pycom17g12770

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0017344
Physical Location & Seq
Forward (+)
10492701 .. 10493348
648 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g12770.1

Sequence Viewer

Length: 648 bp
ATGGCGAAGGTAGAAGATAAAGGAGGTAGAATTCCAATGTTAATTGCAATGAAAGGCCACCCGGGCACAGGAAAATCCACCCTGGCCCACTCCTTATCCTTCACCCTCAAACTCCCTCTCATCGACAAAGACGACGTGCGCGACAAGCTCCAAGCTCTCCAGTCCCACCACGCCGCCGCTTCCTCCCTGCTCAACGACACGTCCTACAGCGTCGTCTGGCAAATCGCGTCCACGCAGCTCCGCCTGGGCCTCAGCGTCATCGTCGACTCGCCCCTCTCCCGTAGGGCCCACCTCGAGCACCTGACCCAGCTGGCGTCGTCGTGCGGGGCCCGCGTGCTGGTCGTGGAGTGTAAGCCTAAGGACGAGGCTGAGTGGCGCCGGAGGCTCGAGCGCCGCGCTGCGGCTGAGGGTGGAGCCGAATGGCACAAGCCGTCCACGTGGGGGGATATGGTGAGGCTGTTGGATGGGTACGGCGGGTGCACGGAATATGATTTGGGCGATGTTCCCAAGGTGGTTGTGGATACGACGGCTGCTGTTGGATTTGAGGAGATTGTATCCAGTGTGCTCGAGTTCATTGAAATGCACAGTGGTCAGCCAATGTCATCTGAACACGAAGGACTAAAACTTATGAATTGGAATAACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_33 Ten1_2

Protein Analysis

216

Amino Acids

23.61

Weight (kDa)

