pycom17g28000

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008305
Physical Location & Seq
Reverse (-)
26055443 .. 26058841
3399 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1659 bp
ATGAAGAAGGATCAGAACCAGAATCAGATGATGCATGTTTCATTGTTATTGTCACATCTGATTCTTCTGCTGCTCATTGATGGTGCTTTTGCTTCTGGGAAAAGTGATTATGCCTCAGCAGTTGGGGACCCAGGAATGAGGAGGGATGAGCTGAGAGTGGCCTTGGAAGCTTGGAACTTCTGCAATGAAGTTGGAGAAGAAGCACCTGATATGGGCAGTCCCAGAGCTGCTGATTGCTTTAATCTCACAAGTACTTCCTTAAAACACGGAGTGAGCGAAAAAGACAACTTGCTTGGGGTGGGGACTCCATTCCCAGGGATAAGCCAGGGGGCTTTGAACAATGTGGACCTTTATGCAGTAGAGAAGGAGCTGTATCTGGGTTCTCTGTGTCAAGTCCAAGACTCTCCAAAACCGTGGCATTTCTGGATGATTATGCTCAAAAATGGAAACTTTGACACAATGGCTGGGCTGTGCCCTTCCAATGGGAAAAGGACACCGCCTTTTAGCGATCCCGGAAGGTTCCCTTGCTTTGGGAAGGGCTGTATGAATCAACCCACTGTGGTCCACCAGAGGACTCTGCTAGATGGTAATACCACAATGAGAGGAAGTTTCAATGGGACTTATGATCTGGGATCCAACCCCAGCGATGGGCTGGATTCGATATCTTTCTACGAGGTGGAATGGAAGAAGAAACTTGGTGTTGGAAGCTGGGAGTTCAGGCACATACTCAAGACTTCAAAGAAGTATCCATGGCTGATGCTCTACCTCAGAGCTGATGCAACCCAAGGATATTCTGGTGGCTATCACTATGATACAAGAGGAATGCTCAAAATTCCTCCAGAGTCACCTAATTTTAAAGTCAGGCTGACTTTGGACATCAAACAAGGAGGAGGACCCAAGAGCCAGTTTTACCTCATTGACATTGGTAGCTGTTGGAAGAACAACGGCAAACCCTGTGACGGGGATGTGCTCACGGACGTGACCAGATACACGGAGATGATCATAAACCCGGAAACTCCTGCTTGGTGCAGCCCCACAGGTTTAGGCAATTGCCCGCCGTACCATATCACTCCAGACGACAGAAAAATTTATAGAAACGACACTGTCAACTTCCCATATGGGGCTTATCACTACTACTGTGCCCCGGGAAATGCTAAACATTTAGAGAAACCTGTAAGCGTATGTGATCCTTACAGTAATCCCCAGGCACAAGAGATAGTTCAGTTGCTCCCTCACCCCATCTGGGCCGAGTACGGGTATCCAACCAAACAAGGGCAGGGTTGGATTGGGGATGCTAGAACTTGGGAGCTTGATGTTGGTGGCCTATCCAGTAGACTCCACTACTACCAGGATCCAGGCACCACTCCTGCTAGAAGGATATGGGGATCAATTGATGTTGGTACAGAAATTTTTGTTAGTGACAAAGATGAAGTGGCAGACAAATTGCAGAAGACTTTGGTAGGGTGTCGTCTCCATTTGAAGCAAGAACCACCCACCACTCTGCTTTGCAACAAACCAAACCAATATTTGACAAAATTGGTCCAATCAAATGACTCGGATTTACACCACCATGTGCCATGTGGGGTTGGTGGTGGGAAACGCGCGAGCGCCCAAAGTTTGATGTGGAAACGAGGCTTTTTGAGTCACGGGATCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

553

Amino Acids

61.42

Weight (kDa)

6.97

Isoelectric Point (pI)

34.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7705 PF24804 39 - 480 7.2e-245 Domain of unknown function (DUF7705)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1358
AccI GTMKAC 1 cut(s) 1333
AccII CGCG 2 cut(s) 1602, 1604
