pycom420g00270

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0019626
Physical Location & Seq
Forward (+)
324204 .. 325568
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 861 bp
ATGTGCCAGAGTTCAGTGGAAAGCCATGTGTTCTATGTCAGCTACATTTATGGAGAGAAAGAATACTTGGCAAGACAAGGAAGGAAAGAAAAGCGGGCATGCAAGAAGCCTCAAATGGTGGCTAACATCAACAAACCCTTCCCAACCGGGTCCAACATTCGGATGTTTTTTTATTGGGATTTGGAAGATGTCCACTCCTCTCACGACGACGCTTTGGTGATCAAGGTTCAAATCTCCAACGCAATGGTCAGTTGCATCTTTGTAGACAATGGCTCCGGATGTGTTGATGTTCAATATAAAGCTAAAAGATGGAGAGAAGAGGCAGCTATGGCTATGTTTGGGGCCATAAATGACCTTTTCGAAGCCACCATAGTCCGGGCAAAGGACATTAGGATCCTGGTGGTGAACTGCGGAGTTCTAAATGCGACTCCATCATCCCTTTTAAATGCTTATCCTGGCACATATGCACTGGTGGTTAGCACAGAGGCTGTGTGCTATACATGGTACAAGGGCAACGATTTTGATATGCTCCTTCCTAATTGTTTCTTTCGAATAGGGGCTGCCTCCATTCTGCTCTCTAGCTCCGATGGTGTGCCAAATATGTACCCAAGCAGCGACCGGCAAGAAGGTGCTCGACAAAATACAGACCTAGAGCTTACGGAGTACATGGAGGCATCCGGGAAAACCCTGGAGCGATTTGGAAACACCTCTAGTAGTAGCAGGGGCGATTGGGTTTGGCAGCTCGCCTTCGGCTCAGGGTTCAAGTGTAACAGCGTCGTCTGGAAAGCTCTTAAGAATGTTGGGACGCCCAAGCAGAGTCCATGGCTTGATGGCAGCAAGTTGACAAAGAAGGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

287

Amino Acids

31.97

Weight (kDa)