6.13

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 10 - 131 1.7e-06 Zeta toxin
AAA_33 PF13671 14 - 146 4.9e-21 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017344)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g38852
prunus_persica Prupe.3G178900_v2.0.a1
pyrus_communis pycom17g12730 pycom17g12770
rosa_chinensis RchiOBHm_Chr2g0152451
rosa_laevigata RLG00000020634
rosa_multiflora Rmu_sc0005366.1_g000014
rosa_roxburghii Rroxscaffold_2G00095710
rosa_samantha Rh2AG494600 Rh2BG506800 Rh2CG480800 Rh2DG518000
rosa_wichuraiana Rw2G040700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 375
AccI GTMKAC 1 cut(s) 264
AccII CGCG 4 cut(s) 141, 227, 333, 396
AciI CCGC 8 cut(s) 174, 177, 241, 324, 331, 394, 401, 474
AcsI RAATTY 1 cut(s) 30
AcvI CACGTG 1 cut(s) 438
AcyI GRCGYC 2 cut(s) 314, 376
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 4 cut(s) 68, 337, 400, 441
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 1 cut(s) 578
AjiI CACGTC 2 cut(s) 136, 201
AjnI CCWGG 2 cut(s) 81, 243
AluBI AGCT 4 cut(s) 148, 155, 238, 310
AluI AGCT 4 cut(s) 148, 155, 238, 310
Alw21I GWGCWC 3 cut(s) 300, 482, 567
Alw44I GTGCAC 1 cut(s) 478
Ama87I CYCGRG 4 cut(s) 61, 293, 386, 566
AoxI GGCC 5 cut(s) 55, 84, 247, 285, 327
ApaI GGGCCC 2 cut(s) 289, 331
ApaLI GTGCAC 1 cut(s) 478
ApeKI GCWGC 3 cut(s) 235, 398, 530
ApoI RAATTY 1 cut(s) 30
AspLEI GCGC 4 cut(s) 141, 378, 393, 398
AspS9I GGNCC 6 cut(s) 85, 247, 285, 286, 327, 328
AsuC2I CCSGG 2 cut(s) 62, 63
AsuHPI GGTGA 2 cut(s) 94, 463
AvaI CYCGRG 4 cut(s) 61, 293, 386, 566
AxyI CCTNAGG 1 cut(s) 357
BaeGI GKGCMC 4 cut(s) 68, 289, 331, 482
BanI GGYRCC 1 cut(s) 375
BanII GRGCYC 2 cut(s) 289, 331
BbrPI CACGTG 1 cut(s) 438
Bbv12I GWGCWC 3 cut(s) 300, 482, 567
BbvCI CCTCAGC 2 cut(s) 251, 405
BbvI GCAGC 3 cut(s) 247, 385, 517
BccI CCATC 1 cut(s) 458
BceAI ACGGC 3 cut(s) 415, 487, 543
BciT130I CCWGG 2 cut(s) 83, 245
BciVI GTATCC 2 cut(s) 514, 565
BcnI CCSGG 2 cut(s) 62, 63
BfmI CTRYAG 1 cut(s) 205
BfoI RGCGCY 2 cut(s) 379, 394
BfuI GTATCC 2 cut(s) 514, 565
BglI GCCNNNNNGGC 1 cut(s) 63
BisI GCNGC 7 cut(s) 174, 177, 236, 394, 399, 402, 531
BlsI GCNGC 7 cut(s) 175, 178, 237, 395, 400, 403, 532
Bme1390I CCNGG 4 cut(s) 62, 63, 83, 245
BmeT110I CYCGRG 4 cut(s) 61, 293, 386, 566
BmgBI CACGTC 2 cut(s) 136, 201
BmgT120I GGNCC 6 cut(s) 85, 247, 285, 286, 327, 328
BmiI GGNNCC 5 cut(s) 287, 328, 329, 377, 415
BmrFI CCNGG 4 cut(s) 62, 63, 83, 245
BpmI CTGGAG 1 cut(s) 143
Bpu10I CCTNAGC 2 cut(s) 251, 405
BpuMI CCSGG 2 cut(s) 62, 63
BsaAI YACGTR 1 cut(s) 438
BsaHI GRCGYC 2 cut(s) 314, 376
BsaJI CCNNGG 4 cut(s) 61, 81, 244, 507
Bsc4I CCNNNNNNNGG 4 cut(s) 68, 337, 400, 441
Bse1I ACTGG 2 cut(s) 160, 558
Bse21I CCTNAGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 54
BseBI CCWGG 2 cut(s) 83, 245
BseDI CCNNGG 4 cut(s) 61, 81, 244, 507
BseGI GGATG 1 cut(s) 469
BseLI CCNNNNNNNGG 4 cut(s) 68, 337, 400, 441
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 3 cut(s) 265, 360, 396
BseNI ACTGG 2 cut(s) 160, 558
BseRI GAGGAG 1 cut(s) 560
BseSI GKGCMC 4 cut(s) 68, 289, 331, 482
BseXI GCAGC 3 cut(s) 247, 385, 517
BseYI CCCAGC 1 cut(s) 306
Bsh1236I CGCG 4 cut(s) 141, 227, 333, 396
BshFI GGCC 5 cut(s) 57, 86, 249, 287, 329