AciI CCGC 2 cut(s) 497, 1055
AclWI GGATC 9 cut(s) 18, 503, 627, 640, 1181, 1346, 1359, 1393, 1658
AcsI RAATTY 3 cut(s) 831, 1086, 1407
AdeI CACNNNGTG 2 cut(s) 271, 1573
AfaI GTAC 4 cut(s) 253, 1061, 1253, 1402
AgsI TTSAA 4 cut(s) 337, 613, 738, 1480
AjiI CACGTC 1 cut(s) 979
AjnI CCWGG 6 cut(s) 130, 313, 324, 1203, 1347, 1354
AleI CACNNNNGTG 1 cut(s) 977
AluBI AGCT 8 cut(s) 151, 170, 227, 370, 708, 773, 930, 1309
AluI AGCT 8 cut(s) 151, 170, 227, 370, 708, 773, 930, 1309
Alw21I GWGCWC 1 cut(s) 972
Alw26I GTCTC 1 cut(s) 1475
AlwI GGATC 9 cut(s) 18, 503, 627, 640, 1181, 1346, 1359, 1393, 1658
Ama87I CYCGRG 1 cut(s) 1144
AoxI GGCC 3 cut(s) 159, 1245, 1321
ApeKI GCWGC 3 cut(s) 70, 227, 1029
ApoI RAATTY 3 cut(s) 831, 1086, 1407
AspLEI GCGC 2 cut(s) 1604, 1610
AspS9I GGNCC 6 cut(s) 127, 346, 562, 893, 1245, 1540
AsuC2I CCSGG 4 cut(s) 513, 1010, 1145, 1146
AsuHPI GGTGA 2 cut(s) 837, 1226
AvaI CYCGRG 1 cut(s) 1144
AvaII GGWCC 5 cut(s) 127, 346, 562, 893, 1540
BaeGI GKGCMC 2 cut(s) 476, 1144
BamHI GGATCC 2 cut(s) 632, 1351
BanI GGYRCC 1 cut(s) 1358
BbsI GAAGAC 1 cut(s) 1457
Bbv12I GWGCWC 1 cut(s) 972
BbvCI CCTCAGC 1 cut(s) 115
BbvI GCAGC 3 cut(s) 57, 214, 1041
BccI CCATC 4 cut(s) 74, 578, 641, 1247
BceAI ACGGC 2 cut(s) 961, 1042
BciT130I CCWGG 6 cut(s) 132, 315, 326, 1205, 1349, 1356
BciVI GTATCC 2 cut(s) 756, 1269
BclI TGATCA 1 cut(s) 999
BcnI CCSGG 4 cut(s) 513, 1010, 1145, 1146
BcoDI GTCTC 1 cut(s) 1475
BfaI CTAG 3 cut(s) 581, 1296, 1371
BfoI RGCGCY 1 cut(s) 1611
BfuI GTATCC 2 cut(s) 756, 1269
BisI GCNGC 3 cut(s) 71, 228, 1030
BlsI GCNGC 3 cut(s) 72, 229, 1031
BmcAI AGTACT 1 cut(s) 253
Bme18I GGWCC 5 cut(s) 127, 346, 562, 893, 1540
BmeT110I CYCGRG 1 cut(s) 1144
BmgBI CACGTC 1 cut(s) 979
BmgT120I GGNCC 6 cut(s) 127, 346, 562, 893, 1245, 1540
BmiI GGNNCC 7 cut(s) 128, 129, 521, 634, 895, 1353, 1360
BmsI GCATC 4 cut(s) 21, 747, 766, 1282
BpiI GAAGAC 1 cut(s) 1457
BplI GAGNNNNNCTC 2 cut(s) 810, 842
BpmI CTGGAG 2 cut(s) 822, 1056
Bpu10I CCTNAGC 1 cut(s) 115
BpuEI CTTGAG 1 cut(s) 713
BpuMI CCSGG 4 cut(s) 513, 1010, 1145, 1146
Bse1I ACTGG 2 cut(s) 904, 1329
Bse3DI GCAATG 1 cut(s) 190
BseBI CCWGG 6 cut(s) 132, 315, 326, 1205, 1349, 1356
BseGI GGATG 4 cut(s) 151, 432, 970, 1297
