pycom17g12320

Leucine-rich repeat receptor-like kinase protein FLORAL ORGAN NUMBER1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0013307
Physical Location & Seq
Forward (+)
10013272 .. 10014525
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g12320.1

Sequence Viewer

Length: 1254 bp
ATGGCCAAACCACACTCTCACTCCCCACTCCCACTCTCACTCCTCCTCCTCCTCCTCTCACTCCTCCTCACTCCCTCCCTCCAACGAACCCAGAAACTCTCCCACGTCGATCTCCGCGCCCTCATCGCAATCAAAAACTCCCTCACCGACGTCCCTTCCCGCCGTGGCTCCCCAGCCGCATTCTTCTCCACCTGGGACTTCTCCGCCCCCGACCCCTGCTCCACCTTCTCCGGCCTCACCTGCGCTTCCGGCCGAGTCACTACCCTCACCCTCGGCACTGGCCTCTCCGACTCCCCCGGTCTTGCCGGGTCGCTCCCGCCCTCCATCGCCGACCTCACCGAGTTAACTCAGCTAATCCTCTTCCCCGGAATAGTCACCGGTCCGATCCCGTTTCAGCTCGGTTGGCTCGCCAGCCTCCGTGTCATCTCCCTAACCAACAACCGTCTCACTGGCCGGATTCCTCAGTCCCTATCTCTCCTCCCCAATCTCCACACCCTTGACCTCAGCTACAACAGCCTCGCGGGCCCTATCCCGCCGGGTCTGACCCAGTTACCCAACCTCAAGGTCCTGATCCTCGCCTCCAATTCCCTCTCCGGCGAGGTCCCCTACAACGTGTCGTCCCAGCTACTTCACTTGGACTTCAACCGCAACGGCCTCACCGGCCCGTTACCACCCTCTCTCCCGACGACGCTCCGTTACGTGTCGCTCTCGGAAAACGACATGTCGGGCCCCATCAGCTCCGTCTTCGACTCGCTCGCCGATTTAGTCCACCTCAATCTCGGGTCCAACAGTTTCAGTGGGCCCCTCCCGCAAACTCTGTTCCGCACGACCCTCCAGTCCCTCCTGTTGCAACGGAACAATTTCTCCGGGTGGGTCCCGCAGGCAGGGTCCCAGCCCCCATCGTACGGTGAGGGTTCGATCGTGGATTTGAGCCACAATGCCATAACCGGTGAGCTGTCGCCGATCTTGGCGGGTGTGGAGAGCTTGTATTTGAATAACAACCGTCTGATGGGGTCGGTGCCGAGGGAGTACGTTAAGAGCGTGTACGTGGGGACCACCAGAACGTTGTACCTGCAGCACAATTACATTTCCCGGTTCCCGGTCGAGCCCGGGTCGATGTTCCCCGATACAGTGTCGCTGTGCTTGAGCTACAACTGCATGGTGCCGCCCGTGGGGATGGCGGCGTGCCCGGCCAGCGCGGGAACGGAGCTGTCGAGGCCGGCGTCGCAGTGCTCGGTGTTTAACTACGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8

Protein Analysis

418

Amino Acids

44.47

Weight (kDa)

7.07

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 138 - 173 9.6e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013307)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17640
fragaria_vesca FvH4_6g39240
malus_domestica MD09G1144100.v1.1 MD17G1130800.v1.1
prunus_persica Prupe.3G183800_v2.0.a1
pyrus_communis pycom09g06350 pycom17g12320
rosa_chinensis RchiOBHm_Chr2g0153081
rosa_laevigata RLG00000020675
rosa_multiflora Rmu_sc0000218.1_g000028 Rmu_sc0036105.1_g000001
rosa_roxburghii Rroxscaffold_2G00095210
