pycom17g21520

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0014172
Physical Location & Seq
Forward (+)
19893437 .. 19894601
1165 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
ATGGCTTTGGCACGTTTGGCGTTGAAGAAGTTGCACCAGAGCCAAAGGGTGCTTTCTCCACCACCTTTTTCTGCTGCTGCTTCTCTTCTAGGTCACGGTGTTGGTGAGAGGACAGTTGGTGGAGAGTGCAGAGGCAAAGTTGGCAATGAGATTGCGAAGAGGTTTTCTACGGCAGCTAACGACAAGGCATCCGATGAGAAATCGGAGAACAAAGATGTCGCTGTGTCTCAGGGCAAAAGGTCGAGGTTGTTCCCTAGAAGGCAACGCAGAAGGGGCCTTTGGAGGGACAATGACAGAGACTTCGCCCCTTCGCTTTATGGCCTTGGAAATGCACTTGTGCAAGCAACTGAGAACATAAACAGGCTGCTTGACAACCTAAACATATCACCATGGTCGCTGTCCGGGCGTGTCAAAGAGCAAAACGACAGCTACAAACTGCGGTATGATGTGCCAGGGCTTGCCAAGGAGGATGTGAAGATCATTGTTCATGACGGGTTTCTGGAAATCAAGGGAGAGCACAAAGACGAGTTCGAGGAAGGGTCGGATGATGAGTATTGGTCATCAAGGAGCTATGGTTACTATAACACAACTCTCAGTTTGCCTGATGATGCCAAAGTTGATGATATAAAGGCAGAGTTGAAGGATGGGGTACTGAGCATAACCATTCCTAAAACTGAGAAGCCTAAGAAGGAGGTGAAGGAGGTCAATATAAATTGA

Protein Analysis

239

Amino Acids

26.66

Weight (kDa)