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 3 cut(s) 129, 143, 781
BseNI ACTGG 2 cut(s) 904, 1329
BseRI GAGGAG 2 cut(s) 154, 903
BseSI GKGCMC 2 cut(s) 476, 1144
BseXI GCAGC 3 cut(s) 57, 214, 1041
BseYI CCCAGC 3 cut(s) 464, 641, 708
BsgI GTGCAG 1 cut(s) 1048
Bsh1236I CGCG 2 cut(s) 1602, 1604
BshFI GGCC 3 cut(s) 161, 1247, 1323
BshNI GGYRCC 1 cut(s) 1358
BsiHKAI GWGCWC 1 cut(s) 972
BsiHKCI CYCGRG 1 cut(s) 1144
BsiSI CCGG 3 cut(s) 513, 1010, 1145
BslFI GGGAC 4 cut(s) 140, 204, 316, 631
BsmAI GTCTC 1 cut(s) 1475
BsmBI CGTCTC 1 cut(s) 1475
BsmFI GGGAC 4 cut(s) 140, 204, 316, 631
BsmI GAATGC 1 cut(s) 828
BsnI GGCC 3 cut(s) 161, 1247, 1323
BsoBI CYCGRG 1 cut(s) 1144
Bsp1286I GDGCHC 3 cut(s) 476, 972, 1144
Bsp143I GATC 9 cut(s) 10, 508, 625, 632, 999, 1186, 1351, 1385, 1650
Bsp19I CCATGG 1 cut(s) 749
BspACI CCGC 2 cut(s) 497, 1055
BspANI GGCC 3 cut(s) 161, 1247, 1323
BspCNI CTCAG 3 cut(s) 128, 144, 780
BspFNI CGCG 2 cut(s) 1602, 1604
BspLI GGNNCC 7 cut(s) 128, 129, 521, 634, 895, 1353, 1360
BspPI GGATC 9 cut(s) 18, 503, 627, 640, 1181, 1346, 1359, 1393, 1658
BspT107I GGYRCC 1 cut(s) 1358
BsrDI GCAATG 1 cut(s) 190
BsrI ACTGG 2 cut(s) 904, 1329
BssMI GATC 9 cut(s) 10, 508, 625, 632, 999, 1186, 1351, 1385, 1650
BssT1I CCWWGG 3 cut(s) 162, 749, 784
Bst2UI CCWGG 6 cut(s) 132, 315, 326, 1205, 1349, 1356
Bst4CI ACNGT 5 cut(s) 414, 559, 1105, 1139, 1196
BstC8I GCNNGC 2 cut(s) 1055, 1606
BstDEI CTNAG 3 cut(s) 115, 152, 767
BstDSI CCRYGG 2 cut(s) 413, 749
BstF5I GGATG 4 cut(s) 151, 432, 970, 1297
BstFNI CGCG 2 cut(s) 1602, 1604
BstH2I RGCGCY 1 cut(s) 1611
BstHHI GCGC 2 cut(s) 1604, 1610
BstKTI GATC 9 cut(s) 13, 511, 628, 635, 1002, 1189, 1354, 1388, 1653
BstMAI GTCTC 1 cut(s) 1475
BstMBI GATC 9 cut(s) 10, 508, 625, 632, 999, 1186, 1351, 1385, 1650
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstNI CCWGG 6 cut(s) 132, 315, 326, 1205, 1349, 1356
BstNSI RCATGY 1 cut(s) 38
BstSLI GKGCMC 2 cut(s) 476, 1144
BstUI CGCG 2 cut(s) 1602, 1604
BstV1I GCAGC 3 cut(s) 57, 214, 1041
BstV2I GAAGAC 1 cut(s) 1457
BstX2I RGATCY 2 cut(s) 632, 1351
BstXI CCANNNNNNTGG 1 cut(s) 414
BstYI RGATCY 2 cut(s) 632, 1351
BsuI GTATCC 2 cut(s) 756, 1269
BsuRI GGCC 3 cut(s) 161, 1247, 1323