8.53

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 101 - 145 1.7e-08 FAE1/Type III polyketide synthase-like protein
FAE1_CUT1_RppA PF08392 146 - 196 7.4e-15 FAE1/Type III polyketide synthase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52160 AT3G52160
malus_domestica MD04G1001400.v1.1 MD13G1285900.v1.1
prunus_persica Prupe.1G001400_v2.0.a1
pyrus_communis pycom04g00200 pycom420g00270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 264
AccIII TCCGGA 1 cut(s) 275
AciI CCGC 2 cut(s) 94, 411
AclWI GGATC 2 cut(s) 388, 401
AcyI GRCGYC 1 cut(s) 806
AfaI GTAC 3 cut(s) 506, 605, 665
AfiI CCNNNNNNNGG 3 cut(s) 159, 375, 382
AflII CTTAAG 1 cut(s) 791
AgsI TTSAA 3 cut(s) 230, 293, 763
AjnI CCWGG 3 cut(s) 396, 454, 687
AjuI GAANNNNNNNTTGG 2 cut(s) 50, 82
AluBI AGCT 7 cut(s) 42, 302, 326, 582, 655, 742, 788
AluI AGCT 7 cut(s) 42, 302, 326, 582, 655, 742, 788
Alw21I GWGCWC 1 cut(s) 634
AlwI GGATC 2 cut(s) 388, 401
AlwNI CAGNNNCTG 1 cut(s) 488
Aor13HI TCCGGA 1 cut(s) 275
AoxI GGCC 1 cut(s) 342
ApeKI GCWGC 5 cut(s) 323, 560, 612, 739, 834
AspS9I GGNCC 2 cut(s) 150, 342
AsuC2I CCSGG 3 cut(s) 148, 377, 679
AsuHPI GGTGA 2 cut(s) 229, 415
AsuII TTCGAA 2 cut(s) 360, 550
AvaII GGWCC 1 cut(s) 150
BamHI GGATCC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 634
BbvI GCAGC 5 cut(s) 335, 547, 624, 751, 846
BccI CCATC 4 cut(s) 303, 439, 581, 824
BciT130I CCWGG 3 cut(s) 398, 456, 689
BclI TGATCA 1 cut(s) 219
BcnI CCSGG 3 cut(s) 148, 377, 679
BfaI CTAG 3 cut(s) 579, 650, 711
BfrI CTTAAG 1 cut(s) 791
BisI GCNGC 5 cut(s) 324, 561, 613, 740, 835
BlsI GCNGC 5 cut(s) 325, 562, 614, 741, 836
Bme1390I CCNGG 6 cut(s) 148, 377, 398, 456, 679, 689
Bme18I GGWCC 1 cut(s) 150
BmgT120I GGNCC 2 cut(s) 150, 342
BmiI GGNNCC 4 cut(s) 151, 274, 343, 395
BmrFI CCNGG 6 cut(s) 148, 377, 398, 456, 679, 689
BmsI GCATC 2 cut(s) 264, 683
BpmI CTGGAG 1 cut(s) 710
Bpu10I CCTNAGC 1 cut(s) 754
Bpu14I TTCGAA 2 cut(s) 360, 550
BpuMI CCSGG 3 cut(s) 148, 377, 679
BsaHI GRCGYC 1 cut(s) 806
BsaJI CCNNGG 2 cut(s) 687, 821
BsaWI WCCGGW 1 cut(s) 275
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 375, 382
Bse118I RCCGGY 1 cut(s) 618
Bse1I ACTGG 1 cut(s) 474
Bse3DI GCAATG 1 cut(s) 249
BseAI TCCGGA 1 cut(s) 275
BseBI CCWGG 3 cut(s) 398, 456, 689
BseDI CCNNGG 2 cut(s) 687, 821
BseGI GGATG 4 cut(s) 168, 284, 434, 674
BseLI CCNNNNNNNGG 3 cut(s) 159, 375, 382
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 1 cut(s) 768
BseNI ACTGG 1 cut(s) 474
BseRI GAGGAG 1 cut(s) 187
BseXI GCAGC 5 cut(s) 335, 547, 624, 751, 846
Bsh1285I CGRYCG 1 cut(s) 619
BshFI GGCC 1 cut(s) 344
BsiEI CGRYCG 1 cut(s) 619
BsiHKAI GWGCWC 1 cut(s) 634
BsiSI CCGG 5 cut(s) 147, 276, 376, 619, 678
BslFI GGGAC 1 cut(s) 817
BslI CCNNNNNNNGG 3 cut(s) 159, 375, 382
BsmFI GGGAC 1 cut(s) 817
BsnI GGCC 1 cut(s) 344
Bsp119I TTCGAA 2 cut(s) 360, 550
Bsp1286I GDGCHC 1 cut(s) 634