BshNI GGYRCC 1 cut(s) 375
BsiHKAI GWGCWC 3 cut(s) 300, 482, 567
BsiHKCI CYCGRG 4 cut(s) 61, 293, 386, 566
BsiSI CCGG 2 cut(s) 62, 379
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 4 cut(s) 68, 337, 400, 441
BsmFI GGGAC 1 cut(s) 148
BsnI GGCC 5 cut(s) 57, 86, 249, 287, 329
BsoBI CYCGRG 4 cut(s) 61, 293, 386, 566
Bsp120I GGGCCC 2 cut(s) 285, 327
Bsp1286I GDGCHC 6 cut(s) 68, 289, 300, 331, 482, 567
BspACI CCGC 8 cut(s) 174, 177, 241, 324, 331, 394, 401, 474
BspANI GGCC 5 cut(s) 57, 86, 249, 287, 329
BspCNI CTCAG 3 cut(s) 264, 361, 397
BspFNI CGCG 4 cut(s) 141, 227, 333, 396
BspLI GGNNCC 5 cut(s) 287, 328, 329, 377, 415
BspT107I GGYRCC 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 54
BsrI ACTGG 2 cut(s) 160, 558
BssECI CCNNGG 4 cut(s) 61, 81, 244, 507
BssNI GRCGYC 2 cut(s) 314, 376
BssT1I CCWWGG 1 cut(s) 507
Bst2UI CCWGG 2 cut(s) 83, 245
Bst4CI ACNGT 1 cut(s) 587
BstACI GRCGYC 2 cut(s) 314, 376
BstBAI YACGTR 1 cut(s) 438
BstC8I GCNNGC 3 cut(s) 312, 331, 335
BstDEI CTNAG 4 cut(s) 251, 357, 369, 405
BstF5I GGATG 1 cut(s) 469
BstFNI CGCG 4 cut(s) 141, 227, 333, 396
BstH2I RGCGCY 2 cut(s) 379, 394
BstHHI GCGC 4 cut(s) 141, 378, 393, 398
BstMWI GCNNNNNNNGC 4 cut(s) 63, 145, 330, 382
BstNI CCWGG 2 cut(s) 83, 245
BstSCI CCNGG 4 cut(s) 60, 61, 81, 243
BstSFI CTRYAG 1 cut(s) 205
BstSLI GKGCMC 4 cut(s) 68, 289, 331, 482
BstUI CGCG 4 cut(s) 141, 227, 333, 396
BstV1I GCAGC 3 cut(s) 247, 385, 517
Bsu36I CCTNAGG 1 cut(s) 357
BsuI GTATCC 2 cut(s) 514, 565
BsuRI GGCC 5 cut(s) 57, 86, 249, 287, 329
BtgZI GCGATG 1 cut(s) 513
BtrI CACGTC 2 cut(s) 136, 201
BtsCI GGATG 1 cut(s) 469
BtsIMutI CAGTG 2 cut(s) 565, 592
Cac8I GCNNGC 3 cut(s) 312, 331, 335
CfoI GCGC 4 cut(s) 141, 378, 393, 398
Cfr13I GGNCC 6 cut(s) 85, 247, 285, 286, 327, 328
Cfr9I CCCGGG 1 cut(s) 61
CseI GACGC 4 cut(s) 199, 216, 244, 303
Csp6I GTAC 1 cut(s) 469
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 4 cut(s) 251, 357, 369, 405
DinI GGCGCC 1 cut(s) 377
EciI GGCGGA 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 507
Eco24I GRGCYC 2 cut(s) 289, 331
Eco72I CACGTG 1 cut(s) 438
Eco81I CCTNAGG 1 cut(s) 357
Eco88I CYCGRG 4 cut(s) 61, 293, 386, 566
EcoO109I RGGNCCY 2 cut(s) 285, 327
EcoRI GAATTC 1 cut(s) 30
EcoRII CCWGG 2 cut(s) 81, 243
EcoT14I CCWWGG 1 cut(s) 507
EcoT38I GRGCYC 2 cut(s) 289, 331
EgeI GGCGCC 1 cut(s) 377
EheI GGCGCC 1 cut(s) 377
ErhI CCWWGG 1 cut(s) 507
FaiI YATR 3 cut(s) 449, 489, 629
FaqI GGGAC 1 cut(s) 148
FauI CCCGC 3 cut(s) 317, 338, 467
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 7 cut(s) 174, 177, 236, 394, 399, 402, 531
FokI GGATG 1 cut(s) 476
FriOI GRGCYC 2 cut(s) 289, 331
Fsp4HI GCNGC 7 cut(s) 174, 177, 236, 394, 399, 402, 531
GlaI GCGC 4 cut(s) 140, 377, 392, 397
GluI GCNGC 7 cut(s) 174, 177, 236, 394, 399, 402, 531
GsaI CCCAGC 1 cut(s) 310
GsuI CTGGAG 1 cut(s) 143
HaeII RGCGCY 2 cut(s) 379, 394
HaeIII GGCC 5 cut(s) 57, 86, 249, 287, 329
HapII CCGG 2 cut(s) 62, 379
HgaI GACGC 4 cut(s) 199, 216, 244, 303
HhaI GCGC 4 cut(s) 141, 378, 393, 398
Hin1I GRCGYC 2 cut(s) 314, 376
Hin6I GCGC 4 cut(s) 139, 376, 391, 396
HinP1I GCGC 4 cut(s) 139, 376, 391, 396
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 1 cut(s) 266
HpaII CCGG 2 cut(s) 62, 379
HphI GGTGA 2 cut(s) 94, 463