BtgI CCRYGG 2 cut(s) 413, 749
BtgZI GCGATG 1 cut(s) 660
BtrI CACGTC 1 cut(s) 979
BtsCI GGATG 4 cut(s) 151, 432, 970, 1297
BtsIMutI CAGTG 2 cut(s) 555, 1101
Cac8I GCNNGC 2 cut(s) 1055, 1606
CfoI GCGC 2 cut(s) 1604, 1610
Cfr13I GGNCC 6 cut(s) 127, 346, 562, 893, 1245, 1540
Cfr9I CCCGGG 1 cut(s) 1144
Csp6I GTAC 4 cut(s) 252, 1060, 1252, 1401
CviAII CATG 4 cut(s) 35, 750, 1571, 1578
CviQI GTAC 4 cut(s) 252, 1060, 1252, 1401
DdeI CTNAG 3 cut(s) 115, 152, 767
DpnI GATC 9 cut(s) 12, 510, 627, 634, 1001, 1188, 1353, 1387, 1652
DpnII GATC 9 cut(s) 10, 508, 625, 632, 999, 1186, 1351, 1385, 1650
DraI TTTAAA 1 cut(s) 856
DraIII CACNNNGTG 2 cut(s) 271, 1573
Eco130I CCWWGG 3 cut(s) 162, 749, 784
Eco32I GATATC 1 cut(s) 663
Eco47I GGWCC 5 cut(s) 127, 346, 562, 893, 1540
Eco88I CYCGRG 1 cut(s) 1144
EcoO109I RGGNCCY 2 cut(s) 127, 893
EcoRII CCWGG 6 cut(s) 130, 313, 324, 1203, 1347, 1354
EcoRV GATATC 1 cut(s) 663
EcoT14I CCWWGG 3 cut(s) 162, 749, 784
EcoT22I ATGCAT 1 cut(s) 36
ErhI CCWWGG 3 cut(s) 162, 749, 784
Esp3I CGTCTC 1 cut(s) 1475
FaeI CATG 4 cut(s) 38, 753, 1574, 1581
FalI AAGNNNNNCTT 2 cut(s) 508, 540
FaqI GGGAC 4 cut(s) 140, 204, 316, 631
FatI CATG 4 cut(s) 34, 749, 1570, 1577
FauI CCCGC 1 cut(s) 1062
FauNDI CATATG 1 cut(s) 1117
FbaI TGATCA 1 cut(s) 999
FblI GTMKAC 1 cut(s) 1333
Fnu4HI GCNGC 3 cut(s) 71, 228, 1030
FokI GGATG 4 cut(s) 158, 439, 977, 1304
Fsp4HI GCNGC 3 cut(s) 71, 228, 1030
FspBI CTAG 3 cut(s) 581, 1296, 1371
GlaI GCGC 2 cut(s) 1603, 1609
GluI GCNGC 3 cut(s) 71, 228, 1030
GsaI CCCAGC 3 cut(s) 468, 645, 712
GsuI CTGGAG 2 cut(s) 822, 1056
HaeII RGCGCY 1 cut(s) 1611
HaeIII GGCC 3 cut(s) 161, 1247, 1323
HapII CCGG 3 cut(s) 513, 1010, 1145
HhaI GCGC 2 cut(s) 1604, 1610
Hin1II CATG 4 cut(s) 38, 753, 1574, 1581
Hin6I GCGC 2 cut(s) 1602, 1608
HinP1I GCGC 2 cut(s) 1602, 1608
HincII GTYRAC 1 cut(s) 1108
HindII GTYRAC 1 cut(s) 1108
HindIII AAGCTT 1 cut(s) 168
HpaII CCGG 3 cut(s) 513, 1010, 1145
HphI GGTGA 2 cut(s) 837, 1226
Hpy166II GTNNAC 4 cut(s) 346, 565, 1108, 1334
Hpy188I TCNGA 6 cut(s) 15, 27, 60, 770, 1558, 1658
Hpy188III TCNNGA 4 cut(s) 424, 730, 839, 1073
Hpy8I GTNNAC 4 cut(s) 346, 565, 1108, 1334
HpyAV CCTTC 5 cut(s) 358, 486, 510, 529, 1368