Bsp13I TCCGGA 1 cut(s) 275
Bsp143I GATC 2 cut(s) 219, 393
Bsp19I CCATGG 1 cut(s) 821
BspACI CCGC 2 cut(s) 94, 411
BspANI GGCC 1 cut(s) 344
BspCNI CTCAG 1 cut(s) 767
BspEI TCCGGA 1 cut(s) 275
BspLI GGNNCC 4 cut(s) 151, 274, 343, 395
BspPI GGATC 2 cut(s) 388, 401
BspT104I TTCGAA 2 cut(s) 360, 550
BspTI CTTAAG 1 cut(s) 791
BsrDI GCAATG 1 cut(s) 249
BsrFI RCCGGY 1 cut(s) 618
BsrI ACTGG 1 cut(s) 474
BssAI RCCGGY 1 cut(s) 618
BssECI CCNNGG 2 cut(s) 687, 821
BssMI GATC 2 cut(s) 219, 393
BssNI GRCGYC 1 cut(s) 806
BssT1I CCWWGG 1 cut(s) 821
Bst2UI CCWGG 3 cut(s) 398, 456, 689
Bst6I CTCTTC 1 cut(s) 312
BstACI GRCGYC 1 cut(s) 806
BstAFI CTTAAG 1 cut(s) 791
BstBI TTCGAA 2 cut(s) 360, 550
BstC8I GCNNGC 3 cut(s) 96, 100, 744
BstDEI CTNAG 1 cut(s) 754
BstDSI CCRYGG 1 cut(s) 821
BstF5I GGATG 4 cut(s) 168, 284, 434, 674
BstKTI GATC 2 cut(s) 222, 396
BstMBI GATC 2 cut(s) 219, 393
BstMCI CGRYCG 1 cut(s) 619
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 3 cut(s) 398, 456, 689
BstNSI RCATGY 1 cut(s) 102
BstSCI CCNGG 6 cut(s) 146, 375, 396, 454, 677, 687
BstV1I GCAGC 5 cut(s) 335, 547, 624, 751, 846
BstX2I RGATCY 1 cut(s) 393
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 1 cut(s) 393
BsuRI GGCC 1 cut(s) 344
BtgI CCRYGG 1 cut(s) 821
BtsCI GGATG 4 cut(s) 168, 284, 434, 674
BtsIMutI CAGTG 2 cut(s) 21, 467
Cac8I GCNNGC 3 cut(s) 96, 100, 744
CaiI CAGNNNCTG 1 cut(s) 488
Cfr10I RCCGGY 1 cut(s) 618
Cfr13I GGNCC 2 cut(s) 150, 342
CseI GACGC 3 cut(s) 218, 763, 814
Csp6I GTAC 3 cut(s) 505, 604, 664
CviAII CATG 5 cut(s) 26, 99, 501, 667, 822
CviQI GTAC 3 cut(s) 505, 604, 664
DdeI CTNAG 1 cut(s) 754
DpnI GATC 2 cut(s) 221, 395
DpnII GATC 2 cut(s) 219, 393
DraI TTTAAA 1 cut(s) 444
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
Eco130I CCWWGG 1 cut(s) 821
Eco47I GGWCC 1 cut(s) 150
EcoRII CCWGG 3 cut(s) 396, 454, 687
EcoT14I CCWWGG 1 cut(s) 821
ErhI CCWWGG 1 cut(s) 821
FaeI CATG 5 cut(s) 29, 102, 504, 670, 825
FaqI GGGAC 1 cut(s) 817
FatI CATG 5 cut(s) 25, 98, 500, 666, 821
FauI CCCGC 1 cut(s) 87
FauNDI CATATG 1 cut(s) 463
FbaI TGATCA 1 cut(s) 219
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 5 cut(s) 324, 561, 613, 740, 835
FokI GGATG 4 cut(s) 175, 291, 421, 661
Fsp4HI GCNGC 5 cut(s) 324, 561, 613, 740, 835
FspBI CTAG 3 cut(s) 579, 650, 711
GluI GCNGC 5 cut(s) 324, 561, 613, 740, 835
GsuI CTGGAG 1 cut(s) 710
HaeIII GGCC 1 cut(s) 344
HapII CCGG 5 cut(s) 147, 276, 376, 619, 678
HgaI GACGC 3 cut(s) 218, 763, 814
Hin1I GRCGYC 1 cut(s) 806
Hin1II CATG 5 cut(s) 29, 102, 504, 670, 825
HincII GTYRAC 1 cut(s) 843
HindII GTYRAC 1 cut(s) 843
HinfI GANTC 2 cut(s) 427, 817
HpaII CCGG 5 cut(s) 147, 276, 376, 619, 678
HphI GGTGA 2 cut(s) 229, 415
Hpy166II GTNNAC 4 cut(s) 193, 265, 406, 843
Hpy188I TCNGA 2 cut(s) 162, 586
Hpy188III TCNNGA 3 cut(s) 203, 276, 781
Hpy8I GTNNAC 4 cut(s) 193, 265, 406, 843