rosa_rugosa Rorug02G0437200
rosa_samantha Rh2AG499400 Rh2BG510700 Rh2CG485800 Rh2DG522400
rosa_wichuraiana Rw2G040990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 248
AasI GACNNNNNNGTC 1 cut(s) 835
AatII GACGTC 1 cut(s) 153
Acc36I ACCTGC 2 cut(s) 248, 1080
AccB1I GGYRCC 2 cut(s) 1018, 1162
AccII CGCG 3 cut(s) 117, 521, 1199
AclI AACGTT 1 cut(s) 1064
AclWI GGATC 2 cut(s) 379, 565
AcoI YGGCCR 4 cut(s) 3, 250, 451, 1191
AcyI GRCGYC 2 cut(s) 150, 1223
AfaI GTAC 4 cut(s) 905, 1031, 1046, 1070
AfiI CCNNNNNNNGG 5 cut(s) 884, 905, 1009, 1099, 1172
AflIII ACRYGT 3 cut(s) 612, 699, 720
AgeI ACCGGT 2 cut(s) 377, 947
AgsI TTSAA 2 cut(s) 643, 994
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 1 cut(s) 191
AloI GAACNNNNNNTCC 2 cut(s) 848, 880
AluBI AGCT 9 cut(s) 352, 397, 507, 625, 738, 955, 984, 1149, 1210
AluI AGCT 9 cut(s) 352, 397, 507, 625, 738, 955, 984, 1149, 1210
Alw21I GWGCWC 1 cut(s) 1235
Alw26I GTCTC 1 cut(s) 449
AlwI GGATC 2 cut(s) 379, 565
Ama87I CYCGRG 2 cut(s) 779, 1109
ApaI GGGCCC 3 cut(s) 527, 731, 804
ApeKI GCWGC 1 cut(s) 1075
AsiGI ACCGGT 2 cut(s) 377, 947
Asp700I GAANNNNTTC 1 cut(s) 860
AspLEI GCGC 3 cut(s) 119, 245, 1199
AsuHPI GGTGA 8 cut(s) 136, 229, 259, 328, 367, 649, 920, 962
AvaI CYCGRG 2 cut(s) 779, 1109
AvaII GGWCC 7 cut(s) 380, 565, 601, 783, 874, 888, 1053
BaeGI GKGCMC 4 cut(s) 527, 731, 804, 1190
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 1018, 1162
BanII GRGCYC 4 cut(s) 527, 731, 804, 1110
BbsI GAAGAC 1 cut(s) 736
Bbv12I GWGCWC 1 cut(s) 1235
BbvCI CCTCAGC 1 cut(s) 503
BbvI GCAGC 1 cut(s) 1087
BccI CCATC 5 cut(s) 332, 740, 907, 1003, 1171
BceAI ACGGC 2 cut(s) 147, 667
BciT130I CCWGG 1 cut(s) 193
BcoDI GTCTC 1 cut(s) 449
BfmI CTRYAG 1 cut(s) 1073
BfuAI ACCTGC 2 cut(s) 248, 1080
BglI GCCNNNNNGGC 2 cut(s) 522, 660
BisI GCNGC 4 cut(s) 177, 1076, 1166, 1182
BlsI GCNGC 4 cut(s) 178, 1077, 1167, 1183
Bme18I GGWCC 7 cut(s) 380, 565, 601, 783, 874, 888, 1053
BmeT110I CYCGRG 2 cut(s) 779, 1109
BmgBI CACGTC 1 cut(s) 106
BmrI ACTGGG 1 cut(s) 541
BmuI ACTGGG 1 cut(s) 541
BpiI GAAGAC 1 cut(s) 736
BpmI CTGGAG 1 cut(s) 818
Bpu10I CCTNAGC 1 cut(s) 503
BpuEI CTTGAG 2 cut(s) 545, 1165
BsaAI YACGTR 2 cut(s) 700, 1048
BsaHI GRCGYC 2 cut(s) 150, 1223