HpyCH4III ACNGT 5 cut(s) 414, 559, 1105, 1139, 1196
HpyCH4IV ACGT 1 cut(s) 978
HpyCH4V TGCA 7 cut(s) 34, 183, 356, 779, 1029, 1447, 1509
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 3 cut(s) 115, 152, 767
HpySE526I ACGT 1 cut(s) 978
Hsp92II CATG 4 cut(s) 38, 753, 1574, 1581
HspAI GCGC 2 cut(s) 1602, 1608
KflI GGGWCCC 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 999
Kzo9I GATC 9 cut(s) 10, 508, 625, 632, 999, 1186, 1351, 1385, 1650
LmnI GCTCC 3 cut(s) 367, 1233, 1306
Lsp1109I GCAGC 3 cut(s) 57, 214, 1041
LweI GCATC 4 cut(s) 21, 747, 766, 1282
MaeI CTAG 3 cut(s) 581, 1296, 1371
MaeII ACGT 1 cut(s) 978
MaeIII GTNAC 6 cut(s) 51, 843, 956, 979, 1418, 1643
MalI GATC 9 cut(s) 12, 510, 627, 634, 1001, 1188, 1353, 1387, 1652
MboI GATC 9 cut(s) 10, 508, 625, 632, 999, 1186, 1351, 1385, 1650
MboII GAAGA 7 cut(s) 16, 56, 209, 697, 700, 949, 1462
MfeI CAATTG 2 cut(s) 1048, 1389
MflI RGATCY 2 cut(s) 632, 1351
MhlI GDGCHC 3 cut(s) 476, 972, 1144
MluCI AATT 8 cut(s) 831, 850, 1048, 1086, 1389, 1407, 1442, 1535
MlyI GAGTC 7 cut(s) 298, 395, 568, 851, 1329, 1547, 1651
MmeI TCCRAC 6 cut(s) 172, 660, 682, 914, 1262, 1286
Mph1103I ATGCAT 1 cut(s) 36
MseI TTAA 3 cut(s) 240, 260, 855
MslI CAYNNNNRTG 3 cut(s) 977, 995, 1569
MspI CCGG 3 cut(s) 513, 1010, 1145
MunI CAATTG 2 cut(s) 1048, 1389
Mva1269I GAATGC 1 cut(s) 828
MvaI CCWGG 6 cut(s) 132, 315, 326, 1205, 1349, 1356
MvnI CGCG 2 cut(s) 1602, 1604
MwoI GCNNNNNNNGC 1 cut(s) 167
NciI CCSGG 4 cut(s) 513, 1010, 1145, 1146
NcoI CCATGG 1 cut(s) 749
NdeI CATATG 1 cut(s) 1117
NdeII GATC 9 cut(s) 10, 508, 625, 632, 999, 1186, 1351, 1385, 1650
NlaIII CATG 4 cut(s) 38, 753, 1574, 1581
NlaIV GGNNCC 7 cut(s) 128, 129, 521, 634, 895, 1353, 1360
NmeAIII GCCGAG 1 cut(s) 1273
NmuCI GTSAC 6 cut(s) 51, 843, 956, 979, 1418, 1643
NsiI ATGCAT 1 cut(s) 36
NspI RCATGY 1 cut(s) 38
OliI CACNNNNGTG 1 cut(s) 977
PasI CCCWGGG 1 cut(s) 314
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PctI GAATGC 1 cut(s) 828
PfeI GAWTC 4 cut(s) 22, 61, 547, 656
PflFI GACNNNGTC 1 cut(s) 1103
PfoI TCCNGGA 1 cut(s) 511
PkrI GCNGC 3 cut(s) 72, 229, 1031
PleI GAGTC 7 cut(s) 298, 395, 568, 850, 1329, 1547, 1650
PpsI GAGTC 7 cut(s) 298, 395, 568, 850, 1329, 1547, 1650