Hpy99I CGWCG 3 cut(s) 209, 212, 779
HpyAV CCTTC 6 cut(s) 75, 148, 542, 620, 757, 844
HpyCH4V TGCA 3 cut(s) 102, 255, 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 1 cut(s) 754
Hsp92I GRCGYC 1 cut(s) 806
Hsp92II CATG 5 cut(s) 29, 102, 504, 670, 825
Kpn2I TCCGGA 1 cut(s) 275
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 2 cut(s) 219, 393
LmnI GCTCC 4 cut(s) 278, 534, 587, 691
Lsp1109I GCAGC 5 cut(s) 335, 547, 624, 751, 846
LweI GCATC 2 cut(s) 264, 683
MaeI CTAG 3 cut(s) 579, 650, 711
MaeIII GTNAC 1 cut(s) 767
MalI GATC 2 cut(s) 221, 395
MboI GATC 2 cut(s) 219, 393
MboII GAAGA 2 cut(s) 197, 329
MflI RGATCY 1 cut(s) 393
MhlI GDGCHC 1 cut(s) 634
MluCI AATT 1 cut(s) 538
MlyI GAGTC 2 cut(s) 421, 826
MmeI TCCRAC 2 cut(s) 177, 261
MnlI CCTC 7 cut(s) 120, 208, 313, 478, 574, 664, 718
MroI TCCGGA 1 cut(s) 275
MseI TTAA 2 cut(s) 443, 792
MslI CAYNNNNRTG 1 cut(s) 161
MspCI CTTAAG 1 cut(s) 791
MspI CCGG 5 cut(s) 147, 276, 376, 619, 678
MspR9I CCNGG 6 cut(s) 148, 377, 398, 456, 679, 689
MvaI CCWGG 3 cut(s) 398, 456, 689
MwoI GCNNNNNNNGC 1 cut(s) 329
NciI CCSGG 3 cut(s) 148, 377, 679
NcoI CCATGG 1 cut(s) 821
NdeI CATATG 1 cut(s) 463
NdeII GATC 2 cut(s) 219, 393
NlaIII CATG 5 cut(s) 29, 102, 504, 670, 825
NlaIV GGNNCC 4 cut(s) 151, 274, 343, 395
NspI RCATGY 1 cut(s) 102
NspV TTCGAA 2 cut(s) 360, 550
PaeI GCATGC 1 cut(s) 102
PfoI TCCNGGA 1 cut(s) 677
PkrI GCNGC 5 cut(s) 325, 562, 614, 741, 836
PleI GAGTC 2 cut(s) 421, 825
PpsI GAGTC 2 cut(s) 421, 825
Psp6I CCWGG 3 cut(s) 396, 454, 687
PspGI CCWGG 3 cut(s) 396, 454, 687
PspN4I GGNNCC 4 cut(s) 151, 274, 343, 395
PspPI GGNCC 2 cut(s) 150, 342
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 1 cut(s) 393
RsaI GTAC 3 cut(s) 506, 605, 665
RsaNI GTAC 3 cut(s) 505, 604, 664
RseI CAYNNNNRTG 1 cut(s) 161
SaqAI TTAA 2 cut(s) 443, 792
SatI GCNGC 5 cut(s) 324, 561, 613, 740, 835
Sau3AI GATC 2 cut(s) 219, 393
Sau96I GGNCC 2 cut(s) 150, 342
SchI GAGTC 2 cut(s) 421, 826
ScrFI CCNGG 6 cut(s) 148, 377, 398, 456, 679, 689
SduI GDGCHC 1 cut(s) 634
SfaNI GCATC 2 cut(s) 264, 683
SfuI TTCGAA 2 cut(s) 360, 550
SinI GGWCC 1 cut(s) 150
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 1 cut(s) 791
SmoI CTYRAG 1 cut(s) 791
SphI GCATGC 1 cut(s) 102
Sse9I AATT 1 cut(s) 538
SsiI CCGC 2 cut(s) 94, 411
SspMI CTAG 3 cut(s) 579, 650, 711
StyD4I CCNGG 6 cut(s) 146, 375, 396, 454, 677, 687
StyI CCWWGG 1 cut(s) 821
TaqI TCGA 3 cut(s) 360, 550, 634
TasI AATT 1 cut(s) 538
TatI WGTACW 1 cut(s) 663
Tru1I TTAA 2 cut(s) 443, 792
Tru9I TTAA 2 cut(s) 443, 792
TscAI CASTG 2 cut(s) 21, 474
TseI GCWGC 5 cut(s) 323, 560, 612, 739, 834
TspGWI ACGGA 1 cut(s) 674
TspRI CASTG 2 cut(s) 21, 474
Vha464I CTTAAG 1 cut(s) 791
VpaK11BI GGWCC 1 cut(s) 150
XceI RCATGY 1 cut(s) 102
XmiI GTMKAC 1 cut(s) 264
XspI CTAG 3 cut(s) 579, 650, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.