BsaJI CCNNGG 8 cut(s) 163, 192, 271, 295, 364, 1022, 1109, 1170
BsaWI WCCGGW 2 cut(s) 377, 947
Bsc4I CCNNNNNNNGG 5 cut(s) 884, 905, 1009, 1099, 1172
Bse118I RCCGGY 4 cut(s) 377, 659, 947, 1219
Bse1I ACTGG 4 cut(s) 283, 454, 547, 835
BseBI CCWGG 1 cut(s) 193
BseDI CCNNGG 8 cut(s) 163, 192, 271, 295, 364, 1022, 1109, 1170
BseGI GGATG 1 cut(s) 1182
BseLI CCNNNNNNNGG 5 cut(s) 884, 905, 1009, 1099, 1172
BseMII CTCAG 3 cut(s) 362, 476, 517
BseNI ACTGG 4 cut(s) 283, 454, 547, 835
BseRI GAGGAG 8 cut(s) 32, 35, 38, 41, 44, 53, 56, 467
BseSI GKGCMC 4 cut(s) 527, 731, 804, 1190
BseX3I CGGCCG 1 cut(s) 250
BseXI GCAGC 1 cut(s) 1087
BseYI CCCAGC 3 cut(s) 172, 621, 891
Bsh1236I CGCG 3 cut(s) 117, 521, 1199
Bsh1285I CGRYCG 3 cut(s) 253, 921, 1104
BshNI GGYRCC 2 cut(s) 1018, 1162
BshTI ACCGGT 2 cut(s) 377, 947
BsiEI CGRYCG 3 cut(s) 253, 921, 1104
BsiHKAI GWGCWC 1 cut(s) 1235
BsiHKCI CYCGRG 2 cut(s) 779, 1109
BsiWI CGTACG 1 cut(s) 903
BslFI GGGAC 9 cut(s) 137, 209, 451, 587, 604, 823, 860, 874, 1066
BslI CCNNNNNNNGG 5 cut(s) 884, 905, 1009, 1099, 1172
BsmAI GTCTC 1 cut(s) 449
BsmBI CGTCTC 1 cut(s) 449
BsmFI GGGAC 9 cut(s) 137, 209, 451, 587, 604, 823, 860, 874, 1066
BsmI GAATGC 1 cut(s) 179
BsoBI CYCGRG 2 cut(s) 779, 1109
Bsp120I GGGCCC 3 cut(s) 523, 727, 800
Bsp1286I GDGCHC 6 cut(s) 527, 731, 804, 1110, 1190, 1235
Bsp143I GATC 5 cut(s) 109, 384, 570, 918, 963
BspCNI CTCAG 3 cut(s) 361, 475, 516
BspFNI CGCG 3 cut(s) 117, 521, 1199
BspMAI CTGCAG 1 cut(s) 1077
BspMI ACCTGC 2 cut(s) 248, 1080
BspPI GGATC 2 cut(s) 379, 565
BspT107I GGYRCC 2 cut(s) 1018, 1162
BsrFI RCCGGY 4 cut(s) 377, 659, 947, 1219
BsrI ACTGG 4 cut(s) 283, 454, 547, 835
BssAI RCCGGY 4 cut(s) 377, 659, 947, 1219
BssECI CCNNGG 8 cut(s) 163, 192, 271, 295, 364, 1022, 1109, 1170
BssMI GATC 5 cut(s) 109, 384, 570, 918, 963
BssNI GRCGYC 2 cut(s) 150, 1223
Bst2UI CCWGG 1 cut(s) 193
Bst4CI ACNGT 5 cut(s) 443, 791, 908, 1004, 1132
Bst6I CTCTTC 1 cut(s) 365
BstACI GRCGYC 2 cut(s) 150, 1223
BstBAI YACGTR 2 cut(s) 700, 1048
BstC8I GCNNGC 8 cut(s) 408, 412, 523, 756, 882, 1186, 1195, 1221
BstDEI CTNAG 3 cut(s) 348, 462, 503
BstDSI CCRYGG 2 cut(s) 163, 1170
BstF5I GGATG 1 cut(s) 1182
BstFNI CGCG 3 cut(s) 117, 521, 1199