8.78

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 137 - 238 5.5e-27 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52560 AT1G52560
fragaria_vesca FvH4_6g21590 FvH4_6g29880
malus_domestica MD17G1209800.v1.1
prunus_persica Prupe.3G085200_v2.0.a1
pyrus_communis pycom17g21520
rosa_chinensis RchiOBHm_Chr2g0135771
rosa_laevigata RLG00000019484
rosa_multiflora Rmu_sc0007848.1_g000021
rosa_roxburghii Rroxscaffold_2G00108840
rosa_rugosa Rorug02G0326300
rosa_samantha Rh2AG378000 Rh2BG384300 Rh2CG363700 Rh2DG400800
rosa_wichuraiana Rw2G030790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 439
AfaI GTAC 1 cut(s) 651
AfiI CCNNNNNNNGG 1 cut(s) 283
AgsI TTSAA 2 cut(s) 25, 640
AjnI CCWGG 1 cut(s) 451
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
AluBI AGCT 3 cut(s) 176, 429, 570
AluI AGCT 3 cut(s) 176, 429, 570
Alw21I GWGCWC 1 cut(s) 519
Alw26I GTCTC 2 cut(s) 231, 291
AoxI GGCC 2 cut(s) 274, 319
ApeKI GCWGC 4 cut(s) 74, 77, 173, 364
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AspS9I GGNCC 1 cut(s) 274
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 3 cut(s) 116, 378, 706
Bbv12I GWGCWC 1 cut(s) 519
BbvI GCAGC 4 cut(s) 61, 64, 185, 351
BccI CCATC 1 cut(s) 638
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 1 cut(s) 453
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 2 cut(s) 231, 291
BfaI CTAG 2 cut(s) 89, 255
BisI GCNGC 4 cut(s) 75, 78, 174, 365
BlsI GCNGC 4 cut(s) 76, 79, 175, 366
Bme1390I CCNGG 2 cut(s) 403, 453
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 1 cut(s) 275
BmrFI CCNGG 2 cut(s) 403, 453
BmsI GCATC 2 cut(s) 197, 598
BpuMI CCSGG 1 cut(s) 403
BsaJI CCNNGG 4 cut(s) 322, 389, 452, 462
BsaXI ACNNNNNCTCC 2 cut(s) 559, 589
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse3DI GCAATG 1 cut(s) 151
BseBI CCWGG 1 cut(s) 453
BseDI CCNNGG 4 cut(s) 322, 389, 452, 462
BseGI GGATG 4 cut(s) 188, 475, 550, 649
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMI GCAATG 1 cut(s) 151
BseMII CTCAG 5 cut(s) 242, 339, 607, 644, 666
BseXI GCAGC 4 cut(s) 61, 64, 185, 351
BsgI GTGCAG 1 cut(s) 148
BshFI GGCC 2 cut(s) 276, 321
BsiHKAI GWGCWC 1 cut(s) 519
BsiSI CCGG 1 cut(s) 402
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 1 cut(s) 283
BsmAI GTCTC 2 cut(s) 231, 291
BsmFI GGGAC 1 cut(s) 299
BsnI GGCC 2 cut(s) 276, 321
Bsp1286I GDGCHC 1 cut(s) 519
Bsp143I GATC 1 cut(s) 477
Bsp19I CCATGG 1 cut(s) 389
BspACI CCGC 1 cut(s) 439
BspANI GGCC 2 cut(s) 276, 321
BspCNI CTCAG 5 cut(s) 241, 340, 606, 645, 667
BspHI TCATGA 1 cut(s) 487
BspLI GGNNCC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 151
BssECI CCNNGG 4 cut(s) 322, 389, 452, 462
BssMI GATC 1 cut(s) 477
BssT1I CCWWGG 3 cut(s) 322, 389, 462
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 2 cut(s) 98, 115
Bst6I CTCTTC 2 cut(s) 90, 152
BstC8I GCNNGC 2 cut(s) 342, 459
BstDEI CTNAG 6 cut(s) 228, 348, 593, 653, 675, 684
BstDSI CCRYGG 1 cut(s) 389
BstF5I GGATG 4 cut(s) 188, 475, 550, 649
BstKTI GATC 1 cut(s) 480
BstMAI GTCTC 2 cut(s) 231, 291
BstMBI GATC 1 cut(s) 477
BstMWI GCNNNNNNNGC 4 cut(s) 17, 141, 273, 403
BstNI CCWGG 1 cut(s) 453
BstSCI CCNGG 2 cut(s) 401, 451
BstV1I GCAGC 4 cut(s) 61, 64, 185, 351
BsuRI GGCC 2 cut(s) 276, 321
BtgI CCRYGG 1 cut(s) 389
BtsCI GGATG 4 cut(s) 188, 475, 550, 649
Cac8I GCNNGC 2 cut(s) 342, 459
CciI TCATGA 1 cut(s) 487
Cfr13I GGNCC 1 cut(s) 274
Csp6I GTAC 1 cut(s) 650
CviAII CATG 2 cut(s) 390, 488
CviQI GTAC 1 cut(s) 650
DdeI CTNAG 6 cut(s) 228, 348, 593, 653, 675, 684
DpnI GATC 1 cut(s) 479
DpnII GATC 1 cut(s) 477
Eam1104I CTCTTC 2 cut(s) 90, 152
EarI CTCTTC 2 cut(s) 90, 152
Eco130I CCWWGG 3 cut(s) 322, 389, 462
EcoO109I RGGNCCY 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 451
EcoT14I CCWWGG 3 cut(s) 322, 389, 462
ErhI CCWWGG 3 cut(s) 322, 389, 462
FaeI CATG 2 cut(s) 393, 491
FaqI GGGAC 1 cut(s) 299
FatI CATG 2 cut(s) 389, 487
Fnu4HI GCNGC 4 cut(s) 75, 78, 174, 365
FokI GGATG 4 cut(s) 175, 482, 557, 656
Fsp4HI GCNGC 4 cut(s) 75, 78, 174, 365
FspBI CTAG 2 cut(s) 89, 255
GluI GCNGC 4 cut(s) 75, 78, 174, 365
HaeIII GGCC 2 cut(s) 276, 321
HapII CCGG 1 cut(s) 402
Hin1II CATG 2 cut(s) 393, 491
HpaII CCGG 1 cut(s) 402
HphI GGTGA 3 cut(s) 116, 378, 706
Hpy188I TCNGA 3 cut(s) 193, 205, 544
Hpy188III TCNNGA 2 cut(s) 488, 500
HpyAV CCTTC 7 cut(s) 252, 264, 318, 530, 634, 682, 691
HpyCH4III ACNGT 2 cut(s) 98, 115
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 4 cut(s) 34, 129, 332, 340
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 141, 273, 403
HpyF3I CTNAG 6 cut(s) 228, 348, 593, 653, 675, 684
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 2 cut(s) 393, 491
Kzo9I GATC 1 cut(s) 477
LmnI GCTCC 1 cut(s) 567
LpnPI CCDG 8 cut(s) 50, 215, 346, 415, 438, 465, 485, 615
Lsp1109I GCAGC 4 cut(s) 61, 64, 185, 351
LweI GCATC 2 cut(s) 197, 598
MaeI CTAG 2 cut(s) 89, 255
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 2 cut(s) 92, 575
MalI GATC 1 cut(s) 479
MboI GATC 1 cut(s) 477
MboII GAAGA 4 cut(s) 37, 77, 169, 487
MhlI GDGCHC 1 cut(s) 519
MluCI AATT 1 cut(s) 712
MmeI TCCRAC 1 cut(s) 522
MnlI CCTC 9 cut(s) 102, 125, 153, 237, 276, 460, 526, 685, 694
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 2 cut(s) 403, 453
MvaI CCWGG 1 cut(s) 453
MwoI GCNNNNNNNGC 4 cut(s) 17, 141, 273, 403
NciI CCSGG 1 cut(s) 403
NcoI CCATGG 1 cut(s) 389
NdeII GATC 1 cut(s) 477
NlaIII CATG 2 cut(s) 393, 491
NlaIV GGNNCC 1 cut(s) 275
NmuCI GTSAC 1 cut(s) 92
PagI TCATGA 1 cut(s) 487
PkrI GCNGC 4 cut(s) 76, 79, 175, 366
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 275
PspPI GGNCC 1 cut(s) 274
RsaI GTAC 1 cut(s) 651
RsaNI GTAC 1 cut(s) 650
SatI GCNGC 4 cut(s) 75, 78, 174, 365
Sau3AI GATC 1 cut(s) 477
Sau96I GGNCC 1 cut(s) 274
ScrFI CCNGG 2 cut(s) 403, 453
SduI GDGCHC 1 cut(s) 519
SfaNI GCATC 2 cut(s) 197, 598
Sse9I AATT 1 cut(s) 712
SsiI CCGC 1 cut(s) 439
SspMI CTAG 2 cut(s) 89, 255
StyD4I CCNGG 2 cut(s) 401, 451
StyI CCWWGG 3 cut(s) 322, 389, 462
TaaI ACNGT 2 cut(s) 98, 115
TaiI ACGT 1 cut(s) 16
TaqI TCGA 2 cut(s) 242, 531
TasI AATT 1 cut(s) 712
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 4 cut(s) 74, 77, 173, 364
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 1 cut(s) 476
XspI CTAG 2 cut(s) 89, 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.