Hpy166II GTNNAC 4 cut(s) 231, 265, 435, 480
Hpy188I TCNGA 1 cut(s) 607
Hpy8I GTNNAC 4 cut(s) 231, 265, 435, 480
Hpy99I CGWCG 6 cut(s) 137, 215, 266, 319, 322, 529
HpyAV CCTTC 2 cut(s) 109, 608
HpyCH4III ACNGT 1 cut(s) 587
HpyCH4IV ACGT 3 cut(s) 135, 200, 437
HpyCH4V TGCA 3 cut(s) 47, 480, 583
HpyF10VI GCNNNNNNNGC 4 cut(s) 63, 145, 330, 382
HpyF3I CTNAG 4 cut(s) 251, 357, 369, 405
HpySE526I ACGT 3 cut(s) 135, 200, 437
Hsp92I GRCGYC 2 cut(s) 314, 376
HspAI GCGC 4 cut(s) 139, 376, 391, 396
KasI GGCGCC 1 cut(s) 375
LmnI GCTCC 3 cut(s) 153, 243, 413
Lsp1109I GCAGC 3 cut(s) 247, 385, 517
MaeII ACGT 3 cut(s) 135, 200, 437
MboII GAAGA 1 cut(s) 26
MhlI GDGCHC 6 cut(s) 68, 289, 300, 331, 482, 567
MluCI AATT 4 cut(s) 30, 42, 631, 643
Mly113I GGCGCC 1 cut(s) 376
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 2 cut(s) 441, 517
MseI TTAA 1 cut(s) 41
MslI CAYNNNNRTG 1 cut(s) 578
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 2 cut(s) 62, 379
MspR9I CCNGG 4 cut(s) 62, 63, 83, 245
MteI GCGCNGCGC 1 cut(s) 394
MvaI CCWGG 2 cut(s) 83, 245
MvnI CGCG 4 cut(s) 141, 227, 333, 396
MwoI GCNNNNNNNGC 4 cut(s) 63, 145, 330, 382
NarI GGCGCC 1 cut(s) 376
NciI CCSGG 2 cut(s) 62, 63
NlaIV GGNNCC 5 cut(s) 287, 328, 329, 377, 415
PaeR7I CTCGAG 3 cut(s) 293, 386, 566
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 138
PkrI GCNGC 7 cut(s) 175, 178, 237, 395, 400, 403, 532
PleI GAGTC 1 cut(s) 260
PluTI GGCGCC 1 cut(s) 379
PmaCI CACGTG 1 cut(s) 438
PmlI CACGTG 1 cut(s) 438
PpsI GAGTC 1 cut(s) 260
Ppu21I YACGTR 1 cut(s) 438
Psp6I CCWGG 2 cut(s) 81, 243
PspCI CACGTG 1 cut(s) 438
PspFI CCCAGC 1 cut(s) 306
PspGI CCWGG 2 cut(s) 81, 243
PspN4I GGNNCC 5 cut(s) 287, 328, 329, 377, 415
PspOMI GGGCCC 2 cut(s) 285, 327
PspPI GGNCC 6 cut(s) 85, 247, 285, 286, 327, 328
PspXI VCTCGAGB 3 cut(s) 293, 386, 566
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 1 cut(s) 578
SalI GTCGAC 1 cut(s) 263
SaqAI TTAA 1 cut(s) 41
SatI GCNGC 7 cut(s) 174, 177, 236, 394, 399, 402, 531
Sau96I GGNCC 6 cut(s) 85, 247, 285, 286, 327, 328
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 4 cut(s) 62, 63, 83, 245
SduI GDGCHC 6 cut(s) 68, 289, 300, 331, 482, 567
SfcI CTRYAG 1 cut(s) 205
SfoI GGCGCC 1 cut(s) 377
Sfr274I CTCGAG 3 cut(s) 293, 386, 566
SlaI CTCGAG 3 cut(s) 293, 386, 566
SmaI CCCGGG 1 cut(s) 63
SmiMI CAYNNNNRTG 1 cut(s) 578
SmlI CTYRAG 3 cut(s) 293, 386, 566
SmoI CTYRAG 3 cut(s) 293, 386, 566
Sse9I AATT 4 cut(s) 30, 42, 631, 643
SsiI CCGC 8 cut(s) 174, 177, 241, 324, 331, 394, 401, 474
SspDI GGCGCC 1 cut(s) 375
StyD4I CCNGG 4 cut(s) 60, 61, 81, 243
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 1 cut(s) 587
TaiI ACGT 3 cut(s) 138, 203, 440
TaqI TCGA 5 cut(s) 123, 264, 294, 387, 567
TasI AATT 4 cut(s) 30, 42, 631, 643
TauI GCSGC 4 cut(s) 176, 179, 396, 404
Tru1I TTAA 1 cut(s) 41
Tru9I TTAA 1 cut(s) 41
TscAI CASTG 2 cut(s) 565, 592
TseI GCWGC 3 cut(s) 235, 398, 530
TspDTI ATGAA 3 cut(s) 65, 562, 644
TspGWI ACGGA 1 cut(s) 497
TspMI CCCGGG 1 cut(s) 61
TspRI CASTG 2 cut(s) 565, 592
VneI GTGCAC 1 cut(s) 478
XapI RAATTY 1 cut(s) 30
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XhoI CTCGAG 3 cut(s) 293, 386, 566
XmaI CCCGGG 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.