BstHHI GCGC 3 cut(s) 119, 245, 1199
BstKTI GATC 5 cut(s) 112, 387, 573, 921, 966
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 5 cut(s) 109, 384, 570, 918, 963
BstMCI CGRYCG 3 cut(s) 253, 921, 1104
BstNI CCWGG 1 cut(s) 193
BstNSI RCATGY 1 cut(s) 724
BstSFI CTRYAG 1 cut(s) 1073
BstSLI GKGCMC 4 cut(s) 527, 731, 804, 1190
BstUI CGCG 3 cut(s) 117, 521, 1199
BstV1I GCAGC 1 cut(s) 1087
BstV2I GAAGAC 1 cut(s) 736
BstZI CGGCCG 1 cut(s) 250
BtgI CCRYGG 2 cut(s) 163, 1170
BtgZI GCGATG 2 cut(s) 109, 310
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 1 cut(s) 1182
BtsI GCAGTG 1 cut(s) 1235
BtsIMutI CAGTG 5 cut(s) 276, 447, 802, 1137, 1235
BveI ACCTGC 2 cut(s) 248, 1080
Cac8I GCNNGC 8 cut(s) 408, 412, 523, 756, 882, 1186, 1195, 1221
CfoI GCGC 3 cut(s) 119, 245, 1199
Cfr10I RCCGGY 4 cut(s) 377, 659, 947, 1219
Cfr9I CCCGGG 1 cut(s) 1109
CpoI CGGWCCG 1 cut(s) 380
CseI GACGC 2 cut(s) 697, 1212
Csp6I GTAC 4 cut(s) 904, 1030, 1045, 1069
CspAI ACCGGT 2 cut(s) 377, 947
CspI CGGWCCG 1 cut(s) 380
CviAII CATG 2 cut(s) 721, 1159
CviQI GTAC 4 cut(s) 904, 1030, 1045, 1069
DdeI CTNAG 3 cut(s) 348, 462, 503
DpnI GATC 5 cut(s) 111, 386, 572, 920, 965
DpnII GATC 5 cut(s) 109, 384, 570, 918, 963
DrdI GACNNNNNNGTC 1 cut(s) 835
DseDI GACNNNNNNGTC 1 cut(s) 835
EaeI YGGCCR 4 cut(s) 3, 250, 451, 1191
EagI CGGCCG 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 365
EarI CTCTTC 1 cut(s) 365
EciI GGCGGA 1 cut(s) 193
EclXI CGGCCG 1 cut(s) 250
Eco24I GRGCYC 4 cut(s) 527, 731, 804, 1110
Eco47I GGWCC 7 cut(s) 380, 565, 601, 783, 874, 888, 1053
Eco52I CGGCCG 1 cut(s) 250
Eco88I CYCGRG 2 cut(s) 779, 1109
EcoO109I RGGNCCY 7 cut(s) 524, 565, 601, 728, 801, 874, 888
EcoRII CCWGG 1 cut(s) 191
EcoT38I GRGCYC 4 cut(s) 527, 731, 804, 1110
Esp3I CGTCTC 1 cut(s) 449
FaeI CATG 2 cut(s) 724, 1162
FaiI YATR 3 cut(s) 722, 944, 1160
FaqI GGGAC 9 cut(s) 137, 209, 451, 587, 604, 823, 860, 874, 1066
FatI CATG 2 cut(s) 720, 1158
FauI CCCGC 8 cut(s) 167, 324, 514, 540, 816, 885, 964, 1192
Fnu4HI GCNGC 4 cut(s) 177, 1076, 1166, 1182
FokI GGATG 1 cut(s) 1189
FriOI GRGCYC 4 cut(s) 527, 731, 804, 1110
Fsp4HI GCNGC 4 cut(s) 177, 1076, 1166, 1182
GlaI GCGC 3 cut(s) 118, 244, 1198
GluI GCNGC 4 cut(s) 177, 1076, 1166, 1182
