAYNNNNRTG 1 cut(s) 671
SatI GCNGC 1 cut(s) 405
Sau3AI GATC 4 cut(s) 85, 262, 619, 760
Sau96I GGNCC 3 cut(s) 689, 817, 856
ScrFI CCNGG 2 cut(s) 815, 835
SfaNI GCATC 3 cut(s) 298, 595, 807
SfcI CTRYAG 1 cut(s) 756
SinI GGWCC 3 cut(s) 689, 817, 856
SmiMI CAYNNNNRTG 1 cut(s) 671
Sse9I AATT 6 cut(s) 414, 465, 474, 550, 697, 723
SsiI CCGC 1 cut(s) 748
SspI AATATT 2 cut(s) 46, 298
SspMI CTAG 1 cut(s) 576
StyD4I CCNGG 2 cut(s) 813, 833
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 1 cut(s) 139
TaiI ACGT 2 cut(s) 19, 783
TaqI TCGA 3 cut(s) 422, 469, 493
TasI AATT 6 cut(s) 414, 465, 474, 550, 697, 723
TatI WGTACW 1 cut(s) 116
TfiI GAWTC 5 cut(s) 65, 152, 228, 377, 495
TscAI CASTG 2 cut(s) 678, 854
TseFI GTSAC 1 cut(s) 776
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 776
TspDTI ATGAA 3 cut(s) 174, 327, 360
TspGWI ACGGA 1 cut(s) 699
TspRI CASTG 2 cut(s) 678, 854
VpaK11BI GGWCC 3 cut(s) 689, 817, 856
XapI RAATTY 3 cut(s) 414, 474, 550
XceI RCATGY 2 cut(s) 123, 843
XmnI GAANNNNTTC 1 cut(s) 550
XspI CTAG 1 cut(s) 576
ZraI GACGTC 1 cut(s) 781
Zsp2I ATGCAT 2 cut(s) 512, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.