Rroxscaffold_2G00095710

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017344
Physical Location & Seq
Forward (+)
17089170 .. 17089775
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00095710.1

Sequence Viewer

Length: 606 bp
ATGGCCGACGAAGAAGGAAAAGGCTGTTCGGTTCCGGTGATAATAGCCATGAAAGGCCATCCCGGCACCGGTAAAACCACTCTGGCTCGTTCATTAGCTTCAACCCTCAAAATCCCTCTCATAGACAAAGACGACGTGCGCGACTCCACTCGGACTCTCCAGGAAGCCGGGGTCTCCTCTTCCTTGCTGAACGACATGTCGTACGGCGCCATCTGGCAGCTCGCGTCCACGCAGCTCCGCCTCGGGCTCAGCTTCGTCGTGGACTCGCCGCTCTCCCGGCGGGCCCACCTCGACCGGCTCCTCCAGATAGCGTCGTCCGGCGGGGCCCGCCTGGTGGTGGTGGAGTGCAGGCCGGCGGACGAGGACGAGTGGCGCCGGAGGCTCGAGCGGCGCGGCGGCGGTGATGATGAAGAGGAAGCCAGCTGGCACAAGCCGTCCACGTGGCGGGAGATGGAGAGGCTGCTGGAAAGCTACGACGGTTGCACGGATTATGATGCCGGAAACGTGCCAAAGGTGGTGGTGGATACTACGGCGGCTGTCGCGGTTCAAGAGCTTGTATACTCTGTAGTCGACTTCATCAAAACTCACACCGGTCTCCAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_33 Ten1_2

Protein Analysis

201

Amino Acids

22.03

Weight (kDa)