GsaI CCCAGC 3 cut(s) 176, 625, 895
GsuI CTGGAG 1 cut(s) 818
HgaI GACGC 2 cut(s) 697, 1212
HhaI GCGC 3 cut(s) 119, 245, 1199
Hin1I GRCGYC 2 cut(s) 150, 1223
Hin1II CATG 2 cut(s) 724, 1162
Hin6I GCGC 3 cut(s) 117, 243, 1197
HinP1I GCGC 3 cut(s) 117, 243, 1197
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HinfI GANTC 4 cut(s) 255, 290, 457, 749
HpaI GTTAAC 1 cut(s) 345
HphI GGTGA 8 cut(s) 136, 229, 259, 328, 367, 649, 920, 962
Hpy166II GTNNAC 3 cut(s) 345, 769, 1045
Hpy188I TCNGA 5 cut(s) 289, 384, 543, 712, 1008
Hpy188III TCNNGA 2 cut(s) 568, 682
Hpy8I GTNNAC 3 cut(s) 345, 769, 1045
Hpy99I CGWCG 5 cut(s) 110, 152, 688, 691, 1228
HpyAV CCTTC 2 cut(s) 165, 235
HpyCH4III ACNGT 5 cut(s) 443, 791, 908, 1004, 1132
HpyCH4IV ACGT 7 cut(s) 105, 150, 612, 699, 1032, 1047, 1064
HpyCH4V TGCA 3 cut(s) 850, 1075, 1158
HpyF3I CTNAG 3 cut(s) 348, 462, 503
HpySE526I ACGT 7 cut(s) 105, 150, 612, 699, 1032, 1047, 1064
Hsp92I GRCGYC 2 cut(s) 150, 1223
Hsp92II CATG 2 cut(s) 724, 1162
HspAI GCGC 3 cut(s) 117, 243, 1197
KflI GGGWCCC 2 cut(s) 874, 888
KroI GCCGGC 1 cut(s) 1219
KroNI GCCGGC 1 cut(s) 1221
KspAI GTTAAC 1 cut(s) 345
Kzo9I GATC 5 cut(s) 109, 384, 570, 918, 963
LmnI GCTCC 6 cut(s) 173, 224, 318, 696, 743, 1207
Lsp1109I GCAGC 1 cut(s) 1087
MaeII ACGT 7 cut(s) 105, 150, 612, 699, 1032, 1047, 1064
MaeIII GTNAC 5 cut(s) 256, 373, 549, 666, 695
MalI GATC 5 cut(s) 111, 386, 572, 920, 965
MboI GATC 5 cut(s) 109, 384, 570, 918, 963
MboII GAAGA 3 cut(s) 175, 352, 736
MhlI GDGCHC 6 cut(s) 527, 731, 804, 1110, 1190, 1235
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 583, 859, 1081
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 264, 284, 743
MmeI TCCRAC 3 cut(s) 106, 312, 810
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 1219
MroXI GAANNNNTTC 1 cut(s) 860
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 344, 1035, 1242
Msp20I TGGCCA 1 cut(s) 5
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 1 cut(s) 193
MvnI CGCG 3 cut(s) 117, 521, 1199
NaeI GCCGGC 1 cut(s) 1221
NdeII GATC 5 cut(s) 109, 384, 570, 918, 963
NgoMIV GCCGGC 1 cut(s) 1219
NlaIII CATG 2 cut(s) 724, 1162
NmeAIII GCCGAG 3 cut(s) 252, 278, 1047
NmuCI GTSAC 2 cut(s) 256, 373