PpuMI RGGWCCY 2 cut(s) 127, 893
Psp5II RGGWCCY 2 cut(s) 127, 893
Psp6I CCWGG 6 cut(s) 130, 313, 324, 1203, 1347, 1354
PspFI CCCAGC 3 cut(s) 464, 641, 708
PspGI CCWGG 6 cut(s) 130, 313, 324, 1203, 1347, 1354
PspN4I GGNNCC 7 cut(s) 128, 129, 521, 634, 895, 1353, 1360
PspPI GGNCC 6 cut(s) 127, 346, 562, 893, 1245, 1540
PspPPI RGGWCCY 2 cut(s) 127, 893
PsuI RGATCY 2 cut(s) 632, 1351
PsyI GACNNNGTC 1 cut(s) 1103
RsaI GTAC 4 cut(s) 253, 1061, 1253, 1402
RsaNI GTAC 4 cut(s) 252, 1060, 1252, 1401
RseI CAYNNNNRTG 3 cut(s) 977, 995, 1569
SaqAI TTAA 3 cut(s) 240, 260, 855
SatI GCNGC 3 cut(s) 71, 228, 1030
Sau3AI GATC 9 cut(s) 10, 508, 625, 632, 999, 1186, 1351, 1385, 1650
Sau96I GGNCC 6 cut(s) 127, 346, 562, 893, 1245, 1540
ScaI AGTACT 1 cut(s) 253
SchI GAGTC 7 cut(s) 298, 395, 568, 851, 1329, 1547, 1651
SduI GDGCHC 3 cut(s) 476, 972, 1144
SfaNI GCATC 4 cut(s) 21, 747, 766, 1282
SinI GGWCC 5 cut(s) 127, 346, 562, 893, 1540
SmaI CCCGGG 1 cut(s) 1146
SmiMI CAYNNNNRTG 3 cut(s) 977, 995, 1569
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
Sse9I AATT 8 cut(s) 831, 850, 1048, 1086, 1389, 1407, 1442, 1535
SsiI CCGC 2 cut(s) 497, 1055
SspI AATATT 1 cut(s) 1526
SspMI CTAG 3 cut(s) 581, 1296, 1371
StyI CCWWGG 3 cut(s) 162, 749, 784
TaaI ACNGT 5 cut(s) 414, 559, 1105, 1139, 1196
TaiI ACGT 1 cut(s) 981
TaqI TCGA 1 cut(s) 659
TasI AATT 8 cut(s) 831, 850, 1048, 1086, 1389, 1407, 1442, 1535
TatI WGTACW 1 cut(s) 251
TfiI GAWTC 4 cut(s) 22, 61, 547, 656
Tru1I TTAA 3 cut(s) 240, 260, 855
Tru9I TTAA 3 cut(s) 240, 260, 855
TscAI CASTG 2 cut(s) 562, 1108
TseFI GTSAC 6 cut(s) 51, 843, 956, 979, 1418, 1643
TseI GCWGC 3 cut(s) 70, 227, 1029
Tsp45I GTSAC 6 cut(s) 51, 843, 956, 979, 1418, 1643
TspDTI ATGAA 5 cut(s) 17, 30, 201, 560, 1443
TspGWI ACGGA 3 cut(s) 282, 989, 1007
TspMI CCCGGG 1 cut(s) 1144
TspRI CASTG 2 cut(s) 562, 1108
Tth111I GACNNNGTC 1 cut(s) 1103
VpaK11BI GGWCC 5 cut(s) 127, 346, 562, 893, 1540
XapI RAATTY 3 cut(s) 831, 1086, 1407
XceI RCATGY 1 cut(s) 38
XcmI CCANNNNNNNNNTGG 3 cut(s) 649, 791, 1577
XmaI CCCGGG 1 cut(s) 1144
XmiI GTMKAC 1 cut(s) 1333
XspI CTAG 3 cut(s) 581, 1296, 1371
ZrmI AGTACT 1 cut(s) 253
Zsp2I ATGCAT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.