5.16

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_33 PF13671 15 - 140 1.5e-17 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017344)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g38852
prunus_persica Prupe.3G178900_v2.0.a1
pyrus_communis pycom17g12730 pycom17g12770
rosa_chinensis RchiOBHm_Chr2g0152451
rosa_laevigata RLG00000020634
rosa_multiflora Rmu_sc0005366.1_g000014
rosa_roxburghii Rroxscaffold_2G00095710
rosa_samantha Rh2AG494600 Rh2BG506800 Rh2CG480800 Rh2DG518000
rosa_wichuraiana Rw2G040700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 65, 206, 372
AccBSI CCGCTC 2 cut(s) 271, 388
AccI GTMKAC 2 cut(s) 558, 570
AccII CGCG 4 cut(s) 141, 224, 393, 542
AcoI YGGCCR 1 cut(s) 3
AcvI CACGTG 1 cut(s) 441
AcyI GRCGYC 2 cut(s) 207, 373
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 4 cut(s) 68, 334, 337, 444
AflIII ACRYGT 1 cut(s) 195
AgeI ACCGGT 2 cut(s) 68, 590
AgsI TTSAA 2 cut(s) 102, 548
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 2 cut(s) 159, 330
AluBI AGCT 7 cut(s) 98, 220, 235, 252, 423, 471, 553
AluI AGCT 7 cut(s) 98, 220, 235, 252, 423, 471, 553
Alw26I GTCTC 2 cut(s) 178, 599
Ama87I CYCGRG 2 cut(s) 242, 383
AoxI GGCC 5 cut(s) 3, 55, 282, 324, 350
ApaI GGGCCC 2 cut(s) 286, 328
ApeKI GCWGC 3 cut(s) 217, 232, 460
AsiGI ACCGGT 2 cut(s) 68, 590
AspLEI GCGC 4 cut(s) 141, 209, 375, 393
AspS9I GGNCC 4 cut(s) 282, 283, 324, 325
AsuC2I CCSGG 3 cut(s) 63, 169, 277
AsuHPI GGTGA 2 cut(s) 49, 413
AvaI CYCGRG 2 cut(s) 242, 383
BaeGI GKGCMC 2 cut(s) 286, 328
BanI GGYRCC 3 cut(s) 65, 206, 372
BanII GRGCYC 3 cut(s) 249, 286, 328
BbrPI CACGTG 1 cut(s) 441
BbvI GCAGC 3 cut(s) 229, 244, 447
BccI CCATC 3 cut(s) 66, 218, 445
BceAI ACGGC 3 cut(s) 220, 418, 546
BciT130I CCWGG 2 cut(s) 161, 332
BciVI GTATCC 1 cut(s) 517
BcnI CCSGG 3 cut(s) 63, 169, 277
BcoDI GTCTC 2 cut(s) 178, 599
BfmI CTRYAG 1 cut(s) 564
BfoI RGCGCY 2 cut(s) 210, 376
BfuI GTATCC 1 cut(s) 517
BglI GCCNNNNNGGC 1 cut(s) 63
BisI GCNGC 8 cut(s) 218, 233, 269, 389, 394, 397, 461, 534
BlpI GCTNAGC 1 cut(s) 248
BlsI GCNGC 8 cut(s) 219, 234, 270, 390, 395, 398, 462, 535
Bme1390I CCNGG 5 cut(s) 63, 161, 169, 277, 332
BmeT110I CYCGRG 2 cut(s) 242, 383
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 4 cut(s) 282, 283, 324, 325
BmiI GGNNCC 8 cut(s) 33, 67, 208, 284, 299, 325, 326, 374
BmrFI CCNGG 5 cut(s) 63, 161, 169, 277, 332
BmsI GCATC 1 cut(s) 484
BpmI CTGGAG 2 cut(s) 143, 287
Bpu1102I GCTNAGC 1 cut(s) 248
BpuMI CCSGG 3 cut(s) 63, 169, 277
BsaAI YACGTR 1 cut(s) 441
BsaHI GRCGYC 2 cut(s) 207, 373
BsaI GGTCTC 2 cut(s) 178, 599
BsaJI CCNNGG 2 cut(s) 168, 241
BsaWI WCCGGW 3 cut(s) 34, 68, 590
Bsc4I CCNNNNNNNGG 4 cut(s) 68, 334, 337, 444
Bse118I RCCGGY 4 cut(s) 68, 294, 352, 590
BseBI CCWGG 2 cut(s) 161, 332
BseDI CCNNGG 2 cut(s) 168, 241
BseGI GGATG 1 cut(s) 58
BseLI CCNNNNNNNGG 4 cut(s) 68, 334, 337, 444
BseMII CTCAG 1 cut(s) 262
BseRI GAGGAG 2 cut(s) 166, 290
BseSI GKGCMC 2 cut(s) 286, 328
BseXI GCAGC 3 cut(s) 229, 244, 447
BsgI GTGCAG 1 cut(s) 367
Bsh1236I CGCG 4 cut(s) 141, 224, 393, 542
Bsh1285I CGRYCG 1 cut(s) 295
BshFI GGCC 5 cut(s) 5, 57, 284, 326, 352
BshNI GGYRCC 3 cut(s) 65, 206, 372
BshTI ACCGGT 2 cut(s) 68, 590
BsiEI CGRYCG 1 cut(s) 295
BsiHKCI CYCGRG 2 cut(s) 242, 383