NspI RCATGY 1 cut(s) 724
PaqCI CACCTGC 1 cut(s) 248
PciI ACATGT 1 cut(s) 720
PcsI WCGNNNNNNNCGW 3 cut(s) 1038, 1211, 1220
PctI GAATGC 1 cut(s) 179
PdiI GCCGGC 1 cut(s) 1221
PdmI GAANNNNTTC 1 cut(s) 860
PfeI GAWTC 1 cut(s) 457
Pfl23II CGTACG 1 cut(s) 903
PinAI ACCGGT 2 cut(s) 377, 947
PkrI GCNGC 4 cut(s) 178, 1077, 1167, 1183
Ple19I CGATCG 1 cut(s) 921
PleI GAGTC 3 cut(s) 263, 284, 743
PpsI GAGTC 3 cut(s) 263, 284, 743
Ppu21I YACGTR 2 cut(s) 700, 1048
PpuMI RGGWCCY 4 cut(s) 565, 601, 874, 888
PscI ACATGT 1 cut(s) 720
Psp1406I AACGTT 1 cut(s) 1064
Psp5II RGGWCCY 4 cut(s) 565, 601, 874, 888
Psp6I CCWGG 1 cut(s) 191
PspFI CCCAGC 3 cut(s) 172, 621, 891
PspGI CCWGG 1 cut(s) 191
PspLI CGTACG 1 cut(s) 903
PspOMI GGGCCC 3 cut(s) 523, 727, 800
PspPPI RGGWCCY 4 cut(s) 565, 601, 874, 888
PstI CTGCAG 1 cut(s) 1077
PvuI CGATCG 1 cut(s) 921
RsaI GTAC 4 cut(s) 905, 1031, 1046, 1070
RsaNI GTAC 4 cut(s) 904, 1030, 1045, 1069
Rsr2I CGGWCCG 1 cut(s) 380
RsrII CGGWCCG 1 cut(s) 380
SaqAI TTAA 3 cut(s) 344, 1035, 1242
SatI GCNGC 4 cut(s) 177, 1076, 1166, 1182
Sau3AI GATC 5 cut(s) 109, 384, 570, 918, 963
SchI GAGTC 3 cut(s) 264, 284, 743
SduI GDGCHC 6 cut(s) 527, 731, 804, 1110, 1190, 1235
SfcI CTRYAG 1 cut(s) 1073
SfiI GGCCNNNNNGGCC 1 cut(s) 660
SinI GGWCC 7 cut(s) 380, 565, 601, 783, 874, 888, 1053
SmaI CCCGGG 1 cut(s) 1111
SmlI CTYRAG 2 cut(s) 560, 1144
SmoI CTYRAG 2 cut(s) 560, 1144
Sse9I AATT 3 cut(s) 583, 859, 1081
TaaI ACNGT 5 cut(s) 443, 791, 908, 1004, 1132
TaiI ACGT 7 cut(s) 108, 153, 615, 702, 1035, 1050, 1067
TaqI TCGA 6 cut(s) 108, 747, 917, 1104, 1115, 1214
TasI AATT 3 cut(s) 583, 859, 1081
TauI GCSGC 3 cut(s) 179, 1168, 1184
TfiI GAWTC 1 cut(s) 457
Tru1I TTAA 3 cut(s) 344, 1035, 1242
Tru9I TTAA 3 cut(s) 344, 1035, 1242
TscAI CASTG 5 cut(s) 283, 454, 802, 1137, 1235
TseFI GTSAC 2 cut(s) 256, 373
TseI GCWGC 1 cut(s) 1075
Tsp45I GTSAC 2 cut(s) 256, 373
TspGWI ACGGA 5 cut(s) 407, 683, 730, 868, 1220
TspMI CCCGGG 1 cut(s) 1109
TspRI CASTG 5 cut(s) 283, 454, 802, 1137, 1235
VpaK11BI GGWCC 7 cut(s) 380, 565, 601, 783, 874, 888, 1053
XceI RCATGY 1 cut(s) 724
XmaI CCCGGG 1 cut(s) 1109
XmnI GAANNNNTTC 1 cut(s) 860
ZraI GACGTC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.