BsiWI CGTACG 1 cut(s) 201
BslI CCNNNNNNNGG 4 cut(s) 68, 334, 337, 444
BsmAI GTCTC 2 cut(s) 178, 599
BsnI GGCC 5 cut(s) 5, 57, 284, 326, 352
Bso31I GGTCTC 2 cut(s) 178, 599
BsoBI CYCGRG 2 cut(s) 242, 383
Bsp120I GGGCCC 2 cut(s) 282, 324
Bsp1286I GDGCHC 3 cut(s) 249, 286, 328
Bsp1720I GCTNAGC 1 cut(s) 248
BspANI GGCC 5 cut(s) 5, 57, 284, 326, 352
BspCNI CTCAG 1 cut(s) 261
BspFNI CGCG 4 cut(s) 141, 224, 393, 542
BspLI GGNNCC 8 cut(s) 33, 67, 208, 284, 299, 325, 326, 374
BspT107I GGYRCC 3 cut(s) 65, 206, 372
BspTNI GGTCTC 2 cut(s) 178, 599
BsrBI CCGCTC 2 cut(s) 271, 388
BsrFI RCCGGY 4 cut(s) 68, 294, 352, 590
BssAI RCCGGY 4 cut(s) 68, 294, 352, 590
BssECI CCNNGG 2 cut(s) 168, 241
BssNAI GTATAC 1 cut(s) 559
BssNI GRCGYC 2 cut(s) 207, 373
Bst1107I GTATAC 1 cut(s) 559
Bst2UI CCWGG 2 cut(s) 161, 332
Bst4CI ACNGT 1 cut(s) 479
Bst6I CTCTTC 2 cut(s) 184, 405
BstACI GRCGYC 2 cut(s) 207, 373
BstBAI YACGTR 1 cut(s) 441
BstC8I GCNNGC 7 cut(s) 222, 282, 328, 350, 354, 421, 425
BstDEI CTNAG 1 cut(s) 248
BstF5I GGATG 1 cut(s) 58
BstFNI CGCG 4 cut(s) 141, 224, 393, 542
BstH2I RGCGCY 2 cut(s) 210, 376
BstHHI GCGC 4 cut(s) 141, 209, 375, 393
BstMAI GTCTC 2 cut(s) 178, 599
BstMCI CGRYCG 1 cut(s) 295
BstMWI GCNNNNNNNGC 6 cut(s) 63, 277, 327, 379, 388, 539
BstNI CCWGG 2 cut(s) 161, 332
BstNSI RCATGY 1 cut(s) 199
BstSCI CCNGG 5 cut(s) 61, 159, 167, 275, 330
BstSFI CTRYAG 1 cut(s) 564
BstSLI GKGCMC 2 cut(s) 286, 328
BstUI CGCG 4 cut(s) 141, 224, 393, 542
BstV1I GCAGC 3 cut(s) 229, 244, 447
BstZ17I GTATAC 1 cut(s) 559
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 5 cut(s) 5, 57, 284, 326, 352
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 1 cut(s) 58
Cac8I GCNNGC 7 cut(s) 222, 282, 328, 350, 354, 421, 425
CfoI GCGC 4 cut(s) 141, 209, 375, 393
Cfr10I RCCGGY 4 cut(s) 68, 294, 352, 590
Cfr13I GGNCC 4 cut(s) 282, 283, 324, 325
CseI GACGC 2 cut(s) 213, 300
Csp6I GTAC 1 cut(s) 202
CspAI ACCGGT 2 cut(s) 68, 590
CspCI CAANNNNNGTGG 2 cut(s) 498, 533
CviAII CATG 2 cut(s) 49, 196
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 1 cut(s) 248
DinI GGCGCC 2 cut(s) 208, 374
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 184, 405
EarI CTCTTC 2 cut(s) 184, 405
EciI GGCGGA 2 cut(s) 227, 371
Eco24I GRGCYC 3 cut(s) 249, 286, 328
Eco31I GGTCTC 2 cut(s) 178, 599
Eco72I CACGTG 1 cut(s) 441
Eco88I CYCGRG 2 cut(s) 242, 383
EcoO109I RGGNCCY 1 cut(s) 324
EcoRII CCWGG 2 cut(s) 159, 330
EcoT38I GRGCYC 3 cut(s) 249, 286, 328
EgeI GGCGCC 2 cut(s) 208, 374
EheI GGCGCC 2 cut(s) 208, 374
FaeI CATG 2 cut(s) 52, 199
FaiI YATR 6 cut(s) 50, 122, 197, 492, 559, 604
FatI CATG 2 cut(s) 48, 195
FauI CCCGC 4 cut(s) 273, 314, 335, 438
FblI GTMKAC 2 cut(s) 558, 570
Fnu4HI GCNGC 8 cut(s) 218, 233, 269, 389, 394, 397, 461, 534
FokI GGATG 1 cut(s) 45
FriOI GRGCYC 3 cut(s) 249, 286, 328
Fsp4HI GCNGC 8 cut(s) 218, 233, 269, 389, 394, 397, 461, 534
GlaI GCGC 4 cut(s) 140, 208, 374, 392
GluI GCNGC 8 cut(s) 218, 233, 269, 389, 394, 397, 461, 534
GsuI CTGGAG 2 cut(s) 143, 287
HaeII RGCGCY 2 cut(s) 210, 376
HaeIII GGCC 5 cut(s) 5, 57, 284, 326, 352
HgaI GACGC 2 cut(s) 213, 300
HhaI GCGC 4 cut(s) 141, 209, 375, 393
Hin1I GRCGYC 2 cut(s) 207, 373
Hin1II CATG 2 cut(s) 52, 199
Hin6I GCGC 4 cut(s) 139, 207, 373, 391
HinP1I GCGC 4 cut(s) 139, 207, 373, 391
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HinfI GANTC 3 cut(s) 143, 154, 263
HphI GGTGA 2 cut(s) 49, 413
Hpy166II GTNNAC 5 cut(s) 228, 262, 438, 559, 571
Hpy188I TCNGA 1 cut(s) 153
Hpy188III TCNNGA 2 cut(s) 304, 548
Hpy8I GTNNAC 5 cut(s) 228, 262, 438, 559, 571
Hpy99I CGWCG 5 cut(s) 11, 137, 260, 316, 479
HpyAV CCTTC 1 cut(s) 8
HpyCH4III ACNGT 1 cut(s) 479
HpyCH4IV ACGT 3 cut(s) 135, 440, 504
HpyCH4V TGCA 2 cut(s) 348, 483
HpyF10VI GCNNNNNNNGC 6 cut(s) 63, 277, 327, 379, 388, 539
HpyF3I CTNAG 1 cut(s) 248
HpySE526I ACGT 3 cut(s) 135, 440, 504
Hsp92I GRCGYC 2 cut(s) 207, 373
Hsp92II CATG 2 cut(s) 52, 199
HspAI GCGC 4 cut(s) 139, 207, 373, 391
KasI GGCGCC 2 cut(s) 206, 372
KroI GCCGGC 1 cut(s) 352
KroNI GCCGGC 1 cut(s) 354
LmnI GCTCC 2 cut(s) 240, 303
Lsp1109I GCAGC 3 cut(s) 229, 244, 447
LweI GCATC 1 cut(s) 484
MaeII ACGT 3 cut(s) 135, 440, 504
MbiI CCGCTC 2 cut(s) 271, 388
MboII GAAGA 3 cut(s) 23, 171, 422
MhlI GDGCHC 3 cut(s) 249, 286, 328
Mly113I GGCGCC 2 cut(s) 207, 373
MlyI GAGTC 3 cut(s) 137, 148, 257
MroNI GCCGGC 1 cut(s) 352
MspA1I CMGCKG 1 cut(s) 423
MspR9I CCNGG 5 cut(s) 63, 161, 169, 277, 332
MvaI CCWGG 2 cut(s) 161, 332
MvnI CGCG 4 cut(s) 141, 224, 393, 542
MwoI GCNNNNNNNGC 6 cut(s) 63, 277, 327, 379, 388, 539
NaeI GCCGGC 1 cut(s) 354
NarI GGCGCC 2 cut(s) 207, 373
NciI CCSGG 3 cut(s) 63, 169, 277
NgoMIV GCCGGC 1 cut(s) 352
NlaIII CATG 2 cut(s) 52, 199
NlaIV GGNNCC 8 cut(s) 33, 67, 208, 284, 299, 325, 326, 374
NspI RCATGY 1 cut(s) 199
PaeR7I CTCGAG 1 cut(s) 383
PciI ACATGT 1 cut(s) 195
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PdiI GCCGGC 1 cut(s) 354
Pfl23II CGTACG 1 cut(s) 201
PfoI TCCNGGA 1 cut(s) 159
PinAI ACCGGT 2 cut(s) 68, 590
PkrI GCNGC 8 cut(s) 219, 234, 270, 390, 395, 398, 462, 535
PleI GAGTC 3 cut(s) 137, 148, 257
PluTI GGCGCC 2 cut(s) 210, 376
PmaCI CACGTG 1 cut(s) 441
PmlI CACGTG 1 cut(s) 441
PpsI GAGTC 3 cut(s) 137, 148, 257
Ppu21I YACGTR 1 cut(s) 441
PscI ACATGT 1 cut(s) 195
Psp6I CCWGG 2 cut(s) 159, 330
PspCI CACGTG 1 cut(s) 441
PspGI CCWGG 2 cut(s) 159, 330
PspLI CGTACG 1 cut(s) 201
PspN4I GGNNCC 8 cut(s) 33, 67, 208, 284, 299, 325, 326, 374
PspOMI GGGCCC 2 cut(s) 282, 324
PspPI GGNCC 4 cut(s) 282, 283, 324, 325
PspXI VCTCGAGB 1 cut(s) 383
PvuII CAGCTG 1 cut(s) 423
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SalI GTCGAC 1 cut(s) 569
SatI GCNGC 8 cut(s) 218, 233, 269, 389, 394, 397, 461, 534
Sau96I GGNCC 4 cut(s) 282, 283, 324, 325
SchI GAGTC 3 cut(s) 137, 148, 257
ScrFI CCNGG 5 cut(s) 63, 161, 169, 277, 332
SduI GDGCHC 3 cut(s) 249, 286, 328
SfaNI GCATC 1 cut(s) 484
SfcI CTRYAG 1 cut(s) 564
SfoI GGCGCC 2 cut(s) 208, 374
Sfr274I CTCGAG 1 cut(s) 383
SlaI CTCGAG 1 cut(s) 383
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
SspDI GGCGCC 2 cut(s) 206, 372
StyD4I CCNGG 5 cut(s) 61, 159, 167, 275, 330
TaaI ACNGT 1 cut(s) 479
TaiI ACGT 3 cut(s) 138, 443, 507
TaqI TCGA 3 cut(s) 291, 384, 570
TauI GCSGC 5 cut(s) 271, 391, 396, 399, 536
TseI GCWGC 3 cut(s) 217, 232, 460
TspDTI ATGAA 4 cut(s) 65, 81, 423, 565
TspGWI ACGGA 1 cut(s) 500
XceI RCATGY 1 cut(s) 199
XhoI CTCGAG 1 cut(s) 383
XmiI GTMKAC 2 cut(s) 558, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.